CN1256154C - Germ interstitial antisenility technology - Google Patents
Germ interstitial antisenility technology Download PDFInfo
- Publication number
- CN1256154C CN1256154C CN 03143255 CN03143255A CN1256154C CN 1256154 C CN1256154 C CN 1256154C CN 03143255 CN03143255 CN 03143255 CN 03143255 A CN03143255 A CN 03143255A CN 1256154 C CN1256154 C CN 1256154C
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- CN
- China
- Prior art keywords
- germ
- interstitial
- reproduction
- testes
- interstitial cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005516 engineering process Methods 0.000 title description 2
- 230000003005 anti-senility effect Effects 0.000 title 1
- 210000002570 interstitial cell Anatomy 0.000 claims abstract description 27
- 210000004027 cell Anatomy 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
- 210000001550 testis Anatomy 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 210000002966 serum Anatomy 0.000 claims abstract description 4
- 208000028867 ischemia Diseases 0.000 claims abstract description 3
- 210000001519 tissue Anatomy 0.000 claims abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000012531 culture fluid Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 2
- 210000002808 connective tissue Anatomy 0.000 claims description 2
- -1 dimethyl sulfoxine Chemical compound 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 238000002054 transplantation Methods 0.000 abstract description 3
- 210000003205 muscle Anatomy 0.000 abstract description 2
- 230000003712 anti-aging effect Effects 0.000 abstract 2
- 102000008186 Collagen Human genes 0.000 abstract 1
- 108010035532 Collagen Proteins 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 241000282887 Suidae Species 0.000 abstract 1
- 229920001436 collagen Polymers 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 102000038379 digestive enzymes Human genes 0.000 abstract 1
- 108091007734 digestive enzymes Proteins 0.000 abstract 1
- 210000004602 germ cell Anatomy 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 239000000647 testicular hormone Substances 0.000 abstract 1
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 230000032683 aging Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000008092 positive effect Effects 0.000 description 2
- 230000036299 sexual function Effects 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 229960003604 testosterone Drugs 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 235000016299 Canarium odontophyllum Nutrition 0.000 description 1
- 244000001582 Canarium odontophyllum Species 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 206010033307 Overweight Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to an anti aging technique for germ interstitial cells, which is characterized in that germ interstitial cells of animal testes are transplanted. The technique specifically comprises: firstly, testes of young pigs are taken and are put into an ice kettle, and after two hours of cold ischemia, the testes are sent to a laboratory; secondly, germ interstitial cells are cultured; tissues in the testes are sheared into tablets and are ground, collagen digestive enzymes are added for further purification and sieving, and then, 1640 nutrient liquid containing AB blood serum is added; the testes are put into a CO2 incubator to be cultured, and well growing cells are collected and reserved. The application method comprises: after 2*10<7> germ interstitial cells are collected, the germ interstitial cells are injected into muscles of upper legs of human bodies, and then, the transplantation is completed. The technique of the present invention has the advantages that the anti-aging problem of human, which people always eager to solve, is preliminary solved, and the transplantation of germ interstitial cells not only can enhance the level of testicular hormones and promote the generation of germ cells, but also can supplement effective components which are not discovered yet. The successful development of germ interstitial cells obtains an obvious effect on clinical application.
Description
The present invention relates to a kind of cultural method of reproduction Interstitial cell.
Background technology
Open old and feeble secret, it is the wonderful dream that people dream of always, prove after deliberation, the mankind aging is relevant with reproduction Interstitial cell radix, that is to say, the minimizing in vivo of reproduction Interstitial cell radix causes the organism metabolism gesture to weaken, and makes the organism metabolism decay, thereby causes the aging and the lost of life.This conclusion and China's traditional medicine this theory of kidney that the kidney being the origin of congenital constitution matches.According to the principle the kidney invigorating of Chinese medicine " dirty " with dirty benefit, through the cooking or eat stomach raw after active substance all destroyed, the reproduction Interstitial cell loses activity, so do not reach the purpose of real the kidney invigorating.
Summary of the invention
Technical problem to be solved by this invention provides a kind of cultural method of reproduction Interstitial cell.
The technical solution adopted for the present invention to solve the technical problems:
Concrete grammar of the present invention is as follows:
(1) the reproduction Interstitial cell is cultivated: the young pig testis that will choose is in cold ischemia 2 hours, cut off and remove sheath, grinding then is cut into small pieces testis inner tissue, remove connective tissue, adding collagenolysis enzyme is further purified and sieves, and subsequently, adds the RPMI-1640 that contains AB serum, the culture fluid that adds 10ml in each culture bottle, every milliliter contains number of cells is 10
3-10
5Individual, culture bottle is put into CO
2Cultivate in the incubator, temperature is 37 ℃ of constant temperature, and uses Hank, the flushing of s liquid, and incubation time is 6-7 days, when the cell well-grown, no bacterial growth is then collected standby.
(2) the reproduction Interstitial cell is preserved: be divided in test tube and sealing after putting into the dimethyl sulfoxine of 1.4mol/L and PH and be 7.2 Hank ' s liquid 2ml in culture fluid, temperature is 0 ℃-4 ℃, 4 ℃ left standstill 30-40 minute, be cooled to 0 ℃ with 6-8 minute speed, after being cooled to-80 ℃ by 0.4 ℃ of-0.6 ℃/min speed again, put into-196 ℃ of nitrogen liquid again; During use, with 40 ℃ of warm water quick rotation rewarmings, put in the CO2 incubator of back 37 ℃ then after constant temperature culture 48-72 hour, cell brings back to life good, and no bacterial growth just can use.
During use, after the reproduction Interstitial cell was collected, its quantity reached 2 * 10
7The time, be injected in the human body femoribus internus muscle, promptly accuse and finish transplanting.
It is that DABAI and platform are nascent 2-3 month boar of Du Ke pig hybridization that described young pig is selected English for use.
The invention has the beneficial effects as follows the cultural method that a kind of reproduction Interstitial cell is provided, the reproduction Interstitial cell is to produce male sharp main cell, not only can improve the testosterone level by transplanting, promote sexual cell to generate, but also augmented still undiscovered active ingredient.The successful research and development of reproduction Interstitial cell have obtained positive effect in clinical practice, tentatively solved people and thirsted for the human defying age problem that solves always.
The specific embodiment
Embodiment: carry out concrete operations by the technical scheme in the foregoing invention content part and get final product.
Utilize the present invention that 30 routine volunteers are carried out clinical trial, tired comprehensive, hypofunction is arranged among the 30 routine volunteers, nervously breathe hard, obesity, climacteric syndrome, arteriosclerosis.It is 9.8 * 10 that the reproduction Interstitial cell is transplanted minimum number
6Individual, mostly be 3.6 * 10 most
7Individual.The reproduction Interstitial cell is transplanted the back and was begun onset in 3-8 days, and 24 examples have positive effect, and fatigue syndrome obviously improves, and sexual function obviously improves, to juvenile overweight people, and not only fat-reducing, and also health also obviously grows tall.Effective percentage 80%, no change 6 examples account for 20%.Observation all more than half a year, was not found side reaction after this group volunteer transplanted.
Testosterone situation of change in the serum before and after transplanting: average out to 633ng/dl before transplanting, transplant back average out to 767ng/dl, on average increase 15.6nf/dl, the highest value added reaches 22.5%.Transplantation effect has obvious relation with transplanting reproduction Interstitial cell quantity, and 6 routine no change persons transplant quantity 9.8 * 10
6Individual.
Its transplanting reproduction Interstitial cell quantity of the first of this clinical trial is 3.6 * 10
7Individual, begin generating effect after five days, in high spirits, the tired disappearance, sexual function obviously increases, and beyond thought effect occurred, tentatively reaches the old purpose of kidney-nourishing and anti-senile.
Claims (1)
1, a kind of cultural method of reproduction Interstitial cell is characterized in that:
(1) the reproduction Interstitial cell is cultivated: the young pig testis that will take out is cut off in 2 hours and is removed sheath at cold ischemia, grinding then is cut into small pieces testis inner tissue, remove connective tissue, adding the collagenolysis enzyme is further purified, sieves, subsequently, add the RPMI-1640 that contains AB serum, add the culture fluid of 10ml in each culture bottle, every milliliter contains number of cells is 10
3-10
5Individual, culture bottle is put into CO
2Cultivate in the incubator, temperature is 37 ℃ of constant temperature, and uses Hank ' s liquid regularly to wash, and incubation time is 6-7 days, and when the cell well-grown, no bacterial growth is then collected standby;
(2) the reproduction Interstitial cell is preserved: the dimethyl sulfoxine and the PH that add 1.4mol/L in the culture fluid with reproduction Interstitial cell
7.2Hank ' s liquid 2ml after, in the test tube of packing into and the sealing, temperature is 0 ℃-4 ℃, 4 ℃ left standstill 30-40 minute, were cooled to 0 ℃ with 6-8 minute speed, be cooled to-80 ℃ by 0.4-0.6 ℃/min speed again after, put into-196 ℃ of nitrogen liquid again; During use,, put into CO then with 40 ℃ of warm water quick rotation rewarmings
2In the incubator 37 ℃ after constant temperature culture 48-72 hour, cell brings back to life good, and no bacterial growth just can use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03143255 CN1256154C (en) | 2003-08-20 | 2003-08-20 | Germ interstitial antisenility technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03143255 CN1256154C (en) | 2003-08-20 | 2003-08-20 | Germ interstitial antisenility technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1513564A CN1513564A (en) | 2004-07-21 |
| CN1256154C true CN1256154C (en) | 2006-05-17 |
Family
ID=34240382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03143255 Expired - Fee Related CN1256154C (en) | 2003-08-20 | 2003-08-20 | Germ interstitial antisenility technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1256154C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224312B (en) * | 2007-01-19 | 2011-02-16 | 中国人民解放军第二军医大学 | Tissue-engineered androgen secretory tissue implant |
-
2003
- 2003-08-20 CN CN 03143255 patent/CN1256154C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1513564A (en) | 2004-07-21 |
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