CN118161666A - Preparation method of platelet-rich plasma gel compound for facial filling - Google Patents
Preparation method of platelet-rich plasma gel compound for facial filling Download PDFInfo
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- CN118161666A CN118161666A CN202410204122.6A CN202410204122A CN118161666A CN 118161666 A CN118161666 A CN 118161666A CN 202410204122 A CN202410204122 A CN 202410204122A CN 118161666 A CN118161666 A CN 118161666A
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- 210000004623 platelet-rich plasma Anatomy 0.000 title claims abstract description 48
- 108010088880 plasmagel Proteins 0.000 title claims abstract description 33
- 238000011049 filling Methods 0.000 title claims abstract description 28
- 230000001815 facial effect Effects 0.000 title claims abstract description 27
- 150000001875 compounds Chemical class 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 5
- 102000009123 Fibrin Human genes 0.000 claims abstract description 25
- 108010073385 Fibrin Proteins 0.000 claims abstract description 25
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 claims abstract description 25
- 210000004369 blood Anatomy 0.000 claims abstract description 25
- 239000008280 blood Substances 0.000 claims abstract description 25
- 229950003499 fibrin Drugs 0.000 claims abstract description 25
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 23
- 210000002381 plasma Anatomy 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 17
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 15
- 238000000926 separation method Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 23
- 239000000203 mixture Substances 0.000 claims description 10
- 239000003146 anticoagulant agent Substances 0.000 claims description 3
- 229940127219 anticoagulant drug Drugs 0.000 claims description 3
- 230000003796 beauty Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003102 growth factor Substances 0.000 description 10
- 210000003205 muscle Anatomy 0.000 description 6
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 210000003491 skin Anatomy 0.000 description 5
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000703 high-speed centrifugation Methods 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
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- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
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- 238000010255 intramuscular injection Methods 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a preparation method of a platelet-rich plasma gel compound for facial filling, which comprises the following steps: placing the collected blood in a container under aseptic conditions; placing the container containing blood in a centrifuge for centrifugal separation; after centrifugal separation, the blood in the container is divided into three layers, namely a plasma layer, a platelet layer, a leucocyte layer, a fibrin layer and a erythrocyte layer from top to bottom; extracting the plasma layer, blood platelet, white cell and fibrin layer with a syringe respectively, heating the syringe containing the plasma layer in water bath, and cooling the plasma layer to form gel; mixing the gel with blood platelet, leucocyte and fibrin layer via medical three-way pipe to obtain the blood platelet-rich plasma gel compound. The platelet-rich plasma gel compound prepared by the invention is rich in platelets, white blood cells and fibrin, can be injected into various layers of skin, and plays a role in filling.
Description
Technical Field
The invention relates to the technical field of biomedical materials, in particular to a preparation method of a platelet-rich plasma gel compound for facial filling.
Background
Platelet-rich plasma (PRP) is a platelet concentrate obtained by extracting autologous venous blood and adopting a density gradient centrifugation method, is rich in various bioactive substances such as growth factors, cytokines, antibacterial peptides and the like, and has the effects of promoting cell proliferation, differentiation, matrix synthesis, tissue regeneration, repair and the like.
Currently, platelet rich plasma is mostly extracted by high-speed centrifugation. Such extraction methods are prone to platelet destruction. Furthermore, platelet rich plasma extracted according to such methods has the following drawbacks: (1) is almost free of white blood cells and fibrin; (2) The growth factors in the platelet-rich plasma are released quickly, the release time is 12-24 hours, and the slow release effect is relatively poor; (3) Only liquid smearing or injection can be carried out, and the injection layer is only a dermis layer, so that the filling effect cannot be achieved.
And secondly, the white blood cells can remove harmful substances and necrotic tissues, resist invasion of pathogenic agents and participate in repair and regeneration of the tissues. Fibrin can form a scaffold that loads platelet rich plasma, provides facial filling support and allows slow release of growth factors within the platelet rich plasma. The platelet-rich plasma prepared by the high-speed centrifugal separation method lacks white blood cells and fibrin, and cannot obtain the beneficial effects of the white blood cells and the fibrin.
Therefore, there is an urgent need to provide a method for preparing a platelet-rich plasma gel complex useful for facial filling, having a slow release of growth factors in platelet-rich plasma.
Disclosure of Invention
The present invention has been made in view of the above problems, and has as its object to provide a method for preparing a platelet rich plasma gel-complex for facial filling which overcomes or at least partially solves the above problems.
In a first aspect, embodiments of the present invention provide a method for preparing a platelet rich plasma gel-composite for facial filling, comprising the steps of:
step 1), placing collected blood in a container under a sterile condition;
step 2), placing the container containing blood in a centrifugal machine for centrifugal separation;
step 3), after centrifugal separation, the blood in the container is divided into three layers, namely a plasma layer, a platelet layer, a leucocyte layer, a fibrin layer and a erythrocyte layer from top to bottom;
step 4), respectively extracting the plasma layer, the platelet, the leucocyte and the fibrin layer by using a syringe, heating the syringe containing the plasma layer in water bath, and cooling the plasma layer to form gel;
Step 5), mixing the gel with the platelet, the white blood cell and the fibrin layer through a medical three-way tube to obtain the platelet-rich plasma gel compound.
Preferably, the container is a centrifuge tube or a blood collection tube without anticoagulant.
Preferably, the blood is autologous venous blood of the person seeking beauty.
Preferably, the centrifugal force of the centrifugal machine in the step 2) is 150 Xg-300 Xg, and the centrifugal time is 4-11 minutes.
Preferably, the centrifugal force of the centrifuge in step 2) is 200 Xg and the centrifugation time is 8 minutes.
Preferably, the temperature of the water bath heating in the step 4) is 60-80 ℃; the heating time is 8-10 minutes.
Preferably, the temperature of the water bath heating in the step 4) is 70 ℃; the heating time was 9 minutes.
Preferably, in the step 5), the gel, the platelets, the white blood cells and the fibrin layer are mixed by a medical three-way tube in a volume ratio of 4:1.
In a second aspect, the embodiment of the invention also provides a use of the platelet-rich plasma gel compound prepared by the method for preparing the platelet-rich plasma gel compound for facial filling according to any one of the above aspects in medical cosmetology.
The platelet-rich plasma gel compound prepared by the method provided by the embodiment of the invention is rich in platelets, white blood cells and fibrin, can be injected into various layers of skin, and plays a role in filling; secondly, the release time of the growth factors in the platelet-rich plasma in the compound can reach 90 days, and the slow release time of the growth factors is longer than the release time of the growth factors in the platelet-rich plasma prepared by a high-speed centrifugation method; the compound can be used in combination with collagen, recombinant collagen, hyaluronic acid, etc. for facial filling.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. It is evident that the figures in the following description are only some embodiments of the application, from which other figures can be obtained without inventive effort for a person skilled in the art. In the drawings:
FIG. 1 is a photograph taken before the platelet rich plasma gel composition prepared in accordance with the present invention is injected into the facial lacrimal canals and apple muscle parts of a subject;
FIG. 2 is a photograph taken immediately after injection of the platelet rich plasma gel composition prepared in accordance with the present invention into the facial lacrimal canals and apple muscle parts of a subject;
FIG. 3 is a photograph taken 4 days after injection of the platelet rich plasma gel composition prepared in accordance with the present invention into the facial lacrimal canals and apple muscle parts of a subject.
Detailed Description
The present invention will be further described with reference to examples for the convenience of those skilled in the art.
In a first aspect, embodiments of the present invention provide a method for preparing a platelet rich plasma gel-composite for facial filling, comprising the steps of:
step 1), placing collected blood in a container under a sterile condition;
step 2), placing the container containing blood in a centrifugal machine for centrifugal separation;
step 3), after centrifugal separation, the blood in the container is divided into three layers, namely a plasma layer, a platelet layer, a leucocyte layer, a fibrin layer and a erythrocyte layer from top to bottom;
step 4), respectively extracting the plasma layer, the platelet, the leucocyte and the fibrin layer by using a syringe, heating the syringe containing the plasma layer in water bath, and cooling the plasma layer to form gel;
Step 5), mixing the gel with the platelet, the white blood cell and the fibrin layer through a medical three-way tube to obtain the platelet-rich plasma gel compound.
In one embodiment, the container is a centrifuge tube or a blood collection tube without anticoagulant.
In one embodiment, the blood is autologous venous blood of the subject.
In one embodiment, the centrifugal force of the centrifuge in step 2) is 150×g to 300×g, and the centrifugation time is 4 to 11 minutes.
In one embodiment, the centrifuge of step 2) has a centrifugal force of 200 Xg and a centrifugation time of 8 minutes.
In one embodiment, the temperature of the water bath heating of step 4) is 60-80 ℃; the heating time is 8-10 minutes.
In one embodiment, the temperature of the water bath heating of step 4) is 70 ℃; the heating time was 9 minutes.
In one embodiment, the volume ratio of the gel to the platelet, leukocyte, and fibrin layers in step 5) is 4:1 by medical tee.
In a second aspect, the embodiment of the invention also provides a use of the platelet-rich plasma gel compound prepared by the method for preparing the platelet-rich plasma gel compound for facial filling according to any one of the above aspects in medical cosmetology.
The platelet-rich plasma gel compound prepared by the method provided by the embodiment of the invention is rich in platelets, white blood cells and fibrin, can be injected into various layers of skin, and plays a role in filling; secondly, the release time of the growth factors in the platelet-rich plasma in the compound can reach 90 days, and the slow release time of the growth factors is longer than the release time of the growth factors in the platelet-rich plasma prepared by a high-speed centrifugation method; the compound can be used in combination with collagen, recombinant collagen, hyaluronic acid, etc. for facial filling.
The method of preparing the above-described platelet rich plasma gel complex for facial filling is described in further detail below in conjunction with specific examples and comparative examples.
Examples
A method of preparing a platelet rich plasma gel complex for facial filling, comprising the steps of:
Step 1), under aseptic conditions, placing 10ml of collected autologous venous blood of a American subject into a centrifuge tube;
Step 2), placing the container containing venous blood in a centrifugal machine for centrifugal separation; centrifugal force of the centrifugal machine is 200 Xg, and centrifugal time is 8 minutes;
step 3), after centrifugal separation, the blood in the container is divided into three layers, namely a plasma layer, a platelet layer, a leucocyte layer, a fibrin layer and a erythrocyte layer from top to bottom;
Step 4), respectively extracting the plasma layer, the blood platelets, the white blood cells and the fibrin layer by using a syringe, heating the syringe containing the plasma layer in a water bath at the temperature of 70 ℃ for 9 minutes, and cooling the plasma layer to form gel;
Step 5), mixing the gel with the platelet, the white blood cell and the fibrin layer according to the volume ratio of 4:1 through a medical three-way tube to obtain the platelet-rich plasma gel compound.
Injecting the prepared platelet-rich plasma gel compound into the lacrimal duct and apple muscle parts of the face of the person to be beautified; the face photograph of the person who looks after the injection (fig. 2) was taken before the operation (fig. 1), and the face photograph of the person who looks after the operation (fig. 3) was taken for 4 days after the operation as a control.
As can be seen from comparison of fig. 1,2 and 3, the skin of the lacrimal sulcus and apple muscle of the post-operation beauty subject is obviously improved; the skin at the lacrimal canal part is smooth and tender, the skin at the apple muscle part is fine and smooth and glossy, and the compactness is improved; and the postoperative lacrimal duct and apple intramuscular injection parts have no induration and are good in recovery.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (9)
1. A method of preparing a platelet rich plasma gel complex for facial filling comprising the steps of:
step 1), placing collected blood in a container under a sterile condition;
step 2), placing the container containing blood in a centrifugal machine for centrifugal separation;
step 3), after centrifugal separation, the blood in the container is divided into three layers, namely a plasma layer, a platelet layer, a leucocyte layer, a fibrin layer and a erythrocyte layer from top to bottom;
step 4), respectively extracting the plasma layer, the platelet, the leucocyte and the fibrin layer by using a syringe, heating the syringe containing the plasma layer in water bath, and cooling the plasma layer to form gel;
Step 5), mixing the gel with the platelet, the white blood cell and the fibrin layer through a medical three-way tube to obtain the platelet-rich plasma gel compound.
2. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 1, wherein: the container is a centrifuge tube or a blood collection tube without anticoagulant.
3. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 1, wherein: the blood is autologous venous blood of the person for beauty.
4. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 1, wherein: the centrifugal force of the centrifugal machine in the step 2) is 150 Xg-300 Xg, and the centrifugal time is 4-11 minutes.
5. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 4, wherein: the centrifugal force of the centrifugal machine in the step 2) is 200 Xg, and the centrifugal time is 8 minutes.
6. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 1, wherein: the temperature of the water bath heating in the step 4) is controlled to be 60-80 ℃; the heating time is 8-10 minutes.
7. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 6, wherein: the temperature of the water bath heating in the step 4) is 70 ℃; the heating time was 9 minutes.
8. The method of preparing a platelet rich plasma gel composition for facial filling according to claim 1, wherein: in the step 5), the gel, the platelets, the white blood cells and the fibrin layer are mixed by a medical three-way pipe in a volume ratio of 4:1.
9. Use of a platelet rich plasma gel complex prepared according to the method of preparation of a platelet rich plasma gel complex for facial filling of any one of claims 1 to 8 in medical cosmetology.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410204122.6A CN118161666A (en) | 2024-02-23 | 2024-02-23 | Preparation method of platelet-rich plasma gel compound for facial filling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410204122.6A CN118161666A (en) | 2024-02-23 | 2024-02-23 | Preparation method of platelet-rich plasma gel compound for facial filling |
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| Publication Number | Publication Date |
|---|---|
| CN118161666A true CN118161666A (en) | 2024-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410204122.6A Pending CN118161666A (en) | 2024-02-23 | 2024-02-23 | Preparation method of platelet-rich plasma gel compound for facial filling |
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| Country | Link |
|---|---|
| CN (1) | CN118161666A (en) |
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- 2024-02-23 CN CN202410204122.6A patent/CN118161666A/en active Pending
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