CN1201697A - Preparation of human amnion - Google Patents
Preparation of human amnion Download PDFInfo
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- CN1201697A CN1201697A CN 97111074 CN97111074A CN1201697A CN 1201697 A CN1201697 A CN 1201697A CN 97111074 CN97111074 CN 97111074 CN 97111074 A CN97111074 A CN 97111074A CN 1201697 A CN1201697 A CN 1201697A
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- amniotic membrane
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Abstract
A process for preparing human amnion used for surgical operation includes such technological steps as collecting fetal membranes after normal delivery, separating the layer of fetal membrane facing against fetus from the layer containing blood vessel to obtain amnion, washing it with physiological saline, immersing it in 0.1% antamon solution for 3-5 min, putting it in aseptic container full of physiological saline, freeze drying, putting in double-layer plastic bag and radiating it with cobalt-60. The obtained human amnion features no obvious inflammation and exclusion reactions for wound, better healing and no adhering to muscle tendon.
Description
The present invention discloses and a kind ofly is applied to surgical operation, especially prevents the preparation method of people's amniotic membrane of using in the tendon rupture repairing operation of adhesion of tendon.
The identical posterior synechiae of tendon injury is to influence one of key factor of hands, sufficient functional rehabilitation, at present, still the research of not having the way of the way control adhesion of tendon that well solves adhesion of tendon and material clinically is the important topic in the current surgical field, traditional restorative procedure and material, such as control adhesions of tendon such as casing, macromolecule material products, its effect is all desirable not to the utmost.
In recent years, along with the development of microtechnique and experimentation, discovery also confirms that tendon is to have tremulous pulse, venous circulation, and lymphatic return, the synovial fluid exchange mechanism has disclosed tendon and has had inherent healing ability, and do not relied on cell and adhesion outside the tendon.This important breakthrough is found to be the control adhesion of tendon, recovers limb function and more in depth study to have opened a new world out-of-the-way, and the tendon sheath no longer is the principal element that influences healing process of tendons.First phase was repaired effective behave that stndon sheath is considered to prevent adhesion of tendon when kiss connect tendon, and medicine and physical therapy, and early stage controlled activity has also had further understanding to the influence of healing process of tendons and adhesion of tendon.But stndon sheath is damaged, and there is coarse union of fracture section simultaneously in the healing process of tendons place, and the tenolysis postoperative of being expert at forms adhesion very easily once again.How to prevent this type of adhesion or loosen after again the generation of adhesion still get nowhere.Domestic and international casing, macromolecule material product such as the pellosil that adopts, polytetrafluoroethyl-ne acyl film, its effect is satisfied not to the utmost, such as rejection, infects etc., and the second operation that also needs that has takes out isolating membrane, has increased patient's misery and financial burden.
The object of the present invention is to provide a kind of preparation method that can be applicable to the people's amniotic membrane in the surgical operation.
Technical scheme of the present invention is:
Get the fetal membrane of normal labor, under the aseptic condition, the fetus face of fetal membrane is separated from the centre with the sub-official's face that contains blood vessel, keep the fetus face, flush away blood in normal saline, at this moment amniotic membrane (fetus face) be creamy white, uniform and smooth, translucent thin film, after 1 ‰ geramine liquid soak 3～5 fens kinds, insert one and fill in the sterile chamber of normal saline, it is standby to put into refrigerator, through the amniotic membrane of handling like this, join foster 72 hours asepsis growths as antibacterial.
Freeze dried people's amniotic membrane (shaping membrane): this film is white in color or is little faint yellow, translucent, as bamboo paper, encloses in the two layers of plastic bag, after disinfecting with the co-60 radiation method (exposure dose 0.5Mrad, 1Mrad, 1.5Mrad, 2.0Mrad[Megarad] standby.Cultivate 7 days no bacterial growths through people's amniotic membrane that the method was handled.
People's amniotic membrane implantation prevention adhesion of tendon and clinical practice result with method for preparing show, people's amniotic membrane can be used as a kind of new adherence preventing material and is applied to clinical when the tendon that ruptures is repaired, can play prevention adhesion of tendon effect at the peripheral people's amniotic membrane of implanting of repairing section tendon, the recovery of favourable operation back limb function, reduce the maimed person of limbs, shorten treatment time
As allochthonous biomembrane, people's amniotic membrane has following advantage and characteristics: 1. material source is easy, and almost delivery room every day has fetal membrane to provide; 2. disperse is picked up from the nutrition of amniotic membrane itself, is suitable for free grafting, and its permeability does not influence the nutrition supply of tendon; 3. slight, the local a small amount of application of immunoreation does not produce tangible inflammatory reaction or rejection; 4. absorbability is good, does not need second operation to take out.In view of above advantage, at first carry out the animal experiment study that people's amniotic membrane is implanted the prevention adhesion of tendon, on zooperal basis, carry out the transition to the clinical practice that adhesion of tendon is repaired in prevention.
One. zoopery part: carry out twice
From in February, 95 to June, in Changfeng County Yang Miao town plant, select when 50 of real estate broiler (AA kind), body weight 2.0～2.5kg, male and female are regardless of, and 5 one group, are divided into 10 groups.If experimental group, matched group, blank group.Postoperative experimental chicken is still raised by the chicken farm poultry raiser is unified.1 week of postoperative, 2 weeks, 3 weeks according to plan ... 5 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks, 16 weeks, criticize by component and to take specimen, do big soma, pathology light microscopic and electron microscopic observation.Because sheep chicken house condition is relatively poor, the chicken of raising is more, and fowl plague took place again at the beginning of 4 months, and experimental chicken is also followed large quantities of death.Because of the minimizing of experimental chicken quantity, the basic failure of experiment for the first time.But from the experimental chicken preliminary observation result who survives, people's amniotic membrane still can seen after 2 weeks around the implantation tendon, and 3 Zhou Houyi debate not unclear.The tendon of implanting the amniotic membrane experimental group is than matched group, and the tendon periphery of blank group is smooth, and adhesion is less.
2. at the beginning of 96 years 4 months, carried out the 2nd zoopery from December, 95 first arrival in No.1 Reople's Hospital, Hefei City.Get identical (AA kind) tame 30 chickens, body weight 2.0～2.5kg male and female are regardless of, and five one group, be divided into 6 groups, numbering is divided cage, and the special messenger raises.If experimental group, matched group, blank group.Taked specimen in 3 months, 4 months.Do (1) naked eyes specimen observation substantially (all specimen) respectively; (2) tissue slice om observation (totally 24 chickens, 48 specimen); (3) scanning electron microscopic observation (totally 6 chickens, 12 specimen).The result shows: the human amnion tissue compatibility in tendon week is good, and rejection is slight, and tendon in the reparation and surrounding tissue do not have obvious adhesion, and regression, absorption appear in the visible amniotic membrane of specimen in 3,4 weeks of postoperative, forms the crack in tendon week, helps the tendon motion.
Two. on last motion thing experiment basis, carry out clinical practice
On the basis that preliminary observation experiment tendon surrounding tissue changes, beginning is a small amount of, clinical practice selectively, therapeutic effect is satisfied, postoperative wound does not have obvious inflammatory reaction, the rejection phenomenon do not occur, and wound healing is good, the identical tendon tension force of postoperative 2,3 weekly checks is normal, and tendon did not have obvious adhesion when patient initiatively did to stretch in the wrong the action.
Three. the preparation method of people's amniotic membrane
＜one〉gets fresh eutocous fetal membrane, under aseptic condition, separate from the centre with the uterus face that contains blood vessel by vascular forceps and the Small clamp fetus face with fetal membrane, uterus face fetal membrane discards, clean blood on the fetus face fetal membrane repeatedly with normal saline after, obtain promptly that fetus face fetal membrane---people amniotic membrane is creamy white, uniform and smooth, translucent thin film is after soaking 3 minutes with 1 ‰ geramine liquid, insert one and fill in the disinfecting container of normal saline, it is standby to put into refrigerator.Through the amniotic membrane of handling like this, make 72 hours asepsis growths of antibacterial culturing.
＜two〉take one of people's amniotic membrane that said method handled, its size cuts randomly, after from normal saline, taking out, paste immediately and be layered on the clean sheet glass, put into a frost-free refrigerator-freezer cold preservation, about two all left and right times, but people's amniotic membrane lyophilizing, take out the back from the people's amniotic membrane of tearing on glass, this moment the people amniotic membrane be white in color or little faint yellow, translucent, as bamboo paper, enclose in the two layers of plastic bag, disinfect (dosage 1.6Mrad) amniotic membrane after disinfecting, cultivate 7 days asepsis growths with the co-60 radiation method.
Claims (3)
1. the preparation method of people's amniotic membrane, it comprises:
A. get eutocous fetal membrane, under aseptic condition, the fetus face and the sub-official's face that contains blood vessel of fetal membrane separated from the centre, keep fetus face fetal membrane;
B. above-mentioned amniotic membrane is used normal saline flush away blood, amniotic membrane is creamy white, and is uniform and smooth, translucent thin film;
C. after soaking 3～5 fens kinds with 1 ‰ geramine liquid, insert one and fill in the sterile chamber of normal saline and be placed in the refrigerator standby.
2. the preparation method of people's amniotic membrane according to claim 1, it also comprises people's amniotic membrane that said method is made, freeze in after be white in color or little faint yellow, translucent, by being enclosed in the two layers of plastic bag, disinfect afterwards with the co-60 radiation method and to store.
3. the preparation method of people's amniotic membrane according to claim 2, it comprises: the exposure dose of handling with co-60 radiation is: 0.5Mrad, 1.0Mrad, 1.5Mrad, 2.0Mrad, 2.5Mrad[Megarad].
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97111074 CN1201697A (en) | 1997-06-07 | 1997-06-07 | Preparation of human amnion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97111074 CN1201697A (en) | 1997-06-07 | 1997-06-07 | Preparation of human amnion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1201697A true CN1201697A (en) | 1998-12-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97111074 Pending CN1201697A (en) | 1997-06-07 | 1997-06-07 | Preparation of human amnion |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001008716A1 (en) * | 1999-08-02 | 2001-02-08 | Ann Pinfold | Method of preservation of amnion and uses thereof |
| WO2002079456A3 (en) * | 2001-03-30 | 2003-10-23 | Maria Ramos | A method for the preparation of immunologically inert amniotic membranes |
| CN103074295A (en) * | 2013-02-06 | 2013-05-01 | 施萍 | Construction method of medical human amniotic membrane tissue reserve bank |
| CN103356703B (en) * | 2005-03-31 | 2016-04-06 | 斯丹姆涅恩有限公司 | Prepare the method for the medicine in order to treat chronic infection wound |
| CN112584878A (en) * | 2018-08-30 | 2021-03-30 | 樱花精机株式会社 | Regeneration and repair material for tendon and tendon sheath and application of repair material |
-
1997
- 1997-06-07 CN CN 97111074 patent/CN1201697A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001008716A1 (en) * | 1999-08-02 | 2001-02-08 | Ann Pinfold | Method of preservation of amnion and uses thereof |
| WO2002079456A3 (en) * | 2001-03-30 | 2003-10-23 | Maria Ramos | A method for the preparation of immunologically inert amniotic membranes |
| CN103356703B (en) * | 2005-03-31 | 2016-04-06 | 斯丹姆涅恩有限公司 | Prepare the method for the medicine in order to treat chronic infection wound |
| CN103074295A (en) * | 2013-02-06 | 2013-05-01 | 施萍 | Construction method of medical human amniotic membrane tissue reserve bank |
| CN103074295B (en) * | 2013-02-06 | 2014-10-08 | 施萍 | Construction method of medical human amniotic membrane tissue reserve bank |
| CN112584878A (en) * | 2018-08-30 | 2021-03-30 | 樱花精机株式会社 | Regeneration and repair material for tendon and tendon sheath and application of repair material |
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