CN1245811A - Method for extracting soybean saponin from deffated soybean cake or soybean chips - Google Patents

Abstract

The present invention relates to a method for extracting soybean saponin from defatted soybean cake. Said method includes the following steps: using defatted soybean cake as raw material, adopting ethyl alcohol extraction and n-butanol and water phase redistribution to obtain crude saponin, then making the obtained crude saponin undergo the processes of macroporous resin column adsorption, water and ethyl alcohol elution, concentration and spray-drying so as to obtain the soybean saponin powder. Said invention is simple and convenient in technological process, low in cost, can effectively remove protein and polysaccharides, raise purity of saponin, and can be extensively used in the fields of light industry, food and pharmaceutic industry.

Description

From the skimmed soy beans dregs of beans, extract the method for Soyasaponin

The invention belongs to the extraction process of Soyasaponin.

Soyasaponin is a kind of pentacyclic triterpene chemical substance that is present in the leguminous plants, has physiologically actives such as anti-oxidant, Green Tea Extract, and very high health-care effect and pharmaceutical use are arranged, and therefore, is widely used in light industry, food and medicine industry.Extract the saponin ways that adopt at present is with methyl alcohol or ethanol-water extraction more, propyl carbinol: the water reallocation makes.The saponin purity that makes like this is low, and is difficult for drying, and solvent-oil ratio is big, is difficult to realize suitability for industrialized production.

The objective of the invention is to overcome the deficiencies in the prior art, a kind of technology of extracting Soyasaponin from the skimmed soy beans dregs of beans is provided, the saponin purity height of extraction, cost is low, can realize suitability for industrialized production.

Technical scheme of the present invention realizes as follows:

A, be raw material, it is pulverized, add 85% ethanolic soln dipping and spend the night with the skimmed soy beans dregs of beans;

B, be heated to boiling, lixiviate 3-5 hour, extract 3 times, merge extract just, to filter, reduced vacuum is concentrated near doing, and obtains stiff paste form;

C, stiff paste form is dissolved in water, adds propyl carbinol, all mix, leave standstill, after the layering, divide and take out n-butanol layer, remaining water layer continues to add propyl carbinol, again through all mix, leave standstill, after the layering, divide to take out n-butanol layer again, double, the n-butanol layer of obtaining for three times is merged, be evaporated to driedly, obtain thick saponin;

D, thick saponin is added less water dissolving, be added on the macroporous resin adsorption post, with the ethanol elution of water, Diluted Alcohol and 85%, discard water layer and Diluted Alcohol layer successively, collect the elutriant that contains saponin;

E, the elutriant reduction vaporization that will contain saponin are closely dried, the spray-dried again Soyasaponin powder that makes.

The invention has the advantages that this technology extraction Soyasaponin, the resin absorption that is adopted, wash-out can effectively be removed protein and polyose in the soybean, has improved the purity of saponin, and dna purity can reach more than 70%, can create favorable economic benefit.Make other food or medicine with it, anti-curing cancers, preventing cardiovascular disease etc. are had good booster action.

Provide specific embodiment below:

Take by weighing skimmed soy beans dregs of beans 250g, pulverizing is the meal shape, adds the 1000ml85% ethanolic soln.In the refluxing extraction jar, soak, after 12 hours, be heated to boiling, extracted 5 hours, after the cooling, leach extract just, put into the ethanol of 750ml 85% in the residue again, continued heating and refluxing extraction 5 hours, double, merge extract just, reduction vaporization is removed the ethanol in the first extract, obtains to contain the paste of saponin.In paste, add 200ml water again, the propyl carbinol of 1000ml, vibration mixed 5 minutes, and static back branch is got n-butanol layer, uses with the quadrat method separated secondary again, merges propyl carbinol liquid, and it is evaporated near doing, and obtains the thick saponin of soybean.Dissolve thick saponin with less water, put into the macroporous resin column that in advance fills, with the 30% ethanol drip washing of 500ml water, 200ml, discard leacheate, earlier succeeded by the ethanol of 500ml50%, 85% ethanol of 1000ml, wash-out successively, merge elutriant, concentrating under reduced pressure is removed ethanol, after the cooling, the extract spraying drying is made dry powder, can obtain Soyasaponin dry powder 10-12g.

Claims (1)

1, from the skimmed soy beans dregs of beans, extract the method for Soyasaponin, comprise that water or organic solvent extraction separate, and is characterized in that:

A, be raw material, it is pulverized, add 85% ethanolic soln dipping and spend the night with the skimmed soy beans dregs of beans;

B, be heated to boiling, lixiviate 3-5 hour, extract 3 times, merge extract just, to filter, reduced vacuum is concentrated near doing, and obtains stiff paste form;

C, stiff paste form is dissolved in water, adds propyl carbinol, all mix, leave standstill, after the layering, divide and take out n-butanol layer, remaining water layer continues to add propyl carbinol, again through all mix, leave standstill, after the layering, divide to take out n-butanol layer again, double, the n-butanol layer of obtaining for three times is merged, be evaporated to driedly, obtain thick saponin;

D, thick saponin is added less water dissolving, be added on the macroporous resin adsorption post, with the ethanol elution of water, Diluted Alcohol and 85%, discard water layer and Diluted Alcohol layer successively, collect the elutriant that contains saponin;

E, the elutriant reduction vaporization that will contain saponin are closely dried, the spray-dried again Soyasaponin powder that makes.