CN1251689C - Veal-blood protein-removed extract infusion and its preparation process - Google Patents
Veal-blood protein-removed extract infusion and its preparation process Download PDFInfo
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- CN1251689C CN1251689C CNB2004100136436A CN200410013643A CN1251689C CN 1251689 C CN1251689 C CN 1251689C CN B2004100136436 A CNB2004100136436 A CN B2004100136436A CN 200410013643 A CN200410013643 A CN 200410013643A CN 1251689 C CN1251689 C CN 1251689C
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- calf blood
- osmotic pressure
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- 239000008280 blood Substances 0.000 title claims abstract description 69
- 238000002360 preparation method Methods 0.000 title claims description 26
- 238000001802 infusion Methods 0.000 title description 8
- 244000309466 calf Species 0.000 claims abstract description 75
- 210000004369 blood Anatomy 0.000 claims abstract description 68
- 230000003204 osmotic effect Effects 0.000 claims abstract description 49
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 30
- 150000001413 amino acids Chemical class 0.000 claims abstract description 18
- 238000005516 engineering process Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 15
- 210000002966 serum Anatomy 0.000 claims abstract description 6
- 239000007787 solid Substances 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims description 60
- 239000000243 solution Substances 0.000 claims description 60
- 238000003756 stirring Methods 0.000 claims description 21
- 235000001014 amino acid Nutrition 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 239000006228 supernatant Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000000047 product Substances 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 12
- 239000008215 water for injection Substances 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 6
- 238000005262 decarbonization Methods 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 229930195725 Mannitol Natural products 0.000 claims description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 3
- 239000000594 mannitol Substances 0.000 claims description 3
- 235000010355 mannitol Nutrition 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 210000003462 vein Anatomy 0.000 abstract description 13
- 239000003814 drug Substances 0.000 abstract description 10
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 10
- 210000001367 artery Anatomy 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
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- 230000002490 cerebral effect Effects 0.000 abstract description 5
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- 201000006474 Brain Ischemia Diseases 0.000 abstract description 2
- 206010008120 Cerebral ischaemia Diseases 0.000 abstract description 2
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- 206010008118 cerebral infarction Diseases 0.000 abstract description 2
- 230000006870 function Effects 0.000 abstract description 2
- 230000008733 trauma Effects 0.000 abstract description 2
- 239000003918 blood extract Substances 0.000 abstract 8
- 206010020751 Hypersensitivity Diseases 0.000 abstract 1
- 208000030961 allergic reaction Diseases 0.000 abstract 1
- 230000019522 cellular metabolic process Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002093 peripheral effect Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 208000019553 vascular disease Diseases 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 13
- 210000004556 brain Anatomy 0.000 description 10
- 102000004506 Blood Proteins Human genes 0.000 description 7
- 108010017384 Blood Proteins Proteins 0.000 description 7
- 230000003925 brain function Effects 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000001990 intravenous administration Methods 0.000 description 4
- 230000007654 ischemic lesion Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 206010002198 Anaphylactic reaction Diseases 0.000 description 2
- 230000036783 anaphylactic response Effects 0.000 description 2
- 208000003455 anaphylaxis Diseases 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 208000005230 Leg Ulcer Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
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- 230000006567 cellular energy metabolism Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
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- 230000003628 erosive effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- -1 nitrogen-containing compound Chemical class 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
- 231100000075 skin burn Toxicity 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
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- 231100000397 ulcer Toxicity 0.000 description 1
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to deproteinized calf blood extract solution and technology for preparing the solution. Amino acid and small peptide in deproteinized calf blood extract solution can be polymerized into peptide with large molecular weight and peptide with high molecular peptide, and thus, medicine generates antigenicity, the biological activity of the medicine is reduced, and clinical allergic reaction is increased. The deproteinized calf blood extract solution comprises deproteinized calf blood extract and osmotic pressure regulating agents, wherein the deproteinized calf blood extract is extracted from fresh calf blood or blood serum, solid substances of the deproteinized calf blood extract contain 20.0 to 30.0 parts of total nitrogen and 12.5 to 18.75 parts of free amino acid by weight and have the pH value of 5.5 to 8.0 and the molecular weight of 50 to 100, and the deproteinized calf blood extract is yellowish or flaxen transparent liquid. The deproteinized calf blood extract solution is used for treating cerebral cell metabolism dysbolism diseases such as cerebral ischemia, cerebral dementia, cerebral trauma, insufficiency of cerebral functions, etc., and can also be used for treating circulatory disturbance peripheral arteries and veins, and artery vascular diseases caused by the circulatory disturbance.
Description
Technical field:
The present invention relates to a kind of Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method.
Background technology:
Its main component of calf blood protein-removed extraction solution is an aminoacid, little peptide and other nitrogen-containing compound, aminoacid and little peptide under naturalness mutually polymerization form the peptide of macromolecule, and peptide can be grouped to the macromolecule peptide, separate out or exist in solution with polymer form with macromolecule peptide form, make medicine produce antigenicity and reduced the biological activity of medicine, Here it is, and calf blood protein-removed extraction solution causes the main cause that clinical anaphylaxis increases, prolongation along with the holding time, adverse reaction rate also increases immediately, and be that calf blood protein-removed extraction solution is dissolved in sugar when clinical application, use in the saline, so not only increased the contaminated probability of medicine, influence the safety that medicine uses, but also increased medical personnel's workload.
Summary of the invention:
It is simple to the purpose of this invention is to provide a kind of preparation technology, easy to use, easily the Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method that stores.
The object of the present invention is achieved like this:
The preparation technology of calf blood protein-removed extraction transfusion, get calf blood protein-removed extraction solution and osmotic pressure regulator and preparation by weight, get parts by weight and be 16500～24800 calf blood, filter, learn from else's experience calf blood 16000～24000 after filtering, adjust pH is to alkalescence, centrifugal, parts by weight are 15000～23000 supernatant, adjust pH to 7.5～8, centrifugal, get parts by weight and be 12000～20000 supernatant, adjust pH to 6.5～7.5, in 70 ℃～90 ℃ heat treatments, centrifugal, 60 ℃～80 ℃ decompressed concentrates, it is 3000～5000 concentrated solution that concentrating under reduced pressure gets parts by weight, ultrafiltration, molecular cut off is 5000～10000 dalton relatively, make parts by weight and be 2500～4500 calf blood protein-removed extraction solution, get osmotic pressure regulator 50000～100000 by weight, add 20～40 times of water dissolutioies and get osmotic pressure regulator solution, add 0.01～5% active carbon again, heating, filter dense osmotic pressure regulator solution, again above-mentioned described dense osmotic pressure regulator solution is mixed with calf blood protein-removed extraction solution, add the water for injection of 70～140 times of amounts, stir evenly, make the calf blood protein-removed extraction transfusion, goods are little yellow or faint yellow clear liquid.
The preparation technology of above-mentioned calf blood protein-removed extraction transfusion, the preparation technology of described dense osmotic pressure regulator solution is: get parts by weight and be 50000～100000 osmotic pressure regulator, the water for injection that adds 20～40 times of amounts, stirring and dissolving is made into osmotic pressure regulator solution, adds 0.01～5% active carbon again in described solution, stir evenly, be heated to boiling, filtering decarbonization, fine straining get dense osmotic pressure regulator solution.
The calf blood protein-removed extraction transfusion of above-mentioned preparation technology's preparation, its composition comprises: calf blood protein-removed extraction and osmotic pressure regulator, described calf blood protein-removed extraction is to extract from calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, aminoacid, its parts by weight are aminoacid 20.0～30.0, total nitrogen 12.5～18.75, osmotic pressure regulator 50～100, pH value should be 5.5～8.0, and this product is little yellow or faint yellow clear liquid.
Above-mentioned calf blood protein-removed extraction transfusion, described osmotic pressure regulator is glucose or sodium chloride or mannitol.
The invention has the advantages that:
1. calf blood protein-removed extraction transfusion of the present invention is to add dense osmotic pressure regulator solution in calf blood protein-removed extraction solution, through being mixed and made into, it has overcome calf blood protein-removed extraction solution and has easily caused clinical anaphylactoid defective, its drug level is compared than entry needle and is descended 10 times, so in storing process, reduced the generation that medicine is separated out, thereby reduced harm to patient, and the calf blood protein-removed extraction transfusion need not be diluted in use, not only made things convenient for but also reduced the pollution that may cause in the preparation, so clinical application is comparatively safe, and more more options are provided to medical personnel.
2. calf blood protein-removed extraction transfusion contains inorganic elementss such as multiple small-molecule active substance and calcium, phosphorus, ferrum, its pharmacological action is by promoting picked-up and the utilization of cell to glucose, promote picked-up and the utilization of cell to oxygen, thereby ATP is generated to be increased, the cell energy amount is improved, when (low blood oxygen, substrate deficiency) and energy requirement increase under function defective, the downtrod situation of metabolism, can promote the cellular energy metabolism, improve cell function, its development and application prospect are very wide.
3. the present invention compares with calf blood protein-removed extraction solution, and this product has reduced the generation that medicine is separated out in the process of storage, and good stability has reduced the anaphylaxis incidence rate, has guaranteed patient's drug safety.
4. the preparation technology of this product is simple, advanced, easily operation.
5. this product can promote picked-up and the utilization of cell to glucose and oxygen, under low blood oxygen and energy such as need increase at situation, this product can promote energy metabolism, increase the blood flow of internal organs such as the heart, brain, liver, microcirculation improvement, be applicable to the not congruent brain cell dysbolismus of cerebral ischemia, brain dementia, cerebral trauma and brain function disease, clinically also can be used for treating tip tremulous pulse, venous circulation obstacle and the artery vessel disease that causes, leg ulcer, be used for skin grafting, in treatment skin burn, scald, erosion; Also obtained extraordinary effect in healing of wound (wound, decubital ulcer), radio-induced skin, the mucosa injury process.
Description of drawings: accompanying drawing 1 is technology path of the present invention and process chart.
Below in conjunction with description of drawings the specific embodiment of the present invention:
Embodiment 1:
Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method, its composition comprises: calf blood protein-removed extraction and osmotic pressure regulator, described calf blood protein-removed extraction is to extract from calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, aminoacid, its parts by weight are aminoacid 20.0, total nitrogen 12.5, osmotic pressure regulator 50, pH value should be 5.5, and this product is little yellow or faint yellow clear liquid.Its preparation technology is: get parts by weight and be 16500 calf blood, filter, parts by weight are 16000 coarse filtration liquid, adjust pH to 4.5, centrifugal, parts by weight are 15000 supernatant, adjust pH to 7.5, centrifugal, parts by weight are 12000 supernatant, get supernatant, adjust pH to 6.5, in 70 ℃ of heat treatments, centrifugal, it is 3000 concentrated solution that 60 ℃ of concentrating under reduced pressure get parts by weight, ultrafiltration, relatively molecular cut off is 5000 dalton, makes parts by weight and be 2500 calf blood protein-removed extraction solution for standby; Get parts by weight and be 50000 osmotic pressure regulator, add the water for injection of 20 times of amounts, stirring and dissolving, be made into osmotic pressure regulator solution, in described solution, add 0.01% active carbon again, stir evenly, be heated to boiling, filtering decarbonization, fine straining get dense osmotic pressure regulator solution for standby; Above-mentioned described dense osmotic pressure regulator solution is mixed with calf blood protein-removed extraction solution again, add the water for injection of 70 times of amounts, stir evenly, make the calf blood protein-removed extraction transfusion, goods are little yellow or faint yellow clear liquid.Its packing specification is 250ml: contain 30mg aminoacid; 18.75mg total nitrogen; 2.25g osmotic pressure regulator.This product is used for intravenous drip, and drip velocity is 2ml/min, to the brain ischemic lesions: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; Brain function is not reached brain dementia entirely: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; To artery vessel disease: 1 time on the one, a 250ml, vein slowly instils, and is a course of treatment all around.
Embodiment 2:
Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method, its composition comprises: calf blood protein-removed extraction and osmotic pressure regulator, described calf blood protein-removed extraction is to extract from calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, aminoacid, its parts by weight are aminoacid 30.0, total nitrogen 18.75, osmotic pressure regulator 100, pH value should be 8.0, and this product is little yellow or faint yellow clear liquid.Its preparation technology is: get parts by weight and be 24800 calf blood, filter, parts by weight are 24000 coarse filtration liquid, adjust pH to 5.0, centrifugal, parts by weight are 23000 supernatant, adjust pH to 8.0, centrifugal, parts by weight are 20000 supernatant, get supernatant, adjust pH to 7.5, in 90 ℃ of heat treatments, centrifugal, it is 5000 concentrated solution that 80 ℃ of concentrating under reduced pressure get parts by weight, ultrafiltration, relatively molecular cut off is 10000 dalton, makes parts by weight and be 4500 calf blood protein-removed extraction solution for standby; Get parts by weight and be 100000 osmotic pressure regulator, add the water for injection of 40 times of amounts, stirring and dissolving, be made into osmotic pressure regulator solution, in described solution, add 5% active carbon again, stir evenly, be heated to boiling, filtering decarbonization, fine straining get dense osmotic pressure regulator solution for standby; Above-mentioned described dense osmotic pressure regulator solution is mixed with calf blood protein-removed extraction solution again, add the water for injection of 140 times of amounts, stir evenly, make the calf blood protein-removed extraction transfusion, goods are little yellow or faint yellow clear liquid.Its packing specification is 250ml: contain 30mg aminoacid; 18.75mg total nitrogen; 2.25g osmotic pressure regulator.This product is used for intravenous drip, and drip velocity is 2ml/min, to the brain ischemic lesions: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; Brain function is not reached brain dementia entirely: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; To artery vessel disease: 1 time on the one, a 250ml, vein slowly instils, and is a course of treatment all around.
Embodiment 3:
Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method, its composition comprises: calf blood protein-removed extraction and osmotic pressure regulator, described calf blood protein-removed extraction is to extract from calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, aminoacid, its parts by weight are aminoacid 25.0, total nitrogen 15.5, osmotic pressure regulator 70, pH value should be 6.5, and this product is little yellow or faint yellow clear liquid.Its preparation technology is: get parts by weight and be 22800 calf blood, filter, parts by weight are 22000 coarse filtration liquid, adjust pH to 4.7, centrifugal, parts by weight are 22000 supernatant, adjust pH to 7.8, centrifugal, parts by weight are 15000 supernatant, get supernatant, adjust pH to 7.0, in 80 ℃ of heat treatments, centrifugal, it is 4000 concentrated solution that 70 ℃ of concentrating under reduced pressure get parts by weight, ultrafiltration, relatively molecular cut off is 8000 dalton, makes parts by weight and be 3500 calf blood protein-removed extraction solution for standby; Get parts by weight and be 80000 osmotic pressure regulator, add the water for injection of 30 times of amounts, stirring and dissolving, be made into osmotic pressure regulator solution, in described solution, add 3% active carbon again, stir evenly, be heated to boiling, filtering decarbonization, fine straining get dense osmotic pressure regulator solution for standby; Above-mentioned described dense osmotic pressure regulator solution is mixed with calf blood protein-removed extraction solution again, add the water for injection of 100 times of amounts, stir evenly, make the calf blood protein-removed extraction transfusion, goods are little yellow or faint yellow clear liquid.Its packing specification is 250ml: contain 30mg aminoacid; 18.75mg total nitrogen; 2.25g osmotic pressure regulator.This product is used for intravenous drip, and drip velocity is 2ml/min, to the brain ischemic lesions: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; Brain function is not reached brain dementia entirely: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; To artery vessel disease: 1 time on the one, a 250ml, vein slowly instils, and is a course of treatment all around.
Embodiment 4:
Small Ox Blood Protein removing Extraction Infusion And Its Preparation Method, remove fiber calf blood 496kg, filter, get coarse filtration liquid 481L, stir adding 3M HCl solution 3.6L down, adjust pH to 4.85, centrifugal, get supernatant 421L, stir adding 3M NaOH solution 4.02L down, adjust pH to 7.85, centrifugal, get supernatant 415L, stir adding 3M HCl solution 1.02L down, adjust pH to 6.72, in 85 ℃ of heat treatments, centrifugal, get concentrated solution 103.5L in 65 ℃ of concentrating under reduced pressure, ultrafiltration relative molecular weight cutoff value is 6000 dalton, gets 100.5L calf blood protein-removed extraction solution for standby; Take by weighing sodium chloride 6750g again, add the about 225L of injection water, stirring and dissolving is made into sodium chloride solution, adds the 225g active carbon, stirs evenly, and is heated to boiling, filtering decarbonization, and it is standby that fine straining gets concentrated sodium chloride solution; The 224.64L concentrated sodium chloride solution is mixed in 9.36L calf blood protein-removed extraction solution, add water for injection to 780L, stir evenly, make the calf blood protein-removed extraction transfusion, its character is little yellow or faint yellow clear liquid.Its packing specification is 250ml: contain 30mg aminoacid; 18.75mg total nitrogen; 2.25g osmotic pressure regulator.This product is used for intravenous drip, and drip velocity is 2ml/min, to the brain ischemic lesions: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; Brain function is not reached brain dementia entirely: 1 time on the one, a 250ml, vein slowly instils, and two weeks were a course of treatment; To artery vessel disease: 1 time on the one, a 250ml, vein slowly instils, and is a course of treatment all around.
Embodiment 5:
Above-mentioned calf blood protein-removed extraction transfusion, described osmotic pressure regulator can be glucose, sodium chloride, mannitol.
Claims (4)
1. the preparation technology of calf blood protein-removed extraction transfusion, it is characterized in that: get calf blood protein-removed extraction solution and osmotic pressure regulator and preparation by weight, get parts by weight and be 16500～24800 calf to, filter, learn from else's experience calf blood 16000～24000 after filtering, adjust pH is to alkalescence, centrifugal, parts by weight are 15000～23000 supernatant, adjust pH to 7.5～8, centrifugal, get parts by weight and be 12000～20000 supernatant, adjust pH to 6.5～7.5, in 70 ℃～90 ℃ heat treatments, centrifugal, 60 ℃～80 ℃ decompressed concentrates, it is 3000～5000 concentrated solution that concentrating under reduced pressure gets parts by weight, ultrafiltration, molecular cut off is 5000～10000 dalton relatively, make parts by weight and be 2500～4500 calf blood protein-removed extraction solution, get osmotic pressure regulator 50000～100000 by weight, add 20～40 times of water dissolutioies and get osmotic pressure regulator solution, add 0.01～5% active carbon again, heating, filter dense osmotic pressure regulator solution, again above-mentioned described dense osmotic pressure regulator solution is mixed with calf blood protein-removed extraction solution, add the water for injection of 70～140 times of amounts, stir evenly, make the calf blood protein-removed extraction transfusion, goods are little yellow or faint yellow clear liquid.
2. the preparation technology of calf blood protein-removed extraction transfusion according to claim 1, it is characterized in that: the preparation technology of described dense osmotic pressure regulator solution is: get parts by weight and be 50000～100000 osmotic pressure regulator, the water for injection that adds 20～40 times of amounts, stirring and dissolving is made into osmotic pressure regulator solution, adds 0.01～5% active carbon again in described solution, stir evenly, be heated to boiling, filtering decarbonization, fine straining get dense osmotic pressure regulator solution.
3. the calf blood protein-removed extraction transfusion of a claim 1 or 2 described preparation technologies' preparations, its composition comprises: calf blood protein-removed extraction and osmotic pressure regulator, it is characterized in that: described calf blood protein-removed extraction is to extract from calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, aminoacid, its parts by weight are aminoacid 20.0～30.0, total nitrogen 12.5～18.75, osmotic pressure regulator 50～100, pH value should be 5.5～8.0, and this product is little yellow or faint yellow clear liquid.
4. calf blood protein-removed extraction transfusion according to claim 3, it is characterized in that: described osmotic pressure regulator is glucose or sodium chloride or mannitol.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100136436A CN1251689C (en) | 2004-03-25 | 2004-03-25 | Veal-blood protein-removed extract infusion and its preparation process |
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| CNB2004100136436A CN1251689C (en) | 2004-03-25 | 2004-03-25 | Veal-blood protein-removed extract infusion and its preparation process |
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| CN1251689C true CN1251689C (en) | 2006-04-19 |
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| CN101433553B (en) * | 2007-12-07 | 2011-08-17 | 吉林康乃尔药业有限公司 | Method for preparing deproteinized extract of calf blood and freeze-dried powder thereof |
| CN102908363B (en) * | 2012-11-05 | 2014-01-08 | 北华大学 | Deproteinized calf blood extract and preparation process thereof |
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Assignee: Heilongjiang Jiang Shi Pharmaceutical Co.,Ltd. Assignor: Jiang Weishi Contract fulfillment period: 2008.8.18 to 2018.8.18 Contract record no.: 2008230000025 Denomination of invention: Veal-blood protein-removed extract infusion and its preparation process Granted publication date: 20060419 License type: Exclusive license Record date: 20081009 |
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