CN1220824A - Preserving fluid for skin - Google Patents
Preserving fluid for skin Download PDFInfo
- Publication number
- CN1220824A CN1220824A CN 98118175 CN98118175A CN1220824A CN 1220824 A CN1220824 A CN 1220824A CN 98118175 CN98118175 CN 98118175 CN 98118175 A CN98118175 A CN 98118175A CN 1220824 A CN1220824 A CN 1220824A
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- China
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- skin
- preserving fluid
- sulfoxide
- methyl
- antifreeze
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Abstract
A skin preserving liquid contains balancing liquor, dimethylsulfoxide (10-20 vol.%), antifreezing agent (one or two of 1,2-propanediol, 1,3-propanediol, and propane triol or their mixture) (0.5-20 vol.%), efficient penetrant (nitrone) (0.5-10 vol.%), and optional anionic surfactant (sodium lauryl sulfate), and featuers long preservation period, high activity of preserved sking and high survival rate of the skin.
Description
The invention belongs to the preservation technical field of human or animal body or its part, particularly a kind of snap frozen profound hypothermia human body skin stores uses preserving fluid for skin.
The method that is used at present to store human body skin both at home and abroad has ultralow temperature skin preservation method and slow cooling to freeze the skin preservation method, the former be the skin that will process after methyl-sulfoxide is handled, be stored in ultra low temperature freezer (80 ℃) or CO
2In the dry ice; The latter then be the human body skin that processes after methyl-sulfoxide is handled, be stored in ultra low temperature freezer or CO
2In the dry ice, be cooled to-72 ℃, be stored into again after 12 hours in-196 ℃ of liquid nitrogen and preserve with the speed of 1-3 ℃/min.Above-mentioned two kinds of methods are used for that problem that human body skin preserve to exist mainly is that the preserving fluid for skin frost resistance is poor, poor permeability, Skin Cell frost resistance are inhomogeneous, and crystallization rate is inconsistent inside and outside the Skin Cell in storage process, causes in the refrigerating process damage to Skin Cell.The skin vitality of result through storing be low, it is low to transplant the back survival rate, and with skin holding time weak point, the complex operation of these two kinds of methods preservations, waste time and energy, need equipment is many, cost is high.
The object of the present invention is to provide a kind of good preserving fluid for skin of skin effects of preserving.
For reaching above-mentioned purpose, the present invention adopts following technical scheme: a kind of preserving fluid for skin, contain equilibrium liquid, methyl-sulfoxide, antifreeze, also contain high-efficient penetrant, wherein methyl-sulfoxide is 10-20% by volume, and antifreeze is 0.5-20%, and high-efficient penetrant is 0.5-10%.
Methyl-sulfoxide is 15-20%, and antifreeze is 5-10%, and high-efficient penetrant is 0.5-5%.
Antifreeze is 1,2-propane diols, 1, and one of ammediol, glycerine or two or three mixture, high-efficient penetrant is an azone.
The anion surfactants such as sldium lauryl sulfate that also contain trace.
A kind of preparation method of preserving fluid for skin of the present invention is: with methyl-sulfoxide, antifreeze and high-efficient penetrant (etc.) fully mix the back and add mixing in the equilibrium liquid, transfer PH=6.8-7.8, equilibrium liquid is equilibrium liquid medically commonly used, equilibrium liquid as following composition: sodium chloride 0.52 gram, potassium chloride 0.027 gram, calcium chloride 0.021 gram, magnesium sulfate 0.022 gram, sodium hydrogen phosphate 0.414 gram, 1N hydrochloric acid 0.23ml, mix adding distil water to 100ml.
Use the present invention to store skin bit, at first satisfactory human body skin is processed into the thick skin bit of 0.35-0.6mm under aseptic condition, be dipped in the preserving fluid for skin of the present invention under the 0-4 ℃ of temperature, soaked 20-40 minute, be cut into 750-2500CM
2Size is gone into aseptic plastic bag, is sealed, and the storage skin groove of packing into, input liquid nitrogen are preserved.When need using skin, the plastic sack that skin bit is housed was put in 40 ℃ of aqueous solution water-bath vibration or eddy flow rewarming 3 minutes, the antibacterials immersion got final product in 10 minutes.
The present invention fully takes into account the toxicity and the penetrating odor of methyl-sulfoxide, has adopted the suitable concentration of 10-20% (V/V); In preserving fluid for skin, add antifreezes such as propane diols, glycerine, improve the skin frost resistance; Adopt the bleeding agent such as the azone (1-azone-2) of high effect nontoxic, improved the seepage velocity of preserving liquid greatly.Make and preserve liquid and can infiltrate into equably rapidly inside and outside the Skin Cell, in the stored frozen process because the inside and outside crystallization rate uniformity of cell, avoided freezing to greatest extent and the rewarming process to the damage of Skin Cell, the skin holding time is prolonged.Slow freezing or-80 ℃ of low temperature refrigerator preservation methods are preserved the time of skin generally at 6 months to 1 year, after for a long time then skin vitality greatly descend, and the skin that the present invention preserves after liquid is handled can store in liquid nitrogen for a long time, in our clinical practice, the dermatoplasty survival rate that stores after 3 years is still very high, utilize general biological oxygen consumption skin vitality analyzer (the DBM-I type in this area, Beijing 304 hospitals produce) measure and show storing afterwards the skin bit vigor with the succinate dehydrogenase determination method, freeze or-80 ℃ of refrigerator preservation methods than slow cooling, the present invention preserves liquid and handles skin storage back, back vigor apparently higher than the former, improve 25-37.1%, reach more than 90%.The dermatoplasty survival rate height that stores reaches more than 96%.Skin after adopting the present invention to preserve liquid in addition to handle carries out that freezing processing speed is fast, method simple, saves time, laborsaving, province's equipment.
Up to now, we reach 860,000 CM with the method preservation skin
2More than, be widely applied to ground such as Zhengzhou, Anyang, Puyang, Xuchang, Jiaozhuo, be applied to 90% large-area burns of the gross area 100% 3 degree, the quick-frozen allograft skin survives fully.The present invention lays the foundation for the successful rescue of countless extensive deep burns, and the patient has brought Gospel for large-area burns.
Further specify the present invention below by embodiment:
The each component proportioning sees the following form among the embodiment 1,2,3,4,5,6, its preparation method is: the preparation equilibrium liquid, sodium chloride 0.52 gram, potassium chloride 0.027 gram, calcium chloride 0.021 gram, magnesium sulfate 0.022 gram, sodium hydrogen phosphate 0.414 gram, 1N hydrochloric acid 0.23ml mixes the back adding distil water to 100ml, autoclaving, get methyl-sulfoxide, anti frozen liquid, high-efficient penetrant (etc.) mix, add in the cooled equilibrium liquid, transfer PH to 6.8-7.8.
| Embodiment composition (V/V%) | ????1 | ????2 | ????3 | ????4 | ????5 | ????6 |
| Methyl-sulfoxide | ????10 | ????10 | ????15 | ????15 | ????15 | ????20 |
| 1, the 2-propane diols | ????5 | ????10 | ????0 | ????0 | ????5 | ????0 |
| 1, ammediol | ????0 | ????0 | ????5 | ????10 | ????0 | ????10 |
| Glycerine | ????5 | ????0 | ????0 | ????5 | ????5 | ????10 |
| Azone | ????6 | ????5 | ????5 | ????1 | ????2 | ????0.5 |
| Equilibrium liquid | Surplus | Surplus | Surplus | Surplus | Surplus | Surplus |
Claims (4)
1, a kind of preserving fluid for skin contains equilibrium liquid, methyl-sulfoxide, antifreeze, and the present invention is characterised in that, also contains high-efficient penetrant, and wherein methyl-sulfoxide is 10-20% by volume, and antifreeze is 0.5-20%, and high-efficient penetrant is 0.5-10%.
2, preserving fluid for skin as claimed in claim 1 is characterized in that, methyl-sulfoxide is 15-20%, and antifreeze is 5-10%, and high-efficient penetrant is 0.5-5%.
3, preserving fluid for skin as claimed in claim 1 or 2 is characterized in that, antifreeze is 1,2-propane diols, 1, and one of ammediol, glycerine or two or three mixture, high-efficient penetrant is an azone.
4, preserving fluid for skin as claimed in claim 3 is characterized in that, also contains the anion surfactants such as sldium lauryl sulfate of trace.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98118175 CN1220824A (en) | 1998-09-10 | 1998-09-10 | Preserving fluid for skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98118175 CN1220824A (en) | 1998-09-10 | 1998-09-10 | Preserving fluid for skin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1220824A true CN1220824A (en) | 1999-06-30 |
Family
ID=5225942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98118175 Pending CN1220824A (en) | 1998-09-10 | 1998-09-10 | Preserving fluid for skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1220824A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101296691B (en) * | 2005-08-05 | 2011-07-27 | 努沃研究公司 | Transdermal drug delivery formulation |
| CN103004752A (en) * | 2013-01-18 | 2013-04-03 | 江西省科星生物工程有限公司 | Skin preserving fluid with mycose as raw material, preparation method of skin preserving fluid and skin preservation method |
| CN108094410A (en) * | 2018-01-26 | 2018-06-01 | 山东大学齐鲁医院 | A kind of skin profound hypothermia protective agent and vitrification method |
| CN108782544A (en) * | 2018-06-28 | 2018-11-13 | 吉林省拓华生物科技有限公司 | A kind of a large amount of frozen stock solutions and method for freezing mescenchymal stem cell |
| CN111937859A (en) * | 2020-07-13 | 2020-11-17 | 北京利物谱生物科技有限公司 | Serum-free skin cryopreservation liquid and application thereof |
-
1998
- 1998-09-10 CN CN 98118175 patent/CN1220824A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101296691B (en) * | 2005-08-05 | 2011-07-27 | 努沃研究公司 | Transdermal drug delivery formulation |
| CN103004752A (en) * | 2013-01-18 | 2013-04-03 | 江西省科星生物工程有限公司 | Skin preserving fluid with mycose as raw material, preparation method of skin preserving fluid and skin preservation method |
| CN108094410A (en) * | 2018-01-26 | 2018-06-01 | 山东大学齐鲁医院 | A kind of skin profound hypothermia protective agent and vitrification method |
| CN108094410B (en) * | 2018-01-26 | 2021-08-31 | 山东大学齐鲁医院 | Skin deep hypothermia protective agent and skin preservation method |
| CN108782544A (en) * | 2018-06-28 | 2018-11-13 | 吉林省拓华生物科技有限公司 | A kind of a large amount of frozen stock solutions and method for freezing mescenchymal stem cell |
| CN108782544B (en) * | 2018-06-28 | 2021-01-01 | 吉林省拓华生物科技有限公司 | Cryopreservation solution and method for cryopreservation of large amount of mesenchymal stem cells |
| CN111937859A (en) * | 2020-07-13 | 2020-11-17 | 北京利物谱生物科技有限公司 | Serum-free skin cryopreservation liquid and application thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |