CN1265797C - Application of timosaponin A3 for preparing medicine for treating No.2 type diabetes mellitus - Google Patents
Application of timosaponin A3 for preparing medicine for treating No.2 type diabetes mellitus Download PDFInfo
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- CN1265797C CN1265797C CN 02160151 CN02160151A CN1265797C CN 1265797 C CN1265797 C CN 1265797C CN 02160151 CN02160151 CN 02160151 CN 02160151 A CN02160151 A CN 02160151A CN 1265797 C CN1265797 C CN 1265797C
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Abstract
The present invention relates to an application of timosaponin AIII in preparing medicine for reducing blood sugar and treating type II diabetes, wherein the timosaponin is extracted and separated from rhizoma anemarrhenae in traditional Chinese medicine and can reduce blood sugar and treat type II diabetes through the certification of pharmacological experiments.
Description
Technical field
The effective ingredient that the present invention relates to extraction separation from the Chinese medicine Rhizoma Anemarrhenae is effective to the type ii diabetes treatment through the pharmacological testing proof, more specifically refers to the effective ingredient 1-timosaponin A-1 of extraction separation from the Chinese medicine Rhizoma Anemarrhenae
III, it can be used in preparation treatment type ii diabetes medicine.
Background technology
Diabetes are that concentration of glucose raises in the blood owing to insulin in the human body definitely or relatively lacks a kind of common clinical that causes, and then sugar discharges from urine in a large number, and polydipsia, polyuria, polyphagia occur, become thin, symptom such as dizzy, weak.Further develop and then cause the various serious acute and chronic complication of whole body, threaten healthy.Wherein the type i diabetes patient loses the function that produces insulin fully, relies on insulinize and needs lifelong the use; Insulin is in relative deficiency state in the type ii diabetes patient body, needn't rely on insulin fully and can treat with other medicines.In diabetics, type ii diabetes has accounted for great majority.Diabetes are the commonly encountered diseases in the modern society, high morbidity, serious threat human beings'health and life.
At present the main method of western medical treatment diabetes is insulinize and hypoglycemic drugs such as oral sulphanylureas, biguanides, but insulinize costs an arm and a leg, and other types of drug or curative effect are limited, or toxic and side effects is obvious.
In recent years, studies show that in a large number, Chinese medicine demonstrates very big advantage in the clinical treatment of type ii diabetes, but the existing most effective ingredient of Chinese patent medicine is unclear, the mechanism of action is not clear, dosage form falls behind, and urgency waits to develop effective ingredient and the mechanism of action is clear, stable and controllable for quality, dosage form is advanced, take Chinese medicine preparation easy to carry.
Chinese medicine Rhizoma Anemarrhenae clearing away heat-fire promotes the production of body fluid and moisturizes, and mainly has effects such as antibiotic, antiviral, analgesic, blood sugar lowering, but does not see the effective site of its treatment type ii diabetes and the report of effective ingredient.1-timosaponin A-1
IIIThe pharmacological action of (Sarsasapogenin-3-0-β-D-Glucopyranose. (→ 2)-β-D-galactopyranoside) monomer component does not appear in the newspapers yet.
Summary of the invention
The present invention seeks to overcome the weak point that conventional Chinese medicine exists, adopt chemical method from the Chinese medicine Rhizoma Anemarrhenae, to isolate effective ingredient---1-timosaponin A-1
III,, this chemical compound is used in the medicine of preparation treatment type ii diabetes through pharmacological testing.
The present invention adopts alcohol reflux 3 times of Chinese medicine rhizoma ane marrhenae, and filtrate decompression is reclaimed, and makes Rhizoma Anemarrhenae extractum, with the low amounts of water dissolving, use the water saturation n-butanol extraction again, n-butanol layer is mixed silicagel column on the sample with methanol, with n-butyl alcohol-chloroform (1: 1) eluting, separate obtaining the pure compound 1-timosaponin A-1
IIIStructural formula is as follows:
Its molecular formula is C
39H
64O
13, molecular weight is 740, chemistry Sarsasapogenin by name-3-O-β-D-glucopyranosyl (1 → 2)-β-D-galactopyranoside.
The present invention adopts said method to extract the 1-timosaponin A-1 that obtains
IIIAt first carry out the conventional test of pesticide effectiveness.
Male KKAy mice (spontaneous type diabetic mice, the good animal model of type ii diabetes) is measured at random and fasting glucose, according to measurement result mice is divided into 3 groups.
1, blank group: normal feeding;
2, positive controls: orally give rosiglitazone (ROS, GlaxoSmithKline PLC (Tianjin) company, lot number: 02060041.Rosiglitazone is the thiazolidinediones euglycemic agent, and dosage is little, and is evident in efficacy, can increase the cell energy consumption and improve glucose metabolism, is the blood sugar lowering more widely of domestic and international clinical use at present);
3, administration group: orally give medicine (1-timosaponin A-1 of the present invention
III, HHG-001).
Once a day, successive administration 23 days is before administration, administration 13 days, administration adopt the fasting glucose of glucose assays kit measurement KKAy mice in the time of 23 days.The mensuration of fasting glucose mainly is to be used for weighing basic blood sugar level, and the fasting glucose height illustrates this mouse blood sugar height, is one of symptom of diabetes.The result shows that medicine of the present invention can significantly reduce the fasting glucose of KKAy diabetic mice.
Carry out glucose tolerance test after the administration end, measure to before the sugar and to the blood glucose value after the sugar.Glucose tolerance is to weigh one of glycometabolic leading indicator.Blood glucose can return to basic value in the certain hour of normal mouse feed back, and ill Mus blood glucose in the identical time can not return to basic value.The result shows that medicine of the present invention can significantly reduce the KKAy diabetic mice to before sugared and to the blood glucose value after the sugar, obviously improves its glucose load.
When finishing, administration carries out insulin tolerance test.Insulin tolerance also is to weigh glycometabolic index.Ill Mus generally has insulin resistance, i.e. the effect deficiency of insulin normal physiological effect, and especially muscle, fatty tissue are to impel complication such as hypertension, hyperlipidemia that the major reason of development takes place to the obstacle that utilizes of glucose to show as peripheral tissues.If medicine is effective, the sensitivity to insulin after the ill Mus administration improves, thereby promotes blood glucose to descend.The result shows that medicine of the present invention can improve the sensitivity of KKAy diabetic mice to insulin, promotes blood glucose to descend.
Measure the mice body weight before and after the administration respectively.Under the normal condition, after the administration, the mice body weight significant change should not occur, if weight loss illustrates that mice body constitution descends; And body weight rises, even mouse blood sugar descends, illustrates that also this medicine has the side effect of weight increase.Experimental result shows that medicine of the present invention does not have obvious influence to the body weight of KKAy diabetic mice, and positive drug ROS obviously increases the mice body weight.
When finishing, administration measures the mice blood fat.Diabetics in hyperglycemia often with hyperlipidemia.Measuring blood fat mainly is to observe whether this medicine also has blood fat reducing in the hypoglycemic while effect.Experimental result shows that medicine of the present invention can significantly reduce the blood fat of KKAy diabetic mice, and positive drug ROS does not have this effect.
The present invention studies have shown that, 1-timosaponin A-1
IIIHave good reduction fasting glucose and blood fat, improve glucose tolerance and, do not influence the effect of weight in patients simultaneously the sensitivity of insulin.
Fasting glucose, blood fat, glucose tolerance, insulin tolerance are the important indicators of weighing type ii diabetes.The mensuration of fasting glucose mainly is to be used for weighing basic blood sugar level, and the fasting glucose height illustrates patient basis blood glucose height, and this is one of symptom of diabetes, in addition, diabetics when hyperglycemia occurring often with hyperlipidemia.Glucose tolerance is to weigh one of glycometabolic index, and the blood glucose of healthy person can return to basic value in the certain hour of back on the feed, and diabetics blood glucose in the identical time can not return to basic value.Insulin tolerance also is to weigh glycometabolic index.Diabetics generally has insulin resistance, it is the effect deficiency that insulin is carried out its normal biological agent, it is muscle, the fatty tissue obstacle that utilizes to glucose to show as the In-particular of peripheral tissues, and this is to impel complication such as hypertension, hyperlipidemia that the major reason of development takes place.By pharmacological testing and presentation of results 1-timosaponin A-1 thereof
IIICan be used as in the preparation treatment diabetes medicament and use.
Description of drawings
Fig. 1 be timosaponin to the glucose tolerance of KKAy diabetic mice influence result of the test figure, wherein-◆-expression blank group ,-■-expression positive controls ,-△-expression drug test group compares with the blank group,
*P＜0.01
Fig. 2 be timosaponin to KKAy diabetic mice insulin tolerance influence result of the test figure, wherein-◆-expression blank group ,-■-expression positive controls ,-△-expression drug test group compares with the blank group,
*P＜0.01
Beneficial effect:
1, the present invention has excavated the known compound timosaponin as the new purposes in the preparation treatment type ii diabetes medicine, has opened up new application.
2, timosaponin of the present invention not only has significant blood sugar lowering, blood fat, improves the pharmacological effect of glucose tolerance and insulin tolerance, and body weight is not had obvious influence, and side effect is little.
The specific embodiment
The present invention is further elaborated below in conjunction with specific embodiment, but it is not had any restriction.
Embodiment 1 medicine of the present invention is to the influence test of KKAy diabetic mice fasting glucose
15 male KKAy mices are raised in the laminar flow cabinet of cleaning level Animal House, measure at random and fasting glucose after adapting to for 2 weeks, according to measurement result 15 mices are divided into 3 groups, every group 5, difference normal feeding (blank group), orally give 4mg/kg rosiglitazone (positive controls, ROS) and 400mg/kg medicine of the present invention (administration group).Once a day, successive administration 23 days is before administration, administration 13 days, administration adopt the fasting glucose of glucose assays kit measurement mice in the time of 23 days.The mensuration of fasting glucose mainly is to be used for weighing basic blood sugar level, and the fasting glucose height illustrates this mouse blood sugar height, is one of symptom of diabetes.
The result: medicine of the present invention can significantly reduce the fasting glucose of KKAy diabetic mice, sees Table 1.
Table 1 medicine of the present invention is to the influence test of KKAy diabetic mice fasting glucose
| Group | Fasting glucose | ||
| Before the administration | Administration 13 days | Administration 23 days | |
| Blank group ROS group HHG-001 group | 16.58±4.99 16.58±6.33 17±5.91 | 18.66±3.72 11.24±2.22 ** 12.5±5.52 * | 17.88±1.75 12.06±4.89 * 11.46±.66 ** |
*P＜0.05
*P＜0.01 is compared with blank
Embodiment 2 medicines of the present invention are to the influence test of KKAy diabetic mice glucose tolerance
Grouping and test method are with embodiment 1, and administration is carried out glucose tolerance test after finishing.Orally give 2.5g/kg glucose is measured the blood glucose value that blank group and administration group are given the sugar front and back.Glucose tolerance is to weigh one of glycometabolic index.Blood glucose can return to basic value in the certain hour of normal mouse feed back, and ill Mus blood glucose in the identical time can not return to basic value.
The result: medicine of the present invention can significantly reduce the KKAy diabetic mice to before sugared and to the blood glucose value after the sugar, obviously improves its glucose load, sees Fig. 1.
Embodiment 3 medicines of the present invention are to the influence test of KKAy diabetic mice insulin tolerance
Grouping and test method are carried out insulin tolerance test with embodiment 1 when administration finishes, measure the blood glucose value of subcutaneous injection insulin front and back respectively.Insulin tolerance also is to weigh glycometabolic index.Ill Mus generally has insulin resistance, if medicine is effective, the sensitivity to insulin after the ill Mus administration improves, thereby promotes blood glucose to descend.
The result: medicine of the present invention can improve the sensitivity of KKAy diabetic mice to insulin, promotes blood glucose to descend, and sees Fig. 2.
Embodiment 4 medicines of the present invention are to the influence test of KKAy diabetic mice body weight
Grouping and test method are measured the mice body weight respectively with embodiment 1 before and after administration.Under the normal condition, after the administration, the mice body weight significant change should not occur, if weight loss illustrate that mice body constitution descends, and body weight rises, even mouse blood sugar descends, illustrates that also this medicine has the side effect of weight increase.
The result: medicine of the present invention does not have obvious influence to the body weight of KKAy diabetic mice, and positive drug ROS obviously increases the mice body weight, sees Table 2.
Table 2 medicine of the present invention is to the influence test of KKAy diabetic mice body weight
| Group | Body weight | ||
| Before the administration | After the administration | Value added | |
| Blank group ROS group HHG-001 group | 42.2±3.16 41.0±2.35 40.6±3.44 | 43.8±2.68 48.4±4.28 * 40.4±3.78 | 1.8±0.84 7.4±3.36 -0.2±1.64 |
*Compare before P＜0.05 and the administration
Grouping and test method are measured the mice blood fat with embodiment 1 when administration finishes.The test blood fat mainly is to observe whether this medicine also has blood fat reducing in the hypoglycemic while effect.
The result: medicine of the present invention can significantly reduce the blood fat of KKAy diabetic mice, and positive drug ROS does not have this effect, sees Table 3.
Table 3 medicine of the present invention is to the influence test of KKAy diabetic mice blood fat
| Group | Blood fat |
| Blank group ROS group HHG-001 group | 192.39±33.41 214.98±69.85 120.16±45.97 * |
*P＜0.05 is compared with blank
Claims (2)
1, the 1-timosaponin A-1 that has following structure
IIIApplication in the medicine of preparation treatment type ii diabetes.
2,1-timosaponin A-1 according to claim 1
IIIApplication, it is characterized in that the application in the medicine of preparation blood sugar lowering, blood fat reducing, raising glucose tolerance and insulin tolerance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02160151 CN1265797C (en) | 2002-12-31 | 2002-12-31 | Application of timosaponin A3 for preparing medicine for treating No.2 type diabetes mellitus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02160151 CN1265797C (en) | 2002-12-31 | 2002-12-31 | Application of timosaponin A3 for preparing medicine for treating No.2 type diabetes mellitus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1513463A CN1513463A (en) | 2004-07-21 |
| CN1265797C true CN1265797C (en) | 2006-07-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02160151 Expired - Fee Related CN1265797C (en) | 2002-12-31 | 2002-12-31 | Application of timosaponin A3 for preparing medicine for treating No.2 type diabetes mellitus |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107913283A (en) * | 2016-02-20 | 2018-04-17 | 通化华夏药业有限责任公司 | A kind of preparation method of 1-timosaponin A-1 I |
| CN105663626A (en) * | 2016-03-04 | 2016-06-15 | 广东药学院 | Rhizoma anemarrhenae effective part as well as preparation method and application thereof |
| CN107158016B (en) * | 2017-06-09 | 2019-10-11 | 徐州医科大学 | The application of timosaponin and its aglycon in preparation prevention and treatment early diabetic nephropathy drug |
| CN110025717B (en) * | 2019-05-20 | 2022-02-08 | 上海中医药大学 | Application of timosaponin enzymatic hydrolysate in preparation of medicines for inhibiting skin superficial fungi |
| CN116712445A (en) * | 2023-05-05 | 2023-09-08 | 上海中医药大学 | Timosaponin enzymolysis product and application of timosaponin AIII in preparation of medicines for treating hyperlipidemia |
-
2002
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