CN1203085C - Method for extracting glycerrhizic acid from licorice - Google Patents
Method for extracting glycerrhizic acid from licorice Download PDFInfo
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Abstract
The present invention relates to a method for extracting glycyrrhizic acid from licorice, which comprises the following steps: firstly, a microwave-assisted extraction method is used for quickly extracting glycyrrhizic acid from licorice in high efficiency, and then, organic extraction agents, such as tributyl phosphate, trialkyl phosphine oxide, isooctyl alcohol, hexyl alcohol, isoamyl alcohol, etc., are used for solvent extraction; the pH value of the extracting solution of the glycyrrhizic acid is adjusted to 1.5 to 6 by hydrochloric acid or sulfuric acid in the process of extraction; after the solution is statically placed or a centrifugal phase splitting process is finished, the glycyrrhizic acid is enriched in organic phases; water is used as a solution, and then, the glycyrrhizic acid is inversely extracted by adjusting the pH value of the mixed solution to 6.0 to 12.0; finally, a water phase solution enriching the glycyrrhizic acid is obtained; then, active carbon is added into the water phase solution to discolor the water phase solution enriching the glycyrrhizic acid; the discolored solution is acidified, statically placed or centrifugally deposited and dried to obtain refined glycyrrhizic acid. The present invention has the advantages of simple operation, high efficiency of concentration and purification, less energy consumption, safety, reliability, low cost and good environment.
Description
Technical field
The present invention relates to a kind of method of from Radix Glycyrrhizae, extracting Potenlini.
Background technology
Potenlini (Glycyrrhizic Acid GA) is isolating the earliest saponin constituents from Radix Glycyrrhizae, is made up of glycyrrhetinic acid and two molecule glucose aldehydic acid, and its structure is:
The pharmacological action of bibliographical information (Radix Glycyrrhizae is in the deep exploitation and the comprehensive utilization of aspects such as medicine, Hu Jinfeng, Shen Fengjia, herbal medicine, 26,39-44,1995) Potenlini mainly shows: a) have Interferon, rabbit in induced animal and the human blood (IFN) effect.B) reducing blood lipid: reduce cholesterol level in the blood, the treatment atherosclerosis.C) AIDS virus resisting.Potenlini also is widely used in industries such as foods and cosmetics in addition.
Traditional leaching process generally adopts the method (Pharmacopoeia of the People's Republic of China (95 years versions)) of thermal backflow or boiling.The method of boiling is that Radix Glycyrrhizae is dipped in the water, and heated and boiled is directly carried out the extraction of Potenlini; Heat reflow method and boiling method are similar, just install condensation reflux unit additional, and water constantly carries out condensation cycle.These class methods are consuming time longer, generally must 5-6 hours, and complicated component in the extracting solution, and foreign matter content height such as starch, colloid, flavones are handled for being further purified of Potenlini and to have been brought trouble.When extracting solution is concentrated, need boil evaporation to vat liquor, energy consumption is big, and enrichment step is various, and Potenlini is easy to hydrolysis, oxidative deformation in evaporating concentration process, so the production cost of Potenlini improves greatly.The production technique of at present refining Potenlini mainly contains: methods such as crystallization process, ion exchange method, macroporous adsorbent resin method.Aforesaid method at first adopts traditional method that Potenlini is carried out lixiviate in operating process, Potenlini is extracted from liquorice root, then Potenlini vat liquor heating evaporation is concentrated into the 1/3-1/5 volume, concentrated solution is removed throw outs such as the protein that produces in the concentration process, pigment after filtration, regulating the pH value precipitates, obtain the Potenlini raw product, by the method for recrystallization, ion-exchange or adsorption-desorption repeatedly Potenlini is carried out purifying respectively at last.Use crystallization process, step complexity and yield are low; Make spent ion exchange resin and polymeric adsorbent, need the Potenlini vat liquor is carried out the purifying pre-treatment, in order to avoid stop up resin, this has not only increased process cost, also can reduce the rate of recovery of Potenlini.
Solvent extraction is mainly used in aspect such as metal ion and organic purifying in the mineral substance as a kind of traditional extraction and purification process, is also obtaining application widely aspect the purification of medicine at present.(Ceng Lu in the document, open as the heart of a lotus seed, the a kind of reed mentioned in ancient books in building. the high performance liquid chromatography of three kinds of saponin constituents is separated and assay in the Radix Glycyrrhizae. Acta Pharmaceutica Sinica .1990,26 (1) 53-58) it mainly is after adopting 95% ethanol lixiviate being come out from Radix Glycyrrhizae with Potenlini, through the Potenlini that concentrates, Acid precipitation makes crude product that solvent method extracts Potenlini; With propyl carbinol the crude product Potenlini is dissolved then, adopt 1% sodium hydrogen carbonate solution extraction, use n-butanol extraction after the alkali lye acidifying again; Reclaim propyl carbinol at last, gained Potenlini acetate recrystallization method purifying.Aforesaid method will concentrate with purifying and separate, and energy consumption is big, step is complicated, and propyl carbinol solvability in water is big, cause the loss of organic solvent, and aftertreatment causes trouble to sodium hydrogen carbonate solution.
Summary of the invention
The objective of the invention is to overcome existing extractive technique length consuming time; The concentration process energy consumption is big and can cause the hydrolysis and the oxidative deformation of Potenlini; And defect of high cost, thereby provide a kind of organic combination is carried out in microwave-assisted extraction and solvent extraction, with the organic solvent of low water solubility as extraction agent, the employing solvent extration will concentrate and purification effectively is coupled, directly Potenlini is purified out from vat liquor, the purifying technique that makes whole Potenlini fast, efficient and obtain simplifying.
The objective of the invention is to be achieved through the following technical solutions:
The method of extracting Potenlini from Radix Glycyrrhizae of the present invention comprises: with microwave-assisted extraction, organic solvent extraction and water back extraction:
1) with fresh Radix Glycyrrhizae air-dry after, be processed into granular Radix Glycyrrhizae sample, be placed in the container, after adding entry and mixing, put into microwave-assisted and extract equipment (Niu Guoguang, Pan Xuejun, Jia Guanghe, An Zhentao, Liu Huizhou " the atmospheric pressure state temperature adjustment is controlled double mode stirring microwave-assisted and extracted equipment ", utility model, application number: No.02203128.6,2002.7; Microwave-assistedextraction of glycyrrhizic acid from licorice root, Xuejun Pan, Huizhou Liu, Guanghe Jia and YounYuen Shu, Biochemical Engineering Journal, 5,173-177,2000.), carry out microwave and heat at interval, filtered while hot obtains the Potenlini vat liquor;
2) adopt organic extractant that Potenlini is purified from vat liquor: at first regulate the pH to 1.5-6.0 of vat liquor with acid, adopt neutral phosphonic extraction agent or pure kind of extractants to extract then, Potenlini is enriched in organic phase;
3) adopt water to 2) in organic phase carry out back extraction: by regulating the pH to 6.0-12.0 of the aqueous solution, obtain the aqueous phase solution of enrichment Potenlini;
4) add gac at last to the processing of decolouring of the aqueous phase solution of enrichment Potenlini; Solution after the decolouring obtains refining Potenlini after Acid precipitation, drying.
Heating power 200-700W when the microwave-assisted described in the step 1 extracts.
Acid described in the step 2 is hydrochloric acid or sulfuric acid; Described neutral phosphonic extraction agent is tributyl phosphate (TBP) or trialkyl phosphine (TRPO); Described pure kind of extractants is n-Octanol, octanol-2, nonylcarbinol, isooctyl alcohol, n-hexyl alcohol or primary isoamyl alcohol; The thinner of described extraction agent is a sherwood oil.
The invention has the advantages that:
1. using microwave-assisted to extract is to utilize microwave that the different material of polarity is carried out the selectivity heating, thereby finish heterogeneity is optionally extracted.Potenlini is a kind of weak acid, and polarity is stronger, has five hydroxyls and three carboxyls, and microwave is easy to its heating Potenlini be immersed in the water fast.Therefore microwave-assisted has the advantage that extraction rate is fast, extraction efficiency is high.
2. adopt organic extractant to make the hydrolysis and oxidative deformation that can not cause Potenlini in the concentration process and rate of recovery height.
3. by extraction and back extraction, will be stranded in organic phase or raffinate water to the not too responsive impurity component of pH, thereby reach purifying Potenlini.
4. by extraction and back extraction, not only purifying Potenlini, also concentrated simultaneously extraction agent.
5. simplify the operation, reduce cost.
Embodiment
Embodiment 1. extracts Potenlini from Radix Glycyrrhizae
With diameter be 0.5-1.5 centimetre fresh Radix Glycyrrhizae air-dry after, be processed into varigrained Radix Glycyrrhizae sample.The Radix Glycyrrhizae sample that takes by weighing 5 grams places flask, after adding 100ml water mixes, puts into microwave-assisted and extracts equipment, and microwave heating power is 700W, carries out microwave and heats at interval 4 minutes, and filtered while hot obtains the Potenlini vat liquor.The cooling back is fixed molten, adopts the HPLC method to carry out glycyrrhizic acid content analysis in the vat liquor, and the rate of recovery of calculating Potenlini by formula (1) is 80.3%.
Formula (1)
With this Potenlini vat liquor 100ml, contain the sherwood oil 20ml of 10%TRPO, join in the beaker magnetic agitation respectively.Add sulfuric acid while stirring and regulate the pH value to 3.0 of mixed solution, then mixed solution is transferred in the centrifuge tube, centrifugal 3min tells organic phase.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the extraction liquid, the rate of recovery of calculating Potenlini by formula (2) is 88.2%.
Formula (2)
This organic phase is placed a beaker, add 5ml water, with the pH value to 10.0 that 2% aqueous sodium hydroxide solution is regulated mixed solution, magnetic agitation 3min transfers to mixed solution in the centrifuge tube then, and centrifugal 3min tells water.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the strip liquor, the rate of recovery of calculating Potenlini by formula (3) is 91.4%.
Formula (3)
Water after back extraction adds sulfuric acid, and the pH value of regulating mixed solution is to 1-2, and is centrifugal, leaches throw out, and drying obtains purity and be 63.5% Potenlini goods.The precipitation percentage that calculates Potenlini by formula (4) is 99%.
Formula (4)
This exsiccant Potenlini sample is dissolved in 50ml water, and magnetic agitation limit, limit adds 2% aqueous sodium hydroxide solution and all dissolves until the Potenlini sample.Add gac, stir several minutes, the processing of decolouring, elimination gac.Magnetic agitation limit in limit adds sulfuric acid to pH to 1.5 again, leaches precipitation, and drying obtains pure product Potenlini, and purity is 80%, obviously improves.
Embodiment 2. extracts Potenlini from Radix Glycyrrhizae
With diameter be 0.5-1.5 centimetre fresh Radix Glycyrrhizae air-dry after, be processed into varigrained Radix Glycyrrhizae sample.The Radix Glycyrrhizae sample that takes by weighing 5 grams places flask, after adding 100ml water mixes, puts into microwave-assisted and extracts equipment, and microwave heating power is 500W, carries out microwave and heats at interval 6 minutes, and filtered while hot obtains the Potenlini vat liquor.The cooling back is fixed molten, adopts the HPLC method to carry out glycyrrhizic acid content analysis in the vat liquor, and the rate of recovery of calculating Potenlini by formula (1) is 70%.
With this Potenlini vat liquor 100ml, contain 50% TBP sherwood oil 100ml, join in the beaker magnetic agitation respectively.Add hydrochloric acid while stirring and regulate the pH value to 3.0 of mixed solution, then mixed solution is transferred in the centrifuge tube, centrifugal 3min tells organic phase.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the extraction liquid, the rate of recovery of calculating Potenlini by formula (2) is 80.2%.
This organic phase is placed a beaker, add 100ml water, with the pH value to 11.0 that 2% aqueous sodium hydroxide solution is regulated mixed solution, magnetic agitation 3min transfers to mixed solution in the centrifuge tube then, and centrifugal 3min tells water.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the strip liquor, the rate of recovery of calculating Potenlini by formula (3) is 91.4%.
Water after back extraction adds hydrochloric acid, and the pH value of regulating mixed solution is to 1-2, and is centrifugal, leaches throw out, and drying obtains purity and be 63.5% Potenlini goods.The precipitation percentage that calculates Potenlini by formula (4) is 97%.
This exsiccant Potenlini sample is dissolved in 50ml water, and magnetic agitation limit, limit adds 2% aqueous sodium hydroxide solution and all dissolves until the Potenlini sample.Add gac, stir several minutes, the processing of decolouring, elimination gac.Magnetic agitation limit in limit adds sulfuric acid to pH to 1.5 again, leaches precipitation, and drying obtains pure product Potenlini, and purity is 77%, obviously improves.
Embodiment 3. extracts Potenlini from Radix Glycyrrhizae
With diameter be 0.5-1.5 centimetre fresh Radix Glycyrrhizae air-dry after, be processed into varigrained Radix Glycyrrhizae sample.The Radix Glycyrrhizae sample that takes by weighing 5 grams places flask, after adding 100ml water mixes, puts into microwave-assisted and extracts equipment, and microwave heating power is 200W, carries out microwave and heats at interval 10 minutes, and filtered while hot obtains the Potenlini vat liquor.The cooling back is fixed molten, adopts the HPLC method to carry out glycyrrhizic acid content analysis in the vat liquor, and the rate of recovery of calculating Potenlini by formula (1) is 65%.
With this Potenlini vat liquor 100ml, n-hexyl alcohol 50ml joins in the beaker magnetic agitation respectively.Add hydrochloric acid while stirring and regulate the pH value to 2.5 of mixed solution, then mixed solution is transferred in the centrifuge tube, centrifugal 3min tells organic phase.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the extraction liquid, the rate of recovery of calculating Potenlini by formula (2) is 88.2%.
This organic phase is placed a beaker, add 50ml water, with the pH value to 10.0 that 2% aqueous sodium hydroxide solution is regulated mixed solution, magnetic agitation 3min transfers to mixed solution in the centrifuge tube then, and centrifugal 3min tells water.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the strip liquor, the rate of recovery of calculating Potenlini by formula (3) is 91.4%.
Water after back extraction adds hydrochloric acid, and the pH value of regulating mixed solution is to 1-2, and is centrifugal, leaches throw out, and drying obtains purity and be 63.5% Potenlini goods.The precipitation percentage that calculates Potenlini by formula (4) is 98%.
This exsiccant Potenlini sample is dissolved in 50ml water, and magnetic agitation limit, limit adds 2% aqueous sodium hydroxide solution and all dissolves until the Potenlini sample.Add gac, stir several minutes, the processing of decolouring, elimination gac.Magnetic agitation limit in limit adds sulfuric acid to pH to 1.5 again, leaches precipitation, and drying obtains pure product Potenlini, and purity is 75%, obviously improves.
The time was to the influence of extraction yield during embodiment 4. microwave-assisted extracted
Taking by weighing 5g thickness is the Radix Glycyrrhizae sample of 4-5 millimeter (A) or 3-4 millimeter (B), place flask respectively, add 100ml water, put into microwave-assisted extraction equipment then, microwave heating power is 700W, the predefined program listed by table 1 heats (in case bumping) at interval, Potenlini vat liquor filtered while hot after the extraction, the cooling back is fixed molten, adopts the HPLC method to carry out the glycyrrhizic acid content analysis, the rate of recovery by formula (1) calculating Potenlini the results are shown in table 1.
Table 1, microwave-assisted extraction time is to the influence of extraction yield
Microwave-assisted extraction time 12345678
(minute)
Rate of recovery % (A) 39.7 45.9 62.3 67.1 66.8 68.0 68.5 69.0
Rate of recovery % (B) 47.6 62.7 69.5 80.3 83.7 81.5 86.3 79.6
Annotate: the A-solvent is mainly water, 100ml, Radix Glycyrrhizae thickness of sample 4-5mm, 5g;
The B-solvent is mainly water, 100ml, Radix Glycyrrhizae thickness of sample 3-4mm, 5g.
Microwave-assisted extracts and has higher extraction rate as can be seen from Table 1, microwave heating 4 minutes, and Potenlini almost reaches balance between Radix Glycyrrhizae and solvent.Prolong microwave heating time, the rate of recovery does not have change significantly.And with traditional thermal backflow extract phase ratio, latter's single extracted palpus time spent 1-2 hour, and the rate of recovery is 60-80%, and the former has obviously saved the time.
Embodiment 5. different organic solvents are to the influence of the effect of extracting of Potenlini
With step 2 of the present invention) in the Potenlini vat liquor that obtains or the commercially available pure product Potenlini aqueous solution place beaker, magnetic agitation 0.5～5 minute with different types of organic solvent (listed) of 20～100% times of volumes respectively as table 2.Regulate pH value to 1.7～5.5 of mixed solution in the whipping process.Mixed solution after the extraction is transferred in the centrifuge tube, and centrifugal 1 minute or standing demix are told organic phase.Adopt the HPLC method to carry out glycyrrhizic acid content analysis in the organic phase, calculate the percentage extraction of this solvent, the results are shown in table 2 by formula (2).
Table 2 different organic solvents is to the effect of extracting of Potenlini
Solvent pH churning time, min water/organic phase (v/v) percentage extraction %
Isooctyl alcohol 1.9 51 98.7
Isooctyl alcohol 2.0 52 92.5
The sherwood oil 2.0 52 99.0 that contains 80% n-hexyl alcohol
The sherwood oil 1.7 53 92.8 that contains 80% isooctyl alcohol
The sherwood oil 2.0 51 99.8 that contains 60%TBP
The sherwood oil 2.1 54 79.8 that contains 60%TBP
The sherwood oil 4.0 51 97.1 that contains 60%TBP
The sherwood oil 2.0 31 98.7 that contains 10%TRPO
The sherwood oil 5.5 31 80.0 that contains 10%TRPO
The sherwood oil 3.0 35 88.2 that contains 10%TRPO
The sherwood oil 3.0 0.5 1 55.6 that contains 6%TRPO
The sherwood oil 3.0 11 83.2 that contains 6%TRPO
The sherwood oil 3.0 51 89.2 that contains 6%TRPO
The sherwood oil that contains 40% n-hexyl alcohol
*2.0 51 95.2
The sherwood oil that contains 40% n-Octanol
*2.0 51 77.1
The sherwood oil that contains 40% isooctyl alcohol
*2.0 51 17.6
The sherwood oil that contains 40% octanol-2
*2.0 51 32.6
The sherwood oil that contains 40% nonylcarbinol
*2.0 51 47.0
Annotate:
*For the pure product Potenlini aqueous solution is extracted.
As can be seen from Table 2, under acidic conditions (pH is 1～5.5), Potenlini mainly exists with molecularity, and organic solvent all can extract Potenlini from the aqueous solution fast, can reach extraction equilibrium in 0.5-5 minute.To the extracting power of Potenlini from high to low put in order for: neutral phosphonic extraction agent (TRPO, TBP)＞pure kind of extractants, wherein the TRPO extracting power is greater than TBP.For pure kind of extractants, steric effect is very obvious to the extraction influence of Potenlini: along with the increase of chain length, the percentage extraction of Potenlini reduces gradually; Side chain is big more, and percentage extraction is more little.In addition, by adopting the different organic solvents of comparing, can concentrate the Potenlini vat liquor.
The effect of embodiment 6. back extraction Potenlini from the different organic solvents that is rich in Potenlini
The organic phase solution that is rich in Potenlini that obtains in the step 3) of the present invention and the water of 25～100% times of volumes are placed beaker, magnetic agitation 0.5～5 minute.Regulate pH value to 6.0～12.0 of mixed solution in the whipping process.Mixed solution after the extraction is transferred in the centrifuge tube, and centrifugal 1 minute or standing demix divide water-yielding stratum.Employing HPLC method is carried out the glycyrrhizic acid content analysis in the water layer, calculates the back extraction ratio of water to this solvent by formula (3), the results are shown in table 3.
Table 3 water is from the effect of the organic solvent back extraction Potenlini that is rich in Potenlini
Solvent pH churning time, min organic phase/water (v/v) percentage extraction %
Isooctyl alcohol 7.0 51 89.9
Isooctyl alcohol 9.7 51 89.1
The sherwood oil 6.1 51 89.7 that contains 80% n-hexyl alcohol
The sherwood oil 9.7 51 97.1 that contains 80% isooctyl alcohol
The sherwood oil 7.3 51 97.7 that contains 60%TBP
The sherwood oil 10.1 51 96.2 that contains 60%TBP
The sherwood oil 12.0 51 97.0 that contains 60%TBP
The sherwood oil 10.0 31 93.2 that contains 10%TRPO
The sherwood oil 10.0 32 92.3 that contains 10%TRPO
The sherwood oil 10.0 34 91.4 that contains 10%TRPO
The sherwood oil that contains 6%TRPO
*11.0 0.5 1 68.2
The sherwood oil that contains 6%TRPO
*11.0 11 70.4
The sherwood oil that contains 6%TRPO
*11.0 31 96.8
The sherwood oil that contains 6%TRPO
*11.0 51 100.0
Annotate:
*The Potenlini that contains in the organic solvent is pure product Potenlini.
As can be seen from Table 3, in pH6～12, the aqueous solution all can extract Potenlini from organic solvent fast, can reach extraction equilibrium in 0.5-5 minute.And by back extraction, liquor capacity reduces, and concentrates effectively.
Claims (6)
1. method of from Radix Glycyrrhizae, extracting Potenlini, comprising: microwave-assisted extraction, organic solvent extraction and water back extraction is characterized in that step is as follows:
1) with fresh Radix Glycyrrhizae air-dry after, be processed into granular Radix Glycyrrhizae sample, be placed in the container, after adding entry and mixing, put into microwave-assisted extraction equipment, carry out microwave and heat at interval, filtered while hot obtains the Potenlini vat liquor;
2) adopt organic extractant that Potenlini is purified from vat liquor: at first regulate the pH to 1.5-6.0 of vat liquor with acid, adopt neutral phosphonic extraction agent or pure kind of extractants to extract then, Potenlini is enriched in organic phase;
3) adopt water to 2) in organic phase carry out back extraction: by regulating the pH to 6.0-12.0 of the aqueous solution, obtain the aqueous phase solution of enrichment Potenlini;
4) add gac at last to the processing of decolouring of the aqueous phase solution of enrichment Potenlini; Solution after the decolouring obtains refining Potenlini after Acid precipitation, drying.
2. by the method for extracting Potenlini from Radix Glycyrrhizae described in the claim 1, it is characterized in that: the heating power when the step 1) microwave-assisted extracts is 200-700W.
3. by the method for from Radix Glycyrrhizae, extracting Potenlini described in the claim 1, it is characterized in that: step 2) described in the acid used of adjusting pH be hydrochloric acid or sulfuric acid.
4. by the method for from Radix Glycyrrhizae, extracting Potenlini described in the claim 1, it is characterized in that: step 2) described in the neutral phosphonic extraction agent be tributyl phosphate or trialkyl phosphine.
5. by the method for from Radix Glycyrrhizae, extracting Potenlini described in the claim 1, it is characterized in that: step 2) described in pure kind of extractants be n-Octanol, octanol-2, nonylcarbinol, isooctyl alcohol, n-hexyl alcohol or primary isoamyl alcohol.
6. by the method for extracting Potenlini from Radix Glycyrrhizae described in the claim 1,4 or 5, it is characterized in that: the thinner of described extraction agent is a sherwood oil.
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| CN100343205C (en) * | 2005-11-15 | 2007-10-17 | 江西汇仁药业有限公司 | Method for separating Chinese medicinal active substances |
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| CN100334446C (en) * | 2005-11-09 | 2007-08-29 | 中国科学院长春应用化学研究所 | Method for detecting constituents of liquorice-ginseng capsule |
| CN101067146B (en) * | 2007-04-25 | 2011-07-27 | 重庆立克生物技术有限公司 | Biological extraction process of glycyrrhizic acid |
| CN102304165B (en) * | 2011-07-12 | 2012-12-05 | 北京服装学院 | Method for extracting and purifying glycyrrhizic acid by ion-exchange fibers |
| CN104004047A (en) * | 2014-05-27 | 2014-08-27 | 普凡生生物科技(北京)有限公司 | Preparation method of glycyrrhetinic acid |
| CN104004046A (en) * | 2014-05-27 | 2014-08-27 | 普凡生生物科技(北京)有限公司 | Preparation method of acetyl glycyrrhetinic acid |
| CN104083431A (en) * | 2014-07-17 | 2014-10-08 | 新疆鹏弘生物科技有限公司 | Comprehensive liquorice extraction process |
| CN105669821B (en) * | 2016-01-08 | 2017-05-10 | 中国农业大学 | Preparation method of glycyrrhetinic acid monoglucuronide |
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| WO2021134407A1 (en) * | 2019-12-31 | 2021-07-08 | 中国医药健康产业股份有限公司 | Method for extracting crude product of glycyrrhizic acid and glycyrrhizic flavone from licorice and purifying to obtain glycyrrhizinate |
| CN115624576B (en) * | 2022-10-21 | 2024-04-02 | 劲牌有限公司 | Preparation and decoloring method of licorice extract |
| CN117959755B (en) * | 2023-07-03 | 2024-08-30 | 太和康美(北京)中医研究院有限公司 | Directional enrichment method of flavonoid compounds and directional enrichment method thereof |
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| CN100343205C (en) * | 2005-11-15 | 2007-10-17 | 江西汇仁药业有限公司 | Method for separating Chinese medicinal active substances |
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