CN1219073C - Ultraviolet laser biological particle counter - Google Patents
Ultraviolet laser biological particle counter Download PDFInfo
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- CN1219073C CN1219073C CN02136858.9A CN02136858A CN1219073C CN 1219073 C CN1219073 C CN 1219073C CN 02136858 A CN02136858 A CN 02136858A CN 1219073 C CN1219073 C CN 1219073C
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- fluorescence
- scattered light
- ultraviolet laser
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
An ultraviolet laser biological particle counter is suitable for measuring the diameter and biological characteristics of suspended biological particles in air. The device comprises an ultraviolet laser illumination light path, a scattered light receiving light path, a fluorescence receiving light path and a sampling gas path, wherein the ultraviolet laser illumination light path takes an ultraviolet laser as an illumination light source. The scattered light filter arranged on the scattered light receiving light path totally reflects the fluorescence, so that the intensity of the fluorescence received by the fluorescence receiving light path is increased by 1 time. The fluorescent filter arranged on the fluorescent receiving light path totally reflects the ultraviolet laser beam, so that the intensity of the ultraviolet laser received on the scattered light receiving light path is increased by 1 time. Compared with the prior art, the particle counter has multiple measurement functions, and can measure not only the size of suspended particles, but also the fluorescence intensity. The receiving solid angle of the photosensitive area is larger, the detection sensitivity is high, the receiving efficiency is higher, and the signal-to-noise ratio is higher.
Description
Technical field:
The present invention relates to a kind of ultraviolet laser biomone counter, be mainly used in diameter and the biological characteristics thereof of measuring suspended biological particle in the air.
Background technology:
Float on airborne suspended particles, abiotic particle is arranged; Biomone is also arranged, as planktons such as bacterium and cenobiums.The biomone cell contains fluorescence molecules such as riboflavin, Triphosphopyridine nucleotide, reduced and tryptophane, under the irradiation of the Ultra-Violet Laser between 320～360nm, will produce scattered light and fluorescence at the very high wavelength of photon energy simultaneously.The scattering light wavelength is identical with the wavelength of ultraviolet laser illuminator, and the wavelength of fluorescence is greater than the wavelength of ultraviolet laser illuminator, and its wavelength region is between 390～600nm.Determine its geometrical dimension in real time by the scattered light intensity of measuring the suspended biological particle, and determine its biological characteristics in real time by measuring its fluorescence intensity.
Formerly in the technology, the patent No. that is provided by Sujing Group Co., Suzhou City is ZL97241940.3 " the micro-optical transmitter of laser dust particle counter ", as shown in Figure 1.This optical pickocff mainly is made up of orthogonal illumination path and scattered light receiving light path.Illumination path wherein is made up of laser diode 1, preceding convex lens 2, back convex lens 3, concavees lens 4, photosensitive area 5 and light trapping 7; Has only photorectifier 6 on the scattered light receiving light path.
There is following shortcoming in this optical pickocff:
1. measurement function is single, can only measure the geometrical dimension of suspended particles.If by photosensitive area 5 be the suspended biological particle, then remove scatter with the lighting light wave appearance with scattered light signal the time, also can produce the fluorescence of wavelength greater than the illumination light wavelength.And this optical pickocff receives only scattered light, so can only judge its size by measuring scattered light intensity, can't obtain its biological characteristics.
2. signal to noise ratio is low, and is low to the detectivity of suspended particles.Reason is as follows: the first, this optical pickocff illumination path laser beam that laser diode 1 is penetrated becomes behind preceding convex lens 2, back convex lens 3, concavees lens 4 and is similar to parallel light photosensitive area 5, so the illuminating bundle cross-sectional area is big, photosensitive area 5 illumination light power densities are little, cause signal to noise ratio low; The second, have only photorectifier 6 on the scattered light receiving light path, it is little to the reception solid angle of photosensitive area 5, cause the receiving efficiency of scattered light lower, so a little less than the signal, signal to noise ratio is lower; Three, photorectifier 6 receiving scattered lights the time, also can receive the diffraction veiling glare from illuminating laser beam, cause optical noise big, signal to noise ratio is low.
Summary of the invention:
The invention provides a kind of ultraviolet laser biomone counter, determine its geometrical dimension by the scattered light intensity of measuring the suspended biological particle, and determine its category by measuring its fluorescence intensity.The present invention contains three light paths and a sampling gas circuit.Article three, light path is Ultra-Violet Laser illumination path, scattered light receiving light path and fluorescence receiving light path, is respectively applied for the tested suspended particles of illumination, receives scattered light and fluorescence from tested suspended particles.After scattered light receiving light path and fluorescence receiving light path, have respectively corresponding electronic circuit and computer with processing signals, provide measuring result.
Concrete structure of the present invention comprises three light paths as shown in Figures 2 and 3: Ultra-Violet Laser illumination path, scattered light receiving light path and fluorescence receiving light path.The optical axis of Ultra-Violet Laser illumination path is O
1O ', the optical axis of scattered light receiving light path are OO
2, the optical axis of fluorescence receiving light path is OO
3, and scattered light receiving light path optical axis OO
2With fluorescence receiving light path optical axis OO
3On same straight line, again with the optical axis O of Ultra-Violet Laser illumination path
1O ' is vertically intersected on the central point O of photosensitive area 5.Scattered light receiving light path and fluorescence receiving light path are positioned at the both sides of photosensitive area 5.On the Ultra-Violet Laser illumination path, be equipped with ultraviolet laser 8, beam expanding lens group 9, cylindrical mirror 10 and light trapping 7 successively.Photosensitive area 5 is between cylindrical mirror 10 and light trapping 7, and the focus O of cylindrical mirror 10 overlaps with the central point O of photosensitive area 5.
On the scattered light receiving light path, between photosensitive area 5 and scattered light receptor 13,5 beginnings are with optical axis OO from the photosensitive area
2Be equipped with scattered light successively and receive mirror group 11 and scattered light diaphragm 12, wherein scattered light reception mirror group 11 is made up of first collimating mirror 111, scattered light spectral filter 112 and first condensing lens 113, and scattered light spectral filter 112 places between first collimating mirror 111 and first condensing lens 113.The front focus O of first collimating mirror 111 overlaps with the central point O of photosensitive area 5, and scattered light diaphragm 12 places the back focus O of first condensing lens 113
2On.The output of scattered light receptor 13 links to each other with computer 21 through scattered light amplifier 14.
On the fluorescence receiving light path, between the fluorescence receptor 17,5 beginnings are with optical axis OO from the photosensitive area in photosensitive area 5
3Ground is equipped with fluorescence successively and receives mirror group 15 and fluorescence diaphragm 16, and wherein fluorescence reception mirror group 15 is made up of second collimating mirror 151, fluorescent optical filter 152 and second condensing lens 153, and fluorescent optical filter 152 places between second collimating mirror 151 and second condensing lens 153.The front focus O of second collimating mirror 151 overlaps with the central point O of photosensitive area 5, and fluorescence diaphragm 16 places the back focus O of second condensing lens 153
3On.The output of fluorescence receptor 17 links to each other with computer 21 with photon counter 20 through peak-holding circuit 19 respectively after amplifying by fluorescence amplifier 18.
Contain the inlet pipe 22 and the vapor pipe 23 that place 5 relative both sides, photosensitive area on the sampling gas circuit among the present invention, its central axis O
4O " pass the central point O of photosensitive area 5, perpendicular to by Ultra-Violet Laser illumination path optical axis O
1O ', scattered light receiving light path optical axis OO
2With fluorescence receiving light path optical axis OO
3The plane that constitutes.As shown in Figure 3.
From structure iron 2,3 of the present invention and structure iron 1 comparison of technology formerly, characteristics of the present invention contain three light paths exactly: Ultra-Violet Laser illumination path, scattered light receiving light path and fluorescence receiving light path.On the Ultra-Violet Laser illumination path, between ultraviolet laser 8 and photosensitive area 5, be equipped with one dimension beam expanding lens group 9 and cylindrical mirror 10; On the scattered light receiving light path, between photosensitive area 5 and scattered light receptor 13, be equipped with scattered light and receive mirror group 11 and scattered light diaphragm 12, and scattered light reception mirror group 11 is made of first collimating mirror 111, scattered light spectral filter 112 and first condensing lens 113; On the fluorescence receiving light path, between photosensitive area 5 and fluorescence receptor 15, be equipped with fluorescence and receive mirror group 15 and fluorescence diaphragm 16, and fluorescence reception mirror group 15 is made of second collimating mirror 151, fluorescent optical filter 152 and second condensing lens 153.
Said beam expanding lens group 9 is made up of the recessed cylindrical mirror 901 of a short focal length and the protruding cylindrical mirror 902 of a long-focus, and it expands the bundle multiplying power between 4～6 times.The circular laser beam that it sends ultraviolet laser 8 is perpendicular to sampling gas circuit axes O
4O " expands bundle, makes it to become the oval-shaped laser bundle, and make the consistent size of laser illuminator area and photosensitive area 5 on the direction.
Said cylindrical mirror 10 is protruding cylindrical mirrors, and its in the photosensitive area 5, makes the laser beam line focus after expanding bundle through beam expanding lens group 9 illuminating laser beam at Ultra-Violet Laser illumination path optical axis O
1O ', scattered light receiving light path optical axis OO
2With fluorescence receiving light path optical axis OO
3Form a focal line in the plane that constitutes.The effect of Ultra-Violet Laser illumination path: the one, enlarged the lighting area of photosensitive area 5, guarantee that the tested suspended particles that flow through photosensitive area 5 all obtain illumination; The 2nd, reduce the cross-sectional area of photosensitive area 5 illuminating laser beams greatly, thereby improved the illumination light power density of photosensitive area 5.
Said scattered light receives mirror group 11 and is made of first collimating mirror 111, scattered light spectral filter 112 and first condensing lens 113.The front focus O of first collimating mirror 111 overlaps with the central point O of photosensitive area 5.Flow through scattered light that the tested suspended particles of photosensitive area 5 send and fluorescence by first collimating mirror, 111 collimations for behind the directional light, directive scattered light spectral filter 112.Scattered light spectral filter 112 is a kind of arrowband type bandpass filters, and its centre wavelength that sees through is positioned at the wavelength place of ultraviolet laser 8, and it is to the fluorescence total reflection, and Ultra-Violet Laser is played high as far as possible transmission effect, as shown in Figure 4.Scattered light spectral filter 112 by the photosensitive district 5 of former road reflected back, enters the fluorescence receiving light path of opposite side with fluorescence.The scattered light that sees through scattered light spectral filter 112 focuses on through first condensing lens 113 and places back focus O
2On scattered light diaphragm 12 on, project scattered light receptor 13 behind the filtering veiling glare.The characteristics that scattered light receives mirror group 11 are receiving scattered lights as much as possible, and the fluorescence receiving light path of fluorescence reflected back opposite side, make 1 times of the fluorescence intensity increase that the fluorescence receiving light path receives.
The subtended angle of 111 couples of photosensitive area 5 central point O of said collimating mirror is big, to the receiving efficiency height of scattered light and fluorescence.
Said fluorescence receives mirror group 15 and is made of second collimating mirror 151, scattered light spectral filter 152 and second condensing lens 153.The front focus O of second collimating mirror 151 overlaps with the central point O of photosensitive area 5.Flow through scattered light that the tested suspended particles of photosensitive area 5 send and fluorescence by second collimating mirror, 151 collimations for behind the directional light, directive fluorescent optical filter 152.Fluorescent optical filter 152 is a kind of long-pass type spectral filters, and it plays high as far as possible transmission effect to fluorescence, and the Ultra-Violet Laser less than wavelength of fluorescence is done up the total reflection effect, as shown in Figure 5.Fluorescent optical filter 152 with scattered light be UV laser beam by the photosensitive district 5 of former road reflected back, enter the scattered light receiving light path of opposite side.The fluorescence that sees through fluorescent optical filter 152 focuses on through second condensing lens 153 and places back focus O
3On fluorescence diaphragm 16 on, project fluorescence receptor 17 behind the filtering veiling glare.The characteristics that fluorescence receives mirror group 15 are to receive fluorescence as much as possible, and the scattered light receiving light path of scattered light reflected back opposite side, the scattered light intensity that the scattered light receiving light path is received increases by 1 times.
The subtended angle of 151 couples of photosensitive area 5 central point O of said collimating mirror is big, to the receiving efficiency height of scattered light and fluorescence.
Said scattered light amplifier 14 is after the electrical signal that is directly proportional with scattered light intensity of scattered light receptor 13 outputs is amplified, to send into computer 21.
After said fluorescence amplifier 18 amplifies the electrical signal that is directly proportional with fluorescence intensity of fluorescence receptor 17 output, send into peak-holding circuit 19 and photon counter 20 respectively after, input computer 21.The electrical signal of 21 pairs of peak-holding circuits 19 of computer and photon counter 20 outputs is gathered and is handled.The characteristics of electronic circuit of the present invention and computer are: the first, computer 21 is only when receiving the scattered light signal of scattered light amplifier 14 outputs, just the electrical signal of peak-holding circuit 19 and photon counter 20 outputs is gathered and handled, then scattered light signal and the fluorescent signal corresponding with it are saved in the internal memory of computer 21 as a data set; The second, peak-holding circuit 19 and photon counter 20 are handled fluorescent signal simultaneously.Because the fluorescence intensity transformation range of biomone is very big, the fluorescence of the biomone that has is extremely faint, has only several fluorescent photons; And the fluorescence intensity of the biomone that has is bigger, has the certain amplitude magnitude of voltage through obtaining one after amplifying.
The working process of ultraviolet biomone counter of the present invention is: the circular laser beam of sending by ultraviolet laser 8 through beam expanding lens group 9 perpendicular to sampling gas circuit axes O
4" after expanding Shu Chengwei oval-shaped laser bundle on the direction, in the photosensitive area 5, promptly the minor axis of oval-shaped laser bundle obtains compression to O, enters light trapping 7 then by cylindrical mirror 10 line focuss.Suspended particles to be measured flow through photosensitive area 5 with certain speed through inlet pipe 22, flow out from vapor pipe 23 then.When suspended particles flow through photosensitive area 5, produce and the proportional scattered light signal of its size,, then also can produce the fluorescent signal that is directly proportional with its contained fluorescence molecule quantity if suspended particles are biomones.With Ultra-Violet Laser illumination path optical axis O
1Scattered light signal on the vertical both direction of O ' in certain solid angle scope and fluorescent signal enter scattered light simultaneously and receive mirror group 11 and fluorescence reception mirror group 15, the fluorescent signal that enters scattered light reception mirror group 11 is scattered light spectral filter 112 and reflects back by former road behind collimating mirror 111 collimations, pass the fluorescence reception mirror group 15 that enters opposite side behind the photosensitive area 5; And the scattered light signal that enters fluorescence reception mirror group 15 is reflected back by former road by fluorescent optical filter 152 behind collimating mirror 151 collimations, passes the scattered light reception mirror group 11 that enters opposite side behind the photosensitive area 5.
Entering two beam spreadings that scattered light receives mirror group 11 penetrates optical signal and sees through scattered light spectral filter 112 backs and focused on scattered light diaphragm 12 by first condensing lens 113, be projeced into scattered light receptor 13 after passing scattered light diaphragm 12, the pulse height and the suspended particles size of the electric impulse signal of scattered light receptor 13 outputs are proportional.After computer 21 receives this electric impulse signal, at first provide the size of suspended particles according to its amplitude size; Simultaneously, to the peak-holding circuit 19 after the fluorescence amplifier 18 and photon counter 20 respectively output signal gather and handle.The two bundle fluorescent signals that enter fluorescence reception mirror group 15 see through fluorescent optical filter 152 backs and are focused on fluorescence diaphragm 16 by second condensing lens 153, be projeced into fluorescence receptor 17 after passing fluorescence diaphragm 16, the contained fluorescence molecule quantity of the electric impulse signal of fluorescence receptor 17 output and suspended particles is directly proportional.Computer 21 is kept at size value, the fluorescence intensity of suspended particles in the internal memory as a data set, and machine 21 to be calculated is handled, thereby finishes the measurement to suspended particle sizes and fluorescence intensity.
The present invention compares with technology formerly:
1. measurement function is many.The present invention adopts the ultraviolet laser 8 with high photon energy as lighting source, can measure the size and the fluorescence intensity of suspended particles simultaneously, promptly both can provide the geometrical dimension of tested suspended particles, can provide its biological characteristics again.
2. detectivity height.The first, Ultra-Violet Laser illumination path of the present invention adopt cylindrical mirror 10 with illuminating laser beam one dimension line focus in the photosensitive area 5, guaranteeing to have improved its illumination light power density greatly under the constant situation of photosensitive area 5 lighting areas, improved the launching efficiency of scattered light and fluorescence; The second, the numerical aperture of scattered light reception mirror group 11 and fluorescence reception mirror group 15 is big, to the receiving efficiency height of scattered light and fluorescence; Three, the scattered light spectral filter 112 in the scattered light receiving light path 11 reflexes to the fluorescence receiving light path with fluorescent signal, and the fluorescent optical filter 152 in the fluorescence receiving light path 15 reflexes to the scattered light receiving light path with scattered light signal, makes the present invention that the receiving efficiency of scattered light and fluorescence has all been improved 1 times again.Above-mentioned three technical measures have improved the detection signal to noise ratio of the present invention to suspended particles greatly, have promptly improved detectivity.
Description of drawings:
Fig. 1 is the structural representation of the micro-optical transmitter of technology laser dust particle counter formerly.
Fig. 2 be ultraviolet laser biomone counter of the present invention on y, x coordinate plane, three light path synoptic diagram.
Fig. 3 be ultraviolet laser biomone counter of the present invention on z, x coordinate plane, i.e. Ultra-Violet Laser illumination path and sampling gas circuit structure synoptic diagram on the A-A sectional plane among Fig. 2.
Fig. 4 is the spectral transmittance graphic representation of scattered light spectral filter 112 in the ultraviolet laser biomone counter of the present invention.
Fig. 5 is the spectrum transmitting rate curve of fluorescent optical filter 152 in the ultraviolet laser biomone counter of the present invention.
Embodiment:
Shown in the structure of Fig. 2 and 3.Ultraviolet laser 8 is that its wavelength is 355nm by all solid laser of the yttrium aluminum garnet of diode-end-pumped (YAG) laser apparatus behind frequency tripling, and beam diameter is φ 0.25mm, and power is 100mW.The expansion bundle multiplying power of beam expanding lens group 9 is 4 times, and promptly the cross section by the laser beam of beam expanding lens group 9 output is oval, and the length of its major axis and minor axis is respectively 1.0mm and 0.25mm.The focal length of cylindrical mirror 10 is 50mm, and 5 focal lines that form are of a size of 1.0mm * 0.173mm in the photosensitive area for it.Scattered light receives mirror group 11 and adopts identical structure with 151, two condensing lenss 113 with 153 with two collimating mirrors 111 that fluorescence receives in the mirror group 15, two collimating mirrors 111 and 151 focal length are 17.8mm, numerical aperture is 0.52, and the solid angle that photosensitive area 5 central point O are opened is 0.226 π steradian.Two condensing lenss 113 and 153 focal length are 32mm.The clear aperature of scattered light diaphragm 12 and fluorescence diaphragm 16 is 1.8mm * 0.32mm.The transmittance curve of scattered light spectral filter 112 as shown in Figure 4, its centre wavelength that sees through is 355nm, bandwidth is 46nm, the transmitance of central wavelength is greater than 58%.The transmittance curve of fluorescent optical filter 152 as shown in Figure 5, its for greater than the transmitance of 390nm wavelength light greater than 90%, and for having transmitance＜10 of the scattered light of identical wavelength with Ultra-Violet Laser
-6Scattered light receptor 13 and fluorescence receptor 17 are the side-on photomultiplier with multialkali photocathode material, have very high quantum yield in 180～910nm wave band.The inlet diameter of the exit diameter of inlet pipe 22 and vapor pipe 23 is respectively φ 1.0mm and φ 1.2mm.Scattered light amplifier 14 and fluorescence amplifier 18 all are to be the current/voltage-converted and the voltage amplifier circuit of core formation with high-performance field-effect cast dual operational amplifier.Peak-holding circuit 19 can keep the electrical signal peak that produces than the hyperfluorescence signal get off.The pulse signal that photon counter 20 detectable single photons produce.The electrical signal of 21 pairs of scattered light amplifiers 14 of computer, peak-holding circuit 19 and photon counter 21 outputs is gathered and is handled, and finally provides the geometrical dimension and the fluorescence intensity level of tested suspended particles.
It is 0.15 μ m that the minimum of embodiment is surveyed particle diameter, and the fluorescence intensity of minimum detectable is 1 photon.The air sampling flow is 5 liters/minute.
Claims (5)
1. a ultraviolet laser biomone counter comprises illumination path and receiving light path, it is characterized in that:
＜1〉said illumination path is to be the Ultra-Violet Laser illumination path of the ultraviolet laser (8) of 355nm as light source with the wavelength, and said receiving light path is divided into scattered light receiving light path and fluorescence receiving light path, also contains a sampling gas circuit;
＜2〉optical axis (OO of scattered light receiving light path
2) with the optical axis (OO of fluorescence receiving light path
3) on same straight line, and with the optical axis (O of Ultra-Violet Laser illumination path
1O ') is vertically intersected on the central point (O) of photosensitive area (5), the central axis (O of sampling gas circuit
4O ") passes the central point (O) of photosensitive area (5), and perpendicular to the optical axis (O by the Ultra-Violet Laser illumination path
1O '), the optical axis (OO of scattered light receiving light path
2) and the optical axis (OO of fluorescence receiving light path
3) plane that constituted;
＜3〉on the Ultra-Violet Laser illumination path, along on ultraviolet laser (8) the emission light beam progressive direction, by ultraviolet laser (8) between the light trapping (7), same successively optical axis (O
1O ') being equipped with beam expanding lens group (9), cylindrical mirror (10) and light trapping (7), is photosensitive area (5) between cylindrical mirror (10) and light trapping (7), and the central point (O) of photosensitive area (5) is on the focus (O) of cylindrical mirror (10);
＜4〉on the scattered light receiving light path, between photosensitive area (5) and scattered light receptor (13), same optical axis (OO
2) be equipped with the scattered light that constitutes by first collimating mirror (111), scattered light spectral filter (112) and first condensing lens (113) and receive mirror group (11) and scattered light diaphragm (12), wherein the front focus (O) of first collimating mirror (111) overlaps with the central point (O) of photosensitive area (5), and scattered light diaphragm (12) places the back focus (O of first condensing lens (113)
2) on, the output of scattered light receptor (13) links to each other with computer (21) through scattered light amplifier (14);
＜5〉on the fluorescence receiving light path, in photosensitive area (5) between the fluorescence receptor (17), same optical axis (OO
3) be equipped with the fluorescence that constitutes by second collimating mirror (151), fluorescent optical filter (152) and second condensing lens (153) and receive mirror group (15) and fluorescence diaphragm (16), wherein the front focus (O) of second collimating mirror (151) overlaps with the central point (O) of photosensitive area (5), and fluorescence diaphragm (16) places the back focus (O of second condensing lens (153)
3) on, behind the output process fluorescence amplifier (18) of fluorescence receptor (17), link to each other with computer (21) with photon counter (20) by peak-holding circuit (19) respectively;
＜6〉on the sampling gas circuit, contain and place relatively both sides, photosensitive area (5), central axis (O
4O ") passes the inlet pipe (22) and the vapor pipe (23) of photosensitive area (5) central point (O).
2. ultraviolet laser biomone counter according to claim 1 is characterized in that saidly placing the beam expanding lens group (9) on the Ultra-Violet Laser illumination path to be made up of the recessed cylindrical mirror (901) of a short focal length and the protruding cylindrical mirror (902) of a long-focus.
3, ultraviolet laser biomone counter according to claim 1, it is characterized in that said scattered light spectral filter (112) is a kind of arrowband type bandpass filter, the centre wavelength that sees through is positioned at the wavelength place of ultraviolet laser (8), ultraviolet laser (8) emitted laser bundle is seen through, and be total reflection fluorescence.
4, ultraviolet laser biomone counter according to claim 1, it is characterized in that said fluorescent optical filter (152) is a kind of long-pass type spectral filter, fluorescence is seen through, and is total reflection to the UV laser beam of launching less than the ultraviolet laser (8) of wavelength of fluorescence.
5, ultraviolet laser biomone counter according to claim 1 is characterized in that said cylindrical mirror (10) is protruding cylindrical mirror.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02136858.9A CN1219073C (en) | 2002-09-06 | 2002-09-06 | Ultraviolet laser biological particle counter |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02136858.9A CN1219073C (en) | 2002-09-06 | 2002-09-06 | Ultraviolet laser biological particle counter |
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| CN1401788A CN1401788A (en) | 2003-03-12 |
| CN1219073C true CN1219073C (en) | 2005-09-14 |
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|---|---|---|---|
| CN02136858.9A Expired - Lifetime CN1219073C (en) | 2002-09-06 | 2002-09-06 | Ultraviolet laser biological particle counter |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100567788B1 (en) * | 2004-02-13 | 2006-04-05 | 주식회사 현대교정인증기술원 | Apparatus for Measuring Numbers of Particles and Method Thereof |
| CN102297824B (en) * | 2011-05-18 | 2013-05-08 | 北京汇丰隆经济技术开发有限公司 | Single light source bio-aerosol particle detecting apparatus |
| CN107783418A (en) * | 2016-08-25 | 2018-03-09 | 大连楼兰科技股份有限公司 | Automobile fuel consumption adaptive control system and method based on fine particle light scattering |
| CN107515209A (en) * | 2017-10-02 | 2017-12-26 | 西南石油大学 | A kind of Multifunction fluorescent sample lights testboard |
| US10267723B1 (en) * | 2018-01-23 | 2019-04-23 | Cbrn International, Ltd. | Bioaerosol particle detector |
| CN108375530A (en) * | 2018-03-28 | 2018-08-07 | 南京工业大学 | A kind of bioaerosol real-time detection method and device based on laser induced fluorescence |
-
2002
- 2002-09-06 CN CN02136858.9A patent/CN1219073C/en not_active Expired - Lifetime
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