CN118106053A - Multi-target detection device and method suitable for microfluidic chip - Google Patents

Abstract

The invention belongs to the technical field of reagent detection, and in particular relates to a multi-target detection device and a detection method thereof, which are applicable to a microfluidic chip, wherein the multi-target detection device comprises: the heating component controls the temperature in the microfluidic chip component, and the reagent to be detected in the microfluidic chip component is amplified; the reaction mixing component intermittently attracts magnetic beads in the microfluidic chip component to move through magnetic force, and a reagent to be detected in the microfluidic chip component is mixed with the fluorescent marking reagent; the fluorescence detection component sends out detection light to the microfluidic chip component, and the corresponding detection result is output through the observation component; according to the invention, through the non-contact arrangement of the heating component and the microfluidic chip component, the reagent to be detected can be rapidly centrifuged and heated for amplification, the reaction mixing component can uniformly mix the reagent to be detected and the fluorescent marking reagent, and meanwhile, the fluorescent detection component and the observation component are matched to realize rapid multi-target detection and automation of the detection process, so that the device has the advantages of small volume, low cost, simple structure, convenience in operation and easiness in carrying.

Description

Multi-target detection device and method suitable for microfluidic chip

Technical Field

The invention belongs to the technical field of reagent detection, and particularly relates to a multi-target detection device and a detection method thereof, which are applicable to a microfluidic chip.

Background

The microfluidic chip uses centrifugal force as fluid driving force to realize detection and analysis of multiple indexes of the same sample to be detected.

At present, a microfluidic chip in a multi-target detection device needs to be subjected to contact heating to realize amplification, a reagent to be detected in the microfluidic chip needs to enter a reaction cavity under the centrifugal action, functional modules are dispersed, the whole detection period is long, the reagent to be detected in the microfluidic chip needs to be taken out and put into a test tube to be mixed with a fluorescent marking reagent after reamplification is finished, the mixing is not uniform enough, the detection precision can be influenced, and the whole detection process is complex.

Therefore, there is a need to develop a new multi-target detection device and detection method suitable for microfluidic chips to solve the above problems.

Disclosure of Invention

The invention aims to provide a multi-target detection device and a detection method thereof, which are applicable to a microfluidic chip.

In order to solve the above technical problems, the present invention provides a multi-target detection device suitable for a microfluidic chip, comprising: the device comprises a shell assembly, a heating assembly, a centrifugal assembly, a fluorescence detection assembly, a microfluidic chip assembly, a reaction mixing assembly and an observation assembly; the heating component, the centrifugal component, the fluorescence detection component, the microfluidic chip component and the reaction mixing component are arranged in the shell component, the heating component, the centrifugal component and the fluorescence detection component are positioned below the microfluidic chip component, the centrifugal component is connected with the microfluidic chip component, and the reaction mixing component is positioned above the microfluidic chip component; the observation assembly is arranged on the outer side of the shell assembly and is positioned on the light path of the fluorescence detection assembly; the centrifugal component drives the microfluidic chip component to rotate so as to centrifuge the reagent to be detected in the microfluidic chip component to a corresponding position; the heating component controls the temperature in the microfluidic chip component so as to amplify the reagent to be detected in the microfluidic chip component; the reaction mixing assembly intermittently attracts magnetic beads in the microfluidic chip assembly to move through magnetic force so as to mix a reagent to be detected in the microfluidic chip assembly with a fluorescent marking reagent; and the fluorescence detection component sends out detection light to the microfluidic chip component, and the corresponding detection result is output through the observation component.

Specifically, the housing assembly includes: a main housing and a housing cover; the shell cover is hinged to the top of the main shell; a groove I is formed in the top of the main shell and is used for accommodating the heating component, the centrifugal component, the fluorescence detection component and the microfluidic chip component; a second groove is formed in the shell cover and used for accommodating the reaction mixing component; the observation assembly is movably arranged on the shell cover, and a first window communicated with the second groove is formed in the shell cover.

Specifically, the microfluidic chip assembly includes: a microfluidic chip cover plate, a microfluidic chip intermediate layer and a microfluidic chip bottom plate; the microfluidic chip cover plate, the microfluidic chip intermediate layer and the microfluidic chip bottom plate are sequentially stacked from top to bottom; a sample adding hole is formed in the microfluidic chip cover plate, and a detection hole is formed in the middle layer of the microfluidic chip; the microfluidic chip cover plate and the microfluidic chip bottom plate are solid and are communicated with the detection holes at the positions corresponding to the detection holes.

Specifically, an air hole is formed in the microfluidic chip cover plate and communicated with the detection hole.

Specifically, the detection hole is divided into a plurality of sub-holes; the sub-holes comprise a quantitative hole, a first connecting hole, a buffer hole, a second connecting hole and a reaction hole; the quantitative hole, the first connecting hole, the buffer hole, the second connecting hole and the reaction hole respectively form a quantitative cavity, a first channel, a buffer cavity, a second channel and a reaction cavity with the microfluidic chip cover plate and the microfluidic chip bottom plate; any one of the quantifying cavities is communicated with the adjacent quantifying cavity through the second channel, and the quantifying cavity is communicated with the reaction cavity through the first channel and the buffer cavity.

Specifically, the reaction mixing component comprises: the device comprises a mixed driving piece, a fixed frame, a plurality of mixed magnets and a plurality of magnetic beads; the mixing driving piece is positioned in the second groove and is connected with the shell cover; the fixing frame is connected with the mixed driving piece, and each mixed magnet is arranged on the fixing frame; each magnetic bead is respectively positioned in the corresponding reaction cavity.

Specifically, the fluorescence detection assembly includes: the LED light emitting substrate, the light homogenizing plate, the first optical filter and the second optical filter; the LED light emitting substrate, the light homogenizing plate and the first optical filter are sequentially arranged below the micro-fluidic chip assembly from bottom to top, the second optical filter is arranged in the first window, and the micro-fluidic chip assembly is positioned between the first optical filter and the second optical filter.

Specifically, the viewing assembly includes: a magnifying glass; the magnifying glass is arranged on the shell component, and the observation direction of the magnifying glass faces to the second optical filter.

Specifically, the magnifying glass is movably connected with the shell component through the support and the damping rotating shaft.

In another aspect, the present invention provides a detection method using the multi-target detection device suitable for a microfluidic chip, including: driving the micro-fluidic chip assembly to rotate through the centrifugal assembly so as to centrifuge the reagent to be detected in the micro-fluidic chip assembly to a corresponding position; controlling the temperature in the microfluidic chip assembly through the heating assembly so as to amplify the reagent to be detected in the microfluidic chip assembly; intermittently attracting magnetic beads in the microfluidic chip assembly to move through magnetic force by the reaction mixing assembly so as to mix the reagent to be detected in the microfluidic chip assembly with the fluorescent marking reagent; and detecting light rays are sent out to the microfluidic chip assembly through the fluorescence detection assembly, and corresponding detection results are output through the observation assembly.

The invention has the beneficial effects that the heating component and the microfluidic chip component are arranged in a non-contact way, so that the centrifugal and heating amplification of the reagent to be detected can be realized quickly, the reaction mixing component can uniformly mix the reagent to be detected and the fluorescent marking reagent, and meanwhile, the fluorescent detection component and the observation component are matched to realize the automation of the quick multi-target detection and detection process, and the invention has the advantages of small volume, low cost, simple structure, convenient operation and easy carrying.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

In order to make the above objects, features and advantages of the present invention more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a perspective view of the right front side of a multi-target detection device of the present invention; FIG. 2 is a rear view of the multi-target detection device of the present invention; fig. 3 is a structural perspective view of a microfluidic chip assembly of the present invention; FIG. 4 is a top view of a microfluidic chip cover plate of the present invention; FIG. 5 is a top view of an intermediate layer of a microfluidic chip of the present invention; FIG. 6 is a top view of a microfluidic chip backplane of the present invention; FIG. 7 is a perspective view of the left front side of the heating assembly of the present invention; FIG. 8 is a perspective view of the left front side of the fluorescence detection assembly of the present invention; fig. 9 is a front view of a microfluidic chip assembly of the present invention; FIG. 10 is a perspective view of the right front side of the cover of the present invention; FIG. 11 is a perspective view of the right front side of the interior of the main housing of the present invention; FIG. 12 is a front view of the interior of the main housing of the present invention; fig. 13 is a top view of a microfluidic chip assembly of the present invention; FIG. 14 is a perspective view of the right front side of the hybrid drive within the housing cover of the present invention; FIG. 15 is a perspective view of the right front side of the cover of the present invention; FIG. 16 is a front view of the interior of the cover of the present invention; FIG. 17 is a top view of the main housing of the present invention; FIG. 18 is a front view of the interior of the main housing of the present invention; FIG. 19 is a top view of a main control board of the present invention; FIG. 20 is a front view of the main control board of the present invention; FIG. 21 is a front view of the bottom mounting plate of the present invention; FIG. 22 is a right front perspective view of the battery compartment housing and its interior of the present invention; FIG. 23 is a perspective view of the right front side of the heating assembly of the present invention; fig. 24 is a top view of a heating zone of the present invention.

In the figure:

1. A sample adding hole; 2. bonding the positioning holes; 3. air holes; 4. a heat insulation tank; 5. a second channel; 6. a first fixing hole; 7. a buffer chamber; 8. a dosing chamber; 9. a first channel; 10. a reaction chamber; 11. a microfluidic chip cover plate; 12. an intermediate layer of the microfluidic chip; 13. a microfluidic chip base plate; 14. positioning holes I; 15. a cover; 16. a main housing; 17. a bottom mounting plate; 18. a battery compartment housing; 19. a battery compartment bottom plate; 20. a power switch; 21. Type-C power interface; 22. a transparent PMMA protective shell; 23. a magnifying glass; 24. a bracket; 25. rotating the hinge; 26. fixing a hinge; 27. a first optical filter; 28. damping the rotating shaft; 29. a second optical filter; 30. a fixing frame; 31. a hybrid magnet; 32. a hybrid drive; 33. a hall sensor; 34. PCB heating circuit board; 35. a core seat body; 36. a core seat bottom plate; 37. heating the amplification key; 38. a key indication circuit board; 39. a main control board; 40. a light homogenizing plate; 41. a centrifugal motor; 42. a first wire bundle hole; 43. an LED light-emitting substrate; 44. a shaft sleeve; 45. detecting a position magnet; 46. a second wire bundle hole; 47. a first mounting hole; 48. a heating position magnet; 49. closing the first magnet; 50. a second fixing hole; 51. closing the second magnet; 52. a lithium battery module; 53. a second mounting hole; 54. a fixing hole III; 55. a mounting groove; 56. a wire bundle hole III; 57. a support column; 58. a fixing hole IV; 59. a shaft hole site; 60. a wire bundle hole IV; 61. a bottom plate magnet; 62. double-color LED indicator lamps; 63. a single-color LED indicator light; 64. a key cap; 65. a fluorescence detection key; 66. a battery compartment magnet; 67. a microfluidic chip magnet; 68. a microfluidic chip assembly; 69. a heating resistor; 70. a temperature measuring chip; 71. binding posts; 72. and a fixing hole five.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Embodiment 1, in this embodiment, as shown in fig. 1 to 24, the present embodiment provides a multi-target detection device suitable for a microfluidic chip, which includes: a housing assembly, a heating assembly, a centrifugation assembly, a fluorescence detection assembly, a microfluidic chip assembly 68, a reaction mixing assembly, and an observation assembly; the heating component, the centrifugal component, the fluorescence detection component, the microfluidic chip component 68 and the reaction mixing component are arranged in the shell component, the heating component, the centrifugal component and the fluorescence detection component are positioned below the microfluidic chip component 68, the centrifugal component is connected with the microfluidic chip component 68, and the reaction mixing component is positioned above the microfluidic chip component 68; the observation assembly is arranged on the outer side of the shell assembly and is positioned on the light path of the fluorescence detection assembly; the centrifugal component drives the microfluidic chip component 68 to rotate so as to centrifuge the reagent to be detected in the microfluidic chip component 68 to a corresponding position; the heating component controls the temperature in the microfluidic chip component 68 so as to amplify the reagent to be detected in the microfluidic chip component 68; the reaction mixing component intermittently attracts the magnetic beads in the microfluidic chip component 68 to move through magnetic force so as to mix the reagent to be detected in the microfluidic chip component 68 with the fluorescent marking reagent; and the fluorescence detection assembly emits detection light to the microfluidic chip assembly 68, and outputs a corresponding detection result through the observation assembly.

In this embodiment, the housing assembly includes: a main casing 16 and a casing cover 15; the shell cover 15 is hinged to the top of the main shell 16; a groove I is formed at the top of the main shell 16 and is used for loading the heating component, the centrifugal component, the fluorescence detection component and the microfluidic chip component 68; a second groove is formed in the shell cover 15 and is used for accommodating the reaction mixing component; the observation component is movably mounted on the shell cover 15, and a first window for communicating the second groove is formed in the shell cover 15.

In this embodiment, the main shell 16 and the shell cover 15 are made of black PLA engineering plastics, and 3D printing is used, so that the device has the advantages of low quality, portability, and certain heat preservation property, and meanwhile, the black material can reduce interference of the environment on visual fluorescent detection.

In this embodiment, the centrifugal assembly includes a centrifugal motor 41, the centrifugal motor 41 is fixed on the core seat main body 35 through a fixing hole IV 58, the rotating component and the shaft sleeve 44 of the centrifugal motor extend out of the surface of the core seat main body 35 through a shaft hole 59, the main control board 39 controls the output power of the centrifugal motor 41, and meanwhile, the functions of centrifugation and position control are realized by detecting the cooperation of the positioning magnet 45 and the hall sensor 33.

In this embodiment, the microfluidic chip assembly 68 includes: a microfluidic chip cover plate 11, a microfluidic chip intermediate layer 12 and a microfluidic chip bottom plate 13; the microfluidic chip cover plate 11, the microfluidic chip intermediate layer 12 and the microfluidic chip bottom plate 13 are sequentially stacked from top to bottom; the microfluidic chip cover plate 11 is provided with a sample adding hole 1, and the microfluidic chip intermediate layer 12 is provided with a detection hole; the microfluidic chip cover plate 11 and the microfluidic chip bottom plate 13 are solid at the positions corresponding to the detection holes, and the sample adding holes 1 are communicated with the detection holes.

In this embodiment, the microfluidic chip cover plate 11, the microfluidic chip intermediate layer 12, and the microfluidic chip bottom plate 13 are integrally and axisymmetrically arranged, and meanwhile, the microfluidic chip cover plate 11 and the microfluidic chip bottom plate 13 clamp the microfluidic chip intermediate layer 12 to form multiple groups of detection units with the same structure, so that rapid multi-index joint inspection for the same sample to be detected can be realized.

In this embodiment, the microfluidic chip cover plate 11, the microfluidic chip intermediate layer 12, and the microfluidic chip bottom plate 13 are all provided with bonding positioning holes 2, and the microfluidic chip cover plate 11, the microfluidic chip intermediate layer 12, and the microfluidic chip bottom plate 13 are bonded and fixed by double-sided adhesive bonding, so that the accuracy of the microfluidic chip assembly 68 can be ensured.

In this embodiment, the micro-fluidic chip cover plate 11 is provided with the air hole 3, the air hole 3 is communicated with the detection hole, and the air hole 3 is communicated with the detection hole and the external atmospheric pressure, so that the sample application is smooth, the micro-fluidic chip only needs to be injected once for a plurality of detection units, and meanwhile, only the sample application hole 1 and the air hole 3 need to be closed, so that the operation flow is simple and the use is convenient.

In this embodiment, the detection hole is divided into a plurality of sub-holes; the sub-holes comprise a quantitative hole, a first connecting hole, a buffer hole, a second connecting hole and a reaction hole; the quantitative hole, the first connecting hole, the buffer hole, the second connecting hole and the reaction hole respectively form a quantitative cavity 8, a first channel 9, a buffer cavity 7, a second channel 5 and a reaction cavity 10 together with a microfluidic chip cover plate 11 and a microfluidic chip bottom plate 13; any one of the quantifying chambers 8 is communicated with the adjacent quantifying chamber 8 through the second channel 5, and the quantifying chamber 8 is communicated with the reaction chamber 10 through the first channel 9 and the buffer chamber 7.

In this embodiment, the centrifugal motor 41, the hall sensor 33, the main control board 39, the heating position magnet 48, the detecting position magnet 45 and the microfluidic chip magnet 67 are mutually matched, so that position control can be realized, and the specific flow is as follows: the hall sensor 33 can detect the micro-fluidic chip magnet 67, after detecting the signal, the main control board 39 will adjust the rotation speed of the centrifugal motor 41 according to the signal, and the control flow is as follows: the power supply voltage of the centrifugal motor 41 is adjusted through the output of the PWM duty ratio signal of the main control board 39, so that the motor rotating speed is adjusted, the micro-fluidic chip assembly 68 slowly rotates until the micro-fluidic chip magnet 67 and the heating position magnet 48 are adsorbed, at the moment, the reaction cavities 10 of all detection units in the micro-fluidic chip assembly 68 are positioned right above all heating areas of the PCB heating circuit board 34, and at the moment, heating amplification can be started; the detection position magnet 45 is responsible for rotating the reaction cavity 10 of each detection unit by a fixed angle to the detection position for fluorescence detection.

Specifically, the microfluidic chip cover plate 11, the microfluidic chip intermediate layer 12, and the microfluidic chip bottom plate 13 are integrally disc-shaped structures, and preferably: radius 26mm, height 4mm; each detection unit is positioned in the radial direction of the circular structure.

In this embodiment, the supporting columns 57 on the surface of the core seat main body 35 are responsible for fixing the PCB heating circuit board 34, and lifting the PCB heating circuit board 34 so as not to contact with the surface of the core seat main body 35, thereby reducing heat transfer; meanwhile, the heat insulation grooves 4 are arranged on two sides of each detection unit reaction cavity 10 in the three-layer structure in the microfluidic chip assembly 68, so that heat dissipation around the reaction cavities 10 can be effectively insulated, and heating efficiency is improved. The structure of the PCB heating circuit board 34 is an annular structure, and a notch (the angle is about 47 degrees) is designed, so that the angle is reserved for visual fluorescence detection, and the visual fluorescence detection is started by matching with the rotation of different reaction cavities 10 in the microfluidic chip assembly 68 to the detection position.

In this embodiment, the reaction mixing element comprises: a mixing driving member 32, a fixing frame 30, a plurality of mixing magnets 31 and a plurality of magnetic beads; the mixing driving piece 32 is positioned in the second groove, and the mixing driving piece 32 is connected with the shell cover 15; the fixing frame 30 is connected with a mixed driving piece 32, and each mixed magnet 31 is arranged on the fixing frame 30; each magnetic bead is respectively positioned in the corresponding reaction cavity 10.

In this embodiment, the hybrid drive 32 employs a small DC coreless motor.

Specifically, the rotation speed of the mixing driving element 32 is adjusted by setting the PWM output duty ratio of the main control board 39, so as to determine a more appropriate rotation speed, and realize the mixing of the magnetic beads in the reaction cavity 10 in the microfluidic chip assembly 68.

In this embodiment, after the cover 15 is closed, the reaction mixing assembly is parallel to the microfluidic chip assembly 68, the mixing magnet 31 is located directly above the reaction chamber 10 and is not in contact with the microfluidic chip cover 11, the distance between the mixing magnet 31 and the microfluidic chip cover 11 is 0.5mm, and when the mixing driving member 32 rotates, the magnetic beads inside the reaction chamber 10 can be mixed under the action of magnetic attraction.

In this embodiment, the fluorescence detection assembly includes: an LED light-emitting substrate 43, a light-homogenizing plate 40, a first filter 27 and a second filter 29; the LED light-emitting substrate 43, the light-homogenizing plate 40 and the first filter 27 are sequentially arranged below the microfluidic chip assembly 68 from bottom to top, the second filter 29 is installed in the first window, and the microfluidic chip assembly 68 is located between the first filter 27 and the second filter 29.

In this embodiment, the observation assembly includes: a magnifying glass 23; the magnifier 23 is mounted on the housing assembly, and the viewing direction of the magnifier 23 is set toward the second filter 29.

In this embodiment, the magnifier 23 is movably connected to the housing assembly via a bracket 24 and a damping shaft 28.

In this embodiment, a transparent PMMA protective shell 22 is also mounted in window one: the LED light-emitting substrate 43, the light-homogenizing plate 40, the first optical filter 27, the second optical filter 29, the transparent PMMA protective shell 22 and the magnifier 23 form a vertical light path; the specific light path is: the LED light-emitting substrate 43 is excitation light, and the central wavelength is 452nm; then vertically enters the light homogenizing plate 40 to change the LED point light source into a surface light source, and the wavelength (type) of the light is unchanged; then vertically enters the first filter 27 to excite the FAM fluorescent group (5-Carboxyfluorescein, which is a common fluorescent labeling reagent), and the FAM fluorescent group emits green fluorescence when excited by blue light; the light then enters the second filter 29; finally, the light enters a transparent PMMA protective shell 22 and a magnifier 23, the transparent PMMA protective shell 22 plays a role of protecting a second optical filter 29, the magnifier 23 plays a role of magnifying an observation area, and the transparent PMMA protective shell and the magnifier have no influence on the wavelength (type) of the light; the relative positions of the light path structures are as follows: the LED light-emitting substrate 43 is positioned at the bottommost part of the light path structure, the light-homogenizing plate 40 is positioned at the position about 12mm above the LED light-emitting substrate 43, the first optical filter 27 is positioned at the position about 9mm above the light-homogenizing plate 40, the first optical filter 27 and the light-homogenizing plate are in close contact with each other, the reaction cavity 10 is positioned at the position about 4mm above the first optical filter 27, the second optical filter 29 is positioned at the position about 11.5mm above the reaction cavity 10, the transparent PMMA protective shell 22 is positioned at the position about 29 mm above the second optical filter 29, the first optical filter and the second optical filter are in close contact with each other, and the magnifying glass 23 is positioned at the position about 9mm above the transparent PMMA protective shell 22; the core mount body 35, core mount bottom plate 36, bracket 24, and damping shaft 28 provide fixation assistance for the optical structure, and all optical structure components are parallel to each other when performing visual fluorescence detection.

In this embodiment, the LED light emitting substrate 43 is fixed on the core base plate 36 by screws, the light homogenizing plate 40 and the first optical filter 27 are vertically stacked in the mounting groove 55, the second optical filter 29 is located inside the housing cover 15, and the transparent PMMA protective shell 22 with a thickness of 1mm is located outside the housing cover 15, so as to physically protect the second optical filter 29. The second filter 29 and the transparent PMMA shield 22 are isosceles trapezoids in shape, maximizing the viewing area.

In this embodiment, the power supply and control module includes power supply module and main control circuit board, realizes stable power supply and signal acquisition and control function, and button indicating module includes two buttons, 1 bicolor pilot lamp and 1 monochromatic lamp, realizes button control and signal display.

Referring to fig. 1, 2, 9 and 10, a bottom mounting plate 17, a battery compartment housing 18 and a battery compartment bottom plate 19 are sequentially arranged at the bottom of the main housing 16; the shell cover 15 is fixedly connected with the main shell 16 through a rotating hinge 25, a fixed hinge 26 and screws, the rotating hinge 25 is fixed on the shell cover 15 through screws, and the fixed hinge 26 is fixed on the shell cover 15 through screws, so that the shell cover 15 is ensured to be opened and closed; the main shell 16 and the bottom mounting plate 17 are connected through screws, so that the fastening of the shell assembly is ensured; the battery compartment shell 18 and the battery compartment bottom plate 19 are fixed by using screws, so that the fastening of the battery compartment is ensured. The main housing 16 has a key indicating circuit board 38, a power switch 20 and a Type-C power interface 21. The Type-C power interface 21 is matched with a power adapter for use, the power of the power adapter is 10W (5V/2A), meanwhile, 18650 lithium batteries (3.7V/3000 mAh) can be used for supplying power, the functions of dual power switching and lithium battery charging can be realized, stable power output is provided for the device, and the device can be ensured to work normally and stably. The lithium battery module 52 is disposed within the battery compartment housing 18 and the battery compartment floor 19. The bottom mounting plate 17 and the battery compartment housing 18 are fixedly adsorbed through the bottom plate magnet 61 positioned on the outer side of the bottom mounting plate 17 and the battery compartment magnet 66 positioned on the outer side of the battery compartment housing 18, and the battery compartment housing is selectively fixedly adsorbed when in use, and can be directly removed without complex operation when not in use, and the overall size of the device is 72 x 65 x 95mm (the size of the detachable battery compartment is 72 x 65 x 27 mm). The cover 15 is provided with a cylindrical closing magnet II 51, the main shell 16 is provided with a cylindrical closing magnet I49, and when the cover 15 is closed, the closing stability of the device is ensured through the magnetic attraction effect. The transparent PMMA protective shell 22 is positioned on the surface of the shell cover 15 and is used for protecting the second optical filter 29; the magnifying glass 23 is fixed by the support 24, the support 24 is fixed on the shell cover 15 by the damping rotating shaft 28, and the support 24 can rotate around the damping rotating shaft 28, so that whether the magnifying glass 23 is used or not can be selected during observation (the selection basis is that the observation area is magnified during observation by using the magnifying glass 23, and the observation effect is optimized). However, since the magnifying glass 23 needs to adjust the distance between the naked eye and the magnifying glass 23 during observation, so as to realize focal length matching, the magnifying glass 23 can be omitted, and direct naked eye observation can be performed, and when the magnifying glass 23 is used for observation, the bracket 24 is rotated to be right above the second optical filter 29, so that the observation area can be enlarged, and the visual observation effect can be optimized. The damping rotating shaft 28 is 4mm in diameter, 19mm in height and 0.1N.m in torsion, so that the damping rotating shaft can be fixed at any angle by one hand, the magnifying glass 23 adopts an 18-multiplying power magnifying glass 23, and the damping rotating shaft is of the following size: diameter 25mm and thickness 4mm. Referring to fig. 3, the microfluidic chip magnets 67 (with a size of 2mm and a height of 4 mm) are symmetrically distributed on two sides of the microfluidic chip assembly 68 along the diameter thereof, and the purpose of designing two microfluidic chip magnets 67 is to adsorb the heating position magnet 48 (with a size of 3mm and a height of 3 mm) and the detecting position magnet 45 (with a size of 3mm and a height of 3 mm), and at the same time, the symmetrical design is beneficial to keeping balance when the microfluidic chip assembly 68 rotates at a high speed and guaranteeing moment balance when the magnets are adsorbed. Referring to fig. 4, 5 and 6, the cover plate 11 and the bottom plate 13 are transparent PMMA with a thickness of 1mm, the middle layer 12 of the microfluidic chip is made of PMMA with a thickness of 2mm, and the frosted material can effectively reduce scattering of incident light compared with the PMMA with a mirror surface material, so that interference can be reduced. The mirror surface of the intermediate layer 12 of the micro-fluidic chip is combined with the cover plate 11 of the micro-fluidic chip, and the frosted surface of the intermediate layer 12 of the micro-fluidic chip is combined with the bottom plate 13 of the micro-fluidic chip. Specifically, the reagent to be measured is injected from the sample injection hole 1 into the second channel 5 sequentially passing through each detection unit, and fills the quantitative cavity 8 of each detection unit. The depth of the first channel 9 and the buffer cavity 7 of the micro-fluidic chip interlayer 12 is 0.4mm, the width of the first channel 9 is 0.24mm, the radius of the buffer cavity 7 is 0.3mm, and the magnetic beads and the dry powder which are pre-placed in the reaction cavity 10 are prevented from entering other cavities. Compared with the second channel 5 and the quantifying cavity 8, the first channel 9 and the buffer cavity 7 are micro channels (the width of the second channel 5 is 0.45mm, the depth is 2mm, the depth of the quantifying cavity 8 is 2mm, the volumes of the second channel 5 and the quantifying cavity 8 of each group of detection units are about 25 ul), the fluid passing resistance is large, the reagent or the sample can not break through into the reaction cavity 10 during sample injection, the quantifying cavity 8 and the second channel 5 are filled preferentially, and reagent quantification of each detection unit is realized. And because of the first channel 9 and the micro channel of the buffer cavity 7, the loss of the magnetic beads and the dry powder placed in the reaction cavity 10 is avoided (the depth of the reaction cavity 10 is 2mm, the volume is about 25.8ul, the volume of the magnetic beads is about 0.5ul, and the volume of the dry powder sphere is about 0.3 ul). After the reagent injection is completed, the sample addition well 1 and the air hole 3 are closed. The microfluidic chip assembly 68 is placed on the shaft sleeve 44 tightly matched above the centrifugal motor 41, the centrifugal motor 41 starts to centrifuge, the microfluidic chip assembly 68 moves circularly around the motor shaft at a high speed, at this time, reagents in the quantifying cavity 8 and the second channel 5 of each detecting unit are replaced by gas and liquid through the first channel 9 and the buffer cavity 7 so as to enter the reaction cavity 10, at this time, the reaction cavity 10 is completely filled with the reagents, and at the same time, the microfluidic chip assembly further comprises magnetic beads, at this time, the dry powder is dissolved. After the reagent enters the reaction chambers 10, the quantifying chambers 8 and the second channels 5 of the microfluidic chip become cavities, and air isolation between the reaction chambers 10 is realized during heating amplification. The centrifugal motor 41 is a small-sized direct-current hollow cup motor, the diameter is 15 mm, the height is 17: 17 mm (including a motor shaft), the power supply voltage is 3.3V, and the control of the power supply voltage of the centrifugal motor 41 can be realized by controlling the output of a PWM duty ratio signal of the main control board 39, so that the regulation of the motor rotating speed is realized. Under the working condition that the device is in a centrifugal mode, the PWM duty ratio is adjusted to be 100%, the voltage at the two ends of the centrifugal motor 41 is 3.3V, and the rotating speed of the centrifugal motor 41 is the highest rotating speed, so that high-speed centrifugal action is realized. The reagent injection, reagent quantitative distribution of the four detection channels and centrifugation are completed, the sample injection and the sealing of the air hole 3 are only required to be carried out once, and the follow-up work is an automatic device flow. The first fixing hole 6 and the shaft sleeve 44 in the microfluidic chip assembly 68 are in a circular-segment structure, and the angle matching of the first fixing hole 6 and the shaft sleeve 44 also ensures that the microfluidic chip assembly 68 cannot move relatively during high-speed rotation. The micro-fluidic chip bottom plate 13 is a simple bearing structure and is provided with a heat insulation groove 4, a first positioning hole 14, a bonding positioning hole 2 and a first fixing hole 6. The support column 57 on the surface of the core base main body 35 is responsible for fixing the PCB heating circuit board 34, the front surface (with component layers) of the PCB heating circuit board 34 faces the surface of the core base main body 35, and the back surface (the plane on the windowed side of the circuit board) of the PCB heating circuit board 34 is used for heating and faces the microfluidic chip assembly 68. The support column 57 lifts the PCB heating circuit board 34 without contacting the surface of the core holder main body 35, reducing heat transfer, and improving heating efficiency. The wiring harness of the PCB heating circuit board 34 will be accessed to the main control board 39 through the device wiring harness aperture three 56. The microfluidic chip assembly 68 performs the heating position control under the cooperation of the centrifugal motor 41, the hall sensor 33, the heating position magnet 48, and the microfluidic chip magnet 67 after the reagent injection, the reagent quantitative distribution, and the centrifugation are completed. Any one of the magnets 67 of the microfluidic chip is attracted to the magnet 48 of the heating position, and at this time, the microfluidic chip assembly 68 is located at the heating position, and four heating areas of the PCB heating circuit board 34 are located under the reaction chambers 10 of the four detection units. The centrifugal motor 41 specifically performs the following steps: the main control board 39 outputs a PWM duty ratio of 10% at this time, and reduces the rotation speed of the centrifugal motor 41, at this time, the microfluidic chip assembly 68 starts to rotate at a low speed, and when the microfluidic chip magnet 67 rotates past the hall sensor 33, the main control board 39 will detect the signal, then adjust the PWM duty ratio to 0%, at this time, the centrifugal motor 41 stops rotating, and at the same time, since the microfluidic chip assembly 68 has a certain inertia and the heating position magnet 48 is fixed on the main housing 16, this will adsorb the microfluidic chip magnet 67, and finally realize that the microfluidic chip assembly 68 rotates to a fixed angle, so that it stops at the heating position. At this point, the reaction chambers 10 of the detection units in the microfluidic chip assembly 68 are located directly above the rectangular heating areas of the PCB heating circuit board 34, at which time non-contact thermal amplification may begin. Referring to fig. 9, 10 and 13, after the microfluidic chip assembly 68 is placed, the relative positions of the core holder body 35, the hybrid driving member 32, the magnetic mount 30 and the hall sensor 33 are determined. The microfluidic chip assembly 68 is located above the PCB heating circuit board 34 with an interval of 0.8mm, and non-contact heating is adopted, so that the heating and rotation of the microfluidic chip assembly 68 are not interfered with each other, and the structural design is simplified. (non-contact heating primarily transfers energy to the target object by means of electromagnetic radiation, transfers to the object surface and converts it into heat, causing it to heat up). The mixing driver 32 is fixed to the cover 15 by screws, a fixing frame 30 is fixed to the mixing driver 32, and mixing magnets 31 (the size is 3mm in diameter and 3mm in height) are fixed to both ends of the fixing frame 30. The mixing magnet 31 is fixed at both ends, so that the mixing efficiency of the magnetic beads is improved and the fixing frame 30 is balanced while rotating. After the shell cover 15 is closed, the mixed driving piece 32 is located right above the microfluidic chip assembly 68, and when the mixed driving piece 32 rotates, the mixed magnet 31 at the two ends and the fixing piece are driven to perform circular motion around a motor shaft, and the motion track of the mixed magnet 31 can cover the reaction cavity 10 of each detection unit of the microfluidic chip assembly 68, so that magnetic beads pre-buried in the reaction cavity 10 can be driven to mix, and the amplification reaction efficiency is improved. The magnetic bead mixing process can be carried out every 2 minutes during the heating amplification. The hybrid driving part 32 is a small direct current hollow cup motor, the diameter is 6mm, the height is 16.5mm (including a motor shaft), the power supply voltage is 3.3V, and the control of the power supply voltage of the hybrid driving part 32 can be realized by controlling the output of a PWM duty ratio signal of the main control board 3939, so that the regulation of the motor rotating speed is realized. Since the main function of the motor is to mix magnetic beads, the rotating speed is not easy to be too fast, and tests show that when the PWM duty ratio is adjusted to be 20%, the adsorption effect of the mixed magnet 31 on the magnetic beads in the microfluidic chip assembly 68 is good. Therefore, the PWM duty ratio outputted from the main control board 39 is 20%, and the rotation speed of the hybrid driving member 32 is suitable. Meanwhile, the mixing magnet 31 and the microfluidic chip assembly 68 are in non-contact, so that the mixing function of magnetic beads in the microfluidic chip assembly 68 is realized, the circular motion of the microfluidic chip assembly 68 is not influenced, the heat dissipation of the microfluidic chip assembly 68 to the outside during amplification is reduced, and the interval between the mixing magnet 31 and the microfluidic chip assembly 68 is 0.5mm. The centrifugal motor 41 and the LED light-emitting substrate 43 are located between the core base body 35 and the core base bottom plate 36, wherein the centrifugal motor 41 is fixed on the core base body 35 through the fixing hole four 58 by using screws, and the LED light-emitting substrate 43 is fixed on the core base bottom plate 36 by using screws. The rotating part of the centrifugal motor 41 and the shaft sleeve 44 extend out of the surface of the core holder main body 35 through the shaft hole 59. The core holder main body 35 and the core holder bottom plate 36 realize multi-part fixation, and the non-contact heating assembly, the centrifugal motor 41 and the excitation light part of the fluorescence detection assembly are fixedly placed through proper structural design at the same time by using a small space. The wire harnesses of the PCB heating circuit board 34, the centrifugal motor 41 and the LED light emitting substrate 43 are connected into the main control board 39 through the first wire harness hole 42, and the wire harness management is finished so as to be convenient for modularized installation. The wiring harness of the PCB heating circuit board 34 will be accessed to the main control board 39 through the wiring harness aperture three 56. Referring to fig. 14 and 15, the second filter 29 is located inside the cover 15, and the transparent PMMA shell 22 with a thickness of 1mm is located outside the cover 15, so as to protect the second filter 29. The second filter 29 adopts a long-wave pass filter, the cut-off wavelength is 506nm, the cut-off depth is OD3, the main function of the filter is to keep the excitation light of the FAM fluorescent group, filter the stray light of the blue wave band, and the filter is favorable for directly observing the MIRA amplification result adopting the FAM fluorescent group, adopts the superposition mode of two filters, optimizes the cut-off effect, and the size of a single filter is as follows: an isosceles trapezoid with an upper bottom of 7mm, a lower bottom of 28mm and a height of 23mm, and the thickness of the isosceles trapezoid is 1.1mm. The second optical filter 29 and the transparent PMMA protective shell 22 are isosceles trapezoids, so that the observation area is increased to the greatest extent, and visual observation is facilitated. Referring to fig. 16 and 17, the second fixing hole 50 is responsible for fixing the damping shaft 28, and can provide the magnifying glass 23 to observe the amplification result of the MIRA. The first mounting hole 47 is responsible for fixing the Hall sensor 33; the second mounting hole 53 is responsible for fixing the hybrid driving member 32 on the housing cover 15 and managing the wire harness, and the wire harness of the hybrid driving member 32 is connected to the main control board 39 through the second wire harness hole 46. Any one of the microfluidic chip magnets 67 is adsorbed corresponding to the detection position magnet 45, and at this time, the microfluidic chip assembly 68 is located at the detection position, and the filter one 27 is located under the reaction chamber 10 of one of the four detection units. The device has four detection position magnets 45 which are symmetrically distributed and have an included angle of 90 degrees. When the microfluidic chip assembly 68 is located at the detection position, it is further required to rotate 90 degrees to enable the reaction chamber 10 of the next detection unit to enter the detection position, and the above steps are repeated until all the reaction chambers 10 of the detection units are detected. When fluorescence detection is started, the LED light-emitting substrate 43 is started, meanwhile, the rotating speed of the centrifugal motor 41 is regulated, and the microfluidic chip assembly 68 can rotate by a fixed angle by matching with the microfluidic chip magnet 67 and the detection position magnet 45, so that each reaction cavity 10 can be rotated to the position right above the first optical filter 27, and at the moment, the LED light-emitting substrate 43, the light-homogenizing plate 40, the first optical filter 27, the reaction cavity 101, the second optical filter 29 and the magnifier 23 form a vertical light path structure, and visual observation can be started. The microfluidic chip assembly 68 has four groups of detection units, and the included angle between two adjacent reaction chambers 10 is 90 degrees, so that the centrifugal motor 41 can complete the detection of all the detection units by controlling the microfluidic chip assembly 68 to rotate by a fixed angle. The centrifugal motor 41 specifically performs the following steps: the micro-fluidic chip assembly 68 starts from the heating position, the PWM duty cycle outputted by the main control board 39 is 74% for 30ms, the effect is to provide a larger torque to separate from the adsorption of the heating position magnet 48, the subsequent output PWM duty cycle is reduced to 4% for 100ms, the centrifugal motor 41 is made to rotate slowly, the deceleration process is performed, the subsequent output PWM duty cycle is reduced to 0%, and the detection position magnet 45 is adsorbed by inertia. The micro-fluidic chip assembly 68 has four detection units, and can perform detection position rotation according to specific requirements, or can automatically perform rotation of the four detection units, so as to facilitate observation of rotation time intervals of 10s of each detection unit. Four holes of the fixing hole III 54 are in symmetrical structure and are responsible for being matched with the core base plate 36, and the fixing hole III is fixed by using screws. The hall sensor 33 is mounted through the inside of the main casing 16, and is fitted into the first mounting hole 47. The main control board 39 is screwed to the bottom mounting plate 17, and the power switch 20 and the key indication circuit board 38 are screwed to the main casing 16. The power supply module and the dual-power switching module are responsible for integral power supply of the device, the device can be powered by a Type-c power adapter (5V/2A) or 18650 lithium batteries (3.7V/3000 mAh), the device can realize dual-power switching of the power adapter and the lithium batteries and battery charging, the power adapter and the lithium batteries can realize independent power supply, when the power adapter and the lithium batteries are simultaneously accessed, the power adapter and the lithium batteries can charge the lithium batteries, and the Bluetooth communication module is responsible for data communication with upper computer software; the key indication circuit board 38 is responsible for man-machine interaction of device hardware, realizes key detection and related indication LED display, and has two LED indication lamps, namely a red single-color LED indication lamp 63, a red and blue double-color LED indication lamp 62, wherein the single-color lamp is used as a power indication lamp of the device, the red in the double-color lamp is used as a heating amplification indication lamp of the device, the blue is used as a fluorescence detection flow indication lamp, and has two key caps 64, namely a heating amplification key 37 and a fluorescence detection key 65 respectively, and has the functions of starting and stopping heating amplification and has the function of starting and stopping a fluorescence detection flow; the position detection module is responsible for acquiring signals obtained by detection of the Hall sensor 33; the buzzer alarm module is responsible for giving an alarm to prompt a user of the device after the heating amplification is finished, and the power switch 20 is responsible for turning on and off the total power supply of the device. The fourth wire bundle hole 60 is responsible for connecting the main control board 39 with the wire bundle of the lithium battery module 52, and the bottom plate magnet 61 is arranged outside the bottom mounting plate 17. The relative positions of the battery compartment housing 18, the battery compartment floor 19, and the lithium battery module 52, the lithium battery module 52 is disposed within the battery compartment housing 18 and the battery compartment floor 19. The battery compartment magnet 66 is mounted on the outer side of the battery compartment housing 18, and can be attracted to the bottom plate magnet 61 of the bottom mounting plate 17, so that the battery compartment housing can be attracted and fixed when a lithium battery is selected for use, and the battery compartment housing can be directly removed without complex operation when not used. The PCB heating circuit board 34 integrates a temperature measuring chip 70 and a heating resistor 69, and shares a four-channel heating area. The power supply voltage is 5V, the total heating power is 6.1W, the resistance value of a single heating resistor 69 is 33 ohms, the components are packaged as 2010, and the rated power is 1.5W. The four heating areas are all arranged on the PCB heating circuit board 34, the included angle of each heating area is 90 degrees, the size is about 10 x 9mm, the front (component side) of the PCB heating circuit board 34 faces downwards, the bottom layer faces upwards (the side facing the micro-fluidic chip bottom plate 13) during installation, non-contact heating is realized, the micro-fluidic chip assembly 68 is arranged above the PCB heating circuit board 34, and the interval is 0.8mm. On the front surface of the PCB heating circuit board 34, each heating area is composed of 2 heating resistors 69 and a temperature measuring chip 70, the distribution is that the heating resistors 69 are symmetrically and uniformly distributed in the heating area, the temperature measuring chip 70 is located between the two heating resistors 69, the symmetrical design layout of the heating resistors 69 ensures the uniformity of the heating area, the temperature measuring chip 70 is located at the center of the heating area, and the temperature measuring precision is ensured. The area of each heating area of the PCB heating circuit is larger than the area of the reaction cavity 10 of the microfluidic chip (the size of each reaction cavity 10 is about 5.1 mm by 2.8 mm), the area of the reaction cavity 10 can be covered, the heating amplification efficiency and stability are guaranteed, meanwhile, the size of the PCB heating circuit board 34 at the rest part is reduced as much as possible (comprising the joints of different heating areas, the binding posts 71 and the fixing holes five 72), the purpose is to reduce heat dissipation, and the heating efficiency of the heating area is improved. The PCB heating circuit board 34 is of a ring structure, but is designed with a notch (with an angle of about 47 degrees) for setting an angle for the visual fluorescence detection light path, and a visual fluorescence detection optical module will be provided in the vertical direction at the notch, and the notch can cooperate with different reaction chambers 10 in the microfluidic chip assembly 68 to rotate to the fluorescence detection position to start visual fluorescence detection.

Embodiment 2, on the basis of embodiment 1, the present embodiment provides a detection method using the multi-target detection device suitable for a microfluidic chip as provided in embodiment 1, which includes: the micro-fluidic chip assembly 68 is driven to rotate through the centrifugal assembly, so that the reagent to be detected in the micro-fluidic chip assembly 68 is centrifuged to a corresponding position; controlling the temperature in the microfluidic chip assembly 68 by the heating assembly so as to amplify the reagent to be detected in the microfluidic chip assembly 68; intermittently attracting the magnetic beads in the microfluidic chip assembly 68 to move by magnetic force through the reaction mixing assembly so as to mix the reagent to be detected in the microfluidic chip assembly 68 with the fluorescent labeling reagent; the detection light is emitted to the microfluidic chip assembly 68 by the fluorescence detection assembly, and the corresponding detection result is output via the observation assembly.

In summary, the heating component and the microfluidic chip component are arranged in a non-contact manner, so that the centrifugal, heating and amplification of the reagent to be detected can be realized quickly, the reaction mixing component can uniformly mix the reagent to be detected and the fluorescent marking reagent, and meanwhile, the fluorescent detection component and the observation component are matched to realize the automation of the quick multi-target detection and detection process, and the centrifugal, heating and amplification device has the advantages of small volume, low cost, simple structure, convenience in operation and easiness in carrying; the visual fluorescence detection method is used for completing the detection of a plurality of detection units in the centrifugal microfluidic chip, completing the detection of a plurality of targets, and simultaneously realizing the automation of the device operation by the opto-electromechanical integration of the instrument device so as to be suitable for POCT.

The components (components not illustrating the specific structure) selected in the present application are common standard components or components known to those skilled in the art, and the structures and principles thereof are known to those skilled in the art through technical manuals or through routine experimental methods.

In the description of embodiments of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

With the above-described preferred embodiments according to the present invention as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but must be determined according to the scope of claims.

Claims (10)

1. A multi-target detection device suitable for use in a microfluidic chip, comprising:

The device comprises a shell assembly, a heating assembly, a centrifugal assembly, a fluorescence detection assembly, a microfluidic chip assembly, a reaction mixing assembly and an observation assembly; wherein the method comprises the steps of

The heating component, the centrifugal component, the fluorescence detection component, the microfluidic chip component and the reaction mixing component are arranged in the shell component, the heating component, the centrifugal component and the fluorescence detection component are positioned below the microfluidic chip component, the centrifugal component is connected with the microfluidic chip component, and the reaction mixing component is positioned above the microfluidic chip component;

the observation assembly is arranged on the outer side of the shell assembly and is positioned on the light path of the fluorescence detection assembly;

the centrifugal component drives the microfluidic chip component to rotate so as to centrifuge the reagent to be detected in the microfluidic chip component to a corresponding position;

The heating component controls the temperature in the microfluidic chip component so as to amplify the reagent to be detected in the microfluidic chip component;

The reaction mixing assembly intermittently attracts magnetic beads in the microfluidic chip assembly to move through magnetic force so as to mix a reagent to be detected in the microfluidic chip assembly with a fluorescent marking reagent; and

The fluorescence detection component sends out detection light to the microfluidic chip component, and the corresponding detection result is output through the observation component.

2. The multi-target detection device for a microfluidic chip according to claim 1,

The housing assembly includes: a main housing and a housing cover;

the shell cover is hinged to the top of the main shell;

A groove I is formed in the top of the main shell and is used for accommodating the heating component, the centrifugal component, the fluorescence detection component and the microfluidic chip component;

A second groove is formed in the shell cover and used for accommodating the reaction mixing component;

the observation assembly is movably arranged on the shell cover, and a first window communicated with the second groove is formed in the shell cover.

3. The multi-target detection device for a microfluidic chip according to claim 2,

The microfluidic chip assembly includes: a microfluidic chip cover plate, a microfluidic chip intermediate layer and a microfluidic chip bottom plate;

the microfluidic chip cover plate, the microfluidic chip intermediate layer and the microfluidic chip bottom plate are sequentially stacked from top to bottom;

a sample adding hole is formed in the microfluidic chip cover plate, and a detection hole is formed in the middle layer of the microfluidic chip;

The microfluidic chip cover plate and the microfluidic chip bottom plate are solid and are communicated with the detection holes at the positions corresponding to the detection holes.

4. The multi-target detection device for a microfluidic chip according to claim 3,

And the microfluidic chip cover plate is provided with air holes which are communicated with the detection holes.

5. The multi-target detection device for a microfluidic chip according to claim 3,

The detection hole is divided into a plurality of sub-holes;

The sub-holes comprise a quantitative hole, a first connecting hole, a buffer hole, a second connecting hole and a reaction hole;

The quantitative hole, the first connecting hole, the buffer hole, the second connecting hole and the reaction hole respectively form a quantitative cavity, a first channel, a buffer cavity, a second channel and a reaction cavity with the microfluidic chip cover plate and the microfluidic chip bottom plate;

Any one of the quantifying cavities is communicated with the adjacent quantifying cavity through the second channel, and the quantifying cavity is communicated with the reaction cavity through the first channel and the buffer cavity.

6. The multi-target detection device for a microfluidic chip according to claim 5,

The reaction mixing element comprises: the device comprises a mixed driving piece, a fixed frame, a plurality of mixed magnets and a plurality of magnetic beads;

The mixing driving piece is positioned in the second groove and is connected with the shell cover;

The fixing frame is connected with the mixed driving piece, and each mixed magnet is arranged on the fixing frame;

each magnetic bead is respectively positioned in the corresponding reaction cavity.

7. The multi-target detection device for a microfluidic chip according to claim 2,

The fluorescence detection assembly includes: the LED light emitting substrate, the light homogenizing plate, the first optical filter and the second optical filter;

The LED light emitting substrate, the light homogenizing plate and the first optical filter are sequentially arranged below the micro-fluidic chip assembly from bottom to top, the second optical filter is arranged in the first window, and the micro-fluidic chip assembly is positioned between the first optical filter and the second optical filter.

8. The multi-target detection device for a microfluidic chip according to claim 7,

The viewing assembly includes: a magnifying glass;

the magnifying glass is arranged on the shell component, and the observation direction of the magnifying glass faces to the second optical filter.

9. The multi-target detection device for a microfluidic chip according to claim 8,

The magnifier is movably connected with the shell assembly through the bracket and the damping rotating shaft.

10. A detection method using the multi-target detection device for a microfluidic chip according to any one of claims 1 to 9, comprising:

Driving the micro-fluidic chip assembly to rotate through the centrifugal assembly so as to centrifuge the reagent to be detected in the micro-fluidic chip assembly to a corresponding position;

Controlling the temperature in the microfluidic chip assembly through the heating assembly so as to amplify the reagent to be detected in the microfluidic chip assembly;

intermittently attracting magnetic beads in the microfluidic chip assembly to move through magnetic force by the reaction mixing assembly so as to mix the reagent to be detected in the microfluidic chip assembly with the fluorescent marking reagent;

And detecting light rays are sent out to the microfluidic chip assembly through the fluorescence detection assembly, and corresponding detection results are output through the observation assembly.