CN1242070C - Method for preparing point samples for micro array chip of compound - Google Patents
Method for preparing point samples for micro array chip of compound Download PDFInfo
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- CN1242070C CN1242070C CN 200410014687 CN200410014687A CN1242070C CN 1242070 C CN1242070 C CN 1242070C CN 200410014687 CN200410014687 CN 200410014687 CN 200410014687 A CN200410014687 A CN 200410014687A CN 1242070 C CN1242070 C CN 1242070C
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- porous membrane
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Abstract
The present invention discloses a sample application preparation method for a compound microarray chip. First, a block body provided with a hole is adopted; then, the block body is mechanicaly cut in the radical direction of the hole arranged on the block body to obtain a porous membrane; next, the porous membrane is pasted on the glass slide surface modified by a silylating reagent; finally, compounds are selected one by one according to the types and the distribution of compounds arranged on a compound microarray chip to be prepared, and the compounds are dripped into the hole of the porous membrane to take chemical reaction with active groups arranged on the modified glass slide surface, so the compounds are fixed to the carrier surface, and the fixed compounds forming the compound microarray chip. The present invention has the advantages of low preparation cost, simple operation, easy implementation and regular array point arrangement, and is suitable for standardized mass production. The compound microarray chip prepared by the present invention has the advantage that compounds arranged on the array point have the uniform molecular density.
Description
Technical field
The present invention relates to a kind of preparation method of micro-array chip of chemical compound, relate in particular to the some sample preparation method of micro-array chip of chemical compound.
Background technology
Compound microarray spotting template preparation method is that a kind of new UltraDialUp is crossed the new tool that the point sample legal system is equipped with biochip.Biochip mainly refers to the microarray that the biologically active substance (comprising nucleic acid, protein, cell and small tissue etc.) assembled constitutes in the solid chip, with realize to compound (comprising medicine), protein, nucleic acid, cell and other biological components accurately, fast, the screening or the detection of large information capacity.Porous check-out console (as 96 orifice plates) is the existing a kind of conventional utensil that is used for medicine, biochemistry detection and screening that generally uses.Respectively different proteins, nucleic acid probe, cell strain or biological tissue are placed or be fixed among the different holes, by adding different chemicals, or compound combination, or biochemical reagents, or detected biological sample, observe the reaction of different chemical/biological substance, carry out fast parallel biochemical analysis, Clinical Laboratory or drug screening.96 reaction tanks on it are isolated from each other, and can select the number of reaction tank arbitrarily for use according to user's needs.This is existing a kind of method that is used for Biochemical Research and exploitation of generally using.But this method still needs one by one different compounds, biomolecules etc. are assigned among each hole, preparation process is still complicated, especially the reagent cost height also is not easy to operate continuously fast simultaneously, more is not easy to simultaneously to multi-component continuous rapid detection in the sample.It is earlier the biomolecules of analyzing to be fixed on substrate material to connect that the point sample legal system is equipped with biochip.Its main drawback is that probe density is not high.But new way and means as a kind of biological collimation analysis have flexibly, simple, characteristics such as investment is not high, thereby are a kind of means and methods that various biological samples carry out scientific research in early days that be particularly suitable for.But the expense of putting foreign instrument at present is unfavorable for promoting up to hundreds of thousands of Renminbi; Though artificial point sample then has cheap outstanding of expense, also exists the sample lattice point misaligned, the sample lattice point irregular with the sample lattice point on inhomogeneous with the consistent shortcoming of bonded compound molecule quantity.
Technology contents
The present invention proposes that a kind of preparation cost is low, operation is simple, lattice point marshalling and be applicable to the some sample preparation method of the micro-array chip of chemical compound that stdn is produced in batches, adopts on the chemical array chip lattice point that The compounds of this invention micro-array chip point template makes compound molecule density even.
The present invention adopts following technical scheme, that is: get earlier and establish the foraminous block, radially block is carried out cut mechanically along the hole on the block then, obtain porous membrane, again porous membrane is fitted on the glass slide surface of modifying by silylating reagent, at last, according to classes of compounds on the micro-array chip of chemical compound to be prepared and distribution thereof, choose compound one by one and it is splashed in the hole of porous membrane and with modified glass slide lip-deep active group generation chemical reaction and make it be fixed on carrier surface, the formationization micro-array chip of chemical compound with the point sample method.
Compared with prior art, the present invention has following advantage:
1) because block is carried out cut mechanically, can obtain many identical porous membranes simultaneously, then obtain many identical porous membranes that are fitted in the glass slide surface, according to the needs of classes of compounds on the micro-array chip of chemical compound to be prepared and distribution thereof, can prepare many identical or different compound chips.Thereby, can stdn and produce quantitatively, preparation cost is low;
2) the present invention had both had the equipment advantage simple, with low cost that manual point sample preparation method is had, and can make the compound molecule lattice point marshalling that obtains again, was convenient to scanning analysis;
3) it splashes in the hole of porous membrane with compound with the point sample method, and compound solution can effectively be retained in its diaphragm hole that splashes into, and can not move around the hole, guarantees that compound solution fully contacts concurrent biochemical reaction with the glass slide surface.Therefore compound molecule density is even on the lattice point that obtains, and makes the accuracy height of chemical array chip analysis;
4) the point sample instrument point sample prepares the chemical array chip and compares, and does not need expensive point sample equipment, and the making ability of chemical array chip is greatly enhanced simultaneously.
Specific embodiment
A kind of some sample preparation method of micro-array chip of chemical compound, get earlier and establish the foraminous block, radially block is carried out cut mechanically along the hole on the block then, obtain porous membrane, again porous membrane is fitted on the glass slide of modifying by silylating reagent, at last, according to classes of compounds on the micro-array chip of chemical compound to be prepared and distribution thereof, choosing compound one by one also splashes into it in hole of porous membrane with the point sample method, and with the lip-deep active group of modified glass slide (as amino, aldehyde radical etc.) chemical reaction taking place and make it be fixed on the surface, makes these fixed compounds form micro-array chip of chemical compound.This compound molecule can be the polymer compound molecule, for example: materials such as oligonucleotide, polypeptide, polysaccharide, peptide nucleic acid(PNA), protein and other and cell thereof, above-mentioned porose block can take following two kinds of methods to prepare: (1) gets block materials, obtaining porose block after the boring on the block materials, this block materials can be an organic polymer material, as: rubber, plastics, paraffin, gel, nylon, biomacromolecule or poly-electric polymer etc. also can be organic polymer composite; (2) straight tube is arranged in parallel, filling block materials between straight tube makes it form porose block again, in the present embodiment, hole on the block in having finished the hole that compound is splashed into porous membrane and after the operation that is fixed, is taken off the porous membrane that is fitted on the slide glass by array distribution.The slide glass surface promptly constitutes the chemical array chip by these compounds.
Embodiment one: the preparation of oligonucleotide arrays film chip
The hollow fiber conduit that some intensive that be arranged in parallel, internal diameter 0.35 mm outer diameter are 0.40 millimeter is sticking and become an integral body by tackiness agent, isolates mutually by tackiness agent between tubular fibre; Obtain comprising the diaphragm of a series of hollow fiber conduits by the machinery section along the vertical direction of hollow fiber conduit; With the diaphragm subsides with on the aldehyde group modified substrate of a process; Splash in the different holes of porous membrane with the oligonucleotide solution of point sample method with end modified amino, the lip-deep aldehyde groups chemical reaction of amino on the oligonucleotide and glass slide and oligonucleotide is fixed takes off the porous membrane that is fitted on the slide glass.The slide glass surface promptly constitutes the oligonucleotide arrays chip by these oligonucleotide.
Embodiment two: the preparation of albuminous membranae chip
On the block olefin material, get out some intensive parallel straight line ducts, isolate by olefin material between the straight line duct; Obtain a series of diaphragms that comprise the straight line duct along the vertical direction in straight line duct by the machinery section; Have on the glass substrate of aldehyde groups through modification, surface with the diaphragm subsides with in one; Protein molecule solution is splashed in the different holes of porous membrane with the point sample method, the lip-deep aldehyde groups chemical reaction of amino on the protein molecule and glass slide and protein molecule is fixed takes off the porous membrane that is fitted on the slide glass.The slide glass surface promptly constitutes the protein array chip by these protein molecules.
Claims (3)
1, a kind of some sample preparation method of micro-array chip of chemical compound, it is characterized in that getting earlier and establish the foraminous block, radially block is carried out cut mechanically along the hole on the block then, obtain porous membrane, again porous membrane is fitted on the glass slide surface of modifying by silylating reagent, at last, according to classes of compounds on the micro-array chip of chemical compound to be prepared and distribution thereof, choose compound one by one and it is splashed in the hole of porous membrane and with modified glass slide lip-deep active group generation chemical reaction and make it be fixed on carrier surface, the formationization micro-array chip of chemical compound with the point sample method.
2, the some sample preparation method of micro-array chip of chemical compound according to claim 1 is characterized in that getting block materials, is obtaining porose block after the boring on the block materials.
3, the some sample preparation method of micro-array chip of chemical compound according to claim 2 is characterized in that hole on the block is by array distribution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410014687 CN1242070C (en) | 2004-04-19 | 2004-04-19 | Method for preparing point samples for micro array chip of compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410014687 CN1242070C (en) | 2004-04-19 | 2004-04-19 | Method for preparing point samples for micro array chip of compound |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1563416A CN1563416A (en) | 2005-01-12 |
| CN1242070C true CN1242070C (en) | 2006-02-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410014687 Expired - Fee Related CN1242070C (en) | 2004-04-19 | 2004-04-19 | Method for preparing point samples for micro array chip of compound |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1242070C (en) |
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2004
- 2004-04-19 CN CN 200410014687 patent/CN1242070C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1563416A (en) | 2005-01-12 |
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Granted publication date: 20060215 |