CN118076731A - 涉及逆转座子和其功能片段的系统、组合物和方法 - Google Patents

涉及逆转座子和其功能片段的系统、组合物和方法 Download PDF

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Publication number
CN118076731A
CN118076731A CN202280062406.5A CN202280062406A CN118076731A CN 118076731 A CN118076731 A CN 118076731A CN 202280062406 A CN202280062406 A CN 202280062406A CN 118076731 A CN118076731 A CN 118076731A
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sequence
reverse transcriptase
seq
domain
nos
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Chinese (zh)
Inventor
布莱恩·C·托马斯
克利斯多佛·布朗
丹妮拉·S·A·戈尔茨曼
莎拉·拉佩里埃
辛迪·卡斯泰勒
利萨·亚历山大
玛丽·凯特琳·赵
莫拉伊玛·特莫彻-迪亚兹
安努·托马斯
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Macrogenomics
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Macrogenomics
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Publication of CN118076731A publication Critical patent/CN118076731A/zh
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/18011—Details ssRNA Bacteriophages positive-sense
    • C12N2795/18111—Leviviridae
    • C12N2795/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2800/101—Plasmid DNA for bacteria
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

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  • Bioinformatics & Cheminformatics (AREA)
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  • Physics & Mathematics (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202280062406.5A 2021-09-08 2022-09-07 涉及逆转座子和其功能片段的系统、组合物和方法 Pending CN118076731A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163241943P 2021-09-08 2021-09-08
US63/241,943 2021-09-08
PCT/US2022/076061 WO2023039438A1 (en) 2021-09-08 2022-09-07 Systems, compositions, and methods involving retrotransposons and functional fragments thereof

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CN118076731A true CN118076731A (zh) 2024-05-24

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CN202280062406.5A Pending CN118076731A (zh) 2021-09-08 2022-09-07 涉及逆转座子和其功能片段的系统、组合物和方法

Country Status (9)

Country Link
US (1) US20240287484A1 (https=)
EP (1) EP4399299A4 (https=)
JP (1) JP2024534856A (https=)
KR (1) KR20240051994A (https=)
CN (1) CN118076731A (https=)
AU (1) AU2022343719A1 (https=)
CA (1) CA3230213A1 (https=)
MX (1) MX2024003006A (https=)
WO (1) WO2023039438A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024212753A1 (en) * 2023-04-11 2024-10-17 Beijing Astragenomics Technology Co., Ltd. Non-ltr retrotransposon system and use thereof
WO2025199534A1 (en) * 2024-03-22 2025-09-25 Typewriter Therapeutics, Inc. New r2 retrotransposons for gene writing

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3844272A1 (en) * 2018-08-28 2021-07-07 Flagship Pioneering Innovations VI, LLC Methods and compositions for modulating a genome
US20220298495A1 (en) * 2019-06-12 2022-09-22 Emendobio Inc. Novel genome editing tool
CN114981409A (zh) * 2019-09-03 2022-08-30 美洛德生物医药公司 用于基因组整合的方法和组合物

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CA3230213A1 (en) 2023-03-16
EP4399299A1 (en) 2024-07-17
EP4399299A4 (en) 2025-09-03
WO2023039438A1 (en) 2023-03-16
MX2024003006A (es) 2024-03-25
JP2024534856A (ja) 2024-09-26
US20240287484A1 (en) 2024-08-29
KR20240051994A (ko) 2024-04-22
AU2022343719A1 (en) 2024-04-11

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