CN1218751C - Two-element active antibody preparation and its preparing process - Google Patents
Two-element active antibody preparation and its preparing process Download PDFInfo
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- CN1218751C CN1218751C CN 00123712 CN00123712A CN1218751C CN 1218751 C CN1218751 C CN 1218751C CN 00123712 CN00123712 CN 00123712 CN 00123712 A CN00123712 A CN 00123712A CN 1218751 C CN1218751 C CN 1218751C
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Abstract
The present invention relates to a dyadic active preparation and a preparation method thereof. The preparation organically combines probiotic bacteria and specific immune globulin, the synergistic effect of the probiotic bacteria and the specific immune globulin is sufficiently exerted to realize the action of various infectious disease prevention and treatment, the disease incidence and the mortality of young animals and young pets are reduced, and the present situation of the growth and development of the young animals and the young pets is improved. The preparation adopts a spray drying method to prepare a yolk antibody and is mixed with various kinds of probiotic bacterium drying powder, so the present invention not only maintains the titer of antibody powder but also effectively solves the preservation and transportation problems of mixed products of the antibody and the probiotic bacteria. The preparation has the characteristics of no pollution, no drug resistance, no residues, long drug effect duration time, low production cost, etc.
Description
The present invention is immunoglobulin, the probiotic bacteria of effective dose and the composition and method of making the same that can accept adjuvant.
Control humans and animals infection aspect antibiotic has been brought into play effect of crucial importance, and but then, its extensive application has brought problems such as Drug resistance, drug residue, environmental pollution.Antibiotic can not only be killed pathogen, also can kill the normal microorganism species useful to body simultaneously, causes the flora disorder, cause secondary infection and autogenous infection, the life-time service antibiotic produces drug resistance, and body's immunity is descended, and therapeutic effect is not good or cause death.Antibiotic application that even more serious is also may cause between the pathogen of humans and animals Drug resistance transmission and drug residue problem take place, and human health and natural environment are caused very big threat.Become the important restraining factors that influence China's livestock products outlet in the caused livestock products safety problem of China's drug residue, caused enormous economic loss.Antibiotic use is subjected to more and more being restricted in various countries.
Constantly explore in other effective ways that improve the humans and animals resistance against diseases people, the research of probiotic bacteria be applied to obtain in recent years bigger development.All there are a large amount of microorganism species in the digestive tract of humans and animals, urogenital tract, respiratory tract and body surface.Normal bacterium has the pathogenic microorganism of inhibition and grows, keeps effects such as intestinal microbial population balance, adjusting gastrointestinal function, raising body non-specific immunity.More than 20 kind of microorganism such as state such as the U.S., Japan approved lactobacillus, bacillus bifidus, bacillus cereus, Bacillus licheniformis, butanoic acid bacillus cereus can be used as the health promoting product of animal, and domestic also have above-mentioned microbial ecological agent product at present.But because directly kill harmful microorganism of microbial ecological agent is therefore usually not ideal enough to the therapeutic effect of actute infection.
Recent study finds, behind the hen that lays eggs with antigen immune, can produce corresponding antibody and transferable and accumulate in yolk in its body, and this antibody is called as Yolk immunoglobulin (Immunoglubulin of the Yolk is called for short IgY).Compare with the mammal IgG antibody, IgY has that output is big, homogeneity good, do not activate the mammal complement system, not the non-specific binding rheumatoid factor, not fixedly staphylococcal protein A, with between the mammalian immune globulin advantage such as serotype reaction do not take place to intersect.Developed at present the product that is used to prevent and treat some disease of humans and animals successively.But because the mechanism of action of IgY is the passivity immunity, so the effect phase is shorter.Prepare the antibody powder owing to generally adopt freeze drying process to purify at present, the production cost height, and preserve and transportation inconvenience, therefore limited its application.
The purpose of this invention is to provide a kind of two-element active preparation and preparation method thereof, said preparation is the organic assembling of probiotic bacteria and specific immune globulin.Its mechanism is to utilize common harmful pathogen in the immunoglobulin specific killing body, reduces infection chance; Utilize probiotic bacteria to adjust the balance of intravital normal microorganism species simultaneously, improve the non-specific anti-infection ability of body; The cooperative effect of giving full play to the two reduces M ﹠ M to reach the preventive and therapeutic effect at multiple infectious disease, improves the growth promoter present situation.Its effect is better than antibiotic and simple probiotic bacteria series products and simple immunoglobulin.Characteristics such as pollution-free, no Drug resistance that said preparation has, noresidue and duration of efficacy are long, and production cost is low.Make yolk antibody dry powder with spray drying method, kept with the tiring and curative effect of the powder body of the high freeze drying process production of cost, and efficiently solved the storage and transport problem of product.
To achieve these goals, the present invention takes following technical scheme:
Immunoglobulin in the described two-element active preparation is selected from: Chinese People's Anti-Japanese Military and Political College's enterobacteria immunoglobulin, anti-salmonella immunoglobulin, anti-bursal disease (IBD) immunoglobulin, anti-newcastle (ND) immunoglobulin, porcine epidemic diarrhea resisting virus immunity globulin, anti-swine infectious enterogastritis virus immunity globulin, rotavirus resisting immune globulin, anti-parvovirus immunoglobulin, infectivity resistant bronchitis virus immunoglobulin, infectivity resistant laryngitis virus immunity globulin, the multi-joint immunoglobulin of anti-IBD-ND etc.
Probiotic bacteria in the described two-element active preparation comprises: bacillus bifidus, lactobacillus, streptococcus faecalis, bacillus cereus, yeast, if the preparation that is made into comprises one or more probiotic bacteria, can ferment respectively or mixed culture fermentation.
The content of immunoglobulin in preparation in the described two-element active preparation is 1-15%.
The quantity of the probiotic bacteria in the described two-element active preparation is 5,000 ten thousand---500,000,000/gram.
For improving the purification purity of immunoglobulin; Immune globulin activity is not destroyed by high temperature in preparation process, adopt the HPMCP sedimentation method, produce from reinforced immunological chicken group and extract corresponding specific immune globulin (IgY) the egg, and make yolk antibody dry powder through drying process with atomizing.
For making immunoglobulin avoid gastric acid and pepsin, tryptic destruction, add enteric coating material---acrylic resin or hydroxypropylmethyl cellulose phthalate; Protein stabiliser---comprise formaldehyde and sucrose.
The present composition cooperates with corresponding probiotic bacteria by choosing different immunoglobulins, can make food additive, health product, medicine, feed additive with prevention and the following function of diseases of treatment:
By young stock fowl, house pet little son, the infantile diarrhea that pathogen such as escherichia coli, Salmonella cause, chicken bursal disease (IBD), newcastle (ND), infectious bronchitis, the infectiousness laryngitis, duck viral hepatitis, pig parvoviral infects.
The invention will be further described below in conjunction with embodiment, but these embodiment do not limit the present invention.
Embodiment one.
Be used to prevent, treat preparation and the curative effect that escherichia coli cause the two-element active preparation of young stock fowl dysentery
(1), preparation
1. epidemic focus preparation
Choose the Escherichia coli seedling bacterial strain that has specific antigen, by known method prepare inactivated vaccine, check is tired and is preserved.
2. animal immune
Adopt 6 monthly age no-special pathogen (SPF) bird inlays, every in chest muscle injection 1 prepared immunogen set by step.Basis injection twice, 10 days at interval.Booster immunization injection 1 time again after 10 days.Regularly detecting serum and Yolk immunoglobulin tires.
3. immunoglobulin preparation
The immune egg surface sterile is handled, homogenize is handled after removing Ovum Gallus domesticus album, add 5-8 times of volume distilled water diluting, adding content is 5% HPMCP (hydroxypropylmethyl cellulose phthalate) suspension and mixing by the preparation of 80% ethanol, and making its final concentration is 0.25% (V/V), 10-15 ℃ left standstill 18 hours, centrifugal 10 minutes of 1500g collects supernatant, by 0.45 μ m membrane filtration degerming, spray drying is made antibody dry powder, and cryopreservation is stand-by.
4. probiotic powder preparation
1) preparation culture medium, composition comprises: peptone, yeast powder, lactoalbumin hydrolysate, Fructus Lycopersici esculenti juice, glucose, lactose, starch.
2) by known method respectively or Mixed culture bacillus bifidus, lactobacillus, intestinal connect coccus, Bacillus licheniformis, bacillus cereus, yeast.When the control bacterial content is 5-10 hundred million/ml, reclaim wet thallus with continuous centrifuge.
3) add protein stabiliser in above-mentioned wet thallus, the starch adsorption method obtains the one-component dry bacterium powder or mixes dry bacterium powder, and the control bacterial content is 5-15 hundred million/gram.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
425 of newborn piglets are chosen in clinical experiment respectively, and (wherein the conventional medicine matched group is 200,225 of test group), (wherein the conventional medicine matched group is 100 for nascent 200 of lambs, 100 of test group), (wherein the conventional medicine matched group is 100 for nascent 200 of calves, 100 of test group), (wherein the conventional medicine matched group is 50 for 100 of chickling, 50 of test group), (wherein the conventional medicine matched group is 50 for 100 of pups, 50 of test group), test group is oral to be used to prevent and treat the two-element active preparation that escherichia coli cause young stock fowl dysentery, matched group is the conventional medicine group, and two groups of other feedings and managements are with normal.The result shows that test group all is significantly increased than matched group survival rate survival rate and age in days average weight gain in weight, and sickness rate significantly reduces.Test data sees Table 1-5, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 1 two-element active preparation is to the influence of newborn piglet
| Project | Test group | Matched group |
| Age in days head number | 225 | 200 |
| Dysentery head number | 15 | 79 |
| Sickness rate | 6.7 | 39.5 |
| A dead number | 7 | 35 |
| Survival rate (%) | 96.9 | 82.5 |
| Birth weight (kg) | 1.386 | 1.378 |
| 21 ages in days heavy (kg) | 5.46 | 5.06 |
| Than matched group heavy (%) | 7.9 |
Table 2 two-element active preparation is to the influence of nascent lamb
| Project | Test group | Matched group |
| The age in days number of elements | 100 | 100 |
| The dysentery number of elements | 6 | 21 |
| Sickness rate | 6 | 21 |
| Dead number of elements | 4 | 32 |
| Survival rate (%) | 96.0 | 68.0 |
| Birth weight (kg) | 4.19 | 4.17 |
| Two monthly ages heavy (kg) | 17.99 | 16.12 |
| Than matched group heavy (%) | 11.6 |
Table 3 two-element active preparation is to the influence of nascent calf
| Project | Test group | Matched group |
| Age in days head number | 100 | 100 |
| Dysentery head number | 2 | 15 |
| Sickness rate | 2 | 15 |
| A dead number | 5 | 23 |
| Survival rate (%) | 95.0 | 77.0 |
Table 4 two-element active preparation is to the influence of chickling
| Project | Test group | Matched group |
| The age in days number of elements | 50 | 50 |
| The dysentery number of elements | 3 | 10 |
| Sickness rate | 6 | 20 |
| Dead number of elements | 2 | 6 |
| Survival rate (%) | 96 | 88 |
| Birth weight (g) | 34 | 34 |
| Result heavy (g) | 126.7 | 107.5 |
| Than matched group heavy (%) | 17.8 |
Table 5 two-element active preparation is to the influence of pup
| Project | Test group | Matched group |
| The experiment number of elements | 50 | 50 |
| The dysentery number of elements | 2 | 11 |
| Sickness rate | 4 | 22 |
| Dead number of elements | 0 | 7 |
| Survival rate (%) | 100 | 84 |
Embodiment two
Be used to prevent, treat preparation and the curative effect that Salmonella causes young stock fowl diarrheal two-element active preparation
(1), preparation
1. immunogen preparing
Choose the Salmonella seedling bacterial strain that has specific antigen, by known method prepare inactivated vaccine, check is tired and is preserved.
2. animal immune
Carry out animal immune by embodiment one described animal immune method.
3. immunoglobulin preparation
Press embodiment one described preparation method for immunoglobulinlg, produce the anti-salmonella immunoglobulin.
4. probiotic powder preparation
Press embodiment one described probiotic powder preparation method, produce single dry component mycopowder or mix dry bacterium powder.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
200 of piglets are chosen in clinical experiment respectively, and (wherein the conventional medicine matched group is 100,100 of test group), (wherein the conventional medicine matched group is 100 for 200 of lambs, 100 of test group), (wherein the conventional medicine matched group is 100 for 200 of calves, 100 of test group), (wherein the conventional medicine matched group is 100 for 200 of chickling, 100 of test group), growing up, (wherein the conventional medicine matched group is 100 for 200 of chickens, 100 of test group), test group is oral to be used for prevention and to treat Salmonella causing young stock fowl diarrheal two-element active preparation, matched group is the conventional medicine group, and two groups of other feedings and managements are with normal.The result shows that test group contrast composition sickness rate significantly reduces.Test data sees Table 6-10, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 6 two-element active preparation is to the influence of piglet
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 5 | 12 |
| Sickness rate | 5 | 12 |
Table 7 two-element active preparation is to the influence of lamb
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 4 | 12 |
| Sickness rate | 4 | 12 |
Table 8 two-element active preparation is to the influence of calf
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 6 | 11 |
| Sickness rate | 6 | 11 |
Table 9 two-element active preparation is to the influence of chickling
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 7 | 16 |
| Sickness rate | 7 | 16 |
The influence of the adult chicken of table 10 two-element active preparation
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| Morbidity is a number only | 5 | 11 |
| Sickness rate | 5 | 11 |
Embodiment three
Prevention, treatment newcastle disease, fabricius bursa disease, infectiousness laryngitis, the infectious bronchitis preparation and the curative effect of two-element active preparation
(1), preparation
1. immunogen preparing
Adopt NDV La Sota Strain, bursal disease virus CJ801 strain, press known method preparation preparation Emulsion deactivation connection Seedling, check is tired and is preserved; Adopt avian infectious laryngitis virus, infectious bronchitis virus seedling Strain respectively, by known method prepare inactivated vaccine, check is tired and is preserved.
2. animal immune
Carry out animal immune by embodiment one described animal immune method.
3. immunoglobulin preparation
Press embodiment one described preparation method for immunoglobulinlg, produce the anti-salmonella immunoglobulin.
4. probiotic powder preparation
Press embodiment one described probiotic powder preparation method, produce single dry component mycopowder or mix dry bacterium powder.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
800 of the adult respectively chickens of clinical experiment, divide four group, 200 every group (wherein the conventional medicine matched group is 100,100 of test group) are respectively applied for the curative effect of test two-element active preparation to newcastle disease, bursal disease, avian infectious laryngitis, infectious bronchitis.The oral two-element active preparation of experimental group, matched group is the conventional medicine group, two groups of other feedings and managements are with normal.The result shows that test group contrast composition sickness rate significantly reduces.Test data sees Table 11-14, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 11 two-element active preparation is to the curative effect of newcastle disease
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 7 | 23 |
| Sickness rate | 7 | 23 |
Table 12 two-element active preparation is to the curative effect of chicken bursal disease
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 5 | 17 |
| Sickness rate | 5 | 17 |
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 3 | 13 |
| Sickness rate | 3 | 13 |
Table 14 two-element active preparation is to the curative effect of infectious bronchitis of chicken
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 7 | 17 |
| Sickness rate | 7 | 17 |
Embodiment four
The piglet disease that prevention, treatment parvovirus, infectiousness gastroenteritis virus, epidemic diarrhea virus the cause preparation and the curative effect of two-element active preparation
(1), preparation
1. immunogen preparing
Adopt piglet parvovirus, infectiousness gastroenteritis virus, epidemic diarrhea virus seedling Strain respectively, by known method prepare inactivated vaccine, check is tired and is preserved.
2. animal immune
Carry out animal immune by embodiment one described animal immune method.
3. immunoglobulin preparation
Press embodiment one described preparation method for immunoglobulinlg, produce the anti-salmonella immunoglobulin.
4. probiotic powder preparation
Press embodiment one described probiotic powder preparation method, produce single dry component mycopowder or mix dry bacterium powder.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
600 of piglets are chosen in clinical experiment respectively, divide three groups, 200 every group (wherein the conventional medicine matched group is 100,100 of test group) are respectively applied for the curative effect of test two-element active preparation to the piglet disease of parvovirus, infectiousness gastroenteritis virus, epidemic diarrhea virus initiation.The oral two-element active preparation of experimental group, matched group is the conventional medicine group, two groups of other feedings and managements are with normal.The result shows that test group contrast composition sickness rate significantly reduces.Test data sees Table 15-17, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 15 two-element active preparation is to the curative effect of the piglet disease of parvovirus initiation
Table 16 two-element active preparation is to the curative effect of the piglet disease of infectiousness gastroenteritis virus initiation
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 7 | 23 |
| Sickness rate | 7 | 23 |
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 7 | 23 |
| Sickness rate | 7 | 23 |
The curative effect of the piglet disease that table 17 two-element active preparation popularity diarrhea virus causes
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 7 | 23 |
| Sickness rate | 7 | 23 |
Embodiment five
Be used to prevent, treat the piglet that rotavirus causes, the preparation and the curative effect of lamb diarrhoea two-element active preparation
(1), preparation
1. immunogen preparing
Choose the former rotavirus seedling Strain that has the specific antigen characteristic, by known method prepare inactivated vaccine, check is tired and is preserved.
2. animal immune
Carry out animal immune by embodiment one described animal immune method.
3. immunoglobulin preparation
Press embodiment one described preparation method for immunoglobulinlg, produce the anti-salmonella immunoglobulin.
4. probiotic powder preparation
Press embodiment one described probiotic powder preparation method, produce single dry component mycopowder or mix dry bacterium powder.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
200 of piglets are chosen in clinical experiment respectively, and (wherein the conventional medicine matched group is 100,100 of test group), 200 of lambs (wherein the conventional medicine matched group is 100,100 of test group), test group is oral to be used to the piglet, the lamb diarrheal two-element active preparation that prevent and treat rotavirus to cause, matched group is the conventional medicine group, and two groups of other feedings and managements are with normal.The result shows that test group contrast composition sickness rate significantly reduces.Test data sees Table 18-19, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 18 two-element active preparation is to the influence of piglet
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 3 | 11 |
| Sickness rate | 3 | 11 |
Table 19 two-element active preparation is to the influence of lamb
| Project | Test group | Matched group |
| An experiment number | 100 | 100 |
| A morbidity number | 5 | 13 |
| Sickness rate | 5 | 13 |
Embodiment six
Prevention, the treatment duck viral hepatitis preparation and the curative effect of two-element active preparation
(1), preparation
1. immunogen preparing
Adopt duck viral hepatitis virus seedling Strain, by known method prepare inactivated vaccine, check is tired and preserve.
2. animal immune
Carry out animal immune by embodiment one described animal immune method.
3. immunoglobulin preparation
Press embodiment one described preparation method for immunoglobulinlg, produce the anti-salmonella immunoglobulin.
4. probiotic powder preparation
Press embodiment one described probiotic powder preparation method, produce single dry component mycopowder or mix dry bacterium powder.
Yolk antibody dry powder with the 20-50% of binary preparation gross mass with above-mentioned one-component dry bacterium powder or mix dry bacterium powder and mix, make enteric dosage form two-element active preparation.
(2), curative effect
200 of the adult respectively ducks of clinical experiment, (wherein the conventional medicine matched group is 100,100 of test group) are used to test the curative effect of two-element active preparation to duck viral hepatitis.The oral two-element active preparation of experimental group, matched group is the conventional medicine group, two groups of other feedings and managements are with normal.The result shows that test group contrast composition sickness rate significantly reduces.Test data sees Table 20, and data are analyzed t check, P＜0.05, significant difference by statistics.
Table 20 two-element active preparation is to the curative effect of duck viral hepatitis
| Project | Test group | Matched group |
| The experiment number of elements | 100 | 100 |
| The morbidity number of elements | 7 | 21 |
| Sickness rate | 7 | 21 |
Listed several embodiment above, wherein data are only for clearly demonstrating the present invention.In fact, described various immunoglobulins of claim 2 and the described several probiotic bacterias of claim 3, can in the scope that pharmacy allows, adjust arbitrarily, might exceed the combination of described immunoglobulin of the embodiment of the invention and probiotic bacteria and proportioning ratio and prepare the two-element active preparation, reach purpose of the present invention.Thereby those skilled in the art, according to the present invention, can change and many embodiments.
Strain used in the present invention is stored in Chinese microorganism strain and contains administration committee agricultural microorganism center (the BaiShiQiao,BeiJing Chinese Academy of Agricultural Sciences, postcode 100081):
1. classification name: Lactobacillus acidophilus bacillus acidophilus, preserve numbering 6006.
2. classification name: Bacillus subtilis (Ehrenberg) Cohn bacillus cereus, preserve numbering ACC11060.
3. classification name: Candiba utilis Henneberg Lodeler et.Kreger Van Rig produces former candidiasis, preserves numbering ACC2059.
4. classification name: Bacillus licheniformis Bacillus licheniformis, preserve numbering 20386.
5. classification name: Streptococcus faecalls streptococcus faecalis, preserve numbering 6049.
6. classification name: Bifidobacteriun bifidum bifidobacterium bifidum, preserve numbering 1-1852.
Claims (12)
1. two-element active preparation: the compositions of chicken yolk immunoglobulin, probiotic bacteria and acceptable auxiliary that this two-element active preparation is an effective dose.
2. two-element active preparation according to claim 1, it is characterized in that, described immunoglobulin is selected from: Chinese People's Anti-Japanese Military and Political College's enterobacteria immunoglobulin, anti-salmonella immunoglobulin, anti-bursal disease IBD immunoglobulin, anti-newcastle ND immunoglobulin, porcine epidemic diarrhea resisting virus immunity globulin, anti-swine infectious enterogastritis virus immunity globulin, albumen, infectivity resistant bronchitis virus immunoglobulin, infectivity resistant laryngitis virus immunity globulin and the multi-joint immunoglobulin of IBD-ND are asked in rotavirus resisting immune globulin, anti-parvovirus immunity.
3. two-element active preparation according to claim 1 is characterized in that, described probiotic bacteria is selected from: bacillus bifidus, lactobacillus, streptococcus faecalis, bacillus cereus, yeast, the viable count in compositions are 5,000 ten thousand-500,000,000/gram.
4. two-element active preparation according to claim 1 is characterized in that said composition is the enteric dosage form.
5. two-element active preparation according to claim 1 is used to prevent, treat the purposes of the multiple livestock and poultry that is caused by pathogen such as antibacterial, viruses.
6. according to claim 1 or 4 described two-element active preparations, it is characterized in that said composition can be made into microcapsule, unguentum, electuary.
7. two-element active preparation according to claim 6 is characterized in that described adjuvant is selected from enteric coating material, protein stabiliser.
8. according to claim 1 or 6 described two-element active preparations, it is characterized in that immunoglobulin content wherein is the 1--15% of composition quality.
9. two-element active preparation according to claim 7 is characterized in that described enteric coating material is an acrylic resin, hydroxypropylmethyl cellulose phthalate, hydroxypropyl emthylcellulose.
10. two-element active preparation according to claim 7 is characterized in that described protein stabiliser is formaldehyde, sucrose.
11. two-element active preparation according to claim 1 is characterized in that: said preparation is as feed additive.
12. a production technology for preparing the described two-element active preparation of claim 1 is characterized in that this two-element active preparation is to be prepared from through following steps:
1) carries out reinforced immunological with no-special pathogen SPF chicken;
2) adopt the HPMCP sedimentation method, the egg that reinforced immunological chicken group is produced carries out the surface sterile processing, homogenize is handled after removing Ovum Gallus domesticus album, adds 5-8 times of volume distilled water diluting, and adding content is 5% HPMCP suspension and mixing by the preparation of 80% ethanol, making its final concentration is 0.25%, 10-15 ℃ left standstill 18 hours, and centrifugal 10 minutes of 1500g collects supernatant, by 0.45 μ m membrane filtration degerming, spray drying is made antibody dry powder.
3) adopt conventional culture medium culturing bacillus bifidus, lactobacillus, intestinal to connect coccus, Bacillus licheniformis, bacillus cereus, yeast, when the control bacterial content is 5-10 hundred million/ml, reclaim wet thallus with continuous centrifuge, in above-mentioned wet thallus, add protein stabiliser, the starch adsorption method obtains the one-component dry bacterium powder, and the control bacterial content is 5-15 hundred million/gram.
4) immunoglobulin content according to claim 8, the 20-50% with binary preparation gross mass mixes with dry bacterium powder yolk antibody dry powder, makes enteric dosage form two-element active preparation at last.
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| BR112013012594A2 (en) * | 2010-11-23 | 2018-10-16 | Pantheryx Inc | compositions and methods for treatment in broad spectrum undifferentiated or mixed clinical applications |
| CN104548093A (en) * | 2014-12-22 | 2015-04-29 | 天津瑞普生物技术股份有限公司 | Composition for treating piglet diarrhea and preparation method of composition |
| CN106310251A (en) * | 2016-08-23 | 2017-01-11 | 河北中贝佳美生物科技有限公司 | Anti-virus and IgY antibody improving chicken formula and preparing method thereof |
| CN111345473A (en) * | 2020-03-13 | 2020-06-30 | 珠海华敏医药科技有限公司 | Probiotics composition containing yolk antibody IgY and application preparation |
| CN113198014A (en) * | 2021-03-25 | 2021-08-03 | 河南省健达动保有限公司 | Probiotic fermented traditional Chinese medicine compound immunoglobulin preparation and preparation method thereof |
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