CN118804974A - 多核苷酸合成的方法 - Google Patents
多核苷酸合成的方法 Download PDFInfo
- Publication number
- CN118804974A CN118804974A CN202280091507.5A CN202280091507A CN118804974A CN 118804974 A CN118804974 A CN 118804974A CN 202280091507 A CN202280091507 A CN 202280091507A CN 118804974 A CN118804974 A CN 118804974A
- Authority
- CN
- China
- Prior art keywords
- nucleotide
- polymerase
- phosphatase
- conjugate
- nucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/525—Phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163290320P | 2021-12-16 | 2021-12-16 | |
| US63/290,320 | 2021-12-16 | ||
| PCT/US2022/081871 WO2023115040A2 (en) | 2021-12-16 | 2022-12-16 | Methods of polynucleotide synthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118804974A true CN118804974A (zh) | 2024-10-18 |
Family
ID=86773663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280091507.5A Pending CN118804974A (zh) | 2021-12-16 | 2022-12-16 | 多核苷酸合成的方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20250034610A1 (https=) |
| EP (1) | EP4448739A4 (https=) |
| JP (1) | JP2024546987A (https=) |
| KR (1) | KR20240137570A (https=) |
| CN (1) | CN118804974A (https=) |
| AU (1) | AU2022416658A1 (https=) |
| CA (1) | CA3240459A1 (https=) |
| WO (1) | WO2023115040A2 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024163450A2 (en) * | 2023-01-30 | 2024-08-08 | Ansa Biotechnologies, Inc. | Methods and systems for polymer synthesis by contacting synthesis surfaces with compartmentalized liquid reagents |
| CN118852357B (zh) * | 2024-09-26 | 2025-03-18 | 青岛农业大学 | 一种抗冻肽、抗冻剂及其在食品冷冻保护中的应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017223517A1 (en) * | 2016-06-24 | 2017-12-28 | The Regents Of The University Of California | Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates |
-
2022
- 2022-12-16 KR KR1020247023624A patent/KR20240137570A/ko active Pending
- 2022-12-16 US US18/719,073 patent/US20250034610A1/en active Pending
- 2022-12-16 AU AU2022416658A patent/AU2022416658A1/en active Pending
- 2022-12-16 JP JP2024536093A patent/JP2024546987A/ja active Pending
- 2022-12-16 EP EP22908777.0A patent/EP4448739A4/en active Pending
- 2022-12-16 CA CA3240459A patent/CA3240459A1/en active Pending
- 2022-12-16 WO PCT/US2022/081871 patent/WO2023115040A2/en not_active Ceased
- 2022-12-16 CN CN202280091507.5A patent/CN118804974A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20240137570A (ko) | 2024-09-20 |
| EP4448739A2 (en) | 2024-10-23 |
| CA3240459A1 (en) | 2023-06-22 |
| WO2023115040A2 (en) | 2023-06-22 |
| US20250034610A1 (en) | 2025-01-30 |
| AU2022416658A1 (en) | 2024-07-25 |
| EP4448739A4 (en) | 2026-03-18 |
| WO2023115040A3 (en) | 2023-08-03 |
| JP2024546987A (ja) | 2024-12-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |