CN118213025A - Nucleic acid detection information management method - Google Patents
Nucleic acid detection information management method Download PDFInfo
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- CN118213025A CN118213025A CN202410156233.4A CN202410156233A CN118213025A CN 118213025 A CN118213025 A CN 118213025A CN 202410156233 A CN202410156233 A CN 202410156233A CN 118213025 A CN118213025 A CN 118213025A
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- 238000001514 detection method Methods 0.000 title claims abstract description 168
- 238000007726 management method Methods 0.000 title claims abstract description 60
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 59
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 59
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 claims abstract description 35
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 238000004458 analytical method Methods 0.000 claims abstract description 25
- 238000005070 sampling Methods 0.000 claims abstract description 13
- 230000002159 abnormal effect Effects 0.000 claims description 20
- 238000012216 screening Methods 0.000 claims description 9
- 238000012795 verification Methods 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 5
- 238000012544 monitoring process Methods 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 230000002950 deficient Effects 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 description 8
- 238000007405 data analysis Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
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Abstract
The invention relates to the technical field of medical detection, in particular to a nucleic acid detection information management method. The invention specifically comprises a sampling management stage, a detection management stage, an analysis management stage and a detection report generation stage; the method comprises the steps of scanning a bar code of a nucleic acid detection test tube in a sampling management stage and inputting test tube information, typesetting the test tube information in a detection management stage, extracting nucleic acid, detecting on-machine by using a PCR instrument, inputting detection information, analyzing off-machine data of the detection information in an analysis management stage, deriving CT values according to the off-machine data obtained by analysis, and generating a health supervision report according to the generated CT values in a detection report generation stage. The invention provides a set of standardized nucleic acid detection platform for automatically processing nucleic acid detection information by carrying out the collection and management of the information of the whole flow, so as to further improve the efficiency of nucleic acid collection and the quality of the collected information.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to a nucleic acid detection information management method.
Background
At present, the nucleic acid detection is usually carried out by inputting and collecting artificial information, and the problems of lower efficiency, lack of accuracy and safety of information input and easiness in occurrence of artificial errors exist when the multi-person nucleic acid detection is carried out. Meanwhile, in the process of information management and unified information collection of the artificial nucleic acid detection in the later stage, a set of standardized operation system is also lacking for unified nucleic acid detection information screening and maintenance management operation, so that the problem that the release of nucleic acid detection data results is slow, the screening operation of a retest sample needs to be manually carried out, a large amount of manpower and material resource cost is consumed, the targeted management of sample information cannot be further realized, the efficient push management of result information cannot be further improved, and a standardized and standardized management system cannot be formed is solved.
The Chinese patent with publication number CN113506087A provides a nucleic acid detection acquisition system and a control method thereof, wherein the acquisition, storage and access of the nucleic acid detection data and other modules are established in the patent so as to further improve the input efficiency and input accuracy of the nucleic acid. However, in the patent, only the data input process is managed, the input accuracy in the data input process is not monitored, and the accurate identification and management of abnormal data in the data input process cannot be further ensured. The Chinese patent with publication No. CN113444625A provides an automatic nucleic acid detection system and method, in which the nucleic acid extraction stage is used for implementing automatic nucleic acid analysis and detection by establishing an automatic control subsystem for analysis and detection treatment of nucleic acid samples, but the automatic control subsystem described in the patent is not related to the problems of detection times and rechecking, so that it is impossible to determine how to control the accuracy of detection in the process of nucleic acid analysis and detection.
Accordingly, in view of the problems with existing nucleic acid detection management systems, the present invention provides a method for managing nucleic acid detection information.
Disclosure of Invention
Aiming at the problems, the invention provides a nucleic acid detection information management method, which specifically comprises a sampling management stage, a detection management stage, an analysis management stage and a detection report generation stage; the method comprises the steps of scanning a bar code of a nucleic acid detection test tube in a sampling management stage and inputting test tube information, typesetting the test tube information in a detection management stage, extracting nucleic acid, detecting on-machine by using a PCR instrument, inputting detection information, analyzing off-machine data of the detection information in an analysis management stage, deriving CT values according to the off-machine data obtained by analysis, and generating a health supervision report according to the generated CT values in a detection report generation stage.
Preferably, the specific flow of the sampling management stage is as follows:
s1.1, performing initial sample collection, scanning a test tube bar code;
s1.2, scanning test tube bar codes, and carrying out automatic plate arrangement operation in a manner of being compatible with various code scanning tools;
s1.3, carrying out sample collection and rechecking, and checking whether a bar code exists or not during the sample collection and rechecking;
S1.4, automatic typesetting, including plate type switching and positioning typesetting;
s1.5, when the unqualified sample is identified, automatic registration of the unqualified sample is performed.
Preferably, the detection management stage comprises test tube hole site positioning and abnormal test tube hole site reinspection; and the hole site information of the test tube is automatically summarized.
Preferably, the specific flow of the detection management stage is as follows:
s2.1, performing detection primary detection, accurately identifying and positioning hole site information, extracting nucleic acid from a sample, and detecting the sample;
s2.2, setting detection rechecking operation for positive samples, unqualified samples and abnormal hole site samples in the nucleic acid extraction result in the detection initial detection process;
S2.3, the detection and rechecking result inquiry is carried out according to the sample receiving time, the detection result, the order state and the detection area, and the rechecking result inquiry comprises a rechecking plate number and a hole site number, a primary checking plate number and a hole site number, and the rechecking result and the primary checking result.
Preferably, the PCR instrument data are read to judge the negative and positive detection results according to the judgment rule of the kit, and the recheck is set according to the positive sample and the unqualified sample in the detection result.
Preferably, the specific procedure of rechecking the positive samples and the unqualified samples comprises the following steps:
s3.1, firstly, importing PCR instrument data into a system, screening and prompting abnormal hole site numbers and abnormal reasons, wherein the abnormal reasons comprise positive or unqualified samples;
s3.2, checking abnormal positive and sample unqualified data obtained through screening, and setting rechecking by one key;
S3.3, carrying out multiple verification on the sample to be re-inspected.
Preferably, the detection information is subjected to the next machine data analysis in the analysis management stage, and the CT value is derived according to the next machine data obtained by the analysis.
Preferably, in the detection report generation step, a determination frame is generated from a determination method in the detection reagent specification, and a detection result is automatically generated from the generated CT value using the determination frame.
Preferably, in the detection report generation stage, after the automatically generated detection result is generated into the health supervision report, the health supervision report is pushed to the local area health supervision system.
Preferably, the specific flow of the detection report generation stage is as follows:
S4.1 pushing information into a local area health supervision system, wherein the pushing information comprises a current-day detection result and a reinspection detection result, and a pushing data reconfirmation function is established;
The pushing information in S4.2 is used for further checking the nucleic acid data by pushing the negative sample and the positive sample respectively, so that the problem of pushing errors is avoided;
s4.3, a data board is established for displaying the detection condition of the nucleic acid in real time, the data board is established for monitoring the progress of sample collection and typesetting and detecting the analysis progress, and the unreceived sample box codes are prompted on the data board.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention provides a nucleic acid detection information management method, which is used for carrying out the whole-flow information acquisition and management work of an analysis management stage and a detection report generation stage in a sampling management stage, a detection management stage and a detection report generation stage involved in the nucleic acid detection process, so as to provide a standardized nucleic acid detection platform for carrying out the automatic processing of the nucleic acid detection information and further improving the efficiency of nucleic acid acquisition and the quality of the acquired information.
(2) On the basis of the step (1), the typesetting can be flexibly adjusted according to actual requirements in the sampling management stage, verification is carried out according to different bar code rules during code scanning input, input errors are avoided, the initial sample collection detection and the repeated sample collection detection are simultaneously set, and a kit different from the initial sample detection is selected during the repeated sample collection detection, so that the accuracy of sample collection data is further improved.
(3) On the basis of the step (2), the invention performs initial detection and repeated detection in the detection management stage, and performs multiple verification on unqualified samples in the repeated detection, thereby facilitating sample picking and preventing the occurrence of detection errors.
(4) In the detection report generation stage, detection results are automatically generated through a judgment framework and pushed to a local area health supervision system, the nucleic acid detection condition is displayed in real time through a data signboard, the progress of the housing typesetting and the detection progress analysis are monitored, and the timeliness and the effectiveness of data are ensured.
Drawings
FIG. 1 is a flowchart of a method for managing nucleic acid detection information.
Detailed Description
In this embodiment, a method for managing nucleic acid detection information is provided, as shown in fig. 1, and specifically includes a sampling management stage, a detection management stage, an analysis management stage, and a detection report generation stage.
< Sampling management phase >
Scanning a nucleic acid detection test tube bar code and inputting test tube information in a sample collection management stage, and performing sample collection initial detection and sample collection repeated detection aiming at the problem that manual errors easily exist in the input and sample collection of the manual information of the nucleic acid detection in the sample collection management stage, so that the occurrence of the manual errors in the input and sample collection processes is reduced.
The concrete flow of the sampling management stage is as follows:
S1.1, performing initial sample collection, scanning a test tube bar code, typesetting an original sample received by the scanning bar code into an 8 x 12 test tube rack, and adding the sample into an 8 x 12 experimental plate;
S1.2, in the scanning test tube bar code, a plurality of code scanning tools are compatible, automatic plate arrangement operation is carried out, and the sample adding speed is further improved, wherein bar code rules comprise, but are not limited to, health cloud and east soft bar code rules, different verification modes are set for different bar code rules, and abnormal bar codes are automatically jumped to manual input verification;
s1.3, carrying out sample collection and rechecking, wherein whether a bar code exists or not is checked during the sample collection and rechecking, and when a kit is not designated by default, a kit which is different from that during the initial sample collection and rechecking is required to be selected during the sample collection and rechecking;
s1.4, automatic typesetting comprises plate type switching and positioning typesetting, wherein the plate type switching carries out the custom switching of the experimental plates with 96/100 holes, in the positioning typesetting, the initial position is designated to start typesetting, and meanwhile, the position is designated to be withdrawn;
S1.5, when an unqualified sample is identified, automatically registering the unqualified sample, wherein unqualified reasons of the unqualified sample comprise sample damage, sample leakage, no internal standard is obtained after rechecking, a test tube has no swab, the test tube has sample registration information, a sending person does not send the sample, and other reasons are invalidated.
< Detection management phase >
In the detection management stage, typesetting test tube information, in the typesetting of the test tube information, the custom switching of the 96/100-hole experimental plate is carried out, and meanwhile, the test tube is subjected to positioning selection to be used for designating the initial position and the withdrawal position for carrying out custom typesetting operation. Performing nucleic acid extraction and on-machine detection by using a PCR instrument, and inputting detection information, wherein the detection management stage comprises test tube hole site positioning and abnormal test tube hole site detection re-detection; and the hole site information of the test tube is automatically summarized.
The specific process of the detection management stage is as follows:
s2.1, performing detection primary detection, accurately identifying and positioning hole site information, extracting nucleic acid from a sample, and detecting the sample;
S2.2, setting detection rechecking operation on positive samples, unqualified samples and abnormal hole site samples in the nucleic acid extraction result;
S2.3, the detection and rechecking result inquiry is carried out according to the sample receiving time, the detection result, the order state and the detection area, the detection and rechecking result inquiry comprises a rechecking plate number and a hole site number, a primary detection plate number and a hole site number, and the detection and rechecking result and the primary detection result.
Specifically, the method comprises the steps of reading PCR instrument data, judging a negative and positive detection result according to a kit judgment rule, and setting detection rechecking according to a positive sample and a disqualified sample in the detection result, wherein the specific process of rechecking the positive sample and the disqualified sample comprises the following steps:
s3.1, firstly, importing PCR instrument data into a system, screening and prompting abnormal hole site numbers and abnormal reasons, wherein the abnormal reasons comprise positive or unqualified samples;
s3.2, checking abnormal positive and sample unqualified data obtained through screening, and setting rechecking by one key;
s3.3, carrying out multiple verification on the detection reinspection sample, wherein the multiple verification process comprises the following steps:
s3.3.1, providing a sample detection initial inspection typesetting position, facilitating the picking and inspection and avoiding error;
s3.3.2, if the primary inspection typesetting is found to have dislocation when the re-inspection samples are picked, setting the common re-inspection operation of surrounding area samples, and screening potential error samples;
s3.3.3, setting the hole site where the repeated inspection typesetting and code scanning are completed as typesetting states, and simultaneously refreshing the pages to remove typesetting pages until the typesetting pages are emptied, so that omission of the repeated inspection data to be inspected is avoided.
The typesetting page comprises a primary detection plate number, a primary detection 8 x 12 hole site number, a bar code number, a primary detection 10 x 10 hole site number, a final result, an order state and a region. Meanwhile, through targeted setting of a query interval, a review result query is conducted, and in the review result query, corresponding results are searched for according to the sample receiving time, the detection result, the order state and the detection area.
< Analysis management phase >
And in the analysis management stage, performing off-machine data analysis on the detection information, and deriving CT values according to the off-machine data obtained by the analysis.
< Detection report generation stage >
And generating a health supervision report according to the generated CT value in the detection report generation stage. In the detection report generation stage, a determination frame is generated by using a determination mode in the detection reagent specification, and a detection result is automatically generated by using the determination frame according to the generated CT value. The detection report generation stage is used for pushing the automatically generated detection result to a local area health supervision system after generating a health supervision report; wherein the local area health supervision system comprises a government specified full-field Jing Yiqing pathogen detection information system.
The specific flow of the detection report generation stage is as follows:
S4.1, pushing the comparison result to a local area health supervision system, wherein the pushing information comprises a current-day detection result and a reinspection detection result, and a pushing data reconfirmation function is established;
s4.2, pushing the negative sample and the positive sample respectively to further check the nucleic acid data, so that the problem of wrong pushing is avoided;
S4.3, the method is used for displaying the nucleic acid detection condition in real time by establishing the data board, monitoring the sample collection typesetting progress and the analysis progress by establishing the data board, and prompting the unreceived sample box codes on the data board.
The data board is used for counting nucleic acid results, wherein the nucleic acid result statistics comprises negative quantity, primary detection positive quantity, secondary detection positive quantity, primary detection unqualified quantity and secondary detection unqualified quantity.
On the basis, the latest sample receiving time, the latest typesetting time and the latest importing time are set up to update the data of the data signboard in a specified period, and the detection progress of the same day is monitored in real time, wherein the specified period is automatically updated at intervals of 5 minutes.
Meanwhile, the input data is classified and counted according to the order sources, and the red fields are clicked to display classification and counting details.
In one embodiment, the order sources include, but are not limited to, east soft total, healthy cloud total, physical examination total. The classification statistics include, but are not limited to, non-typeset, entered, pushed, failed push, failed sample, matched, unmatched, and total number of bars.
The method comprises the steps that the number of the un-pushed hours is displayed specifically, so that detection overtime is avoided by inquiring the number of the un-pushed hours; the unmatched quantity of the hours is specifically displayed to inquire the unmatched quantity, so that detection overtime is avoided; the total number is specifically displayed for counting the number of the receivable and unreceived samples according to the region, and the unreceived bin codes are specifically displayed for inquiring the typeset bin codes and the number of the unreceived bin codes and the samples, reminding timely receiving and avoiding overtime of inspection.
Claims (10)
1. The nucleic acid detection information management method is characterized by comprising a sampling management stage, a detection management stage, an analysis management stage and a detection report generation stage; the method comprises the steps of scanning a bar code of a nucleic acid detection test tube in a sampling management stage and inputting test tube information, typesetting the test tube information in a detection management stage, extracting nucleic acid, detecting on-machine by using a PCR instrument, inputting detection information, analyzing off-machine data of the detection information in an analysis management stage, deriving CT values according to the off-machine data obtained by analysis, and generating a health supervision report according to the generated CT values in a detection report generation stage.
2. The method for managing nucleic acid detecting information according to claim 1, wherein the specific flow of the sampling management stage is as follows:
s1.1, performing initial sample collection, scanning a test tube bar code;
s1.2, scanning test tube bar codes, and carrying out automatic plate arrangement operation in a manner of being compatible with various code scanning tools;
s1.3, carrying out sample collection and rechecking, and checking whether a bar code exists or not during the sample collection and rechecking;
S1.4, automatic typesetting, including plate type switching and positioning typesetting;
s1.5, when the unqualified sample is identified, automatic registration of the unqualified sample is performed.
3. The method according to claim 1, wherein the detection management stage comprises positioning of test tube hole sites and reinspection of abnormal test tube hole sites; and the hole site information of the test tube is automatically summarized.
4. The method for managing nucleic acid detection information according to claim 3, wherein the specific flow of the detection management stage is as follows:
s2.1, performing detection primary detection, accurately identifying and positioning hole site information, extracting nucleic acid from a sample, and detecting the sample;
s2.2, setting detection rechecking operation for positive samples, unqualified samples and abnormal hole site samples in the nucleic acid extraction result in the detection initial detection process;
S2.3, the detection and rechecking result inquiry is carried out according to the sample receiving time, the detection result, the order state and the detection area, and the rechecking result inquiry comprises a rechecking plate number and a hole site number, a primary checking plate number and a hole site number, and the rechecking result and the primary checking result.
5. The method according to claim 4, wherein the PCR instrument data is read to determine the negative and positive detection results according to the kit determination rule, and the recheck is set according to the positive sample and the defective sample.
6. The method according to claim 5, wherein the specific procedure for rechecking the positive samples and the unqualified samples comprises:
s3.1, firstly, importing PCR instrument data into a system, screening and prompting abnormal hole site numbers and abnormal reasons, wherein the abnormal reasons comprise positive or unqualified samples;
s3.2, checking abnormal positive and sample unqualified data obtained through screening, and setting rechecking by one key;
S3.3, carrying out multiple verification on the sample to be re-inspected.
7. The method according to claim 1, wherein the analysis management stage analyzes the detection information with the next machine data, and derives the CT value based on the next machine data obtained by the analysis.
8. The method for managing nucleic acid detection information according to claim 7, wherein the detection report generation step generates a determination frame from a determination pattern in a detection reagent specification, and automatically generates a detection result based on the generated CT value using the determination frame.
9. The method according to claim 8, wherein the detection report generation step is performed by pushing the automatically generated detection result to the local area health monitoring system after generating the health monitoring report.
10. The method for managing nucleic acid detection information according to claim 9, wherein the specific flow of the detection report generation phase is as follows:
S4.1 pushing information into a local area health supervision system, wherein the pushing information comprises a current-day detection result and a reinspection detection result, and a pushing data reconfirmation function is established;
The pushing information in S4.2 is used for further checking the nucleic acid data by pushing the negative sample and the positive sample respectively, so that the problem of pushing errors is avoided;
s4.3, a data board is established for displaying the detection condition of the nucleic acid in real time, the data board is established for monitoring the progress of sample collection and typesetting and detecting the analysis progress, and the unreceived sample box codes are prompted on the data board.
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