CN1245623C - Lentinan molecular weight and molecular weight distribution measuring method - Google Patents
Lentinan molecular weight and molecular weight distribution measuring method Download PDFInfo
- Publication number
- CN1245623C CN1245623C CN 03112908 CN03112908A CN1245623C CN 1245623 C CN1245623 C CN 1245623C CN 03112908 CN03112908 CN 03112908 CN 03112908 A CN03112908 A CN 03112908A CN 1245623 C CN1245623 C CN 1245623C
- Authority
- CN
- China
- Prior art keywords
- molecular weight
- lentinan
- hpgpc
- determination method
- distribution determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Abstract
Description
C | Lentinan (Lentinan) | Japan produces lentinan | Resolve |
1 | 104.6 | 104.6 | β-D-(1 → 3) glucose |
2 | 74.5 | 74.3 | β-D-(1 → 3) glucose |
3 | 87.7 | 87.7 | β-D-(1 → 3) glucose |
4 | 70.2 | 70.2 | β-D-(1 → 3) glucose |
5 | 77.8 | 77.9 | β-D-(1 → 3) glucose |
6 | 62.5 | 62.5 | β-D-(1 → 3) glucose |
1′ | 104.9 | 104.6 | β-D-(1 → 6) glucose |
6′ | 71.5 | 71.5 | β-D-(1 → 6) glucose |
Number of times | Phosphate buffer pH value | K | α |
1 | 7.89 | 0.0540 | 0.605 |
2 | 7.91 | 0.0538 | 0.607 |
3 | 7.93 | 0.0540 | 0.606 |
4 | 7.86 | 0.0538 | 0.607 |
5 | 7.88 | 0.0541 | 0.609 |
6 | 7.90 | 0.0536 | 0.604 |
7 | 7.84 | 0.0535 | 0.609 |
8 | 7.92 | 0.0537 | 0.610 |
9 | 7.87 | 0.0539 | 0.609 |
10 | 7.90 | 0.0537 | 0.609 |
11 | 7.91 | 0.0540 | 0.608 |
12 | 7.89 | 0.0537 | 0.609 |
13 | 7.90 | 0.0536 | 0.610 |
14 | 7.86 | 0.0536 | 0.608 |
15 | 7.94 | 0.0540 | 0.608 |
16 | 7.97 | 0.0542 | 0.607 |
17 | 7.84 | 0.0538 | 0.605 |
18 | 7.92 | 0.0540 | 0.605 |
19 | 7.90 | 0.0538 | 0.602 |
20 | 7.95 | 0.0540 | 0.608 |
21 | 7.81 | 0.0543 | 0.608 |
22 | 7.97 | 0.0538 | 0.607 |
23 | 7.87 | 0.0538 | 0.610 |
24 | 7.95 | 0.0537 | 0.606 |
25 | 7.89 | 0.0540 | 0.607 |
Mean value | 7.90 | 0.0539 | 0.607 |
Standard deviation | 0.0404 | 0.000200 | 0.00201 |
# | Cumulative% (%) | Mol Wt (Daltons) | Retention Time (min) |
1 | 0.061 | 1707673 | 33.333 |
2 | 0.449 | 1496554 | 34.140 |
3 | 3.015 | 1311536 | 34.948 |
4 | 9.061 | 1149391 | 35.755 |
5 | 15.813 | 1007293 | 36.562 |
6 | 21.926 | 882762 | 37.370 |
7 | 27.394 | 773626 | 38.178 |
8 | 32.402 | 677983 | 38.985 |
9 | 37.043 | 594164 | 39.792 |
10 | 41.387 | 520708 | 40.600 |
11 | 45.514 | 456333 | 41.407 |
12 | 49.470 | 399917 | 42.215 |
13 | 53.210 | 350476 | 43.022 |
14 | 56.786 | 307146 | 43.830 |
15 | 60.219 | 269174 | 44.637 |
16 | 63.554 | 235896 | 45.445 |
17 | 66.759 | 206732 | 46.252 |
18 | 69.813 | 181174 | 47.060 |
19 | 72.661 | 158776 | 47.867 |
20 | 75.277 | 139146 | 48.675 |
21 | 77.721 | 121944 | 49.482 |
22 | 79.946 | 106868 | 50.290 |
23 | 82.033 | 93656 | 51.097 |
24 | 83.981 | 82077 | 51.905 |
25 | 85.793 | 71930 | 52.712 |
26 | 87.524 | 63037 | 53.520 |
27 | 89.191 | 55244 | 54.327 |
28 | 90.757 | 48414 | 55.135 |
29 | 92.273 | 42429 | 55.942 |
30 | 93.713 | 37183 | 56.750 |
31 | 94.999 | 32586 | 57.557 |
32 | 96.067 | 28558 | 58.365 |
33 | 96.971 | 25027 | 59.172 |
34 | 97.721 | 21933 | 59.980 |
35 | 98.338 | 19222 | 60.787 |
36 | 98.839 | 16845 | 61.595 |
37 | 99.277 | 14763 | 62.402 |
38 | 99.624 | 12938 | 63.210 |
39 | 99.875 | 11338 | 64.017 |
40 | 100.000 | 9936 | 64.825 |
The raw material lot number | Weight-average molecular weight (Mw) (unit: ten thousand) | Relative deviation (%) | |
The HPGPC method | Light scattering method | ||
1 | 60.0 | 57.5 | 2.1 |
2 | 50.2 | 53.0 | 2.7 |
3 | 70.4 | 71.8 | 1.0 |
4 | 56.6 | 61.0 | 3.7 |
5 | 57.7 | 59.7 | 1.7 |
6 | 54.9 | 52.2 | 2.5 |
Claims (13)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03112908 CN1245623C (en) | 2003-03-04 | 2003-03-04 | Lentinan molecular weight and molecular weight distribution measuring method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03112908 CN1245623C (en) | 2003-03-04 | 2003-03-04 | Lentinan molecular weight and molecular weight distribution measuring method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1437019A CN1437019A (en) | 2003-08-20 |
CN1245623C true CN1245623C (en) | 2006-03-15 |
Family
ID=27634169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 03112908 Expired - Lifetime CN1245623C (en) | 2003-03-04 | 2003-03-04 | Lentinan molecular weight and molecular weight distribution measuring method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1245623C (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7650152B2 (en) | 2000-12-15 | 2010-01-19 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US7933244B2 (en) | 2000-12-15 | 2011-04-26 | Adaptix, Inc. | Multi-carrier communications with group-based subcarrier allocation |
US8760992B2 (en) | 2004-12-07 | 2014-06-24 | Adaptix, Inc. | Method and system for switching antenna and channel assignments in broadband wireless networks |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101039964B (en) * | 2004-08-12 | 2012-11-14 | 利普生技术有限公司 | Fractionation of charged polysaccharide |
CN101059483B (en) * | 2007-05-25 | 2010-11-24 | 南京生命能科技开发有限公司 | Sugar-ferric hydroxide composite molecular weight and molecular weight distribution determination method |
CN101441202B (en) * | 2008-12-29 | 2011-08-10 | 河南工业大学 | Method for detecting fungal polysaccharide |
CN101852782B (en) * | 2009-04-02 | 2011-11-30 | 鲁南制药集团股份有限公司 | Method for measuring impurity content of faropenem polymers in faropenem sodium raw materials and preparations |
US8195405B2 (en) * | 2009-06-30 | 2012-06-05 | Wyatt Technology Corporation | Method for characterizing reversible association of macromolecules at high concentration |
CN102262067A (en) * | 2010-05-31 | 2011-11-30 | 北京化工大学 | Attenuate total reflection infrared spectrometry for fast detection of iodine value of edible oil |
CA2848520C (en) | 2011-09-29 | 2019-11-26 | Seattle Genetics, Inc. | Intact mass determination of protein conjugated agent compounds |
CN102590408B (en) * | 2012-01-04 | 2014-07-09 | 亚宝药业集团股份有限公司 | Method for detecting macromolecular substances in perhexiline injection |
CN103954716A (en) * | 2014-04-24 | 2014-07-30 | 上海慈瑞医药科技有限公司 | Method for measuring molecular weight and molecular weight distribution of lentinan |
CN105203482B (en) * | 2015-09-23 | 2018-08-07 | 西南石油大学 | The assay method of hydrophobic associated polymer molecular weight distribution curve |
CN106198594B (en) * | 2016-06-20 | 2018-07-27 | 中国科学院长春应用化学研究所 | A kind of characterizing method of polymer molecular weight |
CN109459523B (en) | 2017-09-06 | 2022-11-08 | 上海绿谷制药有限公司 | Method for measuring weight average molecular weight and content of acidic sugar soluble salt |
CN109459505B (en) * | 2017-09-06 | 2023-04-25 | 绿谷(上海)医药科技有限公司 | Method for measuring weight average molecular weight and content of mannuronic acid substances |
CN109682893A (en) * | 2017-10-19 | 2019-04-26 | 上海慈瑞通鑫医药技术有限公司 | The measuring method of lentinan content in lentinan composition |
CN109682903A (en) * | 2019-02-19 | 2019-04-26 | 天津赛诺制药有限公司 | A kind of detection method of astragalus root polysaccharide molecular weight |
CN113281443A (en) * | 2021-06-07 | 2021-08-20 | 广州广电计量检测股份有限公司 | Polysaccharide detection method |
-
2003
- 2003-03-04 CN CN 03112908 patent/CN1245623C/en not_active Expired - Lifetime
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8964719B2 (en) | 2000-12-15 | 2015-02-24 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8738020B2 (en) | 2000-12-15 | 2014-05-27 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US9344211B2 (en) | 2000-12-15 | 2016-05-17 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8934445B2 (en) | 2000-12-15 | 2015-01-13 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US8891414B2 (en) | 2000-12-15 | 2014-11-18 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US8743729B2 (en) | 2000-12-15 | 2014-06-03 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US8743717B2 (en) | 2000-12-15 | 2014-06-03 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US8750238B2 (en) | 2000-12-15 | 2014-06-10 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US9219572B2 (en) | 2000-12-15 | 2015-12-22 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8767702B2 (en) | 2000-12-15 | 2014-07-01 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US7933244B2 (en) | 2000-12-15 | 2011-04-26 | Adaptix, Inc. | Multi-carrier communications with group-based subcarrier allocation |
US7715358B2 (en) | 2000-12-15 | 2010-05-11 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8036199B2 (en) | 2000-12-15 | 2011-10-11 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8934375B2 (en) | 2000-12-15 | 2015-01-13 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8958386B2 (en) | 2000-12-15 | 2015-02-17 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US7650152B2 (en) | 2000-12-15 | 2010-01-19 | Adaptix, Inc. | Multi-carrier communications with adaptive cluster configuration and switching |
US9191138B2 (en) | 2000-12-15 | 2015-11-17 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US9203553B1 (en) | 2000-12-15 | 2015-12-01 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US9210708B1 (en) | 2000-12-15 | 2015-12-08 | Adaptix, Inc. | OFDMA with adaptive subcarrier-cluster configuration and selective loading |
US8760992B2 (en) | 2004-12-07 | 2014-06-24 | Adaptix, Inc. | Method and system for switching antenna and channel assignments in broadband wireless networks |
US8797970B2 (en) | 2004-12-07 | 2014-08-05 | Adaptix, Inc. | Method and system for switching antenna and channel assignments in broadband wireless networks |
Also Published As
Publication number | Publication date |
---|---|
CN1437019A (en) | 2003-08-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1245623C (en) | Lentinan molecular weight and molecular weight distribution measuring method | |
Kitamura et al. | An antitumor, branched (1→ 3)-β-D-glucan from a water extract of fruiting bodies of Cryptoporus volvatus | |
Restaino et al. | A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate | |
CN101493446B (en) | Method for measuring free polyethyleneglycol content in sample or products | |
CN109682901A (en) | The average degree of modification measuring method of Pegylation protein medicaments | |
CN109580428A (en) | A kind of method of hyaluronan molecule amount in simplicity Accurate Determining solution | |
CN1693890A (en) | GC/MS inner scaling detection method for simultaneous investigating residual multiple organophosphorus in food | |
CN1880344A (en) | Low molecular weight heparin and its preparation method | |
CN110736796B (en) | Method for detecting molecular weight of pneumococcal capsular polysaccharide | |
CN1945313A (en) | Method for detecting lentinan molecular weight and molecular weight distribution | |
CN111289666A (en) | Construction method of lentinan multivariate quantitative fingerprint spectrum | |
JP6633828B2 (en) | Method and apparatus for measuring polysaccharides | |
CN101059483A (en) | Sugar-ferric hydroxide composite molecular weight and molecular weight distribution determination method | |
CN109991067A (en) | A kind of accurate method for detecting the gastrodia elata polysaccharide radius of gyration and molecular weight distribution | |
CN102590408B (en) | Method for detecting macromolecular substances in perhexiline injection | |
CN107561173B (en) | Method for measuring high-molecular polymer in Ropeinan sodium powder injection | |
CN103323541A (en) | Quality detection method for heparin sodium tube-enveloping injection | |
CN109459503B (en) | Method for measuring weight average molecular weight and content of heparin drugs | |
CN1217191C (en) | Homologous system model method for quantitatively detecting bacterial endotoxin of blood | |
CN112986457A (en) | Method for detecting polysaccharide by HPSEC-MALS method and correlating polysaccharide with Sepharose CL-4B method | |
Kwon et al. | Enantiomeric separation of some flavanones using shinorhizobial linear octasaccharides in CE | |
CN101062031A (en) | Method of controlling the quality of silymarin oral aolid preparation | |
CN108226330A (en) | A kind of detection method of pachymaran molecular weight and molecular weight distribution | |
RU2295127C1 (en) | Determination of polymer chitosan molecules in chitosan preparations | |
Buzzega et al. | Fluorophore‐assisted carbohydrate electrophoresis for the determination of molecular mass of heparins and low‐molecular‐weight (LMW) heparins |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: NANJING KANGHAI PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: NANJING ZHENZHONG BIOLOGICAL ENGINEERING CO., LTD. Effective date: 20040625 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20040625 Address after: 210061 hi tech Industrial Development Zone, Jiangsu, Nanjing Applicant after: Kanghai Pharmaceutical Co.,Ltd. Nanjing Address before: 210061 hi tech Industrial Development Zone, Jiangsu, Nanjing Applicant before: Nanjing Zhenzhong Bioengineering Co.,Ltd. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: NANJING SIKE PHARMA CO., LTD. Free format text: FORMER OWNER: NANJING KANGHAI PHARMACEUTICAL CO., LTD. Effective date: 20101213 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C56 | Change in the name or address of the patentee |
Owner name: NANJING LVYE SIKE PHARMA CO., LTD. Free format text: FORMER NAME: NANJING SIKE PHARMA CO., LTD. |
|
CP03 | Change of name, title or address |
Address after: High road 210061 Jiangsu city of Nanjing province high tech Industrial Development Zone No. 28 Patentee after: NANJING LVYE PHARMACEUTICAL CO.,LTD. Address before: 210061 hi tech Industrial Development Zone, Jiangsu, Nanjing Patentee before: Nanjing Cike Pharmaceutical Co.,Ltd. |
|
TR01 | Transfer of patent right |
Effective date of registration: 20101213 Address after: 210061 hi tech Industrial Development Zone, Jiangsu, Nanjing Patentee after: Nanjing Cike Pharmaceutical Co.,Ltd. Address before: 210061 hi tech Industrial Development Zone, Jiangsu, Nanjing Patentee before: Kanghai Pharmaceutical Co.,Ltd. Nanjing |
|
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Kanghai Pharmaceutical Co.,Ltd. Nanjing Assignor: NANJING LVYE PHARMACEUTICAL CO.,LTD. Contract record no.: 2010320001161 Denomination of invention: Lentinan molecular weight and molecular weight distribution measuring method Granted publication date: 20060315 License type: Exclusive License Open date: 20030820 Record date: 20101028 |
|
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: NANJING LVYE PHARMACEUTICAL CO.,LTD. Assignor: Kanghai Pharmaceutical Co.,Ltd. Nanjing Contract record no.: 2010320001161 Denomination of invention: Lentinan molecular weight and molecular weight distribution measuring method Granted publication date: 20060315 License type: Exclusive License Open date: 20030820 Record date: 20101028 |
|
C56 | Change in the name or address of the patentee | ||
CP01 | Change in the name or title of a patent holder |
Address after: High road 210061 Jiangsu city of Nanjing province high tech Industrial Development Zone No. 28 Patentee after: NANJING LUYE PHARMACEUTICAL Co.,Ltd. Address before: High road 210061 Jiangsu city of Nanjing province high tech Industrial Development Zone No. 28 Patentee before: NANJING LVYE PHARMACEUTICAL CO.,LTD. |
|
CX01 | Expiry of patent term | ||
CX01 | Expiry of patent term |
Granted publication date: 20060315 |