CN1203091A - Swallow's pad polyose - Google Patents

Abstract

A crassula poitulacea polysaccharide (CPS) as health-care product for preventing inflammation and cancer and improving immunity is extracted from fresh crassula poitulacea or eucalyptus by special biochemical technique. Said CPS has no toxic by-effect to animals and human body. This invention is also suitable for production in Chinese pharmaceutical factory.

Description

Crassula argentea polgsaccharide

Crassula argentea Thunb is a kind of wild or tame meat Crassulaceae plant, the mixed composition of finding its extraction the earliest has hypoglycemic function (the flat 5-19554 of special permission communique), after this find again that its main blood sugar lowering effective ingredient is chemical compounds such as Crassula argentea Thunb glycoside (special permission communique, flat 8-2535674) class.The current Crassula argentea polgsaccharide of finding (Crassula argentea polysaccharide is abbreviated as CPS).Through studying for a long period of time and clinical discovery, use the Crassula argentea polgsaccharide (CPS) that the high-tech biochemical method extracts and both can be developed into antiinflammatory, the immunologic function that improves human body and the health food for the treatment of the auxotype of all kinds of cancers, also can be developed into antiinflammatory, enhancing immunity, anticancer medicine.

Crassula argentea polgsaccharide of the present invention (CPS) is extracted from Crassula argentea Thunb and cajaput *, relates in health product and the field of medicaments prevention of inflammation, cancer and to improve on the immune function of human body be an important breakthrough.

The present invention directly extracts Crassula argentea polgsaccharide from Crassula argentea Thunb or cajaput, also can extract Crassula argentea Thunb or cajaput polysaccharide indirectly from the former powder of Crassula argentea Thunb indirectly.

Now from Crassula argentea Thunb or cajaput or the former powder of Crassula argentea Thunb, extract the method for CPS, after the protective effect of observation experiment, immunology observation experiment, body injury and the reparation of the acute toxicity of CPS and subchronic toxicity testing, anti-inflammatory, external and the interior cancer resistant effect of body is tested and clinical model case is specified in respectively.

One, extracts the method for CPS

Now with root, stem, leaf and the Herb of Crassula argentea Thunb or from the former powder of Crassula argentea Thunb, extract Crassula argentea polgsaccharide (CPS) with diverse ways respectively:

Embodiment 1.Bright Crassula argentea Thunb root 2kg, after the pulverizing, alcohol reflux defat 16h extracts 8h with 20L water at 90-100 ℃ after the residue drying, and the centrifugal supernatant of telling in cooling back repeats 5 times, merges supernatant, is concentrated into small size.Add 3 times of ethanol, the gained resolution of precipitate is in water, with 15% trichloroacetic acid Deproteinization, the dialysis of among the 2mol/L NaOH and back concentrates, and adds 3 times of volume ethanol, the gained precipitation is used for dehydrated alcohol, acetone, ether washing successively, obtains crude polysaccharides 42g, is the taupe brown powder.Get the 10g crude polysaccharides and be dissolved in the 100ml water, carry out DEAE-cellulose column chromatography (Cl ^-Type, the first water eluting of 90cm * 3.0cm), again with 0-2mol/L NaCl gradient elution, water elution partly obtains CPS11.8g, the NaCl eluting partly obtains 5.1gCPS, with 16.9g CPS again through Sepharose 4B column chromatography (50cm * 2.0cm), 0.05mol/L NaCl eluting, obtain the 13.8g of purification, productive rate 0.69%.

Embodiment 2.The bright Crassula argentea Thunb leaf of 2kg is smashed to pieces after the water that adds 8-10 times of volume behind ether defatting extracting 5 times in boiling water bath, merging filtrate, and with Sevage method deproteinization, decolouring back 10L85% ethanol precipitation, dry getting ^*Cajaput and Crassula argentea Thunb belong to jade plant together and belong to, and profile fairly similar, wherein chemical effective ingredient are also identical substantially, only are not of the same race.Crude polysaccharides CPS is dissolved in CPS in the hot water, and (2.8cm * 37cm), water and 0-4mol/L NaCl carry out gradient elution successively through DEAE-cellulose (DE-52) column chromatography.(1.5cm * 96cm), with 0.01mol/L NaCl eluting, flow velocity 12ml/h detects polysaccharide, pure product: 12.9g, productive rate: 0.645% with sulphuric acid-anthrone method to Sephadex G-100 gel filtration.

Embodiment 3.After getting the bright Crassula argentea Thunb stem pulverizing of 2kg, hot water (90 ℃) extracts 5 times, merges 5 filtrate decompression and concentrates the back with 4 times of 95% alcohol chromatography, and centrifugal, conventional dehydrate gets crude polysaccharides (CPS).Crude polysaccharides 2% aqueous solution-30 ℃ freezes fully, and room temperature is put to just melting fully, and with the centrifugal precipitation of going of the western Hunan-Towy2 ℃ of 8000r/min of High speed refrigerated centrifuge GL-20, supernatant repeats freeze thawing 3 times, till not having a precipitation.Supernatant after the freeze thawing directly carries out alcohol grading, (polysaccharide solution: 95% ethanol V/V) ethanol precipitation was carried out at each interval 0.25 to 1: 3.0 from 1: 0.5, wherein for main level lease making pronase method and butanols chloroform (1: 4) method associating deproteinization get polyoses content be 99.8% CPS at 1: 1.25, obtain product weight: 13.95g, productive rate: 0.698%.

Embodiment 4.After getting the bright Crassula argentea Thunb pulverizing of 2kg Herb, place pressure cooker and add 2kg water and be heated to 2kg pressure, repeat 5 times, merge extractive liquid,, concentrate, filter, dialysed 48 hours, add triplication 95% ethanol, centrifugal, precipitation is 95% ethanol in succession, washing with acetone, vacuum drying, get the CPS semifinished product, semifinished product is dissolved in a small amount of distilled water, centrifugal, supernatant adds Sephadex G-100 chromatographic column, use the distilled water eluting, the mountain portions of fraction collection anthrone method polysaccharide positive reaction, water-bath concentrates, added the calcium hydrogen phosphate stirring and adsorbing 3 hours, centrifugal, precipitation is resolved with diluted acid, and is centrifugal again, the supernatant dialysis is to neutral, with Sevag method Deproteinization, add 3 times of amount 95% ethanol, centrifugal, supernatant carries out single step purification through Sephadex G-200 chromatographic column, use the distilled water eluting, as the mountain portions of above-mentioned fraction collection polysaccharide reacting positive, water-bath concentrates, and adds triplication 95% ethanol, centrifugal, precipitation is in succession with 95% ethanol, washing with acetone, vacuum drying get the Powdered Crassula argentea polgsaccharide of taupe brown (CPS) highly finished product 13.85g, productive rate: 0.693%.

Embodiment 5.Get the former powder of 100g Crassula argentea Thunb and be suspended in the 200ml distilled water, 1 hour after-filtration of heated and boiled, aforesaid operations repeats 4 times, merge 5 times filtrate, be chilled to room temperature after, with 70% ethanol 1000ml precipitating proteins, after the filtration, filtrate reuse 85% ethanol 1000ml precipitates polysaccharide, and after the filtration, filtrate reuse 95% ethanol 1000ml precipitates polysaccharide, filter, filtrate obtains crude polysaccharides * (CPS) 19.1g with 98% ethanol 500ml precipitation polysaccharide (containing a small amount of proteoglycan), and productive rate is 19.1%.* the molecular weight of thick Crassula argentea polgsaccharide (containing a small amount of Crassula argentea Thunb proteoglycan) is greater than 10,000, and content is more than or equal to 25%.

Two, the zoopery of CPS:

1, acute toxicity test:

(1) gets 50 of body weight 18-22g Kunming mouses, carry out LD according to a conventional method ₅₀Measure (intraperitoneal administration), the result is 967.00 ± 143.15mg/kg (credibility is greater than 95%).

(2) subchronic toxicity test (be 90 days experimental period)

Get 4 of healthy dogs, about the about 10kg of body weight, divide two groups of contrast and administrations, intravenous administration.The result shows: before and after the administration, Canis familiaris L. body weight, appetite, spirit and hepatic and renal function, hemogram are all normal.Main organs (samples such as the heart, liver,spleen,kidney, lung, stomach, intestinal, pancreas and adrenal gland) appearance not damaged, tissue slice there is no because drug-induced pathological changes and intoxicating phenomenon.Pair minimum or nontoxic pair effect of effect of CPS poison is described.

2, CPS is to the facilitation of immunity

(1) CPS is to the influence of immune organ weight

Get 20 of Kunming mouses about body weight 16g, divide two groups of contrast and administrations, male and female half and half.Administration group lumbar injection CPS, every 2mg * 7d, matched group is given the equivalent normal saline, and administration finishes the back dissects next day, with the cervical region depletion method mice is put to death.Taking-up spleen and thymus is also weighed, and the results are shown in Table 1.

Table 1 CPS is to the influence of mouse spleen and thymic weight

Polysaccharide	The Mus number	Route of administration	Dosage mg/kg * d	Spleen index ± SD (mg/10wt)	Thymus index ± SD (mg/10wt)
						Matched group	????11	????ip	???-	????61.30±3.31	??44.92±3.52
CPS	????11	????ip	???100×7	????89.56±8.03 *	??40.88±3.61 **

*P＜0.01，**P＞0.05

By table 1 as seen, CPS can obviously increase mouse spleen weight, compares with matched group, and spleen index increases by 0.46 times, but not obvious to the influence of thymus.

(2) CPS is to the influence of macrophage phagocytic function

Get 20 of body weight 18-22g Kunming mouses, with creep plate method (pharmacological experimental methodology, 1981,934 pages, People's Health Publisher, Beijing.) observe the influence of CPS to macrophage phagocytic function, the results are shown in Table 2.

Table 2 CPS is to the influence of peritoneal macrophage phagocytic function

Polysaccharide	The Mus number	Route of administration	Dosage mg/kg * d	Engulf percent ± SD	Phagocytic index ± SD
						Matched group	???10	????ip	??-	???57.40±2.6	??1.38±0.09
CPS	???10	????ip	??100×7	???90.0±1.6 *	??4.37±0.34 **

*P＜0.001

By table 2 as seen, CPS can obviously strengthen the phagocytic function of mouse macrophage, compares with matched group, and phagocytic index has increased by 3.17 times, and engulfing percent increases by 1.57 times.

(3) CPS is to the influence of humoral immunization

Get 20 of the purebred mices of body weight 18-22g C57/BL6, press literature method (medicine journal 1979,14:443-446.) measure CPS to the influence that serum hemolysin produces, the results are shown in Table 3.

The influence that table 3 CPS produces hemolysin

Polysaccharide	The Mus number	Route of administration	Dosage mg/kg * d	Half hemolysis value ± SD HC 50±SD
					Matched group	???10	????ip	????-	????16.35±3.81
CPS	???10	????ip	????50×7	????23.24±5.81 *

*P＜0.01

By table 3 as seen, CPS has the effect that increases half hemolysis value, and compares half hemolysis value (HC ₅₀) increase by 0.56 times approximately.

(4) influence of CPS pair cell immunity

1. E rosette experiment: get 20 of centrifuge tubes, delivery tube adds 0.2ml (25 μ g/ml) CPS, and control tube adds 0.2ml Hank ' s liquid, and (Jiangsu Province's pharmacopeia (1982) thymosin is injected provisional quality standard by literature method.87 pages) observed CPS to the influence that the E rosette forms, the results are shown in Table 4.

The influence that table 4 CPS forms the E rosette

Polysaccharide	The pipe number	Dosage μ g/ml	E rosette formation rate ± SD
				Matched group	???10	????????-	????38.2±1.5％
CPS	???10	????????25	????61.1±1.7％ *

*P＜0.01

By table 4 as seen, CPS can obviously increase the formation rate of E rosette, compares with matched group, and formation rate can increase by 0.80 times.

2. (Acta Biochimica et Biophysica Sinica 1979,11:145-150 are tested in lymphocyte transformation.): get fresh normal person's whole blood (anticoagulant heparin) 0.2ml, add contain the 2ml culture fluid (include RPMI 1640, each 100 μ/ml of mycillin, adding calf serum, to make into concentration be 20%, uses NaHCO ₃Transfer pH7.4) test tube in, divide three groups to be matched group (normal saline), CPS (50 μ g), thick CPS organizes 50 μ g, 37 ℃ hatch 56h after every pipe add 5 μ Ci ³H-UR continues to hatch after shaking up to 72h, and with 49 type glass fibre filter disc harvestings, counting the results are shown in Table 5 with FJ-2105 liquid scintillation instrument rolling counters forward DPM.

The influence that table 5 CPS transforms normal person's Whole blood lymphocyte

Polysaccharide	Dosage	????DPM±SD	Stimulation index
				Matched group	??-	????238456±62712	?????-
????CPS	??100	????503065±112840 *	?????2.11
				Thick CPS	??50	????541826±156573 *	?????2.01

*P＜0.01

By table 5 as seen, the comparison of CPS group incorporation is shone and is increased by 1.11 times, but all has the LT effect of promotion with thick CPS comparative descriptions, there is no evident difference each other.

(5) CPS is to the erythrocytic agglutination of rat

Get Wei Sita kind rat, heart puncturing extracting blood, anticoagulant heparin is pressed literature method (Acta Biochimica et Biophysica Sinica, 1981,13:121-124.) experimentize, the results are shown in Table 6.

The influence of table 6 pair rat erythroagglutination

Polysaccharide	Sample number	Dosage mg/ml	????E±SD	Coagulation percentage rate %
					Matched group	????8	????-	??0.474±0.088	????-
CPS	????8	????1	??0.270±0.031 *	????42.2％

*P＜0.01

As shown in Table 6, CPS has the erythrocytic effect of coagulation, illustrates that it has the agglutinin effect, participates in cytophagous activation and biophylaxis.

3, CPS is to the reparation and the protective effect of body injury

(1) CPS is right ⁶⁰The radiating protective effect of Co gamma-rays

Symptoms such as get 50 of the kunming mices of body weight 22g, after the administration mice is shone, accumulated dose is 800 roentgens, shines after three days, and few food, few drink, loose stool appear in mice, loose nothings of fur is damp and lose weight were observed one month, the results are shown in Table 7.

By table 7 as seen, CPS has the certain protection effect to radiation, compares with matched group, and mean survival time improves 0.80 times.

(2) effect of the leukopenia of CPS antagonism caused by cyclophosphamide

Get 20 of the Kunming mouses of body weight 22g, administration group lumbar injection CPS to the 7 days, other injects cyclophosphamide 2mg, continues administration then two days, checks leukocyte count routinely before and after the administration, the results are shown in Table 8.

By table 8 as seen, CPS has the effect of the leukopenia that obvious antagonism causes by cyclophosphamide.

Table 7 CPS is right ⁶⁰The radiating protective effect polysaccharide of Co gamma-rays Mus is counted route of administration dosage mg/kg * d mean survival time (d) ± SD matched group 25 ip---6.36 ± 2.95CPS, 25 ip 100 * 7 11.44 ± 5.83 ^** P＜0.01 (ip is a lumbar injection)

The effect of the leucocytes reduction that table 8 CPS antagonism cyclophosphamide causes

Polysaccharide	The Mus number	Dosage mg/kg * d	Leukocyte count ± SD
						Before the administration	After cyclophosphamide poisons	Rate of descent
			Matched group	??10	??-				???6570±571	????2315±290	?64.8±1.0
CPS	??10	??100×9				???7230±658	????5765±515	??20.3±3.3*

P＜0.001

4, the antiinflammatory action of thick CPS

Get 10 of body weight 250-300g Wei Sita kind rat, press document (pharmacological experimental methodology, 526 pages, People's Health Publisher, Beijing, 1982.) rat pedal swelling method experimentizes.Ovum Gallus domesticus album solution with 0.1ml 10% causes inflammation, the results are shown in Table 9.

Table 9 CPS is to the effect of anti-inflammatory

Polysaccharide	The Mus number	Route of administration	Dosage mg/kg	Cause scorching back swelling rate ± SD
								??1hr	????3hr	????5hr	??7hr
				Matched group	??5	????ip	?-					?54.7±3.7	?54.3±2.0	?41.6±6.0	?23.8±4.1
Thick CPS	??5	????ip	?60					?30.5±3.8 *	?26.1±4.4 *	?14.2±4.6 *	?9.8±3.4

P＜0.01

By table 9 as seen, CPS promptly shows preferably effect to anti-inflammatory causing scorching back 1h.

5, CPS is right ³The H-leucine mixes the influence of serum albumin

Get 20 of body weight 14-16g Kunming mouses, administration group lumbar injection CPS100mg/kg * 4d, fasting 16h before mixing, every Mus lumbar injection during experiment ³H-leucine 400 μ Ci/kg (Oura H (1972) Effect of radix ginsing extract on serum protein synthsis, Chem PharmBull 21:980-986.) the results are shown in Table 10.

Table 10 CPS is right ³The H-leucine mixes the proteinic influence of mice serum

Polysaccharide	The Mus number	Route of administration	Dosage (mg/kg * d)	CPM ± SD/0.1 ml serum albumin
					Matched group CPS	??9 ??10	????ip ????ip	????- ????135×4	????1625±136 ????2674±378 *

P＜0.01

By table 10 as seen, CPS can promote the proteic biosynthesis of mice serum, compares with matched group, and incorporation can increase by 65%.

Three, the anticancer experiment of CPS:

1, the external cancer that presses down of CPS is tested:

Experimental technique: tetramethyl azo azoles salt (MTT) colorimetry

Ultimate principle: MTT enters behind the cell as reaction substrate, the first a ceremonial jade-ladle, used in libation (Formazan) of the blue color of oxidized formation, be deposited in the cell or on every side, directly measure the OD value in each hole with enzyme mark photometer, can react the amount of first a ceremonial jade-ladle, used in libation, judge intracellular plastochondria oxidase active thus, but the also propagation level of indirect reaction cell.

Reagent: MTT[3-(4,5-dimethythiazal-2yl) 2,5-diphenyltetrazolium bromide] be made into CPSlmg/ml use liquid with the phosphate buffer (0.02mol/L, H7.2) that contains 0.5% glucose, 4 ℃ keep in Dark Place.

The acidify isopropyl alcohol contains the isopropyl alcohol of 0.04mol/L HCl.

The cell strain of experiment usefulness: Hela (going into cervical cancer cell), Bca-61 (human breast cancer cell), NFC (normal person fibroblast)

Experimental result:

Table 11 CPS is to in-vitro cell growth suppression ratio (%)

Drug level	??10％???5％????3％?????2％?????1％?????0.5％
		Hela Bca?61 NFC	??100????100????54.8????55.3????51.6????36.5 ??100????100????36.7????39.0????26.4????24.2 ??100????100????-???????10.4????0.0?????0.0

Experimental result shows: certain density medicinal liquid (1-2%) all has inhibitory action to the growth of testing used Hela and Bca-61 cell, and the growth of normal fibroblast (NFC) is not then had obvious influence.Medicinal liquid is more obvious to the performance of Hela cell, and the medicinal liquid of 1% concentration can make suppression ratio reach 55.3%, and this medicinal liquid truly has the value of further exploitation.

2, CPS vivo antitumor effect observation: animal is divided into five groups of blank, positive control, low dosage (20 μ g/ only/day), middle dosage (40 μ g/ only/day), high doses (80 μ g/ only/day) at random, animal is Kunming mouse male and female half and half, all inoculates s-180 ascites cells 1 * 10 except that the blank group ⁵, the blank group only adds the 0.2ml normal saline every day.Be 7 days experimental period.

The table 12 CPS observation group Mus of anticancer effect in vivo counts negative several the cancer number of animals ascites cells mean cancer suppressing ratio % of route of administration dosage

(1 * 10 ⁶/ when ml) blank 20 po 0 20 0 are positive according to dosage group 22 po 500 μ g 17 5 56.0 77.15 high dose group 20 po 1000 μ g 16 4 76.0 80.5 among 20 po, 0 20 20 110.4 0 low dose group, 17 po, the 100 μ g 7 10 48.8 41.3

Crassula argentea polgsaccharide has certain lethal effect to the s-180 ascites cells, and the high dose effect is better, and CPS might become the health care medicine of prophylaxis of cancer

3, CPS anticancer effect existence in 30 days laboratory observation: experiment content is identical with CPS vivo antitumor effect observation, and be 30 days experimental period.

[notes] po: irritate stomach or oral abbreviation letter

Table 13 CPS anticancer effect existence in 30 days laboratory observation

The experimental project group	The Mus number	S-180 ehrlich ascites carcinoma 1 * 105	The 8th day counting positive rate (%)	Anticancer rate (%)	Existence natural law average	Existence natural law t test value	The P value
								The blank group	??10	Do not inoculate	????-	???-	???30	????-	???-
Positive controls	??10	Inoculation	???100	???0	???14.4	????-	???-
								High dose group 100mg//day	??10	Inoculation	???20	???80	???25.4	???4.140	??＜0.01
Middle dosage group 50 μ g//days	??10	Inoculation	???30	???70	???22.5	???3.231	??＜0.01
								Low dose group 25 μ g//days	??10	Inoculation	???44	???56	???20.8	???2.858	??＜0.05

Four, conclusion and discussion

The method of the extraction of Crassula argentea polgsaccharide is carried out a series of research, and its crude polysaccharides, refining polysaccharide carried out acute and subchronic toxicity test, there is no because drug-induced unusual and pathological changes, with regard to immunologic function, CPS all shows the function that promotes cellular immunization and humoral immunization preferably, and have good radioprotective, anti-radiotherapy chemotherapy, leukocyte increasing, anti-inflammatory effect, and have higher activate the phagocytic capacity; Experimental results show that CPS has antitumor action preferably, therefore, CPS is not only a health food preferably, and may become a health care medicine preferably.

Five, typical patient for example

1, the king * *: the man, appointed company to pay manager in 48 years old, it is historical existing more than 8 years to suffer from hemorrhoid, has cured and recur, be made into the solution of 5 μ g/mls with Crassula argentea polgsaccharide in April, 1994, smear 3 time in the affected part of anus every day, one week the back fully recover, never recur in February, 1997.

2, Liu * *: the man, 56 years old, appoint the company manager, suffered from hemorrhoid historical existing more than 10 year, be made into 10 μ g/ml solution with Crassula argentea Thunb sugar May nineteen ninety-five, smears every day 3 times, promptly fully recovers after two weeks, never recurs in May, 1997.

3, the king * *: the woman, 61 years old, oral ulcer did not heal for many years, be the solution of 100 μ g/mls with Crassula argentea polgsaccharide concentration in January, 1996, gargles every day 3 times, and slightly stopped 2-3 minute in mouth, i.e. recovery from illness after 4 days, recurrence never at the beginning of 1997 6 months.

4, the summer * *: the woman, 38 years old, the grade teacher often felt uncomfortable near the hepatic region, CT is diagnosed as vascular tumor, and whether can not make a definite diagnosis is optimum or pernicious, takes 10mg/ sheet Crassula argentea polgsaccharide sheet October nineteen ninety-five, 3 of every days, with the CT check, found that hemangioma has disappeared after 2 months.

5, old * *: woman, 54 years old, cadre, long-term hospitalization, be diagnosed as immunologic hypofunction, often catch a cold, leukocyte is on the low side, the back in by the end of October, 1996, take the Crassula argentea polgsaccharide sheet of 3 * 10mg/ sheet every day, after 3 months, flu is few gradually, leukocyte returns to normal value, normal working work at the beginning of 1997 4 months.

6, high * *: woman, 65 years old, retired cadre, being diagnosed as gingival carcinoma in 1996 8 months shifts, the patient refuse the operation, after take Crassula argentea polgsaccharide 10mg/ sheet, take 8 every day, be that 1000 μ g/ml Crassula argentea polgsaccharide solution are gargled and stopped in mouth 5 minutes with concentration simultaneously, every day 4 times, check that to the end of the year 1996 focus dwindles, feel symptom and alleviate, it is no any unusual to live, existing still survival.

Claims (8)

1, the present invention is the natural nutrition type health product and the medicine of a kind of newfound antiinflammatory, anti-cancer, raise immunity, and this product is directly to extract with special biotechnology from bright Crassula argentea Thunb or cajaput, also can extract polysaccharide from the former powder indirect of Crassula argentea Thunb;

2, according to claim 1, the concentration of alcohol scope that precipitates Crassula argentea polgsaccharide (containing the Crassula argentea Thunb proteoglycan) from extracting solution is 85-98%;

3, according to claim 1, the temperature of its heated and boiled Crassula argentea Thunb is not less than 100 ℃ generally at 100-120 ℃;

4, according to claim 3, heated and boiled not only plays the effect of breaking cell wall, face and also be the dissolubility that increases Crassula argentea polgsaccharide or protein polysaccharide;

5, according to claim 1, the crude product of Crassula argentea polgsaccharide (proteoglycan) and elaboration no significant difference in zoopery;

6, according to claim 1, special Measurement for Biochemistry is meant different number of chemical reagent, through different complicated operations such as chromatographic column;

7, Crassula argentea polgsaccharide antiinflammatory of the present invention, anticancer, all kinds of its P of immunity test (0.01, have the difference of highly significant.

8, according to claim 7, clinical consumption is the 10-30 milligram for each person every day, and the content of Crassula argentea polgsaccharide (contain a small amount of Crassula argentea Thunb proteoglycan, and molecular weight being greater than 10,000) must (be equal to or greater than) 25%.The physiologically active that just has remarkable or highly significant.