CN118141997A - Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method - Google Patents
Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method Download PDFInfo
- Publication number
- CN118141997A CN118141997A CN202410241884.3A CN202410241884A CN118141997A CN 118141997 A CN118141997 A CN 118141997A CN 202410241884 A CN202410241884 A CN 202410241884A CN 118141997 A CN118141997 A CN 118141997A
- Authority
- CN
- China
- Prior art keywords
- silk
- tussah
- mulberry
- magnesium alloy
- fibroin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 52
- 240000000249 Morus alba Species 0.000 title claims abstract description 49
- 108010022355 Fibroins Proteins 0.000 title claims abstract description 41
- 229910000861 Mg alloy Inorganic materials 0.000 title claims abstract description 40
- 230000002792 vascular Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000000576 coating method Methods 0.000 claims abstract description 22
- 239000011248 coating agent Substances 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- BYEAHWXPCBROCE-UHFFFAOYSA-N 1,1,1,3,3,3-hexafluoropropan-2-ol Chemical compound FC(F)(F)C(O)C(F)(F)F BYEAHWXPCBROCE-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 241000255789 Bombyx mori Species 0.000 claims description 25
- 238000009835 boiling Methods 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims 1
- 238000005260 corrosion Methods 0.000 abstract description 17
- 230000007797 corrosion Effects 0.000 abstract description 15
- 230000010261 cell growth Effects 0.000 abstract description 5
- IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical group NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 abstract description 3
- 102000006495 integrins Human genes 0.000 abstract description 2
- 108010044426 integrins Proteins 0.000 abstract description 2
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 5
- 108010087230 Sincalide Proteins 0.000 description 4
- 238000010609 cell counting kit-8 assay Methods 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 241000218231 Moraceae Species 0.000 description 3
- 238000001516 cell proliferation assay Methods 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical group [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 206010053648 Vascular occlusion Diseases 0.000 description 1
- 206010057469 Vascular stenosis Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229920006237 degradable polymer Polymers 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000010339 dilation Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000001453 impedance spectrum Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 208000037803 restenosis Diseases 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000021331 vascular occlusion disease Diseases 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a magnesium alloy vascular stent coated with tussah mulberry blended fibroin and a preparation method thereof, and relates to the technical field of biomedical materials. The device comprises the following steps: degumming, dissolving, dialyzing, freeze-drying tussah and mulberry cocoons to prepare a sponge body, mixing the tussah silk and the mulberry silk sponge body according to the mass ratio of 7:3 to 3:7, dissolving in hexafluoroisopropanol to prepare a silk fibroin solution with the concentration of 100g L ‑1, and finally coating the silk fibroin solution on the surface of a magnesium alloy bracket and performing ethanol soaking post-treatment. In the invention, tussah fibroin is rich in arginine-glycine-aspartic acid sequences, can be specifically combined with various integrins, and can obviously promote the adhesion and growth of cells. Meanwhile, the addition of the mulberry silk protein increases the compactness of the coating, so that the stent has good corrosion resistance in a body fluid environment. The magnesium alloy vascular stent coated with the two fibroin has the advantages that the biocompatibility and the corrosion resistance are enhanced at the same time, and the magnesium alloy vascular stent has potential of clinical application.
Description
Technical Field
The invention relates to the technical field of biological instruments, in particular to a magnesium alloy vascular stent coated with tussah mulberry blended fibroin and a preparation method thereof.
Background
Vascular stents, as an advanced medical device, are specifically designed to restore or improve vascular patency, and particularly play an important role in treating vascular stenosis or occlusion. Such stents are typically implanted by coronary intervention (PCI) surgery, introduced through an arterial access catheter, delivered precisely to the stenosed or occluded region of the vessel, and expanded using balloon dilation techniques. Compared with the traditional open surgery, the PCI surgery has the characteristics of short recovery time and low complication risk.
The magnesium alloy stent can be gradually degraded in the body without long-term retention because the degradation product is magnesium ions, namely microelements necessary for human bodies, thereby reducing the risks of thrombosis and vascular restenosis. In mechanical property, the magnesium alloy stent is superior to a plurality of degradable polymer materials, can provide necessary supporting force without increasing the thickness of the stent, is beneficial to endothelialization of blood vessels and reduces vascular injury. However, magnesium alloys have insufficient corrosion resistance, may cause rapid corrosion and hydrogen generation at the initial stage of implantation, initiate inflammatory reaction, and have insufficient biocompatibility to support good adhesion and growth of cells on their surfaces.
Disclosure of Invention
The invention aims to provide a magnesium alloy vascular stent coated with tussah mulberry blended fibroin and a preparation method thereof, so as to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a preparation method of a magnesium alloy vascular stent coated with tussah mulberry blended fibroin comprises the following steps:
Step one: placing silkworm cocoons into 0.02M NaHCO 3 solution, boiling for 60 minutes, washing and drying the degummed silkworm cocoons with deionized water, dissolving the dried degummed silkworm silk in 9.3M lithium bromide solution at 40 ℃ for 3 hours, dialyzing the silkworm cocoons in deionized water for 3 days, and freeze-drying to obtain a silkworm silk sponge;
Step two: placing tussah cocoons in 0.02M Na 2CO3 solution, boiling for 30min, washing, repeating degumming for three times, stirring dried degummed tussah silk and molten Ca (NO 3)2·4H2 O (mass ratio of 1:35) at 100deg.C for 3h, dialyzing, and lyophilizing to obtain tussah silk sponge;
Step three: mixing tussah silk and sponge of mulberry silk according to a mass ratio of 7:3 to 3:7, and dissolving with hexafluoroisopropanol to obtain a silk fibroin solution with a concentration of 50 to 150g L -1;
Step four: and (3) coating the silk fibroin solution obtained in the step (III) on the surface of the magnesium alloy vascular stent, and soaking the silk fibroin solution in ethanol for post-treatment.
Based on the technical scheme, the invention also provides the following optional technical schemes:
In one alternative: and in the third step, the mass ratio of the tussah silk to the sponge of the mulberry silk is 7:3.
In one alternative: and in the third step, the mass ratio of the tussah silk to the sponge of the mulberry silk is 5:5.
In one alternative: and in the third step, the mass ratio of the tussah silk to the sponge of the mulberry silk is 3:7.
In one alternative: tussah silk and melted Ca (NO 3)2·4H2 O mass ratio is 1:35) in the second step.
The magnesium alloy vascular stent coated with the tussah mulberry blended fibroin is prepared by adopting any preparation method.
Compared with the prior art, the invention has the following beneficial effects:
The tussah fibroin disclosed by the invention is rich in arginine-glycine-aspartic acid sequence, and the sequence can be specifically combined with various integrins, so that the adhesion and growth of cells on the surface of a biological material are obviously promoted. Although tussah fibroin has great application potential in the biomedical field, no product is applied to the magnesium alloy vascular stent as a coating at present. The tussah fibroin and the mulberry fibroin are innovatively mixed in hexafluoroisopropanol solvent to form a novel coating, and the novel coating is coated on the surface of the magnesium alloy vascular stent. The coating aims at remarkably improving the surface biocompatibility of the stent, brings new breakthrough for clinical application of the vascular stent, and is expected to improve the treatment effect and rehabilitation process of patients.
Drawings
FIG. 1 is a graph of test data for testing corrosion resistance of a magnesium alloy vascular stent using an electrochemical workstation. a, a graph is an open circuit potential curve; b is a potentiodynamic polarization curve; c is the Nyquist plot; and d, an equivalent circuit model.
Fig. 2 shows the biocompatibility data obtained from the L929 cell assay: panel a is a cell fluorescent staining chart; b and c are the relative fluorescence intensities and relative fluorescence areas calculated from the fit of graph a; panel d shows the cell activity data obtained from CCK-8 cell proliferation assay.
Fig. 3 is a schematic structural view of a magnesium alloy vascular stent according to the present invention.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent.
Example 1 (7 tussah silk: 3 mulberry silk)
As shown in fig. 1-2, a preparation method of a magnesium alloy vascular stent coated with tussah mulberry blended fibroin comprises the following steps:
Step one: placing silkworm cocoons into 0.02M NaHCO 3 solution, boiling for 60 minutes, washing and drying the degummed silkworm cocoons with deionized water, dissolving the dried degummed silkworm silk in 9.3M lithium bromide solution at 40 ℃ for 3 hours, dialyzing the silkworm cocoons in deionized water for 3 days, and freeze-drying to obtain a silkworm silk sponge;
Step two: placing tussah cocoons in 0.02M Na 2CO3 solution, boiling for 30min, washing, repeating degumming for three times, stirring dried degummed tussah silk and molten Ca (NO 3)2·4H2 O (mass ratio of 1:35) at 100deg.C for 3h, dialyzing, and lyophilizing to obtain tussah silk sponge;
Step three: mixing tussah silk and sponge of mulberry silk according to a mass ratio of 7:3, and dissolving with hexafluoroisopropanol to obtain 100g L -1 silk fibroin solution;
step four: coating the silk fibroin solution obtained in the third step on the surface of the magnesium alloy vascular stent, and soaking the silk fibroin solution in ethanol for post-treatment;
Corrosion resistance: FIG. 1 is test data of corrosion resistance of a magnesium alloy vascular stent using an electrochemical workstation, and 7 tussah silk: 3 mulberry silk in example 1 is a red line named 7 tussah: 3 mulberry in FIG. 1. The open circuit potential in fig. 1a and the corrosion potential and current density obtained by fitting the polarization curve (fig. 1 b) are listed in table 1; the data obtained by fitting the nyquist plot (fig. 1 c) to the equivalent circuit are presented in table 2;
Fig. 1: (a) open circuit potential curves for different fibroin coatings; (b) potentiodynamic polarization curves of different fibroin coatings; (c) nyquist plots of different fibroin coatings; (d) an equivalent circuit model;
Table 1: open Circuit Potential (OCP), corrosion potential (E corr), and current density (I corr) values for the magnesium alloy vascular stent samples;
Table 2: the data obtained by fitting impedance spectrum through an equivalent circuit, wherein R s is the resistance of electrolyte, R 1 and Q 1 respectively represent the resistance and capacitance of an outer coating, R ct and Q dl represent the resistance and double-layer capacitance of interface charge transfer, and the R ct value is an important parameter reflecting the corrosion resistance of the surface of the coating, namely the equivalent resistance;
the data in Table 1 shows that the self-corrosion current (I corr) of 7 tussah: 3 mulberry is reduced relative to bare magnesium alloy vascular stents and pure tussah silk; the data in Table 2 shows that the equivalent resistance value (R ct = 8098 omega) of 7 tussah: 3 mulberry is significantly greater than that of the bare magnesium alloy vascular stent (R ct = 810.1 omega), and the resistance of the coating itself also reaches 14132 omega. Example 1 can effectively enhance the corrosion resistance of a magnesium alloy vascular stent.
The biocompatibility is shown in fig. 2;
Cells cultured in 7 tussah 3 mulberry coating extract for 12 hours were stained for actin-check ratio (FIG. 2 a), blue fluorescence for nuclear staining and green fluorescence for actin staining. The fluorescent-stained image of example 1 is marked in the pink frame in fig. 2a. The data in FIGS. 2b and 2c also show that the relative fluorescence intensity and relative fluorescence area of 7 tussah: 3 mulberry is greater than that of the control and pure mulberry silk groups, and that the CCK-8 cell proliferation assay results also show that the cell activity of example 1 is significantly improved relative to the control, compared to the control and pure mulberry silk groups. The embodiment 1 can effectively promote proliferation, differentiation and growth of cells.
Example 2 (5 tussah silk: 5 mulberry silk)
A preparation method of a magnesium alloy vascular stent coated with tussah mulberry blended fibroin comprises the following steps:
Step one: placing silkworm cocoons into 0.02M NaHCO 3 solution, boiling for 60 minutes, washing and drying the degummed silkworm cocoons with deionized water, dissolving the dried degummed silkworm silk in 9.3M lithium bromide solution at 40 ℃ for 3 hours, dialyzing the silkworm cocoons in deionized water for 3 days, and freeze-drying to obtain a silkworm silk sponge;
Step two: placing tussah cocoons in 0.02M Na 2CO3 solution, boiling for 30min, washing, repeating degumming for three times, stirring dried degummed tussah silk and molten Ca (NO 3)2·4H2 O (mass ratio of 1:35) at 100deg.C for 3h, dialyzing, and lyophilizing to obtain tussah silk sponge;
Step three: mixing tussah silk and sponge of mulberry silk in a mass ratio of 5:5, and dissolving with hexafluoroisopropanol to obtain 100g L -1 silk fibroin solution;
Step four: and (3) coating the silk fibroin solution obtained in the step (III) on the surface of the magnesium alloy vascular stent, and soaking the silk fibroin solution in ethanol for post-treatment.
Corrosion resistance:
The data in Table 1 show that the self-corrosion current of 5 tussah to 5 mulberry is significantly smaller than that of the bare magnesium alloy vascular stent; the data in Table 2 shows that the equivalent resistance value (R ct = 9732 omega) of 5 tussah: 5 mulberry is significantly greater than that of the bare magnesium alloy vascular stent (R ct = 810.1 omega), and the resistance of the coating itself is as high as 17191 omega. Example 2 can effectively enhance the corrosion resistance of the magnesium alloy vascular stent.
Biocompatibility:
Cells cultured in the 5 tussah: 5 mulberry coating extract group for 12 hours were subjected to actin-check specific staining, blue fluorescence was stained for nuclei, and green fluorescence was stained for actin. The fluorescent-stained image of example 2 is marked in the blue box in fig. 2 a. Compared with the comparison group and the pure mulberry silk, the cell number of the embodiment 1 is obviously more, the data in the fig. 2b and the fig. 2c also show that the relative fluorescence intensity and the relative fluorescence area of the 5 tussah: 5 mulberry are larger than those of the comparison group and the pure mulberry silk group, and the CCK-8 cell proliferation experimental result shows that the cell activity of the 5 tussah: 5 mulberry is obviously improved relative to the comparison group. The embodiment 2 can effectively promote proliferation, differentiation and growth of cells.
Example 3 (3 tussah silk: 7 mulberry silk)
A preparation method of a magnesium alloy vascular stent coated with tussah mulberry blended fibroin comprises the following steps:
Step one: placing silkworm cocoons into 0.02M NaHCO 3 solution, boiling for 60 minutes, washing and drying the degummed silkworm cocoons with deionized water, dissolving the dried degummed silkworm silk in 9.3M lithium bromide solution at 40 ℃ for 3 hours, dialyzing the silkworm cocoons in deionized water for 3 days, and freeze-drying to obtain a silkworm silk sponge;
Step two: placing tussah cocoons in 0.02M Na 2CO3 solution, boiling for 30min, washing, repeating degumming for three times, stirring dried degummed tussah silk and molten Ca (NO 3)2·4H2 O (mass ratio of 1:35) at 100deg.C for 3h, dialyzing, and lyophilizing to obtain tussah silk sponge;
Step three: mixing tussah silk and sponge of mulberry silk according to a mass ratio of 3:7, and dissolving with hexafluoroisopropanol to obtain 100g L -1 silk fibroin solution;
Step four: and (3) coating the silk fibroin solution obtained in the step (III) on the surface of the magnesium alloy vascular stent, and soaking the silk fibroin solution in ethanol for post-treatment.
Corrosion resistance:
The ratio of 3 tussah silk to 7 mulberries silk in example 3 is a green line designated 3 tussah to 7 mulberries in FIG. 1, and the data obtained by fitting calculation are shown in tables 1 and 2. The data in Table 1 shows that the self-etching current of 3 tussah: 7 mulberry is minimal compared to the other groups, only 2.15X10 -6A cm-2; the data in Table 2 shows that the equivalent resistance of 3 tussah: 7 mulberries (R ct = 19144 omega) is maximum, with the resistance of the coating itself being even higher than 28551 omega. Example 2 is superior in reducing corrosion rate of magnesium alloy vascular stents, and can effectively prolong the service time of the stent in the blood vessel.
Biocompatibility:
Cells cultured for 12 hours in the 3 tussah: 7 mulberry coating extract group were stained for actin-check ratio, blue fluorescence was stained for nuclei, and green fluorescence was stained for actin. The fluorescent-stained image of example 3 is marked in the green box in fig. 2 a. The relative fluorescence intensity (fig. 2 b) and the relative fluorescence area (fig. 2 c) obtained by fitting the fluorescence staining images (fig. 2 a) are more intuitively compared with the influence of different ratios on the proliferation differentiation and the growth of the cells. Wherein each item of data of example 3 (green part of bar graph) is highest. The CCK-8 cell proliferation assay of FIG. 2d shows that 3 tussah: 7 mulberry has the highest cell activity. Example 3 retains the high density and structural integrity of mulberry silk while utilizing the RGD sequence of tussah silk, effectively promoting cell proliferation.
The foregoing is merely specific embodiments of the disclosure, but the protection scope of the disclosure is not limited thereto, and any person skilled in the art can easily think about changes or substitutions within the technical scope of the disclosure, and it is intended to cover the scope of the disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (6)
1. The preparation method of the magnesium alloy vascular stent coated with the tussah mulberry blended fibroin is characterized by comprising the following steps of:
Step one: placing silkworm cocoons into 0.02M NaHCO 3 solution, boiling for 60 minutes, washing and drying the degummed silkworm cocoons with deionized water, dissolving the dried degummed silkworm silk in 9.3M lithium bromide solution at 40 ℃ for 3 hours, dialyzing the silkworm cocoons in deionized water for 3 days, and freeze-drying to obtain a silkworm silk sponge;
Step two: placing tussah cocoons in 0.02M Na 2CO3 solution, boiling for 30min, washing, repeating degumming for three times, stirring dried degummed tussah silk and molten Ca (NO 3)2·4H2 O (mass ratio of 1:35) at 100deg.C for 3h, dialyzing, and lyophilizing to obtain tussah silk sponge;
Step three: mixing tussah silk and sponge of mulberry silk according to a mass ratio of 7:3 to 3:7, and dissolving with hexafluoroisopropanol to obtain a silk fibroin solution with a concentration of 50 to 150g L -1;
Step four: and (3) coating the silk fibroin solution obtained in the step (III) on the surface of the magnesium alloy vascular stent, and soaking the silk fibroin solution in ethanol for post-treatment.
2. The magnesium alloy vascular stent coated with tussah silk blended fibroin according to claim 1, wherein the mass ratio of tussah silk to the sponge of mulberry silk in the third step is 7:3.
3. The magnesium alloy vascular stent coated with tussah silk blended fibroin according to claim 1, wherein the mass ratio of tussah silk to the sponge of mulberry silk in the third step is 5:5.
4. The magnesium alloy vascular stent coated with tussah silk blended fibroin according to claim 1, wherein the mass ratio of tussah silk to the sponge of mulberry silk in the third step is 3:7.
5. The magnesium alloy vascular stent coated with tussah silk protein blend according to claim 1, wherein the mass ratio of tussah silk to molten Ca (NO 3)2·4H2 O) in the second step is 1:35.
6. A magnesium alloy vascular stent coated with tussah mulberry blended fibroin, which is characterized by being prepared by the preparation method of any one of claims 1-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410241884.3A CN118141997A (en) | 2024-03-04 | 2024-03-04 | Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410241884.3A CN118141997A (en) | 2024-03-04 | 2024-03-04 | Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118141997A true CN118141997A (en) | 2024-06-07 |
Family
ID=91294347
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410241884.3A Pending CN118141997A (en) | 2024-03-04 | 2024-03-04 | Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118141997A (en) |
-
2024
- 2024-03-04 CN CN202410241884.3A patent/CN118141997A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| El-Kassaby et al. | Urethral stricture repair with an off-the-shelf collagen matrix | |
| CN104212998B (en) | Zn-Mg zinc alloy and preparation method and application thereof | |
| CN101549170B (en) | Human body absorbable blood vessel support and its manufacturing method | |
| CN107456601B (en) | Zn-Cu series zinc alloy and preparation method and application thereof | |
| CN102008751A (en) | Biodegradable stent composite material and preparation method thereof | |
| CN114159197B (en) | Degradable biomedical magnesium alloy drug-eluting intravascular stent and preparation method thereof | |
| CN108823476A (en) | The controllable Mg-Zn-Sn system magnesium alloy and its preparation method and application of degradation rate | |
| CN113106295A (en) | Degradable biomedical material Zn-Si-X series zinc alloy and preparation method thereof | |
| CN108815589A (en) | A kind of medical degradable zinc-containing alloy intravascular stent product | |
| CN115089773A (en) | Intravascular stent bionic coating, intravascular stent and preparation method thereof | |
| Du et al. | Characterization of a Bioresorbable Magnesium‐Reinforced PLA‐Integrated GTR/GBR Membrane as Dental Applications | |
| CN201572353U (en) | Human body absorbable intravascular stent | |
| CN118141997A (en) | Magnesium alloy vascular stent coated with tussah mulberry blended fibroin and preparation method | |
| CN106606806B (en) | A kind of Zn-Mg1Ca system kirsite and the preparation method and application thereof | |
| CN111676390B (en) | Zn-Ga alloy, preparation method and application thereof | |
| CN101502696A (en) | Medical apparatus carrying gene and/or medicament and preparation method thereof | |
| EP3395971B1 (en) | Alloy material and application thereof | |
| CN100579588C (en) | Intravascular adult stem cell rack for preventing and treating angiostenosis and preparation method thereof | |
| CN111603615B (en) | Controllable degradable high-strength magnesium-based composite stent composite coating and preparation method thereof | |
| CN109609951A (en) | A kind of magnesium alloy preparation method of surface covering BSA-MMT biological composite coating | |
| CN115627512A (en) | Biomedical magnesium alloy composite coating and preparation method thereof | |
| CN108560036B (en) | Surface-carried Zn2+Magnesium alloy material and preparation method and application thereof | |
| KR20220001031A (en) | Anti-bacterial coating method for medical implant | |
| CN105169479A (en) | Preparation method of externally constructed tissue engineering trachea matrix | |
| CN116407687A (en) | Surface modified porous iron-based bracket and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination |