CN117987245A - High-efficient extraction element of whole genome of rice - Google Patents

High-efficient extraction element of whole genome of rice Download PDF

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Publication number
CN117987245A
CN117987245A CN202410396610.1A CN202410396610A CN117987245A CN 117987245 A CN117987245 A CN 117987245A CN 202410396610 A CN202410396610 A CN 202410396610A CN 117987245 A CN117987245 A CN 117987245A
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China
Prior art keywords
container
centrifugal
driving
workbench
piston
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CN202410396610.1A
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Chinese (zh)
Inventor
陆文静
周通
王桂玲
刘乃生
张云江
马文东
王立楠
王曼力
蔡丽君
孙秋霞
王阔
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Rice Research Institute Of Heilongjiang Academy Of Agricultural Sciences
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Rice Research Institute Of Heilongjiang Academy Of Agricultural Sciences
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Priority to CN202410396610.1A priority Critical patent/CN117987245A/en
Publication of CN117987245A publication Critical patent/CN117987245A/en
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Abstract

The invention relates to the technical field of biological equipment, in particular to a rice whole genome efficient extraction device, which comprises: the device comprises a main body and a driving mechanism, wherein the main body comprises a workbench and a fixed container, the workbench provides an installation space for other components, the fixed container is installed on the workbench, the driving mechanism comprises a centrifugal driving motor, a cross driving shaft, a transmission sleeve and a piston, the centrifugal driving motor is installed at the top of the workbench, an output shaft of the centrifugal driving motor is fixedly connected with the cross driving shaft through a bolt, the transmission sleeve is sleeved on the outer side of the cross driving shaft, and the bottom end of the transmission sleeve is fixedly connected with the piston; the device ensures that the rice cell membrane is fully destroyed by combining rotation centrifugation and pressurization, thereby being capable of rapidly and efficiently extracting rice cell nuclei in the early stage and the process of experiments. Not only improves the experimental efficiency and shortens the experimental time, but also is beneficial to the smooth performance of the experiment.

Description

High-efficient extraction element of whole genome of rice
Technical Field
The invention relates to the technical field of biological equipment, in particular to a rice whole genome efficient extraction device.
Background
Application number 201811174704.5 discloses a rice genome DNA group extraction element, including box, centrifugal test tube, water bath chamber and casing, the inside of casing evenly is fixed with connecting gear through the second connecting axle, and all is fixed with connecting gear through first connecting axle between the connecting gear, intermeshing between runner and the connecting gear, control panel is installed to one side of casing bottom, and control panel's internally mounted has the singlechip, one side on casing top is provided with the carousel, and one side on carousel top is fixed with the crank through the pivot, fixed connection between the bottom of carousel and the second connecting axle top of one side inside the casing, the below of casing evenly is provided with centrifugal test tube. According to the invention, the pistons are arranged in the mixing cavity, the push rods are arranged at the bottom ends of the pistons, and the screen mesh is inlaid at the top ends between the inner parts of the mixing cavity, so that solid impurities in the mixed liquid can be removed.
In the rice experiment process, a plurality of groups of samples are prepared for one time, so that enough comparison groups are ensured, and meanwhile, even the same operation steps are adopted, the condition of unsuccessful extraction exists, so that the rice samples need to be frequently subjected to gene extraction during the experiment;
In the gene extraction step, the key step is to break the cell membrane of the rice, and usually, the cell membrane is broken by adopting an eccentric rotation centrifugation mode, but the equipment in the patent cannot achieve the eccentric rotation centrifugation effect, so that the cell membrane is poor in breaking effect and can possibly cause DNA extraction failure, and meanwhile, the equipment and the parts in the patent are arranged separately, so that the occupied area is large.
Disclosure of Invention
The invention aims to provide a rice whole genome efficient extraction device.
In order to achieve the above purpose, the present invention adopts the following technical scheme: an efficient extraction device for whole genome of rice, comprising:
The main body comprises a workbench and a fixed container, wherein the workbench provides an installation space for other parts, and the fixed container is arranged on the workbench;
The driving mechanism comprises a centrifugal driving motor, a cross driving shaft, a transmission sleeve and a piston, wherein the centrifugal driving motor is arranged at the top of the workbench, the output shaft of the centrifugal driving motor is fixedly connected with the cross driving shaft through a bolt, the transmission sleeve is sleeved on the outer side of the cross driving shaft, a cross through groove which is anastomotic with the cross driving shaft in shape is formed in the transmission sleeve, the transmission sleeve moves up and down along the cross driving shaft, and the piston is fixedly connected with the bottom end of the transmission sleeve;
The extraction mechanism comprises a centrifugal pressurizing container, the centrifugal pressurizing container is used for containing rice experimental samples and extracting solution, and the centrifugal pressurizing container is rotatably arranged on the inner side of the fixed container.
Preferably, the piston consists of a cylinder and a round table, and the side wall of the cylinder part of the piston is integrally connected with a bulge.
Preferably, the centrifugal pressurizing container is provided with a cavity on the inner side, a spiral groove is formed in the inner wall of the cavity, and the protrusions are matched with the spiral groove.
Preferably, the driving mechanism further comprises a rotating screw rod, a connecting rod and a driving wheel, the rotating screw rod is rotatably arranged on the front side of the workbench, the driving wheel with the same center is arranged on the rotating screw rod, the driving wheel is driven by a motor through a driving belt, the motor is driven by the driving wheel and the rotating screw rod through the driving belt, the rotating screw rod penetrates through the connecting rod, and the other end of the connecting rod is fixedly connected with the driving sleeve.
Preferably, the centrifugal pressurizing container is placed on the interlayer on the inner side of the fixed container, the interlayer on the inner side of the fixed container is provided with a bearing, and the centrifugal pressurizing container is rotationally connected with the fixed container through the bearing.
Preferably, the extraction mechanism further comprises a first guide pipe, elastic sheets, a ring sleeve and a spring, a through hole is formed in the center of the bottom of the centrifugal pressurizing container and is communicated with the cavity, the ring sleeve is installed on the inner side of the through hole, the spring is arranged at the bottom of the ring sleeve, a plurality of fan-shaped elastic sheets are installed on the inner wall of the ring sleeve, and a conical sealing valve is formed by the elastic sheets.
Preferably, the first end of the spring is connected with the annular sleeve, and the second end of the spring is connected with the inner wall of the through hole of the centrifugal pressurizing container.
Preferably, the interlayer on the inner side of the fixed container is provided with a first conduit corresponding to the through hole, the bottom of the fixed container is provided with a test tube, the inner wall of the fixed container is provided with a clamp for fixing the test tube, and the fixed container is provided with a bin gate.
Preferably, the bottom of the piston has a second conduit which cooperates with the collar.
Preferably, the inner side of the elastic sheet is provided with a metal strip, and the metal strip is made of titanium.
The invention has at least the following beneficial effects:
This design adopts the mode that the cooperation of rotating centrifugation increased atmospheric pressure, can make the rice cell membrane fully break, conveniently draws the rice cell nucleus before the experiment and in-process high efficiency, makes things convenient for going on smoothly of experiment for the experiment progress, and the processing part of this device is on same vertical straight line simultaneously, reduces area, saves the precious area in laboratory.
The driving mechanism of this design, when realizing rotatory, can also realize the effect of vertical lift, both satisfied the requirement that the cooperation of rotation centrifugation increased the atmospheric pressure, simple structure uses the maintenance cost low simultaneously.
Through setting up the elastic flake that has the metal strip, can guarantee centrifugal leakproofness, also can satisfy automatic opening when the pressurized threshold value simultaneously, realize extracting solution and DNA's discharge to follow-up research and observation of being convenient for.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic illustration of the present invention;
FIG. 2 is a schematic illustration of a piston of the present invention;
FIG. 3 is a cross-sectional view of a centrifugal boost container of the present invention;
FIG. 4 is an enlarged schematic view of the portion A of the present invention;
FIG. 5 is a schematic view of a second conduit according to the present invention;
fig. 6 is a cross-sectional view of an elastic sheet of the present invention.
In the figure: 100. a main body; 101. a work table; 102. a fixed container; 103. a bin gate; 104. a test tube; 105. a clamp; 200. a driving mechanism; 201. a centrifugal drive motor; 202. a cross drive shaft; 203. rotating the screw rod; 204. a connecting rod; 205. a driving wheel; 206. a drive sleeve; 207. a piston; 208. a protrusion; 209. a second conduit; 300. an extraction mechanism; 301. a centrifugal pressurizing container; 302. a bearing; 303. a first conduit; 304. a spiral groove; 305. a cavity; 306. an elastic sheet; 307. a ring sleeve; 308. a spring; 309. a metal strip.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Referring to fig. 1 to 6, a rice whole genome efficient extraction apparatus comprises:
A main body 100, the construction of which includes a table 101 and a fixed container 102. The design of the table 101 is intended to provide sufficient installation space for other components. On the table 101 we mount a fixed container 102, the bottom of which houses a test tube 104. To ensure the stability of the test tube 104, we mount clamps 105 on the inner wall of the fixed container 102, dedicated to fixing the test tube 104. In addition, the fixed container 102 is also provided with a bin gate 103 for maintenance and operation;
The driving mechanism 200 comprises a centrifugal driving motor 201, a cross driving shaft 202, a transmission sleeve 206 and a piston 207, wherein the centrifugal driving motor 201 is arranged at the top of the workbench 101, an output shaft of the centrifugal driving motor 201 is fixedly connected with the cross driving shaft 202 through bolts, the transmission sleeve 206 is sleeved outside the cross driving shaft 202, a cross through groove which is matched with the cross driving shaft 202 in shape is formed in the transmission sleeve 206, the transmission sleeve 206 moves up and down along the cross driving shaft 202, the piston 207 is fixedly connected with the bottom end of the transmission sleeve 206, the piston 207 consists of a cylinder and a round table, a bulge 208 is integrally connected to the side wall of the cylindrical part of the piston 207, a cavity 305 is formed in the inner side of the centrifugal pressurizing container 301, a spiral groove 304 is formed in the inner wall of the cavity 305, the bulge 208 is mutually matched with the spiral groove 304, and the section of the bulge 208 is equal to the section of the spiral groove 304, so that the tightness is ensured;
The extraction mechanism 300 includes a centrifugal pressurizing container 301, a first conduit 303, an elastic sheet 306, a collar 307, and a spring 308. The centrifugal pressurizing container 301 is mainly used for storing rice experimental samples and extracting solutions. The container is secured by a rotational mounting to the inside of the container 102 and has a through hole in the center of its bottom which communicates with the cavity 305. The inner side of the through hole is provided with a ring sleeve 307, and the bottom of the ring sleeve 307 is provided with a spring 308. The inner wall of the collar 307 is also provided with a plurality of sector-shaped elastic lamellae 306 which form a conical sealing valve.
Further, the driving mechanism 200 mainly comprises a rotating screw 203, a connecting rod 204 and a driving wheel 205. The rotary screw 203 is mounted on the front side of the table 101 and is provided with a concentric driving wheel 205 thereon. The driving wheel 205 is connected with the motor through a driving belt, so that power transmission is realized. The motor drives the rotation of the driving wheel 205 and the rotation screw 203 through the driving belt. In addition, the rotating screw 203 penetrates the connecting rod 204, and the other end of the connecting rod 204 is fixedly connected with the transmission sleeve 206.
Further, the centrifugal boost container 301 is placed on the inside compartment of the stationary container 102, which is provided with bearings 302. Through this bearing 302, the centrifugal boost container 301 is in rotational connection with the stationary container 102. The inner ring of the bearing 302 is sleeved on the interlayer, and the annular groove at the bottom of the centrifugal booster container 301 is sleeved on the bearing 302 and is fixedly connected with the outer ring of the bearing 302.
Further, a first end of the spring 308 is connected to the collar 307, and a second end of the spring 308 is connected to the inner wall of the through hole of the centrifugal booster container 301.
Further, the inner partition of the fixed container 102 is provided with a first conduit 303 corresponding to the through hole.
Further, the bottom of the piston 207 has a second conduit 209, the second conduit 209 being used in cooperation with the collar 307.
Further, the inner side of the elastic sheet 306 is provided with a metal strip 309, the metal strip 309 is made of titanium, and the elastic sheet 306 is made of rubber.
Further, the metal strips 309 are distributed near the center of the circle.
The scheme comprises the following working processes: placing the ground particles and extracting solution in a centrifugal pressurizing container 301, then placing the centrifugal pressurizing container 301 in a fixed container 102, rotating through a rotating screw rod 203 to drive a piston 207 to descend until a bulge 208 of the piston 207 falls into a spiral groove 304, stopping rotating through the rotating screw rod 203, keeping the height of the piston 207 unchanged, driving a transmission sleeve 206 and the piston 207 to rotate through the centrifugal pressurizing container 301, namely, enabling the bulge 208 to contact the spiral groove 304 and drive the centrifugal pressurizing container 301 to rotate, realizing rotating centrifugation, driving the rotating screw rod 203 to drive the piston 207 to move downwards in the process of moving downwards, and increasing the pressure in a cavity 305 in the process of moving downwards, so that the membrane of the rice is broken;
Or after rotation and centrifugation, the rotating screw rod 203 is driven to drive the piston 207 to move downwards, so that the pressure intensity in the cavity 305 is increased in the downward movement process, and the effect of helping rupture of rice cell membranes is achieved;
When the pressure reaches the threshold value, the elastic sheet 306 is opened, the extracting solution and DNA can be conveyed into the test tube 104 through the through hole, the metal strip 309 is added, the metal strip 309 increases the deformation resistance of the elastic sheet 306, the deformation of the elastic sheet 306 during centrifugal rotation can be avoided, but the metal strip 309 also has toughness, and when the pressure reaches the threshold value, the extracting solution can be automatically opened and output.
When the piston 207 descends to the lowest position, the second conduit 209 presses the ring 307 downwards, the elastic sheet 306 is lifted upwards by the first conduit 303, and at the moment, the elastic sheet 306 is completely opened, so that the DNA is conveniently discharged completely;
Since the metal strips 309 are distributed near the center of the circle, no metal strips 309 are arranged at the root of the elastic sheet 306, and the situation that the elastic sheet 306 cannot be completely reset due to metal fatigue is avoided.
The working process can be as follows: the design scheme adopts a combination mode of rotary centrifugation and air pressure enhancement, and aims to fully rupture the cell membrane of the rice cells. The design is helpful for rapidly and efficiently extracting rice cell nuclei in the early stage and the experimental process of the experiment, thereby ensuring the smooth performance of the experiment and effectively improving the experimental efficiency. In addition, the processing components of the device are all positioned on the same vertical line, so that the space occupied by the device is reduced, and the area use of a laboratory is further optimized.
Regarding the driving mechanism, the design realizes the rotation function and simultaneously has the vertical lifting function. The design not only meets the requirements of rotary centrifugation and air pressure enhancement, but also has a simple structure, and greatly reduces the use and maintenance cost.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention, which is defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (10)

1. The utility model provides a high-efficient extraction element of whole genome of rice, its characterized in that includes:
The main body (100), the main body (100) comprises a workbench (101) and a fixed container (102), the workbench (101) provides installation space for other components, and the fixed container (102) is installed on the workbench (101);
The driving mechanism (200) comprises a centrifugal driving motor (201), a cross driving shaft (202), a transmission sleeve (206) and a piston (207), wherein the centrifugal driving motor (201) is installed at the top of the workbench (101), an output shaft of the centrifugal driving motor (201) is fixedly connected with the cross driving shaft (202) through bolts, the transmission sleeve (206) is sleeved on the outer side of the cross driving shaft (202), a cross through groove which is anastomotic with the cross driving shaft (202) in shape is formed in the transmission sleeve (206), the transmission sleeve (206) moves up and down along the cross driving shaft (202), and the piston (207) is fixedly connected with the bottom end of the transmission sleeve (206);
The extraction mechanism (300), extraction mechanism (300) include centrifugal booster container (301), centrifugal booster container (301) is used for holding rice experiment sample and extract, centrifugal booster container (301) rotate the inboard of installing in fixed container (102).
2. The efficient rice genome-wide extraction device according to claim 1, wherein the piston (207) comprises a cylinder and a circular truncated cone, and a protrusion (208) is integrally connected to the side wall of the cylindrical portion of the piston (207).
3. The efficient rice genome-wide extraction device according to claim 2, wherein a cavity (305) is formed inside the centrifugal pressurizing container (301), a spiral groove (304) is formed in the inner wall of the cavity (305), and the protrusion (208) is matched with the spiral groove (304).
4. The efficient rice genome-wide extraction device according to claim 1, wherein the driving mechanism (200) further comprises a rotating screw rod (203), a connecting rod (204) and a driving wheel (205), the rotating screw rod (203) is rotatably mounted on the front side of the workbench (101), the concentric driving wheel (205) is mounted on the rotating screw rod (203), the driving wheel (205) is driven by a motor through a driving belt, the motor is driven by the driving wheel (205) and the rotating screw rod (203), the rotating screw rod (203) penetrates through the connecting rod (204), and the other end of the connecting rod (204) is fixedly connected with a driving sleeve (206).
5. The efficient rice genome-wide extraction device according to claim 1, wherein the centrifugal pressurizing container (301) is placed on an inner interlayer of the fixed container (102), the inner interlayer of the fixed container (102) is provided with a bearing (302), and the centrifugal pressurizing container (301) is rotatably connected with the fixed container (102) through the bearing (302).
6. The efficient rice genome-wide extraction device according to claim 1, wherein the extraction mechanism (300) further comprises a first conduit (303), an elastic sheet (306), a ring sleeve (307) and a spring (308), a through hole is formed in the central position of the bottom of the centrifugal pressurizing container (301) and communicated with the cavity (305), the ring sleeve (307) is installed inside the through hole, the spring (308) is arranged at the bottom of the ring sleeve (307), a plurality of fan-shaped elastic sheets (306) are installed on the inner wall of the ring sleeve (307), and the plurality of elastic sheets (306) form a conical sealing valve.
7. The efficient rice genome-wide extraction device of claim 6, wherein a first end of the spring (308) is connected with the collar (307), and a second end of the spring (308) is connected with the inner wall of the through hole of the centrifugal pressurizing container (301).
8. The efficient rice genome-wide extraction device according to claim 1, wherein a first conduit (303) corresponding to the through hole is arranged on an inner interlayer of the fixed container (102), a test tube (104) is arranged at the bottom of the fixed container (102), a clamp (105) for fixing the test tube (104) is arranged on the inner wall of the fixed container (102), and a bin gate (103) is arranged on the fixed container (102).
9. The efficient rice genome-wide extraction device according to claim 1, characterized in that the bottom of the piston (207) is provided with a second conduit (209), said second conduit (209) being used in cooperation with the collar (307).
10. The rice whole genome efficient extraction device according to claim 1, wherein the inner side of the elastic sheet (306) is provided with a metal strip (309), and the metal strip (309) is made of titanium.
CN202410396610.1A 2024-04-03 2024-04-03 High-efficient extraction element of whole genome of rice Pending CN117987245A (en)

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Application Number Priority Date Filing Date Title
CN202410396610.1A CN117987245A (en) 2024-04-03 2024-04-03 High-efficient extraction element of whole genome of rice

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Application Number Priority Date Filing Date Title
CN202410396610.1A CN117987245A (en) 2024-04-03 2024-04-03 High-efficient extraction element of whole genome of rice

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CN117987245A true CN117987245A (en) 2024-05-07

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998348A (en) * 2018-10-09 2018-12-14 广东海洋大学 A kind of paddy gene DNA group extraction element
CN111304056A (en) * 2020-03-11 2020-06-19 李容尔 Cell sample draws partial shipment device
CN116019150A (en) * 2022-12-19 2023-04-28 广西凌云一尖茶业有限公司 Tea dryer integrating screening and tea processing method
CN117804825A (en) * 2024-03-01 2024-04-02 黑龙江省农业科学院水稻研究所 Remote control automatic sampling device in paddy field

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998348A (en) * 2018-10-09 2018-12-14 广东海洋大学 A kind of paddy gene DNA group extraction element
CN111304056A (en) * 2020-03-11 2020-06-19 李容尔 Cell sample draws partial shipment device
CN116019150A (en) * 2022-12-19 2023-04-28 广西凌云一尖茶业有限公司 Tea dryer integrating screening and tea processing method
CN117804825A (en) * 2024-03-01 2024-04-02 黑龙江省农业科学院水稻研究所 Remote control automatic sampling device in paddy field

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