CN1251388A - Micro-capsule peridium growth regulator - Google Patents

Micro-capsule peridium growth regulator Download PDF

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Publication number
CN1251388A
CN1251388A CN99112136A CN99112136A CN1251388A CN 1251388 A CN1251388 A CN 1251388A CN 99112136 A CN99112136 A CN 99112136A CN 99112136 A CN99112136 A CN 99112136A CN 1251388 A CN1251388 A CN 1251388A
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CN
China
Prior art keywords
capsule
microcapsule
acid bacteria
lactic acid
coated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN99112136A
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Chinese (zh)
Inventor
常维山
丁庆华
李祥明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Feed Supervision Institute Of Shandong Province
Shandong Agricultural University
Original Assignee
Feed Supervision Institute Of Shandong Province
Shandong Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Feed Supervision Institute Of Shandong Province, Shandong Agricultural University filed Critical Feed Supervision Institute Of Shandong Province
Priority to CN99112136A priority Critical patent/CN1251388A/en
Publication of CN1251388A publication Critical patent/CN1251388A/en
Pending legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Dairy Products (AREA)

Abstract

The microcapsule coated prebiotics is characterized by that it utilizes geltine and gum arabic as capsule material, uses lactic acid bacteria as capsule core, regulates pH value of coating liquor to make capsule material coat capsule core to form double-layer capsule structure, then uses formaldehyde to fix for a short time, regulates pH value to neutrality and makes centrifugal separation of microcapsule and supernatant, fluid, adds starch and dries to form the invented product. Under the condition of room temp. the lactic acid bateria coated with microcapsule can live above half year, and the application effect of prebiotics made of lactic acid bacteria coated with microcapsule can be obviously raised.

Description

Micro-capsule peridium growth regulator
The present invention is a fodder additives.
This product utilization gelatin and gum arabic are the capsule material, are capsule-core with the milk-acid bacteria, are the probiotics of solidifying agent preparation becoming bilayer vesicles bag quilt with formaldehyde.
Milk-acid bacteria is a class anaerobism or a microaerobe, and is dead easily under natural atmosphere, drying and room temperature storage condition, and the validity period of probiotics (by preparations such as milk-acid bacterias) is shortened.
This technology is wrapped in capsule central authorities by the preparation bilayered microcapsule with milk-acid bacteria, and is isolated with air, increased the resistibility of milk-acid bacteria to oxygen, drying, temperature.
The method of this product is:
1. after the heating of gelatin and gum arabic being dissolved, join in 40~45 ℃ the warm distilled water, the final concentration of gelatin is 3% (W/V), and the final concentration of gum arabic is 1% (W/V).
2. according to 1/10 amount of volume, add lactobacillus suspension.
3. with acetic acid above-mentioned mixed solution is transferred to pH4.0~4.5 while stirring.
4. the distilled water that adds 3~4 times 37~40 ℃.
5. aforesaid liquid is reduced to 10~15 ℃.
6. adding formaldehyde solution to final concentration is 1%, acts on 1 hour.
7. the hydro-oxidation sodium solution transfers to pH7.0~7.2 with aforesaid liquid.
8. 1,000 rev/min, centrifugal 10~15 minutes, abandon supernatant, throw out is admixed in the stone flour 40~45 ℃ of oven dry.
The advantage of this technology is the coating rate height, and cost is low, and is effective.
9. the flow process of this product is as follows:
Lactobacillus suspension → gelatin gum arabic mixed solution → adjusting pH to 4.0~4.5 → adding distilled water → encystation → adding formaldehyde solution → adjusting pH to 7.0~7.2 → centrifugal → throw out mixes with stone flour → dry → add auxiliary material → packing → warehouse-in.
The embodiment of the invention:
Get 100 milliliters of lactic acid bacteria culture solutions, join in 900 milliliters 42 ℃ the solution that contains 3% gelatin, 1% gum arabic, add acetum while stirring.PH transfers to 4.0 with liquid, adds 3 liters of 37 ℃ of distilled water, is cooled to 15 ℃ naturally, adds 40 milliliters of formaldehyde solutions, after 1 hour 1,000 rev/min centrifugal 15 minutes, throw out is mixed with 0.5 kilogram of starch, 40 ℃ the oven dry 2 hours.
Behind aforesaid operations, milk-acid bacteria will be encapsulated in the bilayered microcapsule of true footpath 10~100nm.

Claims (4)

1. micro-capsule peridium growth regulator.Utilize gelatin, gum arabic as the capsule material, milk-acid bacteria (plant lactobacillus and bifidus bacillus) is a capsule-core, by regulating potential of hydrogen and formaldehyde fixed, makes Nang Caibao be become microcapsule by capsule-core.It is characterized in that milk-acid bacteria is wrapped in the bilayered microcapsule of diameter 10-100 micron.
2. microcapsule method for coating according to claim 1 is characterized in that the ratio of lactobacillus suspension and capsule material coating buffer is 5-10: 1.
3. microcapsule method for coating according to claim 1 is characterized in that the formaldehyde fixed time is 1-2 hour.
4. microcapsule method for coating according to claim 1 is characterized in that utilizing the microcapsule diameter of this prepared to be the 10-100 micron.
CN99112136A 1999-03-22 1999-03-22 Micro-capsule peridium growth regulator Pending CN1251388A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN99112136A CN1251388A (en) 1999-03-22 1999-03-22 Micro-capsule peridium growth regulator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN99112136A CN1251388A (en) 1999-03-22 1999-03-22 Micro-capsule peridium growth regulator

Publications (1)

Publication Number Publication Date
CN1251388A true CN1251388A (en) 2000-04-26

Family

ID=5275469

Family Applications (1)

Application Number Title Priority Date Filing Date
CN99112136A Pending CN1251388A (en) 1999-03-22 1999-03-22 Micro-capsule peridium growth regulator

Country Status (1)

Country Link
CN (1) CN1251388A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1534307A4 (en) * 2002-06-11 2007-09-05 Advanced Bionutrition Corp Zooplankton enrichment with probionts and prebionts and uses thereof
CN102068947A (en) * 2010-12-28 2011-05-25 上海工程技术大学 Preparation method of N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide microcapsules
CN102302160A (en) * 2011-09-07 2012-01-04 黑龙江省乳品工业技术开发中心 Preparation method of Bifidobacterium infantis microcapsules
CN101249109B (en) * 2008-03-14 2012-06-27 华侨大学 Lactobacillus reuteri hollow micro-capsule and its preparing method
CN103798793A (en) * 2013-12-30 2014-05-21 青岛碧水蓝天生物技术有限公司 Lactobacillus powder containing ocean fishskin collagen peptide

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1534307A4 (en) * 2002-06-11 2007-09-05 Advanced Bionutrition Corp Zooplankton enrichment with probionts and prebionts and uses thereof
CN101249109B (en) * 2008-03-14 2012-06-27 华侨大学 Lactobacillus reuteri hollow micro-capsule and its preparing method
CN102068947A (en) * 2010-12-28 2011-05-25 上海工程技术大学 Preparation method of N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide microcapsules
CN102302160A (en) * 2011-09-07 2012-01-04 黑龙江省乳品工业技术开发中心 Preparation method of Bifidobacterium infantis microcapsules
CN102302160B (en) * 2011-09-07 2013-03-27 黑龙江省乳品工业技术开发中心 Preparation method of Bifidobacterium infantis microcapsules
CN103798793A (en) * 2013-12-30 2014-05-21 青岛碧水蓝天生物技术有限公司 Lactobacillus powder containing ocean fishskin collagen peptide

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