CN1266469C - Quick detecting method for formaldehyde in fresh milk - Google Patents
Quick detecting method for formaldehyde in fresh milk Download PDFInfo
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- CN1266469C CN1266469C CN 200410073116 CN200410073116A CN1266469C CN 1266469 C CN1266469 C CN 1266469C CN 200410073116 CN200410073116 CN 200410073116 CN 200410073116 A CN200410073116 A CN 200410073116A CN 1266469 C CN1266469 C CN 1266469C
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Abstract
The present invention relates to a method for quickly detecting formaldehyde in fresh milk. In the method, 2.0 to 8.0 mL of milk sample to be measured is sucked in a blockage colorimetric tube, 0.5 to 5 ml of AgNO3 solution with the concentration of 0.005 to 0.02 mol/L is added, and after the solution is shaken up, 1.0 to 10.0 ml of NaOH solution with the concentration of 0.01 to 0.5 mol/L is added; after the solution is uniformly mixed, a tube plug is opened, and the tube is positioned in a boiling water beaker and is kept for 0.5 to 5 minutes; the beaker is taken off and the change of the color in the tube is observed; if coffees with different depths occur, formaldehyde is mixed in the sample; if jasmine is displayed, formaldehyde is negative. In the present invention, according to the principle that AgNO3 can be reduced into metallic silver by the formaldehyde in alkaline environment, the AgNO3 solution and the NaOH solution are added in the fresh milk, and whether the formaldehyde is contained in the sample is judged through different coloration. The present invention has the advantages of fast detection, obvious coloration phenomenon, high sensitivity, easily grasped positive result and easy popularization and application; the present invention is suitable for being used outdoors and being used for culture, purchase or selling on the spot, and detects that the minimum detection limit of the formaldehyde in the fresh milk can reach 7.6 mu g /mL of milk.
Description
Technical field
The present invention relates to a kind of detection method of formaldehyde, the detection method of formaldehyde in particularly a kind of fresh milk.
Background technology
It is a lot of to can be used for measuring in the fresh milk method of formaldehyde at present, comprising instrument analytical methods such as spectrophotometric and polarography (Wang Jing etc. food security Fast Detection Technique [M] Beijing. Chemical Industry Press, 2002.), this type of analytical approach is mainly used in the mensuration of PARA FORMALDEHYDE PRILLS(91,95) in the laboratory, and generally will distill pre-treatments such as extraction to sample; Chemical analysis method (Dong Wenbin. the identification of adulterated food and check [M]. Xi'an. publishing house of Northwest University, 1996.) reaction with nitric acid and sulfuric acid then arranged, with the reaction of ferric trichloride and with reaction of ferric trichloride and sulfuric acid etc.Be used for fresh milk system formaldehyde detection method and be mainly chemical analysis: with the reaction of nitric acid and sulfuric acid: get detected milk 5ml, carefully add the concentrated sulphuric acid that 2ml contains nitric acid, purple ring person occurs for the formaldehyde positive at two liquid junction boundaries along tube wall.It is faint yellow that negative patient is, be reflected at be viewed as in the 10min good.Reaction with ferric trichloride: get detected milk 1ml and put into test tube, add 0.5ml ferric trichloride hydrochloric acid solution (0.3% ferric trichloride concentrated hydrochloric acid solution), put boiling water bath heating 1min, this moment, cow's milk solidified, and had formaldehyde to exist and purple occurred.Reaction with ferric trichloride and sulfuric acid: get detected milk 10ml in test tube, add 5ml ferric trichloride sulfuric acid solution (adding 1 of 10% ferric trichloride in the 5ml concentrated sulphuric acid), make into two liquid layers, it is positive that the interface is purple.Be used for non-milk system (medium) formaldehyde as food and drink and air assay method (permitted tree peony etc. foodsafety and analyzing and testing [M]. Beijing. Chemical Industry Press, 2003) have: (1) gets 5ml sample preparation liquid in test tube, add a small amount of 4% phenylhydrazine hydrochloride, the 5% iron nitrosyl sodium cyanide solution that adds 4 new preparations, and 12 10% potassium hydroxide solutions, be blueness or pewter if there is formaldehyde to exist.(2) get 1ml sample preparation liquid in test tube, add 2ml72% sulfuric acid and mix, add the small amount of solid chromotropic acid, test tube is put into 60 ℃ of water-baths and is heated 10min, and being distinct aubergine is the formaldehyde positive.(3) get 10ml sample preparation liquid, add 2ml0.1% phloroglucin and several potassium hydroxide solutions, heated and boiled is half an hour approximately, and taking on a red color is the formaldehyde positive.(4) (Lee builds to have periodical report iron-phenanthroline development process to can be used for detecting free formaldehyde in the fabric in addition, Xu Yongjun etc. iron-phenanthroline indirect spectrophotometric methods are measured free formaldehyde [J] in the fabric. analytical chemistry, 2000,28 (2): 261): be cooled to room temperature after the boiling water heating behind adding silver nitrate and the NaOH mixing in the test sample, add the 5mL0.004mol/L ammonium ferric sulfate successively, color is salmon pink person for the formaldehyde positive behind 5mlpH4.5NaAc-HAc damping fluid and 3ml0.1% phenanthroline (joining with stylish) the vibration mixing.
The method of above-mentioned many mensuration formaldehyde of mentioning all is unwell to the detection of formaldehyde in the fresh milk, and does not more occur emerging according to the commercially available reagent box that said method is made.And the fresh milk system is all not ideal also to be not suitable for being made into kit and to apply because these detection methods are used for.Instrument analytical method needs sample is carried out various pre-treatments, and color condition is wayward, and phenomenon is difficult to be held, sensitivity not high and scene of being not easy to and outdoor operation.The chemical analysis rule uses that reagent has has severe corrosive and certain toxicity, and the use of reagent, keeping are all inconvenient, and the harsh formality of the color condition that has is loaded down with trivial details, and sensitivity is too low, and is all undesirable for the detection effect of formaldehyde in the fresh milk.
Summary of the invention
The object of the present invention is to provide a kind of detection quick, the colour generation phenomenon is obvious, highly sensitive, positive findings is easy to hold, and be adapted at outdoor and the on-the-spot use of various breed, purchase or sale, be easy to apply, and detect the detection method of formaldehyde in the fresh milk that the accessible minimum detectability of formaldehyde in the fresh milk is 7.6 μ g/mL breasts.
For achieving the above object, the technical solution used in the present invention is: at first draw milk sample 2.0～8.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.005～0.02mol/L
3Solution 0.5～5ml, the NaOH solution 1.0～10.0ml that adds 0.01～0.5mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.5～5 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Because the present invention is according in alkaline environment, AgNO
3Can be reduced to the principle of argent by formaldehyde, in fresh milk, add AgNO
3With NaOH solution, come whether to contain in the judgement sample formaldehyde by different colour developings.
Embodiment
Embodiment 1: at first draw milk sample 2.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.02mol/L
3Solution 0.5ml, the NaOH solution 10.0ml that adds 0.5mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 5 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Embodiment 2: at first draw milk sample 5.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.005mol/L
3Solution 5.0ml, the NaOH solution 6ml that adds 0.3mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.5 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Embodiment 3: at first draw milk sample 8.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.01mol/L
3Solution 3.0ml, the NaOH solution 2ml that adds 0.1mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 2 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Embodiment 4: at first draw milk sample 4.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.008mol/L
3Solution 1.0ml, the NaOH solution 1ml that adds 0.01mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 4 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Embodiment 5: at first draw milk sample 6.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.015mol/L
3Solution 2.5ml, the NaOH solution 8ml that adds 0.06mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 1 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Embodiment 6: at first draw milk sample 3.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.009mol/L
3Solution 4.0ml, the NaOH solution 5ml that adds 0.4mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.7 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Claims (7)
1, the detection method of formaldehyde in the fresh milk is characterized in that: at first draw milk sample 2.0～8.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.005～0.02mol/L
3Solution 0.5～5ml, the NaOH solution 1.0～10.0ml that adds 0.01～0.5mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.5～5 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
2, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 2.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.02mol/L
3Solution 0.5ml, the NaOH solution 10.0ml that adds 0.5mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 5 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
3, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 5.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.005mol/L
3Solution 5.0ml, the NaOH solution 6ml that adds 0.3mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.5 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
4, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 8.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.0lmol/L
3Solution 3.0ml, the NaOH solution 2ml that adds 0.1mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 2 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
5, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 4.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.008mol/L
3Solution 1.0ml, the NaOH solution 1ml that adds 0.01mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 4 minutes, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
6, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 6.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.015mol/L
3Solution 2.5ml, the NaOH solution 8ml that adds 0.06mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 1 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
7, the detection method of formaldehyde in the fresh milk according to claim 1 is characterized in that: at first draw milk sample 3.0ml to be checked in the color comparison tube with pipe close, add the AgNO of 0.009mol/L
3Solution 4.0ml, the NaOH solution 5ml that adds 0.4mol/L after shaking up again, open color comparison tube behind the vibration mixing with pipe close, place the boiling water beaker to keep 0.7 minute, take off change in color in the beaker observation tube, if the coffee color of different depth occurs, show to be mixed with formaldehyde in the sample, if show the faint yellow formaldehyde feminine gender that is.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410073116 CN1266469C (en) | 2004-09-24 | 2004-09-24 | Quick detecting method for formaldehyde in fresh milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410073116 CN1266469C (en) | 2004-09-24 | 2004-09-24 | Quick detecting method for formaldehyde in fresh milk |
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|---|---|
| CN1588011A CN1588011A (en) | 2005-03-02 |
| CN1266469C true CN1266469C (en) | 2006-07-26 |
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|---|---|---|---|
| CN 200410073116 Expired - Fee Related CN1266469C (en) | 2004-09-24 | 2004-09-24 | Quick detecting method for formaldehyde in fresh milk |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2795470C1 (en) * | 2022-05-12 | 2023-05-03 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный университет" (СПбГУ)" | Method for qualitative and quantitative colorimetric determination of formaldehyde in milk |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110018157A (en) * | 2019-04-01 | 2019-07-16 | 中华人民共和国昆山海关 | A kind of textile formaldehyde method for rapidly testing |
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2004
- 2004-09-24 CN CN 200410073116 patent/CN1266469C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2795470C1 (en) * | 2022-05-12 | 2023-05-03 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный университет" (СПбГУ)" | Method for qualitative and quantitative colorimetric determination of formaldehyde in milk |
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| Publication number | Publication date |
|---|---|
| CN1588011A (en) | 2005-03-02 |
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