CN1208467C - High-germanium ganoderic polysaccharide and ganoderic acid extraction method - Google Patents
High-germanium ganoderic polysaccharide and ganoderic acid extraction method Download PDFInfo
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- CN1208467C CN1208467C CN 01138214 CN01138214A CN1208467C CN 1208467 C CN1208467 C CN 1208467C CN 01138214 CN01138214 CN 01138214 CN 01138214 A CN01138214 A CN 01138214A CN 1208467 C CN1208467 C CN 1208467C
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Abstract
The present invention relates to a method for extracting high germanium ganoderic polysaccharide and ganoderic acid. The present invention solves the defects that because of restriction of technical conditions, the molecular activity of extract is low, the output and the quality are low, etc. existing in the existing strain fermentation preparation method and the existing water extraction technical method. The method mainly adopts sweet wine to soften wild ganoderma, and ganoderic primary pulp is prepared; substances which contain carbon sources, nitrogen sources, inorganic salt, vitamin, etc. are prepared into auxiliary culture media; the ganoderic primary pulp and the auxiliary culture media are mixed, and through fermentation, filtration and extraction in an aerobic mode, high germanium ganoderic polysaccharide and ganoderic acid can be obtained. Through the method, active substances, such as ganoderic polysaccharide, ganoderic acid, organic germanium, etc., which have high yield can be obtain. The method is a preparation method which has the wide industrialization prospect.
Description
Technical field
The present invention relates to a kind of healthcare products extracting method that is absorbed by the body easily, the extracting method of high germanium glossy ganoderma polysaccharide of particularly a kind of molecular activity and Ganodenic acid.
Background technology
Glossy ganoderma is Basidiomycetes, polyporaceae, Ganoderma fungi.Glossy ganoderma is the famous medicinal fungus of China, particularly the Changbai mountain, Jilin glossy ganoderma is enjoyed the laudatory title of " Wan Nianqin; Ganoderma lucidum seu Japonicum ", the Shennong Bencaojing note is planted: " glossy ganoderma can make light of one's life by commiting suicide (fat-reducing), not forget (brain tonic, intelligence development, memory), treatment hypertension, protect the liver (treatment hepatopathy) detoxifcation; for cardiovascular diseases, the multiple disease of diabetes certain curative effect and prophylactic effect are arranged, and the energy cancer-resisting ".What glossy ganoderma is among the people to be had " having eaten Ganoderma lucidum seu Japonicum, alive for evermore " says.Modern medicine study finds that contained effective ingredient mainly contains polyose (being ganoderan), triterpenes (being mainly the glossy ganoderma acids), polypeptide and organic germanium glycosides, sterols, alkaloids, furan derivative, protein etc. in the glossy ganoderma; The medicinal efficacy of finding glossy ganoderma after deliberation mainly is relevant with the pharmacologically active of ganoderan, Ganodenic acid, organic germanium, and pharmacologically active is relevant with their structure.Therefore shortcomings such as existing strain fermentation working system and water extraction process method exist organic germanium content low because the restriction of processing condition causes having killed the part active substance, and molecular activity is low reduce the pharmacological effect of extract greatly.
Summary of the invention
The purpose of this invention is to provide a kind of deficiency of improving other people invention analogous products technology, and with strain fermentation working system and water extraction process method hypotrophy, shortcoming such as organic germanium content is low, and molecular activity is low.And a kind of extract effective ingredient that increases substantially is provided, particularly improve organic germanium, ganoderan, ganoderic acid content, and the method for other glossy ganoderma effective component content of corresponding raising simultaneously.
For achieving the above object, the experiment comparative analysis by a large amount of the invention provides technical scheme as described below:
One, system glossy ganoderma magma: take wild Ganoderma, Changbai mountain, Jilin wild Ganoderma the best, the extracting solution that adds glossy ganoderma triplication sweet wine softens to be handled 2-5 days, and best 3 days, the back made ganoderma particles be about 1-3mm with the pulverizer making beating, is glossy ganoderma magma.Described sweet wine is a sticky rice wine.
Two, system auxiliary material substratum: substratum can adopt the conventional material that contains compositions such as carbon source, nitrogenous source, inorganic salt and VITAMIN.Wherein: carbon source material comprises potato starch, W-Gum, sucrose, maltose, lactose etc.; Nitrogen source comprises soybean cake powder etc.; Inorganic salt comprise potassium primary phosphate, dipotassium hydrogen phosphate, lime powder, positive sodium germanate, fused(calcium magnesium)phosphate etc., and the summation 6-12 moisture content doubly of adding auxiliary material, be preferably the moisture content of 9 times of auxiliary material summations, wherein add positive sodium germanate in the inorganic salt and can be converted into organic germanium by fermentation, transformation efficiency is 100%, thereby reaches the purpose of high germanium.
Three, with described glossy ganoderma magma 1 weight part proportioning auxiliary material substratum 10 weight parts and to regulate its pH value be that 6.5-7 carries out aerobic fermentation, its leavening temperature is 25 ℃-35 ℃, best 28 ℃-32 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus again and stir again, temperature was cultivated 2 days under 28 ℃-35 ℃ condition, stop fermentation, carry out defibrination twice with paste roller mill.
Four, described cultured defibrination is carried out three roads and filter, first road filters and adopts 30 orders to filter, and second road filters and adopts 60 orders to filter, and the 3rd road filters and adopts the 80-100 order to filter, more than three roads filter and all adopt disposable mechanical shock formula filtration method; With the stoste after filtering, add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, back spraying type drying treatment, spraying drying wind-force is the 4-5 level, and 38 ℃ of-40 ℃ of sterilisable chambers of temperature are operated, and the water content of finally taking a sample is till 12%, packs again.
The present invention has following advantage: because the present invention adopts pure natural Changbai mountain, Jilin glossy ganoderma to add pure microbial fermentation and processing, through experimental analysis proof and other similarly invention as by water-boiling method with utilize the strain fermentation method to compare its molecular activity to have improved 500-800%, herein, germanic amount has improved 2-3 doubly, contain Ganodenic acid and ganoderan and improved 2-3 doubly, the also corresponding raising of other content of active substance.Glossy ganoderma utilization ratio and commodity value have been improved widely, present method is easy owing to not increasing complicated optional equipment and culture technique in addition, as in industrial realization Ganodenic acid, ganoderan, these several high value effective constituents of organic germanium, producing, the economic benefit of production process will be increased substantially, also considerable social benefit can be brought.These products all have higher pharmaceutical use, can be widely used in industries such as medicine and health care of food.
Embodiment
Specifically describe performance of the present invention below in conjunction with embodiment:
Embodiment one:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 degree, soften and handled 2 days, making beating, add 6 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out aerobic fermentation, its leavening temperature is 25 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture (temperature is the same) again and stopped fermentation in two days again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has maltose 38 grams per liters, soybean cake powder 8 grams per liters, positive sodium germanate 1.5 grams per liters, dipotassium hydrogen phosphate 0.5 grams per liter, VB0.06 grams per liter in the described fermentation broth.
Analytical results: ganoderan, Ganodenic acid output are respectively 3.76 grams per liters, and 6.2 milligrams/100 milligrams, organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment two:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 degree softens to be handled 5 days, making beating, add 12 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out aerobic fermentation, its leavening temperature is 35 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture two days (temperature is the same) again and stop fermentation again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has potato starch 42 grams per liters, soybean cake powder 9 grams per liters, positive sodium germanate 1.8 grams per liters, potassium primary phosphate 0.4 grams per liter, VB0.07 grams per liter in the described fermented liquid substratum.
Analytical results: ganoderan, Ganodenic acid output are respectively 3.63 grams per liters, and 6.1 milligrams/100 milligrams, organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment three:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 degree softens to be handled 3 days, making beating, add 9 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out the aerobic fermentation method, its leavening temperature is 28 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture two days (temperature is the same) again and stop fermentation again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has W-Gum 41 grams per liters, soybean cake powder 6 grams per liters, positive sodium germanate 1.6 grams per liters, lime powder 0.4 grams per liter, VB0.05 grams per liter in the described fermentation broth.
Analytical results: ganoderan, Ganodenic acid output are respectively 4.94 grams per liters, and 6.9 milligrams/100 milligrams, organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment four:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 ℃ is softened processing 4 days, making beating, add 10 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out the aerobic fermentation method, its leavening temperature is 32 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture (temperature is the same) again and stopped fermentation in two days again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has sucrose 45 grams per liters, soybean cake powder 7 grams per liters, positive sodium germanate 2.0 grams per liters, dipotassium hydrogen phosphate 0.4 grams per liter, VB0.08 grams per liter in the described fermentation broth.
Analytical results: ganoderan, Ganodenic acid output are respectively 4.36 grams per liters, and 6.1 milligrams/100 milligrams, organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment five:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 degree softens to be handled 3 days, making beating, add 8 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out the aerobic fermentation method, its leavening temperature is 30 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture (temperature is the same) again and stopped fermentation in two days again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has lactose 43 grams per liters, soybean cake powder 8 grams per liters, positive sodium germanate 2.2 grams per liters, U.S. phosphate fertilizer 0.5 grams per liter of calcium, VB0.09 grams per liter in the described fermentation broth.
Analytical results: ganoderan, Ganodenic acid output are respectively 4.46 grams per liters, and 6.5 milligrams/100 milligrams, organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment six:
Select for use Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) to add the triple sweet wine, alcoholic strength 8-10 ℃ is softened processing 3 days, making beating, add 11 times of auxiliary material culture medium culturing, and to regulate its pH value be that 6.5-7 carries out the aerobic fermentation method, its leavening temperature is 29 ℃, and adopt the stirring-type (rotating speed 120-160 rev/min) that circles round to ferment 8-10 days, thereafter leave standstill cultivation (under 28 ℃-35 ℃ condition) 10-15 days again, add Bacillus subtilus stir culture (temperature is the same) again and stopped fermentation in two days again, carry out defibrination twice with paste roller mill then, filter through three roads again, and add 1/1000 Streptomycin sulphate and carry out static cultivation 5-7 days, stoste is carried out the spraying type drying treatment, promptly get high germanium glossy ganoderma polysaccharide of molecular activity and Ganodenic acid.
Content has maltose 36 grams per liters, soybean cake powder 10 grams per liters, positive sodium germanate 2.5 grams per liters, dipotassium hydrogen phosphate 0.7 grams per liter, VB0.1 grams per liter, Bacillus subtilus 2 grams per liters in the described fermentation broth.
Bacillus subtilus is added in the auxiliary material substratum simultaneously, and Bacillus subtilus should use safety as recipient cell, can directly separate from fermented liquid and get active protein, utilizes recombinant DNA technology can improve the output and the quality of tunning effectively.Molecular activity is more effectively improved.
Analytical results, ganoderan, Ganodenic acid output are respectively 5.12 grams per liters, 6.3 milligrams/100 milligrams, and organic germanium content improves a lot, and other active ingredient also has corresponding raising.
Embodiment seven: the natural glossy ganoderma of Changbai mountain, Jilin natural glossy ganoderma (more than 10 years) or its southern area and country (more than 20 years) or artificial linden wood cultivation can be adopted and bury the glossy ganoderma that indigenous method produces and directly soaked 5 days by sweet wine (8-10 ° alcohol amount), wherein add 2/1000 positive sodium germanate, Bacillus subtilus, soak pulled an oar again in 5 days, filtration, spray-drying process, promptly get similar products like, certain this method cost is than embodiment one, embodiment two high several times, but advantage be produce very simple.But its effective ingredient of glossy ganoderma of artificial linden wood cultivation has only the function of the 30-40% of embodiment one, embodiment two, and its substituting stuff cultivation is more poor, only is equivalent to utilize the product of strain fermentation.
More than selected only be preferential embodiment of the present invention; need to prove: for one skilled in the art; under the prerequisite of inventive principle, can also make some improvement and distortion to the present invention, this belongs to protection scope of the present invention equally.
Claims (6)
1, a kind of high germanium glossy ganoderma polysaccharide and Ganodenic acid extracting method is characterized in that it comprises following operation:
(1) system glossy ganoderma magma: take wild Ganoderma, add the softening processing of sweet wine 2～5 days, making beating then;
(2) system auxiliary material substratum: as the auxiliary material substratum, the moisture content that adds 6～12 times of auxiliary material total amounts mixes with the material that contains carbon source, nitrogenous source, positive sodium germanate, dipotassium hydrogen phosphate and VITAMIN composition;
(3) glossy ganoderma magma 1 weight part and auxiliary material substratum 10 weight parts were carried out aerobic fermentation 8～10 days, leave standstill again and cultivated 10～15 days, adding fermenting bacillus subtilis defibrination after 2 days;
(4) basic three roads of described slurry are filtered, spraying drying gets final product.
2,, it is characterized in that the softening time of handling is 3 days in the described system glossy ganoderma magma step according to the extracting method of described a kind of high germanium glossy ganoderma polysaccharide of claim 1 and Ganodenic acid.
3,, it is characterized in that adding moisture content in the described system auxiliary material substratum is 9 times of weight parts of auxiliary material summation according to the extracting method of described a kind of high germanium glossy ganoderma polysaccharide of claim 1 and Ganodenic acid.
4, according to the extracting method of described a kind of high germanium glossy ganoderma polysaccharide of claim 1 and Ganodenic acid, it is characterized in that the pH of mixed value is 6.5～7 in the described aerobic fermentation process, it is 25～35 ℃ at first at leavening temperature, fermented 8～10 days, and added Bacillus subtilus again in 10～15 days and under 28～35 ℃ of conditions, cultivated 2 days leaving standstill under 28～35 ℃ of conditions to cultivate again.
5, according to the extracting method of described a kind of high germanium glossy ganoderma polysaccharide of claim 1 and Ganodenic acid, it is characterized in that the filtration of described three roads is meant that first road filters employing 30 orders and filters, second road filters and adopts 60 orders to filter, the 3rd road filters and adopts 80～100 orders to filter, and the filtration of described three roads all adopts disposable mechanical shock formula filtration method to filter.
6, according to the extracting method of claim 1 or 4 described a kind of high germanium glossy ganoderma polysaccharide and Ganodenic acid, it is characterized in that containing in the described substratum maltose 36 grams per liters, soybean cake powder 10 grams per liters, positive sodium germanate 2.5 grams per liters, dipotassium hydrogen phosphate 0.7 grams per liter, vitamins B 0.1 grams per liter, Bacillus subtilus 2 grams per liters.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 01138214 CN1208467C (en) | 2001-11-26 | 2001-11-26 | High-germanium ganoderic polysaccharide and ganoderic acid extraction method |
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| CN 01138214 CN1208467C (en) | 2001-11-26 | 2001-11-26 | High-germanium ganoderic polysaccharide and ganoderic acid extraction method |
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| CN1208467C true CN1208467C (en) | 2005-06-29 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100335646C (en) * | 2003-10-23 | 2007-09-05 | 傅世桥 | Comprehensive utilization production technology of high content of glossg ganoderma polysaccharide refined powder and its by product |
| CN1297569C (en) * | 2004-10-20 | 2007-01-31 | 上海市农业科学院 | Preparation method of ganoderma polysaccharide |
| CN107916230A (en) * | 2017-12-01 | 2018-04-17 | 中国农业科学院麻类研究所 | A kind of Ganoderma lucidum mycelium zymotic fluid of high ganoderma polyoses content and preparation method thereof |
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