CN1268211C - Method of preventing and treating white ant using microorganism - Google Patents
Method of preventing and treating white ant using microorganism Download PDFInfo
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- CN1268211C CN1268211C CN 200310112436 CN200310112436A CN1268211C CN 1268211 C CN1268211 C CN 1268211C CN 200310112436 CN200310112436 CN 200310112436 CN 200310112436 A CN200310112436 A CN 200310112436A CN 1268211 C CN1268211 C CN 1268211C
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Abstract
The present invention relates to a method for preventing and killing white ants by using microorganisms, which is characterized in that home white ants are infected with the pathogenic bacteria of Xenorhabdus nematophilus or Photorhabdus luminescens, and the pathogenic fungus of Beauveria bassiana. Preparations containing the pathogenic bacteria and preparations containing the pathogenic fungus are respectively applied to or mixed to directly apply to a white ant group (white ant nests or places for white ants to move) or an identical white ant inducer (such as cellulose substances), so the home white ants are infected with the pathogenic bacteria or the pathogenic fungus early or late, or simultaneously. Due to the cooperated effect of the pathogenic bacteria and the pathogenic fungus, the effect on killing home white ants is obviously enhanced. The present invention is an effective approach for preventing and killing white ants.
Description
Technical field
The present invention relates to utilize the method for microbial control termite, especially utilize the pathogenetic bacteria Xenorhabdusnematophilus of artificial culture or the disease fungus beauveria bassiana Beauveriabassiana combination of Photorhabdus luminescens and artificial culture, the method for control coptotermes formosanus (Coptotermes formosanus).
Background technology
Coptotermes formosanus is the very strong social insect of harmfulness.At present, the common method of control termite has two classes: the one, and the termite prevention; The 2nd, termite control.The termite prevention is to utilize chemical agent to handle soil or timber structure, to walk quickly and keep away and to prevent termite damage; Or utilize physical barrier or medicament obstacle band, make termite can not invade all kinds of buildings and target hazardous material.Termite control is to use various chemical agents to handle by the position of termite damage; Perhaps be provided with and induce case to induce termite, use various chemical agents to be applied to then and induce in the case, but other termite that is in contact with it by the termite superinfection that medicament infects.For prevention and the control efficiency of keeping termite, use the chemical agent of high toxicity and high residue usually, as chlopyrifos, Niran and pyrethroid, arsenic (arsenic trioxide) and mirex (organochlorine insecticide) etc.
Many patents relate to the method (US Patent No.6397516) of using chemical agent or physical barrier prevention and control termites.The well-known side effect that the chemical agent of use high toxicity and high residue brings is contaminated environment and threatens the person poultry safety.Therefore, environmentally friendly and person poultry safety's biologic product has been used to termite control.
The natural enemy of termite comprises predacious birds, spider, lizard, centipede, ant; The parasitic mite of parasitism, parasitic fly, parasitic nematode etc.Several pathogenic microorganisms have been found to infect termite.Sugiura et al (US Patent No.5728573,1998) utilizes a kind of disease fungus Beauveria brongniartii control termites.Metarhizium belongs to and Beauveria genus disease fungus also is used for control termites (US Patent No.5141744; US Patent No.5512280; US Patent No.5595746; US Patent Application No.20010006632).The beauveria bassiana that Beauveria belongs to is invaded in the insect bodies from the epidermis of host insect, and grows in insect bodies, causes insect death.When infecting insect, the conidium that at first is disease fungus produces the invasion mycelia attached to the insect body surface after the spore germination, cause host's death at last.
Xenorhabdus and Photorhabdus belong to symbiotic bacteria and are located away from respectively that insect cause of disease Si Shi belongs to Steinernema and different little bar belongs to the Heterorhabditis nematode.The entomopathogenic nematode of these two genus is new bio insecticides.Why deadly insect is because Xenorhabdus and Photorhabdus belong to the effect of symbiotic bacteria to this class nematode.Nematode is with infection period worm attitude (infectivejuveniles, IJ) enter in the insect bodies with host's food or from natural opening (as anus, pore), the intersegmental membrane of insect, discharge the Xenorhabdus and the Photorhabdus that carry in the enteric cavity subsequently and belong to symbiotic bacteria.The toxin (virulence factor) of symbiotic bacteria secretion causes insect death.Originally, scientists is thought, Xenorhabdus belongs to and the symbiotic bacteria of Photorhabdus genus has only to be carried or inject by nematode and just brings into play insecticidal action (Akhurst.Journal of GeneralMicrobiology, 1980,121:303-309 when entering in the insect bodies; Bedding.Annals of Applied Biology, 1984,104:117-120; Forst ﹠amp; Nealson.Microbiological Review, 1996,60:21-43).Recent findings, symbiotic bacteria can by the oral insect that causes death separately (Li Suchun etc. the 6th national insecticidal microorganism academic discussion thesis summary set .1995.43; Dudney R.US Patent No.488820,1997; Jarrett et al.PCT Patent No.98/08388,1998; Ragni etal.PCT Patent No.98/05212,1998), separablely from fungal component go out archon (Ensign et al.PCT PatentNo.97/17432,1997; Bowen et al.Science, 1998,280:2129-2132; Ensign et al.US Patent No.072264,2000).This bacterioid has been used to prevent and treat fiery ant (US Patent No.5616318) and termite (US PatentApplication No.20030082147).
Although Xenorhabdus and Photorhabdus belong to bacterium, and beauveria bassiana Beauveria bassiana disease fungus has been applied to control termites, and the work of using this two classes biologic product control termites does not together appear in the newspapers.
Summary of the invention
The objective of the invention is to develop the method for the control termites of a kind of microorganism.
Author of the present invention finds, the collaborative use of Xenorhabdus nematophilus or Photorhabdus luminescens pathogenetic bacteria and beauveria bassiana Beauveria bassiana disease fungus has synergistic effect to the coptotermes formosanus control, thereby develop the method for a kind of effective microbial control termite, realized purpose of the present invention.
The present invention utilizes the method for microbial control termite, it is characterized in that making coptotermes formosanus to catch Xenorhabdus nematophilus (Xenorhabdus nematophilus) or luminous rod bacterium (Photorhabdus luminescens) pathogenetic bacteria and beauveria bassiana (Beauveria bassiana) disease fungus.
Preparation that the present invention can be by will containing described pathogenetic bacteria and the preparation that contains described disease fungus be applied to same termite population respectively or be applied to same termite induces in the case, makes coptotermes formosanus successively directly or indirectly catch described pathogenetic bacteria and described disease fungus; Be applied to termite population or be applied to termite after also can mixing and induce in the case, make coptotermes formosanus catch described pathogenetic bacteria and described disease fungus simultaneously by preparation that will contain described pathogenetic bacteria and the preparation that contains described disease fungus.Described preparation use the conventional method that adopts, for example place or the termite that is applied to termitarium or termite activity with conventional sprayer or other mean for applying (as injection, irrigation etc.) induced in the case.
Xenorhabdus nematophilus of the present invention and Photorhabdus luminescens bacterium belong to Xenorhabdus respectively and Photorhabdus belongs to, separate respectively from the Steinernematidae Si Shi of Si Shi section genus Steinernema and the different little bar of different Rhabditidae Heterorhabditidae and belong to Heterorhabditis insect pathogen infection phase nematode, in classification, belong to Enterobacteriaceae section at present.
The artificial culture of Xenorhabdus nematophilus of the present invention and Photorhabdus luminescens bacterium adopts conventional, existing technology, behind separation, the purifying, with synthetic medium (can cultivate the medium of this bacterioid) culture in vitro as peptone water medium or other, comprise with after fermentation tank or the reactor assembly cultivation, the bacterium liquid that is obtained can be directly or through separate, extract and the active principle that obtains of purifying as the component of the used preparation of the present invention, use in the present invention.
The artificial culture of beauveria bassiana Beauveria bassiana disease fungus of the present invention adopts conventional, existing technology, with synthetic medium (can cultivate the medium of this class fungi) culture in vitro as potato glucose medium commonly used or other, comprise with after fermentation tank or the reactor assembly cultivation, the spore that is obtained can be used as the component of the used preparation of the present invention, uses in the present invention.
Preparation of the present invention can be all formulations that are applicable to biologic product, as liquor, wetting powder etc., also can add adjuvant, is configured to different formulations as adsorbent, wetting agent, preservative, sticker, attractant, protectant and synergist etc.
The content of pathogenetic bacteria described in the described preparation and disease fungus does not have special requirement, the same with common biologic product, for the effect that obtains, it is enough that its content is wanted, a situation arises and quantity according to ant, and the pathogenetic bacteria described in the liquor is generally every milliliter 10
5～10
8Order of magnitude thalline scope, the best is every milliliter 10
7～10
8Order of magnitude thalline, described disease fungus are generally every milliliter 10
5～10
9The scope of order of magnitude spore, the best is every milliliter 10
7～10
9Order of magnitude spore; Pathogenetic bacteria described in the pulvis is generally every gram 10
5～10
9The scope of order of magnitude thalline, the best is every gram 10
7～10
9Order of magnitude thalline, described disease fungus are generally every gram 10
5～10
9The scope of order of magnitude spore, the best is every gram 10
7～10
9Order of magnitude spore.
Preparation of the present invention is induced in the case with place or termite that conventional sprayer or other mean for applying (as injection, irrigation etc.) are applied to termitarium or termite activity.
Described termite induces case to adopt conventional and existing technology setting, is made up of timber (as China fir) or cellulose substances that termite is liked, also can add the termite derivant (as saccharide solutions such as sucrose, and the secretion of symbiosis of termite fungi etc.).Induce case generally to be positioned near the termitarium or the termite activity place, attract termites is got timber or the cellulose substances that food is induced case, or attract termites is movable in inducing case.After termite touches biologic product, infected, but infected termite other termite individuality of cross-infection again causes the infection of whole nest termite, thereby reach the purpose of control termites.
The present invention is because collaborative described pathogenetic bacteria and the described disease fungus of having used, and when the killing effect of termite obviously is better than using described bacterium and fungi separately, sees embodiment for details.
Embodiment
Following experiment and operational instances are further to explanation of the present invention, should not be used as limitation of the present invention.
Example one: Xenorhabdus and Photorhabdus belong to bacterium, and the cultivation of beauveria bassiana Beauveria bassiana and formulation preparation, are used for embodiment 2～7.
Xenorhabdus and Photorhabdus belong to cultivation and the formulation preparation of bacterium.
In the 500mL triangular flask, add 100mL liquid nutrient medium (1% peptone, 0.5% NaCl), 121 ℃ of following autoclave sterilizations 30 minutes.Insert the nascent type fungal component bacterial classification of 1mL X.nematophilus or P.luminescens then, place under 25 ℃, 150r/min shaking table to cultivate 48 hours, (every milliliter 10 approximately of the bacterium liquid that obtains
7～10
8Thalline) can be used as aqua, be applied to control termites.This bacterioid also can fermentation tank or reactor cultivate in a large number.When using bacterium bacterium liquid, the personnel of this professional domain can a situation arises and quantity according to termite, and bacterium liquid is diluted to every milliliter 10
5～10
8The thalline scope is applied to control termites then.But best bacterial concentration is every milliliter 10
7～10
8Thalline.
After the bacterium liquid process centrifuge of being cultivated was centrifugal, the results bacterial body added filler (as wood chip, corn flour, diatomite etc.) then, can be mixed with pulvis.When using the bacterium pulvis, the personnel of this professional domain can a situation arises and quantity according to termite, and pulvis is made every gram 10
5～10
9The scope of thalline is applied to control termites then.But best pulvis concentration is every gram 10
7～10
9Thalline.
The cultivation of beauveria bassiana and formulation preparation:
Beauveria bassiana with potato glucose medium (PDA) or the rice that cooks as cultivating based on after cultivating 7～10 days in 25 ℃ of culturing room, culture plate or cultivate in the box and cover with mycelia and produce spore.The chief's spore and mycelia available water wash, and make liquor, directly are applied to termite.When using beauveria bassiana bacterium liquid, the personnel of this professional domain can a situation arises and quantity according to termite, and bacterium liquid is diluted to every milliliter 10
5～10
9The scope of spore is applied to control termites then.But best spore concentration is every milliliter 10
7～10
9Spore.
As after adding filler (as diatomite, corn flour etc.), can be made into pulvis and be applied to control termites again.When using the beauveria bassiana pulvis, the personnel of this professional domain can a situation arises and quantity according to termite, and pulvis is made every gram 10
5～10
9The scope of spore is applied to control termites then.But best pulvis concentration is every gram 10
7～10
9Spore.
Example 2: mix and use pathogenetic bacteria bacterium liquid and disease fungus spore liquid control coptotermes formosanus.
X.nematophilus bacterium or P.luminescens bacterium obtain (every milliliter 5 * 10 approximately of bacterium liquid after cultivating in the liquid nutrient medium (1% peptone, 0.5% NaCl)
8Thalline).Beauveria bassiana covers with mycelia and produces spore after cultivating 10 days with potato glucose medium (PDA) in 25 ℃ of culturing room on the flat board.The chief's spore washes with sterile water in the flat board, makes (every milliliter 5 * 10 approximately of liquor
7Spore).(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 10% wood chip (also through autoclave sterilization), get 0.5 milliliter of bacterium bacterium liquid and 0.5 milliliter of fungal spore liquid mixing, add in the wood chip then in the flat board of each autoclave sterilization.In flat board, add again 20 not by bacterium and fungal infection worker ant.In flat board, only add bacterium bacterium liquid, or only add fungal spore liquid, or the termite that does not contact biologic product places same culture dish in contrast.The repetition of 4 flat boards is established in each processing.All flat boards are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 1 to the control efficiency of coptotermes formosanus.As can be seen from Table 1, mix to use bacterium and fungal spore liquid to the control efficiency of coptotermes formosanus greater than using bacterium bacterium liquid or fungal spore liquid separately.
Table 1 mixes the control efficiency of using pathogenetic bacteria bacterium liquid and disease fungus spore liquid control coptotermes formosanus
| Various bacterial strains or strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | The X.nematophilus+ beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 16％ | 18％ | 46％ | 88％ | 91％ | 7％ |
Example 3: mix and use pathogenetic bacteria and disease fungus pulvis control coptotermes formosanus.
(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 10% wood chip (also through autoclave sterilization),, get 0.5 gram bacterium pulvis and (contain every gram 10 approximately in the flat board of each autoclave sterilization according to the pulvis of example one described preparation
8Thalline) and 0.5 gram fungal spore pulvis (contain every gram 10 approximately
8Spore) mixing adds in the wood chip then.In flat board, add again 20 not by bacterium and fungal infection worker ant.In flat board, only add the bacterium pulvis, or only add beauveria bassiana spore pulvis, or the termite that does not contact biologic product places same culture dish in contrast.The repetition of 4 flat boards is established in each processing.All flat boards are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 2 to the control efficiency of coptotermes formosanus.As can be seen from Table 2, mix to use bacterium and fungal spore pulvis to the control efficiency of coptotermes formosanus greater than the control efficiency of using bacterium pulvis or fungal spore pulvis separately.
Table 2 mixes the control efficiency of using pathogenetic bacteria pulvis and disease fungus spore pulvis control coptotermes formosanus
| Various bacterial strains or strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | The X.nematophilus+ beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 15％ | 16％ | 39％ | 80％ | 83％ | 7％ |
Example 4: use pathogenetic bacteria bacterium liquid and disease fungus pulvis respectively and make coptotermes formosanus successively directly catch bacterium and fungi.
(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 50% wood chip (also through autoclave sterilization), in wood chip, sneak into (every milliliter 5 * 10 approximately of 0.5 milliliter of X.nematophilus or P.luminescens bacterium bacterium liquid then in the flat board of each autoclave sterilization
8Thalline).In flat board, add 20 again and scribbled fungi pulvis (every approximately gram 10
9The worker ant of creeping one minute in flat board spore).The worker ant of creeping one minute in the fungi flat board has fungal spore on one's body.In flat board, only add 0.5 milliliter of X.nematophilus or P.luminescens bacterium bacterium liquid, or only add 20 and long the worker ant of creeping a minute in the flat board of fungi has been arranged, or the termite that does not contact fungal spore places same culture dish in contrast.The repetition of 4 flat boards is established in each processing.All culture dishes are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 3 to the control efficiency of coptotermes formosanus.As can be seen from Table 3, use various bacterial strains the control efficiency of coptotermes formosanus to be not so good as to be used in combination the control efficiency of bacterial isolates and fungal bacterial strain separately.Coptotermes formosanus get the food be mixed with X.nematophilus or P.luminescens bacterium bacterium liquid after, these bacteriogenic toxicants have lethal effect to coptotermes formosanus, the immune system of perhaps destroying coptotermes formosanus makes fungi be easy to play a role.
Pathogenetic bacteria bacterium liquid used respectively by table 3 and the disease fungus pulvis makes coptotermes formosanus successively directly catch the control efficiency of bacterium and fungi
| Various bacterial strains and strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | X.nematophilus+beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 16％ | 18％ | 69％ | 94％ | 97％ | 7％ |
Example 5: use pathogenetic bacteria bacterium liquid and disease fungus spore liquid respectively and make coptotermes formosanus diffusion (indirectly) catch bacterium and fungi.
(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 50% wood chip (also through autoclave sterilization), in wood chip, sneak into (every milliliter 10 approximately of 0.5 milliliter of X.nematophilus or P.luminescens bacterium bacterium liquid then in the flat board of each autoclave sterilization
7Thalline).In flat board, add 19 worker ants and 1 again and scribbled (every milliliter 10 approximately of fungi liquid
9The worker ant of creeping one minute in flat board spore).The worker ant of creeping one minute in the fungi flat board has fungal spore on one's body.By contact, the termite that has fungal spore can be infected spore to other healthy termite individuality, causes cross-infection.In flat board, only add 0.5 milliliter of X.nematophilus or P.luminescens bacterium bacterium liquid, or only add 1 and the worker ant of creeping a minute in the flat board of fungi has been arranged, or the termite that does not contact fungal spore places same culture dish in contrast long.The repetition of 4 flat boards is established in each processing.All culture dishes are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 4 to the control efficiency of coptotermes formosanus.As can be seen from Table 4,1 headband has the coptotermes formosanus of fungal spore to need the time to remove other termite individuality in the cross-infection flat board, but, measure the 5th day the check result in back and also show, use various bacterial strains the control efficiency of coptotermes formosanus to be not so good as to be used in combination the control efficiency of bacterial isolates and fungal bacterial strain separately.
Pathogenetic bacteria bacterium liquid used respectively by table 4 and the disease fungus spore liquid makes the coptotermes formosanus diffusion catch the control efficiency of bacterium and fungi
| Various bacterial strains and strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | The X.nematophilus+ beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 13％ | 14％ | 48％ | 67％ | 71％ | 7％ |
Example 6: use pathogenetic bacteria pulvis and disease fungus pulvis to make coptotermes formosanus successively directly catch bacterium and fungi respectively.
(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 50% wood chip (also through autoclave sterilization) in the flat board of each autoclave sterilization, according to the pulvis of example one described preparation, get 0.5 gram X.nematophilus or P.luminescens bacterium bacterium liquid and (contain every gram 10 approximately
7Thalline).In flat board, add 20 again and scribbled fungi pulvis (every approximately gram 10
7The worker ant of creeping one minute in flat board spore), the worker ant of creeping a minute in the fungi flat board has fungal spore on one's body.In flat board, only add 0.5 gram X.nematophilus or P.luminescens bacterium bacterium liquid, or only add 20 and the worker ant of creeping a minute in the flat board of fungi has been arranged, or the termite that does not contact fungal spore places same culture dish in contrast long.The repetition of 4 flat boards is established in each processing.All culture dishes are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 5 to the control efficiency of coptotermes formosanus.As can be seen from Table 5, use various bacterial strains the control efficiency of coptotermes formosanus to be not so good as to be used in combination the control efficiency of bacterial isolates and fungal bacterial strain separately.Coptotermes formosanus get the food be mixed with X.nematophilus or P.luminescens bacterium bacterium liquid after, these bacteriogenic toxicants have lethal effect to coptotermes formosanus, the immune system of perhaps destroying coptotermes formosanus makes fungi be easy to play a role.
The pathogenetic bacteria pulvis used respectively by table 5 and the disease fungus pulvis makes coptotermes formosanus successively directly catch the control efficiency of bacterium and fungi
| Various bacterial strains and strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | X.nematophilus+beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 8％ | 11％ | 34％ | 59％ | 63％ | 7％ |
Example 7: use pathogenetic bacteria pulvis and disease fungus liquor respectively and make coptotermes formosanus diffusion (indirectly) catch bacterium and fungi.
(add one deck middling speed filter paper and 1 gram water content in the diameter=9cm) and be 50% wood chip (also through autoclave sterilization), in wood chip, sneak into 0.5 gram X.nematophilus or P.luminescens bacterium pulvis (every approximately gram 10 then in the flat board of each autoclave sterilization
9Thalline).In flat board, add 19 worker ants and 1 again and scribbled (every milliliter 10 approximately of fungi liquid
9The worker ant of creeping one minute in flat board spore).The worker ant of creeping one minute in the fungi flat board has fungal spore on one's body.By contact, the termite that has fungal spore can be infected spore to other healthy termite individuality, causes cross-infection.In flat board, only add 0.5 gram X.nematophilus or P.luminescens bacterium pulvis, or only add 1 and the worker ant of creeping a minute in the flat board of fungi has been arranged, or the termite that does not contact fungal spore places same culture dish in contrast long.The repetition of 4 flat boards is established in each processing.All culture dishes are sealed to seal film, place under 25 ℃.Check the death condition of termite every day, record termite death toll.
Measure back the 5th day, each bacterial strain and strain combinations see Table 6 to the control efficiency of coptotermes formosanus.As can be seen from Table 6,1 headband has the coptotermes formosanus of fungal spore to need the time to remove other termite individuality in the cross-infection flat board, but, measure the 5th day the check result in back and also show, use various bacterial strains the control efficiency of coptotermes formosanus to be not so good as to be used in combination the control efficiency of bacterial isolates and fungal bacterial strain separately.
Pathogenetic bacteria bacterium liquid used respectively by table 6 and the disease fungus spore liquid makes the coptotermes formosanus diffusion catch the control efficiency of bacterium and fungi
| Various bacterial strains and strain combinations | ||||||
| X.nematophilus | P.luminescens | Beauveria bassiana | The X.nematophilus+ beauveria bassiana | The P.luminescens+ beauveria bassiana | Contrast | |
| Lethality | 18％ | 20％ | 48％ | 79％ | 80％ | 7％ |
Claims (4)
1. a method of utilizing the microbial control termite is characterized in that making coptotermes formosanus to catch Xenorhabdus nematophilus (Xenorhabdus nematophilus) or luminous rod bacterium (Photorhabdus luminescens) pathogenetic bacteria and beauveria bassiana (Beauveria bassiana) disease fungus.
2. the method for utilizing the microbial control termite according to claim 1, the preparation that it is characterized in that the preparation by will containing described pathogenetic bacteria and contain described disease fungus is applied to same termite population respectively or is applied to same termite induces in the case, make coptotermes formosanus successively directly or letter connect and catch described pathogenetic bacteria and described disease fungus.
3. the method for utilizing the microbial control termite according to claim 1, it is characterized in that inducing in the case, make coptotermes formosanus catch described pathogenetic bacteria and described disease fungus simultaneously by being applied to termite population or being applied to termite after preparation that will contain described pathogenetic bacteria and the preparation mixing that contains described disease fungus.
4. according to claim 2 or the 3 described methods of utilizing the microbial control termite, it is characterized in that described preparation is liquor or pulvis; Contain described pathogenetic bacteria 10 in the described liquor
7～10
8Order of magnitude thalline/milliliter contains described disease fungus 10
7～10
9Order of magnitude spore/milliliter; Contain described pathogenetic bacteria 10 in the described pulvis
7～10
9Order of magnitude thalline/gram contains described disease fungus 10
7～10
9Order of magnitude spore/gram.
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