CN1212328C - Preparation method of fucosan sulphate oligosaccharide - Google Patents
Preparation method of fucosan sulphate oligosaccharide Download PDFInfo
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- CN1212328C CN1212328C CN 03111833 CN03111833A CN1212328C CN 1212328 C CN1212328 C CN 1212328C CN 03111833 CN03111833 CN 03111833 CN 03111833 A CN03111833 A CN 03111833A CN 1212328 C CN1212328 C CN 1212328C
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- fucosan
- sulphate
- oligosaccharide
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- fucoidan
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Abstract
The present invention relates to a method for preparing fucosan sulphate oligosaccharide. The present invention is characterized in that fucosan sulphate oligosaccharide is prepared into a water solution; oxidizing agents are added; an obtained mixed solution is heated; a membrane with catch molecular weight of 3000 to 5000 is used for ultrafiltration; ultrafiltrate is treated by vacuum concentration and freeze-drying, wherein the oxidizing agents are hydrogen peroxide or hypochlorous acid and the salt thereof or nitrous acid and the salt thereof; the weight ratio of fucosan sulphate to the oxidizing agents is 1:1 to 3; the present invention has the advantages of a few procedures, easy operation, low cost, etc. and is suitable for industrial production. Prepared fucosan sulphate oligosaccharide has significant protective action on a mouse with hepatic injury caused by carbon tetrachloride, D-galactosamine and lipopolysaccharide; the fucosan sulphate oligosaccharide can obviously reduce the activity of serum lutamic-pyruvic transaminase in blood serum and the content of lipid peroxide, enhance the activity of superoxide dismutase(SOD) and glutathione peroxidase, and has the function of protecting the liver; fucosan sulphate oligosaccharide can be used as a raw material for making marine drugs and health foods.
Description
Technical field
The present invention relates to a kind of preparation method with fucoidan oligosaccharide of liver protecting function.
Background technology
Fucoidan (Fucoidan) is the water-soluble sulfated polysaccharide of a class in the brown alga, and it mainly consists of Fucose and organo-sulfate.Studies show that in a large number that fucoidan has anticoagulation, improves immunizing power, antitumor, antiviral, hypoglycemic, anti-inflammatory, radioprotective, inhibition ascites carcinoma isoreactivity, but in traditional brown alga processing and utilization fucoidan as brown alga in an important activity composition, be not fully used.And fucoidan oligosaccharide report seldom, and mentioning it in the report has functions such as antiviral, anticoagulation.The preparation method of fucoidan oligosaccharide also only limits to the acid degradation method.The reaction conditions of this method is violent, difficult operation, and need complicated desalination step, be not suitable for suitability for industrialized production.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of fucoidan oligosaccharide, it can overcome the above-mentioned shortcoming of existing method.
A kind of preparation method of fucoidan oligosaccharide is characterized in that fucoidan is mixed with the aqueous solution, adds oxygenant, and the gained mixing solutions is heated; Membrane ultrafiltration with molecular weight cut-off 3000-5000; With the outer liquid vacuum concentration postlyophilization of ultrafiltration.
The present invention's oxidative degradation, and the temperature and time of control degradation, with the ultra-filtration membrane of different molecular weight cut-offs, the fucoidan oligosaccharide of preparation different molecular weight, it is few to have step, operation easily, low cost and other advantages is suitable for suitability for industrialized production.The fucoidan oligosaccharide of preparation has stronger provide protection to the liver injury mouse that is caused by tetracol phenixin, D-Gal and lipopolysaccharides; can significantly reduce the content of the activity and the lipid peroxide (MDA) of serum glutamic pyruvic transminase in the serum (GPT); improve the activity of superoxide dismutase (SOD) and Selenoperoxidase (GSH-Px), have the function of liver protecting.Also can be used as the raw material of marine drug or protective foods.
Embodiment
Below by embodiment the present invention is described.
The fucoidan crude product 50g that will extract from sea-tangle is dissolved in the water of 2000mL, adds the aqueous hydrogen peroxide solution 250ml of 30% (concentration expressed in percentage by weight) in the polysaccharide solution of gained, stirs.The gained mixing solutions is reacted 6h down at 60 ℃.The centrifugal impurity of removing, the supernatant liquor that obtains is crossed the ultra-filtration membrane of molecular weight 3000, and the outer liquid of ultrafiltration through the vacuum concentration postlyophilization, is obtained 32g fucoidan oligosaccharide product.
Used oxidants hydrogen peroxide can be used hypochlorous acid or nitrous acid or their salt instead in the present embodiment, and the weight ratio of described fucoidan and oxygenant is 1: 1～3.Described Heating temperature is 60-120 ℃.The concentration expressed in percentage by weight of fucoidan is 0.5-5 in the described aqueous solution.
Fucoidan used among the present invention can extract from brown algas such as sea-tangle, wakame, sargassun, Sargassum fusiforme.The fucoidan oligosaccharide that makes contain Fucose group 5-35%, organo-sulfate group 5-25%, uronic acid group 1-15%.
Claims (4)
1, a kind of preparation method of fucoidan oligosaccharide is characterized in that fucoidan is mixed with the aqueous solution, adds oxygenant, and the gained mixing solutions is heated; Membrane ultrafiltration with molecular weight cut-off 3000-5000; With the outer liquid vacuum concentration postlyophilization of ultrafiltration.
2, preparation method as claimed in claim 1 is characterized in that: described Heating temperature is 60-120 ℃.
3, preparation method as claimed in claim 1 is characterized in that described oxygenant is hydrogen peroxide or hypochlorous acid and salt or nitrous acid and salt thereof, and the weight ratio of described fucoidan and oxygenant is 1: 1～3.
4, preparation method as claimed in claim 1, the concentration expressed in percentage by weight that it is characterized in that fucoidan in the described aqueous solution is 0.5-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03111833 CN1212328C (en) | 2003-01-15 | 2003-01-15 | Preparation method of fucosan sulphate oligosaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03111833 CN1212328C (en) | 2003-01-15 | 2003-01-15 | Preparation method of fucosan sulphate oligosaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1517356A CN1517356A (en) | 2004-08-04 |
| CN1212328C true CN1212328C (en) | 2005-07-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03111833 Expired - Fee Related CN1212328C (en) | 2003-01-15 | 2003-01-15 | Preparation method of fucosan sulphate oligosaccharide |
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| CN (1) | CN1212328C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2540307B1 (en) * | 2010-02-24 | 2016-05-25 | Morinaga Milk Industry Co., Ltd. | Antibacterial auxiliary agent comprising kombu extract as active ingredient, antibacterial composition, and food or beverage |
| CN101962415A (en) * | 2010-10-28 | 2011-02-02 | 中国海洋大学 | Method for preparing low molecular weight brown seaweed fucoidan sulfate |
| CN102367285B (en) * | 2011-11-06 | 2014-03-05 | 浙江大学 | Pearsonothuria graeffei fucosylated chondroitin sulfate, extracting method thereof, and purpose thereof |
| CN103804504B (en) * | 2014-01-22 | 2015-12-30 | 山东大学（威海） | Low molecule fucoidan and the effect to diabetic nephropathy thereof |
| CN110357983B (en) * | 2019-08-14 | 2021-08-31 | 中国海洋大学 | Preparation method of sea cucumber fucosan sulfate and chondroitin sulfate oligosaccharide |
| CN110693772B (en) * | 2019-11-12 | 2021-11-23 | 烟台新时代健康产业日化有限公司 | Preparation method of seaweed anti-inflammatory extract applied to cosmetics |
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2003
- 2003-01-15 CN CN 03111833 patent/CN1212328C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1517356A (en) | 2004-08-04 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050727 Termination date: 20130115 |