CN1232873A

CN1232873A - Production of spore lactic acid bacteria powder

Info

Publication number: CN1232873A
Application number: CN 98101400
Authority: CN
Inventors: 杜连祥; 戚薇; 王艳萍; 陈莹
Original assignee: TIANJIN LIGHT INDUSTRY COLLEGE
Current assignee: TIANJIN LIGHT INDUSTRY COLLEGE
Priority date: 1998-04-20
Filing date: 1998-04-20
Publication date: 1999-10-27
Anticipated expiration: 2018-04-20
Also published as: CN1117144C

Abstract

Spore lactic acid bacteria strain is processed through the steps of slant culture in test tube containing bran water, shaking culture in shaking bottle containing seed culture medium, seed tank culture while aeration and stirring, fermentation pot culture in pot with fermenting culture medium, addition of maltodextrin, stirring homogeneously and spraying to dry to produce spore lactic acid bacteria powder with high live bacteria content. The said process can produce spore lactic acid bacteria product with strong acid resistance, long preservation period and wide application.

Description

The production method of spore lactic acid bacteria powder

The present invention relates to a kind of production method of lactic acid bacteria powder, particularly relate to a kind of production method of spore lactic acid bacteria powder.

Currently just ordinary lactic acid bacteria is made pulvis,,, cause energy consumption height, apparatus expensive, cost higher because of vacuumizing and freezing so the production of its powderous preparations only limits to vacuum freeze-drying method because ordinary lactic acid bacteria exists with heat labile nourishing body cell; And the cell survival rate in the lactic acid bacteria powder of making is low, thermotolerance and acid resistance are poor, and preservation period is short, range of application is narrow.

The objective of the invention is to provide a kind of and produce lactic acid bacteria viable bacteria pulvis with aerated culture and spray-drying process in order to overcome above-mentioned deficiency.

The present invention is achieved in that this method in turn includes the following steps:

1. the test tube slant is cultivated: with a strain lactic acid bacteria in the test tube slant that contains the bran water composition

On, temperature is 37-47 ℃ and carries out activation culture 2-3 time;

2. shake-flask culture: thalline inserted this seed culture is housed in the triangular flask of seed culture medium

Base is to be main charcoal source and to add acid neutralizing agent CaCO with glucose ₃Liquid culture

Base, temperature are that the 37-47 ℃ of rotating speed shaking table with 140-180 rev/min cultivated 18-24 hour;

3. seed tank culture: the inoculum size with 5-10% is connected in the seeding tank that seed culture medium is housed,

At air flow is that 1:0.5-0.7 (V.V.m), mixing speed are 300-400 rev/min, temperature

Spend under 37-47 ℃ the condition and cultivated 16-24 hour;

4. fermentor cultivation: insert the stirring-type that fermention medium is housed with the inoculum size of 5-10% and send out

In the ferment jar, fermention medium is a kind of Mn that contains that gemma is formed with promoter action ²⁺Liquid

The body substratum, fermentation condition is: temperature 40-50 ℃, air flow 1:0.5-0.7 (V.V.m),

Mixing speed is 400-500 rev/min, incubation time 65-75 hour;

The optimum temps of above step is 40-43 ℃.

5. spraying drying: put into the fermented liquid and the maltodextrin (DE15-20) of fermentation liquid measure 20% mixed

Close and carry out spraying drying after mixing in the jar;

It is 10 that the powderous preparations that makes by above step contains heat-resisting lactic acid bacteria bulk concentration ⁹Individual/gram.

The culture temperature of thalline is 40-45 ℃, and shaking bottle rotating speed 140-180 rev/min, fermentor tank air flow 1:0.5-0.7 (V.V.m), mixing speed is 400-500 rev/min.The preparation of pulvis adopts spray-dired method to obtain.

Advantage of the present invention is: because lactic acid bacteria can form the strong gemma of thermotolerance.The production of its pulvis can be adopted aerated culture and spray-drying process, this method technology is simple, equipment is cheap, energy consumption is little, cost is low, makes pulvis with this method through cultivating the formation gemma, has advantages such as thermotolerance and acid resistance are strong, long preservative period, applied range.

Embodiment 1: shake-flask culture (laboratory scale)

One strain lactic acid bacteria is inoculated on the test tube slant of the solid slant culture base that contains the bran water composition, temperature is 40 ± 1 ℃ and cultivated 20 hours, wash thalline with the 5-6ml sterilized water, the 1ml bacteria suspension is inserted in the triangular flask of the 1000ml capacity that 100ml seed liquid nutrient medium is housed, then 40 ℃ of temperature.Rotating speed is to cultivate 20 hours on 150 rev/mins the shaking table, again according to 10% inoculum size, seed culture fluid is inserted in the triangular flask of the 3000ml capacity that the 500ml fermentation broth is housed, 40 ℃ of temperature, shake bottle rotating speed and be 160 rev/mins, cultivated 72 hours, the maltodextrin (DE20) that adds fermentation liquid measure 20% then stirs in the cultured fermented liquid and carries out can obtaining containing lactic acid bacteria viable bacteria pulvis after the spraying drying, and its viable bacteria bulk concentration is 1-2 * 10 ⁹Individual/gram dry powder.

Embodiment 2: fermentor cultivation (productivity scale)

Slant culture and shake-flask culture are undertaken by embodiment 1 method, and its back operation is carried out according to the following steps.

1. first order seed is cultivated: the inoculum size with 10% inserts in the triangular flask of the 3000ml that the 500ml seed culture medium is housed, and 42 ℃ of temperature were cultivated 24 hours;

2. secondary seed is cultivated: the inoculum size with 5% is inoculated in the 200L fermentor tank that the 100L seed culture medium is housed, and 42 ℃, air flow is 1:0.7 (V.V.m), and mixing speed is 400 rev/mins, incubation time 24 hours;

3. fermentor cultivation: the inoculum size with 10% inserts and is equipped with in the 1500L fermentor tank of 1000L fermention medium, and 42 ℃ of temperature, air flow 1: 0.7 (V.V.m), mixing speed are 400 rev/mins, cultivated 60 hours;

4. spraying drying: fermented liquid is carried out spraying drying after the maltodextrin of DE value 15 (consumption be fermented liquid 20%) mixes in mixing tank; Obtaining containing lactic acid bacteria viable bacteria viable bacteria bulk concentration is 1-2 * 10 ⁹Individual/as to restrain viable bacteria pulvis in powder.

Claims

1. the production method of a spore lactic acid bacteria powder, it is characterized in that adopting aerated culture and spray-drying process, and in turn include the following steps: 1. the test tube slant is cultivated: on the test tube slant that contains the bran water composition, temperature is 37-47 ℃ and carries out activation culture 2-3 time with a strain lactic acid bacteria; 2. shake-flask culture: thalline inserted be equipped with in the triangular flask of seed culture medium, this seed culture medium is to be main coal source and to add acid neutralizing agent CaCO with glucose ₃Liquid nutrient medium, temperature is 37-47 ℃ and cultivated 18-24 hour with 140-180 rev/min rotating speed shaking table; 3. seed tank culture: the inoculum size with 5-10% is connected in the seeding tank that seed culture medium is housed, and is that 1:0.5-0.7 (V.V.m), mixing speed are to cultivate 16-24 hour under the condition of 300-400 rev/min, temperature 37-47 ℃ at air flow; 4. fermentor cultivation: insert with the inoculum size of 5-10% and to be equipped with in the stirring-type fermentor tank of fermention medium, fermention medium is a kind of Mn that contains that gemma is formed with promoter action ²⁺Liquid nutrient medium, fermentation condition is: temperature 40-50 ℃, air flow 1:0.5-0.7 (V.V.m), mixing speed are 400-500 rev/min, incubation time 65-75 hour, and optimum temps is 40-43 ℃; 5. spraying drying: carry out spraying drying after the fermented liquid and the maltodextrin (DE15-20) of fermentation liquid measure 20% put into mixing tank and mix;

Can make the powderous preparations that contains heat-resisting lactic acid bacteria body by above step;

2. method according to claim 1 is characterized in that: the culture temperature of thalline is 40-45 ℃, and shaking bottle rotating speed 140-180 rev/min, fermentor tank air flow 1:0.5-0.7 (V.V.m), mixing speed is 400-500 rev/min.The preparation of pulvis adopts spray-dired method to obtain.