CN1238382A - Application of chrysobacillus cereus as strain in synthesis of polyhydroxyl butyrate - Google Patents
Application of chrysobacillus cereus as strain in synthesis of polyhydroxyl butyrate Download PDFInfo
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- CN1238382A CN1238382A CN99109820A CN99109820A CN1238382A CN 1238382 A CN1238382 A CN 1238382A CN 99109820 A CN99109820 A CN 99109820A CN 99109820 A CN99109820 A CN 99109820A CN 1238382 A CN1238382 A CN 1238382A
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- phb
- golden yellow
- molasses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Abstract
the present invention relates to the use of chrysobacillus cereus as the strain to synthesize polyhydroxyl butyrate (PHB) that is a degradable biologic material, belonging to industrial fermentation engineering with microbes. The culture medium as growing carbon source is chosen from molasses, cane sugar, glucose, or starch. The said culture medium as carbon source is used for synthesizing polyhydroxyl butyrate. Its advantages include quick growth in waste molasses, no need of modifying existing fermenter equipment, and low cost of production.
Description
The invention belongs to the industrial microorganism field of fermentation engineering.
Poly butyric ester (Poly-β-hydroxybutyrate is called for short PHB) is by a kind of biological polyester of bacterium synthetic, is particulate state with the inclusion form and exists in the bacterium born of the same parents.The one-tenth plasticity that it possesses, mechanical property is similar to the present oil plastics of widely applying, and PHB has biodegradability.So the PHB that produces with the method for microbial fermentation is considered to a kind of " biodegradable plastic " and extremely attention.The bacterial classification of existing production poly butyric ester, as the true bacteria Ralstonia of Luo Shi eutropha, huge Alcaligenes Alcaligenes latus, vickers nitrogen-fixing bacteria Azotobactervinelandii etc., the industrial production that can both be used for PHB, but its production rate is also unsatisfactory.
The objective of the invention is to for overcoming the weak point of prior art, search out the new PHB bacterial strain of a strain one golden yellow bacillus aureus, under similar condition, obtain the speed of growth faster than above-mentioned bacterial classification, and can be further by selecting suitable culture condition, obtain the poly butyric ester PHB of high yield, thereby obtain biodegradable plastic cheaply.
The present invention proposes with the bacterial classification of the golden yellow genus bacillus of a strain as synthesized degradable biomaterial poly butyric ester.
The present invention also proposes to produce with golden yellow genus bacillus the method for poly butyric ester, it is characterized in that, used substratum comprises any do growth carbon source of using earlier in molasses, sucrose, glucose or the starch, and then makes the synthetic carbon source substratum of poly butyric ester with molasses, sucrose, glucose or starch.
The present invention can obtain the poly butyric ester of high yield by selecting suitable culture condition, and suitable culture condition comprises the substrate of culture temperature, substratum and concentration thereof, oxygen-supply quantity, incubation time, pH value and synthetic PHB etc.There is bigger difference in the golden yellow genus bacillus of this strain in different growth period forms, and the culture temperature of this method can be in 28～42 ℃ of scopes.The used pH value of this substratum can be in 6.0～8.0 scopes.
When being substratum with molasses, suitable molasses concentration can reach 220g/L; At strain growth oxygen-supply quantity in period, big, the pH value is generally between 6.5～7.5.The general cultivation in fermentor tank can obtain a large amount of bacterial strains early stage, can enter a large amount of accumulation stages of PHB afterwards.It is the substrate raised growth that general elder generation utilizes cheap waste molasses by golden yellow genus bacillus, utilizes the technology of sucrose for a large amount of synthesis of polyhydroxyl butyrate of substrate again, can improve the yield of PHB greatly.
Advantage of the present invention and positively effect
Golden yellow genus bacillus of the present invention can be to grow fast and synthetic PHB in the single nutrition with molasses, can reach 26g/L 8 hours inner cell dry weights, PHB content accounts for 19% of dry cell weight: golden yellow genus bacillus can be in the mineral substratum that with sucrose is carbon source high yield PHB, the content of PHB accounts for the 70-80% of dry cell weight: golden yellow genus bacillus utilizes cheap waste molasses to be the substrate raised growth earlier, utilize sucrose to be a large amount of synthesis of polyhydroxyl butyrate of substrate again, reach 76g/L in 50 hour cell dry weights, PHB content 75%.
Utilize golden yellow genus bacillus, adopt suitable fermentation process, can in waste molasses, form biomass fast, and produce PHB.In sucrose, this bacterium can efficiently be synthesized PHB to the 70-80% that accounts for dry cell weight.Under the situation that does not change fermentation equipment, can reduce the production cost of poly butyric ester PHB.On the other hand, golden yellow genus bacillus can utilize cheap waste molasses to be the substrate raised growth earlier, utilizes sucrose to be a large amount of PHB of substrate again, can improve the yield of PHB so greatly.Reduce the production cost of biodegradable plastic PHB.
The present invention proposes four kinds of embodiment that produce the method for poly butyric ester with golden yellow genus bacillus
Embodiment 1: the growth of golden yellow genus bacillus in molasses and the accumulation of PHB
Bacterial classification: golden yellow genus bacillus:
Culture temperature: 30 ℃;
Substratum: molasses (as unique substratum)
Substratum concentration: 180g/L;
Air flow: the 4-7 liter/minute, the air flow when beginning to ferment is 4 liters/minute, slowly increases with the increase of bacterial density later on)
Fermentation time: 40 hours;
The technological process of production of present embodiment is as follows:
Golden yellow genus bacillus is at the fermentor tank of 5L, under 30 ℃, blowing air fermentation culture 40 hours in the molasses of 180g/L (air flow be the 4-7 liter/minute, air flow when beginning to ferment is 4 liters/minute, after slowly increase), after 12,16,20 hours, mend 400,300 respectively, 300ml contains the substratum that molasses concentration is 500g/L.Centrifugal to institute's sample thief after fermentation ends, washing, analyze the data obtain dry cell weight and PHB content.
The dry cell weight maximum value that obtains after 40 hours of fermenting is 50g/L, and it is 17% that corresponding with it PHB accounts for the dry cell weight percentage composition.
Embodiment 2: air flow is to the influence of the growth of golden yellow genus bacillus in molasses
Bacterial classification: golden yellow genus bacillus;
Culture temperature: 36 ℃;
Substratum: molasses
Substratum concentration: 180g/L;
Air flow: keep 7 liters/minute
Fermentation time: 8 hours;
The technological process of production of present embodiment is as follows:
Golden yellow genus bacillus is at the fermentor tank of 5L, under 36 ℃, and 8 hours (air flow keeps 7 liters/minute) of blowing air fermentation culture in the molasses of 180g/L.Centrifugal to institute's sample thief after fermentation ends, washing, analyze the data obtain dry cell weight and PHB content.
Dry cell weight reaches 26g/L after 8 hours, and corresponding with it PHB percentage composition is 19%.
Embodiment 3: the growth of golden yellow genus bacillus in sucrose and the accumulation of PHB
Bacterial classification: golden yellow genus bacillus;
Culture temperature: 32 ℃;
Substratum: sucrose 90g/L (stream adds);
Substratum sucrose initial concentration: 2g/L;
Initial phosphorus content: 1.8g/L;
Initial nitrogen content: 1.3g/L;
Fermentation time: 50 hours;
The technological process of production of present embodiment is as follows:
Golden yellow genus bacillus is at the fermentor tank of 5L, and under 32 ℃, the sucrose concentration that adds at stream is about among the 2g/L 50 hours (air flow keeps 5 liters/minute) of fermentation culture.Centrifugal to institute's sample thief after fermentation ends, washing, analyze the data obtain dry cell weight and PHB content.
Dry cell weight reaches 31g/L after 50 hours, and corresponding with it PHB percentage composition is 78%.
Embodiment 4: growth and in sucrose the accumulation of PHB of golden yellow genus bacillus in molasses
Bacterial classification: golden yellow genus bacillus;
Culture temperature: 37 ℃;
Substratum: molasses (220g/L), sucrose (90g/L, stream adds);
Air flow: keep 7 liters/minute
Fermentation time: 50 hours;
The technological process of production of present embodiment is as follows:
Golden yellow genus bacillus is at the fermentor tank of 5L, and under 37 ℃, growth is after 20 hours in the molasses of 220g/L, and Continuous Flow added sucrose 20 hours, fermentation culture 50 hours.Centrifugal to institute's sample thief after fermentation ends, washing, analyze the data obtain dry cell weight and PHB content.
Dry cell weight reaches 76g/L after 50 hours, and corresponding with it PHB percentage composition is 75%.
Claims (4)
1, with the bacterial classification of the golden yellow genus bacillus of a strain as synthesis of polyhydroxyl butyrate.
2, the method for the golden yellow genus bacillus production of a kind of usefulness poly butyric ester, it is characterized in that, used substratum comprises any do growth carbon source of using earlier in molasses, sucrose, glucose or the starch, and then makes the synthetic carbon source substratum of poly butyric ester with molasses, sucrose, glucose or starch.
3, the method for the golden yellow genus bacillus production of usefulness as claimed in claim 2 poly butyric ester is characterized in that culture temperature is in 28～42 ℃ of scopes.
4, the method for the golden yellow genus bacillus production of usefulness as claimed in claim 2 poly butyric ester is characterized in that the used pH value of substratum is in 6.0～8.0 scopes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99109820A CN1238382A (en) | 1999-07-16 | 1999-07-16 | Application of chrysobacillus cereus as strain in synthesis of polyhydroxyl butyrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99109820A CN1238382A (en) | 1999-07-16 | 1999-07-16 | Application of chrysobacillus cereus as strain in synthesis of polyhydroxyl butyrate |
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| Publication Number | Publication Date |
|---|---|
| CN1238382A true CN1238382A (en) | 1999-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN99109820A Withdrawn CN1238382A (en) | 1999-07-16 | 1999-07-16 | Application of chrysobacillus cereus as strain in synthesis of polyhydroxyl butyrate |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421714A (en) * | 2013-08-02 | 2013-12-04 | 华南理工大学 | Bacillus shackletonii and application thereof in fermentation production of polyhydroxybutyrate |
| CN107857844A (en) * | 2017-11-15 | 2018-03-30 | 成都测迪森生物科技有限公司 | A kind of preparation method of the poly- β hydroxybutyric acids of graft modification |
| CN107879392A (en) * | 2017-11-29 | 2018-04-06 | 成都创客之家科技有限公司 | A kind of Drinking Water Filtration core |
| CN111394398A (en) * | 2020-06-03 | 2020-07-10 | 中粮营养健康研究院有限公司 | Method for preparing PHA (polyhydroxyalkanoate) by fermenting high-salt molasses serving as raw material |
-
1999
- 1999-07-16 CN CN99109820A patent/CN1238382A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421714A (en) * | 2013-08-02 | 2013-12-04 | 华南理工大学 | Bacillus shackletonii and application thereof in fermentation production of polyhydroxybutyrate |
| CN103421714B (en) * | 2013-08-02 | 2014-12-31 | 华南理工大学 | Bacillus shackletonii and application thereof in fermentation production of polyhydroxybutyrate |
| CN107857844A (en) * | 2017-11-15 | 2018-03-30 | 成都测迪森生物科技有限公司 | A kind of preparation method of the poly- β hydroxybutyric acids of graft modification |
| CN107879392A (en) * | 2017-11-29 | 2018-04-06 | 成都创客之家科技有限公司 | A kind of Drinking Water Filtration core |
| CN111394398A (en) * | 2020-06-03 | 2020-07-10 | 中粮营养健康研究院有限公司 | Method for preparing PHA (polyhydroxyalkanoate) by fermenting high-salt molasses serving as raw material |
| CN111394398B (en) * | 2020-06-03 | 2020-09-01 | 中粮营养健康研究院有限公司 | Method for preparing PHA (polyhydroxyalkanoate) by fermenting high-salt molasses serving as raw material |
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