CN1253552C - Streptomyces griseoalbus - Google Patents
Streptomyces griseoalbus Download PDFInfo
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- CN1253552C CN1253552C CN 03124240 CN03124240A CN1253552C CN 1253552 C CN1253552 C CN 1253552C CN 03124240 CN03124240 CN 03124240 CN 03124240 A CN03124240 A CN 03124240A CN 1253552 C CN1253552 C CN 1253552C
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- streptomyces
- griseoalbus
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Abstract
The present invention relates to the microbial field, particularly to a microbe with an insecticidal action. The microbe is characterized in that the microbe is named as Streptomyces griseoalbusYIM30823 which is preserved in a preservation organization designated by the State Intellectual Property Bureau, the preservation date is Feb. 14th, 2003, the name of the preservation organization is the China Center for Type Culture Collection, and the preservation number is CCTCC NO. M203014. The active bacterial strain of the present invention has insecticidal activity, the fermentation liquor of the microbe has an action of killing injurious insects of Aphis medicaginis, Tetranychuscinnabarius, Leneania separata, etc., and the present invention has stable and durable effect, and large potentiality for developing novel, effective and non-poisonous natural insecticide.
Description
Technical field
The present invention relates to microbial technology field, particularly relate to a kind of microorganism with insecticidal action.
Background technology
Aphid (Aphis medicaginis), acarid (Tetranychus cinnabarius), armyworm insects such as (Leneaniaseparata) are the primary pests of farm crop.Therefore, it is very important suppressing above-mentioned insect.
Summary of the invention
The purpose of this invention is to provide microorganism-Streptomyces griseoalbus that a kind of its fermented liquid has insecticidal action.
Its fermented liquid is also to Candida albicans, and subtilis has anti-microbial activity.
Streptomyces griseoalbus called after Streptomyces griseoalbus Streptomyces griseoalbus YIM30823 of the present invention, deposit number is CCTCC NO:M203014.
Separate in the pedotheque of Streptomyces griseoalbus system of the present invention pasture collection from Yunnan Province of China and obtain.Now be deposited in the preservation of specified depositary institution of State Intellectual Property Office, depositary institution's title: Chinese typical culture collection center, preservation date are on February 14th, 2003, and deposit number is CCTCC NO:M203014.
Streptomycete of the present invention is well-grown on most of substratum, and aerial hyphae is greyish white, white, and substrate mycelium is orange-yellow to tawny.The spore chain spirrillum.The spore rod-short, spore surface has fold.Gelatine liquefication, milk solidify, peptonize and be positive, and starch hydrolysis, nitrate reductase are negative, and do not produce hydrogen sulfide, form melanochrome.Can utilize glucose, semi-lactosi, fructose wood sugar, raffinose, rhamnosyl, melibiose, sweet and pure, glycerine, N.F,USP MANNITOL, sorbyl alcohol, sodium-acetate.Cell walls contains L-diaminopimelic acid and glycine, full cell hydrolyzate atypism sugar.
Culture condition:
(1) slant culture condition: substratum: glucose 4g; Yeast extract paste 4g; Malt extract 5g; Vitamin complex 3.75mg/L; Agar 15g; Distilled water water 1000ml, pH7.2; Culture temperature is 28 ℃, and incubation time was 1 week.
(2) seed culture condition: insert the seed liquor shake-flask culture 36 hours from inclined-plane picking part mycelium.Seed culture medium: dextrin 120g; Soyflour 40g; Yeast extract paste 2g; Tryptophane 0.5g; Beta-alanine 5g; Sal epsom 0.5g; Ammonium phosphate 0.2g; Distilled water 1000ml, pH7.2, culture temperature is 28 ℃.
(3) fermentation culture conditions: receive in the seed culture medium shake-flask culture 6 days by 10% inoculum size.Substratum: soyflour 10g; Glucose 10g; Peptone 3g; Sodium-chlor 2.5g; Lime carbonate 2g; Distilled water 1000ml, pH7.2, culture temperature is 28 ℃.Obtain fermented liquid.
This fermented liquid insecticidal activity screening process figure:
The ethanol extracting 4hr of 20ml fermented liquid → 1: 1 → centrifugal → concentrate → water dilutes 20,200,2000 times respectively.
Select different insect live bodies for use, directly spray the back in 12,24,36,48hr observes death condition.
This fermented liquid insecticidal activity The selection result:
The fermented liquid of bacterial strain YIM30823 is 100% to the killing rate of aphid (Aphis medicaginis), acarid (Tetranychuscinnabarius), armyworm three kinds of insects such as (Leneania separata).
The fermented liquid of this bacterial strain has the effect of killing aphid (Aphis medicaginis), acarid (Tetranychuscinnabarius), armyworm insects such as (Leneania separata), has the great potential of therefrom developing novel, efficient, nontoxic natural drug.
Streptomycete called after Streptomyces griseoalbus of the present invention, on February 14th, 2003 in China's typical culture collection center preservation, depositary institution is called for short: CCTCC, deposit number is M203014, the depositary institution address: Wuhan, Luo Jiashan, in the Wuhan University, postcode 430072.
Description of drawings
Fig. 1 is the electromicroscopic photograph of Streptomyces griseoalbus of the present invention on yeast extract paste-malt extract substratum.
Fig. 2 is the cultural characteristic of Streptomyces griseoalbus of the present invention.
Fig. 3 is the physiological and biochemical property of Streptomyces griseoalbus of the present invention.
Fig. 4 utilizes situation for the carbon nitrogen source of Streptomyces griseoalbus of the present invention.
Fig. 5 is that Streptomyces griseoalbus of the present invention and part correlation bacterium are in the 16SrDNA of GenBank database sequence and accession number.
Fig. 6 is Streptomyces griseoalbus of the present invention and the part correlation bacterial strain phylogenetic tree according to 16S rDNA sequence construct.
Embodiment
Embodiment:
The pedotheque that gather in the pasture from Yunnan Province of China is cultivated by following condition in the laboratory, can obtain Streptomyces griseoalbus of the present invention.
(1) culture condition:
1. slant culture condition: substratum: glucose 4g; Yeast extract paste 4g; Malt extract 5g; Vitamin complex 3.75mg/L; Agar 15g; Distilled water water 1000ml, pH7.2; Culture temperature is 28 ℃, and incubation time was 1 week.
2. seed culture condition: insert the seed liquor shake-flask culture 36 hours from inclined-plane picking part mycelium.Seed culture medium: dextrin 120g; Soyflour 40g; Yeast extract paste 2g; Tryptophane 0.5g; Beta-alanine 5g; Sal epsom 0.5g; Ammonium phosphate 0.2g; Distilled water 1000ml, pH7.2, culture temperature is 28 ℃.
Cultivate by following fermentation culture conditions, can obtain fermented liquid: receive in the seed culture medium shake-flask culture 6 days by 10% inoculum size.Substratum: soyflour 10g; Glucose 10g; Peptone 3g; Sodium-chlor 2.5g; Lime carbonate 2g; Distilled water 1000ml, pH7.2, culture temperature is 28 ℃.
The 16S rDNA partial sequence of streptomycete of the present invention is analyzed as follows:
GAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAACCGGCTTCGGCTGGGGATTAGTGGCGAACGGGTGAGT
AACACGTGGGCAATCTGCCCTGCACTCTGGGAC is from GCCCTGGAAACGGGGTCTAATACCGGATATTACCTGGTGAGGCAT
CTTACTGGGTGGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGGGGTGATGGCCTACCAAGG
CGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCA
GTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTC
TTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATAC
GTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGG
GCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAA
TGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGG
AGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTTGTCCGT
GCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGC
ACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAACATCCAG
AGATGGGTGCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC
CCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGGTC
AACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTA
CAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCC
ATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTC
ACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGAGTCGTCGAAGGTGGGACTGGCGAT
TGGGACGAAGTCGTAACAAGGTAGCCGTACGGAAGGA
Claims (2)
1. Streptomyces griseoalbus, it is characterized in that called after Streptomyces griseoalbus Streptomycesgriseoalbus YIM 30823, specified depositary institution of State Intellectual Property Office preservation now, preservation date is on February 14th, 2003, depositary institution's title: Chinese typical culture collection center, deposit number CCTCCNO:M203014.
2. Streptomyces griseoalbus according to claim 1 is characterized in that the 16SrDNA partial sequence of this bacterial classification is as follows:
GAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAACCGGCTTCGGCTGGGGATTAGTGGC
GAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAAT
ACCGGATATTACCTGGTGAGGCATCTTACTGGGTGGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGC
CTATCAGCTTGTTGGTGGGGTGATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACC
GGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG
GGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCA
GGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTA
ATACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCG
GATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGGA
GATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGAT
CTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGT
CCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTTGTCCGTGCCGCAGCTAACGCAT
TAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAA
GCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAA
ACATCCAGAGATGGGTGCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTC
GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGG
GTGATGGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCA
TGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGT
GGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAG
TCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTC
ACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGAGTCGTCGAAGGT
GGGACTGGCGATTGGGACGAAGTCGTAACAAGGTAGCCGTACGGAAGGA。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03124240 CN1253552C (en) | 2003-04-29 | 2003-04-29 | Streptomyces griseoalbus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03124240 CN1253552C (en) | 2003-04-29 | 2003-04-29 | Streptomyces griseoalbus |
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|---|---|
| CN1542130A CN1542130A (en) | 2004-11-03 |
| CN1253552C true CN1253552C (en) | 2006-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03124240 Expired - Fee Related CN1253552C (en) | 2003-04-29 | 2003-04-29 | Streptomyces griseoalbus |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967629B (en) * | 2016-11-21 | 2021-11-16 | 云南大学 | Soil actinomycete and application thereof in preventing and controlling aphids |
| CN111593002B (en) * | 2018-12-24 | 2021-11-09 | 吉林省林业科学研究院 | Preparation method of biocontrol preparation |
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