CN119791109A - Application of natural unsaturated fatty aldehydes in controlling storage pests - Google Patents
Application of natural unsaturated fatty aldehydes in controlling storage pests Download PDFInfo
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Abstract
本发明公开了天然不饱和脂肪醛在防治仓储害虫中的应用。该天然不饱和脂肪醛为反,反‑2,4‑庚二烯醛和反‑2‑庚烯醛。所述仓储害虫为包括米象、玉米象、绿豆象、咖啡豆象、锯谷盗、拟谷盗、大谷盗、谷蠹、斑皮蠹、烟草甲、小圆皮蠹、黑皮蠹和黑菌虫等的鞘翅目仓储害虫及以米蛾、麦蛾、印度谷螟、粉斑螟和地中海螟等的鳞翅目仓储害虫。本发明首次公开了反,反‑2,4‑庚二烯醛和反‑2‑庚烯醛能够通过熏蒸方式表现出优异的仓储害虫灭杀活性，为开发绿色环保杀虫剂提供了依据，具有替代传统化学杀虫剂的良好应用前景。The invention discloses the application of natural unsaturated fatty aldehydes in preventing and controlling storage pests. The natural unsaturated fatty aldehydes are trans, trans-2,4-heptadienal and trans-2-heptenal. The storage pests are coleopteran storage pests including rice weevils, corn weevils, mung bean weevils, coffee bean weevils, saw-saw grain beetles, flour beetles, large grain beetles, grain borers, scaly beetles, tobacco beetles, small round-skinned beetles, black-skinned beetles and black fungus borers, and lepidopteran storage pests including rice moths, wheat moths, Indian meal borers, pink moths and Mediterranean borers. The invention discloses for the first time that trans, trans-2,4-heptadienal and trans-2-heptenal can show excellent storage pest killing activity by fumigation, provides a basis for the development of green and environmentally friendly pesticides, and has a good application prospect of replacing traditional chemical pesticides.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to application of natural unsaturated fatty aldehyde in controlling storage pests.
Background
The stored pests form a great threat to grain quality and storage safety, resulting in grain quality degradation and a great deal of loss. According to the investigation of the grain and agriculture organization of the united nations, the losses caused by stored grain pests are about 4% to 8% of the total storage, which in some countries may even be as high as 30%. Therefore, how to scientifically and effectively prevent and control grain storage pests and protect stored grains from pests becomes a challenge to be solved in the field of grain storage safety.
At present, the low-temperature grain storage technology and the air-conditioning insecticidal technology are commonly applied to pest control of grain storage. However, physical control of low temperature and nitrogen-filled regulation (hypoxia) still faces pest occurrence problems, and low temperature-resistant and hypoxia-resistant pests are difficult to thoroughly remove. The application scene of the nitrogen regulating technology is mainly a grain storage environment in the south, and the nitrogen regulating technology has the defects of long operation period, high requirement on environment tightness and the like. Chemical fumigants have great effectiveness in pest control applications in grain storage, such as sulfuryl fluoride and aluminum phosphide fumigation techniques. However, there are studies showing that sulfuryl fluoride is a powerful greenhouse gas, which has a non-negligible effect on global warming. The long-term use of aluminum phosphide also leads to the enhancement of pest resistance, and is difficult to achieve the effect of efficiently killing pests, and in addition, a large amount of highly toxic gas can be generated when the aluminum phosphide is decomposed, so that the harm is caused to the health of human bodies. Chemical control is cheap and efficient, but can harm grain safety, damage environment and influence human and animal health. Therefore, there is an urgent need to develop new efficient, environmentally friendly pest control technologies for stored grain to supplement the shortcomings of the existing control technologies.
Naturally unsaturated fatty aldehydes are a class of compounds commonly found in volatile essential oils of plants, which play an important role in plant defense mechanisms against pathogenic microorganisms. These compounds not only emit fragrance, but also have important value as natural perfume resources. In particular, trans-2, 4-heptadienal and trans-2-heptenal are two typical plant synthesized natural unsaturated fatty aldehydes, which have been certified as safe flavorants and preservatives by the food additive use Standard (GB 2760) and the United states Food and Drug Administration (FDA) published by the national health Commission. The efficacy of the two natural unsaturated fatty aldehydes in grain storage pest control is evaluated in the study. The result shows that the trans, trans-2, 4-heptadienal and trans-2-heptenal can effectively kill grain storage pests through fumigation treatment, thereby exhibiting potential as a novel pesticide. In view of its high efficiency and safety, trans-2, 4-heptadienal and trans-2-heptenal have further research and development value in the field of pest control in grain storage silos.
Disclosure of Invention
In view of the above, the present invention aims to provide the use of natural unsaturated fatty aldehydes for controlling storage pests. Wherein the natural unsaturated fatty aldehyde comprises any one of trans, trans-2, 4-heptadienal and trans-2-heptenal. The natural unsaturated fatty aldehyde has strong volatility in a natural state and can be directly used as a storage pest fumigant.
Preferably, the naturally unsaturated fatty aldehyde is trans, trans-2, 4-heptadienal and/or trans-2-heptenal.
Preferably, the stored pests include coleopteran stored pests such as rice weevil, corn weevil, green bean weevil, coffee bean weevil, saw coryza sativa, anthropomorphic coryza sativa, codling, black bark beetle, and black fungus, and lepidopteran stored pests such as rice moth, wheat moth, indian meal moth, pink bollworm, and mediterranean moth.
Preferably, the natural unsaturated fatty aldehyde is used for controlling storage pests in a fumigation mode.
Preferably, the concentration of natural unsaturated fatty aldehyde used for fumigation is 1.5-36 mu L/L.
Preferably, the fumigation is performed with a trans, trans-2, 4-heptadienal concentration of 20. Mu.L/L and a trans-2-heptenal concentration of 36. Mu.L/L.
The natural unsaturated fatty aldehyde of the invention is trans, trans-2, 4-heptadienal and trans-2-heptenal have broad spectrum killing effect on storage pests, and can be used for controlling coleoptera storage pests such as rice weevil, corn weevil, green bean weevil, coffee bean weevil, saw, amoebola, larch, bark beetle, black fungus and the like, and lepidoptera storage pests such as rice moth, wheat moth, indian meal moth, pink bollworm, mediterranean moth and the like. Natural unsaturated fatty aldehyde widely exists in plants, is green and environment-friendly, has strong volatility and can kill storage pests through fumigation action. The trans-2, 4-heptadienal and the trans-2-heptenal have excellent activity of killing storage pests, can replace the traditional chemical storage pest killing agent, and have wide application prospect in the field of pest control.
Detailed Description
The present invention will be described in detail by the following examples, which are intended to demonstrate the effect of the present invention and are not to be construed as representing the limiting scope of the present invention. The scope of the present invention is not limited to the following embodiments or examples, and any modifications and substitutions made in accordance with the present invention are intended to be included in the scope of the present invention. In the following examples, unless otherwise indicated, all experimental reagents used were commercially available. The natural unsaturated fatty aldehydes used in the following examples were trans, trans-2, 4-heptadienal and trans-2-heptenal were purchased from Shanghai Ala Biotechnology Co., ltd.
Example 1 control of the storage Rice pests, the bark beetles, by Natural unsaturated fatty aldehydes
1) Test pest
The rice-flour beetle is derived from rice.
2) Fumigation agent
Trans-2, 4-heptadienal was fumigated at concentrations of 1.5. Mu.L/L, 3. Mu.L/L and 6. Mu.L/L, respectively, and trans-2-heptenal was fumigated at concentrations of 6. Mu.L/L, 12. Mu.L/L and 18. Mu.L/L, respectively. Note that 6. Mu.L/L refers to the use of 6. Mu.L of a stock solution of trans, trans-2, 4-heptadienal or trans-2-heptenal per cubic decimeter of space, and the rest are similar.
3) Fumigating test
About 50 adult rice beetles were placed in a 1L square acrylic box (i.e., 1 cubic centimeter of space volume), respectively, and a well slide was placed in the box, and the fumigant concentrations in 2) were applied as independent treatment groups, respectively, with an equivalent ddH 2 O as a control, and each treatment was repeated three times. And sealing by using a PARAFILM film after covering the square acrylic box. Then, the mixture was placed in a 28 ℃ incubator for 24 hours, and the corrected mortality was calculated according to the following formula:
Corrected mortality = (treatment mortality-control mortality)/(1-control mortality) ×100%.
4) Analysis of results
The 24-hour fumigation activity of the trans-2, 4-heptadienal and the trans-2-heptenal on the end of the bark beetle is shown in Table 1,6 mu L/L of trans, and the corrected death rate of the trans-2, 4-heptadienal or 18 mu L/L of trans-2-heptenal on the end of the bark beetle is 100%, which indicates that the natural unsaturated fatty aldehyde has excellent fumigation activity on the end of the bark beetle at low dosage.
TABLE 1 fumigating Activity of natural unsaturated fatty aldehyde on bark beetle adults within 24h
Example 2 control of storage mung bean pests, green bean weevil, by Natural unsaturated fatty aldehyde
1) Test pest
The green bean image is derived from mung bean.
2) Fumigation agent
Trans-2, 4-heptadienal was fumigated at concentrations of 1.5. Mu.L/L, 3. Mu.L/L and 6. Mu.L/L, respectively, and trans-2-heptenal was fumigated at concentrations of 3. Mu.L/L, 6. Mu.L/L and 12. Mu.L/L, respectively.
3) Fumigating test
About 50 green bean image adults were placed in 1L square acrylic boxes (i.e., 1 cubic centimeter of space volume), respectively, concave hole slides were placed in the boxes, and the fumigant concentrations in 2) were applied as independent treatment groups, respectively, with equal amounts of ddH 2 O as controls, and each treatment was repeated three times. And sealing by using a PARAFILM film after covering the square acrylic box. Then, the mixture was placed in a 22 ℃ incubator and treated for 24 hours, and a corrected mortality was calculated, and the corrected mortality calculation formula was the same as in example 1.
4) Analysis of results
The 24-hour fumigation activity of the trans-2, 4-heptadienal and the trans-2-heptenal on the green bean image adults with different concentrations is shown in Table 2,6 mu L/L of trans, and the corrected death rate of the trans-2, 4-heptadienal or 12 mu L/L of trans-2-heptenal on the green bean image adults is 100%, which indicates that the natural unsaturated fatty aldehyde has excellent fumigation activity on the green bean image adults under low dosage.
TABLE 2 fumigating Activity of Natural unsaturated fatty aldehyde on Bulbophyllum inophyllum for 24h
Example 3 control of storage wheat pests on anthropomorphic theft by Natural unsaturated fatty aldehyde
1) Test pest
The anthropomorphic cereal is derived from wheat.
2) Fumigation agent
Trans-2, 4-heptadienal was fumigated at concentrations of 1.5. Mu.L/L, 3. Mu.L/L and 6. Mu.L/L, respectively, and trans-2-heptenal was fumigated at concentrations of 3. Mu.L/L, 6. Mu.L/L and 12. Mu.L/L, respectively.
3) Fumigating test
About 50 adult anthropomorphic insects were placed in a 1L square acrylic box (i.e., 1 cubic centimeter of space volume), a concave slide was placed in the box, and the fumigant concentrations in 2) were applied as independent treatment groups, each treatment was repeated three times with an equivalent ddH 2 O as a control. And sealing by using a PARAFILM film after covering the square acrylic box. Then, the mixture was placed in a 28 ℃ incubator and treated for 24 hours, and a corrected mortality was calculated, and the corrected mortality calculation formula was the same as in example 1.
4) Analysis of results
The 24-hour fumigation activity of the trans, trans-2, 4-heptadienal and the trans-2-heptenal with different concentrations on the adult botrytis cinerea is shown in Table 3,6 mu L/L trans, and the corrected death rate of the trans-2, 4-heptadienal or the trans-2-heptenal with the concentration of 12 mu L/L is 100%, which shows that the natural unsaturated fatty aldehyde has excellent fumigation activity on the adult botrytis cinerea under low dosage.
TABLE 3 fumigating Activity of Natural unsaturated fatty aldehyde on adult Paeonia cerealis within 24h
Example 4 control of stored Rice insect Mixiang by Natural unsaturated fatty aldehyde
1) Test pest
The rice is derived from rice.
2) Fumigation agent
Trans-2, 4-heptadienal was fumigated at concentrations of 3. Mu.L/L, 6. Mu.L/L and 12. Mu.L/L, respectively, and trans-2-heptenal was fumigated at concentrations of 12. Mu.L/L, 24. Mu.L/L and 36. Mu.L/L, respectively.
3) Fumigating test
About 50 beige imago were placed in 1L square acryl boxes (i.e., 1 cubic decimeter of space volume), respectively, concave hole slides were placed in the boxes, and the concentration of the fumigant in 2) was applied as independent treatment groups, respectively, with an equal amount of ddH 2 O as a control, and each treatment was repeated three times. And sealing by using a PARAFILM film after covering the square acrylic box. Then, the mixture was placed in a 26 ℃ incubator and treated for 24 hours, and a corrected mortality was calculated, and the corrected mortality calculation formula was the same as in example 1.
4) Analysis of results
The 24-hour fumigation activity of trans-2, 4-heptadienal and trans-2-heptenal on rice weevil adults with different concentrations is shown in Table 4,12 mu L/L of trans, and the corrected death rate of trans-2, 4-heptadienal or 36 mu L/L of trans-2-heptenal on rice weevil adults is 100%, which indicates that the natural unsaturated fatty aldehyde has excellent fumigation activity on rice weevil adults with low dosage.
TABLE 4 fumigating Activity of naturally unsaturated fatty aldehyde on Miq imago within 24h
Example 5 control of stored rice pests, mimoth, by Natural unsaturated fatty aldehyde
1) Test pest
The rice moth is derived from rice.
2) Fumigation agent
The fumigating test was carried out on trans-2, 4-heptadienal and trans-2-heptenal at concentrations of 5. Mu.L/L, 10. Mu.L/L and 20. Mu.L/L, respectively.
3) Fumigating test
About 50 rice moth larvae were placed in 1L square acrylic boxes (i.e., 1 cubic decimeter of space volume), respectively, concave hole slides were placed in the boxes, and the concentration of the fumigant in 2) was applied as independent treatment groups, respectively, with an equal amount of ddH 2 O as a control, and each treatment was repeated three times. And sealing by using a PARAFILM film after covering the square acrylic box. Then, the mixture was placed in a 26 ℃ incubator and treated for 24 hours, and a corrected mortality was calculated, and the corrected mortality calculation formula was the same as in example 1.
4) Analysis of results
The 24-hour fumigation activity of the trans-2, 4-heptadienal and the trans-2-heptenal on the rice moth larvae with different concentrations is shown in Table 5,20 mu L/L of trans, and the corrected death rate of the trans-2, 4-heptadienal or the trans-2-heptenal on the rice moth larvae is 100%, which shows that the natural unsaturated fatty aldehyde has excellent fumigation activity on the rice moth larvae at low dosage.
TABLE 5 fumigation Activity of Natural unsaturated fatty aldehyde on Mimoth larvae for 24h
In conclusion, the natural unsaturated fatty aldehyde trans, trans-2, 4-heptadienal and trans-2-heptenal have excellent fumigation activity on various storage pests, and have further research, development and utilization values in pest control.
The above examples and/or experimental examples describe in detail preferred embodiments of the present invention, however, the scope of the present invention is not limited to the specific details of the above embodiments, and various direct modifications and variations of the technical solution of the present invention are allowed within the technical framework of the present invention, and all such modifications and variations should be regarded as a part of the scope of the present invention.
Claims (8)
1. The use of a natural unsaturated fatty aldehyde for controlling storage pests, wherein the natural unsaturated fatty aldehyde comprises trans, trans-2, 4-heptadienal or trans-2-heptenal.
2. Use according to claim 1, wherein the naturally occurring unsaturated fatty aldehyde is trans, trans-2, 4-heptadienal and/or trans-2-heptenal.
3. The use of claim 1, wherein the stored pests include coleopteran and lepidopteran stored pests.
4. The use of claim 1, wherein the coleopteran storage pests include rice weevil, corn weevil, green bean weevil, coffee bean weevil, saw, anthropomorphic, larch, bark beetle, toenail, small bark beetle, black bark beetle, and black fungus worm, and the lepidopteran storage pests include rice moth, wheat moth, indian meal moth, pink moth, and mediterranean moth.
5. The use according to claim 1, wherein the natural unsaturated fatty aldehyde is used for controlling stored-goods pests by fumigation.
6. The use according to claim 4, wherein fumigation is used at a concentration of 0.1 to 1000 μl/L.
7. The use according to claim 6, wherein fumigation is used at a concentration of 1.5 to 36 μl/L.
8. The use according to claim 7, wherein said trans, trans-2, 4-heptadienal is fumigated at a concentration of 20 μl/L and said trans-2-heptenal is fumigated at a concentration of 36 μl/L.
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