CN118647730A - 制备核酸用于进一步分析其序列 - Google Patents

制备核酸用于进一步分析其序列 Download PDF

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Publication number
CN118647730A
CN118647730A CN202380019938.5A CN202380019938A CN118647730A CN 118647730 A CN118647730 A CN 118647730A CN 202380019938 A CN202380019938 A CN 202380019938A CN 118647730 A CN118647730 A CN 118647730A
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CN
China
Prior art keywords
nucleic acid
sequence
target
oligonucleotide
barcode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380019938.5A
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English (en)
Chinese (zh)
Inventor
宋慧媛
丹尼斯·爱德华·普罗森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
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Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of CN118647730A publication Critical patent/CN118647730A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202380019938.5A 2022-02-07 2023-02-06 制备核酸用于进一步分析其序列 Pending CN118647730A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263307559P 2022-02-07 2022-02-07
US63/307,559 2022-02-07
PCT/US2023/062070 WO2023150763A1 (en) 2022-02-07 2023-02-06 Preparing nucleic acid for further analysis of their sequence

Publications (1)

Publication Number Publication Date
CN118647730A true CN118647730A (zh) 2024-09-13

Family

ID=85873718

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380019938.5A Pending CN118647730A (zh) 2022-02-07 2023-02-06 制备核酸用于进一步分析其序列

Country Status (5)

Country Link
US (1) US20250109427A1 (https=)
EP (1) EP4476359A1 (https=)
JP (1) JP2025506418A (https=)
CN (1) CN118647730A (https=)
WO (1) WO2023150763A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2024086167A2 (en) * 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2839031B1 (en) * 2012-04-19 2017-03-29 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7522189B2 (ja) * 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US20210238661A1 (en) 2020-01-31 2021-08-05 Becton, Dickinson And Company Mesophilic dna polymerase extension blockers
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay

Also Published As

Publication number Publication date
US20250109427A1 (en) 2025-04-03
WO2023150763A1 (en) 2023-08-10
EP4476359A1 (en) 2024-12-18
JP2025506418A (ja) 2025-03-11

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