CN1204651A - Preparation method for spirulina polypeptide and medicament containing these polypeptide and application - Google Patents
Preparation method for spirulina polypeptide and medicament containing these polypeptide and application Download PDFInfo
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- CN1204651A CN1204651A CN 98102406 CN98102406A CN1204651A CN 1204651 A CN1204651 A CN 1204651A CN 98102406 CN98102406 CN 98102406 CN 98102406 A CN98102406 A CN 98102406A CN 1204651 A CN1204651 A CN 1204651A
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Abstract
A spirulina polypeptide having more physiologic activities is obtained by hydrolyzing the spirulina protein with hydrolytic protease obtained from animal, plant, microbe and gene recombined microbe in the condition of acidic, alkaline, or neutral environment or existance of Mg, Ca, P, Se, Zn and Mn ions. Said spirulina polypeptide or its acid salt or its compound can be used as active component in medicine or health-care products.
Description
The present invention relates to the preparation method of spirulina polypeptide and preparation thereof and use, more particularly, the present invention relates to have its acid salt and the mixture thereof of the spirulina polypeptide of multiple physiologically active.The invention still further relates to and contain at least a or multiple spirulina polypeptide: its acid salt or its mixture maybe can be done medicinal and purposes healthcare products as activeconstituents.
High-load phycoerythrin, the blue albumen of algae are arranged in spirulina, become blue albumen of algae and high-content collagen protein.Be generically and collectively referred to as algae albumen.
Because these albumen are peptide compounds, therefore decompose at digestive tube usually, and because they are macromolecule state of aggregation compounds.Therefore also be difficult to fully be absorbed from digestion organs.
The algae albumen that purpose of the present invention just provides spirulina carries out chemistry and biological enzymolysis, prepares the multiple little peptide that has more various physiologically actives than original algae albumen.This physiologically active peptide claims functional peptide again, has physiological regulatory action.
The multiple little peptide that another object of the present invention just provides phycobiliprotein has hypotensive, decreasing cholesterol, and reducing blood-fat, antitumor, the preventing cardiovascular disease effect has hypoglycemicly simultaneously, prevents multiple physiological active functionses such as obesity.
Three of purpose of the present invention just provides a kind of medicinal compositions, and it contains at least a or multiple little peptide as activeconstituents.
The inventor has reached the multiple little peptide of above purpose of the present invention through further investigation and has had multiple physiological activity, to satisfy the requirement as functional health care food or medicine.
Cholesterol is that necessary physiologically substance can be converted into bile acide, steroid hormone and vitamins D in vivo in the body except that the composition as cytolemma
3But hypercholesterolia, savings can make lumen of vessels narrow down on vessel wall, and blood flow, needs after the effect of enzyme after the cholesterol in the food enters in the body by being obstructed, and becoming the solid acid esters of courage could be in intestinal absorption, and this process need bile acide participates in.Bile acide is a surface-active substance, and it has emulsifying effect to lipid.The active group of the little peptide of the present invention is easy to the bile acide combination and excretes, because the bile acide around the cholesterol disappears, this kind of enzyme just can't make cholesterol be transformed into easily by the cholesterol of intestinal absorption.In order to keep the normal physiological content of bile acide, human body just must convert cholesterol to bile acide in liver so, and the cholesterol level in the blood must be descended, and plays lipopenicillinase, the decreasing cholesterol effect.
The principle of reatment of essential hypertension is the picked-up of restriction salt, and can make has patient's blood pressure drops of 60% approximately.Clinical trial in recent years proves, elevation of blood pressure is only relevant with the chlorion in the salt, and it is irrelevant with sodium ion, chlorion in the salt can make the Zinc metallopeptidase Zace1 activation, this enzyme catalysis angiotensin I changes Angiotensin II into, and Angiotensin II can promote the aldosterone secretion, makes to keep sodium and water in the body, so that Q volume of blood increases, elevation of blood pressure.The proteic little Toplink of algae of the present invention acts on mutually with chlorion, and drains jointly in ight soil, make to lack chlorion in the body, and the Zinc metallopeptidase Zace1 non-activity, Angiotensin II reduces, thereby blood pressure is no longer raise, and plays the adjusting blood vessel function.
Algae albumen is through chemistry and enzymatic treatment, makes in the molecule of multiple little peptide and is rich in amino, has alkalescence.With the reaction of hydrochloric acid in gastric juice in can generate ammonium salt, can make pH alkalitropism side displacement in the intestines, improve acidic constitution.Because the Trypsin deficiency can cause diabetes, it is acid that its patient's body fluid is.Because the little peptide of this spirulina to slightly acidic, just can improve the utilization ratio of Regular Insulin to pH regulator, helps the control of diabetes.In addition, it also has the function of endocrine regulation, makes insulin secretion normal, suppresses blood sugar and rises.The little peptide of this spirulina also can be discharged unnecessary sugar in the body, prevents obesity, helps preventing diabetes.
(the β is subunit of 6 γ forms are for Phycoerythrin in the algae albumen (R-phycoerythrin) has that a); And its most stable state of aggregation molecular weight is 263; 000.Blue (β is 6 subunits forms are for albumen of its algae (C-phycocyanin) has that a); And this most stable state of aggregation molecular weight is 224; 000.It (β is 3 subunit forms are for becomes blue albumen of algae (allophycicyanin) has that a); And its most stable state of aggregation molecular weight is 104; 000.a162： 10-Mel-Lys-Thr-Pro-Ile-Thr-Glu-Ala-Ile-Ala-Ala-Ala 20-Asp-Asn-Gln-Gly-Arg-Phe-Leu-Ser-Asn-Thr-Glu-Leu 30-Gln-Ala-Val-Asn-Gjy-Arg-Tyr-Gln-Arg-Ala-Ala-Ala 40-Ser-Leu-Glu-Ala-Ala-Arg-Ser-Leu-Thr-Ser-Asn-Ala 50 60-Glu-Arg-Leu-Ile-Asn-Gly-Ala-Ala-Gln-Ala-Val-Tyr 70-Ser-Lys-Phe-Pro-Tyr-Thy-Ser-Gln-Met-Pro-Gly-pro 80-Gln-Tyr-Ala-Ser-Ser-Ala-Val-Gly-Lys-Ala-Lys-Cys 90-Ala-Arg-Asp-Ile-Gly-Tyr-Tyr-Leu-Arg-Met-Vai-Thr 100-Thr-Cys-Leu-Val-Val-Gly-Gly-Thr-Gly-Pro-Met-Asp 110 120-Gly-Tyr-Leu-Ile-Ala-Gly-Leu-Gly-Gly-Ile-Asn-Arg 130-Thr-Phe-Asp-Leu-Ser-Pro-Ser-Trp-Tyr-Val-Glu-Ala 140-Leu-Asn-Tyr-Ile-Lys-Ala-Asn-His-Gly-Len-Ser-Gly 150-Gln-Ala-Ala-Asn-Glu-Ala-Asn-Thr-Tyr-Ile-Asp-Tyr 160-Ala-Ile-Asn-Ala-Leu-Serβ172： 10-Met-Leu-Asp-Ala-Phe-Ala-Lys-Val-Val-Ala-Gln-Ala 20-Asp-Ala-Arg-Gly-Glu-Phe-Leu-Ser-Asn-Thr-Gln-Leu 30-Asp-Ala-Leu-Ser-Lys-Met-Val-Ser-Glu-Gly-Asn-Lys 40-Arg-Leu-Asp-Val-Val-Asn-Arg-Ile-Thr-Ser-Asn-Ala 50 60-Ser-Ala-Ile-Val-Thr-Asn-Ala-Ala-Arg-Ala-Leu-Phe 70-Ser-Glu-Glu-Pro-Gln-Leu-Ile-Gln-Pro-Gly-Gly-Thr 80-Ala-Tyr-Thr-Asn-Arg-Arg-Met-Ala-Ala-Cys-Len-Arg 90-Asp-Met-Glu-Ile-Ile-Leu-Arg-Tyr-Val-Ser-Tyr-Ala 100-Ile-Ile-Ala-Gly-Asp-Ser-Ser-Ile-Leu-Asp-Asp-Arg 110 120-Cys-Len-Asn-Gly-Len-Arg-Glu-Thr-Tyr-Gln-Ala-Leu 130-Gly-Val-Pro-Gly-Ala-Ser-Val-Ala-Val-Gly-Ile-Glu 140-Lys-Met-Lys-Asp-Ser-Ala-Ile-Ala-Ile-Ala-Asn-Asp 150-Pro-Ser-Gly-Ile-Thr-Thr-Gly-Asp-Cys-Ser-Ala-Len 160-Met-Ala-Glu-Val-Gly-Thr-Tyr-Phe-Asp-Arg-Ala-Ala 170-Thr-Ala-Val-Glnγ ( Tyr ) ( Pro ) ,。
In this explanation, amino acid, polypeptide and active group etc. are all represented with the form of abbreviation or code name by the rule of International Union of Biochemistry (IUPAC).The example of these abbreviations or code name is as follows:
Arg: arginine Ala: L-Ala Asn: N Asp: aspartic acid
Cys: halfcystine Gln: glutamine Glu: L-glutamic acid Gly: glycine
His: Histidine Ile: Isoleucine Leu: leucine Lys: Methionin
Met: methionine(Met) Phe: phenylalanine Pro: proline(Pro) Ser: Serine
Thr: Threonine Trp: tryptophane Tyr: tyrosine Val: Xie Ansuan
The present invention adopts from animal, plant, the hydrolysising protease that obtains in microorganism and the genetically modified microorganism, in acidity, in alkalescence or the neutral environment or by means of calcium, magnesium, phosphorus, selenium, zinc, under the condition that mn ion participates in, hydrolysis temperature is at 20～80 ℃, optimum temps is at 30～60 ℃, the lytic enzyme that adopts has following source: as stomach en-, trypsinase, N,O-Diacetylmuramidase etc.: plant origin: papoid, bromeline, ficin or the like: genetically modified microorganism: as the Flavorzyme of Novo Nordisk company, Alcalase hydrolysising protease etc., the proteic feed ratio of lytic enzyme and algae 1: 1～100, best 1: 25～40, hydrolysis time 1～50 hour, best 4～20 hours.Spirulina is contained algae albumen carry out one or many hydrolysis step by step, to obtain to have the single of physiologically active or several polypeptide mixture, general peptide bond number is no more than 50, and majority is 2～10 little peptides.Its preparation technology's flow process is pressed Fig. 1 or Fig. 2, method for hydrolysis, can one the step, also can be rapid, or press Fig. 1 or Fig. 2 method and intersect and use for multistep, with the purpose peptide of acquisition maximum yield.Method such as the solvent extration used always when the polypeptide that obtains by above-mentioned the whole bag of tricks can be according to isolated polypeptide, apportion design and post layer method etc. is separated and purifying, thereby obtains each peptide species of the present invention and various polypeptide mixture.
Various polypeptide mixture of the present invention are carried out some separating treatment, can obtain high F value spirulina oligopeptides.F value (Fischer) is meant branched-chain amino acid in the protein molecule (Branchcdchainaminoacids is called for short BCAA), with the die aromatischen Aminosaeuren molar ratio of (Aromatic amino acids is called for short AAA).High F value oligopeptide is the non-polymeric peptide of a kind of small molecular weight of forming behind the proteolysis; Wherein branched-chain amino acid occupies larger proportion, thereby has special physiological properties.From soybean protein, whey-protein, the experiment of obtaining high F value oligopeptide in fish-protein and the zein has had report.The present invention prepares high F value spirulina oligopeptides with spirulina as protein source, does not appear in the newspapers as yet.
So the polypeptide of the present invention that obtains exists with the form of free state or its salt usually, and have with mutually seemingly should the similar biological activity of original natural polypeptides.Therefore, they can be used as various medicinal products or healthcare products.Can according to a conventional method they be changed into and to make medicinal acid salt or mixture.Perhaps natural polysaccharide such as spirulina polysaccharide, lentinan or endoxan, methotrexate, 5 FU 5 fluorouracil ... pressing on described one group of little peptide Deng scion grafting, this organizes little peptide bond number is 2～11.This organizes little peptide can become the tumour cell affinity peptide according to its effect, they play a kind of targeting vector effect, antitumorigenic substance through its delivery has enrichment phenomenon in tumour cell, improved bioavailability of medicament, strengthened the antitumor curative effect of medicine, make antitumor drug play the antitumor curative effect of high dosage at low dosage level, and when not having high dosage to human normal cell's toxic side effect, significantly improve treatment security and validity.
Being used to prepare above-mentioned salifiable example has: mineral acid (Hydrogen bromide, hydrochloric acid, phosphoric acid), organic acid (formic acid, acetate, propionic acid, lactic acid, oxalic acid, succsinic acid, oxysuccinic acid, tartrate, citric acid, phenylformic acid, Whitfield's ointment, Phenylsulfonic acid, nicotinic acid, ursolic acid, xitix, nonane diacid, oleic acid, linolic acid).Can prepare above-mentioned mixture by the inorganic formula organic substance that makes polypeptide have better physiologically active that adds some type.The inorganics that is used to form these mixtures comprises phosphoric acid salt, pyrophosphate salt, the palmitate of mineral compound, especially these metals of metals such as calcium, magnesium, zinc, selenium, cobalt, with and oxyhydroxide, the polyphosphate of other metal.Described organic examples of substances has: nonantigenic gelatinum, natural polysaccharide such as spirulina polysaccharide, astragalus polysaccharides, lentinan, phytinic acid, polyglutamic acid, inosine etc.
According to the purpose of treatment, the type of medicament can be selected in very wide scope, and the example of medicinal compositions has tablet, powder, liquid, suspension, particle, capsule, suppository, nose dropping liquid, oral mucosa absorption agent (as sublingual tablet, chewable tablets, lozenge, and dropping liquid), and ointment.
For the preparation processing with this polysaccharide medicine becomes tablet, can make medicinal supporting agent, for example: vehicle, as sucrose, lactose, sodium-chlor, glucose, fructose, urea, starch, lime carbonate, wilkinite, Mierocrystalline cellulose, silicic acid etc., tackiness agent such as water, ethanol, syrup, starch solution, gelatin, lac, methylcellulose gum, polyvinylpyrrolidone etc., disintegrating agent such as sodiun alginate, the agar powder, the kelp powder, lime carbonate, Calcium hydrogen carbonate, polyoxyethylene, sorbitan-fatty acid ester, stearic acid monoglycerides, sodium lauryl sulphate etc., disintegration inhibitor such as sucrose, the hydrogenation wet goods, absorption enhancer such as quaternary amine, sodium lauryl sulphate, the tall and erect ketone of dodecyl nitrogen etc., wetting agent such as glycerine, propylene glycol etc., lubricant such as stearate, polyoxyethylene glycol etc.When the preparation tablet, the coating material of available routine is made the tablet coating sheet again, to make coated tablet, gelatin film coating tablet, ECT, also can be made into the tablet or the bilayer tablet of film dressing.
For these polypeptide drugs are made pill, also can use the known carrier of pharmacy field, vehicle such as lactose, glucose, starch, hydrogenated vegetable oil, talcum powder, kaolin, tackiness agent such as gum arabic, tragcanth, gelatin etc., disintegrating agent as, agar, kelp etc.
In order to make capsule, can receive polypeptide of the present invention and mix with above-mentioned carrier, the mixture that obtains is thus inserted in the gelatine capsule of medicinal specification or in the soft capsule, so that make capsule.
In order to be processed into injection formulations, be to the solution of these polypeptide active components, emulsion or suspension carry out sterilization, can add thinner, as water, ethanol, polyoxyethylene glycol, polysorbate40, tween 80 etc.
For required preparation is penetrated in the blood of human body, can adds an amount of tackiness agent, thinner, propelling agent, absorption agent etc. and make powder agent, aerosol-type and liquid-type or semi-solid type preparation.
In order to be processed into emulsion, paste or gelifying agent, also can use pharmaceutical excipient vast in this field, as white vaseline, whiteruss, glycerine, polyoxyethylene glycol, silicone, card uncle glue, sodium lauryl sulphate etc.
Administering mode to the said medicine preparation does not have special restriction, can be according to dosage form, patient's sex age and other situations, the degree of symptom etc. is selected for use, for example tablet, pill, suspension agent, emulsion, granule and capsule can be oral, but inject intravenously administrable, or mix with physiological saline, glucose emulsion.But administration under muscle, skin and flesh, the epidermis if desired, nasal drop can be used for the oral mucosa position by the administration of suction nasal cavity, oral mucosa absorption-type.
In order to illustrate in greater detail the present invention, will enumerate three embodiment below, but not limit the scope of the invention.
Embodiment one
Press Fig. 2 method, the enzyme pre-treatment: pancreatin, trypsinase U
2, papoid U
3Press U1: U2: U3=1: 3～6: 1, add deionized water, handle with 9% glucose solution and 0.17% calcium chloride solution, be incubated 2 hours down at 28 ℃, standby.
Get spirulina powder 1000 grams, add 6 liters in water, fully stir, use 0.1 hydrochloric acid soln or 5% sodium carbonate solution adjust pH to 8 respectively, boiled 1 minute, be chilled to 45 ℃.Above-mentioned enzyme solution is pressed enzyme-to-substrate concentration ratio E/S=6000U/g amount add fully stirring in the spirulina suspension, 45 ℃ are incubated 2～6 hours.Get enzymolysis solution 1.Enzymolysis solution 1 was boiled 2～10 minutes, and adjust pH to 7 is collected filtrate and filter residue respectively.The deionized water that adds 1.5～4 times in this filter residue, adjust pH to 8 is pressed Fig. 1 method, presses enzyme-to-substrate concentration ratio E/S=6000U/g amount and adds trypsin solution, fully stirs at 45 ℃, is incubated 2～6 hours, gets enzymolysis solution 2.Enzymolysis solution 2 is boiled 2～10 minutes adjust pHs to 7, collect filtrate and filter residue respectively.Merge enzymolysis solution 1 and enzymolysis solution 2 filtrates, adjust pH to 7 is pressed Fig. 1 method, presses enzyme-to-substrate concentration ratio E/S=4500U/g and measures adding papoid solution 50 ℃ of abundant down stirrings 3 hours, gets enzymolysis solution 3.With enzymolysis solution 3 adjust pHs to 7, boiled 2～10 minutes, collect filtrate respectively and consider slag.Enzymolysis solution 3 is added 5% sodium chloride solution that is equivalent to spirulina dry product weight 0.07～0.04% boiled 1 minute, stir evenly the back standing over night.Abundant refrigerative filtrate bottom throw out emitted to stay use it for anything else, the top clear liquor is active polypeptide stoste.This stoste is surpassed the worry membrane sepn with Dtaflo PM-30 type, and gained filtrate contains oligopeptide 4～11%.This oligopeptide solution may be made in tablet, capsule after drying, can make after the adding seasonings oral liquid (need be concentrated into 0.9～1.0 proportion) or can Chinese medicine (Chinese medicine is effectively abundant) as spirulina polysaccharide, astragalus polysaccharides, lentinan, or other drug such as succsinic acid, oxysuccinic acid, tartrate, citric acid, phenylformic acid, Whitfield's ointment, Phenylsulfonic acid, nicotinic acid, ursolic acid, xitix, nonane diacid, oleic acid, linolic acid.Can prepare above-mentioned mixture by the inorganic formula organic substance that makes polypeptide have better physiologically active that adds some type.The inorganics that is used to form these mixtures comprises phosphoric acid salt, pyrophosphate salt, the palmitate of mineral compound, especially these metals of metals such as calcium, magnesium, zinc, selenium, brill, with and oxyhydroxide, the polyphosphate of other metal.Described organic examples of substances has: nonantigenic gelatinum, compatibility agent.
Determination of protein concentration with UV-light 215nm and 255nm absorption difference method, can be measured the concentration of contained oligopeptide in the solution.Be calculated as follows: protein concn (mg/ml)=0.144 (A215～A255).The lipidated protein check: use ultraviolet spectrophotometry, absorption value A280/A260 should be 1.75.
Embodiment two
Preparation has the bioactive peptide of hypertension pharmacy from the algae albumen of spirulina.
Press Fig. 2 method, spirulina powder 1000g adds 6 liters in water and becomes solution to transfer to pH5.5 with formic acid solution, boils 10 minutes.(make bromeline (self-control, enzymic activity＞3.5 * 106 μ/g μ μ) and papoid by oneself enzymic activity＞2500 μ/g) respectively in 1: 5～10 ratios adding bromeline 6 * 10 soluble in water
6μ and papoid 4 * 10
6: 45 ℃ are stirred insulation 3h hour, and 50 ℃ are stirred insulation 1h, then the bright liquid of enzyme are boiled 2～10 minutes, transfer to pH7 (using 5% yellow soda ash).Collect filtrate and filter residue respectively.Filter residue can be used as the raw material of next enzymolysis.It is an amount of to add sal epsom in collecting filtrate, and under reduced pressure urea filtrate is carried out spraying drying powder-forming with concentrated solution.The spirulina multiple polypeptides mixture that adopts this technology to obtain has antihypertensive function, and the general-purpose industrial formulation method is made various above-mentioned preparations routinely.
Embodiment three
Prepare high F value spirulina oligopeptides by Fig. 1 method.
Spirulina powder 1000g adds 6 liters in water and becomes solution to transfer to pH7.5 with formic acid solution, boils 10 minutes.Be chilled to 70 ℃, Alcalase (2.4L) the hydrolysising protease 6ML that adds Novo Nordisk company, fully stir, be incubated 2 hours, boil sterilization in 5 minutes, obtain hydrolyzed solution 1, be cooled to 50 ℃, (20m Mol/L, pH4.0), perhaps pH value to 6 is separated in 5% sodium carbonate solution (being determined on a case-by-case basis) water transfer with methanol solution.Flaborzyme (1000ml) the hydrolysising protease 4ML that adds Novo Nordisk company stirs evenly insulation 2～8h, is warming up to 85 ℃ of sterilizations 5 minutes, while hot suction filtration.Add in the filtering hydrolyzed solution with Citric Acid, stir evenly, adjust pH to 4.8, and hydrolyzed solution is cooled to 60 ℃.Adopt activated carbon column, carbon/liquid=4: 10, temperature 60 is removed die aromatischen Aminosaeuren, presses 1: 3 mixed wash-out with 1N ammoniacal liquor and dehydrated alcohol then.Collect the part elutriant that there is maximum absorption at the 220nm place.Above-mentioned elutriant was evaporated to proportion 1: 10 under 80 ℃, through vacuum-drying, to moisture below 15%, again through microwave drying to containing moisture 4.5% following dry powder automatic analyzer for amino acids and electrophoretic analysis, can measure the oligo peptide that above-mentioned main products is made up of branched-chain amino acid, can be made into tablet.Capsule, formulations such as oral liquid.Be used for hepatic coma brain patient's nutritional support and strenuous exercise person's antisecosis, and the large-area burns patient, the wound person's of losing blood nutritional support.
Claims (5)
1, adopts the chemistry method that combines with biological enzymolysis, the algae albumen of spirulina is hydrolyzed, prepare the multiple little peptide and the high F value spirulina oligopeptides that have more various physiologically actives than original algae albumen, as the functional component or the medicine of functional health care food.
2,, adopt chemical process to be meant during hydrolysis algae albumen, in acidity, alkalescence or neutral environment, by magnesium, calcium, phosphorus, selenium, zinc, the isoionic hydrolyzation catalysis effect of manganese according to claim 1.The hydrolysising protease of biological enzymolysis is an animal-origin: as stomach en-, trypsinase, N,O-Diacetylmuramidase etc.; Plant origin: papoid, bromeline, ficin or the like: genetically modified microorganism: as the Flavorzyme of NovoNordisk company, Alcalase hydrolysising protease etc.
3, according to claims 1 and 2, hydrolysis temperature is at 20～80 ℃, and is best at 35～65 ℃, the feed ratio of lytic enzyme and proteolytic enzyme 1: 1～100, preferably 1: 25～40, hydrolysis time 1～50 hour, best 4～20 hours.
4, according to the little peptide of claim 1,2 and 3 gained, its acid salt and its mixture be have hypotensive, antitumor, decreasing cholesterol, reducing blood-fat, hypoglycemic, the preventing cardiovascular disease effect, fat-reducing is arranged again, prevent obesity, be used for hepatic coma brain patient's nutritional support, strenuous exercise person's antisecosis, and the large-area burns patient, the wound person's of losing blood nutritional support etc., the pharmaceutical composition of multiple physiologically active.
5, according to claim 1,2,3 and 4 little peptide medicine composite is characterized in that: it contains at least a little peptide, and its acid salt or its mixture are as the described physiological activity of claim 4.
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| CN 98102406 CN1204651A (en) | 1998-06-08 | 1998-06-08 | Preparation method for spirulina polypeptide and medicament containing these polypeptide and application |
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| CN 98102406 CN1204651A (en) | 1998-06-08 | 1998-06-08 | Preparation method for spirulina polypeptide and medicament containing these polypeptide and application |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2857978A1 (en) * | 2003-07-25 | 2005-01-28 | Simer Laboratoires Science Et | Preparing a peptide extract of Spirulina, useful in nutraceutical and cosmetic compositions for e.g. controlling aging of the skin, comprises extraction of lipids then enzymatic hydrolysis |
| CN102961733A (en) * | 2012-11-16 | 2013-03-13 | 福建省水产研究所 | Application of seaweed polypeptide in preparing health-care product for reducing blood fat and blood sugar as well as beverage containing polypeptide for reducing blood fat and blood sugar |
| CN104120160A (en) * | 2014-06-10 | 2014-10-29 | 中国计量学院 | Preparation method for chlorella anti-oxidative peptide |
| CN106901372A (en) * | 2016-12-28 | 2017-06-30 | 陕西天宝大豆食品技术研究所 | Full spirulina peptide nutrient food and preparation method thereof |
| CN107893095A (en) * | 2017-12-19 | 2018-04-10 | 大连深蓝肽科技研发有限公司 | A kind of method that marine source albumen prepares high F value oligopeptide |
| CN108624643A (en) * | 2018-04-02 | 2018-10-09 | 中国科学院青岛生物能源与过程研究所 | The method for preparing high added value oligopeptides as raw material using micro- quasi- ball algae-residue |
| CN109536557A (en) * | 2018-12-31 | 2019-03-29 | 山东好当家海洋发展股份有限公司 | A kind of preparation method of phycobniliprotein polypeptide powder |
-
1998
- 1998-06-08 CN CN 98102406 patent/CN1204651A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2857978A1 (en) * | 2003-07-25 | 2005-01-28 | Simer Laboratoires Science Et | Preparing a peptide extract of Spirulina, useful in nutraceutical and cosmetic compositions for e.g. controlling aging of the skin, comprises extraction of lipids then enzymatic hydrolysis |
| CN102961733A (en) * | 2012-11-16 | 2013-03-13 | 福建省水产研究所 | Application of seaweed polypeptide in preparing health-care product for reducing blood fat and blood sugar as well as beverage containing polypeptide for reducing blood fat and blood sugar |
| CN104120160A (en) * | 2014-06-10 | 2014-10-29 | 中国计量学院 | Preparation method for chlorella anti-oxidative peptide |
| CN104120160B (en) * | 2014-06-10 | 2017-05-24 | 中国计量学院 | Preparation method for chlorella anti-oxidative peptide |
| CN106901372A (en) * | 2016-12-28 | 2017-06-30 | 陕西天宝大豆食品技术研究所 | Full spirulina peptide nutrient food and preparation method thereof |
| CN107893095A (en) * | 2017-12-19 | 2018-04-10 | 大连深蓝肽科技研发有限公司 | A kind of method that marine source albumen prepares high F value oligopeptide |
| CN108624643A (en) * | 2018-04-02 | 2018-10-09 | 中国科学院青岛生物能源与过程研究所 | The method for preparing high added value oligopeptides as raw material using micro- quasi- ball algae-residue |
| CN109536557A (en) * | 2018-12-31 | 2019-03-29 | 山东好当家海洋发展股份有限公司 | A kind of preparation method of phycobniliprotein polypeptide powder |
| CN109536557B (en) * | 2018-12-31 | 2024-02-09 | 山东好当家海洋发展股份有限公司 | Preparation method of phycobiliprotein polypeptide powder |
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