CN1264506C - Pharmaceutical combination containing red sage root element and preparation method thereof - Google Patents
Pharmaceutical combination containing red sage root element and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a pharmaceutical composition containing a red sage root element and a preparation method thereof, which is characterized in that the red sage root element is obtained from traditional Chinese medicine red sage root and mixed with pharmaceutical auxiliary materials to realize that the content of red sage root element is not less than 80%, so an injection preparation is prepared. Pharmacological experiments indicate that each preparation of the present invention has good pharmaceutical action.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine pharmacy, be specifically related to a kind of pharmaceutical composition that contains danshensu and preparation method thereof.
Technical background
Radix Salviae Miltiorrhizae (Radix Salviae Miltiorrhiza Bge) is Labiatae (Labiatae) salvia, has another name called: Radix Salviae Miltiorrhizae, Arisaema balansae Engl., Herba Wedeliae Wallichii, Radix Campylotropis Hirtella (Herba Myrsines Africanae) etc.Bitter in the mouth, cold nature.Have functions such as promoting blood flow to regulate menstruation, promoting tissue regeneration by removing blood stasis, tranquillizing and allaying excitement, removing heat from blood eliminating carbuncle, reducing swelling and alleviating pain.Be used for the treatment of diseases such as menoxenia, dysmenorrhea, postpartum stagnation stomachache, joint aches, neurasthenia's insomnia, cardiopalmus, carbuncle sore tumefacting virus.The clinical proof of modern medicine, Radix Salviae Miltiorrhizae have blood vessel dilating and the effect of promoting coronary artery blood flow, are used for treating diseases such as coronary heart disease, angina pectoris, myocardial infarction, tachycardia, and significant curative effect is arranged.The Radix Salviae Miltiorrhizae main component is liposoluble constituent and water soluble ingredient, the chemical work of previous Radix Salviae Miltiorrhizae concentrates on liposoluble constituent, therefrom isolating TANSHINONES is a series of diterpene compounds of representative, confirm to have certain physiologically active through pharmacological evaluation, but a large amount of pharmacology and clinical research result show that the activity of the water soluble ingredient of Radix Salviae Miltiorrhizae is better than liposoluble constituent, red sage root water soluble ingredient is: Radix Salviae Miltiorrhizae acid A, B, C, Radix Salviae Miltiorrhizae acid A claim danshensu again, its structure is D (+)-β-(3, the 4-dihydroxy phenyl) lactic acid, Radix Salviae Miltiorrhizae acid B is formed by the danshensu of 3 molecules and the caffeic acid condensation of 1 molecule, it is exactly salvianolic acid B, this material instability changes into danshensu under certain condition; Radix Salviae Miltiorrhizae acid C is the condensation substance of 2 molecule danshensus; The content 0.02% of danshensu in the red rooted salvia, the content of salvianolic acid B is 3-9%, and the content 5-8% of danshensu in its market extract, the detection of salvianolic acid B is almost feminine gender, above data declaration under certain condition, Radix Salviae Miltiorrhizae total phenolic acids can be converted into danshensu.
Danshensu have a great pharmacological effects.(1) to the effect of cardiovascular system: prove that through pharmacological testing danshensu can significantly increase the coronary flow of the Cavia porcellus of exsomatizing; Diastole isolated pig coronary artery; The blood vessel function that contracts of antagonism morphine and propranolol; Antagonism or remarkable protection are changed by the rat S-T section ischemic electrocardio that pituitrin causes; Obviously dwindle the scope of Cor Canitis muscle infarction and improve left ventricular function; Promote the reparation of ischemia myocardial damage cell; Be the main component that resists myocardial ischemia in the salvia-soluble part; And have effects such as the heart of improvement and periphery microcirculation disturbance.(2) to the effect of blood system: danshensu can suppress the inductive rabbit platelet of ADP and assemble; Platelet adhesion is reduced; Obvious blood viscosity lowering; Suppress hematoblastic synthetic; Antagonism thrombosis and blood coagulation resisting function; And can activate fibrinolytic, promote fibrin (former) degraded; Still have a calcium antagonism, flow in the erythrocytic calcium that can suppress to excite, stablize erythrocyte membrane, thereby produce antihemolysis by potassium chloride.
Patent (application number 01126883) adopts ion exchange resin or purification with macroreticular resin Radix Salviae Miltiorrhizae to obtain danshensu, this method is not done any description to the content of danshensu, just bright in the form this method is feasible, whether can industrialization, industrialization also describes, this invention belongs to the conclusion that the little experiment of laboratory obtains by analysis; Consult patent documentation, do not have a kind of method that content of Danshensu is reached more than 80%.
Summary of the invention
For these reasons, the present invention adopts ultrasonic water extraction to extract, the extracting solution precipitate with ethanol is removed a part of impurity, with supernatant alkalize, acidification, the solution that obtains is carrying out eluting with polar macroporous resin, and eluent obtains content of Danshensu with ethyl acetate extraction and is not less than 80% effective site, is mixed with into ejection preparation with pharmaceutic adjuvant, pharmacological evaluation shows that the preparation of respectively organizing of the present invention has better pharmacological action.
The present invention is achieved through the following technical solutions.
One. process recipes
(1) gets red rooted salvia, chopping is put in the supersound extraction jar, adds 6-10 water doubly and carries out supersound extraction, time is 20-50 minute, frequency of oscillation is 20-100KHz, and the control temperature is a room temperature, extracts 2-4 time, merge extractive liquid, filters, it is 1.05-1.15 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 50%-70%, leaves standstill 24 hours in 0 ℃-10 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 9-11, boiled 0.5-3 hour, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2-3 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with macroporous adsorptive resins on the above-mentioned solution, doubly wash with 3-6 earlier, eluent discards, the sodium chloride eluting of reuse 6-10 times 1-3mol/l, eluent is with 0.5-3 ethyl acetate extraction doubly 3-9 time, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site;
(3) get active component of red sage root 80-100 weight portion, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains aqueous injection;
(4) get active component of red sage root 160-200 weight portion, pharmaceutic adjuvant 200-240 weight portion adds the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains infusion solution;
(5)) get active component of red sage root 80-100 weight portion, pharmaceutic adjuvant 100-120 weight portion adds the dissolving of injection water fully, uses the 0.22um filtering with microporous membrane, and filtrate fill drying obtains injectable powder.
The pharmaceutic adjuvant of infusion solution is a kind of of sodium chloride, glucose.
Excipient is one or more in mannitol, sucrose, the lactose in the preparation of powder injection formulation.
Macroporous adsorbent resin is a polar macroporous adsorption resin.
Two. check and analysis
The check and analysis of active component of red sage root content of Danshensu
1. instrument and reagent high performance liquid chromatograph comprise LC-10ATvp type solvent delivery pump, SPD-10ATvp type UV, visible light detector (day island proper Tianjin company); N2000 chromatographic work station (Zhejiang University's intelligent information Graduate School of Engineering); KQ3200 type ultrasonic washing instrument (Kunshan Ultrasonic Instruments Co., Ltd.), TGL-16G high speed tabletop centrifuge (Anting Scientific Instrument Factory, Shanghai).Chromatographically pure methanol (the effective company of Beijing chemical reagent); Water is tri-distilled water (self-control); Other reagent is analytical pure.Danshensu reference substance (Nat'l Pharmaceutical ﹠ Biological Products Control Institute).
2. chromatographic column tested in chromatographic condition and system suitability: the C of Di Ma company
18Post (5 μ m, 150mm * 4.6mm, I.D); Mobile phase: methanol-5% acetic acid (25: 75); Flow velocity: 1.0ml/min detects wavelength: 280nm.Number of theoretical plate should be not less than 3000 by danshensu peak compute.
3. reference substance solution prepares precision and takes by weighing danshensu reference substance 5.0mg, inserts in the 10ml measuring bottle, adds methanol, ultrasonicly makes dissolving, adds methanol again to scale, shakes up, promptly.
4. standard curve preparation difference precision is drawn above-mentioned reference substance solution 1 μ l, 2 μ l, 4 μ l, 6 μ l, 8 μ l, 10 μ l, 12 μ l, sample introduction is measured under the said determination condition, danshensu is the good linear relation in 0.5 μ g～6.0 μ g scopes as a result, regression equation is Y=9726483X+3989, r=0.9997.
5. the about 10mg of active component of red sage root of the present invention decided in the accurate title of the preparation of need testing solution, put in the tool plug conical flask, the accurate methanol 10ml that adds claims to decide weight, supersound process 30min, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methanol, shake up, filter, get subsequent filtrate centrifugal (12000rpm) 10min, supernatant is as need testing solution.
6. accurate each the 10 μ l of need testing solution that draw of algoscopy inject chromatograph of liquid, measure peak area, calculate content with standard curve.
Above-mentioned experiment is carried out three times.The results are shown in Table 1.
Table 1 active component of red sage root content of Danshensu
| Group | Active component of red sage root content of Danshensu (%) | Average content (%) |
| 1 2 3 | 83.2 82.5 82.9 | 82.9 |
Conclusion: by above check and analysis experiment, we can analyze, adopt that content of Danshensu meets the requirement of national Bureau of Drugs Supervision to Chinese medicine new medicine 5 classes greater than 80% in the active component of red sage root that technology of the present invention obtains, prove absolutely that the present invention has practical significance.
Three. pharmacology embodiment
Embodiment 1
Influence experiment to anoxia in mice endurance
The experiment medicine: the present invention respectively organizes preparation (Qianluchun Science and Technology Co., Ltd., Beijing's laboratory provides)
Radix Salviae Miltiorrhizae Injection (Zhejiang Tianrui pharmaceutcal corporation, Ltd)
Normal saline (Qianluchun Science and Technology Co., Ltd., Beijing's laboratory provides)
Experimental technique: get 50 of healthy Kunming mouses, body weight 20～24g.Every group 10, male and female half and half, sub-cage rearing.With the conversion of people's conventional therapy dosage is the dosage of mice.The conversion formula is: tested animal is tried out the body surface area ratio of the body surface area ratio/known animal of dosage=known animals administer amount * tested animal.Control group administered physiological saline, 1 time/d, continuous 7d.1h after the last administration, it is the 150ml port grinding bottle that mice is placed volume respectively, in put the 15g sodica calx, its time-to-live of airtight observation.The results are shown in Table 2.
The influence of table 2 pair mice normobaric hypoxia (X ± SD)
| Group | Mus number (only) | Mean survival time (min) |
| Normal saline Radix Salviae Miltiorrhizae Injection aqueous injection of the present invention infusion solution of the present invention injectable powder of the present invention | 10 10 10 10 10 | 15.81±2.91 20.12±3.62 ** 31.98±4.68 **[ *] 32.41±4.15 **[ *] 32.06±4.23 **[ *] |
Annotate: * * P＜0.01, compare [*] P＜0.05 with positive controls
Embodiment 2
To the anoxybiotic protective effect experiment of mouse cardiac muscle
The experiment medicine: the present invention respectively organizes preparation (Qianluchun Science and Technology Co., Ltd., Beijing's laboratory provides)
Radix Salviae Miltiorrhizae Injection (Zhejiang Tianrui pharmaceutcal corporation, Ltd)
Normal saline (Qianluchun Science and Technology Co., Ltd., Beijing's laboratory provides)
Experimental technique: get 50 of healthy Kunming mouses, body weight 18～22g is divided into 5 groups at random.Every group of male and female half and half, sub-cage rearing.With the conversion of people's conventional therapy dosage is the dosage of mice.The conversion formula is: tested animal is tried out the body surface area ratio of the body surface area ratio/known animal of dosage=known animals administer amount * tested animal.Control group administered physiological saline.Medication: 1 time/d, continuous 6d.1h is with urethane 1.2g/kg intraperitoneal injection of anesthesia after the last administration, and back fixation is separated trachea, with the bulldog clamp folder pipe of holding one's breath, observes electrocardio with electrocardiogram equipment, and writes down the little mousetrap with stopwatch and hold one's breath time of Guan Houzhi electrocardio disappearance.The results are shown in Table 3.
The anoxybiotic influence of table 3 pair mouse cardiac muscle (X ± SD)
| Group | Mus number (only) | Mean survival time (min) |
| Normal saline Radix Salviae Miltiorrhizae Injection aqueous injection of the present invention infusion solution of the present invention injectable powder of the present invention | 10 10 10 10 10 | 5.01±0.28 9.81±0.77 ** 14.12±1.34 **[ *] 14.01±1.29 **[ *] 14.56±1.38 **[ *] |
Annotate: * * P＜0.01, compare [*] P＜0.05 with positive controls
Conclusion: show that by above pharmacological evaluation the preparation of respectively organizing of the present invention has better pharmacological action.
Four. preparation embodiment
Embodiment 1
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 6 times carries out supersound extraction, time is 20 minutes, frequency of oscillation is 20KHz, and the control temperature is a room temperature, extracts 2 times, merge extractive liquid, filters, it is 1.05 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 50%, leaves standstill 24 hours in 0 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 9, boiled 0.5 hour, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 3 times of washings, eluent discards, the sodium chloride eluting of 6 times of 1mol/l of reuse, eluent is with 0.5 times ethyl acetate extraction 3 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 83.1%)
(3) get active component of red sage root 80 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 160 grams, pharmaceutic adjuvant glucose 240 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 80 grams, pharmaceutic adjuvant mannitol 120 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.
Embodiment 2
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 10 times carries out supersound extraction, time is 50 minutes, frequency of oscillation is 100KHz, and the control temperature is a room temperature, extracts 4 times, merge extractive liquid, filters, it is 1.15 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 70%, leaves standstill 24 hours in 10 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 11, boiled 3 hours, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 3 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 6 times of washings, eluent discards, the sodium chloride eluting of 10 times of 3mol/l of reuse, eluent is with 3 times ethyl acetate extraction 9 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 83.6%)
(3) get active component of red sage root 100 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 200 grams, pharmaceutic adjuvant sodium chloride 200 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 100 grams, pharmaceutic adjuvant sucrose 100 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.
Embodiment 3
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 7 times carries out supersound extraction, time is 25 minutes, frequency of oscillation is 30KHz, and the control temperature is a room temperature, extracts 3 times, merge extractive liquid, filters, it is 1.08 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 55%, leaves standstill 24 hours in 2 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 9.5, boiled 1 hour, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2.5 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 4 times of washings, eluent discards, the sodium chloride eluting of 7 times of 1.5mol/l of reuse, eluent is with 1 times ethyl acetate extraction 4 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 82.9%)
(3) get active component of red sage root 85 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 58, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 170 grams, pharmaceutic adjuvant glucose 230 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 85 grams, pharmaceutic adjuvant lactose 115 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.
Embodiment 4
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 8 times carries out supersound extraction, time is 30 minutes, frequency of oscillation is 40KHz, and the control temperature is a room temperature, extracts 3 times, merge extractive liquid, filters, it is 1.10 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 60%, leaves standstill 24 hours in 0 ℃-10 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 10, boiled 1.5 hours, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2.5 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 5 times of washings, eluent discards, the sodium chloride eluting of 8 times of 2mol/l of reuse, eluent is with 2 times ethyl acetate extraction 5 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 83.4%)
(3) get active component of red sage root 90 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 180 grams, pharmaceutic adjuvant sodium chloride 220 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 90 grams, pharmaceutic adjuvant mannitol, sucrose 110 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.。
Embodiment 5
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 9 times carries out supersound extraction, time is 35 minutes, frequency of oscillation is 60KHz, and the control temperature is a room temperature, extracts 3 times, merge extractive liquid, filters, it is 1.12 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 65%, leaves standstill 24 hours in 6 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 10, boiled 2 hours, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2.5 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 5 times of washings, eluent discards, the sodium chloride eluting of 9 times of 3mol/l of reuse, eluent is with 2.5 times ethyl acetate extraction 8 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 82.8%)
(3) got Radix Salviae Miltiorrhizae and imitated position 95 grams, added the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 190 grams, pharmaceutic adjuvant 210 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 95 grams, pharmaceutic adjuvant mannitol, lactose 105 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.
Embodiment 6
(1) gets red rooted salvia 4000 grams, chopping is put in the supersound extraction jar, and the water that adds 8 times carries out supersound extraction, time is 45 minutes, frequency of oscillation is 85KHz, and the control temperature is a room temperature, extracts 3 times, merge extractive liquid, filters, it is 1.14 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 65%, leaves standstill 24 hours in 7 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 10, boiled 2 hours, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 3 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 4 times of washings, eluent discards, the sodium chloride eluting of 7 times of 2mol/l of reuse, eluent is with 2 times ethyl acetate extraction 7 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 83.5%)
(3) get active component of red sage root 98 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 of aqueous injection;
(4) get active component of red sage root 196 grams, pharmaceutic adjuvant sodium chloride 204 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 1000 bottles of infusion solutions;
(5) get active component of red sage root 98 grams, pharmaceutic adjuvant sucrose, lactose 102 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 1000 bottles of injectable powder.
Embodiment 7
(1) gets red rooted salvia 40000 grams, chopping is put in the supersound extraction jar, and the water that adds 8 times carries out supersound extraction, time is 40 minutes, frequency of oscillation is 60KHz, and the control temperature is a room temperature, extracts 3 times, merge extractive liquid, filters, it is 1.10 (50 ℃) that filtrate is concentrated into relative density for 60 ℃, adds ethanol and makes and contain alcohol amount and reach 60%, leaves standstill 24 hours in 4 ℃, filter, it is extractum cold preservation (4 ℃) 48 hours about 1.05 (50 ℃) that filtrate is concentrated into relatively density, filters, and filtrate adds alkali liquor, and to transfer pH value be 10, boiled 2 hours, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with polar macroporous adsorbent resin column on the above-mentioned solution, earlier with 4 times of washings, eluent discards, the sodium chloride eluting of 8 times of 2mol/l of reuse, eluent is with 2 times ethyl acetate extraction 6 times, merge ethyl acetate liquid, the reclaim under reduced pressure ethyl acetate is to most, and vacuum drying below 60 ℃ is able to danshensu effective site; (through check and analysis experiment, content of Danshensu 82.9%)
(3) get active component of red sage root 1000 grams, add the dissolving of injection water fully, transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 10000 of aqueous injection;
(4) get active component of red sage root 2000 grams, pharmaceutic adjuvant glucose 2000 grams add the dissolving of injection water fully, and transferring pH value with meglumine is 5-8, uses the 0.22um filtering with microporous membrane, and filtrate is carried out fill, and check obtains 10000 bottles of infusion solutions;
(5) get active component of red sage root 1000 grams, pharmaceutic adjuvant mannitol, sucrose, lactose 1000 grams add the dissolving of injection water fully, use the 0.22um filtering with microporous membrane, and filtrate fill drying obtains 10000 bottles of injectable powder.
Claims (2)
1. the preparation of drug combination method of a danshensu is characterized in that:
(1) gets red rooted salvia, chopping is put in the supersound extraction jar, adds 6-10 water doubly and carries out supersound extraction, time is 20-50 minute, and frequency of oscillation is 20-100KHz, and the control temperature is a room temperature, extract 2-4 time, merge extractive liquid, filters, and filtrate concentrates at 60 ℃, relative density is 1.05-1.15 during to 50 ℃, add ethanol and make the alcohol amount of containing reach 50%-70%, left standstill 24 hours in 0 ℃-10 ℃, filter, relative density was about 1.05 extractum when filtrate was concentrated into 50 ℃, cold preservation is 48 hours in the time of 4 ℃, filters, and it is 9-11 that filtrate adds the lye pH adjustment value, boiled 0.5-3 hour, put coldly, 4 ℃ of cold preservation 24 hours is filtered, it is 2-3 that filtrate is regulated pH value with hydrochloric acid, obtains solution;
(2) with macroporous adsorptive resins on the above-mentioned solution, doubly wash with 3-6 earlier, eluent discards, the sodium chloride eluting of reuse 6-10 times 1-3mol/L, eluent merges ethyl acetate liquid with 0.5-3 ethyl acetate extraction doubly 3-9 time, and the reclaim under reduced pressure ethyl acetate is to using up, vacuum drying below 60 ℃ obtains danshensu effective site, and wherein the content of active component of red sage root danshensu is not less than 80%;
(3) formulation preparation:
The aqueous injection preparation: getting active component of red sage root 80-100 weight portion, add the dissolving of injection water fully, is 5-8 with the meglumine adjust pH, and with 0.22 μ m filtering with microporous membrane, filtrate is carried out fill, and check obtains aqueous injection;
The infusion solution preparation: get active component of red sage root 160-200 weight portion, pharmaceutic adjuvant 200-240 weight portion adds the dissolving of injection water fully, is 5-8 with the meglumine adjust pH, and with 0.22 μ m filtering with microporous membrane, filtrate is carried out fill, and check obtains infusion solution;
The injectable powder preparation: get active component of red sage root 80-100 weight portion, pharmaceutic adjuvant 100-120 weight portion adds the dissolving of injection water fully, and with 0.22 μ m filtering with microporous membrane, filtrate fill drying obtains injectable powder.
2. according to the preparation of drug combination method of the described a kind of danshensu of claim 1, it is characterized in that the macroporous adsorbent resin that uses is polar macroporous adsorption resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410074650 CN1264506C (en) | 2004-09-10 | 2004-09-10 | Pharmaceutical combination containing red sage root element and preparation method thereof |
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| CN 200410074650 CN1264506C (en) | 2004-09-10 | 2004-09-10 | Pharmaceutical combination containing red sage root element and preparation method thereof |
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| CN1264506C true CN1264506C (en) | 2006-07-19 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1919247B (en) * | 2005-08-24 | 2011-02-16 | 天津天士力制药股份有限公司 | Chinese medicine for treating cardiovascular and cerebrovascular disease |
| CN101342236B (en) * | 2007-07-11 | 2011-07-20 | 天津药物研究院 | Method for preparing red sage root extract, red sage root extract and medicament composition containing the same |
| CN101985417B (en) * | 2010-11-02 | 2013-06-05 | 贵州景峰注射剂有限公司 | Process for extracting salvianic acid A |
| CN104672082B (en) * | 2015-02-10 | 2016-08-17 | 天津大学 | A kind of use the method for danshensu in macroporous adsorbing resin for purification fermentation liquid |
| CN104971091B (en) * | 2015-06-03 | 2019-08-13 | 兴明生物医药技术(上海)有限公司 | It is a kind of to be used to prepare the preparation method and applications for promoting the extract of Myocardial Regeneration drug |
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