CN1267831A - Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever - Google Patents
Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever Download PDFInfo
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- CN1267831A CN1267831A CN 99113544 CN99113544A CN1267831A CN 1267831 A CN1267831 A CN 1267831A CN 99113544 CN99113544 CN 99113544 CN 99113544 A CN99113544 A CN 99113544A CN 1267831 A CN1267831 A CN 1267831A
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Abstract
In the detection, Hantaan virus resisting IgG antibody in serum is taken as detected object, and the detection process includes the following steps: encapsulating purified Hantaan virus nucleoprotein onto cellulose nitrate to form cellolose nitrate detection film with dot sample area encapsulating Hantaan virus nucleoprotein; preparing colloidal gold-antihuman IgG antibody conjugant with non-human anti human IgG antibody and colloidal gold as raw material; dropping serum sample to be detected into the dot sample area; washing the dropped dot sample area; dropping the colloidal gold-anti human IgG antibody conjugant to the dot sample area; and washing the dot sample area. The present invention can detect nephrotic syndrome and hemorrhagic fever fast, accurately and simply.
Description
The present invention relates to pestology, molecular biology and field of immunology.More specifically, the present invention relates to the detection method and the detection kit of hemorrhagic fever with renal syndrome.
Hemorrhagic fever with renal syndrome (HFRS) is the human acute infectious disease that is caused by some virus in Kenya's Viraceae Hantavirus (Hanta Virus).Morbidity back mortality ratio is very high, and therefore early stage correct diagnosis is very necessary.In China, renal syndrome-hemorrhagic fever (HFRS) is to endanger maximum a kind of infectious disease except that virus hepatitis, and this sick epidemic-stricken area scope relates to Europe, Asia and Africa etc. all over the world, and the number of the infected of China does not also have minimizing trend.In the area, PVG, owing to increasing of population from other places, the morbidity of HFRS is in the majority not down.Therefore, to the early stage quick diagnosis of this disease and treatment in time, the people's life and health had realistic meaning.
Hantaan virus is the pathogen of HFRS, the domestic immunofluorescence technique commonly used of this viral serological diagnosis and enzyme linked immune assay (ELISA) detection specificity IgG antibody and IgM, all there is certain limitation, at least also want 4 hours detection time, the high density granular agglutination test (HDPA) of external exploitation can be finished detection in 1 hour, but did not generally promote at home as yet.
Spot gold immunity percolation test (dripping golden method) is up-to-date advanced person, rapid technology, once is successfully used to the detection of multiple infectious disease, appears on the market but Shang Weijian is used for the kit of HFRS detection.Dripping golden method and have characteristics such as (can finish) easy and simple to handle, quick and specificity height in a few minutes, therefore is that the early stage quick diagnosis of a kind of HFRS is put forward new couple candidate detection method with practical value, is particularly suitable for applying at different medical unit.
Although, some document description some detect the methods of hemorrhagic fever with renal syndrome with golden mark method, when repeating to test according to disclosed method of these documents and program fully, but can't obtain gratifying result, even can't detect at all." Chinese medicine check magazine) " March the 21st in 1998 volume the 2nd interim, disclose a kind of golden method and detected the method for hemorrhagic fever with renal syndrome serological specificity IgM, but fully but can't the repeated experiments result according to this method.Its reason may be that the preparation method of antigen has problem.
At present, when the exploitation hemorrhagic fever with renal syndrome is dripped golden method detection and kit, because the time that IgM antibody produces the earliest, so detect on the IgM for early detection all concentrates on notice.Any droplet golden method and kit at IgG do not disclosed.
Therefore, press for the method and the kit of quick, the correct and easy diagnosis hemorrhagic fever with renal syndrome of a kind of energy.
Purpose of the present invention just provides a kind of method and relevant detection kit of quick, correct and easy detection hemorrhagic fever with renal syndrome.
On the one hand, the invention provides the method for the IgG antibody that whether contains anti-hantavirus in a kind of test sample, it comprises step:
(a) the Hantaan virus nucleoprotein with purifying is coated on the nitrocellulose filter, is formed on the cellulose nitrate that is coated with Hantaan virus nucleoprotein in the point sample district and detects film;
(b) be raw material with inhuman anti-human IgG antibody and collaurum, preparation collaurum-anti-human IgG antibody's bond;
(c) blood serum sample to be detected is dripped in the point sample district of the detection film that obtains in step (a);
(d) the point sample district of the detection film of washing step (c);
(e) bond with collaurum-anti-human IgG antibody of step (b) drips in the point sample district;
(f) the point sample district of the detection film of washing step (e);
The IgG antibody that punctation represents to exist in the sample anti-hantavirus appears in the point sample district, the IgG antibody that punctation represents not exist in the sample anti-hantavirus do not occur.
On the other hand, the invention provides a kind of detection kit that detects hemorrhagic fever with renal syndrome, this kit comprises: nitrocellulose filter, be coated with the Hantaan virus antigen of purifying in the point sample district of this film,
Collaurum-inhuman anti-IgG antibody conjugates solution,
With optional lavation buffer solution.
The present invention also provides the method for the IgM antibody that whether contains anti-hantavirus in a kind of test sample, and it comprises step:
(a) Hantaan virus of purifying is antigen coated on nitrocellulose filter, be formed on the cellulose nitrate that is coated with Hantaan virus nucleoprotein in the point sample district and detect film, wherein the antigen of this purifying is prepared as follows: the inoculation of collect cultivating the host cell of Hantaan virus, through 2800-3200rpm 20-40 minute thick centrifugal after, get supernatant at the centrifugal 1-4 of 35000-45000rpm hour, abandoning supernatant, precipitation is the Hantaan virus antigen of purifying;
(b) be raw material with inhuman anti-human IgM antibody and collaurum, the bond of preparation collaurum-anti-human IgM antibody;
(c) blood serum sample to be detected is dripped in the point sample district of the detection film that obtains in step (a);
(d) the point sample district of the detection film of washing step (c);
(e) bond with collaurum-anti-human IgM antibody of step (b) drips in the point sample district;
(f) the point sample district of the detection film of washing step (e);
The IgM antibody that punctation represents to exist in the sample anti-hantavirus appears in the point sample district, the IgM antibody that punctation represents not exist in the sample anti-hantavirus do not occur.
The present invention is based on and followingly unexpected finds to finish on the basis: after being subjected to Hantaan virus and infecting, IgG can very fast generation, thereby can be used as detected object.As everyone knows, after being infected, IgM antibody produces the earliest usually.The IgG production of antibodies is then slower, and the titre of IgG is lower, therefore is not suitable for usually as the detected object that drips golden method.Yet the present inventor finds, after being subjected to Hantaan virus and infecting, IgG can very fast generation (in about 1-2 days), and the titre level can reach the detection sensitivity of a golden method.And finished the present invention on this basis.
No matter be to select IgG or IgM, detect that the preparation of Hantaan virus antigen is very crucial in order successfully to drip golden method as detected object.Usually require the purity height, combine special antigen with IgG and IgM, in one embodiment of the invention, adopt the substep centrifugation method to prepare antigen, the inoculation of promptly collect cultivating the host cell of Hantaan virus, through 2800-3200rpm 20-40 minute thick centrifugal after, get supernatant at the centrifugal 1-4 of 35000-45000rpm hour, abandoning supernatant, precipitation is the Hantaan virus antigen (nucleoprotein) of purifying.
In order to obtain Hantaan virus, it can be inoculated in the host cell of some employings, as Vero-E6 cell and gerbil jird nephrocyte.Preferably, can be inoculated in the gerbil jird nephrocyte.
After the inoculation, after 35-37 ℃ conduction is cultivated 7-10 days according to a conventional method, can gather in the crops virus.Cultural method can be referring to for example: " modern microbiology experimental technique and application thereof) ", People's Health Publisher, in October, 1997; " medical virology and experimental technique " nineteen ninety, Science Press; " medical experiment virology " People's Medical Officer Press, document such as grade in 1985.
After having obtained the Hantaan virus antigen of purifying, available conventional method is coated on it in point sample district of detecting film.
In order to eliminate non-specific binding, the cellulose nitrate that is coated with Hantaan virus nucleoprotein in the point sample district of acquisition detects film and also handles through pre-combination, for example with 0.75-1.5%BSA solution sealing 2-24 hour.Also can further prolong off-period.But common 2 hours just enough.
In the present invention, operational two resisting with the IgG antibodies can be any inhuman anti-human IgG antibody, and they comprise (but being not limited to): the goat anti-human igg antibody, and mouse anti human IgG antibody and rabbit anti-human igg's antibody are the goat anti-human igg antibodies preferably,
Above-mentioned inhuman anti-human IgM antibody can prepare with conventional method, or purchase obtains;
In the present invention, operational detection film can be the various material films of average pore size between the 0.3-1.2 micron.Preferably, used is nitrocellulose filter.Therefore the average pore size difference of the film of all size, when preparation detects film, should determine the grain size (particle diameter) of collaurum according to pore size.This can be by the weight ratio of sodium citrate and water in the control Preparation of Colloidal Gold process.Studies show that the sodium citrate consumption increases, the collaurum particle diameter descends.Concrete consumption can be determined by normal experiment.For example when the average pore size of this nitrocellulose filter is 0.65 micron, with the preparation of citric acid reducing process and in the preparation the weight ratio of sodium citrate and water be 0.035-0.045: 100,0.038-0.042 preferably: 100, about best 0.04: 100.Can infiltrate through effectively with the prepared collaurum of such collaurum-anti-people IgM or IgG antibody conjugates in 0.65 micron the film.
Usually, prepare the weight ratio 0.0075-0.03 of gold chloride and water in the conventional method of collaurum in the citric acid reducing process: 100, preferably about 0.01-0.02: 100.
After having obtained collaurum and anti-human IgG antibody (or anti-human IgM antibody), both couplings can be carried out according to conventional methods.For example following being prepared: with 10 milliliters of colloidal gold solutions is benchmark, the inhuman anti-human IgG antibody who adds the 8-30 microgram, add BSA after leaving standstill, making the BSA final concentration is 1%, after leaving standstill again, add polyglycol (PEG) 0.8-0.12 gram (preventing collaurum autohemagglutination precipitation), mixing adds an amount of antiseptic.Make collaurum-anti-human IgG antibody (or anti-human IgM antibody) bond solution.Wherein, during coupling, anti-human IgG antibody's (or anti-human IgM antibody) the consumption upper limit also can further improve, though do like this testing result do not had influence, but for reducing the cost, consumption is preferably 8-30 microgram/10 milliliter colloidal gold solution, preferably 9-20 milligram/10 milliliter colloidal gold solution, more preferably 10-15 milligram/10 milliliter colloidal gold solution.
When detecting, generally include following steps:
A, got bag by Hantaan virus detection of antigens film, below pad disposable absorbent material.
B, in the point sample district, drip an amount of (common 1 promptly enough) lavation buffer solution (as PBS-Tween 20 damping fluids), with activation film surface.Wait to infiltrate and get final product (this activation step can omit).
C, drip an amount of blood serum sample, wait to infiltrate and get final product in the point sample district.
D, drip an amount of lavation buffer solution, with washing point sample district in the point sample district.But repeated washing 2-5 time, totally 3 times usually.
E, drip an amount of (common 1 gets final product) collaurum of the present invention-goat anti-human igg antibody's bond (or collaurum-goat anti-human igg antibody's bond solution) in the point sample district.
F, drip an amount of lavation buffer solution, with washing point sample district in the point sample district.But repeated washing 2-5 time, totally 3 times usually.Observations, it is positive punctation to occur in the point sample district, and the redfree spot is negative.
In order to detect more accurately, can to detect IgM simultaneously and/or use the positive and negative control.In kit, also can comprise the material that is selected from down group: collaurum-inhuman anti-IgM antibody conjugates solution, the positive and negative control.
The Hantaan virus antigen of the inventive method preparation is suitable as the antigen protein that golden mark method detects hemorrhagic fever with renal syndrome very much.The gold mark detection test paper of selecting for use this nucleoprotein to make, when in test sample, whether having the antibody of anti-hantavirus, sensitivity improves greatly, can be fast and correctly draw detected object and whether infected by Hantaan virus, thus very help reducing the mortality ratio of hemorrhagic fever with renal syndrome.
In addition, because the specificity of detected IgG antibody is far above IgM antibody, thus can reduce false positive effectively, and help the clinical definite hemorrhagic fever with renal syndrome.
In addition, detection method of the present invention is easy, uses extremely simply, does not need special training just can grasp, therefore be fit to very much detect hemorrhagic fever with renal syndrome this in economy than under-developed area (especially half clearage, land, town and country) incidence of disease disease with high.
Further set forth the present invention below in conjunction with embodiment, these embodiment only are used for illustration purpose and are not used in the qualification scope of the invention.
Embodiment 1
The preparation of Hantaan virus antigen
76-118 is inoculated in the gerbil jird nephrocyte with the strain of hantaan type Hantaan virus, conducts cultivation after 7-10 days, the collecting cell culture at 35-37 ℃ according to a conventional method.
After the ultrasound wave broken cell is handled, in thick centrifugal 30 minutes of 3000rpm, to remove impurity such as cell membrane fragments.Get supernatant, centrifugal 2 hours of 40000rpm so that make Hantaan virus antigen precipitation.Abandoning supernatant is got precipitation.Behind the resolution of precipitate, measure protein content in the solution.Lower as protein content, can centrifugally again concentrate, the final concentration that makes Hantaan virus antigen is 1-2MG/ML.
Embodiment 2
The preparation of collaurum
In 250 milliliters of Erlenmeyer flasks, add 100 milliliters of deionization distilled waters, add 4 milliliters of 1% sodium citrates, boil the back and add 1 milliliter of 1% gold chloride rapidly.Continued to boil 15 minutes, the cooling back transfers to 8.5 with 0.1M sal tartari with pH.
Embodiment 3
The preparation of collaurum-goat-anti IgG antibody conjugates
(a) preparation of goat-anti IgG antibody will be available from the goat-anti IgG antibody of institute of biological products, Shanghai City, and is standby after the ammonium sulfate purification process.
(b) therefore stability test, in order to improve repeatability, should continue stability test to each batch antibody, to determine best antibody addition because there is fluctuation in (or with conventional method preparation) tiring of various biological products of buying.
The goat-anti IgG antibody-solutions that is about 1 mg/ml with a collection of antibody concentration is an example.In the colloidal gold solution of 1 milliliter of preparation in embodiment 2, add 0 microlitre, 2 microlitres, 4 microlitres, 6 microlitres, 8 microlitres, 10 microlitres, 12 microlitres respectively, after at room temperature mixing, at room temperature left standstill 10 minutes.In every pipe, add 0.1 milliliter in 10% sodium chloride, after mixing, at room temperature left standstill 2 hours.Keep the pipe that redness is constant and addition is minimum (in order to save cost, cutting the waste), be and stablize 1 milliliter of optimum protein amount that colloidal gold solution is required.Usually, can on the basis of this optimum protein amount, add 10% again, the actual amount of the antibody that serves as a mark.Found that, when adding people's 6 microlitres, can produce redness, and red the variation not quite.When adding 8 microlitres and 10 microlitres, the redness of generation remains unchanged.Therefore, the actual addition of this batch antibody is decided to be 11 microlitres.
(c) preparation collaurum-goat-anti IgG antibody conjugates solution
Get prepared fresh colloidal gold solution among 10 milliliters of embodiment 2, add 11 microlitre goat anti-human igg antibodies, add 5%BSA after 10 minutes, making the BSA final concentration is 1%.After leaving standstill 10 minutes under the room temperature, add polyglycol (PEG) 0.1 gram (preventing collaurum autohemagglutination precipitation), mixing adds 10% sodium azide (NaN
3) 75 microlitres (playing antisepsis).
Embodiment 4
Detect the preparation of film
1. 0.65 micron nitrocellulose filter is divided into the little lattice of 1 centimetre of 1 cm x with refined lead.
2. drip Hantaan virus antigen (nucleoprotein) solution among the 1 microlitre embodiment 1 in the central authorities (point sample district) of every little lattice.
3. film after the drying, with 1%BSA sealing 2 hours, so that remove non-specific binding, thereby further reduces the false positive probability at room temperature.
Embodiment 5
The preparation of collaurum-goat-anti IgM antibody conjugates
Repeat embodiment 3, difference only is, replaces goat-anti IgG antibody with goat-anti IgM antibody (available from the institute of biological products, Shanghai City).
Embodiment 6
The detection of sample
A, get among the embodiment 4 the detection film of preparation, below pad disposable absorbent material.
B, in the point sample district, drip 1 of PBS-Tween 20 damping fluid, with activation film surface.Wait to infiltrate and get final product.
C, drip 10 microlitre blood serum samples in the point sample district.Wait to infiltrate and get final product.
D, drip 1 of PBS-Tween 20 damping fluid in the point sample district, with washing point sample district.Repeated washing 2 times.
Collaurum-goat anti-human igg antibody's bond (or collaurum-goat anti-human igg antibody's bond solution of preparation among the embodiment 3) 1-2 of E, preparation in point sample district dropping embodiment 3 drips.
F, drip 1 of PBS-Tween 20 damping fluid in the point sample district, with washing point sample district.Repeated washing 2 times.
Observations, it is positive punctation to occur in the point sample district, and the redfree spot is negative.
By the blood serum sample (30 samples and 20 samples respectively) to 2 groups of ELISA method test positive, the detection of 20 routine negative samples and 15 routine normal person's samples can promptly obtain testing result in 3-8 minute.For 50 routine positive, there are 46 routine test positive (this is to be lower than the result that the ELISA method is caused slightly because drip the sensitivity of golden method) and all feminine genders or normal specimens all negative.Because be subjected to Hantaan virus to infect corresponding IgM and the just rising rapidly (reaching or substantially exceed the detection sensitivity of dripping golden method) of IgG antibody in the interior sufferer's body of very short time of back, therefore the accuracy when actual clinical detects estimates will be higher.
Embodiment 7-8
Repeat embodiment 6, difference only is: omit step B among the embodiment 7; Replace PBS-Tween 20 damping fluids with distilled water among the embodiment 8.
As a result, testing result there is not influence.
Embodiment 9
The preparation of kit
The small pieces that the detection film of embodiment 4 preparation are divided into 1 centimetre of 1 cm x (have a point sample district, detect IgG or IgM), or the small pieces of 12 centimetres of cm x (have 2 point sample districts, on a slice, detect IgG and IgM), or the small pieces of 22 centimetres of cm x (have 4 point sample districts, on a slice, detect IgG and IgM and have the positive and negative control).
(the point sample district up) is contained in respectively in the plastic casing of corresponding size with each small pieces film, has the about 0.6 centimetre aperture of diameter above the point sample district, is encased inside the disposable absorbent material below film.
In each plastic casing, place collaurum-goat anti-human igg antibody's bond solution of preparation among collaurum-goat anti-human igg antibody's bond of preparation among the embodiment 3 of 0.5 milliliter of every pipe and/or the embodiment 3 again.
In addition, kit can be equipped with cleansing solution simultaneously, as PBS-Tween 20 damping fluids.
Claims (10)
1. whether contain the method for the IgG antibody of anti-hantavirus in the test sample, it is characterized in that it comprises step:
(a) the Hantaan virus nucleoprotein with purifying is coated on the nitrocellulose filter, is formed on the cellulose nitrate that is coated with Hantaan virus nucleoprotein in the point sample district and detects film;
(b) be raw material with inhuman anti-human IgG antibody and collaurum, preparation collaurum-anti-human IgG antibody's bond;
(c) blood serum sample to be detected is dripped in the point sample district of the detection film that obtains in step (a);
(d) the point sample district of the detection film of washing step (c);
(e) bond with collaurum-anti-human IgG antibody of step (b) drips in the point sample district;
(f) the point sample district of the detection film of washing step (e);
The IgG antibody that punctation represents to exist in the sample anti-hantavirus appears in the point sample district, the IgG antibody that punctation represents not exist in the sample anti-hantavirus do not occur.
2. the method for claim 1, it is characterized in that, the Hantaan virus nucleoprotein of this purifying is prepared as follows: the inoculation of collect cultivating the host cell of Hantaan virus, through 2800-3200rpm 20-40 minute thick centrifugal after, get supernatant at the centrifugal 1-4 of 35000-45000rpm hour, abandoning supernatant, precipitation is the Hantaan virus nucleoprotein of purifying.
3. method as claimed in claim 2 is characterized in that, this host cell is selected from down group: gerbil jird nephrocyte and Vero-E6 cell.
4. the method for claim 1 is characterized in that, this inhuman anti-human IgG antibody is selected from down group: goat anti-human igg antibody, mouse anti human IgG antibody, mouse anti human IgG antibody and rabbit anti-human igg's antibody.
5. the method for claim 1, it is characterized in that, the average pore size of this nitrocellulose filter is 0.65 micron, and this collaurum be with citric acid reducing process preparation and in the preparation the weight ratio of sodium citrate and water be 0.035-0.045: 100, and the weight ratio 0.0075-0.03 of gold chloride and water: 100.
6. the method for claim 1 is characterized in that, the cellulose nitrate that is coated with Hantaan virus nucleoprotein in the point sample district that obtains in the step (a) detects film and also passes through following processing: with 0.75-1.5%BSA solution sealing 2-24 hour.
7. method according to claim 1, it is held to levy and is, in the step (b), collaurum-anti-human IgG antibody's bond is following being prepared: with 10 milliliters of colloidal gold solutions is benchmark, adds the inhuman anti-human IgG antibody of 8-30 microgram, add BSA after leaving standstill, making the BSA final concentration is 1%, after leaving standstill, adds polyglycol 0.8-0.12 gram again, mixing adds an amount of antiseptic.
8. a detection kit that detects hemorrhagic fever with renal syndrome is characterized in that, this kit comprises: nitrocellulose filter, be coated with the Hantaan virus antigen of purifying in the point sample district of this film,
Collaurum-inhuman anti-IgG antibody conjugates solution,
With optional lavation buffer solution.
9. kit as claimed in claim 8 is characterized in that, also comprises the material that is selected from down group: collaurum-inhuman anti-IgM antibody conjugates solution, the positive and negative control.
10. as claim 8 and 9 described kits, it is characterized in that, the average pore size of nitrocellulose filter is 0.65 micron, this inhuman anti-IgG is anti-originally to be the goat anti-human igg antibody, and this Hantaan virus antigen is following obtains: collect the inoculation of cultivating the host cell of Hantaan virus, through 2800-3200rpm 20-40 minute thick centrifugal after, get supernatant at the centrifugal 1-4 of 35000-45000rpm hour, abandoning supernatant, precipitation is the Hantaan virus nucleoprotein of purifying, and making final concentration after the dissolving is 1 mg/ml.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99113544 CN1095545C (en) | 1999-03-18 | 1999-03-18 | Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99113544 CN1095545C (en) | 1999-03-18 | 1999-03-18 | Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever |
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| CN1267831A true CN1267831A (en) | 2000-09-27 |
| CN1095545C CN1095545C (en) | 2002-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 99113544 Expired - Fee Related CN1095545C (en) | 1999-03-18 | 1999-03-18 | Detection method and detection reagent kit for nephrotic syndrome and hemorrhagic fever |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101140258B (en) * | 2007-10-18 | 2010-07-14 | 北京化工大学 | Glucose oxidase film with nitrate cellulose film as substrate and method of producing the same |
| CN1650013B (en) * | 2002-03-22 | 2011-12-21 | 美国传染性疾病军医研究所 | DNA vaccines against hantavirus infections |
| CN1800854B (en) * | 2005-09-05 | 2012-04-25 | 福建省疾病预防控制中心 | Gold-labeled test paper strip for quick diagnosis of hemorrhagic fever with renal syndrome |
| CN103033616A (en) * | 2012-12-24 | 2013-04-10 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN105785000A (en) * | 2016-04-21 | 2016-07-20 | 卢连伟 | Kit for rapidly detecting EHFV-IgM antibody |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100354629C (en) * | 2005-10-17 | 2007-12-12 | 中国人民解放军第三军医大学第一附属医院 | Protein chip/microarray detection system and detection method by using colloidal gold labeled clq as indicator |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1650013B (en) * | 2002-03-22 | 2011-12-21 | 美国传染性疾病军医研究所 | DNA vaccines against hantavirus infections |
| CN1800854B (en) * | 2005-09-05 | 2012-04-25 | 福建省疾病预防控制中心 | Gold-labeled test paper strip for quick diagnosis of hemorrhagic fever with renal syndrome |
| CN101140258B (en) * | 2007-10-18 | 2010-07-14 | 北京化工大学 | Glucose oxidase film with nitrate cellulose film as substrate and method of producing the same |
| CN103033616A (en) * | 2012-12-24 | 2013-04-10 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN103033616B (en) * | 2012-12-24 | 2015-02-11 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN105785000A (en) * | 2016-04-21 | 2016-07-20 | 卢连伟 | Kit for rapidly detecting EHFV-IgM antibody |
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| CN1095545C (en) | 2002-12-04 |
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