CN118243476A - Slice dyeing device and use method - Google Patents

Abstract

The invention discloses a slice dyeing device and a use method thereof, and belongs to the technical field of slice dyeing. After application, the whole operation is simpler, the slice cleaning effect is better, the whole equipment is simple to prepare, and the method is suitable for slice dyeing experiments in laboratories and students with poor most conditions at ordinary times.

Description

Slice dyeing device and use method

Technical Field

The invention relates to the technical field of slice dyeing, in particular to a slice dyeing device and a using method thereof.

Background

At the current state of the art, we will extract the right amount of abnormal cellular tissue and then make pathological sections by histopathological methods (typically we will soak these tissue samples in paraffin and then slice them with a slicing machine followed by staining with hematoxylin-eosin (H-E) dye to better observe the lesions). By analyzing the series of processes in detail, the existence and evolution process of the diseases can be determined, so that an accurate pathological diagnosis result is obtained. However, in this process, manually operated slice staining is often used in experiments, because the automatic staining machine is costly and complex to operate, and may not be suitable for common demonstration experiments or practical operations of students on a daily basis.

Chinese patent No.: CN202021186227.7 discloses a pathological section dyeing tank, which comprises a base, base top outer wall is provided with the staining jar, and two base top both sides outer walls all are provided with the slide rail, one side outer wall that the base is close to the slide rail is provided with rack board, two slide rails and rack board are relative one side and are provided with lift adjustment mechanism, and one side outer wall of lift adjustment mechanism is provided with the connecting plate, the bottom outer wall of connecting plate is provided with the carrier, two slide rail are relative one side outer wall and are provided with same fixed box, the positive outer wall of fixed box is provided with the hot plate, fixed box back outer wall has the chamber door through hinged joint, the staining jar top outer wall is opened there is the staining mouth, and the staining jar top outer wall is connected with the bull stick through the mounting, the equal swivelling joint in both ends outer wall of bull stick has same cylinder cap, cylinder cap one side outer wall bonds there is sealed pad. The utility model can effectively promote the rapid evaporation of the staining solution on the carrier, thereby ensuring the rapid transfer of the sections. However, when the method is used, the slices are required to be cleaned independently after being transferred, the process of taking out and cleaning the slices is troublesome, and the tissue of the slices is easily damaged due to improper operation, so that the whole experimental result is influenced.

Disclosure of Invention

The invention aims to overcome the difficulty in the background technology and provide a slice dyeing device and a use method thereof, wherein the slice dyeing operation is simple, and the cleaning process after dyeing is also simple.

In order to achieve the above purpose, the technical scheme adopted is as follows: the utility model provides a section dyeing apparatus, includes the box, the fixed dye vat that is equipped with in bottom half, the box is equipped with the dye box in dye vat top, the fixed elevating system that is equipped with in box and dye box outer wall connection make the vertical direction sliding connection of dye box in dye vat inner chamber, the dye box bottom is equipped with a plurality of feed liquor holes, the dye box bottom is equipped with the valve of switch feed liquor hole, dye box one side is equipped with articulated closing door, the joint face of closing door and dye box all is equipped with the sealing strip, dye box outer wall intercommunication has spraying mechanism, stoving mechanism and drain pipe.

Further, elevating system is two vertical electronic slide rails that box inner wall set up relatively, two all be equipped with the slider on the slide rail, two be connected with the connecting plate between the slider, the bottom of connecting plate is equipped with the top fixed connection of connecting rod and dyeing box, the slide rail connection has the driving piece, the driving piece is fixed the step motor of locating the box inner wall.

Further, the bottom of the dyeing box is fixedly provided with a cavity for placing a valve, the valve is a sliding piece which is connected to the inner wall of the cavity in a sliding way, and the sliding piece is provided with a plurality of liquid outlet holes which are arranged corresponding to the liquid inlet holes at the bottom of the dyeing box.

Further, the spraying mechanism comprises a liquid outlet pipe communicated with the top of the dyeing box, a spray head connected with the liquid outlet pipe is arranged on the inner wall of the top of the dyeing box, a sprayer is connected with the other end of the liquid outlet pipe, and liquid in the sprayer is set at normal temperature.

Further, the shower nozzle includes the feed liquor chamber with drain pipe intercommunication, feed liquor chamber bottom intercommunication has the stock solution chamber, the diameter in stock solution chamber is greater than the diameter in feed liquor chamber, the chamber bottom intercommunication in stock solution chamber has a plurality of branch liquid tanks, the intercommunication has the guide runner between branch liquid tank and the stock solution chamber, the guide runner is conical setting, the radius that the guide runner is close to the stock solution chamber end is less than the radius that is close to branch liquid tank end, the bottom of branch liquid tank is equipped with the nozzle, the radius of branch liquid tank is from last to reducing in proper order and setting down.

Further, the drying mechanism comprises an air outlet pipe communicated with the top of the dyeing box, the other end of the air outlet pipe is connected with a one-way blower, and the wind direction of the one-way blower is arranged in the dyeing box.

Further, the drain pipe is communicated with the bottom of the dyeing box.

Further, the front face of the box body is provided with a hinged glass door.

Further, the outer wall of the box body is provided with a controller, and the controller is connected with the blower, the sprayer and the driving piece.

Further, the outer wall of the sealing door is provided with a handle connected with the outer wall of the dyeing box.

The beneficial effects of adopting above-mentioned scheme are: when the slice dyeing device is used, slices which are dewaxed to water are placed in a glass slide, the glass slide is covered with a cover glass, the whole slice is placed in a slice box, a sealing door of the dyeing box is opened, the slice box is placed in the dyeing box, the sealing door is closed, a liquid inlet valve at the bottom of the dyeing box is opened, the dyeing box is lowered into a dyeing groove by opening a lifting mechanism, after dyeing is finished, the dyeing box is lifted to be separated from a dye solution of the dyeing groove by opening the lifting mechanism, the valve at the liquid inlet is closed, the whole dyeing box is in a sealing state, a spraying mechanism is opened, water vapor of the spraying mechanism is used for cleaning and decolorizing the glass slide dyed by the slice box in the dyeing box, water generated during the cleaning process is discharged out of the dyeing box from a drain pipe, a drying mechanism is opened after cleaning is finished, the water vapor attached to the glass slide is dried by the drying mechanism, and the sealing door is opened to take out the slice box after drying, so that the whole slice dyeing work is completed.

According to the method, the slice dyeing is carried out by putting the slice box into the dyeing box, the slide is cleaned by using water vapor after the slice dyeing, the water vapor molecular volume is smaller than the water molecular volume, the cleaning effect of the water vapor is better, the overall consumption of water is reduced when the water vapor is used for cleaning, water resources are saved, meanwhile, the cleaning link and the dyeing link are carried out in the dyeing box, the slice dyeing and cleaning are not required to be manually operated, the overall operation is simpler, the slice cleaning effect is better, the whole equipment is simple, and the method is suitable for laboratory and students with poor conditions to carry out slice dyeing experiments at ordinary times.

Drawings

FIG. 1 is a schematic view of a slice staining apparatus and a method of using the same.

Fig. 2 is a schematic diagram of the internal structure of fig. 1.

FIG. 3 is a schematic view of the bottom of the staining cassette and the slide.

Fig. 4 is a schematic view of the bottom of the staining cassette after connection to the slide.

Fig. 5 is a schematic structural diagram of a spray head in the third embodiment.

In the figure, 1, a blower; 2. a case; 3. a controller; 4. a liquid outlet pipe; 5. an air outlet pipe; 6. a staining cassette; 7. a cavity; 8. a drain pipe; 9. a dyeing tank; 10. a slide rail; 11. a sliding block; 12. a connecting plate; 13. a spraying machine; 14. closing the door; 15. a sealing strip; 16. a liquid inlet hole; 17. a liquid outlet hole; 18. a slide sheet; 19. a spray head; 20. a liquid storage cavity; 21. a flow guide channel; 22. a liquid dividing tank; 23. a nozzle; 24. a liquid inlet cavity.

Detailed Description

The technical scheme of the present invention will be clearly and completely described in the following in connection with the specific embodiments of the present invention. The described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Example 1

1-2, A section dyeing device, including box 2, characterized by:

the fixed dye vat 9 that is equipped with in box 2 bottom, box 2 is equipped with dye box 6 in dye vat 9 top, box 2 internal fixation is equipped with elevating system and dye box 6 external wall connection, make dye box 6 vertical direction sliding connection in dye vat 9 inner chamber, elevating system is two electronic slide rails 10 of the vertical setting in box 2 inner wall left and right sides, all be equipped with the slider on two slide rails 10, be connected with connecting plate 12 between two sliders, the bottom of connecting plate 12 is equipped with the top fixed connection of connecting rod and dye box 6, slide rail 10 is connected with the driving piece, the driving piece is fixed the step motor of locating box 2 inner wall.

As shown in fig. 3-4, a plurality of liquid inlets 16 are formed in the bottom of the dyeing box 6, a cavity 7 for placing a valve is fixedly formed in the bottom of the dyeing box 6, a sliding piece 18 which is connected to the inner wall of the cavity 7 in a sliding mode is arranged in the cavity 7, and a plurality of liquid outlets 17 which are arranged corresponding to the liquid inlets 16 in the bottom of the dyeing box 6 are formed in the sliding piece 18.

One side of the dyeing box 6 is provided with a hinged sealing door 14, the connecting surface of the door and the dyeing box 6 is provided with sealing strips 15, the sealing strips 15 can enable the sealing effect of the connecting surface of the sealing door 14 and the dyeing box 6 to be better, and the outer wall of the sealing door 14 is provided with a handle connected with the outer wall of the dyeing box 6.

As shown in fig. 1-2, the outer wall of the dyeing box 6 is communicated with a spraying mechanism 13, a drying mechanism and a drain pipe 8, the spraying mechanism 13 comprises a liquid outlet pipe 4 communicated with the top of the dyeing box 6, the inner wall of the top of the dyeing box 6 is provided with a spray head 19 connected with the liquid outlet pipe 4, the other end of the liquid outlet pipe 4 is connected with a spraying mechanism 13, and liquid in the spraying mechanism 13 is set at normal temperature. The drying mechanism comprises an air outlet pipe 5 communicated with the top of the dyeing box 6, the other end of the air outlet pipe 5 is connected with a unidirectional blower 1, and the wind direction of the unidirectional blower 1 is arranged in the dyeing box 6. The drain pipe 8 is communicated with the bottom of the dyeing box 6.

In practice, the sprayer 13, the electric slide rail 10 and the unidirectional blower 1 are all of the prior art and can be purchased directly from the market, and are not described herein.

In specific implementation, the sealing strip 15 is made of rubber.

The specific implementation mode is as follows: placing slices dewaxed to water on a glass slide, covering the glass slide, integrally placing the slices into a slice box (the slice box is a box for clamping broken pieces for dyeing commonly used in the market), opening a sealing door 14 of a dyeing box 6, placing the slice box into the dyeing box 6, closing the sealing door 14, arranging a sliding piece 18 in a cavity 7 at the bottom of the dyeing box 6 to enable a liquid inlet to overlap with a liquid outlet, enabling the bottom of the dyeing box 6 to keep the dye in a dyeing groove 9 to enter the dyeing box 6, starting a stepping motor to enable a sliding block to slide downwards from a sliding rail 10, and enabling the sliding block to drive the dyeing box 6 to descend and dip into the dyeing groove 9;

After dyeing is finished, the stepping motor is started to enable the sliding rail 10 to drive the sliding block to slide upwards, the dyeing box 6 is enabled to ascend and separate from the dyeing liquid of the dyeing groove 9, meanwhile, the sliding block 11 in the cavity 7 of the dyeing box 6 is slid to be staggered with the liquid inlet and the liquid outlet, the effect of sealing the dyeing box 6 is achieved, the whole dyeing box 6 is in a sealed state, the sprayer 13 is started, water vapor of the sprayer 13 washes and decolors a slide dyed by the slice box in the dyeing box 6, sewage generated during the process is discharged from the drain pipe 8 to the dyeing box 6, the dryer is started after washing is finished, the drying mechanism dries the water vapor attached to the slide, the sealing door is opened after drying, the slice box is taken out, and the whole slice dyeing work is completed.

According to the method, the slice dyeing is achieved by only putting the slice box into the dyeing box 6, water vapor is used for cleaning the glass slide after the slice dyeing, the water vapor molecular volume is smaller than the water molecular volume, the water vapor infiltration effect is better, the overall cleaning effect is better, the overall consumption of water is also reduced when the water vapor is used for cleaning, water resources are saved, meanwhile, the cleaning link and the dyeing link are carried out in the dyeing box 6, the slice dyeing and cleaning do not need to be manually operated, the overall operation is simpler, the slice cleaning effect is better, the whole equipment is simple to prepare, and the method is suitable for the slice dyeing experiment in laboratories and students with poor most conditions at ordinary times.

Example two

On the basis of the first embodiment, as shown in fig. 1, the applicant provides a controller 3 on the outer wall of the case 2, and the controller 3 connects the blower 1, the sprayer 13 and the driving member. After application, the whole cleaning and dyeing process can be directly controlled by the slave operation controller 3, the operation is simpler, and more people are applicable.

In the specific implementation, the inner cavity at the bottom of the dyeing box 6 can be provided with a motor driving mechanism to slidingly connect the sliding piece 18 with the inner cavity, and after the setting, the sliding piece 18 does not need to be manually pushed and pulled by hands, so that the operation process is simpler. This arrangement is not described herein as it could be implemented in the prior art.

When the concrete implementation, the front of box 2 is equipped with articulated glass door, and the setting of glass door can let whole dyeing experiment keep sealed, improves the security of experiment, and the glass door can see the inside condition of whole experimental process simultaneously, conveniently carries out the timely operation processing of experimental process, and the practicality is stronger.

Example III

On the basis of the first embodiment and the second embodiment, the applicant finds that, after a plurality of dyeing experiments, because the sealing environment of the dyeing box 6 is small, water vapor sprayed from the liquid outlet pipe 4 in the dyeing box 6 is easily condensed into water drops when sprayed, so that the spraying range of the whole water vapor is reduced, and further the cleaning effect of the slice is not ideal or the cleaning time is prolonged, thereby affecting the whole experiment.

In view of the above problems, the applicant found that the applicant made an improvement in the problem because the water vapor discharged from the atomizer 13 had a too high flow rate, resulting in a too high water vapor discharge rate and condensation with each other.

Referring to fig. 5, the spray head 19 includes a liquid inlet cavity 24 communicated with the liquid outlet pipe 4, a liquid storage cavity 20 is communicated with the bottom of the liquid inlet cavity 24, a plurality of liquid separating tanks 22 are communicated with the bottom of the liquid storage cavity 20, a flow guiding channel 21 is communicated between the liquid separating tanks 22 and the liquid storage cavity 20, the flow guiding channel 21 is in a conical shape, the radius of the flow guiding channel 21 near the end of the liquid storage cavity 20 is smaller than the radius near the end of the liquid separating tank 22, a nozzle 23 is arranged at the bottom of the liquid separating tank 22, and the radius of the liquid separating tank 22 is sequentially reduced from top to bottom.

In particular, a water pump is provided in the sprayer 13.

According to the above arrangement, after the sprayer 13 is started, water in the liquid outlet pipe 4 enters the liquid inlet cavity 24, water in the liquid inlet cavity 24 enters the liquid storage cavity 20, the diameter of the liquid inlet cavity 24 is smaller than that of the liquid storage cavity 20, the water flow in the liquid inlet cavity 24 can slow down at a certain speed, the water flows into the liquid separation grooves 22 through the flow guide channels 21, the radius of the flow guide channels 21 near the end of the liquid storage cavity 20 is smaller than that near the end of the liquid separation grooves 22, the water flow is changed from small to large, the flow speed can be changed from small to slow down, and meanwhile, when the water flows from the liquid separation grooves 22 to the nozzles 23 at the bottom of the liquid separation grooves 22, the flow speed of the water can be reduced from large to small, the water flow speed in the spray heads 19 can be slowed down as a whole, so that the water vapor speed sprayed out of the spray heads 23 is controlled, the water vapor between the spray nozzles 23 is not easy to be condensed, the spreading condition of the sprayed water mist is ensured, the continuous water mist cleaning effect in the dyeing box 6 is ensured, and the slice cleaning capability is improved.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims (10)

1. The utility model provides a section dyeing apparatus, includes box (2), characterized by:

The utility model discloses a dyeing machine, including box (2), box (6), sealing strip (15) are all equipped with in the connection face of box (6) in box (2) top, box (2) internal fixation is equipped with elevating system and dyeing box (6) outer wall connection, makes dyeing box (6) vertical direction sliding connection in dyeing groove (9) inner chamber, dyeing box (6) bottom is equipped with a plurality of feed liquor holes (16), dyeing box (6) bottom is equipped with the valve of switch feed liquor hole (16), dyeing box (6) one side is equipped with articulated closing door (14), closing door (14) all are equipped with sealing strip (15) with dyeing box (6) the connection face, dyeing box (6) outer wall intercommunication has sprayer (13) to construct, stoving mechanism and drain pipe (8).

2. The apparatus for staining sections according to claim 1, wherein: the lifting mechanism is characterized in that the lifting mechanism is provided with two vertical electric sliding rails (10) which are oppositely arranged on the inner wall of the box body (2), the sliding rails (10) are respectively provided with a sliding block, a connecting plate (12) is connected between the sliding blocks, the bottom of the connecting plate (12) is provided with a connecting rod and fixedly connected with the top of the dyeing box (6), the sliding rails (10) are connected with driving pieces, and the driving pieces are stepping motors fixedly arranged on the inner wall of the box body (2).

3. The apparatus for staining sections according to claim 1, wherein: the dyeing box is characterized in that a cavity (7) for placing a valve is fixedly arranged at the bottom of the dyeing box (6), the valve is a sliding sheet (18) which is connected to the inner wall of the cavity (7) in a sliding mode, and a plurality of liquid outlet holes (17) which are arranged corresponding to liquid inlet holes (16) at the bottom of the dyeing box (6) are formed in the sliding sheet (18).

4. The apparatus for staining sections according to claim 1, wherein: the sprayer (13) comprises a liquid outlet pipe (4) communicated with the top of the dyeing box (6), a spray head (19) connected with the liquid outlet pipe (4) is arranged on the inner wall of the top of the dyeing box (6), the other end of the liquid outlet pipe (4) is connected with the sprayer (13), and liquid in the sprayer (13) is set at normal temperature.

5. The apparatus for staining sections according to claim 4, wherein: the shower nozzle (19) include feed liquor chamber (24) with drain pipe (4) intercommunication, feed liquor chamber (24) bottom intercommunication has stock solution chamber (20), the diameter of stock solution chamber (20) is greater than the diameter of feed liquor chamber (24), the chamber bottom intercommunication of stock solution chamber (20) has a plurality of branch cistern (22), the intercommunication has guide runner (21) between branch cistern (22) and stock solution chamber (20), guide runner (21) are conical setting, the radius that guide runner (21) are close to stock solution chamber (20) end is less than the radius that is close to branch cistern (22) end, the bottom of branch cistern (22) is equipped with nozzle (23), the radius of branch cistern (22) is from last to reduce in proper order and set up down.

6. The apparatus for staining sections according to claim 1, wherein: the drying mechanism comprises an air outlet pipe (5) communicated with the top of the dyeing box (6), the other end of the air outlet pipe (5) is connected with a unidirectional air blower (1), and the wind direction of the unidirectional air blower (1) is arranged in the dyeing box (6).

7. The apparatus for staining sections according to claim 1, wherein: the drain pipe (8) is communicated with the bottom of the dyeing box (6).

8. The apparatus for staining sections according to claim 1, wherein: the front face of the box body (2) is provided with a hinged glass door.

9. The slice staining apparatus of claim 2, 5 or 6, wherein: the outer wall of the box body (2) is provided with a controller (3), and the controller (3) is connected with the air blower (1), the sprayer (13) and the driving piece.

10. The apparatus for staining sections according to claim 1, wherein: the outer wall of the sealing door (14) is provided with a handle connected with the outer wall of the dyeing box (6).