CN118109282B - Batch test method and device for bacterial drug resistance - Google Patents
Batch test method and device for bacterial drug resistance Download PDFInfo
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- CN118109282B CN118109282B CN202410520436.7A CN202410520436A CN118109282B CN 118109282 B CN118109282 B CN 118109282B CN 202410520436 A CN202410520436 A CN 202410520436A CN 118109282 B CN118109282 B CN 118109282B
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- 206010059866 Drug resistance Diseases 0.000 title claims abstract description 23
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 19
- 238000010998 test method Methods 0.000 title abstract description 9
- 239000003814 drug Substances 0.000 claims abstract description 217
- 239000007788 liquid Substances 0.000 claims abstract description 95
- 238000005507 spraying Methods 0.000 claims abstract description 66
- 238000001514 detection method Methods 0.000 claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- 230000003115 biocidal effect Effects 0.000 claims abstract description 15
- 229940079593 drug Drugs 0.000 claims abstract description 12
- 239000007921 spray Substances 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 239000003638 chemical reducing agent Substances 0.000 claims description 34
- 238000012360 testing method Methods 0.000 claims description 33
- 230000005540 biological transmission Effects 0.000 claims description 27
- 230000007246 mechanism Effects 0.000 claims description 25
- 230000009467 reduction Effects 0.000 claims description 18
- 206010034133 Pathogen resistance Diseases 0.000 claims description 12
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 239000003570 air Substances 0.000 description 26
- 238000010586 diagram Methods 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000012984 antibiotic solution Substances 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000009635 antibiotic susceptibility testing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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Abstract
The invention discloses a bacterial drug resistance batch test method and device, and belongs to the technical field of bacterial drug resistance detection; the device comprises a spraying component; the medicine spraying assembly comprises a medicine supplying box and a water supplying box which are arranged on the box body, a shielding pipe which is arranged in a sliding manner and can float is sleeved outside the medicine inlet short pipe, the other end of the discharge pipe is provided with a medicine spraying hole which can uniformly mix medicine liquid and spray the medicine liquid onto paper sheets, and a rubber film with holes is fixed in the medicine spraying hole; the test method comprises the following steps: s1, placing paper sheets, S2 adding medicines, water, S3 intermittently supplying the paper sheets with bacteria, S4 spraying medicines on the paper sheets, and S5 standing and observing. The invention can not only spray the antibiotic liquid medicine with different concentrations on the paper sheet, but also monitor the drug resistance of bacteria in the environment of the antibiotic liquid medicine with different concentrations, and can ensure that the antibiotic liquid medicine is uniformly sprayed on the paper sheet.
Description
Technical Field
The invention relates to the technical field of bacterial drug resistance detection, in particular to a bacterial drug resistance batch test method and device.
Background
Bacterial resistance, also known as drug resistance, refers to the tolerance of bacteria to the action of antibacterial agents, which once developed, significantly reduces the chemotherapeutic action of the agent. Resistance can be classified into acquired resistance and natural resistance according to the cause of occurrence.
Naturally occurring pathogens, such as a strain of bacteria, may also present natural resistance. When antibiotics are used for a long time, most sensitive strains are continuously killed, and drug-resistant strains are propagated in a large quantity to replace the sensitive strains, so that the drug-resistant rate of bacteria to the drug is continuously increased. In order to maintain the effectiveness of the antibiotic, its rational use should be appreciated. There are many methods for testing bacterial resistance, a paper sheet diffusion method is a common antibiotic susceptibility test method, and the susceptibility and resistance of bacteria to antibiotics are reflected by observing the size of a bacteriostasis circle.
The prior art discloses a bacterial drug resistance change testing device with the application number of CN202311784726.4 and a testing method thereof, wherein the testing device comprises a case, a top cover is arranged at the upper end of the case, the top cover is hinged on the case, a testing mechanism is arranged in the case, the testing mechanism comprises a horizontally arranged turntable, a circular groove for placing an agar plate is arranged on the turntable, and a paper pasting component, a drug adding component and a monitoring component are respectively arranged around the axial direction of the turntable.
The above prior art has the disadvantage that it is not possible to detect the resistance of bacteria using different concentrations of antibiotics; the uniformity of the liquid medicine sprayed on the paper sheet is poor, and further improvement is needed; therefore, the application provides a bacterial drug resistance batch test method and device.
Disclosure of Invention
The invention aims to solve the technical problems and provides a bacterial drug resistance batch test method and device.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a bacterial drug resistance batch test device, includes the box, the rotation is connected with the detection dish in the box, the upper end of detection dish is equipped with a plurality of detection grooves, be equipped with the scraps of paper that contain the bacterium in the detection groove;
A spraying component; the medicine spraying assembly comprises a medicine supplying box and a water supplying box which are arranged on a box body, a piston cylinder is arranged on the box body, a movable piston is connected in a sliding manner in the piston cylinder, a medicine inlet pipe, a water outlet pipe and an air inlet pipe are arranged on the piston cylinder, a medicine inlet short pipe which is arranged vertically is arranged at the upper end of the medicine inlet pipe, a plurality of medicine inlet holes are formed in the medicine inlet short pipe, a shielding pipe which is arranged in a sliding manner and can float is sleeved outside the medicine inlet short pipe, a medicine spraying pipe which can uniformly mix medicine liquid and spray the medicine liquid onto paper sheets is arranged at the other end of the water outlet pipe, the paper sheets are driven to rotate, a plurality of medicine spraying holes are formed in the bottom of the medicine spraying pipe, and a rubber film with holes is fixed in the medicine spraying holes.
Preferably, the device further comprises a driving mechanism for driving the detection disc to intermittently rotate so that the detection groove is opposite to the medicine spraying tube, the driving mechanism comprises a reduction gearbox arranged at the upper end of the box body, a motor fixed with one shaft end of the reduction gearbox is arranged on the reduction gearbox, a transmission rod is fixed at the other shaft end of the reduction gearbox, an incomplete gear is fixed on the transmission rod, a rotating rod is coaxially and fixedly connected onto the detection disc, the rotating rod penetrates through the box body and is in rotary connection with the same, and a transmission gear meshed with the incomplete gear is fixed on the rotating rod.
Preferably, the device further comprises a transmission mechanism for driving the movable piston to reciprocate, wherein the transmission mechanism comprises a short rod fixed at the upper end of the incomplete gear, the upper end of the short rod is fixedly connected with a circular plate, the circular plate is eccentrically hinged and connected with a connecting rod, and the connecting rod is hinged and connected with the movable piston.
Preferably, the device further comprises a mixing mechanism for connecting the discharge pipe and the medicine spraying pipe together and mixing the medicine liquid, the mixing mechanism comprises a supporting plate fixed in the box body, a rotating sleeve which is arranged in a rotating mode is arranged on the supporting plate in a penetrating mode up and down, the inside of the rotating sleeve is rectangular, the rectangular sleeve is connected in a sliding mode in the rotating sleeve, a reducer pipe connected with the discharge pipe is arranged in the rectangular sleeve in a sliding mode, the medicine spraying pipe is connected with the reducer pipe, a movable gear is mounted on the reducer pipe, and a rack plate which moves synchronously with the movable piston is meshed on the movable gear.
Preferably, the outer wall fixedly connected with solid fixed ring of reducing pipe, gu fixed ring's upper end is fixed with the spring, the other end and the rotation cover bottom fixed connection of spring, the spring cover is in the outside of reducing pipe.
Preferably, a hose is mounted on the discharge pipe, a conveying pipe is mounted on the hose, a rotary joint is mounted on the conveying pipe, the rotary joint is connected with the reducer pipe, and the inner diameter of the rotary joint is smaller than that of the reducer pipe.
Preferably, the upper end of box runs through and is equipped with logical groove, it is equipped with the connecting rod that is connected with removal piston, rack board to run through in the groove to run through, fixedly connected with installation pole on the connecting rod, fixedly connected with and the wedge that the top slides in the box offsets on the installation pole, wedge sets up with the conveyer pipe relatively.
Preferably, the rack plate rack comprises a rack plate, and is characterized by further comprising a guiding mechanism for guiding the rack plate, wherein the guiding mechanism comprises a telescopic rod fixed on the rack plate, the telescopic rod is fixedly connected with the inner wall of the box body, a guiding wheel is rotatably installed on the supporting plate, and the guiding wheel is propped against the rack plate.
Preferably, the medicine inlet pipe, the water inlet pipe and the air inlet pipe are provided with inlet check valves, and the discharge pipe is provided with discharge check valves.
The invention also discloses a bacterial drug resistance batch test method, which comprises the following steps:
S1, placing paper sheets: placing paper sheets with bacteria in the detection groove, and closing the box door;
S2, adding medicines and water: respectively adding a proper amount of antibiotic liquid and clear water into the medicine supply tank and the water supply tank;
s3, intermittently supplying paper sheets with bacteria: the motor works to enable the detection groove to be positioned right below the medicine spraying pipe;
S4, spraying the medicine on the paper sheet; the motor works to drive the circular plate, the connecting rod and the movable piston to move, so that the liquid medicine can be uniformly mixed and sprayed on the paper sheets;
S5, standing and observing after all paper sheets are sprayed with the liquid medicine.
Compared with the prior art, the invention has the beneficial effects that:
1. When the movable piston moves towards the motor, air in the medicine feeding box, the water feeding box and the outside can be sucked into the piston cylinder through the medicine feeding pipe, the water inlet pipe and the air inlet pipe respectively, the mixture of air, water and liquid medicine is arranged in the piston cylinder, the air is arranged on the upper side, the liquid is arranged on the lower side, and preparation is made for cleaning residual liquid medicine subsequently.
2. The liquid medicine and water enter into the piston cylinder through the medicine inlet pipe and the water inlet pipe, the flow between the medicine and the water realizes the first-step mixing of the liquid medicine, and the inner diameter of the reducer pipe is larger than the output diameter of the rotary joint, and the movement of the piston is moved, so that the liquid medicine flows rapidly, and the liquid medicine is conveyed into the reducer pipe through the rotary joint to be in a spraying and scattering state, so that the liquid medicine can be mixed in the second step, and the uniform mixing of the liquid medicine can be ensured.
3. The antibiotic liquid medicine can be uniformly sprayed on the paper sheets with bacteria, so that the antibiotic liquid medicine can be uniformly sprayed on the paper sheets with bacteria, the change of antibacterial circles caused by different amounts of the antibiotic liquid medicine and diffusion permeation of the paper sheets is greatly reduced, the accuracy of a monitoring image is improved, and the accuracy of a test result is improved.
4. The medicine spraying pipe moves down to the detection groove, namely when the medicine spraying pipe sprays medicine, the medicine spraying pipe is positioned in the detection groove, the medicine liquid can not be sprayed outside the detection groove, the medicine liquid is wasted, and part of the medicine liquid is positioned in the detection groove.
5. When the bottom plane of the wedge-shaped block is propped against the conveying pipe, the medicine spraying pipe is propped against the test paper and extrudes the test paper, so that the medicine spraying pipe can drive the test paper to rotate when rotating along with the reducer pipe, and the medicine liquid in the detection groove is more uniformly distributed on the paper sheet.
6. When the liquid medicine is positioned at the lower side, the liquid medicine is firstly discharged through the discharge pipe and finally the air is discharged when the movable piston moves, so that the residual liquid medicine in the discharge pipe, the hose, the conveying pipe, the reducer pipe and the medicine spraying pipe can be purged after the air is finally discharged, the residual liquid medicine is reduced as far as possible, and the influence on the concentration of the liquid medicine at the lower side after conveying and mixing is reduced.
7. When the air flows to the part with smaller inner diameter at the lower side, the flow speed of the air can be increased, so that residual liquid medicine in the medicine spraying pipe can be effectively discharged, the drop of the liquid medicine is prevented from falling on the detection disc, and finally the air is blown onto the paper sheet, so that the liquid medicine on the paper sheet is further dispersed.
8. Because the liquid medicine in the medicine supply box is reduced, under the effect of shielding pipe, it moves down along with the liquid level, can shelter from the medicine inlet, when carrying the liquid medicine to the piston section of thick bamboo again, because the medicine inlet quantity in medicine inlet reduces, lead to the liquid medicine proportion and last time different in the piston section of thick bamboo that enter into, repeat above operation, so can spray the liquid medicine of different concentration to the detection inslot of difference, so can detect the drug resistance of bacterium under the different concentration.
In conclusion, the invention can spray the antibiotic liquid medicine with different concentrations on the paper sheet, can monitor the drug resistance of bacteria in the environment of the antibiotic liquid medicine with different concentrations, and can ensure that the antibiotic liquid medicine is uniformly sprayed on the paper sheet.
Drawings
FIG. 1 is a schematic diagram of a batch test device for bacterial resistance according to the present invention;
FIG. 2 is a side view of a bacterial resistance batch test device according to the present invention;
FIG. 3 is a schematic diagram of a moving piston in a bacterial resistance batch test device according to the present invention;
FIG. 4 is a rear view of a piston cylinder in a bacterial resistance batch test device according to the present invention;
FIG. 5 is a schematic diagram of the reducer in the batch test device for bacterial resistance according to the present invention;
FIG. 6 is a schematic view of a rectangular sleeve in a bacterial resistance batch test device according to the present invention;
FIG. 7 is a schematic diagram showing the disassembly of rectangular sleeves in a bacterial resistance batch test device according to the present invention;
FIG. 8 is a schematic diagram of the structure of a spray tube in a bacterial resistance batch test device according to the present invention;
Fig. 9 is a schematic diagram of the inside of a drug supply box in the bacterial drug resistance batch test device according to the present invention.
In the figure: 1 box, 2 box doors, 3 reduction boxes, 4 motors, 5 transmission rods, 6 incomplete gears, 7 through grooves, 8 rotating rods, 9 transmission gears, 10 circular plates, 11 piston cylinders, 12 connecting rods, 13 medicine supply boxes, 14 water supply boxes, 15 medicine inlet pipes, 16 water inlet pipes, 17 discharge pipes, 18 air inlet pipes, 19 detection discs, 20 detection grooves, 21 reducing pipes, 22 short rods, 23 medicine spraying pipes, 24 connecting rods, 25 fixed rings, 26 springs, 27 rotating sleeves, 28 telescopic rods, 29 rack plates, 30 moving gears, 31 hoses, 32 conveying pipes, 33 rotating joints, 34 mounting rods, 35 wedge blocks, 36 medicine spraying holes, 37 rubber films, 38 rectangular sleeves, 39 medicine inlet short pipes, 40 shielding pipes, 41 medicine inlet holes, 42 moving pistons, 43 supporting plates, 44 guide wheels, 45 inlet check valves and 46 discharge check valves.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
Referring to fig. 1-9, a bacterial drug resistance batch testing device comprises a box body 1, wherein a box door 2 is connected to the box body 1 in a cross manner, and the box door 2 is made of glass material for observation; the box body 1 is rotatably connected with a detection disc 19, a plurality of detection grooves 20 are formed in the upper end of the detection disc 19, paper sheets containing bacteria are arranged in the detection grooves 20, and the paper sheets containing bacteria are placed manually;
The device also comprises a driving mechanism for driving the detection disc 19 to intermittently rotate so that the detection groove 20 is opposite to the medicine spraying pipe 23, wherein the driving mechanism comprises a reduction gearbox 3 arranged at the upper end of the box body 1, the reduction gearbox 3 can be a worm gear or the like, the device is not limited to the worm gear, the reduction gearbox is more in the market, a proper reduction gearbox 3 can be selected, and the reduction gearbox 3 is in the prior art.
The motor 4 fixed with one shaft end of the reduction gearbox 3 is arranged on the reduction gearbox 3, the transmission rod 5 is fixed with the other shaft end of the reduction gearbox 3, the incomplete gear 6 is fixed on the transmission rod 5, the detection disc 19 is coaxially and fixedly connected with the rotating rod 8, the rotating rod 8 penetrates through the gearbox body 1 and is rotationally connected with the same, the transmission gear 9 meshed with the incomplete gear 6 is fixed on the rotating rod 8, and the incomplete gear 6 is shown in figures 1-4.
A spraying component; the medicine spraying assembly comprises a medicine supplying box 13 and a water supplying box 14 which are arranged on the box body 1, a piston cylinder 11 is arranged on the box body 1, a bracket is arranged on the box body 1, the piston cylinder 11 is arranged on the bracket and can support the piston cylinder 11, wherein the bracket is not shown in the figure;
The piston cylinder 11 is connected with the movable piston 42 in a sliding way, and further comprises a transmission mechanism for driving the movable piston 42 to reciprocate, wherein the transmission mechanism comprises a short rod 22 fixed at the upper end of the incomplete gear 6, the upper end of the short rod 22 is fixedly connected with a circular plate 10, the circular plate 10 is connected with a connecting rod 12 in an eccentric hinged manner, and the connecting rod 12 is connected with the movable piston 42 in a hinged manner;
The medicine inlet pipe 15, the water inlet pipe 16, the discharge pipe 17 and the air inlet pipe 18 are arranged on the piston cylinder 11, the medicine inlet pipe 15, the water inlet pipe 16 and the air inlet pipe 18 are provided with the one-way inlet valve 45, and the one-way inlet valve 45 only allows external fluid to enter the piston cylinder 11; the discharge pipe 17 is provided with a discharge check valve 46, the discharge check valve 46 only allows the fluid to be discharged into the discharge pipe 17 through the piston cylinder 11, and the discharge pipe 17 is arranged at the lower side of the piston cylinder 11;
The upper end of the medicine feeding pipe 15 is provided with a medicine feeding short pipe 39 which is vertically arranged, the medicine feeding short pipe 39 is provided with a plurality of medicine feeding holes 41, a shielding pipe 40 which is arranged in a sliding way and can float is sleeved outside the medicine feeding short pipe 39, the shielding pipe 40 can be attached to medicine liquid, and a balancing weight is arranged on the shielding pipe 40, so that when the liquid level of the medicine liquid moves downwards, the shielding pipe 40 can also move downwards, and the medicine liquid in the medicine feeding box 13 can not cause the shielding pipe 40 to separate from the medicine feeding short pipe 39 due to buoyancy; wherein the upper end of the medicine inlet short tube 39 is sealed.
Still include the discharge pipe 17, spout the mixing mechanism that the medicine pipe 23 linked together and mixed to the liquid medicine, mixing mechanism is including fixing the backup pad 43 in box 1, backup pad 43 runs through from top to bottom and is equipped with the rotation cover 27 that rotates the setting, the inside of rotation cover 27 is rectangular shape, sliding connection has rectangular cover 38 in the rotation cover 27, rectangular cover 38 sliding connection is in rotation cover 27, the outer wall fixedly connected with solid fixed ring 25 of reducing pipe 21, gu fixed ring 25's upper end is fixed with spring 26, the other end and the rotation cover 27 bottom fixed connection of spring 26, the spring 26 cover is in the outside of reducing pipe 21, utilize spring 26 to be used for the reduction of reducing pipe 21.
The rectangular sleeve 38 is internally provided with a reducer pipe 21 connected with the discharge pipe 17, a hose 31 is arranged on the discharge pipe 17, a conveying pipe 32 is arranged on the hose 31, a rotary joint 33 is arranged on the conveying pipe 32, the rotary joint 33 is connected with the reducer pipe 21, the inner diameter of the rotary joint 33 is smaller than that of the reducer pipe 21, the upper end of the reducer pipe 21 is plugged, and a hole connected with the rotary joint 33 is reserved, so that the rotary joint 33 can be connected with the reducer pipe 21, and the inner diameter of the rotary joint 33 is smaller than that of the reducer pipe 21;
The medicine spraying pipe 23 is connected with the reducer pipe 21, the reducer pipe 21 is provided with a movable gear 30, the movable gear 30 is meshed with a rack plate 29 which moves synchronously with a movable piston 42, the upper end of the box body 1 is provided with a through groove 7 in a penetrating way, a connecting rod 24 connected with the movable piston 42 and the rack plate 29 is provided in the through groove 7 in a penetrating way, the connecting rod 24 is fixedly connected with a mounting rod 34, the mounting rod 34 is fixedly connected with a wedge block 35 which slides against the inner top of the box body 1, and the wedge block 35 is arranged opposite to the conveying pipe 32.
The rack plate 29 is guided by the guide mechanism, the guide mechanism comprises a telescopic rod 28 fixed on the rack plate 29, the telescopic rod 28 is fixedly connected with the inner wall of the box body 1, a guide wheel 44 is rotatably arranged on a supporting plate 43, and the guide wheel 44 is propped against the rack plate 29 to ensure stable movement of the rack plate 29;
The other end of the discharge pipe 17 is provided with a medicine spraying pipe 23 which can uniformly mix medicine liquid and spray the medicine liquid onto paper sheets, the paper sheets can be driven to rotate, the bottom of the medicine spraying pipe 23 is provided with a plurality of medicine spraying holes 36, a rubber film 37 with holes is fixed in the medicine spraying holes 36, when the pressure in the medicine spraying pipe 23 is increased, the medicine liquid can prop open the rubber film 37 with holes, then the medicine liquid is sprayed, and therefore medicine liquid spraying is dispersed.
When the invention is used, a worker places paper sheets with bacteria in the detection groove 20, closes the box door 2 and opens the motor 4;
The motor 4 works to reduce the speed of the transmission rod 5 through the reduction gearbox 3, so that the rotation speed of the transmission rod 5 is reduced, the transmission rod 5 rotates to drive the incomplete gear 6 to rotate, the incomplete gear 6 rotates to drive the transmission gear 9 to rotate, the incomplete gear 6 lacks gear teeth, the incomplete gear 6 can drive the transmission gear 9 to intermittently rotate, the transmission gear 9 rotates to drive the rotation rod 8 and the detection plate 19 to intermittently rotate, and when the incomplete gear 6 is not meshed with the transmission gear 9, the other detection groove 20 is positioned under the medicine spraying pipe 23.
The incomplete gear 6 rotates to drive the short rod 22 to rotate, and then drives the circular plate 10 to rotate, the circular plate 10 rotates to drive the connecting rod 12 and the movable piston 42 to move, and the movable piston 42 moves reciprocally, when the movable piston 42 moves towards the direction of the motor 4, the air of the medicine supply box 13, the water supply box 14 and the outside can be sucked into the piston cylinder 11 through the medicine inlet pipe 15, the water inlet pipe 16 and the air inlet pipe 18 respectively, wherein the antibiotic medicine liquid enters into the medicine inlet pipe 15 through the medicine inlet hole 41 and the medicine inlet short pipe 39; the pipe diameter of the air inlet pipe 18 is smaller than that of the medicine inlet pipe 15 and the water inlet pipe 16, so that air can not be sucked into the piston cylinder 11 (the proper air inlet pipe 18 can be selected for a plurality of times), and therefore, the mixture of air, water and liquid medicine is arranged in the piston cylinder 11, the air is positioned on the upper side, and the liquid is positioned on the lower side;
the liquid medicine and water enter the piston cylinder 11 through the medicine inlet pipe 15 and the water inlet pipe 16, and the flow between the medicine and the water realizes the first step of mixing of the liquid medicine;
The above is when the incomplete gear 6 is meshed with the transmission gear 9.
When the incomplete gear 6 is not meshed with the transmission gear 9, when the movable piston 42 moves away from the motor 4, the medicine water mixture can be discharged through the discharge pipe 17 and conveyed into the reducer pipe 21 through the hose 31, the conveying pipe 32 and the rotary joint 33, and as the inner diameter of the reducer pipe 21 is larger than the diameter output by the rotary joint 33 and the movable piston 42 moves, medicine liquid rapidly flows, so that the medicine liquid is conveyed into the reducer pipe 21 through the rotary joint 33 to be in a spraying and scattering state, the medicine liquid can be mixed in the second step, and the uniform mixing of the medicine liquid can be ensured;
The finally mixed liquid medicine flows into the medicine spraying pipe 23, the pressure in the medicine spraying pipe 23 is increased, the liquid medicine can prop open the rubber film 37 with holes, and then the liquid medicine is sprayed, so that the liquid medicine is sprayed and dispersed and sprayed on paper sheets.
The movable piston 42 moves to drive the connecting rod 24 to move, the connecting rod 24 drives the rack plate 29 to move, the rack plate 29 moves to drive the movable gear 30 and the reducer 21 to rotate, the reducer 21 rotates to drive the medicine spraying pipe 23 to rotate, so that the medicine spraying pipe 23 can be sprayed onto a piece of paper with bacteria when rotating, the antibiotic liquid can be uniformly sprayed onto the piece of paper with bacteria, the uniformity of antibiotic solution on each place of the piece of paper can be realized, the change of an antibacterial circle caused by the difference between the amount of the antibiotic solution and diffusion permeation on each place of the piece of paper is greatly reduced, the accuracy of a monitoring image is improved, and the accuracy of a test result is improved.
The rack plate 29 moves to drive the mounting rod 34 to move, the mounting rod 34 moves to drive the wedge block 35 to move, the wedge block 35 moves to squeeze the conveying pipe 32, the conveying pipe 32 moves downwards to drive the reducer 21, the rectangular sleeve 38 and the medicine spraying pipe 23 to move downwards, and the rectangular sleeve 38 and the rotating sleeve 27 rotate and slide relatively; the medicine spraying pipe 23 moves downwards into the detection groove 20, that is, when the medicine spraying pipe 23 sprays medicine, the medicine spraying pipe 23 is positioned in the detection groove 20, so that medicine liquid is not sprayed outside the detection groove 20, the medicine liquid is wasted, and part of the medicine liquid is positioned in the detection groove 20;
when the bottom plane of the wedge 35 is propped against the conveying pipe 33, the medicine spraying pipe 23 is propped against the test paper and extrudes the test paper, so that when the medicine spraying pipe 23 rotates along with the reducer pipe 21, the test paper can be driven to rotate, and the medicine liquid in the detection groove 20 is more uniformly distributed on the paper sheets.
It should be noted that, since the liquid medicine is located at the lower side, when the moving piston 42 moves, the liquid medicine is discharged through the discharge pipe 17 first, and finally the air is discharged, so that the residual liquid medicine in the discharge pipe 17, the hose 31, the conveying pipe 33, the reducer pipe 21 and the medicine spraying pipe 23 can be purged after the air is finally discharged, the residual liquid medicine is reduced as much as possible, and the influence on the concentration of the liquid medicine at the next side after the liquid medicine is conveyed and mixed is reduced.
Because the reducer pipe 21 is arranged, as shown in fig. 5, when air flows to the part with smaller inner diameter at the lower side, the flow speed of the air is increased, so that the residual liquid medicine in the medicine spraying pipe 23 can be effectively discharged, the liquid medicine is prevented from dripping on the detection disc 19, and finally the air is blown to the paper sheet, so that the liquid medicine on the paper sheet is further dispersed;
Immediately after the movable piston 42 moves back, the wedge block 35 is separated from the rotary joint 33 and is reset under the action of the spring 26, the medicine spraying pipe 23 is positioned above the detection disc 19, at the moment, the incomplete gear 6 is meshed with the transmission gear 9, the detection disc 19 is driven to move, and the other detection groove 20 is positioned below the medicine spraying pipe 23;
Due to the fact that the liquid medicine in the medicine supply box 13 is reduced, under the action of the shielding pipe 40, the medicine supply box moves downwards along with the liquid level, the medicine inlet holes 41 can be shielded, when the liquid medicine is conveyed into the piston cylinder 11 again, the proportion of the liquid medicine entering the piston cylinder 11 is different from that of the liquid medicine entering the piston cylinder 11 last time due to the fact that the medicine inlet quantity of the medicine inlet holes 41 is reduced, the operation is repeated, liquid medicines with different concentrations can be sprayed into different detection grooves 20, and therefore the medicine resistance of bacteria with different concentrations can be detected.
Changes in the plurality of test slots 20 may be monitored by a camera.
The invention also discloses a bacterial drug resistance batch test method, which comprises the following steps:
S1, placing paper sheets: placing a paper sheet with bacteria in the detection groove 20, and closing the box door 2;
s2, adding medicines and water: an appropriate amount of antibiotic liquid and clear water are respectively added into the medicine feed tank 13 and the water feed tank 14;
s3, intermittently supplying paper sheets with bacteria: the motor 4 works to enable the detection groove 20 to be positioned right below the medicine spraying pipe 23;
s4, spraying the medicine on the paper sheet; the motor 4 works to drive the circular plate 10, the connecting rod 12 and the movable piston 42 to move, so that the liquid medicine can be uniformly mixed and sprayed on paper sheets;
S5, standing and observing after all paper sheets are sprayed with the liquid medicine.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (7)
1. The utility model provides a bacterial drug resistance batch test device, includes box (1), its characterized in that, box (1) swivelling joint has detection dish (19), the upper end of detection dish (19) is equipped with a plurality of detection grooves (20), be equipped with the scraps of paper that contain the bacterium in detection groove (20);
A spraying component; the medicine spraying component comprises a medicine feeding box (13) and a water supply box (14) which are arranged on a box body (1), a piston cylinder (11) is arranged on the box body (1), a movable piston (42) is connected in a sliding manner in the piston cylinder (11), a medicine feeding pipe (15), a water inlet pipe (16), a discharge pipe (17) and an air inlet pipe (18) are arranged on the piston cylinder (11), a medicine feeding short pipe (39) which is vertically arranged is arranged at the upper end of the medicine feeding pipe (15), a plurality of medicine feeding holes (41) are arranged on the medicine feeding short pipe (39), a shielding pipe (40) which is arranged in a sliding manner and can float is sleeved outside the medicine feeding short pipe (39), a medicine spraying pipe (23) which can uniformly mix medicine liquid and spray the medicine to the paper sheet, a plurality of medicine spraying holes (36) are arranged at the bottom of the medicine spraying pipe (23), a rubber film (37) with holes is fixed in the medicine spraying holes (36), a detection disc (19) is arranged at the bottom of the medicine spraying pipe (11), the medicine spraying pipe is directly opposite to a speed reducing mechanism (3) which is arranged on a driving box (3), the other shaft end of the reduction gearbox (3) is fixedly provided with a transmission rod (5), the transmission rod (5) is fixedly provided with an incomplete gear (6), the detection disc (19) is fixedly connected with a rotation rod (8) in a coaxial and fixedly connected manner, the rotation rod (8) penetrates through the box body (1) and is rotationally connected with the box body, the rotation rod (8) is fixedly provided with a transmission gear (9) meshed with the incomplete gear (6), the reduction gearbox further comprises a transmission mechanism for driving a movable piston (42) to reciprocate, the transmission mechanism comprises a short rod (22) fixed at the upper end of the incomplete gear (6), the upper end of the short rod (22) is fixedly connected with a circular plate (10), the circular plate (10) is eccentrically and hingedly connected with a connecting rod (12), the connecting rod (12) is hinged with the movable piston (42), the reduction gearbox further comprises a mixing mechanism for connecting a discharge pipe (17) and a medicine spraying pipe (23) together and mixing medicine liquid, the mixing mechanism comprises a support plate (43) fixed in the box body (1), the support plate (43) is provided with a rectangular rotating sleeve (27) which is rotatably arranged up and down, the rectangular sleeve (27) is connected with the rectangular sleeve (27) in a sliding sleeve (38), the rectangular sleeve (38) is internally provided with a reducer pipe (21) connected with the discharge pipe (17), the medicine spraying pipe (23) is connected with the reducer pipe (21), the reducer pipe (21) is provided with a movable gear (30), and the movable gear (30) is meshed with a rack plate (29) which moves synchronously with the movable piston (42).
2. The bacterial drug resistance batch testing device according to claim 1, wherein the outer wall of the reducer pipe (21) is fixedly connected with a fixed ring (25), a spring (26) is fixed at the upper end of the fixed ring (25), the other end of the spring (26) is fixedly connected with the bottom of the rotating sleeve (27), and the spring (26) is sleeved outside the reducer pipe (21).
3. The bacterial drug resistance batch testing device according to claim 1, wherein a hose (31) is installed on the discharge pipe (17), a conveying pipe (32) is installed on the hose (31), a rotary joint (33) is installed on the conveying pipe (32), the rotary joint (33) is connected with the reducer pipe (21), and the inner diameter of the rotary joint (33) is smaller than the inner diameter of the reducer pipe (21).
4. The bacterial drug resistance batch testing device according to claim 3, wherein the upper end of the box body (1) is provided with a through groove (7) in a penetrating way, a connecting rod (24) connected with a movable piston (42) and a rack plate (29) is arranged in the through groove (7) in a penetrating way, a mounting rod (34) is fixedly connected to the connecting rod (24), a wedge block (35) sliding against the inner top of the box body (1) is fixedly connected to the mounting rod (34), and the wedge block (35) is arranged opposite to the conveying pipe (32).
5. The bacterial drug resistance batch testing device according to claim 1, further comprising a guiding mechanism for guiding the rack plate (29), wherein the guiding mechanism comprises a telescopic rod (28) fixed on the rack plate (29), the telescopic rod (28) is fixedly connected with the inner wall of the box body (1), a guiding wheel (44) is rotatably arranged on the supporting plate (43), and the guiding wheel (44) is propped against the rack plate (29).
6. The batch test device for bacterial resistance according to claim 1, wherein the drug inlet pipe (15), the water inlet pipe (16) and the air inlet pipe (18) are provided with inlet check valves (45), and the discharge pipe (17) is provided with discharge check valves (46).
7. A method for batch testing of bacterial resistance of a device according to any one of claims 1-6, comprising the steps of:
s1, placing paper sheets: placing a paper sheet with bacteria in the detection groove (20), and closing the box door (2);
s2, adding medicines and water: an appropriate amount of antibiotic liquid and clear water are respectively added into the medicine supply tank (13) and the water supply tank (14);
S3, intermittently supplying paper sheets with bacteria: the motor (4) works to enable the detection groove (20) to be positioned right below the medicine spraying pipe (23);
s4, spraying the medicine on the paper sheet; the motor (4) works to drive the circular plate (10), the connecting rod (12) and the movable piston (42) to move, so that the liquid medicine can be uniformly mixed and sprayed on paper sheets;
S5, standing and observing after all paper sheets are sprayed with the liquid medicine.
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| CN116716178A (en) * | 2023-06-13 | 2023-09-08 | 山东益生种畜禽股份有限公司 | Salmonella detection device |
| CN117721005A (en) * | 2023-12-23 | 2024-03-19 | 木蕊(上海)医药科技有限公司 | Bacterial drug resistance change testing device and testing method thereof |
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| CN216560424U (en) * | 2021-10-28 | 2022-05-17 | 济南铭域医学检验实验室有限公司 | Sample feeding device of high performance liquid chromatography-tandem mass spectrometer |
| CN117736841B (en) * | 2024-02-21 | 2024-05-03 | 青岛市动物疫病预防控制中心 | Bacterial stock solution treatment device for bacterial drug resistance detection and analysis and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116716178A (en) * | 2023-06-13 | 2023-09-08 | 山东益生种畜禽股份有限公司 | Salmonella detection device |
| CN117721005A (en) * | 2023-12-23 | 2024-03-19 | 木蕊(上海)医药科技有限公司 | Bacterial drug resistance change testing device and testing method thereof |
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