CN118108334A - Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof - Google Patents
Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof Download PDFInfo
- Publication number
- CN118108334A CN118108334A CN202311856084.4A CN202311856084A CN118108334A CN 118108334 A CN118108334 A CN 118108334A CN 202311856084 A CN202311856084 A CN 202311856084A CN 118108334 A CN118108334 A CN 118108334A
- Authority
- CN
- China
- Prior art keywords
- concentration
- stage
- reactor
- ammonia oxidation
- autotrophic denitrification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 182
- 229910052717 sulfur Inorganic materials 0.000 title claims abstract description 100
- 230000001651 autotrophic effect Effects 0.000 title claims abstract description 96
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 title claims abstract description 93
- 239000011593 sulfur Substances 0.000 title claims abstract description 93
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 91
- 230000003647 oxidation Effects 0.000 title claims abstract description 89
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 89
- 230000008878 coupling Effects 0.000 title claims abstract description 35
- 238000010168 coupling process Methods 0.000 title claims abstract description 35
- 238000005859 coupling reaction Methods 0.000 title claims abstract description 35
- 238000010276 construction Methods 0.000 title claims abstract description 23
- 239000012528 membrane Substances 0.000 title abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 76
- 230000008569 process Effects 0.000 claims abstract description 70
- 239000010802 sludge Substances 0.000 claims abstract description 60
- 239000000945 filler Substances 0.000 claims abstract description 40
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 34
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 79
- 241000894006 Bacteria Species 0.000 claims description 17
- 238000007599 discharging Methods 0.000 claims description 11
- 238000009342 intercropping Methods 0.000 claims description 10
- 239000002028 Biomass Substances 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 238000009825 accumulation Methods 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 239000010865 sewage Substances 0.000 claims description 6
- 229920005830 Polyurethane Foam Polymers 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 239000011496 polyurethane foam Substances 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- AHEWZZJEDQVLOP-UHFFFAOYSA-N monobromobimane Chemical compound BrCC1=C(C)C(=O)N2N1C(C)=C(C)C2=O AHEWZZJEDQVLOP-UHFFFAOYSA-N 0.000 claims description 3
- 239000004745 nonwoven fabric Substances 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 48
- 239000000758 substrate Substances 0.000 abstract description 12
- 230000003993 interaction Effects 0.000 abstract description 9
- 229910002651 NO3 Inorganic materials 0.000 description 11
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 7
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 7
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 7
- 239000006227 byproduct Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 241000605118 Thiobacillus Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 3
- 230000009935 nitrosation Effects 0.000 description 3
- 238000007034 nitrosation reaction Methods 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 230000010718 Oxidation Activity Effects 0.000 description 2
- 239000000370 acceptor Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000001272 nitrous oxide Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 241000480585 Candidatus Kuenenia Species 0.000 description 1
- 241001212280 Hydrogenophilaceae Species 0.000 description 1
- 241001509286 Thiobacillus denitrificans Species 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 230000023556 desulfurization Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003464 sulfur compounds Chemical class 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- AYNNSCRYTDRFCP-UHFFFAOYSA-N triazene Chemical compound NN=N AYNNSCRYTDRFCP-UHFFFAOYSA-N 0.000 description 1
- 150000004654 triazenes Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/006—Regulation methods for biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2813—Anaerobic digestion processes using anaerobic contact processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, a construction method and application thereof, comprising the following steps: inoculating mixed sludge consisting of sulfur autotrophic denitrification sludge and anaerobic ammoxidation sludge in a reactor; wherein, the MLSS of the reactor after inoculation is 1000-8000 mg/L; carrying out staged domestication on the mixed sludge to obtain domesticated sludge; and adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane. The construction method is convenient and easy to operate, and the process flow is simple. The obtained sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane solves the problem that the total nitrogen depth removal is difficult to realize in the prior art. The biological membrane can be directly inoculated in an anaerobic system reactor under proper substrate conditions, so that stable interaction of two denitrification processes is realized, the total nitrogen removal performance is enhanced, and the biological membrane has stronger practical engineering application capability.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, a construction method and application thereof.
Background
In the sewage denitrification treatment process, the prior art usually uses an anaerobic ammonia oxidation process means to remove total nitrogen in the polluted water body. The anaerobic ammoxidation utilizes nitrite and ammonium salt as substrates, and uses a small amount of nitrate as a byproduct to produce nitrogen under the anoxic condition, so that the removal process of nitrogen-containing pollutants is simplified. Compared with the traditional biological denitrification process, the anaerobic ammonia oxidation process has the advantages of high efficiency, environmental protection, high nitrogen load, no need of external organic carbon source, low sludge yield, less oxygen supply energy consumption and the like, and is considered as the most sustainable and environmentally acceptable denitrification process.
However, in the practical application process, the nitrosation process often cannot obtain an ideal control effect, and nitrate byproducts generated in the anaerobic ammonia oxidation process all result in still limited total nitrogen removal capacity.
Disclosure of Invention
The invention mainly aims to provide a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, a construction method and application thereof, and aims to solve the problems that the total nitrogen depth removal is difficult to realize in the prior art.
In order to achieve the above purpose, the invention provides a construction method of a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, comprising the following steps: inoculating mixed sludge consisting of sulfur autotrophic denitrification sludge and anaerobic ammoxidation sludge in a reactor; wherein, the MLSS of the reactor after inoculation is 1000-8000 mg/L.
And carrying out staged domestication on the mixed sludge to obtain domesticated sludge.
And adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane.
Further, the staged domestication sequentially comprises: and (3) feeding the liquid in the first stage into the reactor, staying in the reactor, discharging, and entering the next stage when the accumulation rate of NO 2 -N in the effluent liquid of the reactor is more than 50 percent.
And (3) feeding the liquid in the second stage into the reactor, staying in the reactor, discharging, and entering the next stage when the content of NO 2 -N in the effluent liquid of the reactor is less than 10 mg/L.
And (3) feeding the liquid in the third stage into the reactor, staying in the reactor, discharging, and entering the next stage when the removal rate of NH 4 -N in the effluent liquid of the reactor is more than 75 percent.
Wherein the first stage liquid, the second stage liquid and the third stage liquid all comprise a C source, an N source, an S source and microelements.
Further, NH 4-N、NO3 -N and S 2O3 -S are included in each of the first stage liquid, the second stage liquid, and the third stage liquid; in the first stage liquid, the ratio of the concentration of the NH 4 -N to the concentration of the NO 3 -N is 1:1, wherein the concentration of the NH 4 -N and the concentration of the NO 3 -N are both 40-60 mg/L; the concentration of S 2O3 -S is 60-75 mg/L.
In the second stage liquid, the concentration of the NH 4 -N is consistent with the concentration of the NH 4 -N in the first stage liquid; the ratio of the concentration of NH 4 -N to the concentration of NO 3 -N is 1:2; the concentration of S 2O3 -S is consistent with the concentration of S 2O3 -S in the first stage liquid.
In the third stage liquid, the ratio of the concentration of the NH 4 -N to the concentration of the NH 4 -N in the second stage liquid is 3:2; the concentration of the NO 3 -N is consistent with the concentration of the NO 3 -N in the second stage liquid; the ratio of the concentration of S 2O3 -S to the concentration of S 2O3 -S in the second stage liquid is 2:1.
Further, the hydraulic retention time of the sulfur autotrophic denitrification start-up stage, the anaerobic ammonia oxidation start-up stage and the sulfur autotrophic denitrification and anaerobic ammonia oxidation intercropping stage is 24h.
The first stage liquid, the second stage liquid, and the third stage liquid each have a dissolved oxygen of less than 0.2mg/L.
Further, the ratio of the biomass of the sulfur autotrophic denitrification sludge and the anaerobic ammoxidation sludge is 1:4 to 5.
Further, the abundance of the sulfur autotrophic denitrification functional bacteria in the sulfur autotrophic denitrification sludge is more than 10%; the abundance of anaerobic ammonia oxidation functional bacteria in the anaerobic ammonia oxidation sludge is more than 5%.
Further, the filling ratio of the carrier filler is 15-30%.
Further, the carrier filler comprises one or more of non-woven fabric filler, polyurethane foam filler, MBBR filler, suspension ball filler, fiber ball filler, activated carbon filler, volcanic rock filler and ceramsite filler.
The invention also provides the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane constructed by the construction method.
The invention also provides an application of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane or the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane constructed by the construction method according to any one of the above in the sewage denitrification treatment process.
The invention has the beneficial effects that:
According to the construction method of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, mixed sludge (MLSS is 1000-8000 mg/L) consisting of sulfur autotrophic denitrification sludge and anaerobic ammonia oxidation sludge is inoculated in a reactor. The mixed sludge is domesticated in stages, a sulfur autotrophic denitrification process and an anaerobic ammonia oxidation process are respectively started, and cross-intercropping of the two denitrification processes is rapidly realized, so that the domesticated sludge is obtained. And adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane. The construction method is convenient and easy to operate, and the process flow is simple.
The obtained sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane can stably provide nitrite for the anaerobic ammonia oxidation process, maintain the anaerobic ammonia oxidation activity, remove the byproduct nitrate of the anaerobic ammonia oxidation process, realize the deep removal of total nitrogen, and solve the problem that the prior art is difficult to realize the deep removal of total nitrogen. The biological membrane can be directly inoculated in any anaerobic system reactor under proper substrate conditions, can realize stable interaction of two denitrification processes of autotrophic denitrification and anaerobic ammoxidation, strengthens the total nitrogen removal performance, and has stronger practical engineering application capacity.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a graph showing the variation of the content of triazenes in the effluent of a reactor during the stage-by-stage domestication in example 1 of the present invention;
FIG. 2 is a photograph showing the construction of a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biofilm according to the embodiment 1 of the present invention;
FIG. 3 is a graph showing the analysis of the diversity of 16S microorganisms of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biofilm constructed in the embodiment 1 of the present invention;
FIG. 4 is a graph showing the denitrification efficiency of the sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation biofilm in example 2 of the present invention.
The achievement of the object, functional features and advantages of the present invention will be further described with reference to the drawings in connection with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
It should be noted that the following embodiments and features in the embodiments may be combined with each other without conflict. It is also to be understood that the terminology used in the examples of the invention is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and to which this invention belongs, and any method, apparatus, or material of the prior art similar or equivalent to the methods, apparatus, or materials described in the examples of this invention may be used to practice the invention.
Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. The test methods in the following examples, in which specific conditions are not noted, are generally conducted under conventional conditions or under conditions recommended by the respective manufacturers. The materials or reagents required in the examples below are commercially available unless otherwise specified.
In the research process, a sufficient amount of nitrous oxide (NO 2 -N) is needed to be used as a reaction substrate in the practical application process of the anaerobic ammonia oxidation process, but the nitrosation process cannot always obtain an ideal control effect in the practical application, so that the anaerobic ammonia oxidation process is easily inhibited by insufficient nitrous oxide content, and the total nitrogen removal capacity is unstable. In addition, total nitrogen depth removal cannot be achieved by means of the anaerobic ammoxidation process alone, as the process produces a certain amount of nitrogen by-product, which results in still limited total nitrogen removal capacity. That is, anaerobic ammoxidation processes require sufficient nitrite as an electron acceptor, and are often combined with short range nitrification or short range denitrification processes. However, because anaerobic ammoxidation requires a relatively fixed ratio of ammonia nitrogen (NH 4 -N) to nitrite substrate, in the practical application process, the nitrosation process often cannot obtain an ideal control effect because dissolved oxygen is difficult to control accurately.
In addition, sulfur autotrophic denitrification is an anaerobic or anoxic physiological activity taking advantage of the metabolic characteristics of denitrifying desulfurization bacteria such as thiobacillus denitrificans (Thiobacillus denitrifications) with reduced sulfur compounds as electron donors and NO 3- or NO 2- as electron acceptors. In the experimental process, the autotrophic property based on the chemical energy of the sulfur autotrophic denitrifying bacteria is found, if the sulfur autotrophic denitrifying bacteria are coupled with the anaerobic ammonia oxidation, nitrite required by the anaerobic ammonia oxidation can be stably provided, nitrate as a byproduct can be removed, and the total nitrogen removal efficiency of the system is improved. However, both the sulfur autotrophic denitrification and the anaerobic ammoxidation can utilize nitrite as a reaction substrate, and certain competition relationship exists, so that stable interaction between the two processes is difficult to realize.
In order to solve the problems that the total nitrogen depth removal and the like are difficult to realize in the prior art, the invention provides a construction method of a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological film, which comprises the following steps: inoculating mixed sludge consisting of sulfur autotrophic denitrification sludge and anaerobic ammoxidation sludge in a reactor; wherein, the MLSS of the reactor after inoculation is 1000-8000 mg/L. Specifically, when the sludge concentration (MLSS of reactor after inoculation) >8000mg/L, the reactor control is inconvenient; in addition, more dead bacteria possibly appear in the subsequent staged domestication process, more organic matters can be generated, heterotrophic bacteria propagation is promoted, and the system stability is affected. If the sludge concentration is less than 1000mg/L, the problems of poor denitrification performance, low film forming speed and the like can occur.
And (5) carrying out staged domestication on the mixed sludge to obtain the domesticated sludge. And adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane. Specifically, when the reactor containing the domesticated sludge added with the carrier filler continuously runs for more than 30 days, the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane obtained in the system can reach a relatively stable state, and is relatively compact in adhesion and not easy to fall off.
According to the construction method of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, mixed sludge (MLSS is 1000-8000 mg/L) consisting of sulfur autotrophic denitrification sludge and anaerobic ammonia oxidation sludge is inoculated in a reactor. The mixed sludge is domesticated in stages, a sulfur autotrophic denitrification process and an anaerobic ammonia oxidation process are respectively started, and cross-intercropping of the two denitrification processes is rapidly realized, so that the domesticated sludge is obtained. And adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane. The construction method is convenient and easy to operate, and the process flow is simple.
Further, the staged domestication sequentially comprises: the liquid in the first stage is fed into the reactor, is discharged after staying in the reactor, and enters the next stage after the accumulation rate of NO 2 -N in the effluent liquid of the reactor is more than 50 percent. Specifically, the stage is a sulfur autotrophic denitrification start-up stage; feeding the first-stage liquid into the reactor at one time, and discharging after a period of stay; and (3) carrying out a plurality of times of liquid inlet and discharge circulation until the accumulation rate of NO 2 -N in the effluent of the reactor is more than 50 percent, and ending the sulfur autotrophic denitrification starting stage. In this stage, the sulfur autotrophic denitrification will reduce the nitrate nitrogen (NO 3 -N) to the nitrite nitrogen (NO 2 -N) first, so the nitrite nitrogen content is the judging basis of the activity of the sulfur autotrophic denitrification process. The domestication process in the stage can rapidly start the denitrification process so as to provide a sufficient amount of nitrite nitrogen for the system to start the anaerobic ammonia oxidation process, and when the nitrite nitrogen content reaches more than 50%, the activity requirement of the sulfur autotrophic denitrification process can be met, and the next stage is entered.
And (3) feeding the liquid in the second stage into the reactor, staying in the reactor, discharging, and entering the next stage when the content of NO 2 -N in the effluent of the effluent liquid of the reactor is less than 10 mg/L. Specifically, the stage is an anaerobic ammonia oxidation start-up stage; feeding the second-stage liquid into the reactor once after the sulfur autotrophic denitrification starting stage is finished, and discharging after a period of stay; and (3) carrying out a plurality of times of liquid inlet and discharge circulation until the content of NO 2 -N in the effluent of the reactor is less than 10mg/L, and ending the anaerobic ammonia oxidation starting stage. The nitrite nitrogen accumulated during the sulfur autotrophic denitrification start-up stage is consumed by the anaerobic ammonia oxidation process occurring in the anaerobic ammonia oxidation start-up stage, and when the activity of the anaerobic ammonia oxidation process is higher, the nitrite nitrogen is reduced to a lower level, namely when the nitrite nitrogen in the effluent is below 10mg/L, the anaerobic ammonia oxidation starts to play a role stably.
And (3) feeding the liquid in the third stage into the reactor, staying in the reactor, discharging, and entering the next stage when the removal rate of NH 4 -N in the effluent of the effluent liquid of the reactor is more than 75 percent. Specifically, the stage is a sulfur autotrophic denitrification and anaerobic ammoxidation intercropping stage; feeding the liquid in the third stage into the reactor once after the anaerobic ammonia oxidation starting stage is finished, and discharging after staying for a period of time; and (3) recycling the liquid inlet and the liquid outlet for a plurality of times until the removal rate of NH 4 -N in the effluent of the reactor is more than 75 percent, and ending the sulfur autotrophic denitrification and anaerobic ammonia oxidation intercropping stage. Since the nitrous nitrogen required for the anaerobic ammoxidation reaction is provided by means of a denitrification process, the denitrification process itself consumes nitrous nitrogen and competes with the anaerobic ammoxidation process. Therefore, when the anaerobic ammonia oxidation process in the system occupies windy place in the nitrous competition, the more active anaerobic ammonia oxidation process can lead to the ammonia nitrogen (NH 4 -N) to be largely removed by the anaerobic ammonia oxidation. Conversely, if the ammonia nitrogen removal rate is lower, the denitrification is superior in competition, the anaerobic ammonia oxidation is inhibited, and the ammonia nitrogen removal rate of the system is at a lower level. Therefore, the ammonia nitrogen content is a sign of the stable interaction degree of the system. When the removal rate of ammonia nitrogen in the effluent is more than 75%, stable interaction of the two processes of the sulfur autotrophic denitrification process and the anaerobic ammonia oxidation process can be realized just in the sulfur autotrophic denitrification and anaerobic ammonia oxidation intercropping stage.
Wherein, the first stage liquid, the second stage liquid and the third stage liquid comprise a C source, an N source, an S source and microelements.
Further, NH 4-N、NO3 -N and S 2O3 -S are included in each of the first stage liquid, the second stage liquid, and the third stage liquid. Specifically, NH 4 -N and NO 3 -N can be used as substrates for sulfur autotrophic denitrification and anaerobic ammoxidation processes, and interaction of the two processes can be realized more easily by using the two components as substrates; s 2O3 -S can be used as an electron donor for denitrification to obtain a faster reaction rate.
In the first stage liquid, the ratio of NH 4 -N to NO 3 -N concentration was 1:1, and the concentration of NH 4 -N and NO 3 -N is 40-60 mg/L; the concentration of S 2O3 -S is 60-75 mg/L. Preferably, in the first stage liquid, the concentration of NH 4 -N is 50mg/L; the concentration of NO 3 -N is 50mg/L; the concentration of S 2O3 -S was 67.5mg/L.
In the second stage liquid, the concentration of NH 4 -N is consistent with the concentration of NH 4 -N in the first stage liquid; the ratio of NH 4 -N to NO 3 -N concentration is 1:2; the concentration of S 2O3 -S was consistent with the concentration of S 2O3 -S in the first stage liquid. Preferably, in the second stage liquid, the concentration of NH 4 -N is 50mg/L; the concentration of NO 3 -N is 100mg/L; the concentration of S 2O3 -S was 67.5mg/L.
In the third stage liquid, the ratio of the concentration of NH 4 -N to the concentration of NH 4 -N in the second stage liquid was 3:2; the concentration of NO 3 -N is consistent with the concentration of NO 3 -N in the second stage liquid; the ratio of S 2O3 -S to the concentration of S 2O3 -S in the second stage liquid was 2:1. preferably, in the third stage liquid, the concentration of NH 4 -N is 75mg/L; the concentration of NO 3 -N is 100mg/L; the concentration of S 2O3 -S was 135mg/L.
The three-stage domestication is carried out according to the proportion of each component, which is beneficial to realizing the interaction of the two processes of the sulfur autotrophic denitrification process and the anaerobic ammonia oxidation process. Too high or too low a ratio of the components is prone to inhibit one of the microorganisms in competition, which is detrimental to the overall reactivity.
Further, the hydraulic retention time of the sulfur autotrophic denitrification start-up stage, the anaerobic ammonia oxidation start-up stage and the sulfur autotrophic denitrification and anaerobic ammonia oxidation intercropping stage is 24h.
The dissolved oxygen of the first stage liquid, the second stage liquid and the third stage liquid is less than 0.2mg/L.
Further, the ratio of biomass of the sulfur autotrophic denitrification sludge and the anaerobic ammoxidation sludge is 1:4 to 5. The ratio of biomass of the sulfur autotrophic denitrification sludge to the anaerobic ammoxidation sludge is 1: 4-5 are optimal ranges for achieving stable interaction; a biomass ratio that does not meet this ratio range can affect the overall denitrification activity of the system or result in poor performance of certain processes.
Further, the abundance of the sulfur autotrophic denitrification functional bacteria in the sulfur autotrophic denitrification sludge is more than 10%; the abundance of anaerobic ammonia oxidation functional bacteria in the anaerobic ammonia oxidation sludge is more than 5 percent. When the abundance of the sulfur autotrophic denitrification functional bacteria or the abundance of the anaerobic ammonia oxidation functional bacteria is too low, the domestication effect can be influenced, the domestication time can be prolonged, and the cost is increased.
Further, the filling ratio of the carrier filler is 15-30%. When the filling ratio of the carrier filler is less than 15%, the obtained sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological film is less due to the limited microorganism growth space provided by a single carrier. When the loading ratio of the carrier filler is >30%, the biomass of the obtained single sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological film is low.
Further, the carrier filler comprises one or more of non-woven fabric filler, polyurethane foam filler, MBBR filler, suspension ball filler, fiber ball filler, activated carbon filler, volcanic rock filler and ceramsite filler.
The invention also provides the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane constructed by the construction method.
The invention also provides the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane or the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane constructed by the construction method according to any one of the above. Specifically, a nitrogen-containing pollutant water body (a matrix containing NH 4-N、NO3-N、S2O3 -S and KHCO 3) can be added into a reactor containing the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane to carry out sewage denitrification treatment, after the sewage treatment is carried out for 10 hours, the total nitrogen removal rate of the nitrogen-containing pollutant water body can reach more than 88%, and the denitrification efficiency of the biomembrane can reach more than 0.4mg Nh -1/h.
The sulfur autotrophic denitrifying bacteria in the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane can stably provide nitrite for the anaerobic ammonia oxidation process, maintain the anaerobic ammonia oxidation activity, remove the byproduct nitrate of the anaerobic ammonia oxidation process, realize the deep removal of total nitrogen, and solve the problem that the prior art is difficult to realize the deep removal of total nitrogen. The biological membrane can be directly inoculated in any anaerobic system reactor under proper substrate conditions, can realize stable interaction of two denitrification processes of autotrophic denitrification and anaerobic ammoxidation, strengthens the total nitrogen removal performance, and has stronger practical engineering application capacity.
For a further understanding of the present invention, an illustration is now given:
Example 1
Sulfur autotrophic denitrification sludge (the abundance 50.93% of functional bacteria) and anaerobic ammoxidation sludge (the abundance 17.37%) are mixed and inoculated into an SBR reactor with effective volume of 4L according to the biomass ratio of 1:5, and MLSS of the SBR reactor after inoculation is 4895mg/L, so that mixed sludge to be acclimatized in the reactor is obtained.
Carrying out staged domestication on the mixed sludge, wherein the water inflow NH 4-N、NO3 -N and S 2O3 -S in the first stage (sulfur autotrophic denitrification starting stage) are respectively 50mg/L, 50mg/L and 67.5mg/L for 5 days; the second stage (anaerobic ammonia oxidation starting stage) is characterized in that the inflow water NH 4-N、NO3 -N and S 2O3 -S are respectively 50mg/L, 100mg/L and 67.5mg/L, and the duration is 5 days; the NH 4-N、NO3 -N and S 2O3 -S of the water inflow (the sulfur autotrophic denitrification and anaerobic ammoxidation intercropping stage) of the third stage are 75mg/L, 100mg/L and 135mg/L respectively, and last for 9 days; the Hydraulic Retention Time (HRT) of the three stages is always kept at 24 hours, and Dissolved Oxygen (DO) is less than 0.2, so that the domesticated sludge is obtained.
Adding polyurethane foam carrier filler into an SBR reactor containing domesticated sludge, wherein the carrier filling ratio is 25%, and continuously operating for 45 days under the conditions that the inlet water NH 4-N、NO3 -N and S 2O3 -S are 75mg/L, 100mg/L and 135mg/L respectively, DO is less than 0.2, HRT=24 h and the temperature is 30+/-5 ℃ to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane.
Wherein, the change chart of the content of the triazene in the reactor effluent in the staged domestication process is shown in figure 1. The results according to FIG. 1 show that the accumulation rate of NO 2 -N in the effluent from the first stage (0-5 days) is gradually increased, which indicates that the activity of sulfur autotrophic denitrification is gradually increased in the stage. The accumulation of NO 2 -N is determined by the nature of the sulfur autotrophic denitrification process, since the reaction rate of NO 3 -N reduction to NO 2 -N is much higher than the reaction rate of NO 2 -N reduction to N 2. Thus, under conditions of insufficient S source, some NO 2 -N accumulation occurs, which provides a substrate condition for the initiation of the anaerobic ammonia oxidation process. The NO 2 -N content of the effluent in the second stage (6-10 days) gradually decreases, because after the concentration of the NO 3 -N in the inlet water is increased, the system has sufficient NO 2 -N production, and the lower S 2O3 -S content limits the consumption of NO 2 -N in the denitrification process, so that the anaerobic ammoxidation activity gradually increases, and the NH 4 -N in the outlet water continuously decreases. In the third stage (11-19 days), after the concentration of the inflow water NH 4 -N and the concentration of the inflow water S 2O3 -S are increased, the sulfur autotrophic denitrification and the anaerobic ammonia oxidation process are enhanced, the NH 4 -N removal rate is gradually increased, so that the anaerobic ammonia oxidation gradually takes the dominant position in the system, the sulfur autotrophic denitrification is mainly responsible for continuously providing NO 2 -N for the anaerobic ammonia oxidation as a reaction substrate and removing a byproduct NO 3 -N in the anaerobic ammonia oxidation process, and two denitrification microorganism intercropping systems are gradually formed.
The obtained real-time image of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane is shown in figure 2. As can be seen from FIG. 2, the microorganisms in the biofilm are uniformly attached and agglomerated and distributed throughout the carrier, indicating that the sulfur autotrophic denitrification and anammox bacteria grow in large amounts in the biofilm.
The sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane is subjected to 16S microorganism diversity analysis, and the analysis result is shown in figure 3. As can be seen from the observation of FIG. 3, the content of Candidatus _ Kuenenia (anammox) in the biological film is 6.14%, the content of both sulfur autotrophic denitrification functional bacteria of Hydrogenophilaceae (hydrogenphagostimula) and Thiobacillus (Thiobacillus) is 9.82%, and the content of both functional microorganisms is relatively rich.
Example 2
3 Constructed sulfur autotrophic denitrification coupled anaerobic ammonia oxidation biofilms were taken from the reactor of example 1, placed in a 250ml anaerobic jar, and a substrate containing 25mg/L NH 4-N、35mg/L NO3-N,75mg/L S2O3-S,50mg/L KHCO3 was added. Culturing at 30 deg.C and 120rpm with DO < 0.2mg/L for 10 hr, sampling every 2 hr for analysis and determination.
The specific denitrification efficiency is shown in FIG. 4. The results according to fig. 4 show that the biological membrane denitrification process presents obvious metabolic characteristics of sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation. The sulfur autotrophic denitrification is dominant in the initial reaction period, NO 3 -N is rapidly reduced within 0-2 h, and the content of NO 2 -N reaches the peak value. The anaerobic ammoxidation process then dominates, the levels of NO 2 -N and NH 4 -N gradually decrease, and the sulfur autotrophic denitrification continues to occur, so that NO 3 -N is always stabilized at a lower level. After 10 hours, the total nitrogen removal rate of the biological film reaches 88.79 percent, the denitrification rate of the biological film is 0.45mg Nh -1/one, and the high total nitrogen removal performance is shown.
In summary, the above embodiments of the present invention are only preferred embodiments of the present invention, and therefore, the scope of the present invention is not limited by the above embodiments, and all equivalent structural changes made by the description and the accompanying drawings under the technical concept of the present invention, or direct/indirect application in other related technical fields are included in the scope of the present invention.
Claims (10)
1. The construction method of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane is characterized by comprising the following steps:
Inoculating mixed sludge consisting of sulfur autotrophic denitrification sludge and anaerobic ammoxidation sludge in a reactor; wherein, the MLSS of the reactor after inoculation is 1000-8000 mg/L;
Carrying out staged domestication on the mixed sludge to obtain domesticated sludge;
And adding carrier filler into the reactor containing the domesticated sludge, and continuously operating for more than 30 days to obtain the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biomembrane.
2. The method of claim 1, wherein the staged domestication comprises, in order:
Feeding the liquid in the first stage into the reactor, staying in the reactor, discharging, and entering the next stage when the accumulation rate of NO 2 -N in the effluent liquid of the reactor is more than 50%;
Feeding the liquid of the second stage into the reactor, staying in the reactor, discharging, and entering the next stage when the content of NO 2 -N in the effluent liquid of the reactor is less than 10 mg/L;
Feeding the liquid in the third stage into the reactor, staying in the reactor, discharging, and entering the next stage when the removal rate of NH 4 -N in the effluent liquid of the reactor is more than 75%;
Wherein the first stage liquid, the second stage liquid and the third stage liquid all comprise a C source, an N source, an S source and microelements.
3. The method of claim 2, wherein each of the first stage liquid, the second stage liquid, and the third stage liquid includes NH 4-N、NO3 -N and S 2O3 -S therein;
In the first stage liquid, the ratio of the concentration of the NH 4 -N to the concentration of the NO 3 -N is 1:1, wherein the concentration of the NH 4 -N and the concentration of the NO 3 -N are both 40-60 mg/L; the concentration of S 2O3 -S is 60-75 mg/L;
In the second stage liquid, the concentration of the NH 4 -N is consistent with the concentration of the NH 4 -N in the first stage liquid; the ratio of the concentration of NH 4 -N to the concentration of NO 3 -N is 1:2; the concentration of S 2O3 -S is consistent with the concentration of S 2O3 -S in the first stage liquid;
In the third stage liquid, the ratio of the concentration of the NH 4 -N to the concentration of the NH 4 -N in the second stage liquid is 3:2; the concentration of the NO 3 -N is consistent with the concentration of the NO 3 -N in the second stage liquid; the ratio of the concentration of S 2O3 -S to the concentration of S 2O3 -S in the second stage liquid is 2:1.
4. The construction method according to claim 2, wherein the hydraulic retention time of the sulfur autotrophic denitrification start-up phase, the anaerobic ammonia oxidation start-up phase and the sulfur autotrophic denitrification and anaerobic ammonia oxidation intercropping phase is 24 hours;
The first stage liquid, the second stage liquid, and the third stage liquid each have a dissolved oxygen of less than 0.2mg/L.
5. The construction method according to claim 1, wherein the ratio of biomass of the sulfur autotrophic denitrification sludge and the anaerobic ammoxidation sludge is 1:4 to 5.
6. The construction method according to claim 1, wherein the abundance of sulfur autotrophic denitrification functional bacteria in the sulfur autotrophic denitrification sludge is >10%;
the abundance of anaerobic ammonia oxidation functional bacteria in the anaerobic ammonia oxidation sludge is more than 5%.
7. The method of claim 1, wherein the carrier filler is present in a loading ratio of 15 to 30%.
8. The method of claim 1, wherein the carrier filler comprises one or more of a nonwoven fabric filler, a polyurethane foam filler, an MBBR filler, a suspension ball filler, a fiber ball filler, an activated carbon filler, a volcanic rock filler, and a ceramsite filler.
9. A sulfur autotrophic denitrification coupled anaerobic ammonia oxidation biofilm constructed by the construction method of any one of claims 1-8.
10. Use of the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biofilm according to claim 9 or constructed by the construction method according to any one of claims 1 to 8 in a sewage denitrification treatment process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311856084.4A CN118108334A (en) | 2023-12-29 | 2023-12-29 | Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311856084.4A CN118108334A (en) | 2023-12-29 | 2023-12-29 | Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118108334A true CN118108334A (en) | 2024-05-31 |
Family
ID=91214695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311856084.4A Pending CN118108334A (en) | 2023-12-29 | 2023-12-29 | Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118108334A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118420119A (en) * | 2024-07-03 | 2024-08-02 | 湖南三友环保科技有限公司 | Water-jet viscose fiber composite particle, preparation method, application and sewage treatment system |
-
2023
- 2023-12-29 CN CN202311856084.4A patent/CN118108334A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118420119A (en) * | 2024-07-03 | 2024-08-02 | 湖南三友环保科技有限公司 | Water-jet viscose fiber composite particle, preparation method, application and sewage treatment system |
| CN118420119B (en) * | 2024-07-03 | 2024-09-20 | 湖南三友环保科技有限公司 | Water-jet nonwoven composite particles, preparation method, application and sewage treatment process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7438816B2 (en) | Method for treating water containing ammonium-nitrogen | |
| Zaman et al. | Simultaneous nitrification-denitrifying phosphorus removal (SNDPR) at low DO for treating carbon-limited municipal wastewater | |
| CN113087134A (en) | Device and method for quickly realizing integration of partial shortcut nitrification/anaerobic ammonia oxidation by adding hydroxylamine and combining with low sludge age control | |
| CN104787889B (en) | The method for recovering municipal sewage short distance Anammox using the micro- exposure of hypoxemia and anoxia stirring | |
| CN114230006B (en) | Method for natural enrichment of anaerobic ammonia oxidizing bacteria | |
| CN110683643A (en) | Enrichment method of anaerobic ammonium oxidation bacteria | |
| CN110921820A (en) | Method for quickly starting normal-temperature short-cut nitrification of municipal sewage by using benzethonium chloride | |
| Wang et al. | Free ammonia-free nitrous acid based partial nitrification in sequencing batch membrane aerated biofilm reactor | |
| CN118108334A (en) | Sulfur autotrophic denitrification coupling anaerobic ammonia oxidation biological membrane, construction method and application thereof | |
| Beylier et al. | 6.27-Biological nitrogen removal from domestic wastewater | |
| CN111406036A (en) | Method for treating nitrogen in wastewater by nitrosation organisms | |
| CN103224285A (en) | Rapid starting method of membrane bioreactor shortcut nitration technology | |
| CN106904732A (en) | A kind of method that membrane bioreactor quickly starts short distance nitration | |
| CN113511731B (en) | Method for improving nitrite accumulation in short-range denitrification process | |
| Kapagiannidis et al. | Comparison between UCT type and DPAO biomass phosphorus removal efficiency under aerobic and anoxic conditions | |
| JP2004305816A (en) | Nitrification method and apparatus, and waste water treatment equipment | |
| CN102992477A (en) | Non-oxygen limit starting method for nitrosoation of low-ammonia nitrogen sewage part | |
| CN108439588B (en) | Plug flow type reaction device and method for stable operation of nitrosation-anaerobic ammonia oxidation process | |
| CN107739086B (en) | Denitrification method of high-salinity wastewater | |
| CN109748394B (en) | Landfill leachate SBBR-SBR deep denitrification combined treatment process | |
| CN103011409B (en) | Method for realizing stable operation of nitrosification of domestic sewage in sequencing batch reactor (SBR) by using intermittent aeration | |
| JP5199794B2 (en) | Nitrogen-containing organic wastewater treatment method | |
| CN112408594A (en) | Method for strengthening culture of anaerobic ammonium oxidation bacteria and improving bacterial activity by using ferrous sulfide | |
| CN115557606B (en) | Sulfur autotrophic denitrification and anaerobic ammonia oxidation coupling denitrification method | |
| CN116143283B (en) | Device and method for realizing denitrification and dephosphorization of urban domestic sewage by integrated PD/A coupling denitrification and dephosphorization quick start |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Country or region after: China Address after: Yuelu District City, Hunan province 410000 Changsha Lushan Road No. 932 Applicant after: CENTRAL SOUTH University Applicant after: Hunan Xinyuan Environmental Technology Group Co.,Ltd. Address before: Yuelu District City, Hunan province 410000 Changsha Lushan Road No. 932 Applicant before: CENTRAL SOUTH University Country or region before: China Applicant before: Hunan Xinyuan Environmental Technology Co.,Ltd. |