CN1244685C - Acid resistant mutagenic bacteria and its dump leaching process used for ore - Google Patents
Acid resistant mutagenic bacteria and its dump leaching process used for ore Download PDFInfo
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- CN1244685C CN1244685C CNB021288313A CN02128831A CN1244685C CN 1244685 C CN1244685 C CN 1244685C CN B021288313 A CNB021288313 A CN B021288313A CN 02128831 A CN02128831 A CN 02128831A CN 1244685 C CN1244685 C CN 1244685C
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- acid resistant
- ore
- resistant mutagenic
- heap
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Abstract
The present invention provides an acid resistant mutagenic bacterium and technology of using the acid resistant mutagenic bacterium for the heap leaching of ore. The acid resistant mutagenic bacterium is a mixed bacterium of ferrous oxide thiobacilli, sulfur oxide thiobacilli and ferrous oxide micro spirilla and is named as Thiobacillusferrooxidans. Retech. III. The registration number of preservation is CCTCC. No: M200033, and the preservation unit is the National Type Culture Preservation Center of Chine and is positioned in Wuhan university. The technology of using the acid resistant mutagenic bacteria for heap leaching comprises: the acid resistant mutagenic mixed bacteria are acclimated and cultured by enlarging, and ore is heaped; then, the acid resistant mutagenic mixed bacteria are sprayed or dripped on the ore, and leach solution obtained in a leaching procedure is sent to a metal recovering procedure. The present invention has the advantages that the acid resistant mutagenic bacterium can work in an environment with a pH value between 0.9 and 3.0 and is used for the heap leaching of ore, the leaching cycle is shortened by 1/3 to 2/3, the leaching rate is enhanced by 10 to 30%, and the production cost is reduced by 10 to 30%.
Description
Technical field
The present invention relates to a kind of acid resistant mutagenic bacteria and be used for the heap leaching method of ore, the mixed bacterium of particularly a kind of acid resistant mutagenic thiobacillus ferrooxidant, thiobacillus thiooxidans and the little spirillum of iron protoxide etc. and utilize this bacterium from ore or concentrate or mine tailing, to extract the biological dump leaching process of metal.
Background technology
The existing domestication bacterium that is used for biological dump leaching and extracting metal, its leaching velocity is slow, and leaching cycle is long, and when low pH (pH＜1.5), bacterial activity is not high, thereby influences metal leaching rate, soaks the ore deposit poor performance, therefore is necessary to provide a kind of acid resistant mutagenic bacteria.
Summary of the invention
The purpose of this invention is to provide a kind of acid resistant mutagenic bacteria and be used for the heap leaching method of ore, this technology is compared with existing technology, and leaching cycle shortens 1/3-2/3, and leaching yield improves 10-30%, and production cost reduces 10-30%.
For achieving the above object, the present invention takes following design: used acid resistant mutagenic bacteria is a thiobacillus ferrooxidant among the present invention, thiobacillus thiooxidans, the acid resistant mutagenic mixed bacterium of the little spirillum of iron protoxide, (this bacterium is deposited in the Wuhan University of China national typical case culture collection center, name is called Thiobacillusferrooxidans Retech III, preservation registration number CCTCC No:M200033) after obtaining this bacterial classification, cultivate for the ore that adds 2-9K iron-free substratum and required leaching in the ordinary tap water of 1.0-1.5 or concentrate or mine tailing carry out the domestication of ore-leaching bacteria adaptability in the pH value, amplification culture step by step, wherein bacterial load is the 10-20% of culture cumulative volume, the breeze amount that adds is the 5-10% of culture gross weight, and obtaining bacteria concentration is 10
7-10
9The ore deposit bacterium is soaked in the domestication of the adaptability of individual/ml, and its growth temperature is at 4～45 ℃, pH between 0.9～3.0, optimum growh pH1.2-1.7, this bacterium is used further to the ore that carries out subsequently or the biological dump leaching of concentrate or mine tailing.
The mutafacient system of above-mentioned acid resistant mutagenic bacteria mixed bacterium is the acidic mine water water sample that collection is contained thiobacillus ferrooxidant, thiobacillus thiooxidans and the little spirillum of iron protoxide, in the 9K of pH1.7-2.0 basic medium, separate, cultivate, obtain to contain thiobacillus ferrooxidant, bacteria concentration is 10
8Individual/purer bacterial classification more than the ml, this bacterial classification is tamed in containing the 9K substratum that must soak fine ore and is cultivated into the domestication mixed bacterium, again through He-Ne laser apparatus irradiation mutagenesis, obtain mutant strain, obtain anti-low pH value mutagenesis bacterium through screening again, this mutagenesis bacterium is soaked the adaptability domestication and cultivates the acquisition acid resistant mutagenic bacteria. and this bacterium can will be cultivated in 12 hours and contain 44.43g/Lfe in the bacterium
2+Ion complete oxidation (100%) is Fe
3+Ion, the composition of minimum medium 9K: (NH
4)
2SO
43g/L, KCl 0.1g/L, K
2HPO
40.5g/L, Ca (NO
3)
20.01g/L, FeSO
47H
2O 44.43g/L.
The particle diameter of powder ore is less than 50mm.Building heap is concentrate or the mine tailing ore particle through granulation with ore particle, and its diameter is 5-25mm.Build heap and build heap limit spray quinoline or drip the solution that pouring contains acid resistant mutagenic bacteria for the limit, the solution spraying amount is the 8-20% of ore weight.
2-9K iron-free basic medium is: (NH
4)
2SO
40.6-3g/L, KCl 0.02-0.1g/L, K
2HPO
40.1-0.5g/L, Ca (NO
3)
20.01g/L, MgSO
47H
2O0.1-0.5g/L.
Description of drawings
Fig. 1 is the process frame chart of an embodiment of the present invention
Fig. 2 is the process frame chart of the another kind of embodiment of the present invention
Embodiment
As shown in Figure 1,1 is the acid resistant mutagenic mixed bacterium through preservation, it is carried out adaptability domestication and amplification culture 2, ore 3 is through broken process 4, granularity is controlled at particle diameter and is＜50mm, builds heap 5 subsequently, and the limit is built the heap limit and added and soak the ore deposit bacterium after domestication and the amplification culture, 6 for a spray quinoline or a pouring leaching, subsequently leach liquor delivered to metal recovery operation etc.; As shown in Figure 2,8 is that flotation concentrate or mine tailing are maintenance through granulation or pelletizing operation 9,10, adds subsequently through the ore deposit bacterium of soaking of domestication and amplification culture and drips pouring leaching 11, delivers to metal recovery operation 7 again,
Comparative example (its block diagram such as Fig. 1)
At first certain copper mine acidic mine water water sample that collection is contained thiobacillus ferrooxidant, thiobacillus thiooxidans and the little spirillum of iron protoxide separates, cultivates in the 9K basic medium of 1.7-2.0 in the pH value.The composition of 9K basic medium: (NH
42SO
43g/L, KCl 0.1g/L, K
2HPO
40.5g/L, Ca (NO
3)
20.01g/L, FeSO
47H
2O 44.43g/L, cultivate for the adaptability domestication that adds granularity in the ordinary tap water of 1.7-2.0 and carry out ore-leaching bacteria and amplification culture step by step in the pH value then for the low-grade copper sulfide ore of certain cupric 0.65% of＜200 orders 95%, wherein bacterial load is the 10-20% of culture cumulative volume, the mineral dust amount that adds is the 5-10% of training oxygen thing gross weight, and obtaining bacteria concentration is 10
8-10
9The ore deposit bacterium is soaked in the wild adaptability domestication of individual/ml, is used for the bacteria heap leaching of ore then.
The low-grade copper sulfide ore of certain cupric 0.65% is crushed to granularity is＜15mm, carry out ore then and build heap, pile high 4.5m, when building heap, spray the wild adaptability that it is good that adaptability is tamed amplification culture that contains that accounts for ore weight 15% and tame the spray liquid that soaks the ore deposit bacterium; Build heap and finish the back and soak the ore deposit bacterium by the wild adaptability domestication that it is good that 10% of spray liquid cumulative volume inserts the domestication of adaptability amplification culture and begin spray and leach, spraying extraction time is 3-7 days, and spray rate is 5-20L/ (m
2H), enter leisure then, leisure time is 2-7 days, and spray carries out in turn with leisure; Cu in leach liquor
2+After ionic concn reaches 1.5g/L, enter extraction-reextraction-electrodeposition, output A level cathode copper, raffinate return and spray leaching.Copper extractant is selected Lix984N for use, and thinner is selected sulfonated kerosene for use.Leached 198 days, copper leaching rate is 60.44%.
Embodiment 1 (its block diagram such as Fig. 1)
At first to containing thiobacillus ferrooxidant, the acid resistant mutagenic mixed bacterium of thiobacillus thiooxidans and the little spirillum of iron protoxide (deposit in the Wuhan University of China national typical case culture collection center by this bacterium, the culture name is called Thiobacillus ferrooxidans Retech III, preservation registration number is CCTCC No:M200033) be 1.3-1.4 in the pH value, temperature is to add in the ordinary tap water of natural temperature that 2-9K iron-free basic medium and granularity carry out for the low-grade copper sulfide ore of certain cupric 0.65% of＜200 orders 95% that the domestication of ore-leaching bacteria adaptability is cultivated and amplification culture step by step, wherein bacterial load is the 10-20% of culture cumulative volume, the mineral dust amount that adds is the 5-10% of culture gross weight, and obtaining bacteria concentration is 10
7-10
9The ore deposit bacterium is soaked in the adaptability domestication acid resistant mutagenic mixing of individual/ml, is used for the bacteria heap leaching of ore then.
The low-grade copper sulfide ore of certain cupric 0.65% is crushed to granularity is＜15mm, carry out ore then to build heap, pile high 4.5mm, spray accounts for the spray liquid that contains the good acid resistant mutagenic bacteria of adaptability domestication amplification culture of ore weight 15% when building heap; Build heap and finish the back and insert the good acid resistant mutagenic bacteria of adaptability domestication amplification culture by 10% of spray liquid cumulative volume and begin spray and leach, spraying extraction time is 3-7 days, and spray rate is 5-20L/ (m
2H), enter leisure then, leisure time is 2-7 days, and spray carries out in turn with leisure; Cu in leach liquor
2+After ionic concn reaches 1.5g/L, enter extraction-reextraction-electrodeposition, output A level cathode copper, raffinate return and spray leaching.Copper extractant is selected for use
Lix984N,Thinner is selected sulfonated kerosene for use.Leached 53 days, copper leaching rate is 60.12%, leaches 6 months, and copper leaching rate is 80.75%.
Embodiment 2 (its block diagram such as Fig. 2)
At first to containing thiobacillus ferrooxidant, the acid resistant mutagenic mixed bacterium of thiobacillus thiooxidans and the little spirillum of iron protoxide (deposit in the Wuhan University of China national typical case culture collection center by this bacterium, the culture name is called Thiobacillus ferrooxidans Retech III, preservation registration number is CCTCC No:M200033) be 1.3-1.4 in the pH value, temperature is to add in the ordinary tap water of natural temperature that 2-9K iron-free basic medium and granularity carry out for the flotation concentrate that accounts for certain Containing Sulfur nickel cobalt ore of 95% less than 200 orders that the domestication of ore-leaching bacteria adaptability is cultivated and amplification culture step by step, wherein bacterial load is the 10-20% of culture cumulative volume, the mineral dust amount that adds is for cultivating the 5-10% of bacterium weight, and obtaining bacteria concentration is 10
7-10
9The adaptability of individual/ml is tamed the acid resistant mutagenic ore immersion bacterium, is used for the bacteria heap leaching of ore then.
Certain Containing Sulfur nickel cobalt copper ore floatation concentrate is carried out granulation (pelletizing), the pelletizing diameter is 5-25mm, built heap in maintenance 24-48 hour again, build stack height 4m, building heap finishes the back and 10% inserts the good acid resistant mutagenic mixed bacterium of adaptability domestication amplification culture and begin to drip and drench a leaching by what drip to drench the liquid cumulative volume, dripping the pH value of drenching liquid is 1.0-1.5, and dripping and drenching extraction time is 3-7 days, and dripping pouring speed is 5-20L/ (m
2H), enter leisure then, leisure time is 2-7 days, and spray carries out in turn with leisure; As leach liquor Ni
2+, Co
2+, Cu
2+Ionic concn enters the metal recovery operation after reaching 1.0g/L, and wherein extraction-back extraction-electrodeposition process is adopted in the recovery of copper, output A level cathode copper, and copper extractant is selected Lix984N for use, and thinner is selected sulfonated kerosene for use.The high-purity nickel of extraction-electrodeposition process output after the precipitation removal of impurities and the metal product of cobalt are adopted in the recovery of nickel and cobalt, and the ore deposit dump leaching of raffinate circulated sprinkling goes out.After leaching 3 months, the leaching yield of nickel, cobalt, copper reaches 89%, 86% and 72% respectively.
Advantage of the present invention is: this mutagenesis bacterium can be worked between pH value 0.9～3.0, compare with existing technology, Leaching cycle shortens 1/3～2/3, and leaching rate improves 10～30%, and production cost reduces 10-30%.
Claims (10)
1, a kind of acid resistant mutagenic bacteria, it is characterized in that: it is the acid resistant mutagenic mixed bacterium of thiobacillus ferrooxidant, thiobacillus thiooxidans, the little spirillum of iron protoxide, name is called Thiobacillus ferrooxidans RetechIII, preservation registration number is CCTCC No:M200033, depositary institution China national typical case culture collection center is in the Wuhan University of address.
2, a kind of heap leaching method of acid resistant mutagenic bacteria, it is characterized in that: it comprises:
(1) with the acid resistant mutagenic mixed bacterium of thiobacillus ferrooxidant, thiobacillus thiooxidans, the little spirillum of iron protoxide, this bacterium preserving number CCTCC No:M200033 through domestication and amplification culture, obtains adaptability acid resistant mutagenic mixed bacterium;
(2) ore is pulverized or with concentrate or mine tailing granulation, maintenance, delivered to subsequently and build dump leaching and go out operation;
(3) with the acid resistant mutagenic mixed bacteria liquid of amplification culture in (1) with spray or drip the pouring mode and add on the ore deposit heap that heap builds;
(4) leach the leach liquor that operation comes out and deliver to the metal recovery operation.
3, the heap leaching method of acid resistant mutagenic bacteria according to claim 2, it is characterized in that: the domestication amplification culture condition of described acid resistant mutagenic mixed bacterium is: add in ordinary tap water that 2-9K iron-free basic medium and granularity carry out less than 200 purpose ores that the domestication of ore-leaching bacteria adaptability is cultivated and amplification culture step by step, the inoculum size of acid resistant mutagenic mixed bacterium is that the temperature of 10-20%, growth of culture cumulative volume is between 4-45 ℃, the pH value is between 0.9-3.0, the breeze amount that adds is the 5-10% of culture gross weight, and obtaining bacteria concentration is 10
7-10
9The adaptability domestication acid resistant mutagenic ore immersion bacterium of individual/ml.
4, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: the particle diameter of described powder ore is less than 50mm.
5, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: the described heap of building is concentrate or the mine tailing ore particle through granulation with ore particle, and its diameter is 5-25mm.
6, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: in the described granulating working procedure, granulation limit, limit adds the acid resistant mutagenic bacteria liquid as one of binder constituents.
7, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: build heap after described pelletizing finished product ball warp is crossed maintenance, the maintenance drying is for drying naturally 24-72 hour.
8, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: the described heap of building is built heap limit spray quinoline or is dripped the solution that pouring contains acid resistant mutagenic bacteria for the limit, and the solution spraying amount is the 8-20% of ore weight.
9, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: described spray or drip to drench and to be spray or to drip and drench leisure in several days several days, adopt the duty of having holidays by turns to leach.
10, according to the heap leaching method of claim 2 or 3 described acid resistant mutagenic bacterias, it is characterized in that: described 5-20% inserts spray liquid or drips the amount of drenching in the liquid through the acid resistant mutagenic bacteria of taming, amplification culture is good is a spray liquid or a pouring liquid total amount.
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| CNB021288313A CN1244685C (en) | 2002-08-15 | 2002-08-15 | Acid resistant mutagenic bacteria and its dump leaching process used for ore |
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|---|---|---|---|
| CNB021288313A CN1244685C (en) | 2002-08-15 | 2002-08-15 | Acid resistant mutagenic bacteria and its dump leaching process used for ore |
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| Publication Number | Publication Date |
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| CN1475586A CN1475586A (en) | 2004-02-18 |
| CN1244685C true CN1244685C (en) | 2006-03-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337228A (en) * | 2010-07-21 | 2012-02-01 | 北京有色金属研究总院 | Thermoacidophile and low grade chalcopyrite ore microbe stepwise leaching process |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100368531C (en) * | 2005-12-05 | 2008-02-13 | 东华理工学院 | Acidophilic leptospirillumferrooxidans and its uses |
| CN104745497B (en) * | 2013-12-30 | 2018-03-16 | 北京有色金属研究总院 | A kind of technique for carrying out high sulfur coal mine biological desulphurization using exclusive desulfurization Mixed Microbes |
| CN107326194A (en) * | 2017-06-09 | 2017-11-07 | 广东省资源综合利用研究所 | The recovery method of copper in a kind of copper coating board |
| CN108018250B (en) * | 2018-01-29 | 2021-07-13 | 武汉新禹智水环保科技有限公司 | Acidithiobacillus ferrooxidans and application thereof in environmental treatment |
| CN114196833B (en) * | 2021-12-17 | 2023-03-24 | 中南大学 | Method for promoting leaching of valuable metals in red mud by using sulfur oxidation of acidophilic microorganisms |
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2002
- 2002-08-15 CN CNB021288313A patent/CN1244685C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337228A (en) * | 2010-07-21 | 2012-02-01 | 北京有色金属研究总院 | Thermoacidophile and low grade chalcopyrite ore microbe stepwise leaching process |
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| CN1475586A (en) | 2004-02-18 |
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Effective date of registration: 20190702 Address after: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Patentee after: Research Institute of engineering and Technology Co., Ltd. Address before: 100088, 2, Xinjie street, Beijing Patentee before: General Research Institute for Nonferrous Metals |
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