CN118150556A - Method for measuring mineralized carbon content in soil - Google Patents
Method for measuring mineralized carbon content in soil Download PDFInfo
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- CN118150556A CN118150556A CN202410566003.5A CN202410566003A CN118150556A CN 118150556 A CN118150556 A CN 118150556A CN 202410566003 A CN202410566003 A CN 202410566003A CN 118150556 A CN118150556 A CN 118150556A
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- soil
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- carbon content
- mineralizable
- sodium hydroxide
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 72
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 72
- 239000002689 soil Substances 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 64
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000010521 absorption reaction Methods 0.000 claims abstract description 33
- 239000012086 standard solution Substances 0.000 claims abstract description 24
- 238000009792 diffusion process Methods 0.000 claims abstract description 23
- 239000000243 solution Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 17
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000005303 weighing Methods 0.000 claims abstract description 11
- 230000029058 respiratory gaseous exchange Effects 0.000 claims abstract description 7
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910001626 barium chloride Inorganic materials 0.000 claims abstract description 6
- 230000007480 spreading Effects 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 238000004448 titration Methods 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 125000004432 carbon atom Chemical group C* 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000007689 inspection Methods 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 44
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 15
- 230000008569 process Effects 0.000 description 9
- 238000005259 measurement Methods 0.000 description 8
- 239000011790 ferrous sulphate Substances 0.000 description 6
- 235000003891 ferrous sulphate Nutrition 0.000 description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 6
- 230000033558 biomineral tissue development Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000012937 correction Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000004158 soil respiration Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a method for measuring mineralizable carbon content in soil, which comprises the following steps: step 1, taking a soil sample, and determining the organic carbon content in the soil sample; step 2, according to the determination result of the organic carbon content in the soil sample, using a diffusion vessel, weighing and spreading the sample in an outer chamber of the diffusion vessel, adding excessive sodium hydroxide absorption liquid into an inner chamber to absorb CO 2 generated by the respiration of the soil, and covering a cover to perform constant-temperature culture and absorption of the mineralizable carbon in the soil; and 3, after the culture is completed, taking out the diffusion vessel, adding a barium chloride solution into the sodium hydroxide absorption liquid at the first time, stirring to fully react, taking the sodium hydroxide absorption liquid, adding a phenolphthalein indicator, titrating by using a hydrochloric acid standard solution until red disappears, and calculating the volume of the consumed hydrochloric acid standard solution to obtain the mineralizable carbon content of the soil sample. After the method is optimized, the flow is simple, the operation is simple and convenient, and the batch sample analysis work requirement of the inspection and detection mechanism can be met.
Description
Technical Field
The invention relates to the technical field of mineralized carbon content measurement, in particular to a method for measuring mineralized carbon content in soil.
Background
Soil is the global second largest organic carbon pool next to the ocean, one of the largest and most mobile ecosystem carbon pools in the earth's surface system. The soil organic carbon has the functions of improving physical and chemical properties of soil, maintaining biological diversity and sustainable development, is used as a carbon source and a carbon sink in the global carbon circulation process, influences the concentration change of CO 2 in the atmosphere, and plays a very important role in the global carbon circulation. The dynamic process of the organic carbon in the soil mainly comprises two aspects, namely that the organic carbon in the soil is changed into CO 2 through mineralization, and the organic carbon in the soil is changed into a more stable part through a humification process.
In the mineralization process of the organic carbon in the soil, the part which can be decomposed and released by microorganisms becomes mineralizable carbon, and the mineralizable carbon is also called biodegradable carbon or physiological ecological index (obligate respiration rate and metabolic respiration rate), can reflect the carbon quantity of the mineralized part of the soil, and is a link of soil fertility, environmental stress, cultivation time, duration and the like. The activity of the steady state indigenous microflora can be reflected at low values and the activity of the fermenting microflora can be reflected at high values; the method can be used for widely evaluating the microbial activity of soil, is the amount of CO 2 generated per unit microbial biomass in the process of decomposing organic matters by microorganisms, and is also a measurement index for decomposing the organic matters in the soil by the microorganisms. The mineralization of the soil is closely related to the decomposition process of the soil, the influence of environmental factors or land utilization changes on the decomposition of the organic carbon of the soil can be evaluated according to the quantity and the intensity of CO 2 released by the mineralization of the organic carbon, and meanwhile, the microbial activity of the soil can be widely evaluated. The mineralizable carbon amount can be obtained by utilizing microorganisms to decompose organic substances and measuring the release amount of CO 2.
At present, the indoor detection method of mineralized carbon in soil is a static alkali liquor culture method, and the method established by the former can effectively determine the mineralized carbon content in the soil. However, this method has several drawbacks:
1. in the culture process, the absorption device is required to be repeatedly opened, so that CO 2 gas released by the mineralization of soil is easy to escape, and the measurement result is lower;
2. the culture device has complex structure and larger volume, and occupies large space of the constant temperature incubator;
3. The method has complex flow and can not meet the requirement of batch sample analysis.
Disclosure of Invention
Aiming at the defects of the existing method for detecting mineralized carbon in soil, the invention aims to provide a method for measuring the content of mineralized carbon in soil.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
There is provided a method of determining mineralizable carbon content in soil comprising the steps of:
Step 1, taking a soil sample, and determining the organic carbon content in the soil sample;
Step 2, according to the determination result of the organic carbon content in the soil sample, using a diffusion vessel, weighing and spreading the sample in an outer chamber of the diffusion vessel, adding excessive sodium hydroxide absorption liquid into an inner chamber to absorb CO 2 generated by the respiration of the soil, and covering a cover to perform constant-temperature culture and absorption of the mineralizable carbon in the soil;
And 3, after the culture is completed, taking out the diffusion vessel, adding a barium chloride solution into the sodium hydroxide absorption liquid at the first time, stirring to fully react, taking the sodium hydroxide absorption liquid, adding a phenolphthalein indicator, titrating by using a hydrochloric acid standard solution until red disappears, and calculating the volume of the consumed hydrochloric acid standard solution to obtain the mineralizable carbon content of the soil sample. The experimental procedure reaction equation is as follows:
further, in step 2, the constant temperature culture temperature was 28℃and the culture time was 5 days.
Further, in step 3, the mineralizable carbon content of the soil sample is calculated according to the following formula:
Omega PMC -mass fraction (%) of mineralizable carbon in sample;
-sodium hydroxide absorption liquid concentration (mol/L);
-hydrochloric acid standard titration solution concentration (mol/L);
V-consumption of hydrochloric acid standard titration solution volume (mL);
Molar mass (g/mol) of 3-1/4 carbon atoms;
m-weighing the sample quality (g);
k-sample moisture conversion coefficient.
The beneficial effects of the invention are as follows:
1. in the soil sample culture and absorption process, the absorption device does not need to be repeatedly opened, and the reliability of the measurement result is stronger;
2. The culture device (diffusion vessel) has small volume, simple structure and strong sealing property, and completely meets the daily detection work requirement;
3. After the method is optimized, the flow is simple, the operation is simple and convenient, and the batch sample analysis work requirement of the inspection and detection mechanism can be met.
Drawings
FIG. 1 is a schematic view of a plastic diffusion vessel according to the present invention;
FIG. 2 is a schematic diagram of a plastic diffusion vessel according to the present invention;
FIG. 3 is a schematic diagram of a plastic diffusion vessel according to the present invention.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
Example 1
1. The method of regional geochemical sample analysis, section 27, was used DZ/T0279.27-2016: determination of organic carbon content Potassium dichromate Capacity method the determination of organic carbon (Corg) in the sample was completed.
Omega Corg -mass fraction (%) of organic carbon in sample;
0.8000-concentration (mol/L) of potassium dichromate standard solution;
5.0-potassium dichromate standard solution volume (mL);
V 0 -blank experiments consumed the volume (mL) of ferrous sulfate standard solution;
V-titration of sample solution consumes the volume (mL) of ferrous sulfate standard solution;
molar mass (g/mmol) of 0.003-1/4 carbon atoms;
1.1-redox correction factor;
m-weighing the sample quality (g);
k-sample moisture conversion coefficient.
2. According to the result of organic carbon content in the sample, a plastic diffusion vessel (comprising an outer chamber, an inner chamber and a cover, wherein the air can be isolated by covering the cover), the sample is weighed and flatly paved on the outer chamber of the diffusion vessel, 10mL of sodium hydroxide absorption liquid (the concentration is adjusted according to the organic carbon content, and the concentration is 0.1-0.5 mol/L) is added into the inner chamber to absorb CO2 generated by the respiration of the soil, the cover is covered, and the constant-temperature culture and absorption of mineralized carbon of the soil are carried out, wherein the constant-temperature culture temperature is 28 ℃, and the culture time is 5 days.
3. After the completion of the culture, the diffusion vessel was taken out, 1mL of 1mol/L barium chloride solution was added to the sodium hydroxide absorption solution at the first time, and stirred to allow the reaction to proceed sufficiently, 5mL of the sodium hydroxide absorption solution was separated into a 50mL conical flask, 2 drops of phenolphthalein indicator were added, and titration was performed using 0.05mol/L hydrochloric acid standard solution until the red color disappeared. And calculating the volume of the consumed hydrochloric acid standard solution and the mineralized carbon content of the soil.
Omega PMC -mass fraction (%) of mineralizable carbon in sample;
-sodium hydroxide absorption liquid concentration (mol/L);
-hydrochloric acid standard titration solution concentration (mol/L);
V-consumption of hydrochloric acid standard titration solution volume (mL);
Molar mass (g/mol) of 3-1/4 carbon atoms;
m-weighing the sample quality (g);
k-a sample moisture conversion coefficient;
TABLE 1 soil mineralizable carbon example 1 measurement results
Example 2
1. The method of regional geochemical sample analysis, section 27, was used DZ/T0279.27-2016: determination of organic carbon content Potassium dichromate Capacity method completes determination of organic carbon (Corg) in sample,
Omega Corg -mass fraction (%) of organic carbon in sample;
0.8000-concentration (mol/L) of potassium dichromate standard solution;
5.0-potassium dichromate standard solution volume (mL);
V 0 -blank experiments consumed the volume (mL) of ferrous sulfate standard solution;
V-titration of sample solution consumes the volume (mL) of ferrous sulfate standard solution;
molar mass (g/mmol) of 0.003-1/4 carbon atoms;
1.1-redox correction factor;
m-weighing the sample quality (g);
k-a sample moisture conversion coefficient;
2. According to the result of the organic carbon content in the sample, a plastic diffusion vessel is used, the sample is weighed and flatly paved in an outer chamber of the diffusion vessel, 10mL of sodium hydroxide absorption liquid (the concentration is adjusted according to the organic carbon content, and 0.1-0.5 mol/L) is added into the inner chamber to absorb CO2 generated by the soil respiration, a cover is covered, and constant-temperature culture and absorption of the soil mineralizable carbon are carried out, wherein the constant-temperature culture temperature is 28 ℃, and the culture time is 5 days.
3. After the completion of the culture, the diffusion vessel was taken out, 1mL of 1mol/L barium chloride solution was added to the sodium hydroxide absorption solution at the first time, and stirred to allow the reaction to proceed sufficiently, 5mL of the sodium hydroxide absorption solution was separated into a 50mL conical flask, 2 drops of phenolphthalein indicator were added, and titration was performed using 0.05mol/L hydrochloric acid standard solution until the red color disappeared. And calculating the volume of the consumed hydrochloric acid standard solution and the mineralized carbon content of the soil.
Omega PMC -mass fraction (%) of mineralizable carbon in sample;
-sodium hydroxide absorption liquid concentration (mol/L);
-hydrochloric acid standard titration solution concentration (mol/L);
V-consumption of hydrochloric acid standard titration solution volume (mL);
Molar mass (g/mol) of 3-1/4 carbon atoms;
m-weighing the sample quality (g);
k-a sample moisture conversion coefficient;
TABLE 2 soil mineralizable carbon example 2 measurement results
Example 3
1. The method of regional geochemical sample analysis, section 27, was used DZ/T0279.27-2016: determination of organic carbon content Potassium dichromate Capacity method completes determination of organic carbon (Corg) in sample,
Omega Corg -mass fraction (%) of organic carbon in sample;
0.8000-concentration (mol/L) of potassium dichromate standard solution;
5.0-potassium dichromate standard solution volume (mL);
V 0 -blank experiments consumed the volume (mL) of ferrous sulfate standard solution;
V-titration of sample solution consumes the volume (mL) of ferrous sulfate standard solution;
molar mass (g/mmol) of 0.003-1/4 carbon atoms;
1.1-redox correction factor;
m-weighing the sample quality (g);
k-sample moisture conversion coefficient.
2. According to the result of the organic carbon content in the sample, a plastic diffusion vessel is used, the sample is weighed and paved on the outer chamber of the diffusion vessel, 10mL of sodium hydroxide absorption liquid (the concentration is adjusted according to the organic carbon content, and 0.1-0.5 mol/L) is added into the inner chamber to absorb CO 2 generated by the respiration of the soil, a cover is covered, and the soil mineralizable carbon is cultivated and absorbed at a constant temperature, wherein the constant temperature cultivation temperature is 28 ℃ and the cultivation time is 5 days.
3. After the completion of the culture, the diffusion vessel was taken out, 1mL of 1mol/L barium chloride solution was added to the sodium hydroxide absorption solution at the first time, and stirred to allow the reaction to proceed sufficiently, 5mL of the sodium hydroxide absorption solution was separated into a 50mL conical flask, 2 drops of phenolphthalein indicator were added, and titration was performed using 0.05mol/L hydrochloric acid standard solution until the red color disappeared. And calculating the volume of the consumed hydrochloric acid standard solution and the mineralized carbon content of the soil.
Omega PMC -mass fraction (%) of mineralizable carbon in sample;
-sodium hydroxide absorption liquid concentration (mol/L);
-hydrochloric acid standard titration solution concentration (mol/L);
V-consumption of hydrochloric acid standard titration solution volume (mL);
Molar mass (g/mol) of 3-1/4 carbon atoms;
m-weighing the sample quality (g);
k-a sample moisture conversion coefficient;
TABLE 3 soil mineralizable carbon example 3 measurement results
In the soil sample culture and absorption process, the absorption device does not need to be repeatedly opened, and the reliability of the measurement result is stronger; the diffusion vessel has small volume, simple structure and strong sealing property, and completely meets the daily detection work requirement; after the method is optimized, the flow is simple, the operation is simple and convenient, and the batch sample analysis work requirement of the inspection and detection mechanism can be met.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (1)
1. A method for determining the mineralizable carbon content in soil, comprising the steps of:
Step 1, taking a soil sample, and determining the organic carbon content in the soil sample;
Step 2, according to the determination result of the organic carbon content in the soil sample, using a diffusion vessel, weighing and spreading the sample in an outer chamber of the diffusion vessel, adding excessive sodium hydroxide absorption liquid into an inner chamber to absorb CO 2 generated by the respiration of the soil, and covering a cover to perform constant-temperature culture and absorption of the mineralizable carbon in the soil;
Step 3, after the culture is completed, taking out the diffusion vessel, adding a barium chloride solution into a sodium hydroxide absorption liquid at the first time, stirring to fully react, taking the sodium hydroxide absorption liquid, adding a phenolphthalein indicator, titrating by using a hydrochloric acid standard solution until red disappears, and calculating the volume of the consumed hydrochloric acid standard solution to obtain the mineralizable carbon content of the soil sample;
In the step 2, the constant temperature culture temperature is 28 ℃ and the culture time is 5 days;
In step 3, the mineralizable carbon content of the soil sample is calculated according to the following formula:
；
Omega PMC -mass fraction (%) of mineralizable carbon in sample;
-sodium hydroxide absorption liquid concentration (mol/L);
-hydrochloric acid standard titration solution concentration (mol/L);
V-consumption of hydrochloric acid standard titration solution volume (mL);
Molar mass (g/mol) of 3-1/4 carbon atoms;
m-weighing the sample quality (g);
k-sample moisture conversion coefficient.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502564A (en) * | 2014-12-13 | 2015-04-08 | 齐齐哈尔大学 | Soil parameter experiment method |
| CN209167135U (en) * | 2018-11-14 | 2019-07-26 | 长治市农产品质量安全检验监测中心 | The extraction element of hydrolyzable nitrogen in a kind of soil |
| CN216082497U (en) * | 2021-08-04 | 2022-03-18 | 南京林业大学 | Diffusion dish for soil alkaline hydrolysis nitrogen determination |
| CN116879207A (en) * | 2023-08-11 | 2023-10-13 | 中国地质科学院岩溶地质研究所 | Method for determining organic matters in soil |
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- 2024-05-09 CN CN202410566003.5A patent/CN118150556A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502564A (en) * | 2014-12-13 | 2015-04-08 | 齐齐哈尔大学 | Soil parameter experiment method |
| CN209167135U (en) * | 2018-11-14 | 2019-07-26 | 长治市农产品质量安全检验监测中心 | The extraction element of hydrolyzable nitrogen in a kind of soil |
| CN216082497U (en) * | 2021-08-04 | 2022-03-18 | 南京林业大学 | Diffusion dish for soil alkaline hydrolysis nitrogen determination |
| CN116879207A (en) * | 2023-08-11 | 2023-10-13 | 中国地质科学院岩溶地质研究所 | Method for determining organic matters in soil |
Non-Patent Citations (2)
| Title |
|---|
| 曾巧云: "《环境土壤学实验教程》", 30 November 2022, 中国农业大学出版社, pages: 64 - 66 * |
| 杨新玲: "《分析化学》", 28 February 2022, 中国农业大学出版社, pages: 77 - 78 * |
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