CN1226843A - Enzymatic method for removing contaminants from ion exchange and fractionation resin - Google Patents
Enzymatic method for removing contaminants from ion exchange and fractionation resin Download PDFInfo
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- CN1226843A CN1226843A CN97196893A CN97196893A CN1226843A CN 1226843 A CN1226843 A CN 1226843A CN 97196893 A CN97196893 A CN 97196893A CN 97196893 A CN97196893 A CN 97196893A CN 1226843 A CN1226843 A CN 1226843A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J49/00—Regeneration or reactivation of ion-exchangers; Apparatus therefor
- B01J49/50—Regeneration or reactivation of ion-exchangers; Apparatus therefor characterised by the regeneration reagents
- B01J49/57—Regeneration or reactivation of ion-exchangers; Apparatus therefor characterised by the regeneration reagents for anionic exchangers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J49/00—Regeneration or reactivation of ion-exchangers; Apparatus therefor
- B01J49/50—Regeneration or reactivation of ion-exchangers; Apparatus therefor characterised by the regeneration reagents
- B01J49/53—Regeneration or reactivation of ion-exchangers; Apparatus therefor characterised by the regeneration reagents for cationic exchangers
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Abstract
The present invention relates to an enzymatic method used to clean resins, particularly ion exchange and fractionation resins. The method can be used alone or in combination with chemical methods. The method allows for improved production of liquids that contain contaminants as for example proteins, carbohydrates, lipids, and residual unconverted starches that require fractionation or ion exchange treatment. The invention also relates to the use of resins for the production of corn sweeteners, in particular, corn syrup and high fructose corn syrup.
Description
Background of invention
Invention field
The present invention relates to the broad field of enzyme processing technology.More particularly, the present invention relates to a kind of by from resin especially ion-exchange and fractionation resin, remove the method for pollutant with the enzyme facture.The invention still further relates to a kind of method of utilizing the prior resin that cleaned with enzyme to produce cereal sweetener, especially the cereal syrup and the HFCS of purifying.
Description of related art
In analytical chemistry, resin provides strong instrument for separating organic or inorganic ion or nonionic sample.Resin normally is included in pillar or the pipeline.The material of process of resin self-crosslinking polystyrene and so on, they are also claimed DVB (divinylbenzene) by chemically crosslinked together, and the form of macropore (macroscopic void) and gel (aperture) is arranged.Other used in food processing resin is from esters of acrylic acid.By this resin of regenerating, resin is recycling usually.Contaminated thing causes and deactivates or pollution can destroy resin.Except that thermal instability (desulfonation or forfeiture functionalization), ionic, graininess deposit and polymerism material also can cause pollution.
Ion exchange resin is a kind of synthetic organic polymer, and it usually is based on crosslinked polystyrene, will exchange all materials of counter ion counterionsl gegenions by adding when the charged groups generation is suspended in the aqueous solution, thereby derive this material.Cationic ion-exchange resin has fixing acid substituting group, and this acidity substituting group is based on material such as strong acid exchanger sulfonic acid or weak acid exchanger carboxylic acid.That anion exchange resin has is fixing, based on substituting group as alkalescent exchangers such as quaternary ammonium or ethoxy amido or ammonium classes.Other functional group can be attached on the resin matrix so that it has more the selection effect.The derivatization degree of resin and the degree of cross linking have determined the total capacity of ion-exchange.
A kind of technology that is called chromatography be based on each material between liquid phase and solid phase distribution capability and be used for separating the mixture of various materials.Ion-exchange chromatography is defined as such class chromatography, and solid support wherein is a kind of ion exchange material that is used for the mixture of separating charged molecule or ion.This can carry out on the preparation scale level, or as a kind of change form of high pressure liquid chromatography (HPLC).
Chromatography is decided by to exchange the different of potentiality, eluant, eluent, column length and application of sample amount, flow velocity, granular size and temperature.Chromatography can be used to comprise the cereal sweetener in the wide purposes, as the cereal syrup (eoru syrup, CSU) and HFCS (high fructose cornsyrup, purifying HFCS).Can make the cereal sweetener by hydrolysed corn starch.The composition that has made is provided a glucose equivalent, and (it is decided by to react the transforming degree that is allowed for dextrose equivalent, D.E.) number.This numerical value is low more, and the degree of conversion is also low more.High more D.E. numeric representation transforms more completely, and 92D.E. represents to have changed into glucose (cerealose) from cereal starch fully.HFCS can be with enzymatic conversion to 97D.E., and is classified into 97-99D.E..The cereal sweetener has been used to fine jade pouring of preparation ice and yogurt fruit for many years.It is topmost in a series of reasons that need the cereal sweetener to mix two reasons being arranged.At first, the cereal sweetener gives the product made from desired shapes or " mouthfeel ", and this is single inaccessiable with sucrose institute.Secondly, every pound of some cereal sweetener can mix higher sugariness than sucrose, thereby uses them more cheap.Obviously, because the important value of cereal sweetener, the improving one's methods of resin that can be used for preparing the cereal sweetener of purifying that be used for regenerating will be very useful.At present, selectable method when ion-exchange separates chromatography and is purifying cereal sweetener with classification, however the post or the pipeline of dress resin are contaminated through regular meeting, and be difficult to clean.
The method of selecting at present of regenerating resin of being used for comprises the use of chemicals.Normally used chemicals comprises following but is not limited to these: hydrochloric acid (HCl), NaOH (NaOH), ammonium hydroxide (NH
4OH) and sodium carbonate (Na
2CO
3).At first, with irrigation (water commonly used) flushing resin, carry out a series of chemical treatment steps then.Yet, even sometimes through chemical treatment or regeneration after, resin is still polluted by microorganism (as bacterium), this situation is much.
One object of the present invention is exactly in a unemployed manufacture field, to provide a kind of particularly method that is used for cleaning resin of protease, lipase, carbohydrase and AMS of enzyme of utilizing at enzyme.The present invention is particularly suitable for cleaning ion-exchange and the fractionation resin that is used for purifying cereal sweetener.
Summary of the invention
The present invention relates to a kind of enzymatic method that is used to clean resin, especially ion-exchange and fractionation resin.This method can be used separately or unite use with chemical method.For the liquid that has comprised the pollutant that needs classification separation or ion-exchange treatment such as protein, carbohydrate, lipid and residual unconverted starch etc., this method can improve their output.This invention also relates at the cereal sweetener, especially the purposes of resin in the production of cereal syrup and HFCS.
The description of all preferred embodiments
The present invention relates to from resin especially ion-exchange and fractionation resin, remove a kind of enzymatic method of pollutant.All enzymes are exposed in the resin that has polluted, and optionally in the special time of appointment, make enzyme recirculation passing through resin.The time length of enzyme recirculation is production factors.The time that is used for process resin is short more, and it is just big more to clean the required enzyme dosage of resin.
The present invention can join together the new purposes and the conventional chemical treatment techniques of enzyme, thereby removes pollutant from resin.Enzyme has the optimal pH of a feature, and their activity is the highest when this pH value.The pH-activity curve of enzyme has reflected the pH value when proton donor important in the enzymatic site or proton acceptor group are in desired ionization state.The optimal pH of enzyme needn't be consistent with the pH value of home, and environment pH value can be lower than optimal pH high slightly or slightly.
The present invention can with can be at pH1-9, more preferably between the pH6-8, especially more preferably pH6.5-7.5 and optimum temperature 90-180F, more preferably all existing protease, lipase, carbohydrase and the AMS that acts under the 120-130F condition uses together.
Can be used among the present invention so long as those skilled in the art will understand plurality of enzymes, depend on the pollutant that throws into question with any enzyme.Following enzyme is conventional the use, and existing only providing as an example do not wish to have limited scope of the present invention.It in the bracket behind the common name of following enzyme their trade name.
Protease/peptase (Alcalase, Neutrase, Flavourzyme)
1,4 beta-glucanase (Finizym, Ceveflo)
Lipase (Palatase, Lipozyme)
AMS (BAN, Termamyl L type)
Carbohydrase (Viscozyme)
Multienzyme complex comprises following kind but is not limited to this:
AMS, 1,4 beta-glucanase, protease (Ceremix), carbohydrase
(Viscozyme)
For all purposes of the application, protease/peptase is defined as hydrolyzable (fracture) protein and becomes short chain, soluble peptide and amino acid whose class of enzymes.
1,4 beta-glucanase is defined as the class of enzymes that hydrolyzable beta glucan (1,4-beta glucan, 1,3-beta glucan) becomes oligosaccharides and disaccharide.1,4-β and 1, the sign of 3-β is meant the chemical bond between the carbon atom that this fermentoid acted in the molecule.
Oligosaccharides is defined as the short chain of the dextran molecule that chemistry links together.The length of these chains is 3-5 glucose unit normally.
Two glucose units that disaccharide is defined as linking together.This is in the only a small amount of on formation of 1,4 beta-glucanase.
Lipase is defined as can be 1, and the 3-esterification connects the class of enzymes that the site is hydrolyzed into short chain and LCFA in monoglyceride or glycerine dibasic acid esters and aliphatic acid.SCT refers to have the triglycerides of the side chain that is no more than 12 carbon molecules, and promptly the ester of dodecylic acid and carbon number are than its little ester.LCFA refers to have the aliphatic acid of 12 above carbon molecules, i.e. the above ester of dodecylic acid.
But AMS is defined as α-1,4 glycosidic bond in random hydrolysis amylose and the amylopectin, causes forming the class of enzymes of soluble dextrins and oligosaccharides.The chemistry that α-1,4 glycosidic bond refers to constitute between all glucose molecules of starch connects.No. 1 carbon atom bonding of a glucose molecule is to No. 4 carbon atom of adjacent molecule.This key is attacked by AMS just, produces the catabolite-dextrin and the oligosaccharides of starch.
The starch that two kinds of fundamental types are arranged: amylose and amylopectin.Amylose strictly is made of α-1,4 glycosidic bond, can be decomposed by AMS.Amylopectin is second kind of starch, and it is by α-1, and 4-glycosidic bond and α-1,6 glycosidic bond constitutes.AMS can not cut off 1,6 key, so amylopectin is difficult by α-Dian Fenmeishuixie.
Carbohydrase is defined as class scope enzyme wider, the non-starch based polysaccharide of hydrolyzable (β-1,4-α-1,4-α-1,5).This fermentoid comprises: arabanase, cellulase, 1,4 beta-glucanase, hemicellulase and zytase.Arabanase is hydrolyzed into few araban and arabinose (arabanose) with araban.Cellulase becomes few cellulose and cellubiose with cellulose hydrolysis.Hemicellulase becomes few pentosan and pentosan with hydrolysis of hemicellulose.Zytase becomes few xylan and wood sugar with xylan hydrolysis.
If identified two or more pollutants, should check the optimal pH and the temperature of used each enzyme, if possible, these enzymes can be used as multienzyme complex and add together.When two kinds of enzymes are united when using, seldom might two kinds enzyme all they optimal pH and temperature conditions under be used.In this case, can adopt two kinds of enzymes all to have a highly active relatively total overlapping region.If the pH of these two or more enzymes and temperature can one by one add they so just when very big-difference is arranged.Usually, how sharp these two kinds or enzyme usage rate be approximately identical with the pollutant ratio.The example of multienzyme complex comprises following but is not limited to these:
Carbohydrase comprises arabanase, cellulase, 1,4 beta-glucanase, hemicellulase and zytase (Viscozyme)
AMS, 1,4 beta-glucanase, protease (Ceremix)
Those skilled in the art will recognize that those skilled in the art can be used for the present invention with these enzymes along with progress of research and evaluation with enzyme of bigger tolerance (particularly low pH scope in) are come out.
In another preferred embodiment of the present invention, before enzymatic treatment, carry out the evaluation of pollutant.Although this step is not to clean the sin qua non of institute, it can save money and time, avoids the use and the unsuccessful cleaning of invalid enzyme.Because enzyme all has the selectivity of height to their substrate usually, so ideal situation is after pollutant is identified, just to select the enzyme or the enzymatic mixture that are used for handling this resin.Yet, one skilled in the art will appreciate that the source of determining pollutant in the resin is relatively more difficult sometimes, or impossible.Therefore, the selection of enzyme must be carried out under the uncomprehending situation to pollutant sometimes.
In another preferred embodiment of the present invention, before handling, the pH of resin is transferred in the optimal pH scope of selected enzyme with one or more enzymes.
If to be processed is resin cation, the capacity applications NaOH of resin or Na
2CO
3Exhaust the level that reaches 6-8 until pH fully, the suitableeest is 6.5-7.5.If use Na
2CO
3Exhaust resin, must take more care, because in this unit, can form CO
2Thereby, improved the gross pressure in the pipe.
After removing sweet (sweetening off), just can handle weak or strongly basic anionic resin.If pH is greater than 7.5 in the pipe, uses HCl and finish exhausting of resin.
In another preferred embodiment of the present invention, before removing the pollutant initial concentration that is still reacting (as ion-exchange), thoroughly clean resin with cleaning agent (as water) with one or more enzymes.In the wet milling industry of carbohydrate and cereal, this step is called as " going sweet " (" sweeteningoff ").In this application, go sweet being defined as, carry out chemistry and or before enzyme handles, from resin and pipeline, wash syrup, sugar, wine or other liquid based on carbohydrate, total organic carbon in pipeline or unit is below the 3500ppm 3500/1000000ths, more preferably below the 2500ppm.After being defined in " going sweet ", remaining syrup is retained in ducted syrup.In this application, " initial concentration of pollutant " is restricted to pollutant load greater than 2500ppm, more preferably 1000ppm, also more preferably 100ppm.Among the application, " residual volume of pollutant " is restricted to amount of pollutant and is less than 2500ppm, more preferably 1000ppm, also more preferably 100ppm.
This cleaning agent is water and does not require it is drinkable, although the purest preferred water.Randomly, salt can be added in the entry to assist " going sweet " process of being somebody's turn to do.Preferably, water should be softened, and perhaps, possible words should be used the condensed water that reclaims flash-pot.In carbohydrate and the industry of cereal wet milling, what select for use is condensed water.
Resin is exposed in this cleaning agent, is eliminated until the residual pollutant initial amount that carries out ion-exchange.In carbohydrate and the industry of cereal wet milling, this cleaning of carrying out with cleaning agent or " going sweet " process must last till stay in this unit can detected total organic carbon (total organiccarbon, TOC) the content maximum is no more than 2000ppm, more preferably content is less than 1000ppm, and also more preferably content is less than 100ppm.For the application's purpose, " total organic carbon " (TOC) is defined as the measured value of all organic carbons in the sample.Because be the final oxidation of measuring organic carbon with the form of carbon dioxide, so the small sample that burns is measured the carbon dioxide content that sheds, therefrom deduct the amount that DIC causes again in fuel flask, just can determine total content of organic carbon.Be retained in total organic carbon in this unit or these content of residual sugar and would not disturb cleaning process.Content just might disturb this process greater than 2000ppm.The use of cleaning agent also makes those skilled in the art handle the temperature that can regulate resin before the pollutant with one or more enzymes.The temperature of used cleaning agent should or the optimum temperature range of a little higher than selected enzyme because the adjusting of pH can reduce temperature in the pipeline.
In another preferred embodiment of the present invention, after finishing dealing with, wash this enzyme solutions enzyme concentration in pipe with a kind of solution (normally water) and be rinsed totally.
In another preferred embodiment of the present invention, resin is regenerated with the chemical step of knowing.The typical concentration and the chemicals that are used to regenerate comprise: to the weakly-basic anion type of resin, with 4%NaOH solution (96-112kg/m
3) and 5%Na
2CO
3Solution (112-118kg/m
3) 40 ℃ of recirculation 30-60 minute.To strongly basic anionic resin, with 4% sodium hydroxide solution (112-120kg/m
3) and 7% sodium carbonate liquor (80-96kg/m
3) 40 ℃ of recirculation 30-60 minute.
Be to comprehensive description of the present invention above, its content can be with reference to some embodiment and understand better, and these included herein embodiment only are used for illustrating the present invention, should not be construed as limiting the present invention.
Embodiment
The mensuration program of pollutant in embodiment 1. resins
If before selection is used for handling the enzyme of pollutant and handles, just identify the type of resin pollutant, just can save the time and money of unnecessary cost.Carry out Analysis of contaminant with conventional method.Normally, one skilled in the art will realize that such as following resin stain thing: protein; Lipid is as fat and oily; Fiber such as cellulose, hemicellulose; Beta glucan; Starch such as amylose, amylopectin; Or other carbohydrate, as arabose or xylanose.Those skilled in the art's following steps commonly used are identified existing pollutant in the resin.After should in bottle, filtering resin all contaminants is analyzed.Only solution is measured.Measure normally strict, and comprise with the strong oxidizer of resin reaction.
2 gram resin sample are put in the beaker, and an existing magnetic stirring bar adds entry to cumulative volume 200ml in the beaker.The pH of regulator solution and temperature are in the optimal pH scope of selected enzyme.This enzyme is weighed in advance so that its total percentage in solution is equivalent to the 3-5% of this total solution weight.The purpose in this step is to determine the composition of pollutant.Each sample is only checked with a kind of enzyme.With this solution mixing 30-60 minute, analyze the source of polluting then rightly.
Available many kinds of method spaces are analyzed, and wherein most of method is " standard method " in the special industry.For used resin in cereal wet milling factory or the carbohydrate refinery, most probable pollutant is: protein, starch, beta glucan, fat and lipid, wherein protein is maximum.
The analysis of protein can be finished with the Kai Shi method, and this method is from the standard method of analysis of corporator of cereal refiner association: cereal starch (unmodified), method B-48.Kjeldahl method has many variations, but this method is suitable, and very sensitive.
The available natural fat of analysis (the available hexane extracting) method of fat and lipid is finished, and this method is from the standard analytical process of the Zhu Chengyuan of company of cereal refiner associating association; Feed, method G-11.This program has other to alter the procedure, but this method is the most suitable.
Available this natural fiber method of the analysis of beta glucan (cereal fiber) is finished, and this method is from the standard analytical process of the Zhu Chengyuan of company of cereal refiner associating association; Feed, method G-12.This program has other to alter the procedure, but this method is the most suitable.
Available this natural fiber method of the analysis of beta glucan (cereal fiber) is finished, and this method is from the standard analytical process of the Zhu Chengyuan of company of cereal refiner associating association; Feed, method G-12.This program has other to alter the procedure, but this method is the most suitable.
Available this natural fiber method of the analysis of beta glucan (cereal fiber) is finished, and this method is from the standard analytical process of the Zhu Chengyuan of company of cereal refiner associating association; Feed, method G-12.This program has other to alter the procedure, but this method is the most suitable.
The analysis of starch can be finished by several standard iodine solution are added in this resin solution.If behind the adding iodine solution, form purple, green or blue in the flask, so wherein there is the starch of native form.Embodiment 2. enzyme treatment conditions
Pollutant can optionally be identified with the method for embodiment 1.Although this is unessential, it makes and can suitably select the enzyme of handling pollutant, and can select suitable chemical treatment method for use.Polluting total amount recently estimates and represents with accounting for percentage that resin adds the pollutant gross weight.Use cleaning agent, cleaning is gone sweet to the ducted resin of this ion-exchange as water, until amounts of residual contamination do or pollutant levels (material of experience ion-exchange), be reduced to acceptable level as cereal syrup or high sugared cereal syrup, normally the 1000-2500ppm total organic carbon.Although lower level is better.
The pH that regulates resin is to the pH scope of selected enzyme.For example, if selecting bacteria protease, then this pH scope is 5-9, and more preferably pH5.5-7.5, and temperature range is 20-75 ℃, more preferably 40-60 ℃.If select plant rennet, as papain, then the pH scope is 2.5-10, and preferably pH3-9.5, and temperature range is 30-60 ℃, more preferably 38-55 ℃.
The chemicals that is used to regulate pH includes but are not limited to: NaOH, hydrochloric acid, ammonia and sodium carbonate.Suitable enzyme mixed with water again add in the pollutant.Its dosage depends on the type and the degree of pollution.This enzyme is by one section reasonable time of ion-exchange pipeline recirculation.RCT depend on used enzyme, with the degree of closeness and the pollution level of the suitableeest enzyme condition.Normally 1-24 hour circulation timei.After finishing processing, wash this enzyme solutions with a kind of solution (normally water), enzyme content is dashed to the greatest extent in pipe.This moment, resin was optionally regenerated with the chemical step of standard.The typical concentration and the chemicals that are used for regenerating resin comprise: to weakly-basic anion type resin, with 4% sodium hydroxide solution (96-112kg/m
3) and 5% sodium carbonate liquor (112-118kg/m
3), 40 ℃ of recirculation 30-60 minute.To strongly basic anionic resin, with 4% NaOH (112-120kg/m
3) and 7% sodium carbonate liquor (80-96kg/m
3), 40 ℃ of recirculation 30-60 minute.The enzymatic treatment of embodiment 3. cereal gluten-contamination things
Present embodiment has been set forth and has been used for the method for removing cereal gluten-contamination thing from cation or anion exchange resin, and this protein can not be handled or water be removed with chemicals.
At first resin is handled with the traditional chemical method.The step of this chemical test flushing is, the NaOH circulation that cycles through resin anion (R.A.) (Dowex88) and 4% (1N) with 7% hydrochloric acid (1N) is by resin anion (R.A.) (Dowex66), and the time is 4 hours, and temperature is 120F, and this is similar to plant conditions.Rinse out the chemicals in the post, suitably adjust irrigation tests subsequently in order to enzyme.
Water flushing (going sweet) resin is adjusted to the suitableeest enzyme operative temperature.Regulate pH to 6.8 in the pipeline with 5% sodium carbonate liquor, this is near the optimal pH of enzyme.
Used enzyme is 0.5L peptase (Neutrase).Every 1500ft
3Add the 30kg peptase in the resin.This enzyme content is estimated as 1% of the total amount of pollutant.This enzyme is added into by sucking cation and anion pipeline.This pipeline is passed through in this solution recirculation, long 4 hours of total cycle time.From pipe, rinse out this enzyme subsequently.Analyze the total protein content in the enzyme sample wash with standard method." Kai Shi analyzes (the cereal refiner member's of association standard method of analysis, method B-48) ".1 hour collection analysis solution of every circulation obtains following result.Deducted the albumen quality that can be detected as the enzyme of protein in the sample.Protein % in the protein % solution in the time solution
(only wash) (cleaning) 1 0.2% 0.5%2 0.3% 0.8%3 0.7% 1.1%4 0.7% 1.4% with enzyme with HCl and NaOH
Enzyme added in 5 minutes, and solution is muddy tan appearance in the pipeline.This effect is extremely significant, because before carrying out the enzyme processing, can both see the protein that exists in the pipe across the visual glass of pipe.After through two kinds of processing, can't see the protein piece again, and reduced by 70% the action time of resin unit, be returned to normal.From then on can be clear that among the embodiment that the enzyme facture is more much effective than method of chemical treatment used in the prior art.The efficient of enzyme facture wants high by 100%.
Claims (31)
1. remove a kind of enzymatic method of one or more pollutants from resin, this method comprises with one or more enzymes handles this resin.
2. the enzymatic method of claim 1, it also is included in before enzyme handles, and removes one or more pollutants of initial concentration with irrigation from resin, makes pollutant reach residual level.
3. the enzymatic method of claim 1, it comprises that also the pH that regulates resin is to adapt to the optimal pH scope of one or more enzymes.
4. the enzymatic method of claim 1, it further is included in enzyme and handles the back and removes one or more enzymes with second kind of cleaning agent from resin.
5. the method for claim 2, wherein the amount of handling the residual contaminants that the back exists with cleaning agent is 2500ppm or still less.
6. the method for claim 2, wherein the amount of handling the residual contaminants that the back exists with cleaning agent is 1000ppm or still less.
7. the process of claim 1 wherein that the amount of handling the residual contaminants that the back exists with cleaning agent is 100ppm or still less.
8. the method for claim 2, wherein said cleaning reagent is a water.
9. a kind of enzyme that the process of claim 1 wherein is a kind of protease.
10. a kind of enzyme that the process of claim 1 wherein is a kind of 1,4 beta-glucanase.
11. a kind of enzyme that the process of claim 1 wherein is a kind of lipase.
12. a kind of enzyme that the process of claim 1 wherein is a kind of AMS.
13. a kind of enzyme that the process of claim 1 wherein is a kind of carbohydrase.
14. the process of claim 1 wherein the enzyme that has used two or more.
15. the method for claim 13 is wherein with two or more enzyme process resin that are selected from following member: protease, 1,4 beta-glucanase, lipase, AMS and carbohydrase.
16. the method for claim 15, wherein said two kinds of enzymes are AMS and 1,4 beta-glucanase.
17. the method for claim 4, wherein the optimal pH scope is between 1-9.5.
18. the method for claim 4, wherein the optimal pH scope is between 3-8.
19. the method for claim 4, wherein the optimal pH scope is between 6-8.
20. the method for claim 4, wherein the optimal pH scope is between 6.5-7.5.
21. the process of claim 1 wherein that described resin is a kind of resin anion (R.A.).
22. the method for claim 21, wherein said resin anion (R.A.) are a kind of highly basic.
23. the method for claim 21, wherein said resin anion (R.A.) are a kind of strong acid.
24. the process of claim 1 wherein that described resin is a kind of resin cation.
25. the method for claim 24, wherein said resin cation are a kind of strong acid.
26. the method for claim 24, wherein said resin cation are a kind of highly basic.
27. the process of claim 1 wherein that described resin has also carried out chemical treatment.
28. the method for claim 27, wherein said chemical treatment is carried out with hydrochloric acid, NaOH or sodium carbonate.
29. the process of claim 1 wherein that described resin is a kind of ion exchange resin.
30. the process of claim 1 wherein that described resin is a kind of fractionation resin.
31. a method of producing the cereal sweetener is wherein crossed described cereal sweetener a kind of resin of having handled with enzyme, is purified until this cereal sweetener.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2286796P | 1996-07-30 | 1996-07-30 | |
| US60/022,867 | 1996-07-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1226843A true CN1226843A (en) | 1999-08-25 |
Family
ID=21811857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97196893A Pending CN1226843A (en) | 1996-07-30 | 1997-07-03 | Enzymatic method for removing contaminants from ion exchange and fractionation resin |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0915734A1 (en) |
| JP (1) | JP2000516709A (en) |
| CN (1) | CN1226843A (en) |
| AU (1) | AU3960197A (en) |
| WO (1) | WO1998004344A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105332149A (en) * | 2015-10-21 | 2016-02-17 | 嵊州市中森电子有限公司 | Tension-controllable weft storage unit |
| CN107922216A (en) * | 2015-09-15 | 2018-04-17 | 陶氏环球技术有限责任公司 | Method for regenerating acrylic resin |
| CN112844345A (en) * | 2020-12-29 | 2021-05-28 | 无锡中天固废处置有限公司 | Method for treating solid waste after starch wastewater is adsorbed by activated carbon |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5759641A (en) * | 1980-09-26 | 1982-04-10 | Japan Organo Co Ltd | Regenerating method for strong acidic cation exchange resin |
| DE59408670D1 (en) * | 1993-02-12 | 1999-10-07 | Filtrox Ag | Process for cleaning a filter aid by adding enzymes |
-
1997
- 1997-07-03 WO PCT/US1997/012591 patent/WO1998004344A1/en not_active Application Discontinuation
- 1997-07-03 CN CN97196893A patent/CN1226843A/en active Pending
- 1997-07-03 AU AU39601/97A patent/AU3960197A/en not_active Abandoned
- 1997-07-03 JP JP10508890A patent/JP2000516709A/en active Pending
- 1997-07-03 EP EP97936972A patent/EP0915734A1/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107922216A (en) * | 2015-09-15 | 2018-04-17 | 陶氏环球技术有限责任公司 | Method for regenerating acrylic resin |
| CN107922216B (en) * | 2015-09-15 | 2021-06-08 | 陶氏环球技术有限责任公司 | Method for regenerating acrylic resin |
| CN105332149A (en) * | 2015-10-21 | 2016-02-17 | 嵊州市中森电子有限公司 | Tension-controllable weft storage unit |
| CN105332149B (en) * | 2015-10-21 | 2018-05-29 | 泉州市新空间装饰工程有限公司 | A kind of controllable weft accumulator of tension |
| CN112844345A (en) * | 2020-12-29 | 2021-05-28 | 无锡中天固废处置有限公司 | Method for treating solid waste after starch wastewater is adsorbed by activated carbon |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998004344A1 (en) | 1998-02-05 |
| EP0915734A1 (en) | 1999-05-19 |
| AU3960197A (en) | 1998-02-20 |
| JP2000516709A (en) | 2000-12-12 |
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