CN117956903A - Modified antimicrobial peptides - Google Patents

Abstract

Antimicrobial defensin peptide variants comprising a modified C-terminal fragment of defensin and nucleic acids encoding the same are disclosed. Also disclosed are compositions comprising these defensin variant peptides and methods of their use for controlling microbial infections and feed and food contamination in plant and vertebrate subjects.

Description

Modified antimicrobial peptides

The inventors: dilip M Shah and MEENAKSHI TETORYA

Cross Reference to Related Applications

The present application claims the benefit of U.S. patent application Ser. No. 63/365,928 filed on 6 months 2022 and U.S. patent application Ser. No. 63/202,559 filed on 16 months 2021, each of which is incorporated herein by reference in its entirety.

Government support statement

The invention was completed with government support under national science foundation EAGER awards number 1955461 issued by the national science foundation (National Science Foundation). The government has certain rights in the invention.

Sequence listing

The sequence listing contained in the file named "P13511WO00_st25" was measured as 294,870 bytes in the Windows operating system, created at month 6, 15 of 2022 and submitted electronically with this document, the entire contents of which are incorporated herein by reference.

Background

Protection of important agricultural crops from pathogenic microorganisms (e.g., fungi or oomycetes) is critical to improving crop yield. Microbial infection is a particular problem in humid climates and can become a major problem during storage of crops, where such infection can lead to spoilage of food or feed products and contamination by microbial toxins. Unfortunately, modern planting methods, harvesting and storage systems may promote plant pathogen infection.

Control of plant pathogens becomes more complex due to the need to control multiple microorganisms of different genus simultaneously. For example microorganisms such as Alternaria; genus aschersonia; the genus myceliophthora (Aphenomyces); botrytis; cercospora species; genus Cephalosporium; the genus chromatophora; powdery mildew genus; fusarium genus; top capsule genus (Gaeumanomyces); helminthosporium (Helminthosporium); the genus Sphaeromyces, the genus Eichhornia; the megabase shell belongs to the genus; the genus Conyza; the genus downy mildew; phoma genus; the genus Rumex, oenospora (Phymatotrichum); phytophthora genus; the genus Plasmodium; the cross-hair single-capsule shell belongs to the genus; puccinia spp; pythium species; the genus Nuclear cavity; pyricularia genus; rhizoctonia genus; sclerotium genus; sclerotinia; the genus aschersonia; rhizopus sp; the crochet hook shell belongs to the genus; the genus Armillariella; and Verticillium species are well known plant pathogens.

Certain microorganisms (e.g., fungi, including mold, yeast, and bimodal fungi, or oomycetes) can also be pathogenic to a variety of vertebrates including humans, livestock, companion animals, fish, and the like. Microorganisms including dermatophytes (dermatophytes), aspergillus, candida, cryptococcus, coccidioidomycosis, penicillium, rhizopus, lepidomycosis, candidiasis, phialides, rhizomucor, comycetes (Syncephalostrum), colestuary, actinomucor, pythium, fusarium, histoplasma, or blastomycosis species are also important vertebrate pathogens.

A group of proteins known as defensins have been shown to inhibit plant pathogens. Defensins have previously been identified as small cysteine-rich peptides of about 45-54 amino acids, constituting an important component of plant innate immunity (Thomma et al, 2002; lay and Anderson,2005; vriens et al, 2014). Defensins are widely distributed in plants and vary widely in their amino acid composition. However, they all have a compact shape, stabilized by four or five intramolecular disulfide bonds. Phytoalexins have previously been characterized as comprising a conserved gamma core peptide comprising a conserved GXCX-9C (where X is any amino acid) sequence (Sagaram et al, 2011; lacarda et al, 2014). The three-dimensional structure of the gamma core peptide previously characterized consisted of two antiparallel beta-sheets, interpolating the corner region (supra). The antimicrobial activity of certain defensins is related to the presence of positively charged amino acid residues in the gamma core peptide (Spelbrink et al, plant Physiol. [ Plant Physiol ],2004; sagaram et al, 2013).

The role of plant defensins in plant defense has been widely studied. Some plant defensins inhibit the growth of a variety of microorganisms at micromolar concentrations (Broekaert et al, 1995; broekaert et al, 1997;da Silva Conceicao and Broekaert, 1999), which when expressed in transgenic plants confer strong resistance to microbial pathogens (da Silva Conceicao and Broekaert,1999; thomma et al, 2002; lay and Anderson, 2005). When pure protein is added to in vitro antimicrobial assay medium, two cysteine-rich small proteins, rs-AFPl and Rs-AFP2, isolated from radish seeds inhibit the growth of many pathogenic microorganisms (U.S. Pat. No. 5,538,525). Transgenic tobacco plants containing genes encoding Rs-AFP2 protein were found to be more resistant to microbial attack than non-transformed plants.

Defensin genes have also been identified in alfalfa, a leguminous plant (Hanks et al 2005). Cloned MtDef2 protein has been demonstrated to have little antimicrobial activity by in vitro experiments (Spelbrink et al, 2004). The medicago truncatula defensin proteins MtDef4 (U.S. patent No. 7,825,297; incorporated herein by reference in its entirety) and MtDef5 (WO 2014179260 and U.S. patent application publication No. 20160208278; both incorporated herein by reference in their entirety) have antimicrobial activity. The C-terminal 16 amino acid GMA4-C peptide of MtDef4 defensin protein inhibits Fusarium graminearum at concentrations as low as 3. Mu.M (Sagaram et al, 2011).

Plant defensins with potent in vitro antifungal activity generally do not confer effective disease resistance to plants. This limits their commercial development as antifungal agents for transgenic crops. Antifungal plant defensins are generally cationic, with cationic residues in their sequence thought to initiate a pathway through the fungal cell envelope through electrostatic interactions with anionic fungal cell membranes (Kerenga et al, 2019). Potassium (k+) is a necessary macronutrient and is also the most abundant cation in plants. The concentration of k+ in plant cytoplasm was always between 100 and 200mM (Shabala and Pottosin, 2010) and in apoplast was always between 10 and 200mM (White and Karley, 2010). Calcium is an essential secondary micronutrient and may range in concentration from 0.1% to 6% of the dry weight of the plant (Broadley et al, 2003). The concentration of sodium (Na+) in plants ranges from 0.001% to 8% (Marschner, 1995). Na+ is a trace nutrient essential to plants in saline soil.

Many plant defensins characterized so far lose their antifungal activity at high concentrations of monovalent and divalent cations (e.g., 100mM KC1 or 2mM CaCl ₂). However, in the presence of 100mM NaCl, maize plant defensin ZmD32, which predicts a charge of +10.1 at pH 7, showed inhibitory activity against Candida species and E.coli, whereas in the presence of 100mM NaCl, nicotiana benthamiana (Nicotiana benthamiana) plant defensin NbD6, which predicts a charge of +7.6 at pH 7, showed inhibitory activity against Candida albicans (Kerenga et al, 2019).

Disclosure of Invention

There is provided a peptide comprising the sequence XGXCXGFXXXX (F/W/Y) XXXXC (SEQ ID NO: 1), wherein the peptide does not comprise the corresponding full-length sequence of the defensin peptide of SEQ ID NO:8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41; optionally wherein the peptide comprises the modified gamma core consensus sequence of GXCX-8 (F/W/Y) (F/W/Y) (F/W/Y) shown in SEQ ID NO:33, GXCX-9 (F/W/Y) (F/W/Y) (F/W/Y), GXCX3-8 (F/W/Y) (SEQ ID NO: 43), or GXCX3-9 (F/W/Y) (SEQ ID NO: 44) shown in SEQ ID NO: 34; and/or optionally wherein the C-terminal cysteine residue or C-terminal amino acid residue is amidated. A peptide is provided having at least 75%, 82% or 94% sequence identity over the full length of any one of SEQ ID nos. 3, 4, 5 or 6, wherein the peptide is not identical to SEQ ID No. 8, optionally wherein any amino acid substitution in said sequence increases or maintains the net positive charge of the peptide and/or increases or maintains the hydrophobicity of the peptide at neutral pH, and optionally wherein the C-terminal cysteine residue is amidated. A peptide is provided having at least 75%, 82% or 94% sequence identity over the full length of SEQ ID NOs 7, 12, 13, 14, 15, 17-20, 22, 23, 25, 26, 28, 29, 31 and 32, wherein the peptide is not identical to SEQ ID NOs 8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41, optionally wherein the peptide of SEQ ID NOs 5, 13, 14, 15, 18, 19, 20, 22, 23, 26, 28, 29, 31 or 32 comprises a disulfide bond between two cysteine residues, and optionally wherein any substitution increases or maintains the net positive charge of the peptide at neutral pH and/or increases or maintains the hydrophobicity of the peptide, and optionally wherein the C-terminal cysteine residue or C-terminal amino acid residue is amidated. Provided is a defensin C-terminal peptide variant comprising conserved C1 and C4 cysteine residues corresponding to the N-terminal and C-terminal cysteines of a reference defensin C-terminal peptide wherein the conserved C2 and C3 cysteine residues of the reference defensin C-terminal peptide are independently substituted with tryptophan, tyrosine, phenylalanine, leucine, valine, isoleucine or methionine; and optionally wherein the defensin peptide variant has a net positive charge of at least 3, 3.5, 4, 5 or 6 and a hydrophobic amino acid content of at least 18%. A C-terminal defensin peptide variant is provided comprising a modified gamma core consensus sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、 or GXCX-10 (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (SEQ ID NO: 52). In certain embodiments, the foregoing peptides exhibit antimicrobial activity, wherein the antimicrobial activity is optionally one or more of antifungal or antibacterial activity. Also provided are compositions comprising any of the foregoing peptides and an agriculturally, pharmaceutically or veterinarily practically acceptable carrier, diluent or excipient. Also provided are compositions comprising any of the foregoing peptides and a carrier, diluent or excipient for use in treating, preventing or inhibiting a microbial infection in a subject in need thereof.

Also provided are methods for: (i) Preventing or reducing damage to crops by plant pathogenic microorganisms, or (ii) preventing contamination of plants, plant parts, seeds, feed obtained therefrom, or food products obtained therefrom by undesired microorganisms, comprising the step of contacting a plant, plant seed, or other part of said plant with an effective amount of any of the foregoing compositions, wherein the composition optionally comprises an agriculturally acceptable carrier, diluent, or excipient.

Also provided are plant parts, including seeds, at least partially coated with any of the foregoing compositions, wherein the composition optionally comprises an agriculturally acceptable carrier, diluent, or excipient.

Also provided are medical devices comprising a device and the aforementioned composition, wherein the device comprises at least one surface that is topically coated and/or impregnated with the composition, wherein the composition optionally comprises a pharmaceutically or veterinarily practically acceptable carrier, diluent or excipient.

Also provided are methods for treating, preventing, or inhibiting a microbial infection in a subject in need thereof, comprising administering to the subject an effective amount of any of the foregoing compositions, wherein the composition optionally comprises a pharmaceutically or veterinarily practically acceptable carrier, diluent, or excipient. There is provided a use of any of the foregoing compositions in a method of treating, preventing or inhibiting a microbial or yeast infection in a subject in need thereof. Also provided is the use of any of the foregoing first antimicrobial peptides or proteins in the manufacture of a medicament or composition for inhibiting microbial or yeast infection in a subject in need thereof.

Recombinant polynucleotides are provided comprising polynucleotide peptides comprising any of the foregoing peptides, wherein the polynucleotide encoding an antimicrobial peptide is operably linked to a polynucleotide comprising a promoter heterologous to the polynucleotide encoding the first antimicrobial peptide, optionally wherein any amino acid substitution in the sequence increases or maintains the net positive charge and/or hydrophobicity of the peptide.

A plant nuclear or plastid genome is provided comprising a polynucleotide encoding an antimicrobial peptide comprising any of the foregoing peptides, wherein the polynucleotide is heterologous to the nuclear or plastid genome, and wherein the polynucleotide is operably linked to an endogenous promoter of the nuclear or plastid genome.

Cells, plants and plant parts including seeds comprising the aforementioned recombinant polynucleotides or genomes are provided.

Drawings

FIGS. 1A and 1B show instillation inoculation assays for N.benthamiana. Four week old benthamia tobacco was used for drip inoculation assays against botrytis cinerea using different concentrations of GMA4C peptide. Illustrations and pictures of instillation inoculation assays were taken under white light and after 48h incubation using CropReporter. The damaged area is represented in red in CropReporter images. The data for the GMA4C_AC wild-type control (SEQ ID NO: 2) is in the leftmost panel (labeled "GMA 4C"), the data for GMA4c_V1A (SEQ ID NO: 3) is in the 2 nd panel from the left, the data for GMA4c_V2A (SEQ ID NO: 4) is in the 3 rd panel from the left, and the data for GMA4c_V3A (SEQ ID NO: 5) is in the 4 th panel from the left. Lesions were measured after 1b,48h using ImageJ software and lesion sizes are shown in the figure.

Figure 2 shows an alignment of a non-limiting subset of reference defensin C-terminal peptides comprising conserved C1, C2, C3 and C4 cysteines (bold). The conserved gamma core peptide is underlined.

Figure 3 shows a non-limiting subset of reference defensin C-terminal peptides comprising conserved C1, C2, C3 and C4 cysteines (bold) with an alignment of gamma core consensus sequences and various modified gamma core consensus sequences provided herein. The conserved gamma core peptide is underlined and absent in the modified gamma core consensus peptide.

Figure 4 shows a data table of antimicrobial activity of various GMA4C defensin peptide variants.

Fig. 5A, B. FIG. 5A, B shows the results of instillation inoculation assays, which show the antifungal activity of GMA4C_V9 (SEQ ID NO: 581) and GMA4C_V10 peptide (SEQ ID NO: 582). Gma4c_v10 completely eliminates gray mold symptoms at 6 μm. In FIG. 5B, the leftmost bar is a NO-treatment control ("Botrytis cinerea"), the middle bar is GMA4C_V9 (SEQ ID NO: 581) treatment, and the rightmost bar is GMA4C_V10 (SEQ ID NO: 582) treatment. At 3 μM and 1.5 μM, gma4c_v10 was more effective at reducing gray mold symptoms than gma4c_v9.

FIG. 6A, B shows the results of instillation inoculation assays, which show the antifungal activity of GMAOe C_WT (SEQ ID NO: 577), GMAOe C_V3 (SEQ ID NO: 578), and GMAOe C_V4 (SEQ ID NO: 579). In FIG. 6B, the leftmost bar is no treatment control ("Botrytis cinerea"), the 2 nd bar from the left is GMAOe C_WT treatment, the 3 rd bar from the left is GMAOe C_V3 treatment, and the 4 th bar from the left is GMAOe C_V4 treatment. GMAOe C_v3 and GMAOe C_v4 at 3 μM and 6 μM completely eliminated gray mold symptoms, but GMAOe C_WT was only effective at 6 μM. GMAOe c_v3 was more effective than GMAOe c_v4 or GMAOe c_wt at a concentration of 1.5 μm.

FIG. 7A, B shows the results of instillation inoculation assay, which shows the antifungal activity of GMA1C_V1 (SEQ ID NO: 583) and GMA1C_V2 (SEQ ID NO: 584) against Botrytis cinerea. In FIG. 7B, the leftmost bar is a NO-treatment control ("Botrytis cinerea"), the middle bar is GMA1C_V1 (SEQ ID NO: 583) treatment, and the rightmost bar is GMA1C_V2 (SEQ ID NO: 584) treatment.

Detailed Description

Definition of the definition

The term "and/or" as used herein is to be taken as a specific disclosure of each of two specified features or components with or without another specified feature or component. Thus, the term "and/or" as used in phrases such as "a and/or B" herein is intended to include "a and B", "a or B", "a" (alone) and "B" (alone). Likewise, the term "and/or" as used in a phrase such as "A, B, and/or C" is intended to encompass each of the following embodiments: A. b, and C; A. b, or C; a or C; a or B; b or C; a and C; a and B; b and C; a (alone); b (alone); and C (alone).

As used herein, the term "corresponding (correspond, corresponding)" and the like, when used in the context of amino acid positions, mutations, and/or substitutions of any given peptide (e.g., a defensin variant peptide) relative to a reference peptide sequence (e.g., a reference defensin C-terminal peptide sequence including SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, or 89-122) all refer to an amino acid residue in a given peptide sequence that has the same position in the given peptide as a residue in a reference amino acid sequence when the given peptide is aligned with the reference sequence. In certain embodiments, the alignment is an alignment of 4 conserved cysteine residues of the defensin C-terminal peptide of the defensin variant peptide and the reference defensin C-terminal peptide sequence (e.g., as shown in fig. 2 and 3).

As used herein, the term "comprising" shall be construed to have at least the features to which they refer without excluding any further unspecified features.

When a term is provided in the singular, other embodiments described by the plural of that term are also provided.

As used herein, the phrase "antimicrobial peptide" refers to a peptide that exhibits any one or more of the following characteristics: inhibit the growth of microbial cells, kill microbial cells, disrupt or delay a stage of a microbial life cycle (e.g., spore germination, sporulation, or mating), and/or disrupt microbial cell infection, penetration, or spread within plants or other susceptible subjects, including humans, livestock, poultry, fish, or companion animals (e.g., dogs or cats).

As used herein, the terms "acidic" or "anionic" are used interchangeably to refer to amino acids, such as aspartic acid and glutamic acid.

As used herein, the term "amino acid" refers to an organic compound containing amino (-NH ₃) and carboxylate (-CO ₂) functional groups as well as side chains (R groups) unique to each amino acid. In certain instances, amino acid residues in a polypeptide are represented herein by single letter amino acid codes as follows: g-glycine (Gly); p-proline (Pro); a-alanine (Ala); v-valine (Val); l-leucine (Leu); i-isoleucine (Ile); m-methionine (Met); c-cysteine (Cys); f-phenylalanine (Phe); y-tyrosine (Tyr); w-tryptophan (Trp); h-histidine (His); k-lysine (Lys); r-arginine (Arg); q-glutamine (Gln); n-asparagine (Asn); e-glutamic acid (Glu); d-aspartic acid (Asp); s-serine (Ser); or T-threonine (Thr).

As used herein, the terms "basic" or "cationic" are used interchangeably to refer to amino acids such as arginine, histidine, and lysine.

As used herein, the phrase "cationically tolerant" refers to a defensin peptide or a defensin peptide variant that exhibits comparable in vitro antifungal or antimicrobial activity, or a reduction of no more than about 1.5-fold, 2-fold, 3-fold, or 4-fold in vitro antifungal or antimicrobial activity in the presence of l00 mM KCl or l00 mM NaCl as compared to the antifungal activity of the defensin peptide or defensin peptide variant in the absence of KC1 or NaCl.

As used herein, the phrase "consensus sequence" refers to an amino acid, DNA, or RNA sequence produced by aligning two or more homologous sequences and deriving a new sequence having a conserved or a set of alternative amino acid, deoxyribonucleic acid, or ribonucleic acid residues of the homologous sequence at each position in the produced sequence.

As used herein, the phrase "combating microbial damage," "combating or controlling microbial damage," or "controlling microbial damage" refers to reducing damage to a crop plant or crop plant product caused by infection by a microbial pathogen. More generally, these phrases refer to reducing the adverse effects caused by the presence of pathogenic microorganisms in crop plants. Adverse effects of microbial growth are understood to include any type of plant tissue damage or necrosis, any type of plant yield reduction, any reduction in the value of the crop plant product, and/or the production of undesirable microbial metabolites or microbial growth byproducts (including mycotoxins).

The phrase "defensin peptide" is used herein to refer to a peptide comprising a conserved gamma core peptide. Phytoalexins have previously been characterized as comprising either a conserved GXCX-9C gamma core peptide sequence (SEQ ID NO: 9) where X is any amino acid residue (Lacerda et al) or a conserved GXCX3-10C variant gamma core peptide sequence (SEQ ID NO: 10) where X is any amino acid residue. In certain embodiments, the defensin peptides disclosed herein may also include a nonstandard defensin gamma core peptide comprising GXCX-12C (SEQ ID NO: 590) or GXCX-15C (SEQ ID NO: 591). Thus, as used in this disclosure, a plant defensin or a C terminal peptide comprising a fragment thereof may comprise a conserved GXCX3-9C (SEQ ID NO: 9), GXCX3-10C (SEQ ID NO: 10), GXCX3-12C (SEQ ID NO: 590) or GXCX3-15C (SEQ ID NO: 591) gamma core peptide sequence wherein X is any amino acid residue. Defensin peptides include proteins that are antimicrobial, permeabilize plasma membranes, bind phospholipids, bind sphingolipids, or exhibit any combination of these properties. Defensin peptides may be naturally occurring or non-naturally occurring (e.g., synthetic and/or chimeric).

The phrase "defensin peptide variant" is used herein to describe a modified defensin peptide comprising: (i) At least one amino acid substitution in the conserved gamma core peptide of SEQ ID NO 9 or 10 and the source defensin peptide; or (ii) a modified gamma core variant sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、 or GXCX3-10 (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (SEQ ID NO: 52). In certain embodiments, the defensin peptide variants provided herein are smaller than a full-length defensin peptide (e.g., comprising, consisting essentially of, or consisting of (i) 30 amino acid residues or less, or (ii) 15, 16, or 17 to 30 amino acid residues).

The phrase "reference defensin C-terminal peptide" is used herein to refer to a less than full length defensin peptide comprising a conserved GXCX-9 cγ core peptide sequence (SEQ ID NO: 9), a conserved GXCX3-10C variant γ core peptide sequence (SEQ ID NO: 10), a nonstandard GXCX3-12C (SEQ ID NO: 590) γ core sequence or a nonstandard GXCX-15C (SEQ ID NO: 591) γ core sequence, and two additional conserved cysteine residues at the C-terminus of the γ core peptide sequence, wherein the cysteine closest to the N-terminus of the reference defensin C-terminal peptide corresponds to the cysteine closest to the N-terminus of the γ core sequence of SEQ ID NO:9 or SEQ ID NO: 10. Examples of reference defensin C-terminal peptides include MtDef 4C-terminal peptide (SEQ ID NO: 8), mtDef5A C terminal peptide (SEQ ID NO: 16), mtDef5B C terminal peptide (SEQ ID NO: 41), mtDef6 peptide (SEQ ID NO: 21), oeDef C-terminal peptide (SEQ ID NO: 24), oeDef C-terminal peptide (SEQ ID NO: 27), sbDef C-terminal peptide (SEQ ID NO: 30), naD 1C-terminal peptide (SEQ ID NO: 37), rsAFP 2C-terminal peptide (SEQ ID NO: 38), dmAMP 1C-terminal peptide (SEQ ID NO: 39) and HsAFP (SEQ ID NO: 40). Other examples of reference defensin C-terminal peptides include SEQ ID NOS 89 to 122. Thus, the reference defensin C-terminal peptide comprises 4 conserved cysteine residues referred to herein as C1 (the N-terminal end in the peptide and gamma core peptide sequence), C2 (the C-terminal end of C1 and located at the gamma core peptide sequence), C3 (the C-terminal end of C2) and C4 (the C-terminal end of C3). Figure 2 shows an alignment of a non-limiting subset of reference defensin C-terminal peptides comprising a conserved gamma core peptide sequence and C1, C2, C3 and C4 cysteines. Figure 3 shows a non-limiting subset of reference defensin C-terminal peptides comprising conserved gamma core peptide sequences and C1, C2, C3 and C4 cysteines, gamma core consensus sequences comprising C1 and C2 cysteines, and an alignment of certain modified gamma core consensus sequences provided herein. Other reference defensin C-terminal peptides include those contained in the full length defensin peptides of SEQ ID NOS 123 to 434 and 436 to 576.

As used herein, the term "editing (edit, editing, edited, etc)" refers to the process or product of introducing insertions, deletions, and/or nucleotide substitutions into the genome. Such processes include methods of inducing homology-directed repair and/or non-homologous end joining at one or more sites in the genome.

The phrase "genetically edited plant" or "edited plant" is used herein to refer to a plant that includes one or more nucleotide insertions, deletions, substitutions, or any combination thereof in the genomic DNA of the plant. Such genetically edited plants may be constructed by techniques including CRISPR/Cas endonuclease-mediated editing, meganuclease-mediated editing, engineered zinc finger endonuclease-mediated editing, and the like.

The term "heterologous," as used herein in the context of a second polynucleotide operably linked to a first polynucleotide, refers to: (i) A second polynucleotide derived from a source different from the source of the first polynucleotide; (ii) A second polynucleotide derived from the same source as the first polynucleotide, wherein the first, second or both polynucleotide sequences are modified from their original form; (iii) A second polynucleotide arranged relative to the first polynucleotide in an order and/or orientation or genomic position or environment that differs from the order and/or orientation or genomic position or environment of the first and second polynucleotides in the naturally occurring cell; or (iv) the second polynucleotide is not present in a naturally occurring cell containing the first polynucleotide. Heterologous polynucleotides include polynucleotides that promote transcription (e.g., promoter and enhancer elements), transcript abundance (e.g., introns, 5'utr, and 3' utr), translation, or combinations thereof, as well as polynucleotides encoding defensin peptide variants or defensin peptides, spacer peptides, or localization peptides. In certain embodiments, the nuclear or plastid genome may comprise a first polynucleotide, wherein the second polynucleotide is heterologous to the nuclear or plastid genome. The "heterologous" polynucleotides that promote transcription, transcript abundance, translation, or a combination thereof, as well as polynucleotides encoding defensin peptide variants or defensin peptides, spacer peptides, or localization peptides, may be cell autologous, but arranged in an order and/or orientation or genomic position or environment different from that in naturally occurring cells. The polynucleotide that promotes transcription, transcript abundance, translation, or a combination thereof, and the polynucleotide encoding the defensin peptide variant or defensin peptide, spacer peptide, or localization peptide may be heterologous to another polynucleotide (when the polynucleotides are not operably linked to one another in a naturally occurring cell). Heterologous peptides or proteins include peptides or proteins not found in the cell or organism, as the cell or organism is naturally occurring. Thus, a heterologous peptide or protein includes a peptide or protein that is located at a subcellular location, extracellular location, or expressed in a tissue other than a subcellular location, extracellular location, or in a tissue where the peptide is found in a naturally occurring cell or organism. Heterologous polynucleotides include polynucleotides that are not found in a cell or organism when the cell or organism is naturally occurring.

As used herein, the phrases "inhibiting the growth of a plant pathogenic microorganism," "inhibiting the growth of a microorganism," and the like refer to any measurable reduction in microbial growth, including any measurable reduction in the number and/or extent of microbial cells, spores, conidia, or mycelium. As used herein, "inhibiting the growth of a plant pathogenic microorganism" is also understood to include any measurable reduction in the adverse effects caused by microbial growth in a plant. Adverse effects of microbial growth in plants include any type of plant tissue damage or necrosis, any type of plant yield reduction, any reduction in the value of a crop plant product, and/or the production of undesirable microbial metabolites or microbial growth byproducts (including mycotoxins). As used herein, the phrase "inhibition of microbial growth" and the like, unless otherwise indicated, may include inhibition in plants, humans, or animals.

As used herein, the phrase "percent identity" or "sequence identity" refers to the number of identical elements (i.e., amino acids or nucleotides) within a defined length of two DNA, RNA segments that produce the largest number of identical elements in an alignment, and is calculated by dividing the number of identical elements by the total number of elements within the defined length of the aligned segments and multiplying by 100.

The phrase "transgene" refers to an organism or progeny thereof, wherein the DNA of the nuclear or organelle genome of the organism or progeny organism contains an inserted exogenous DNA molecule of 10 or more nucleotides in length. The phrase "transgenic plant" refers to a plant or progeny thereof, wherein the DNA of the nuclear or plastid genome of the plant or progeny plant contains an introduced exogenous DNA molecule of 10 or more nucleotides in length. Such introduced exogenous DNA molecules may be naturally occurring, non-naturally occurring (e.g., synthetic and/or chimeric), from heterologous sources, or from autologous sources.

To the extent that any of the foregoing definitions are inconsistent with the definitions provided in any patent or non-patent reference incorporated by reference herein, any patent or non-patent reference cited herein, or any patent or non-patent reference found elsewhere, it should be understood that the foregoing definitions will be used herein.

Further description of the invention

Antimicrobial peptides and proteins referred to as defensin peptide variants are provided herein. These antimicrobial peptides and proteins can be directly applied to plants, feeds or foods; the application to the plant may be in the form of a microorganism producing the defensin peptide variant or protein, or the plant may be genetically edited to produce the defensin peptide variant or protein. The disclosure also relates to recombinant or edited polynucleotides, microorganisms and plants transformed with the recombinant or edited polynucleotides, plants comprising a genetically edited nuclear or plastid genome encoding defensin peptide variants and proteins, and compositions comprising defensin peptide variants and proteins useful for controlling pathogenic microorganisms including plant pathogenic microorganisms. In certain embodiments, a defensin variant protein comprising two defensin peptide variants or one defensin peptide variant and another peptide (including a defensin peptide variant or a defensin peptide) may provide improved microbial growth inhibition when compared to a protein comprising only one of the antimicrobial peptides found in the defensin variant protein. In certain embodiments, the defensin peptide variants and proteins provided herein are cation tolerant. Such cation tolerant defensins may be more effective than cation sensitive defensins in providing effective control of phytopathogenic microorganisms in transgenic crops. The cation-tolerant defensins provided herein can function in the normal cation-rich physiological environment of plant tissue (e.g., inhibit plant pathogenic microorganisms including fungal pathogens). The cation-tolerant defensins provided herein can also function in the normal cation-rich physiological environment of a subject (e.g., human or animal) infected with a pathogenic microorganism (e.g., inhibit pathogenic microorganisms including fungal pathogens).

Provided herein are recombinant polynucleotides comprising a polynucleotide encoding a first antifungal peptide operably linked to a polynucleotide comprising a promoter heterologous to the polynucleotide encoding the first antifungal protein. In certain embodiments, the first antifungal peptide is a defensin peptide variant.

Defensin peptide variants include a peptide ：Xaa1-G-Xaa3-C-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Xaa10-Xaa11-Xaa12-Xaa13-Xaa14-Xaa15-Xaa16-Xaa17-Xaa18-Xaa19-Xaa20-Xaa21-Xaa22-Xaa23, comprising, consisting essentially of, or consisting of the following sequence wherein Xaa1 is F, G or absent; xaa3 is A, G, Y, H, S, R or Dab; xaa5 is H, L, S, N, R, K or Dab; xaa6, xaa7, xaa8, xaa9, xaa10, xaa11, xaa12, xaa13, xaa14, and Xaa15 are each independently R, K, H, N, D, F, G, Q, P, A, I, L, V, Y, S or Dab, wherein one or more of Xaa8, xaa9, xaa10, xaa11, xaa12, xaa13, xaa14, and/or Xaa15 may be absent; xaa16 is C, V, W, Y or F; xaa17 is L, I, W, Y or F; xaa18 is C, V, W, Y or F; xaa19 is K, T, F, W or Y; xaa20 is R, K, T, L, F, Q or Dab, xaa21 is N, H, K, R, P, Q, I, F, W or Y; xaa22 is N, H, K, P, Q, I, F, W, Y, or absent; and Xaa23 is C and optionally amidated, wherein Dab is diaminobutyric acid (SEQ ID NO: 1). In certain embodiments, the peptide comprising SEQ ID NO. 1 may further comprise one or more additional amino acids located at the C-terminus of SEQ ID NO. 1, wherein the C-terminal amino acid is optionally amidated. In certain embodiments, the peptide comprising SEQ ID NO. 1 may comprise modified gamma core variant sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-8(F/W/Y)(SEQ ID NO:43) or GXCX3-9 (F/W/Y) (SEQ ID NO. 44). Defensin peptide variants also include peptides encompassed by the following sequences: xaa1-GRC-Xaa5-GFRRR (F/W/Y) (F/W/Y) (F/W/Y) XXXC- (NH ₂) wherein Xaa1 is F, G or absent; xaa5 is R or K; xaa14 is T, F, W or Y; xaa15 is R, K or Dab; xaa16 is I, F, W or Y; and Xaa17 is C and optionally amidated, wherein Dab is diaminobutyric acid (SEQ ID NO: 14). Defensin peptide variants also include peptides ：Xaa1-G-Xaa2-C-Xaa5-Xaa6-Xaa7-GF-Xaa10-Xaa11-Xaa12-(F/W/Y)(F/W/Y)(F/W/Y)-Xa a16-Xaa17-Xaa18-C-(NH₂), encompassed by the sequence wherein Xaa1 is F, G or absent and Xaa3 is A, F, W, Y or absent; xaa5 is H, R or K; xaa6 is R or K; xaa7 is Q, R or K; xaa10 is G, R or K; xaa11 is F, W, Y, R or K; xaa12 is A, F, W, Y, R or K; xaa16 is F, W, Y, R or K; xaa17 is R or K; xaa18 is R or K; and the C-terminal cysteine is optionally amidated (SEQ ID NO: 17). The defensin variant peptides set forth herein do not comprise the corresponding full-length sequences of the wild-type defensin peptides of SEQ ID NOs 8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41. Table 1 sets forth the defensin peptide variants of SEQ ID NO. 1, 3-7, 11, 13 and 14, as well as the GMA4C peptides of SEQ ID NO. 2 and SEQ ID NO. 8. In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 30 amino acid residues.

TABLE 1 defensin peptide and defensin peptide variants

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¹ Substitution of the cysteines at Xaa11 and Xaa13 with valine (GMA4C_V3A; SEQ ID NO: 5) or with tryptophan (GMA4C_V6; SEQ ID NO: 13) may provide a cyclic peptide having a disulfide bond between residue 4 cysteine and residue 17 cysteine.

² Dab = diaminobutyric acid

³ Amidation state: -NH ₂ is a C-terminal amidated peptide, and- (NH 2) is an optionally amidated C-terminal peptide

In certain embodiments, the defensin peptide variants of the disclosure are characterized as containing a defensin gamma core peptide involved in antifungal activity of a plant defensin. Gamma core peptides typically contain a net positive charge and have at least one hydrophobic amino acid. In certain embodiments, the defensin peptide variant may comprise a gamma core consensus sequence of GXCX-9C, wherein X is any amino acid (SEQ ID NO: 9). In certain embodiments, the defensin peptide variant comprises a gamma core peptide having a variant gamma core consensus sequence of GXCX3-10C, wherein X is any amino acid (SEQ ID NO: 10). In certain embodiments, the defensin peptide variant comprises a gamma core consensus sequence of GXCX-9C (SEQ ID NO: 9) or GXCX3-10C (SEQ ID NO: 10); wherein X is preferably selected from cationic amino acids and/or hydrophobic amino acids. In certain embodiments, the defensin peptide variant comprises SEQ ID NO. 1, a gamma core consensus sequence comprising GXCX C (SEQ ID NO. 9) or GXCX C (SEQ ID NO. 10); wherein X is preferably selected from cationic amino acids and/or hydrophobic amino acids, wherein any variable (Xaa) amino acid residue of SEQ ID NO. 1 comprises an amino acid residue that maintains or increases the number of cationic amino acids and/or hydrophobic amino acids found in SEQ ID NO. 3-7. It is believed that gamma core peptides are involved in phospholipid and/or sphingolipid binding, while specific amino acids outside the gamma core motif are also involved in phospholipid and sphingolipid binding. With respect to SEQ ID NO. 1, the sequence between the first cysteine (C1) and the second cysteine (C2) from the amino terminus (or the corresponding region in the defensin peptide variant in some embodiments) also contributes to antimicrobial activity.

Defensin peptide variants comprising a variant defensin C-terminal peptide provided herein may also comprise substitution of one or more conserved cysteine residues of a wild type defensin C-terminal peptide sequence. The conserved cysteine residues that may be substituted may correspond to C1, C2, C3 or C4 in the defensin C-terminal peptide, C1 being the N-terminal most cysteine, C2 being the 2 nd N-terminal most cysteine, C3 being the 3 rd N-terminal most cysteine, C4 being the C-terminal most cysteine; (see FIG. 2). Non-limiting subsets of reference defensin C-terminal peptides comprising C1, C2, C3 and C4 conserved cysteines include the GMA4C peptide of SEQ ID NO:8, the MtDef5A peptide of SEQ ID NO:16, the MtDef5A peptide of SEQ ID NO:41, the MtDef6 peptide of SEQ ID NO:20, the OeDef peptide of SEQ ID NO:23, the OeDef peptide of SEQ ID NO:26, the SbDef peptide of sequence ID NO:29, the NaD 1C-terminal peptide (SEQ ID NO: 37), the RsAFP 2C-terminal peptide (SEQ ID NO: 38), the DmAMP 1C-terminal peptide (SEQ ID NO: 39) and HsAFP (SEQ ID NO: 40) and may be substituted at least at the C2 and/or C3 positions to obtain defensin peptide variants. Additional non-limiting reference defensin C-terminal peptides comprising C1, C2, C3 and C4 conserved cysteines include the defensin C-terminal peptides shown in SEQ ID NOS 89-122. Additional non-limiting reference defensin C-terminal peptides comprising C1, C2, C3 and C4 conserved cysteines include the defensin C-terminal peptides contained in the full-length defensin peptides of SEQ ID NOS 123-434 and 436-576.

In certain embodiments, the defensin peptide variants provided herein will lack the classical gamma core consensus sequence of GXCX C-9C (SEQ ID NO: 9) or GXCX C-10C (SEQ ID NO: 10). In certain embodiments, the defensin peptide variants provided herein will comprise substitution of tryptophan, tyrosine, or phenylalanine for the C-terminal cysteine residue of the gamma core consensus sequence of SEQ ID NOs 9 or 10 (e.g., with reference to C2 in SEQ ID NOs 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, or 89 to 122). In certain embodiments, the defensin peptide variants provided herein comprise substitutions of C2 and C3 residues corresponding to C2 and C3 residues of a reference C-terminal defensin peptide (e.g., SEQ ID NO:8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41 or 89 to 122). Valine-substituted GMA4C variants with C2 and C3 are shown in SEQ ID NO. 5. A variant of GMA4C with tryptophan substitutions of C2 and C3 is shown in SEQ ID NO. 13. In certain embodiments, the defensin peptide variants provided herein comprise tryptophan, tyrosine, or phenylalanine substitutions of C2 and C3 residues corresponding to C2 and C3 residues of a reference C-terminal defensin peptide (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, or 89 to 122). In certain embodiments, the defensin peptide variants set forth herein may also comprise a tryptophan, tyrosine, or phenylalanine residue at an amino acid position between C2 and C3 residues in a reference C-terminal defensin peptide (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, 89 to 122). The tryptophan, tyrosine or phenylalanine substituted defensin peptide variants comprising C2 and C3 residues corresponding to C2 and C3 residues in the reference C-terminal defensin peptide set forth herein may comprise a modified gamma core variant sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(SEQ ID NO:43) or GXCX3-9 (F/W/Y) (SEQ ID NO: 44). In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 30 amino acid residues. In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: peptides corresponding to the C-terminal ends of defensin peptides comprising C1, C2, C3 and C4 conserved cysteines (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, 89-122 or contained in full length defensin peptides of SEQ ID NOs: 123 to 434 and 436 to 576), wherein tryptophan, tyrosine or phenylalanine substitutions corresponding to C2 and C3 residues of C2 and C3 residues in a reference C-terminal defensin peptide may result in a defensin variant peptide comprising: modified gamma core variant sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(SEQ ID NO:43) or GXCX-9 (F/W/Y) (SEQ ID NO: 44), and optionally comprising a peptide having only two (2) cysteine residues corresponding to the conserved C1 and C4 cysteines of the reference defensin C-terminal peptide.

In certain embodiments, additional defensin peptide variants are provided that lack the classical gamma core consensus sequence of GXCX C-9C (SEQ ID NO: 9) or GXCX C-10C (SEQ ID NO: 10). In certain embodiments, the defensin peptide variants provided herein will comprise substitution of tryptophan, tyrosine, phenylalanine, leucine, isoleucine, valine or methionine for the C-terminal cysteine residue of the gamma core consensus sequence of SEQ ID NO:9 or 10 (e.g., with reference to C2 in SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41 or 89 to 122, or the full length defensin peptide of SEQ ID NOs: 123 to 434 and 436 to 576). In certain embodiments, the defensin peptide variants provided herein comprise independent substitution of C2 and C3 residues with tryptophan, tyrosine, phenylalanine, leucine, isoleucine, valine or methionine residues for C2 and C3 residues corresponding to a reference C-terminal defensin peptide (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41 or 89 to 122 or contained within a full length defensin peptide of SEQ ID NOs: 123 to 434 and 436 to 576). In certain embodiments, the defensin peptide variants provided herein comprise substitution of a C2 and C3 residue with a tryptophan, tyrosine, phenylalanine, leucine, isoleucine, valine or methionine residue corresponding to the C2 and C3 residues in a reference C-terminal defensin peptide (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41 or 89 to 122 or contained within the full length defensin peptide of SEQ ID NOs: 123 to 434 and 436 to 576). In certain embodiments, the defensin peptide variants set forth herein may also comprise tryptophan, tyrosine, phenylalanine, leucine, isoleucine, valine, or methionine residues at amino acid positions located between C2 and C3 residues in a reference C-terminal defensin peptide (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, 89 to 122 or contained within a full length defensin peptide of SEQ ID NOs: 123 to 434 and 436 to 576). The defensin peptide variants set forth herein comprising independent tryptophan, tyrosine, phenylalanine, leucine, isoleucine, valine, or methionine substitutions of C2 and C3 residues corresponding to C2 and C3 residues in a reference C-terminal defensin peptide can produce a defensin variant peptide comprising: modified gamma core variant sequences GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51) or GXCX-10 (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (SEQ ID NO: 52). In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 30 amino acid residues. In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: a C-terminal peptide corresponding to a defensin peptide comprising C1, C2, C3 and C4 conserved cysteines (e.g., SEQ ID NOs: 8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, 89-122 or comprised in a full length defensin peptide of SEQ ID NOs: 123 to 434 and 436 to 576), wherein independent tryptophan, tyrosine, phenylalanine, leucine, valine, isoleucine or methionine substitutions of C2 and C3 residues corresponding to C2 and C3 residues in the reference C-terminal defensin peptide can result in a defensin variant peptide comprising a modified gamma core variant sequence of SEQ ID NO:48, 49, 50, 51 or 52 and optionally comprising a peptide having only two (2) cysteine residues corresponding to the conserved C1 and C4 cysteines of the reference defensin C-terminal peptide.

In certain embodiments, the defensin peptide variant comprises an amino acid sequence having at least 70%, 75%, 80%, 82%, 85%, 90%, 92%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NO:3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585, wherein the peptide does not comprise the corresponding full length or C-terminal sequence of the defensin peptide of SEQ ID NO:8, 16, 20, 23, 26, 29, 37, 38, 39, 40, 41, 123 to 434, or 436 to 576. In certain embodiments, such defensin peptide variants having at least 70%, 75%, 80%, 82%, 85%, 90%, 92%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NO:3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585 will further comprise a gamma core consensus sequence of GXCX-9C (SEQ ID NO: 9) or GXCX-10C (SEQ ID NO: 10); wherein X is any amino acid or wherein X is selected from cationic amino acids and/or hydrophobic amino acids. In certain embodiments, such defensin peptide variants having at least 70%, 75%, 80%, 82%, 85%, 90%, 92%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NO:3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585 will further comprise a modified gamma core variant sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、 or GXCX3-10 (F/W/Y/L/V/I/M) (F/W/Y/L/V/I/M) (SEQ ID NO: 52). In certain embodiments, such defensin peptide variants will have at least 70%, 75%, 80%, 82%, 85%, 90%, 92%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NOs 3-7, 2-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585, have a net positive charge at neutral pH of at least about 5.8 and/or a percent hydrophobicity of at least about 15%. In certain embodiments, the first structural feature of the defensin peptide variant is a net positive charge at neutral pH. In certain embodiments, the defensin peptide variant will have a net positive charge of at least +2, +3, +3.5, +4, +5, +6, +7, +8, +9, or +10 at neutral pH. In certain embodiments, the defensin peptide variant will have a net positive charge of at least +3, +3.5, +4, +5, +6 or +7 to about +8, +9 or +10 at neutral pH. In certain embodiments, such defensin peptide variants have a percent hydrophobicity of at least about 15% to 30%, about 16% to 19%, or about 28% to 30%. In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 30 amino acid residues. In certain embodiments, any of the foregoing defensin peptide variants comprises a peptide having only two (2) cysteine residues and optionally two cysteine residues corresponding to conserved C1 and C4 cysteines of a reference defensin C-terminal peptide.

In certain embodiments, the defensin peptide variants comprise amino acid substitutions which increase or maintain the net positive charge of the peptide at neutral pH and/or increase or maintain the hydrophobicity of the peptide. Amino acid substitutions in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 that maintain the net positive charge of the peptide at neutral pH include substitution of a lysine, arginine, or Dab (diaminobutyric acid) residue in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 with a different amino acid residue selected from the group consisting of: lysine, arginine, dab (diaminobutyric acid) or other non-naturally occurring amino acids that are positively charged at neutral pH. Amino acid substitutions in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 that increase the net positive charge at neutral pH include substitutions of polar (e.g., cysteine or threonine) or non-polar (e.g., glycine) residues in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 with different amino acid residues selected from the group consisting of: lysine, arginine, dab (diaminobutyric acid) or other non-naturally occurring amino acid residues that are positively charged at neutral pH. Amino acid substitutions in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 that maintain hydrophobicity of the peptide include substitutions of glycine, valine, phenylalanine, or isoleucine residues in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 with different amino acid residues selected from the group consisting of: glycine, alanine, valine, leucine, phenylalanine, isoleucine or methionine. Amino acid substitutions in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 that increase the hydrophobicity of the peptide include substitutions of polar (e.g., cysteine or threonine) residues in SEQ ID NO 3-7, 12-15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584, or 585 with different amino acid residues selected from the group consisting of: glycine, alanine, valine, leucine, phenylalanine or isoleucine. In certain embodiments, such substitutions that increase or maintain the net positive charge or hydrophobicity of the peptide will comprise defensin peptide variants having a gamma core consensus sequence of GXCX-9C (SEQ ID NO: 9) or GXCX-10C (SEQ ID NO: 10). In certain embodiments, such substitutions that increase or maintain the net positive charge or hydrophobicity of the peptide will comprise defensin peptide variants having modified gamma core variant sequence GXCX-8 (F/W/Y) (F/W/Y) (F/W/Y) (SEQ ID NO: 33) or GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-8(F/W/Y)(SEQ ID NO:43) or GXCX3-9 (F/W/Y) (SEQ ID NO: 44). In certain embodiments, such substitutions that increase or maintain the net positive charge or hydrophobicity of the peptide will comprise a defensin peptide variant having a modified gamma core variant sequence which may comprise a modified gamma core variant sequence of SEQ ID No. 45, SEQ ID No. 46, SEQ ID No. 47, SEQ ID No. 48, SEQ ID No. 49, SEQ ID No. 50, SEQ ID No. 51 or SEQ ID No. 52. In certain embodiments, the foregoing defensin peptide variants comprise, consist essentially of, or consist of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 22, 24, 16, 28 or 30 amino acid residues. In certain embodiments, any of the foregoing defensin peptide variants comprises a peptide having only two (2) cysteine residues and optionally two cysteine residues corresponding to conserved C1 and C4 cysteines of a reference defensin C-terminal peptide.

In certain embodiments, one or more amino acids in any of the foregoing or other variant defensin peptide variant sequences are substituted with another amino acid having a charge and polarity similar to that of the original amino acid, i.e., a conservative amino acid substitution. The defensin peptide variant or protein, or substitution of an amino acid within the defensin peptide sequence, may be selected from other members of the class to which the originally present amino acid belongs. Amino acids can be divided into the following four groups: (1) an acidic amino acid; (2) a basic amino acid; (3) neutral polar amino acids; and (4) neutral nonpolar amino acids. Representative amino acids in these different groups include: (1) Acidic (anionic, negatively charged) amino acids, such as aspartic acid and glutamic acid; (2) Basic (cationic, positively charged) amino acids such as arginine, histidine and lysine; (3) Neutral polar amino acids such as glycine, serine, threonine, cysteine, cystine, tyrosine, asparagine, and glutamine; (4) Neutral nonpolar (hydrophobic) amino acids such as alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan and methionine. Conservative amino acid changes within a defensin peptide sequence may be made by substituting one amino acid within one of these groups with another amino acid within the same group. The biological functional equivalent of the defensin peptide variant may have 10 or fewer conservative amino acid changes, 7 or fewer conservative amino acid changes, or 5, 4, 3, 2, or 1 conservative amino acid changes. Thus, the coding nucleotide sequence (e.g., gene, plasmid DNA, cDNA or synthetic DNA) will have the corresponding base substitution that enables it to encode a biologically functional equivalent form of the defensin peptide variant. Also provided are certain semi-conservative substitutions in defensin peptide variants comprising: (i) Substitution of neutral polar amino acid residues with neutral non-polar (hydrophobic) amino acid residues; or (ii) a neutral non-polar (hydrophobic) amino acid residue is substituted with a neutral polar amino acid residue. In particular, semi-conservative substitution of a neutral polar tyrosine residue by a hydrophobic amino acid residue is provided. The biological functional equivalent of the defensin peptide variant may have 10 or less semi-conservative amino acid changes, 7 or less semi-conservative amino acid changes, or 5, 4, 3, 2, or 1 semi-conservative amino acid changes.

Also provided herein are nucleic acid molecules encoding any of the foregoing defensin peptide variants. Also provided herein are recombinant DNA molecules comprising the foregoing nucleic acid molecules, and in particular, recombinant DNA molecules comprising a heterologous promoter operably linked to the foregoing nucleic acid molecules.

The defensin peptide variants provided herein may be operably linked to another defensin peptide variant, defensin or antimicrobial peptide by a spacer peptide sequence which is not readily cleavable by an endoprotease (including a plant endoprotease). Such peptide linker sequences have been disclosed (Argos, 1990;George RA,Heringa (2002)) that link peptides in multimeric or multidomain proteins. Examples of suitable peptide sequences from multimeric or multidomain proteins that may be used as spacer domains include immunoglobulin hinge regions from immunoglobulins, linkers between the lipoyl and E3 binding domains in pyruvate dehydrogenase (Turner et al, 1993), linkers between the central domain and the C-terminal domain of cysteine proteases (P9; mottram et al, 1989), and functional variants thereof. The spacer peptide for use in the defensin variant protein may also be a fully or partially synthetic peptide sequence. Such synthetic spacer peptides are designed to provide flexible linkage between at least one defensin peptide variant and another peptide (including a defensin peptide variant or a defensin peptide) and to resist cleavage by endogenous plant or other endoproteases. In certain embodiments, the synthetic spacer peptide may be between about 3, 4, 8, 10, 12, or 16 to about 20, 24, 28, 30, 40, or 50 amino acid residues in length. In certain embodiments, the synthetic spacer peptide may comprise a glycine-rich or glycine/serine-containing peptide sequence. The composition and design of peptides described in the literature (Chen et al, 2013) that are suitable for flexible linkage of protein domains can be tailored for use as spacer peptides in the defensin variant proteins provided herein. The spacer peptides described in U.S. patent application publications US20190194268 and US20190185877 (each of which is incorporated herein by reference in its entirety) that can be used to link defensin monomers can also be used to link the defensin peptide variants disclosed herein with other defensin peptide variants, defensins, antimicrobial peptides or other peptides.

The defensin peptide variants provided herein can be operably linked to another defensin peptide variant, defensin or antimicrobial peptide by a linker peptide sequence susceptible to cleavage by an endoprotease, including a plant endoprotease. In certain embodiments, the resulting defensin variant proteins can be expressed in a cell such that the endoprotease cleaves the defensin variant protein to provide at least one defensin peptide variant and another peptide (including a defensin peptide variant or a defensin peptide). Such defensin variant proteins may be provided in a cellular compartment (e.g. cytoplasm, mitochondria, plastid, vacuole or endoplasmic reticulum) or extracellular space (i.e. apoplast) with an endoprotease that cleaves the linker peptide. Cleavable linker peptides are disclosed in W02014078900, VASIVARAMA and Kirti,2013a, frankois et al, VASIVARAMA and Kirti,2013b, and WO 2017127558, and can be used in the defensin variant proteins provided herein.

Expression cassettes can be constructed that provide for expression of defensin peptide variants in monocots, dicots, or both. Such defensin peptide variant expression cassette construction may be effected in a plant expression vector or in a plant genome. The expression cassette is a DNA construct in which various promoters, coding (e.g., defensin peptide variant coding) and polyadenylation sequences are operably linked. In general, expression cassettes typically comprise a promoter operably linked to a sequence of interest, which promoter is operably linked to a polyadenylation or terminator region. The inclusion of intron sequences may also be useful in certain circumstances, including expression of recombinant or edited polynucleotides in monocots. When an intron sequence is included, it is typically placed in the 5' untranslated leader region of the recombinant or edited polynucleotide. In some cases, it may also be useful to incorporate specific 5' untranslated sequences into recombinant or edited polynucleotides to enhance transcript stability or promote efficient translation of the transcript. Expression cassettes and vectors for expression of other defensin peptides or proteins in plants (including those disclosed in U.S. patent No. 10253328, incorporated herein by reference in its entirety) may be adapted to express defensin peptide variants in transgenic plants. Any defensin peptide variant expression vector can be introduced into the chromosome of a host plant by methods such as: agrobacterium-mediated transformation, rhizobium-mediated transformation, sinorhizobium-mediated transformation, particle-mediated transformation, DNA transfection, DNA electroporation, or "whisker" mediated transformation. The foregoing methods of introducing transgenes are described in U.S. patent application publication No. 20050289673 (agrobacterium-mediated corn transformation), U.S. patent No. 7,002,058 (agrobacterium-mediated soybean transformation), U.S. patent No. 6,365,807 (particle-mediated rice transformation), and U.S. patent No. 5,004,863 (agrobacterium-mediated cotton transformation), each of which is incorporated herein by reference in its entirety.

In certain embodiments, plants comprising recombinant or edited polynucleotides encoding defensin peptide variants can be obtained by using techniques that provide site-specific insertion of heterologous DNA into the plant genome (e.g., by CRISPR, TALEN, or zinc finger nuclease-mediated gene editing). In certain embodiments, the DNA fragment encoding at least the defensin peptide variant is site-specifically integrated into the genome of a plant cell, tissue, part or whole plant to produce within the genome a sequence encoding the defensin peptide variant. Examples of methods for inserting exogenous DNA into specific sites in a plant genome using site-specific nucleases (e.g., meganucleases or zinc finger nucleases) are disclosed in at least Voytas,2013. Examples of methods for inserting exogenous DNA into a plant genome using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) -associated (Cas) guide RNA technology and Cas endonucleases are disclosed at least in the following: SVITASHEV et al, 2015; murovec et al, 2017; kumar and Jain,2015; and U.S. patent application publication number 20150082478, which is specifically incorporated herein by reference in its entirety.

Expression of defensin peptide variants in yeast is also specifically contemplated herein. The construction of expression vectors for the production of heterologous proteins in various Saccharomyces is well established. In general, such expression vectors typically comprise a promoter operably linked to a sequence of interest, which promoter is operably linked to a polyadenylation or terminator region. Examples of yeasts that have been used to successfully express heterologous genes include candida, kluyveromyces, hansenula (Hansuela), pichia, saccharomyces, schizosaccharomyces, and yarrowia. Kingsman et al (1985) describe generally Saccharomyces expression vectors and transformation systems. Expression vectors and transformation systems useful for yeasts other than Saccharomyces are described in Reiser et al (1990). Expression cassettes and vectors for expression of other defensin peptides or proteins in yeast (including those disclosed in U.S. patent No. 10253328, incorporated herein by reference in its entirety) may be adapted for expression of defensin peptide variants in yeast.

The expression of defensin peptide variants in bacteria is also specifically contemplated herein. Construction of expression vectors for the production of heterologous proteins in various bacterial systems has been described. In general, such expression vectors typically comprise a promoter operably linked to a sequence encoding one or more peptide sequences of interest (e.g., the n-terminal signal transit peptide region of a defensin variant peptide), which promoter is operably linked to a prokaryotic terminator region. Examples of the genus bacteria that have been used for successful expression of heterologous genes include Acinetobacter, alcaligenes, azotobacter, bacillus, escherichia, lactobacillus, lactococcus, streptomyces and Pseudomonas. Coli expression systems useful for producing proteins comprising disulfide bonds and which may be adapted for expression of the defensin variant peptides provided herein include those described in the following: kuddus et al (2017) Biotechnol Prog [ Biotechnology progress ]233:1520-1528.Doi:10.1002/btpr.protein Science [ protein Science ]2508; kiedzierska et al (2008) Protein Expr Purif [ protein expression and purification ]60,82-88; chang et al (2015) Amino Acids [ Amino Acids ]47,579-587; buchko et al (2018) Protein Science [ Protein Science ]27,1611-1623; marques et al (2008) J Appl Microbiol [ journal of applied microbiology ]106,1640-1648; and Pazgier et al (2006) Protein Expr Pur [ Protein expression and purification ]49,1-8. The system for expressing disulfide bond containing proteins may be adapted for expression of defensin peptides in E.coli, including those disclosed in: U.S. patent publication No. US2020/0172915 (which is incorporated herein by reference in its entirety), berkmen, m.protein Expr Purif, [ protein expression and purification ]2012;82 (1) 240-51.Doi:10.1016/j. Pep.2011.10.009.

Other bacterial expression systems that can be used to produce proteins comprising disulfide bonds and that can be adapted for expression of the defensin variant peptides provided herein include those described in the following: U.S. patent No. 10,604,761 (which is incorporated herein by reference in its entirety).

Also provided are antimicrobial compositions for agricultural, pharmaceutical or veterinary use comprising an antimicrobial plant or antimicrobial human or veterinary pathogenic microorganism inhibiting amount ("antimicrobial effective amount") of one or more of the isolated, purified antimicrobial defensin peptide variants of the invention, or a biofunctional equivalent thereof. Such compositions may comprise one or any combination of the defensin peptide variants or proteins disclosed herein, together with an agriculturally, pharmaceutically or veterinarily practically acceptable carrier, diluent or excipient. Other components relevant in agricultural and therapeutic contexts may also be included in such compositions, as shown below. The antimicrobial compositions are useful for inhibiting the growth of or killing defensin peptide variants susceptible to pathogenic microorganisms associated with microbial infection of plants, humans or animals. Such antimicrobial compositions may be formulated for topical application and applied topically to plants, plant environments (including soil), or humans or animals. Such antimicrobial compositions can be formulated for enteral, parenteral, and/or intravenous administration of the composition, and can be administered to a subject in need thereof; such subjects may be humans, livestock, poultry, fish, or companion animals. The defensin peptide variants may be formulated alone or in any combination with one another, and any of these may additionally be formulated in combination with other conventional antimicrobial therapeutic compounds as follows: for example, as a non-limiting example, a polyene antimicrobial agent; imidazole, triazole, and thiazole antimicrobial agents; allyl amine; and echinocandins commonly used in human and veterinary medicine. Compositions comprising defensin peptide variants may be administered to a human or animal subject in need thereof by a variety of routes including topical, enteral, parenteral and/or intravenous administration. Antimicrobial peptides and compositions are useful for controlling microbial pathogens or contaminants, including: (i) A bacterial pathogen of a plant or animal, wherein the bacterial pathogen is optionally a member of the family enterobacteriaceae, and optionally wherein the bacterial pathogen is a salmonella species, an escherichia species, or a listeria species; (ii) Is Fusarium species, alternaria species, rhizopus species, verticillium species, phytophthora species, cephalosporium species, botrytis species, cercospora species, phytophthora species, rhizoctonia species, sclerotinia species, myrothecium species, phoma species, phytophthora species, torulopsis species, and Tarulopsis species Puccinia species, septoria species, penicillium species, maackia species, phomopsis species, sphaerotheca species, alternaria species, erysiphe species, leptosphaeria species, or Leptosphaeria species; (iii) Aspergillus, cryptococcus, penicillium, rhizopus, leptosphaeria, saccharomyces, rhizomucor, cohead, colocasia, mucor, pythium, fusarium, histoplasma, coccidioides or Agrimonia species; (iv) A Candida species, and wherein the Candida species is Candida albicans (Candida albicans/c.albicans), candida otophylla, candida glabrata, candida parapsilosis, candida tropicalis, or Candida krusei; or (v) dermatophytes optionally selected from the group consisting of: trichophyton rubrum, trichophyton digitatum, trichophyton purple, trichophyton aphanidermatum, trichophyton sudan, trichophyton mentagrophytes, microsporum flavum (Microsporum flavum), epidermophyton floccosum and Microsporum gypseum.

Agricultural compositions comprising any of the defensin peptide variant molecules of the invention, alone or in any combination, may be formulated as described in the following: such as Winnacker-Kuchler (1986) Chemical Technology [ chemical technology ], fourth edition, volume 7, HANSER VERLAG, munich [ Munich Hansa Press ]; van Falkenberg (1972-1973) PESTICIDE FORMULATIONS [ pesticide ], second edition, MARCEL DEKKER, N.Y [ new york makert de ke press ]; and K.Martens (1979) SPRAY DRYING Handbook [ Handbook of spray drying ], third edition, G.Goodwin, ltd., london's Drum temperature group, inc. Formulation aids (e.g., carriers, inert materials, surfactants, solvents, and other additives) are also well known in the art and are described, for example, in the following: watkins, handbook of Insecticide Dust Diluents AND CARRIERS [ handbook of pesticide dust diluents and carriers ], second edition, darland Books, caldwell, N.J. (Kandeowel Darland Books Press, N.Y. and Winnacker-Kuchler (1986) Chemical Technology [ chemical technology ], fourth edition, volume 7, HANSER VERLAG, munich [ Munich Hansa Press ]. Using these formulations, it is also possible to prepare mixtures of the defensin peptide variants and proteins of the invention with other pesticidally active substances, fertilizers and/or growth regulators and the like, in the form of final formulations or tank mixtures.

The antimicrobial defensin peptide variants of the invention can be administered to a subject or plant at a pH ranging from about 3.0 to about 9.0 at a concentration ranging from about 0.1pg/ml to about 100mg/ml, or from about 5pg/ml to about 5mg/ml, either alone or in combination with other active agents. Such compositions may be buffered using, for example, about 1mM to 1M, about 10mM to about 100mM, or about 15mM to about 50mM phosphate buffer. In the case of low buffer concentration, salts may be added to increase the ionic strength. In certain embodiments, sodium salts (including NaCl) in the range of about 1mM to about 1M, about 1mM, 5mM, or 10mM to about 20mM, 50mM, 100mM, 150mM, or 200mM, or about 10mM to about 100mM may be added or provided in a composition comprising a defensin peptide variant and a protein. In certain embodiments, potassium salts (including KC 1) in the range of about 1mM, 5mM, or 10mM to about 20mM, 50mM, 100mM, 150mM, or 200mM can be added or provided in a composition comprising a defensin peptide variant and a protein. In certain embodiments, a calcium salt (including CaCl ₂) in the range of about 0.1mM, 0.5mM, or 1mM to about 2mM, 5mM, 10mM, or 20mM may be added or provided in a composition comprising the defensin peptide variant.

Many conventional microbial antibiotics and chemical antimicrobial agents (e.g., fungicides) that can be combined with the defensin peptide variants and proteins of the invention are described in Worthington and Walker (1983) THE PESTICIDE Manual, seventh edition, british Crop Protection Council, british crop protection committee. These include, for example, polyoxin, solar mycin (nikkomycin), carboxamides, aromatic carbohydrates, carboxin (carboxine), morpholines, sterol biosynthesis inhibitors, and organophosphorus compounds. In addition, azole, triazole and echinocandin fungicides can be used. Other active ingredients that may be formulated in combination with the antimicrobial peptides and proteins of the invention include, for example, insecticides, attractants, bactericides, acaricides, nematicides and herbicides. U.S. patent No. 5,421,839, which is incorporated herein by reference in its entirety, contains a comprehensive summary of many active agents that can be formulated with substances such as the antimicrobial defensin peptide variants and proteins of the invention.

Agriculturally useful antimicrobial compositions contemplated herein also include those in the form of host cells (e.g., bacterial and microbial cells) that are capable of producing defensin peptide variants and proteins and which can be colonized by plants, including roots, shoots, leaves, or other parts of plants. The term "plant colonizing microorganism" is used herein to refer to a microorganism capable of colonizing any part of the plant itself and/or the plant environment, including and capable of expressing the defensin variant antimicrobial peptides and proteins of the invention in the plant and/or the plant environment. A plant colonizing microorganism is a microorganism that may be present in symbiotic or harmless relation to plants in a plant environment. U.S. patent No. 5,229,112, which is incorporated herein by reference in its entirety, discloses a variety of plant colonizing microorganisms that can be engineered to express antimicrobial peptides and proteins, and methods of use thereof, suitable for use with the defensin variant antimicrobial peptides and proteins disclosed herein. Plant colonizing microorganisms expressing the defensin variant antimicrobial peptides and proteins disclosed herein useful for inhibiting microbial growth in plants include bacteria selected from the group consisting of: bacillus species including Bacillus thuringiensis, bacillus israeli and Bacillus subtilis, and Bacillus phloem Asian species (Candidatus Liberibacter asiaticus); pseudomonas species; a Arthrobacter species, a azospirillum (Azospyrillum) species, a rod-like bacillus species, and an escherichia species; agrobacterium species, such as Agrobacterium radiobacter (A.radiobacter); rhizobium species, erwinia species, azotobacter species, azospirillum (Azospirillum) species, klebsiella species, alcaligenes species, rhizosphere bacillus species, xanthomonas species, rogowski (Ralstonia) species, and flavobacterium species. In certain embodiments, the microorganism is a yeast selected from the group consisting of: saccharomyces cerevisiae, pichia pastoris, and Pichia methanolica. In certain embodiments, the plant colonizing microorganism may be an endophyte or a microorganism. Rhizosphere colonising bacteria from the genus pseudomonas are particularly useful when the defensin peptide variant molecules of the invention are applied to the rhizosphere, especially fluorescing pseudomonas, such as fluorescing pseudomonas (Pseudomonas fluorescens), which is particularly competitive in plant rhizosphere and mass colonising plant root surfaces. Examples of suitable foliar (leaf) colonising bacteria are pseudomonas putida, pseudomonas syringae and erwinia species.

Examples

The following numbered embodiments form part of the present disclosure:

1a. A peptide comprising the sequence SEQ ID No. 1, wherein the peptide does not comprise the corresponding full length sequence of the defensin peptide of SEQ ID No. 8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41; optionally wherein the peptide comprises the modified gamma core consensus sequence of GXCX-8 (F/W/Y) (F/W/Y) (F/W/Y) shown in SEQ ID NO:33, GXCX-9 (F/W/Y) (F/W/Y) (F/W/Y), GXCX3-8 (F/W/Y) (SEQ ID NO: 43), or GXCX3-9 (F/W/Y) (SEQ ID NO: 44) shown in SEQ ID NO: 34; and/or optionally wherein the C-terminal cysteine residue or C-terminal amino acid residue is amidated.

A peptide having at least 50%, 55%, 60%, 68%, 75%, 82% or 94% sequence identity over the full length of any one of SEQ ID NOs 3, 4, 5 or 6, wherein the peptide is not identical to SEQ ID NO 8, optionally wherein any amino acid substitution in said sequence increases or maintains the net positive charge of the peptide at neutral pH and/or increases or maintains the hydrophobicity of the peptide, and optionally wherein the C-terminal cysteine residue is amidated.

A peptide having at least 50%, 55%, 60%, 68%, 75%, 82%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NO:7, 12, 13, 14, 15, 17-20, 22, 23, 25, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585, wherein the peptide is not identical to SEQ ID NO:8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41, optionally wherein the peptide of SEQ ID NO:5, SEQ ID NO:13, 14, 15, 18, 19, 20, 22, 23, 26, 28, 29, 31, 32, 42, 578, 579, 581, 582, 584 or 585 comprises a disulfide bond between two cysteine residues, and optionally wherein any substitution increases or maintains the net positive charge of the peptide and/or increases or maintains the hydrophobicity of the peptide at neutral pH, and optionally wherein the C-terminal cysteine residue or C-terminal amino acid residue is amidated.

A defensin C-terminal peptide variant comprising conserved C1 and C4 cysteine residues corresponding to the N-terminal and C-terminal cysteines of a reference defensin C-terminal peptide wherein the conserved C2 and C3 cysteine residues of the reference defensin C-terminal peptide are independently substituted with tryptophan, tyrosine, phenylalanine, leucine, valine, isoleucine or methionine; and optionally wherein the defensin peptide variant has a net positive charge of at least 3, 3.5, 4,5 or 6 and a hydrophobic amino acid content of at least 18%.

A C-terminal defensin peptide variant comprising a modified gamma core consensus sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、 or GXCX3-10 (F/W/Y/L/V/I/M) (SEQ ID NO: 52), optionally wherein the peptide further comprises a conserved C4 cysteine residue, optionally wherein a cysteine residue in the modified gamma core consensus sequence forms a disulfide bond with the conserved C4 cysteine residue, and/or optionally wherein the defensin peptide variant has a net positive charge of at least 3, 3.5, 4, 5 or 6 and a hydrophobic amino acid content of at least 18%.

A peptide comprising a modified gamma core consensus sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-12(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:588)、GXCX3-15(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:589)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、 or GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、 or GXCX3-10 (F/W/Y/L/V/I/M) (SEQ ID NO: 52), wherein the peptide further comprises a second C-terminal cysteine residue located C-terminal to the cysteine residue in the modified gamma core consensus sequence, and wherein the peptide has a net positive charge of at least 3, 3.5, 4, 5 or 6 and a hydrophobic amino acid content of at least 18%, optionally wherein the peptide comprises a disulfide bond between the two cysteine residues.

2. The peptide of embodiment 1a, 1b, 1c, 1d, 1e or 1f, wherein the peptide comprises: (i) Amino acid sequence ：SEQ ID NO:3,SEQ ID NO:4,SEQ ID NO:5,SEQ ID NO:6,SEQ ID NO:7,SEQ ID NO:12,SEQ ID NO:13,SEQ ID NO:14,SEQ ID NO:15、17-20、22、23、25、26、28、29、31、32、42、578、579、581、582、584 or 585 selected from the group consisting of; optionally wherein the peptide of SEQ ID No. 5,SEQ ID NO:13,SEQ ID NO:14, 15, 18, 19, 20, 22, 23, 26, 28, 29, 32, 42, 578, 579, 581, 582, 584, or 585 comprises a disulfide bond between two cysteine residues; (ii) An amino acid sequence having at least 50%, 55%, 60%, 68%, 75%, 82%, 94%, 95% or 100% sequence identity over the full length of SEQ ID NO:7,SEQ ID NO:12,SEQ ID NO:13,SEQ ID NO:14、15、17-20、22、23、25、26、28、29、31、32、18、19、20、22、23、26、28、29、32、42、578、579、581、582、584 or 585, wherein the peptide does not comprise the corresponding full length sequence of the defensin peptide of SEQ ID NO 8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41; and optionally the peptide comprises a disulfide bond between two cysteine residues; or (iii) wherein the peptide exhibits antimicrobial activity.

3. The peptide of embodiment 1a, 1b, 1c, 1d, 1e, 1f, or 2, wherein the peptide comprises, consists essentially of, or consists of: (i) 30 amino acid residues or less; (ii) 15, 16 or 17 to 30 amino acid residues; or (iii) 15, 16 or 17 to 30 amino acid residues and contains two cysteine residues.

4. A composition comprising the peptide of any one of embodiments 1a, 1b, 1c, 1d, 1e or 1f to 3 and an agriculturally, pharmaceutically or veterinarily practically acceptable carrier, diluent or excipient.

5. The composition of embodiment 4, wherein the peptide is provided at a concentration of about 0.1, 0.5, 1.0, or 5pg/ml to about 1,5, 20, 50, or 100mg/ml or at a concentration of about 0.1, 0.5, 1.0, or 5 pg/g to about 1,5, 20, 50, or 100 mg/g, and optionally wherein the composition comprises a sodium salt at a concentration of at least 100mM and/or a calcium salt at a concentration of at least 2 mM.

6. A method for: (i) Preventing or reducing damage to crops by phytopathogenic microorganisms; or (ii) preventing contamination of a plant, plant part, seed, feed obtained therefrom, or food product obtained therefrom with undesirable microorganisms, comprising the step of contacting a plant, plant seed or other part of said plant, feed obtained therefrom, or food product obtained therefrom with an effective amount of a composition as described in example 4 or example 5.

7. The method of embodiment 6, wherein the plant pathogenic microorganism or undesirable microorganism is: (i) A bacterial pathogen of a plant or animal, wherein the bacterial pathogen is optionally a member of the family enterobacteriaceae, and optionally wherein the bacterial pathogen is a salmonella species, an escherichia species, or a listeria species; or (ii) is Fusarium species, alternaria species, rhizopus species, verticillium species, phytophthora species, cephalosporium species, botrytis species, cercospora species, puccinia species, rhizoctonia species, sclerotinia species, pythium species, phoma species, secoisolarium species, acremonium species, puccinia species, septoria species, penicillium species, maackia species, phomopsis species, sphaerotheca species, alternaria species, erysiphe species, leptosphaeria species, or Leptosphaeria species.

8. A medical device comprising a device and the composition of example 4 or 5, wherein the device comprises at least one surface that is topically coated and/or impregnated with the composition.

The medical device of embodiment 8, wherein the device is a stent, catheter, contact lens, condom, patch, or septum.

10. A method for treating, preventing, or inhibiting a microbial infection in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of example 4 or 5.

11. The method of embodiment 10, wherein said administering comprises topical, enteral, parenteral, and/or intravenous introduction of the composition.

12. The method of embodiment 10 or 11, wherein the subject is a human, livestock, poultry, fish, or companion animal.

13. The method of embodiment 12, wherein the microbial infection is an infection of a mucosa, eye, skin and/or nail and the composition is applied to the mucosa, eye, skin and/or nail.

14. The method of any one of embodiments 10-13, wherein the microbial infection is caused by a dermatophyte, and wherein the dermatophyte is optionally selected from the group consisting of: trichophyton rubrum, trichophyton digitatum, trichophyton purple, trichophyton aphanidermatum, trichophyton sudan, trichophyton mentagrophytes, microsporum flavum, epidermophyton floccosum and Microsporum gypseum.

15. The method of any one of embodiments 10-13, wherein the microbial infection is caused by: (i) A bacterial pathogen of an animal, wherein the bacterial pathogen is optionally a member of the family enterobacteriaceae, and optionally wherein the bacterial pathogen is a salmonella species, an escherichia species, or a listeria species; or (ii) Aspergillus, cryptococcus, penicillium, rhizopus, lepidotium, kanning Anmyces, leptomyces, rhizomucor, cohead, colymyces, mucor, fusarium, histoplasma, coccidium or Agrimonia species.

16. The method of any one of embodiments 10-13, wherein the microbial infection is caused by Candida species, and wherein the Candida species is Candida albicans (Candida albicans/c.albicans), candida otophylla, candida glabrata, candida parapsilosis, candida tropicalis, or Candida krusei.

17. The composition of embodiments 4 or 5 for use in a method of treating, preventing or inhibiting a microbial infection in a subject in need thereof.

18. The composition of embodiment 17, wherein the subject is a human, livestock, poultry, fish, or companion animal.

19. A plant part at least partially coated with a composition as described in example 4 or 5.

20. The plant part of embodiment 19, wherein the part is a seed, and the seed is optionally corn, soybean, wheat, rice, cotton, brassica species, or tomato seed.

21. The plant part of embodiment 19, wherein the plant part is a fruit, vegetable or flower.

22. A recombinant polynucleotide comprising a polynucleotide encoding the antimicrobial peptide of example 1a, 1b, 1c, 1d, 1e, 1f, 2, or 3, wherein the polynucleotide encoding a first antimicrobial peptide is operably linked to a polynucleotide comprising a promoter heterologous to the polynucleotide encoding the first antimicrobial peptide, optionally wherein any amino acid substitution in the sequence increases or maintains the net positive charge of the peptide and/or increases or maintains the hydrophobicity of the peptide.

23. The recombinant polynucleotide of embodiment 22, wherein the recombinant polynucleotide further comprises a polynucleotide encoding: (i) A transit peptide, a vacuole targeting peptide and/or an endoplasmic reticulum targeting peptide; (ii) a plastid targeting peptide; and/or (iii) a polyadenylation or transcription termination signal, wherein the polynucleotides of (i), (ii) and/or (iii) are operably linked to the polynucleotide encoding the antimicrobial peptide.

24. The recombinant polynucleotide of embodiment 22 or 23, wherein the polynucleotide encoding the first antimicrobial peptide is inserted into a heterologous nucleus or plastid genome of the cell and operably linked to an endogenous promoter located in the heterologous nucleus or plastid genome.

25. A plant core or plastid genome comprising a polynucleotide encoding the antimicrobial peptide of example 1a, 1b, 1c, 1d, 1e, 1f, 2 or 3, and wherein the polynucleotide is heterologous to the core or plastid genome, and wherein the polynucleotide is operably linked to an endogenous promoter of the core or plastid genome.

26. A cell comprising the recombinant polynucleotide of example 22 or the genome of example 25, wherein the cell is optionally a bacterial, yeast or plant cell.

27. A plant comprising the recombinant polynucleotide of example 22 or the genome of example 25.

28. A plant part that is a plant part of the plant of embodiment 26, wherein the plant part comprises the recombinant polynucleotide or genome, optionally wherein the plant part is a seed, stem, leaf, root, tuber, flower or fruit.

29. A method for producing a plant seed providing a plant resistant to infection by a plant pathogenic microorganism, the method comprising the steps of: (i) selfing or crossing a plant as described in example 22; and (ii) harvesting seed comprising the recombinant polynucleotide of the plant from the selfing or crossing, thereby producing plant seed that provides a plant that is resistant to infection by a plant pathogenic microorganism.

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Examples

Example 1

Crude peptides (gma4c—ac, gma4c_v1, gma4c_v2, and gma4c_v3) were chemically synthesized by the company Biomatik, canada, gma4c_v4, and gma4c_v5 were chemically synthesized by the company allen sciences (ALAN SCIENTIFIC inc., usa) and further purified using a linear gradient of acetonitrile/water mixture in C-18 reverse phase HPLC (agilent technologies, inc (Agilent Technologies), usa). HPLC fractions were lyophilized and resuspended in nuclease free water. The concentration of each peptide was determined using a BCA assay performed according to the manufacturer's protocol (Thermo-FISHER SCIENTIFIC, USA). Antifungal activity was determined in an assay in SFM medium. SFM medium contained K₂HPO₄(2.5mM)、MgSO₄(50μM)、CaCl₂(50μM)、FeSO₄(5μM)、CoCl₂(0.1μM)、CuSO₄(0.1μM)、Na₂MoO₄(2μM)、H₃BO₃(0.5μM)、KI(0.1μM)、ZnSO₄(0.5μM)、MnSO₄(0.1μM)、 glucose (10 g/liter), asparagine (1 g/liter), methionine (20 mg/liter), inositol (2 mg/liter), biotin (0.2 mg/liter), thiamine-HCL (1 mg/liter) and pyridoxine-HCL (0.2 mg/liter), pH 7.0.

TABLE 2 antifungal Activity of peptides

¹ The C-terminus is amidated.

TABLE 3 comparison of GMA4C wild-type and variant peptides

In vitro antifungal activity under low cation conditions: the gma4c variants gma4c_v1A, GMA4c_v3A, GMA4c_v4a and gma4c_v5a exhibited in vitro antifungal activity against botrytis, fusarium graminearum, fusarium oxysporum, and phytophthora capsici comparable to the wild-type gma4c_ac control. The gma4c variant gma4c_v2a exhibited a two-fold reduction in vitro antifungal activity against botrytis cinerea and fusarium graminearum relative to the wild-type gma4c_ac control. The gma4c_gma4c_v6a exhibited a four-fold increase in vitro antifungal activity against botrytis cinerea relative to a wild-type gma4c_ac control.

Antifungal activity in plants. When applied to isolated benthamiana leaves, gma4c_v1a and gma4c_v3a were more potent than gma4c_ac or gma4c_v2a in reducing gray mold symptoms caused by botrytis, as shown in fig. 1A and 1B. When applied to tomato leaves, gma4c_v1a is more effective than gma4c_ac in reducing disease symptoms caused by phytophthora capsici.

In vitro antifungal activity in the presence of cations. When tested for anti-botrytis antifungal activity in the presence of 100mM NaCl, 100mM KCl or 2mM CaCl ₂, all peptides, including GMA4C_AC, retained their antifungal activity in the presence of 100mM NaCl or 100mM KCl. However, gma4c_v1a and gma4c_v4a are 2 times more active than gma4c_ac. Only GMA4C_V1A and GMA4C_V4A inhibited fungal growth at 6. Mu.M in the presence of 2mM CaCl ₂, while other peptides (e.g., phytoalexinMtDef 4 or OeDef 1) were barely active at this concentration.

Table 4 GMA4C and variants thereof retain antifungal activity against Botrytis cinerea in the presence of salts.

¹ The Minimum Inhibitory Concentration (MIC) is the concentration of peptide at which a microorganism does not grow significantly relative to the growth of the microorganism in a growth medium lacking the compound, protein or peptide.

Antifungal activity against human fungal pathogens: gma4c_v1a and gma4c_v3a showed antifungal activity against candida otophylla and candida glabrata in the cation-enriched RPMI medium, but the parent MtDef4 defensin did not show activity.

TABLE 5 composition of the medium of the Rockwell Parker souvenir institute 1640 (RPMI medium; containing glutamine and phenol Red but no bicarbonate)

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Table 6.Gma4c_v1a and gma4c_v3a showed in vitro antifungal activity against candida otorhinoca and candida glabrata in RPMI medium.

¹ The Minimum Inhibitory Concentration (MIC) is the concentration of a protein or peptide at which a microorganism does not grow significantly relative to the growth of the microorganism in a growth medium lacking the compound, protein or peptide.

EXAMPLE 2 Activity of GMA4C variants against pathogenic microorganisms

Antifungal Activity assay peptides were tested using half strength potato dextrose broth and the antifungal drugs fluconazole and voriconazole were compared using RPMI assay. CLSIM27 and M38 methods were used to measure MIC. The Minimum Inhibitory Concentration (MIC) is the concentration of a compound, protein or peptide at which a microorganism does not significantly grow relative to the growth of the microorganism in a growth medium lacking the compound, protein or peptide.

All tests were performed in RPMI using 0.165M MOPS buffer. The concentration of peptide in potato dextrose broth is in the range of 0.06-2mcg/ml, the concentration of fluconazole is in the range of 0.125-64mcg/ml, and the concentration of voriconazole is in the range of 0.03-16mcg/ml. MIC was measured at 24-72 hours.

TABLE 7 Potato dextrose broth formulation

MIC values for gma4c variants. Figure 4 provides a complete table containing fluconazole and voriconazole data.

Table 9 antifungal activity of GMA4C wild type and variants thereof against plant fungal pathogens in sfm.

TABLE 10 antifungal Activity of GMA4C wild type and variants thereof in SFM+ salts against plant fungal pathogens

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Table 11 antifungal Activity of peptides against human Yeast pathogens in half strength Potato dextrose broth

¹ Full length MtDef4 protein is described in U.S. patent No. 7,825,297.

All pathogen isolates used in this assay were resistant to the antifungal drug fluconazole. Antifungal assays were performed in half strength potato dextrose broth.

TABLE 12 synergistic (or additive) effects of GMA4C_v3 or MtDef 4/fluconazole combinations

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¹ FICI is a fractional inhibitory concentration index. The method of calculation was MIC _A combination/mic+mic _B combination alone/MIC _B alone, where MIC _A combination is the MIC of combined agent a and MIC _A alone is the MIC of agent a alone. In the above table, agent A is a peptide (GMAC_V3A) and agent B is fluconazole. In the table below, agent a is the full length MtDef4 protein and agent B is fluconazole from fluconazole.

² Full length MtDef4 proteins are described in U.S. patent No. 7,825,297, which is incorporated herein by reference in its entirety.

Table 13. Antifungal activity (MIC in μm) of peptides against human nail pathogens (trichophyton species) and aspergillosis (aspergillus fumigatus).

¹ Full length MtDef4 protein is described in U.S. patent No. 7,825,297.

² Full length OeDef defensin proteins are described in WO 2020/146373.

Table 14 antifungal activity (MIC in μm) of gma4c variants against candida species and aspergillosis (aspergillus fumigatus).

EXAMPLE 3 antibacterial Activity of GMA4C_V1A against human bacterial pathogens

Salmonella typhimurium Copenhagen variant, enterotoxigenic E.coli-F4 and Listeria monocytogenes (F5244) strains were grown on LB agar plates overnight at 37 ℃. Small amounts of bacteria were scraped from the plates and added to Mueller-Hinton (MH) growth medium and grown to log phase. Cells were diluted to 1-3x10 ⁶ CFU/mL and 50ul was added to each well of a polypropylene 96 well plate. The synthetic peptide gma4c_v1a was diluted to 0.2% BSA, 0.01% acetic acid solution and added to concentrations of 0.2, 0.4, 0.80, 1.6, 3.25, 7.5, 15, 30 and 60 and 120 μm. Then, 50. Mu.l of each peptide solution was added to 50. Mu.l of bacterial cells. The plates were sealed with sealing film and incubated overnight at 37 ℃. The concentration of inhibition of bacterial growth was determined based on the OD ₆₀₀ nm value for each concentration relative to the OD ₆₀₀ nm value for MH medium alone and the OD ₆₀₀ nm value for bacterial growth in MH medium without any peptide. The Minimum Inhibitory Concentration (MIC) is the concentration of peptide at which the microorganism does not grow significantly relative to the growth of the microorganism in a growth medium lacking the peptide.

TABLE 15 antibacterial Activity of GMA4C_V1A (SEQ ID NO: 3)

EXAMPLE 4 antimicrobial Activity of defensin peptide fragments

Chemically synthesized 80% -85% pure defensin-derived crude peptides were obtained from company Biomatik canada or allen sciences. Each peptide was further purified using C-18 reverse phase HPLC (Agilent, singapore). The peptide-containing HPLC fractions were lyophilized and resuspended in nuclease-free water. The BCA assay was used to determine concentration according to the manufacturer's protocol (sammer feishier technologies) to achieve accurate quantification.

Fungal strains of Botrytis cinerea T-4, alternaria alternata, cercospora sojae and Cercospora spinosa (Colletotrichum gloeosporioides) were each grown in their respective normal growth media shown in Table 16. Fungal spores were harvested by flooding the fungal growth plate with sterile water. The spore suspension was filtered through two layers of Miracloth (Miracloth), centrifuged at 13,600rpm for 1min, washed and resuspended in low salt Synthetic Fungus Medium (SFM) (us patent No. 6,316,407). The spore suspension was adjusted to the desired spore count using a hemocytometer.

Table 16

The antifungal activity of the truncated defensin-derived peptides and variants thereof against Botrytis cinerea, alternaria, leptosporum sojae and Cephalosporium longum fungal pathogens was determined spectrophotometrically using 96-well plate assays (Sagaram et al, (2011) PLoS ONE [ public science library. Complex ]6:e18550. Doi:10.1371/journ. Fine.0018550). 45 microliter of peptide at concentrations of 0.375, 0.75, 1.5, 3, 12 μm was added to each well of a microtiter plate containing 45 μl (about 10 ⁵ botrytis cinerea spores/ml) of spore suspension. After 48h, quantitative fungal growth inhibition was determined by measuring absorbance at 595nm using a (TECAN INFINITE M Pro, diken systems Co., TECAN SYSTEMS Inc.), san Jose, calif., microplate reader. Fungal cell viability was determined using a resazurin cell viability assay (Chadha and Kale, (2015) Lett Appl Microbiol [ applied microbiology flash ]61,238-244, li et al, (2019) Mol Plant Microbe Interact [ molecular plant-microorganism interactions ]32, 1649-1664). After 48h incubation of the pathogen/peptide mixture, 10 μl of 0.1% resazurin solution was added to each well and incubated again overnight. The change in color of resazurin from blue to pink or colorless indicates the presence of viable fungal cells. The MIC value for each peptide was determined as the lowest concentration of peptide where no blue change was observed. MIC values for defensin-derived peptides and variants thereof were also determined in SFM and SFM supplemented with 100mM NaCl, 100mM or 2mM CaCl ₂, as described above.

Each defensin-derived peptide and variants thereof were used as previously described for the anti-fungal activity in the semi-plant of Botrytis cinerea using isolated N.benthamiana Nb1 She Ceding (Li et al, (2019) Mol Plant Microbe Interact [ molecular plant-microorganism interactions ]32,1649-1664; velevelli et al, (2020) Proceedings of the National Academy of Sciences [ Proc. Natl. Acad. Sci. USA ].117,16043-16054). Each peptide was tested at 1.5 μm, 3 μm and 6 μm concentrations. After incubation of each peptide/fungal spore mixture for 48h at room temperature, leaves were photographed under white light. High-resolution fluorescence images were also taken using CropReporter (PhenoVation company, netherlands, wageningen) under Ning Gen. These images depict the calculated F _V/F_M (maximum quantum yield of photosynthetic system II) values for the affected area affected by botrytis cinerea infection. The colors in the image show five different categories from category I to category V (0.000 to 0.700), depicting different degrees of tissue damage. The green color in each image represents a class V in the range of 0.600 to ≡ 0.700, depicting healthy areas of the leaf surface. Conversely, red represents a class I in the range of 0.000 to 0.160 depicting severely damaged or diseased leaf surfaces.

The primary amino acid sequence, length, net charge, and percent hydrophobic amino acids of the defensin-derived peptides are shown in table 17. These peptides are derived from phytoalexins OeDef1, mtDef4, msDef1 and MtDef5A. Amino acid substitutions are made to the wild type sequence of each peptide to increase net charge and hydrophobicity. Furthermore, disulfide bonds are introduced into specific variants, making them pseudo-cyclic. Peptides that can form a single disulfide bond and pseudocyclic peptides are shown as "+" in the "disulfide bond" column of table 17. All peptides also bear a carboxyl-terminal amide group.

Table 17. Amino acid sequence, length, net charge, and% hydrophobic amino acids of defensin-derived peptides. The presence of disulfide bonds is indicated when necessary.

The Minimum Inhibitory Concentration (MIC) values for each defensin-derived peptide and variant thereof were determined (table 18). The presence of cations is presumed to significantly impair the electrostatic interaction between positively charged defensins and negatively charged fungal membranes (Chu et al, (2013) Antimicrobial AGENTS AND Chemotherapy [ Antimicrobial and chemotherapy ] 57:4050-4052). Thus, we determined the antifungal activity of each peptide against Botrytis cinerea in SFM supplemented with 100mM NaCl, 100mM KCl or 2mM CaCl ₂ (Table 18).

TABLE 18 MIC values of defensin-derived peptides against Botrytis cinerea

¹ ND is undetermined.

MIC values of defensin-derived peptides against alternaria alternata, cercospora sojae and cercospora spinosa were also tested (table 19).

TABLE 19 in vitro antifungal Activity of antifungal peptides against Alternaria alternata, alternaria sojae and Alternaria longifolia

Peptides	SEQ ID NO	Alternaria alternata (L.) Kuntze	Leptospora sojae	Cephalosporium spinosum
					GMA4AC_WT	580	>12	>12	>12
GMA 4C_V9	581	>12	6	>12
					GMA4C_V10	582	>12	6	>12
GMA5CA_WT	586	6	12	>12
					GMA5CA_V3	19	3	6	>12
GMA5CA_V4	20	3	6	>12

The in-half plant antifungal activity of the gma4c_v9, gma4c_v10, GMAOe c_wt, gmao1c_v3, GMAOe c_v4, gma1c_v1, and gma1c_v2 peptides against botrytis was determined using isolated benthamiana leaves. Each peptide at concentrations of 1.5 μm, 3 μm and 6 μm was applied to the leaves as droplets and freshly prepared conidium inoculum was immediately applied to each droplet of peptide. By measuring lesion size, the reduction of gray mold symptoms in leaves after 48h inoculation relative to no peptide control was assessed. Both gma4c_v9 and gma4c_v10 peptides were effective in reducing gray mold symptoms. However, at low concentrations of 1.5 μm and 3 μm, gma4c_v10 was more effective in reducing gray mold symptoms than gma4c_v9 (fig. 5).

Instillation inoculation assays were also performed to test the antifungal activity of GMAOe c_wt, gmaoe1c_v1, GMAOe c_v2. The results revealed that GMAOe C_v1 and GMAOe C_v2 at 3 and 6 μM concentrations completely eliminated gray mold symptoms, but GMAOe C_WT was effective only at 6 μM. GMAOe c_v3 was more effective than GMAOe c_v4 or GMAOe c_wt at a concentration of 1.5 μm (fig. 6).

Instillation inoculation assays were also performed to test the antifungal activity of gma1c_v1 and gma1c_v2. (FIG. 6). The 3. Mu.M and 6. Mu.M GMA1C_V2 completely eliminated gray mold symptoms. Gma1c_v1 failed to reduce disease symptoms at these concentrations. At a concentration of 1.5. Mu.M, GMA1C_V2 is more effective than GMA1C_V1 (FIG. 7).

The above results indicate that a set of modifications (e.g., amino acid substitutions) introduced into these truncated defensin-derived peptides confers greater antifungal activity than the wild-type truncated peptide.

The breadth and scope of the present disclosure should not be limited by any of the above-described examples.

Sequence listing

<110> Shah, Dilip

Tetorya, Meenakshi

<120> Modified antimicrobial peptides

<130> P13511WO00

<150> 63/365,928

<151> 2022-06-06

<150> 63/202,559

<151> 2021-06-16

<160> 591

<170> Patent In version 3.5

<210> 1

<211> 23

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (1)..(1)

<223> Xaa is F, G or is absent

<220>

<221> Variant

<222> (3)..(3)

<223> Xaa is A, G, Y, H, S, R or diaminobutyric acid

<220>

<221> Variant

<222> (5)..(5)

<223> Xaa is H, L, S, N, R, K or diaminobutyric acid

<220>

<221> Variant

<222> (6)..(15)

<223> Xaa is R, K, H, N, D, F, G, Q, P, A, I, L, V, Y, S or

Diaminobutyric acid, wherein Xaa 8, xaa 9, xaa

10. One or more of Xaa 11, xaa 12, xaa 13, xaa 14, and/or Xaa 15 can be absent

<220>

<221> Variant

<222> (16)..(16)

<223> Xaa is C, V, W, Y or F

<220>

<221> Variant

<222> (17)..(17)

<223> Xaa is L, I, W, Y or F

<220>

<221> Variant

<222> (18)..(18)

<223> Xaa is C, V, W, Y or F

<220>

<221> Variant

<222> (19)..(19)

<223> Xaa is K, T, F, W or Y

<220>

<221> Variant

<222> (20)..(20)

<223> Xaa is R, K, T, L, F, Q or diaminobutyric acid

<220>

<221> Variant

<222> (21)..(21)

<223> Xaa is N, H, K, R, P, Q, I, F, W or Y

<220>

<221> Variant

<222> (22)..(22)

<223> Xaa is N, H, K, P, Q, I, F, W, Y or is absent

<220>

<221> Feature not yet classified

<222> (23)..(23)

<223> C optionally amidated

<400> 1

Xaa Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Xaa Xaa Xaa Xaa Xaa Xaa Cys

20

<210> 2

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 2

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His

1 5 10 15

Cys

<210> 3

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 3

Gly Gly Arg Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Ile

1 5 10 15

Cys

<210> 4

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 4

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Ile

1 5 10 15

Cys

<210> 5

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 5

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Val Phe Val Thr Arg Ile

1 5 10 15

Cys

<210> 6

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 6

Phe Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Trp Arg Trp

1 5 10 15

Cys

<210> 7

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (3)..(3)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (5)..(5)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (8)..(10)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (15)..(15)

<223> Xaa is diaminobutyric acid

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 7

Phe Gly Xaa Cys Xaa Gly Phe Xaa Xaa Xaa Cys Phe Cys Trp Xaa Trp

1 5 10 15

Cys

<210> 8

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 8

Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys

1 5 10 15

<210> 9

<211> 13

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(12)

<223> Xaa is any amino acid, wherein Xaa 7 to Xaa 12 are optionally

Existing in

<400> 9

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys

1 5 10

<210> 10

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, wherein Xaa 7 to Xaa 13 are optionally

Existing in

<400> 10

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys

1 5 10

<210> 11

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 11

Phe Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Trp Arg Trp

1 5 10 15

Cys

<210> 12

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (3)..(3)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (5)..(5)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (8)..(10)

<223> Xaa is diaminobutyric acid

<220>

<221> Variant

<222> (15)..(15)

<223> Xaa is diaminobutyric acid

<400> 12

Phe Gly Xaa Cys Xaa Gly Phe Xaa Xaa Xaa Cys Phe Cys Trp Xaa Trp

1 5 10 15

Cys

<210> 13

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 13

Gly Gly Arg Cys Lys Gly Phe Arg Arg Arg Trp Phe Trp Thr Arg Ile

1 5 10 15

Cys

<210> 14

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (1)..(1)

<223> Xaa1 is F, G or is absent

<220>

<221> Variant

<222> (3)..(3)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (5)..(5)

<223> Xaa is K or R

<220>

<221> Variant

<222> (11)..(13)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is T, F, W or Y

<220>

<221> Variant

<222> (15)..(15)

<223> Xaa is R, K or diaminobutyric acid

<220>

<221> Variant

<222> (16)..(16)

<223> Xaa is I, F, W or Y

<220>

<221> Feature not yet classified

<222> (17)..(17)

<223> Cysteine optionally amidated

<400> 14

Xaa Gly Arg Cys Xaa Gly Phe Arg Arg Arg Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Cys

<210> 15

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 15

Gly Gly Arg Cys Lys Gly Phe Arg Arg Arg Trp Tyr Trp Thr Arg Ile

1 5 10 15

Cys

<210> 16

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 16

Gly Ala Cys His Arg Gln Gly Phe Gly Phe Ala Cys Phe Cys Tyr Lys

1 5 10 15

Lys Cys

<210> 17

<211> 19

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (1)..(1)

<223> Xaa is F, G or is absent

<220>

<221> Variant

<222> (3)..(3)

<223> Xaa is A, F, W, Y or is absent

<220>

<221> Variant

<222> (5)..(5)

<223> Xaa is H, R or K

<220>

<221> Variant

<222> (6)..(6)

<223> Xaa is R or K

<220>

<221> Variant

<222> (7)..(7)

<223> Xaa is Q, R or K

<220>

<221> Variant

<222> (10)..(10)

<223> Xaa is G, R or K

<220>

<221> Variant

<222> (11)..(11)

<223> Xaa is F, W, Y, R or K

<220>

<221> Variant

<222> (12)..(12)

<223> Xaa is A, F, W, Y, R or K

<220>

<221> Variant

<222> (13)..(15)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (16)..(16)

<223> Xaa is F, W, Y, R or K

<220>

<221> Variant

<222> (17)..(18)

<223> Xaa is R or K

<220>

<221> Feature not yet classified

<222> (19)..(19)

<400> 17

Xaa Gly Xaa Cys Xaa Xaa Xaa Gly Phe Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Xaa Xaa Cys

<210> 18

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (12)..(14)

<223> Xaa is F, W or Y

<220>

<221> Feature not yet classified

<222> (18)..(18)

<223> Cysteine optionally amidated

<400> 18

Gly Ala Cys His Arg Gln Gly Phe Gly Phe Ala Xaa Xaa Xaa Tyr Lys

1 5 10 15

Lys Cys

<210> 19

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (18)..(18)

<223> Amidation

<400> 19

Gly Ala Cys His Arg Gln Gly Phe Gly Phe Ala Trp Phe Trp Tyr Lys

1 5 10 15

Lys Cys

<210> 20

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (18)..(18)

<223> Amidation

<400> 20

Gly Ala Cys His Arg Gln Gly Phe Gly Phe Ala Phe Phe Phe Tyr Lys

1 5 10 15

Lys Cys

<210> 21

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 21

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro

1 5 10 15

Cys

<210> 22

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (11)..(13)

<223> Xaa is F, W or Y

<220>

<221> Feature not yet classified

<222> (17)..(17)

<223> Cysteine optionally amidated

<400> 22

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Xaa Xaa Xaa Thr Arg Pro

1 5 10 15

Cys

<210> 23

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 23

Gly Gly Arg Cys Arg Gly Phe Arg Arg Arg Trp Phe Trp Thr Arg Pro

1 5 10 15

Cys

<210> 24

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 24

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Cys Tyr Cys

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 25

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (14)..(16)

<223> Xaa is F, W or Y

<220>

<221> Feature not yet classified

<222> (22)..(22)

<223> Tyrosine optionally amidated

<400> 25

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Xaa Xaa Xaa

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 26

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (22)..(22)

<223> Amidation

<400> 26

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Trp Phe Trp

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 27

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 27

Gly Gly Leu Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His

1 5 10 15

Cys

<210> 28

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (11)..(13)

<223> Xaa is F, W or Y

<220>

<221> Feature not yet classified

<222> (17)..(17)

<223> Cysteine optionally amidated

<400> 28

Gly Gly Leu Cys Arg Gly Phe Arg Arg Arg Xaa Xaa Xaa Thr Lys His

1 5 10 15

Cys

<210> 29

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 29

Gly Gly Leu Cys Arg Gly Phe Arg Arg Arg Trp Phe Trp Thr Lys His

1 5 10 15

Cys

<210> 30

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 30

Gly Gly Tyr Cys Ser Ser Arg Arg Gln Ile Cys Lys Cys Thr Leu Gln

1 5 10 15

Cys

<210> 31

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (11)..(13)

<223> Xaa is F, W or Y

<220>

<221> Feature not yet classified

<222> (17)..(17)

<223> Cysteine optionally amidated

<400> 31

Gly Gly Tyr Cys Ser Ser Arg Arg Gln Ile Xaa Xaa Xaa Thr Leu Gln

1 5 10 15

Cys

<210> 32

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 32

Gly Gly Tyr Cys Ser Ser Arg Arg Gln Ile Trp Phe Trp Thr Leu Gln

1 5 10 15

Cys

<210> 33

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(14)

<223> Xaa is F, W or Y

<400> 33

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 34

<211> 15

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(12)

<223> Xaa is any amino acid, xaa7 to Xaa12 are optionally present

<220>

<221> Variant

<222> (13)..(15)

<223> Xaa is F, W or Y

<400> 34

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

<210> 35

<211> 15

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 35

Gly Arg Cys Arg Asp Asp Phe Arg Cys Trp Cys Thr Lys Asn Cys

1 5 10 15

<210> 36

<211> 15

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 36

Gly Arg Cys Arg Ile Leu Phe Arg Cys Phe Cys Thr Lys Asn Cys

1 5 10 15

<210> 37

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 37

Gly His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Lys Pro Cys

1 5 10 15

<210> 38

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 38

Gly Ser Cys Asn Tyr Val Phe Pro Ala His Lys Cys Ile Cys Tyr Phe

1 5 10 15

Pro Cys

<210> 39

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 39

Gly Ala Cys His Val Arg Asn Gly Lys His Met Cys Phe Cys Tyr Phe

1 5 10 15

Asn Cys

<210> 40

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 40

Gly Ala Cys His Tyr Gln Phe Pro Ser Val Lys Cys Phe Cys Thr Gln

1 5 10 15

Asn Cys

<210> 41

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 41

Gly Ala Cys His Arg Gln Gly Ile Gly Phe Ala Cys Phe Cys Lys Lys

1 5 10 15

Lys Cys

<210> 42

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<400> 42

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Phe Phe Trp

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 43

<211> 12

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(12)

<223> Xaa is F, W or Y

<400> 43

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 44

<211> 13

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(12)

<223> Xaa is any amino acid, xaa7 to Xaa12 are optionally present

<220>

<221> Variant

<222> (13)..(13)

<223> Xaa is F, W or Y

<400> 44

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 45

<211> 12

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(12)

<223> Xaa is F, W, Y, L, V, I or M

<400> 45

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 46

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, xaa7 to Xaa13 are optionally present

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is F, W, Y, L, V, I or M

<400> 46

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 47

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(12)

<223> Xaa is L, V, I or M

<220>

<221> Variant

<222> (13)..(13)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is L, V, I or M

<400> 47

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 48

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, xaa7 to Xaa13 are optionally present

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is L, V, I or M

<220>

<221> Variant

<222> (15)..(15)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (16)..(16)

<223> Xaa is L, V, I or M

<400> 48

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

<210> 49

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(12)

<223> Xaa is F, W, Y, L, V, I or M

<220>

<221> Variant

<222> (13)..(13)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is F, W, Y, L, V, I or M

<400> 49

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 50

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, xaa7 to Xaa13 are optionally present

<220>

<221> Variant

<222> (14)..(14)

<223> Xaa is F, W, Y, L, V, I or M

<220>

<221> Variant

<222> (15)..(15)

<223> Xaa is F, W or Y

<220>

<221> Variant

<222> (16)..(16)

<223> Xaa is F, W, Y, L, V, I or M

<400> 50

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

<210> 51

<211> 14

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(11)

<223> Xaa is any amino acid, xaa7 to Xaa11 are optionally present

<220>

<221> Variant

<222> (12)..(14)

<223> Xaa is F, W, Y, L, V, I or M

<400> 51

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10

<210> 52

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, xaa7 to Xaa13 are optionally present

<220>

<221> Variant

<222> (14)..(16)

<223> Xaa is F, W, Y, L, V, I or M

<400> 52

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

<210> 53

<211> 74

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 53

Cys Thr Ser Thr Leu Ile Asn Gly Ser Ser Asp Glu Glu Arg Thr Tyr

1 5 10 15

Ser Phe Ser Pro Thr Thr Ser Pro Phe Asp Pro Arg Ser Leu Asn Gln

20 25 30

Glu Leu Lys Ile Gly Arg Ile Gly Tyr Cys Phe Asp Cys Ala Arg Ala

35 40 45

Cys Met Arg Arg Gly Lys Tyr Ile Arg Thr Cys Ser Phe Glu Arg Lys

50 55 60

Leu Cys Arg Cys Ser Ile Ser Asp Ile Lys

65 70

<210> 54

<211> 57

<212> PRT

<213> Rice (Oryza sativa)

<400> 54

Gly Thr Thr Lys Val Ala Glu Ala Arg His Cys Leu Ser Gln Ser His

1 5 10 15

Arg Phe Lys Gly Met Cys Val Ser Ser Asn Asn Cys Ala Asn Val Cys

20 25 30

Arg Thr Glu Ser Phe Pro Asp Gly Glu Cys Lys Ser His Gly Leu Glu

35 40 45

Arg Lys Cys Phe Cys Lys Lys Val Cys

50 55

<210> 55

<211> 108

<212> PRT

<213> North moxa (ARTEMISIA VULGARIS)

<400> 55

Ala Gly Ser Lys Leu Cys Glu Lys Thr Ser Lys Thr Tyr Ser Gly Lys

1 5 10 15

Cys Asp Asn Lys Lys Cys Asp Lys Lys Cys Ile Glu Trp Glu Lys Ala

20 25 30

Gln His Gly Ala Cys His Lys Arg Glu Ala Gly Lys Glu Ser Cys Phe

35 40 45

Cys Tyr Phe Asp Cys Ser Lys Ser Pro Pro Gly Ala Thr Pro Ala Pro

50 55 60

Pro Gly Ala Ala Pro Pro Pro Ala Ala Gly Gly Ser Pro Ser Pro Pro

65 70 75 80

Ala Asp Gly Gly Ser Pro Pro Pro Pro Ala Asp Gly Gly Ser Pro Pro

85 90 95

Val Asp Gly Gly Ser Pro Pro Pro Pro Ser Thr His

100 105

<210> 56

<211> 50

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 56

Arg Ile Cys Glu Arg Arg Ser Lys Thr Trp Thr Gly Phe Cys Gly Asn

1 5 10 15

Thr Arg Gly Cys Asp Ser Gln Cys Lys Arg Trp Glu Arg Ala Ser His

20 25 30

Gly Ala Cys His Ala Gln Phe Pro Gly Phe Ala Cys Phe Cys Tyr Phe

35 40 45

Asn Cys

50

<210> 57

<211> 96

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 57

Ser Ile Cys Asn Asp Arg Leu Gly Leu Cys Asp Gly Cys Asp Gln Arg

1 5 10 15

Cys Lys Ala Lys His Gly Pro Ser Cys Glu Ser Lys Cys Asp Gly Pro

20 25 30

Val Gly Met Leu Leu Cys Thr Cys Thr Tyr Glu Cys Gly Pro Thr Lys

35 40 45

Leu Cys Asn Gly Gly Leu Gly Asn Cys Gly Glu Ser Cys Asn Glu Gln

50 55 60

Cys Cys Asp Arg Asn Cys Ala Gln Arg Tyr Asn Gly Gly His Gly Tyr

65 70 75 80

Cys Asn Thr Leu Asp Asp Phe Ser Leu Cys Leu Cys Lys Tyr Pro Cys

85 90 95

<210> 58

<211> 46

<212> PRT

<213> Tendril peanut (Arachis duranensis)

<400> 58

Lys Leu Cys Asn His Leu Ala Asp Thr Tyr Arg Gly Pro Cys Phe Thr

1 5 10 15

Asn Ala Ser Cys Asp Asp His Cys Lys Asn Lys Glu His Phe Val Ser

20 25 30

Gly Thr Cys Met Lys Met Ala Cys Trp Cys Ala His Asn Cys

35 40 45

<210> 59

<211> 57

<212> PRT

<213> Fructus Viticis negundo (Malus baccata)

<400> 59

Arg Thr Glu Ser Ser Lys Ala Ile Glu Gly Lys Ile Cys Glu Phe Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Leu Leu Thr

35 40 45

Arg Thr Cys Met Cys Thr Lys Lys Cys

50 55

<210> 60

<211> 55

<212> PRT

<213> Rice (Oryza sativa)

<400> 60

Leu Ala Pro Ala Gln Ser Ser Val Arg Pro Cys Glu Thr Lys Ser Leu

1 5 10 15

Arg Phe Glu Gly Phe Cys Met Val Glu Asp Asn Cys Ala Asn Val Cys

20 25 30

Arg Thr Glu Gly Phe Val Asp Gly Arg Cys Ser Thr Phe Val Arg Asn

35 40 45

Cys Ile Cys Ile Lys Pro Cys

50 55

<210> 61

<211> 45

<212> PRT

<213> Wild two-grain wheat (Triticum dicoccoides)

<400> 61

Arg Asp Cys Thr Ser Gln Ser His Lys Phe Val Gly Leu Cys Leu Ser

1 5 10 15

Asp Arg Asn Cys Ala Ser Val Cys Leu Thr Glu Tyr Phe Thr Gly Gly

20 25 30

Lys Cys Asp His Arg Arg Cys Val Cys Thr Lys Gly Cys

35 40 45

<210> 62

<211> 47

<212> PRT

<213> Autumn pear (Pyrus ussuriensis) hybrid American pear (Pyrus communication)

<400> 62

Lys Ile Cys Glu Val Pro Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Ser Asn Asn Cys Lys His Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly

20 25 30

His Cys Ser Val Leu Thr Arg Ala Cys Val Cys Thr Lys Lys Cys

35 40 45

<210> 63

<211> 46

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 63

Ala Ile Cys Lys Lys Pro Ser Lys Phe Phe Lys Gly Ala Cys Gly Arg

1 5 10 15

Asp Ala Asp Cys Glu Lys Ala Cys Asp Gln Glu Asn Trp Pro Gly Gly

20 25 30

Val Cys Val Pro Phe Leu Arg Cys Glu Cys Gln Arg Ser Cys

35 40 45

<210> 64

<211> 47

<212> PRT

<213> Triarrhena (Miscanthus lutarioriparius)

<400> 64

Arg Val Cys Arg Arg Arg Ile Ala Gly Phe Lys Gly Val Cys Met Ser

1 5 10 15

Asp His Asn Cys Ala Gln Val Cys Leu Gln Glu Gly Trp Gly Gly Gly

20 25 30

Asn Cys Asp Gly Ile Leu Arg Gln Cys Lys Cys Ile Arg Gln Cys

35 40 45

<210> 65

<211> 103

<212> PRT

<213> Baimaigen (Lotus japonica)

<400> 65

Asn Thr Cys Ser Glu Ala Leu Asp Gln Cys Asp Ser Met Glu Ser Cys

1 5 10 15

Asp Gln Lys Cys Lys Gly Arg His Asn Gly Arg Arg Gly Ser Cys Asn

20 25 30

Leu Gly Leu Cys Thr Cys Ser Tyr Ser Cys Gly Pro Pro Gly Pro Lys

35 40 45

Ile Pro Ser Asn Leu Cys Thr Ala Gly Leu Gly Leu Cys Ser Thr Lys

50 55 60

Cys Gly Asp Ser Tyr Cys Asn Ser Ile Cys Ala Ser Lys Tyr Asn Gln

65 70 75 80

Gly Val Gly Phe Cys Tyr Thr Ser Gly Pro Ser Ile Asn Leu Cys Thr

85 90 95

Cys Gln Tyr Pro Cys Ala Leu

100

<210> 66

<211> 49

<212> PRT

<213> Meili drill vegetable (Aquilegia formosa)

<400> 66

Arg Asp Cys Glu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Ile Arg

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Ser Glu Gly Phe Asn Gly Gly

20 25 30

His Cys Arg Gly Val Thr Arg Arg Cys Tyr Cys Thr Ala Lys Cys His

35 40 45

Glu

<210> 67

<211> 49

<212> PRT

<213> Dichanthelium oligosanthes

<400> 67

Arg His Cys Leu Ser Pro Ser His His Phe Lys Gly Leu Cys Met Ser

1 5 10 15

Ser Asn Asn Cys Ala Asn Val Cys Arg Thr Glu Asn Phe Pro Gly Gly

20 25 30

Glu Cys Lys Thr Glu Gly Ala Thr Arg Lys Cys Met Cys Lys Lys Val

35 40 45

Cys

<210> 68

<211> 56

<212> PRT

<213> Arabis nemorensis

<400> 68

Cys Thr Thr Ser Ile Gly Pro Cys Glu Val Gly Lys Asn Cys Ser Pro

1 5 10 15

Lys Cys Asn Ala Met Phe Gly Glu Ile Val Thr Gly Phe Cys Asp Arg

20 25 30

Ser Gly Gly Gly Ile Gly Ile Cys Val Cys Val Thr Pro Cys Pro Pro

35 40 45

Pro Pro Pro Lys Ala Pro His Met

50 55

<210> 69

<211> 99

<212> PRT

<213> Sesame (Sesamum indicum)

<400> 69

Ala Val Cys Gln Thr Arg Ser Gln Gln Phe Arg Gly Leu Cys Phe Arg

1 5 10 15

Asn Asp Asn Cys Ala Ala Ile Cys Glu Lys Glu Gly Tyr Ile Ser Gly

20 25 30

Ser Cys Lys Gly Phe Leu Leu Arg Cys Ile Cys Phe Lys Asp Cys Gly

35 40 45

Ala Thr Gly Gly Gly Pro Pro Asp Gly Ser Pro Asp Gly Asp Gly Gly

50 55 60

Thr Pro Glu Gly Leu Thr Gly Ser His Val Thr Ser Asn Ile Ser Ser

65 70 75 80

Val Tyr Lys Tyr Gly Lys Asn Glu Ala Lys Pro Gly Asn Ala Glu Ile

85 90 95

Lys Lys Gly

<210> 70

<211> 45

<212> PRT

<213> Turnip (Brassica rapa)

<400> 70

Arg Thr Cys Glu Ser Pro Ser Thr Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ala Lys Asn Cys Ala Gly Val Cys Thr Gly Glu Gly Phe Thr Gly Gly

20 25 30

Gln Cys Ser Ser Leu Lys Cys Tyr Cys Thr Lys Thr Cys

35 40 45

<210> 71

<211> 49

<212> PRT

<213> Shui Qing Tree (Tetracentron sinense)

<400> 71

Lys Thr Cys Thr Lys Arg Ser Arg Thr Trp Phe Gly Leu Cys Leu Asn

1 5 10 15

Ser Asn Gly Cys Asn Arg Gln Cys Lys Lys Trp Glu Gly Ala Lys His

20 25 30

Gly Ala Cys His Ala Gln Phe Gln Gly Thr Ala Cys Phe Cys Tyr Tyr

35 40 45

Cys

<210> 72

<211> 71

<212> PRT

<213> Microthlaspi erraticum

<400> 72

Arg Glu Thr Cys Asp Lys Pro Tyr Ser Ile Cys Trp Phe Trp Pro Phe

1 5 10 15

Phe Ser Asp Ser Asp Ala Tyr Cys Arg Glu Ser Cys Leu Lys Val Lys

20 25 30

Asp Glu Lys Tyr Glu Tyr Tyr Asp Gly Gln Cys Arg Pro Ala Arg Ser

35 40 45

Ala Leu Pro Leu Pro Pro Glu Cys Phe Cys Cys Val Arg Glu Arg Ser

50 55 60

Arg Ala Glu Ile Glu Ser Ile

65 70

<210> 73

<211> 62

<212> PRT

<213> Vanilla (Vanilla planifolia)

<400> 73

Arg Arg Cys Arg Ala Gln Ser Phe Thr Phe Lys Gly Ile Cys Tyr Ser

1 5 10 15

Ser His Ser Cys Ala Leu Val Cys Lys Lys Glu Gly Tyr Glu Thr Gly

20 25 30

His Cys Ser Arg Arg Glu Gln His Lys Ile Phe Cys Ile Cys Ala Phe

35 40 45

Lys Ser Leu Asn Gln Thr Lys Asp Thr Leu Gln Glu Leu Asp

50 55 60

<210> 74

<211> 47

<212> PRT

<213> Soybean (Glycine max)

<400> 74

Lys Asp Cys Leu Thr Arg Arg His Gly Phe Gln Gly Arg Cys Leu Phe

1 5 10 15

Asp Arg Gln Cys Ala His Val Cys Arg Ser Asp Gly Phe Ile Gly Gly

20 25 30

Gln Cys Arg Gly Pro Leu Arg Lys Cys Phe Cys Ser Arg Pro Cys

35 40 45

<210> 75

<211> 49

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 75

Arg His Cys Leu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Val Arg

1 5 10 15

Ser Gly Asn Cys Ala Asn Val Cys Lys Thr Glu Asn Phe Pro Asp Gly

20 25 30

Glu Cys Lys Thr Gln Gly Leu Glu Arg Lys Cys Phe Cys Lys Arg Val

35 40 45

Cys

<210> 76

<211> 49

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 76

Lys Leu Cys Glu Glu Arg Ser Gln Lys Phe Arg Gly Pro Cys Lys Phe

1 5 10 15

Asn Thr Asn Cys Val Ala Val Cys Val Thr Glu Gly Tyr Thr Gly Gly

20 25 30

Tyr Cys His Lys Thr Val Phe His Ser His Cys Met Cys Thr Lys Glu

35 40 45

Cys

<210> 77

<211> 47

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 77

Lys Ile Cys Arg Lys Arg Ser Ala Gly Tyr His Gly Met Cys Met Ser

1 5 10 15

Asp Arg Asn Cys Ala Gln Val Cys Gln Ala Glu Gly Trp Gly Gly Gly

20 25 30

Asn Cys Asp Gly Ala Val Arg Arg Cys Lys Cys Ser Arg Glu Cys

35 40 45

<210> 78

<211> 47

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 78

Arg Glu Cys Glu Thr Glu Ser Thr Lys Phe Glu Gly Leu Cys Ile Met

1 5 10 15

His Ser His Cys Pro Asp Val Cys Val Thr Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Ser Thr Trp Lys Arg Lys Cys Met Cys Thr Lys Glu Cys

35 40 45

<210> 79

<211> 48

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 79

Lys Lys Cys Ser Lys Pro Ser Gly Arg Phe Lys Gly Pro Cys Phe Phe

1 5 10 15

Lys Val Asp Cys Glu Gln Gln Cys Lys Lys Glu Gly Trp Pro Arg Gly

20 25 30

Val Cys Thr Gly Phe Ala Gly Arg Cys Val Cys Lys Asn Tyr Gly Cys

35 40 45

<210> 80

<211> 146

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 80

Tyr Val His Gly Phe Cys Asn Ser Pro Ser Gly Ser Phe Arg Gly Pro

1 5 10 15

Cys Phe Ser Asp Ser His Cys Lys Ala Ala Cys Glu His Glu Tyr Tyr

20 25 30

Lys Gly Asp Lys Ser Lys Pro Phe Thr Asp Gly Lys Cys Ser Asp Ser

35 40 45

Gly Ser Gln Arg Leu Asp Gly Val Gly Val Gly Ser Arg Trp Ser Leu

50 55 60

Leu Pro Thr Cys Ile Cys Ser Ile Phe Cys Gly Ser Glu Lys Ile Thr

65 70 75 80

Trp Arg Pro Ile Ile Thr Ile Arg Leu Arg Cys Gln Lys Thr Gln Asn

85 90 95

Arg Arg Leu Arg Thr Thr Ile Thr Ile Arg Leu Arg Arg Gln Lys Ala

100 105 110

Gln Asn Arg Arg Arg Arg Thr Ile Thr Ile Arg Leu Arg Arg Arg Asn

115 120 125

Lys Ser Arg Gln His Lys Ala Ala Ser Arg Arg Gly Lys Cys Trp Glu

130 135 140

Thr Ser

145

<210> 81

<211> 103

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 81

Leu His Trp Phe Cys Asn Ile Pro Ser Gly Ser Phe His Gly Pro Cys

1 5 10 15

Met Ser Asn Asp His Cys Leu Ala Ala Cys Lys Ser Glu Tyr Tyr Asn

20 25 30

Gly Asp Lys Ser Lys Pro Phe Thr Gly Gly Gln Cys Ser Leu Ser Gly

35 40 45

Ser Ile Leu Pro Arg Cys Ile Cys Ser Ile Phe Cys Gly Pro Pro Met

50 55 60

Ala Glu His Asp Asp Pro Glu Thr His Arg His His His Pro Pro Leu

65 70 75 80

Pro Pro Pro Ser Glu Gly Pro Glu Leu Ser Pro Pro His His His Ala

85 90 95

Pro Pro Pro Arg His His His

100

<210> 82

<211> 47

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 82

Arg Asp Cys Glu Ala Pro Ser His Lys Phe His Gly Ala Cys Val Arg

1 5 10 15

Asp Ser Asn Cys Ala Ser Val Cys Glu Thr Glu Gly Phe Ser Gly Asp

20 25 30

Lys Arg Glu Gly Pro Leu Met Arg Cys Tyr Cys Thr Lys Gln Cys

35 40 45

<210> 83

<211> 81

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 83

Gly Asp Glu Leu Leu Cys Asp Val Arg Leu Thr Lys Cys Glu Asp Val

1 5 10 15

Cys Tyr Lys Ser Gly Lys Cys Ser Arg Cys Cys Lys Asn His Arg Phe

20 25 30

Asn His Gly His Cys Asp Leu Lys Ser His Cys Tyr Cys Tyr Arg Gly

35 40 45

Pro Asn Ser Gly Ala Gly Asp Gly Glu Gln Gln Arg Met Leu Arg Val

50 55 60

Ile Pro Ala Ser Pro Pro Pro Pro Ala Ala Arg His Pro Pro Leu His

65 70 75 80

Ala

<210> 84

<211> 49

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 84

Thr Leu Cys Asn Leu Arg Ser Met Gly Phe Val Gly Val Cys Trp Ser

1 5 10 15

Asn Met Ser Cys Ala Asn Gln Cys Val Leu Glu Gly Arg Thr Ser Gly

20 25 30

Tyr Cys Lys Gly Ile Pro Ala Ile Lys Tyr Cys Met Cys Thr Phe Glu

35 40 45

Cys

<210> 85

<211> 47

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 85

Asp Arg Cys Glu Thr His Ser Arg Thr Tyr Lys Gly Arg Cys Asn Asn

1 5 10 15

His Asn Cys Trp Ser Ile Cys Ile Thr Glu Gly Asn Thr Gly Gly Phe

20 25 30

Cys Lys Gly Thr Leu Leu Leu Lys Cys Met Cys Thr Ser Glu Cys

35 40 45

<210> 86

<211> 48

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 86

Asp Met Cys Lys Lys Arg Ser Arg Thr Phe Glu Gly Arg Cys Gly Leu

1 5 10 15

Asn Met Asn Cys Ala Thr Val Cys Val Ala Glu His Tyr Thr Gly Gly

20 25 30

Phe Cys Lys Gly Phe Phe His Arg Glu Cys Met Cys Thr Lys Asp Cys

35 40 45

<210> 87

<211> 50

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 87

Arg Arg Cys Val Ser Gln Ser His Lys Phe Val Gly Ser Cys Met Arg

1 5 10 15

Lys Ser Asn Cys Gln His Val Cys Gln Thr Glu Gly Phe Pro Trp Gly

20 25 30

Glu Cys Arg Phe His Gly Gly Leu Leu Arg Arg Cys Phe Cys Asn Lys

35 40 45

Leu Cys

50

<210> 88

<211> 29

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 88

Gly Tyr Cys Phe Asp Cys Ala Arg Ala Cys Met Arg Arg Gly Lys Tyr

1 5 10 15

Ile Arg Thr Cys Ser Phe Glu Arg Lys Leu Cys Arg Cys

20 25

<210> 89

<211> 18

<212> PRT

<213> Japonica rice (Oryza sativa Japonica)

<400> 89

Gly Glu Cys Lys Ser His Gly Leu Glu Arg Lys Cys Phe Cys Lys Lys

1 5 10 15

Val Cys

<210> 90

<211> 19

<212> PRT

<213> North moxa (ARTEMISIA VULGARIS)

<400> 90

Gly Ala Cys His Lys Arg Glu Ala Gly Lys Glu Ser Cys Phe Cys Tyr

1 5 10 15

Phe Asp Cys

<210> 91

<211> 18

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 91

Gly Ala Cys His Ala Gln Phe Pro Gly Phe Ala Cys Phe Cys Tyr Phe

1 5 10 15

Asn Cys

<210> 92

<211> 18

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 92

Gly Tyr Cys Asn Thr Leu Asp Asp Phe Ser Leu Cys Leu Cys Lys Tyr

1 5 10 15

Pro Cys

<210> 93

<211> 14

<212> PRT

<213> Tendril peanut (Arachis duranensis)

<400> 93

Gly Thr Cys Met Lys Met Ala Cys Trp Cys Ala His Asn Cys

1 5 10

<210> 94

<211> 16

<212> PRT

<213> Fructus Viticis negundo (Malus baccata)

<400> 94

Gly Lys Cys Ser Leu Leu Thr Arg Thr Cys Met Cys Thr Lys Lys Cys

1 5 10 15

<210> 95

<211> 16

<212> PRT

<213> Rice (Oryza sativa)

<400> 95

Gly Arg Cys Ser Thr Phe Val Arg Asn Cys Ile Cys Ile Lys Pro Cys

1 5 10 15

<210> 96

<211> 14

<212> PRT

<213> Wild two-grain wheat (Triticum dicoccoides)

<400> 96

Gly Lys Cys Asp His Arg Arg Cys Val Cys Thr Lys Gly Cys

1 5 10

<210> 97

<211> 16

<212> PRT

<213> Autumn pear (Pyrus ussuriensis) hybrid American pear (Pyrus communication)

<400> 97

Gly His Cys Ser Val Leu Thr Arg Ala Cys Val Cys Thr Lys Lys Cys

1 5 10 15

<210> 98

<211> 15

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 98

Gly Val Cys Val Pro Phe Leu Arg Cys Glu Cys Gln Arg Ser Cys

1 5 10 15

<210> 99

<211> 16

<212> PRT

<213> Triarrhena (Miscanthus lutarioriparius)

<400> 99

Gly Asn Cys Asp Gly Ile Leu Arg Gln Cys Lys Cys Ile Arg Gln Cys

1 5 10 15

<210> 100

<211> 21

<212> PRT

<213> Baimaigen (Lotus japonica)

<400> 100

Gly Phe Cys Tyr Thr Ser Gly Pro Ser Ile Asn Leu Cys Thr Cys Gln

1 5 10 15

Tyr Pro Cys Ala Leu

20

<210> 101

<211> 18

<212> PRT

<213> Meili drill vegetable (Aquilegia formosa)

<400> 101

Gly His Cys Arg Gly Val Thr Arg Arg Cys Tyr Cys Thr Ala Lys Cys

1 5 10 15

His Glu

<210> 102

<211> 18

<212> PRT

<213> Dichanthelium oligosanthes

<400> 102

Gly Glu Cys Lys Thr Glu Gly Ala Thr Arg Lys Cys Met Cys Lys Lys

1 5 10 15

Val Cys

<210> 103

<211> 29

<212> PRT

<213> Arabis nemorensis

<400> 103

Gly Phe Cys Asp Arg Ser Gly Gly Gly Ile Gly Ile Cys Val Cys Val

1 5 10 15

Thr Pro Cys Pro Pro Pro Pro Pro Lys Ala Pro His Met

20 25

<210> 104

<211> 16

<212> PRT

<213> Sesame (Sesamum indicum)

<400> 104

Gly Ser Cys Lys Gly Phe Leu Leu Arg Cys Ile Cys Phe Lys Asp Cys

1 5 10 15

<210> 105

<211> 14

<212> PRT

<213> Turnip (Brassica rapa)

<400> 105

Gly Gln Cys Ser Ser Leu Lys Cys Tyr Cys Thr Lys Thr Cys

1 5 10

<210> 106

<211> 17

<212> PRT

<213> Shui Qing Tree (Tetracentron sinense)

<400> 106

Gly Ala Cys His Ala Gln Phe Gln Gly Thr Ala Cys Phe Cys Tyr Tyr

1 5 10 15

Cys

<210> 107

<211> 18

<212> PRT

<213> Microthlaspi erraticum

<400> 107

Gly Gln Cys Arg Pro Ala Arg Ser Ala Leu Pro Leu Pro Pro Glu Cys

1 5 10 15

Phe Cys

<210> 108

<211> 15

<212> PRT

<213> Vanilla (Vanilla planifolia)

<400> 108

Gly His Cys Ser Arg Arg Glu Gln His Lys Ile Phe Cys Ile Cys

1 5 10 15

<210> 109

<211> 17

<212> PRT

<213> Soybean (Glycine max)

<400> 109

Gly Gly Gln Cys Arg Gly Pro Leu Arg Lys Cys Phe Cys Ser Arg Pro

1 5 10 15

Cys

<210> 110

<211> 18

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 110

Gly Glu Cys Lys Thr Gln Gly Leu Glu Arg Lys Cys Phe Cys Lys Arg

1 5 10 15

Val Cys

<210> 111

<211> 18

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 111

Gly Tyr Cys His Lys Thr Val Phe His Ser His Cys Met Cys Thr Lys

1 5 10 15

Glu Cys

<210> 112

<211> 16

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 112

Gly Asn Cys Asp Gly Ala Val Arg Arg Cys Lys Cys Ser Arg Glu Cys

1 5 10 15

<210> 113

<211> 16

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 113

Gly Lys Cys Ser Thr Trp Lys Arg Lys Cys Met Cys Thr Lys Glu Cys

1 5 10 15

<210> 114

<211> 17

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 114

Gly Val Cys Thr Gly Phe Ala Gly Arg Cys Val Cys Lys Asn Tyr Gly

1 5 10 15

Cys

<210> 115

<211> 32

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 115

Gly Lys Cys Ser Asp Ser Gly Ser Gln Arg Leu Asp Gly Val Gly Val

1 5 10 15

Gly Ser Arg Trp Ser Leu Leu Pro Thr Cys Ile Cys Ser Ile Phe Cys

20 25 30

<210> 116

<211> 19

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 116

Gly Gln Cys Ser Leu Ser Gly Ser Ile Leu Pro Arg Cys Ile Cys Ser

1 5 10 15

Ile Phe Cys

<210> 117

<211> 26

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 117

Ser Val Cys Glu Thr Glu Gly Phe Ser Gly Asp Lys Arg Glu Gly Pro

1 5 10 15

Leu Met Arg Cys Tyr Cys Thr Lys Gln Cys

20 25

<210> 118

<211> 25

<212> PRT

<213> Break grass (Brachypodium distachyon)

<400> 118

Gly Lys Cys Ser Arg Cys Cys Lys Asn His Arg Phe Asn His Gly His

1 5 10 15

Cys Asp Leu Lys Ser His Cys Tyr Cys

20 25

<210> 119

<211> 18

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 119

Gly Tyr Cys Lys Gly Ile Pro Ala Ile Lys Tyr Cys Met Cys Thr Phe

1 5 10 15

Glu Cys

<210> 120

<211> 17

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 120

Gly Phe Cys Lys Gly Thr Leu Leu Leu Lys Cys Met Cys Thr Ser Glu

1 5 10 15

Cys

<210> 121

<211> 17

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 121

Gly Phe Cys Lys Gly Phe Phe His Arg Glu Cys Met Cys Thr Lys Asp

1 5 10 15

Cys

<210> 122

<211> 19

<212> PRT

<213> Millet (SETARIA ITALICA)

<400> 122

Gly Glu Cys Arg Phe His Gly Gly Leu Leu Arg Arg Cys Phe Cys Asn

1 5 10 15

Lys Leu Cys

<210> 123

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 123

Asp Ile Cys Thr Thr Pro Pro Arg Arg Tyr Ile Gly Val Cys Trp Ser

1 5 10 15

Asp Thr Ser Cys Arg Lys Val Cys Val Glu Met Glu Asn Phe Glu Asp

20 25 30

Gly Tyr Cys Ser Lys Ile Gln Arg Lys Cys Leu Cys Thr Trp Thr Cys

35 40 45

Val Leu Asp Asn Ile Pro Asn Asp Ala Gly Thr Ile Met Leu Gln Asp

50 55 60

Ala Lys Thr Met Gln Leu Leu Glu Glu Lys Phe

65 70 75

<210> 124

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 124

Tyr Asn Ile Cys Lys Thr Lys Ser Lys Tyr Phe Glu Gly Leu Cys Trp

1 5 10 15

Val Asp Ser Ser Cys Arg Lys Val Cys Ile Glu Lys Asp Lys Phe Glu

20 25 30

Asp Gly His Cys Ser Lys Leu Leu Arg Asn Cys Leu Cys Thr Lys Ile

35 40 45

Cys Ala Phe Asp Asn Ile Pro Asn Asp Ala Gly Thr Ile Leu Val Gln

50 55 60

Asp Ala Lys Ser Leu Glu Ala Gln Leu Leu Glu

65 70 75

<210> 125

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 125

Gln Asn Asn Ile Cys Lys Thr Thr Ser Lys His Phe Lys Gly Leu Cys

1 5 10 15

Phe Ala Asp Ser Lys Cys Arg Lys Val Cys Ile Gln Glu Asp Lys Phe

20 25 30

Glu Asp Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys

35 40 45

Asn Cys Val Phe Asp Asn Ile Pro Asn Asp Val Gly Thr Ile Leu Val

50 55 60

Glu Asp Ala Lys Thr Leu Glu Ala Gln Leu Leu

65 70 75

<210> 126

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 126

Gln Gln Thr Cys Lys Thr Glu Ser Gln Lys Phe Lys Gly Leu Cys Ile

1 5 10 15

Thr Lys Ser Ser Cys Arg Lys Ala Cys Leu Lys Glu Lys Phe Thr Asp

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

Val Phe Glu Asn Asn Pro Asn Glu Val Glu Thr Lys Leu Val Gly Lys

50 55 60

Thr Lys Thr Leu Arg Glu Ala Met Leu Glu Glu

65 70 75

<210> 127

<211> 79

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 127

Arg Gln Thr Cys Lys Thr Glu Ser Gln Lys Phe Lys Gly Leu Cys Ile

1 5 10 15

Thr Lys Ser Ser Cys Arg Lys Ala Cys Leu Lys Glu Lys Phe Thr Asp

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

Val Phe Glu Asn Asn Pro Asn Glu Asp Glu Thr Asn Leu Val Gly Lys

50 55 60

Thr Lys Thr Leu Arg Glu Ala Met Leu Glu Glu Gln Ile Met Glu

65 70 75

<210> 128

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 128

Tyr Asn Ile Cys Lys Thr Lys Ser Lys Lys Phe Glu Gly Leu Cys Phe

1 5 10 15

Leu Asp Ser Ser Cys Arg Lys Val Cys Val Glu Gln Glu Asn Phe Glu

20 25 30

Asp Gly His Cys Ser Lys Leu Arg Arg Leu Cys Leu Cys Thr Lys Leu

35 40 45

Cys Val Phe Asp Asn Ile Pro Asn Asp Val Gly Thr Ile Leu Val Gln

50 55 60

Asp Ala Lys Thr Leu Glu Ala Gln Leu Leu Glu

65 70 75

<210> 129

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 129

Gln Asn Ile Cys Lys Thr Lys Ser Lys Tyr Phe Thr Gly Leu Cys Trp

1 5 10 15

Thr Asp Ser Ser Cys Arg Lys Val Cys Ile Glu Lys Asp Lys Phe Gln

20 25 30

Asp Gly His Cys Ser Lys Leu Gln Arg Asn Cys Leu Cys Thr Lys Ile

35 40 45

Cys Val Phe Asp Asn Ile Pro Asn Asp Ala Gly Thr Ile Leu Val Gln

50 55 60

Asp Ala Lys Thr Asn Glu Ala Gln Leu Leu Glu

65 70 75

<210> 130

<211> 75

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 130

Tyr Asn Ile Cys Lys Thr Lys Ser Lys Tyr Phe Glu Gly Leu Cys Trp

1 5 10 15

Val Asp Ser Ser Cys Arg Lys Val Cys Ile Glu Lys Asp Lys Phe Glu

20 25 30

Asp Gly His Cys Ser Lys Leu Leu Arg Asn Cys Leu Cys Thr Lys Ile

35 40 45

Cys Ala Phe Asp Asn Ile Pro Asn Asp Ala Gly Thr Ile Leu Val Gln

50 55 60

Asp Ala Lys Ser Leu Glu Ala Gln Leu Leu Glu

65 70 75

<210> 131

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 131

Arg Glu Cys Lys Thr Glu Ser Asn Thr Phe Pro Gly Leu Cys Ile Thr

1 5 10 15

Lys Pro Pro Cys Arg Lys Ala Cys Ile Ser Glu Gly Phe Thr Asp Gly

20 25 30

His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Lys Pro Cys Val

35 40 45

Phe Asp Glu Lys Met Ile Lys Thr Gly Ala Glu Thr Leu Ala Glu Glu

50 55 60

Ala Lys Thr Leu Ala Ala Ala Leu Phe Glu Glu

65 70 75

<210> 132

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 132

Arg Glu Cys Lys Thr Glu Ser Asn Thr Phe Pro Gly Leu Cys Ile Thr

1 5 10 15

Lys Pro Pro Cys Arg Lys Ala Cys Ile Ser Glu Gly Phe Thr Asp Gly

20 25 30

His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Lys Pro Cys Val

35 40 45

Phe Asp Glu Lys Met Ile Lys Thr Gly Ala Glu Thr Phe Ala Glu Glu

50 55 60

Ala Lys Thr Leu Ala Ala Ala Leu Leu Glu Glu

65 70 75

<210> 133

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 133

Arg Glu Cys Lys Ala Val Ser Asn Thr Leu Ser Gly Leu Cys Ile Thr

1 5 10 15

Lys Pro Pro Cys Arg Glu Ala Cys Ile Ser Glu Gly Phe Thr Asp Gly

20 25 30

Lys Cys Ser Lys Ile Leu Lys Arg Cys Ile Cys His Lys Pro Cys Val

35 40 45

Phe Asp Glu Lys Met Val Lys Thr Gly Ala Glu Thr Leu Ala Glu Glu

50 55 60

Val Lys Thr Leu Ala Ala Ala Phe Leu Glu Glu

65 70 75

<210> 134

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 134

Arg Glu Cys Lys Ala Glu Ser Ile Thr Phe Ser Gly Leu Cys Val Ser

1 5 10 15

Lys Pro Pro Cys Arg Lys Ala Cys Ile Cys Glu Gly Phe Thr Asn Gly

20 25 30

His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Thr Pro Ser Val

35 40 45

Phe Asp Glu Lys Met Ile Lys Thr Gly Ala Glu Thr Leu Gly Glu Glu

50 55 60

Ala Lys Thr Leu Ala Ala Thr Leu Leu Glu Glu

65 70 75

<210> 135

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 135

Gln Ser Thr Cys Lys Ala Glu Ser Asn Thr Phe Pro Gly Ile Cys Ile

1 5 10 15

Thr Lys Pro Pro Cys Arg Lys Ala Cys Leu Ser Glu Gly Phe Thr Asp

20 25 30

Gly Lys Cys Ser Lys Ile Leu Arg Arg Cys Ile Cys Tyr Lys Pro Cys

35 40 45

Val Phe Asp Glu Lys Met Thr Lys Thr Gly Ala Glu Thr Leu Ala Glu

50 55 60

Glu Ala Lys Thr Leu Ala Ala Ala Leu Leu Glu

65 70 75

<210> 136

<211> 75

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 136

Arg Glu Cys Lys Thr Glu Ser Asn Thr Phe Pro Gly Ile Cys Ile Thr

1 5 10 15

Lys Pro Pro Cys Arg Lys Ala Cys Ile Ser Glu Lys Phe Thr Asp Gly

20 25 30

His Cys Ser Lys Leu Leu Arg Arg Cys Leu Cys Thr Lys Pro Cys Val

35 40 45

Phe Asp Glu Lys Met Ile Lys Thr Gly Ala Glu Thr Leu Val Glu Glu

50 55 60

Ala Lys Thr Leu Ala Ala Ala Leu Leu Glu Glu

65 70 75

<210> 137

<211> 75

<212> PRT

<213> Petunia (Petunia hybrid)

<400> 137

Ala Thr Cys Lys Ala Glu Cys Ala Thr Trp Glu Gly Ile Cys Ile Asn

1 5 10 15

Lys Ala Pro Cys Val Lys Cys Cys Lys Glu Gln Pro Glu Asn Phe Thr

20 25 30

Asp Gly His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Lys Pro

35 40 45

Cys Ala Thr Ala Glu Ala Thr Ala Thr Leu Ala Asn Glu Val Lys Thr

50 55 60

Met Gly Glu Ala Leu Val Glu Val Asp Met Met

65 70 75

<210> 138

<211> 75

<212> PRT

<213> Petunia (Petunia hybrid)

<400> 138

Ala Thr Cys Lys Ala Glu Cys Pro Thr Trp Asp Gly Ile Cys Ile Asn

1 5 10 15

Lys Gly Pro Cys Val Lys Cys Cys Lys Ala Gln Pro Glu Lys Phe Thr

20 25 30

Asp Gly His Cys Ser Lys Val Leu Arg Arg Cys Leu Cys Thr Lys Pro

35 40 45

Cys Ala Thr Glu Glu Ala Thr Ala Thr Leu Ala Asn Glu Val Lys Thr

50 55 60

Met Ala Glu Ala Leu Val Glu Glu Asp Met Met

65 70 75

<210> 139

<211> 75

<212> PRT

<213> Petunia (Petunia hybrid)

<400> 139

Ala Thr Cys Lys Ala Glu Cys Pro Thr Trp Asp Ser Val Cys Ile Asn

1 5 10 15

Lys Lys Pro Cys Val Ala Cys Cys Lys Lys Ala Lys Phe Ser Asp Gly

20 25 30

His Cys Ser Lys Ile Leu Arg Arg Cys Leu Cys Thr Lys Glu Cys Val

35 40 45

Phe Glu Lys Thr Glu Ala Thr Gln Thr Glu Thr Phe Thr Lys Asp Val

50 55 60

Asn Thr Leu Ala Glu Ala Leu Leu Glu Ala Asp

65 70 75

<210> 140

<211> 75

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 140

Gln Gln Ile Cys Lys Ala Pro Ser Gln Thr Phe Pro Gly Leu Cys Phe

1 5 10 15

Met Asp Ser Ser Cys Arg Lys Tyr Cys Ile Lys Glu Lys Phe Thr Gly

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys Pro Cys

35 40 45

Val Phe Asp Lys Ile Ser Ser Glu Val Lys Ala Thr Leu Gly Glu Glu

50 55 60

Ala Lys Thr Leu Ser Glu Val Val Leu Glu Glu

65 70 75

<210> 141

<211> 68

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 141

Gln Pro Met Cys Lys Ser Thr Ser Gln Thr Phe Lys Gly Leu Cys Phe

1 5 10 15

Thr Asp Ser Ser Cys Arg Lys Ser Cys Leu Lys Glu Glu Phe Glu Gly

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys Ile Cys

35 40 45

Val Phe Asp Lys Ile Ser Asn Glu Val Arg Thr Asn Phe Gly Trp Gly

50 55 60

Ser Lys Asn Ser

65

<210> 142

<211> 78

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 142

Ala Ser Ser Thr Arg Ala Asn Gln Gln Ala Lys Ser Ser Lys Asp Tyr

1 5 10 15

Val Leu Pro Thr His His Val Glu Lys Leu Val Ser Lys Arg Ser Leu

20 25 30

Lys Val Asp Ile Ile Phe Lys Leu Gln Ser Lys Cys Leu Cys Thr Lys

35 40 45

Ile Cys Val Phe Glu Lys Asn Pro Asn Glu Val Lys Pro Thr Leu Gly

50 55 60

Gly Glu Ala Lys Thr Leu Ser Glu Thr Met Leu Glu Asp Asp

65 70 75

<210> 143

<211> 62

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 143

Gln His Met Cys Lys Ser Thr Ser Gln Thr Phe Lys Gly Leu Cys Phe

1 5 10 15

Thr Tyr Ser Ser Cys Arg Lys Gly Cys Leu Lys Glu Lys Phe Glu Gly

20 25 30

Gly His Tyr Ile Val Ala Asn Ser Lys Gly Ser Ala Tyr Ala Leu Arg

35 40 45

Phe Val Tyr Leu Thr Lys Phe Gln Met Lys Leu Asn Gln Leu

50 55 60

<210> 144

<211> 75

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 144

Gln Tyr Met Cys Lys Ser Thr Ser Gln Thr Phe Lys Gly Leu Cys Phe

1 5 10 15

Thr Asp Ser Ser Cys Arg Lys Ala Cys Val Thr Glu Glu Phe Thr Gly

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys Val Cys

35 40 45

Val Leu Glu Lys Asp Ser Asn Glu Val Lys Thr Thr Leu Val Gly Glu

50 55 60

Ala Lys Thr Leu Ser Glu Thr Val Leu Glu Glu

65 70 75

<210> 145

<211> 75

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 145

Gln Gln Met Cys Lys Ser Thr Ser Gln Thr Phe Lys Gly Leu Cys Phe

1 5 10 15

Thr Asp Ser Ser Cys Arg Lys Ala Cys Val Thr Glu Glu Phe Thr Gly

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys Val Cys

35 40 45

Val Phe Glu Lys Asp Ser Asn Glu Val Lys Thr Thr Leu Val Gly Glu

50 55 60

Ala Lys Thr Leu Ser Glu Thr Val Leu Glu Glu

65 70 75

<210> 146

<211> 75

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 146

Arg Gln Ile Cys Lys Ala Pro Ser Gln Thr Phe Pro Gly Leu Cys Phe

1 5 10 15

Met Asp Ser Ser Cys Arg Lys Tyr Cys Ile Lys Glu Lys Phe Thr Gly

20 25 30

Gly His Cys Ser Thr Leu Gln Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

Val Phe Asp Ile Ile Ser Asn Glu Val Lys Ala Thr Leu Gly Glu Glu

50 55 60

Ala Lys Thr Leu Ser Glu Val Val Leu Glu Glu

65 70 75

<210> 147

<211> 75

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 147

Gln Gln Met Cys Lys Ser Thr Ser Gln Thr Phe Lys Gly Leu Cys Phe

1 5 10 15

Ile Asp Ser Ser Cys Arg Lys Ala Cys Val Thr Glu Glu Phe Thr Gly

20 25 30

Gly His Cys Ser Lys Leu Gln Arg Lys Cys Leu Cys Thr Lys Val Cys

35 40 45

Val Phe Glu Lys Asp Ser Asn Glu Val Lys Thr Thr Leu Val Gly Glu

50 55 60

Ala Lys Thr Leu Ser Glu Thr Val Leu Glu Glu

65 70 75

<210> 148

<211> 49

<212> PRT

<213> Cajanus cajus

<400> 148

Ala Val Cys Ser Lys Pro Ser Thr Tyr Phe Phe Gly Pro Cys Val Arg

1 5 10 15

Arg Ser Thr Cys Arg Arg Ala Cys Ser His Glu Asn Tyr Pro Asp Gly

20 25 30

Lys Cys Ser Arg Phe Leu Gly Lys Cys Ile Cys Tyr Lys Pro Cys Thr

35 40 45

Thr

<210> 149

<211> 46

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 149

Ala Thr Cys Arg Lys Pro Ser Met Tyr Phe Ser Gly Ala Cys Phe Ser

1 5 10 15

Asp Thr Asn Cys Gln Lys Ala Cys Asn Arg Glu Asp Trp Pro Asn Gly

20 25 30

Lys Cys Leu Val Gly Phe Lys Cys Glu Cys Gln Arg Pro Cys

35 40 45

<210> 150

<211> 55

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 150

Met Asn Ile Val Thr Lys Val Asp Gly Ala Ile Cys Lys Lys Pro Ser

1 5 10 15

Lys Phe Phe Lys Gly Ala Cys Gly Arg Asp Ala Asp Cys Glu Lys Ala

20 25 30

Cys Asp Gln Glu Asn Trp Pro Gly Gly Val Cys Val Pro Phe Leu Arg

35 40 45

Cys Glu Cys Gln Arg Ser Cys

50 55

<210> 151

<211> 49

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 151

Ala Thr Cys Ala Lys Pro Ser Lys Tyr Tyr Lys Gly Gly Cys Phe Ala

1 5 10 15

Thr Ile Ser Val Ser Ala Cys Lys Lys Thr Cys Ser Arg Glu Asn Trp

20 25 30

Pro Asp Gly Ala Cys Val Tyr Pro Phe Arg Cys Glu Cys Arg Arg Pro

35 40 45

Cys

<210> 152

<211> 49

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 152

Lys Thr Cys Ala Lys Pro Ser Lys Tyr Tyr Lys Gly Gly Cys Phe Ser

1 5 10 15

Thr Ile Ser Val Ser Ala Cys Lys Lys Thr Cys Ser Arg Glu Asn Trp

20 25 30

Pro Asp Gly Asp Cys Val Phe Pro Tyr Arg Cys Glu Cys Arg Arg Pro

35 40 45

Cys

<210> 153

<211> 49

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 153

Ala Asn Cys Ala Lys Pro Ser Lys Phe Tyr Arg Gly Pro Cys Phe Asn

1 5 10 15

Ser Ile Ser Val Lys Pro Cys Thr Gln Arg Cys Ser Gln Glu Asn Trp

20 25 30

Pro Ile Gly Val Cys Thr Lys Asp Leu Arg Cys Glu Cys Gln Arg Pro

35 40 45

Cys

<210> 154

<211> 49

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 154

Phe Ser Val Ile Ser Asp Ser Ala Cys Lys Lys Ala Cys Asn Gln Glu

1 5 10 15

Asn Trp Pro Ile Gly Ile Cys Val Pro Val Phe Arg Cys Glu Cys Arg

20 25 30

Arg Ser Cys Gln Ala Met Phe Ser Pro Glu Ser Ser Ser Ile Lys Tyr

35 40 45

Glu

<210> 155

<211> 49

<212> PRT

<213> Cabbage type rape (Brassica napus)

<400> 155

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys Lys Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 156

<211> 47

<212> PRT

<213> Turnip (Brassica rapa)

<400> 156

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 157

<211> 50

<212> PRT

<213> Cabbage type rape (Brassica napus)

<400> 157

Ile Glu Gly Arg Met Cys Gln Ser Arg Ser His His Phe Arg Ser Ile

1 5 10 15

Cys Leu Ser Asn His Asn Cys Ala Asn Ile Cys Arg Gly Glu Arg Phe

20 25 30

Ser Gly Gly Arg Cys His Gly Leu His Arg Lys Cys Tyr Cys Thr Arg

35 40 45

Leu Cys

50

<210> 158

<211> 45

<212> PRT

<213> Turnip (Brassica rapa)

<400> 158

Arg Thr Cys Glu Ser Pro Ser Thr Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ala Lys Asn Cys Ala Gly Val Cys Thr Gly Glu Gly Phe Thr Gly Gly

20 25 30

Gln Cys Ser Ser Leu Lys Cys Tyr Cys Thr Lys Thr Cys

35 40 45

<210> 159

<211> 47

<212> PRT

<213> Critide mustard (Brassica cretica)

<400> 159

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ser Thr Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 160

<211> 47

<212> PRT

<213> Cabbage type rape (Brassica napus)

<400> 160

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Pro Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 161

<211> 48

<212> PRT

<213> Chlorella (Zostera marina)

<400> 161

Arg Thr Cys Val Ser Lys Ser Glu Lys Phe Lys Gly Val Cys Leu Ile

1 5 10 15

Asn Thr Asn Cys Ala His Val Cys Gln Thr Glu Gly Phe Pro Asp Gly

20 25 30

Asp Cys Arg Gly Ser Val Arg Arg Arg Cys Phe Cys Ile Arg Pro Cys

35 40 45

<210> 162

<211> 47

<212> PRT

<213> Microthlaspi erraticum

<400> 162

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Thr His Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 163

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 163

Arg Thr Cys Ala Ser Lys Ser Glu Lys Phe Lys Gly Ser Cys Val Ser

1 5 10 15

Asn Thr Asn Cys Ala Asn Ile Cys His Thr Glu Gly Phe Pro Glu Gly

20 25 30

Ala Cys Arg Gly Leu Arg His His Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 164

<211> 53

<212> PRT

<213> Thlaspi arvensis (THALSPI ARVENSE)

<400> 164

Gln Val Arg Met Cys Glu Ser Lys Ser His Glu Phe Lys Gly Phe Cys

1 5 10 15

Thr Lys Trp Arg Ile Cys Arg Asp Val Cys Ile Thr Glu Gly Phe Thr

20 25 30

Asp Gly Gly Cys His Gly Val Leu Arg His Cys Val Cys Lys Lys Pro

35 40 45

Cys Leu Leu Ser Asn

50

<210> 165

<211> 54

<212> PRT

<213> Kiwi berry (ACTINIDIA CHINENSIS)

<400> 165

Met Ile Lys Gln Gly Glu Ala Arg Val Cys Glu Ser Lys Ser His Gly

1 5 10 15

Phe Lys Gly Ala Cys Leu Ile Asp His Asn Cys Ala Leu Val Cys Arg

20 25 30

Asn Glu Gly Phe Ser Gly Gly Lys Cys Arg Gly Phe Arg His Arg Cys

35 40 45

Phe Cys Thr Lys Ile Cys

50

<210> 166

<211> 63

<212> PRT

<213> Kiwi berry (ACTINIDIA CHINENSIS)

<400> 166

Lys Leu Cys Thr Ala Pro Ser Ser Thr Phe Lys Tyr Leu Cys Ile Ser

1 5 10 15

Asp Gln Asn Cys Ser Ser Ala Cys Glu Gln Glu Gly Phe Thr Ser Gly

20 25 30

Glu Cys Glu Gly Leu Arg Arg Arg Cys Ile Cys Gly Lys Pro Cys Gly

35 40 45

Met Glu Val Ser Lys Thr Lys Phe Asp Asn Gly Ile Gly Gly Glu

50 55 60

<210> 167

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 167

Arg Thr Cys Glu Thr Ser Ser Asn Leu Phe Asn Gly Pro Cys Leu Ser

1 5 10 15

Ser Ser Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Ser Asp Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Leu Cys Thr Arg Pro Cys

35 40 45

<210> 168

<211> 54

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 168

Gln Pro Gln Leu Cys Glu Thr Lys Ser Leu Asn Tyr Arg Gly Leu Cys

1 5 10 15

Met Lys Trp Arg Asn Cys Lys Arg Val Cys Ile Ser Glu Gly Phe Pro

20 25 30

Asp Gly Arg Cys Lys Gly Phe Phe Asn Asn Lys Cys Val Cys Arg Lys

35 40 45

Pro Cys Ala Leu Ser Thr

50

<210> 169

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 169

Arg Thr Cys Ala Ser Gln Ser Gln Arg Phe Lys Gly Lys Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 170

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 170

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Thr His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 171

<211> 45

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 171

Arg Thr Cys Glu Ser Pro Ser Asn Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ser Gln Ser Cys Ala Lys Ala Cys Pro Ser Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Ser Ser Leu Arg Cys Tyr Cys Ser Lys Ala Cys

35 40 45

<210> 172

<211> 59

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 172

Glu Val Ser Pro Val Asp Asn Lys Ile Cys Lys Thr Arg Ser Asp Arg

1 5 10 15

Phe Ser Gly Val Cys Leu Ser Thr Asn Asn Cys Ala Ile Ile Cys Gln

20 25 30

Gln Phe Glu His Phe Asp Gly Gly His Cys Glu Phe Asp Gly Ala Leu

35 40 45

Arg Arg Cys Met Cys Thr Lys Gln Cys Asn Asn

50 55

<210> 173

<211> 54

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 173

Gln Pro Gln Leu Cys Glu Thr Lys Ser Leu Asn Tyr Arg Gly Leu Cys

1 5 10 15

Met Lys Trp Arg Asn Cys Lys Arg Val Cys Ile Ser Glu Gly Phe Pro

20 25 30

Asp Gly Arg Cys Lys Gly Phe Phe Asn Asn Lys Cys Val Cys Arg Lys

35 40 45

Pro Cys Ala Leu Ser Thr

50

<210> 174

<211> 52

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 174

Gln Gln Met Cys Glu Thr Gln Ser Ile Asn Phe Arg Gly Met Cys Thr

1 5 10 15

Lys Trp Arg Asn Cys Gln Arg Val Cys Met Ser Glu Gly Phe Pro Asp

20 25 30

Gly Arg Cys Lys Gly Phe Ile Arg Lys Cys Ile Cys Thr Lys Pro Cys

35 40 45

Pro Val Ser Thr

50

<210> 175

<211> 53

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis lyrata)

<400> 175

Ile Ile Gly Ser Asp Gly Arg Thr Cys Gln Ser Lys Ser His His Phe

1 5 10 15

Lys Tyr Met Cys Thr Ser Ser His Asn Cys Ala Ile Val Cys Arg Asn

20 25 30

Glu Gly Phe Ser Gly Gly Arg Cys His Gly Phe His Arg Arg Cys Tyr

35 40 45

Cys Thr Arg Leu Cys

50

<210> 176

<211> 49

<212> PRT

<213> Fangcalia (Aquilegia oxysepala)

<400> 176

Arg Asp Cys Glu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Ile Arg

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Ser Glu Gly Phe Asn Gly Gly

20 25 30

His Cys Arg Gly Val Thr Arg Arg Cys Tyr Cys Thr Ala Lys Cys His

35 40 45

Glu

<210> 177

<211> 49

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 177

Gly Gly Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Met Cys

1 5 10 15

Val His Glu Arg Asn Cys Ala Ser Val Cys Asn Leu Glu Gly Phe Ser

20 25 30

Gly Gly Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His

35 40 45

Cys

<210> 178

<211> 63

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 178

Glu Gly Arg Lys Cys Asp Ser Gln Ser His His Phe Lys Gly Lys Cys

1 5 10 15

Phe Ser Asp Thr Asn Cys Ala Ser Val Cys His Gly Ser Phe Ile His

20 25 30

Val Phe Val Glu Leu Asp Leu Gln Ser Phe Glu Phe Thr Ser Cys Phe

35 40 45

Ser Leu Glu Ile Lys Lys Phe Ser Ser Arg Ser Gly Gln Leu Phe

50 55 60

<210> 179

<211> 48

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 179

Arg Thr Cys Ala Ser Gln Ser His Arg Phe Lys Gly Val Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Pro Ser Gly

20 25 30

Asp Cys His Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys Ala

35 40 45

<210> 180

<211> 55

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 180

Arg Thr Cys Ala Ser Gln Ser His Arg Phe Lys Gly Val Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Pro Ser Gly

20 25 30

Asp Cys His Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Tyr Arg

35 40 45

Lys Ile Arg Ser Gln Asp Ser

50 55

<210> 181

<211> 49

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 181

Glu Gly Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Met Cys

1 5 10 15

Val His Glu Arg Asn Cys Ala Ser Val Cys Asn Leu Glu Gly Phe Ser

20 25 30

Gly Gly Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His

35 40 45

Cys

<210> 182

<211> 47

<212> PRT

<213> Peanut (Arachis hypogaea)

<400> 182

Arg Val Cys Gln Ser Lys Ser His Met Phe Lys Gly Gly Cys Trp Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 183

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 183

Arg Thr Cys Glu Thr Ser Ser Asn Leu Phe Asn Gly Pro Cys Leu Ser

1 5 10 15

Ser Ser Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Ser Asp Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Leu Cys Thr Arg Pro Cys

35 40 45

<210> 184

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 184

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ala Ser Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Val Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 185

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 185

Arg Thr Cys Ala Ser Gln Ser Gln Arg Phe Lys Gly Lys Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Glu Asn Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 186

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 186

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Thr His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 187

<211> 45

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 187

Arg Thr Cys Glu Ser Pro Ser Asn Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ser Gln Ser Cys Ala Lys Ala Cys Pro Ser Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Ser Ser Leu Arg Cys Tyr Cys Ser Lys Ala Cys

35 40 45

<210> 188

<211> 57

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 188

Glu Val Ser Pro Leu Asp Asn Lys Ile Cys Lys Thr Arg Ser Asp Arg

1 5 10 15

Phe Ser Gly Val Cys Ile Ser Thr Asn Asn Cys Ala Ile Ile Cys Gln

20 25 30

Gln Phe Glu His Phe Asp Gly Gly His Cys Glu Phe Asp Gly Ala Phe

35 40 45

Arg Arg Cys Met Cys Thr Lys Gln Cys

50 55

<210> 189

<211> 57

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 189

Gln Pro Gln Leu Cys Glu Thr Lys Ser Leu Asn Tyr Arg Gly Leu Cys

1 5 10 15

Leu Lys Trp Arg Ser Cys Lys Arg Val Cys Ile Ser Glu Gly Phe Pro

20 25 30

Asp Gly Arg Cys Lys Gly Phe Phe Asn Asn Lys Cys Val Cys Arg Lys

35 40 45

Pro Cys Ala Leu Leu Ser Thr Glu Asn

50 55

<210> 190

<211> 53

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 190

Gln Gln Met Cys Glu Ala Lys Ser Leu Asp Trp Lys Gly Met Cys Leu

1 5 10 15

Lys Trp Arg Asn Cys Arg Gln Val Cys Ile Ser Glu Gly Phe Thr Asp

20 25 30

Gly Arg Cys Lys Gly Phe Thr Arg Lys Cys Ile Cys Ser Lys Pro Cys

35 40 45

Phe Val Leu Pro Asn

50

<210> 191

<211> 47

<212> PRT

<213> Arabidopsis thaliana (Arabidopsis thaliana)

<400> 191

Arg Thr Cys Gln Ser Lys Ser His His Phe Lys Tyr Met Cys Thr Ser

1 5 10 15

Asn His Asn Cys Ala Ile Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys His Gly Phe His Arg Arg Cys Tyr Cys Thr Arg Leu Cys

35 40 45

<210> 192

<211> 58

<212> PRT

<213> Acer truncatum (Acer truncatum)

<400> 192

Glu Met Gly Gln Asn Gln Met Val Ala Glu Ala Arg Thr Cys Glu Ser

1 5 10 15

Gln Ser His Lys Phe Lys Gly Thr Cys Val Ser Lys Ser Asn Cys Ala

20 25 30

His Val Cys Gln Thr Glu Gly Phe Pro Gly Gly His Cys Arg Gly Phe

35 40 45

Arg Arg Arg Cys Phe Cys Ala Lys Asn Cys

50 55

<210> 193

<211> 55

<212> PRT

<213> Acer truncatum (Acer truncatum)

<400> 193

Gln Asn Glu Met Val Ala Glu Ala Arg Thr Cys Glu Ser Gln Ser His

1 5 10 15

Lys Phe Lys Gly Thr Cys Leu Ser Lys Ser Asn Cys Ala Asn Val Cys

20 25 30

Lys Thr Glu Gly Phe Pro Gly Gly His Cys Arg Gly Phe Arg Arg Arg

35 40 45

Cys Phe Cys Thr Lys Lys Cys

50 55

<210> 194

<211> 49

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 194

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys His Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 195

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 195

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Glu Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 196

<211> 75

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 196

Met Asp Pro Val Thr Val Glu Ala Arg Thr Cys Glu Ser Lys Ser His

1 5 10 15

Lys Phe Arg Gly Leu Cys Val Ser Thr His Asn Cys Ala Asn Val Cys

20 25 30

His Thr Lys Val Lys Arg Ser Lys Glu Cys Val Ser Thr Asp Arg Thr

35 40 45

Val Pro Leu Leu Val Pro Lys Lys Val Pro Gln Ala Val Val Ala Val

50 55 60

Val Ser Val Ala Pro Ala Pro Asn Pro Ala Ser

65 70 75

<210> 197

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 197

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Pro Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 198

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 198

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Thr His Cys

35 40 45

<210> 199

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 199

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Leu Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 200

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 200

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Thr Thr Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 201

<211> 47

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 201

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ser Thr Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 202

<211> 49

<212> PRT

<213> Egyoki mustard (Brassica carinata)

<400> 202

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys Lys Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 203

<211> 49

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 203

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys Lys Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 204

<211> 47

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 204

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 205

<211> 50

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 205

Ile Glu Gly Arg Met Cys Gln Ser Arg Ser His His Phe Arg Ser Ile

1 5 10 15

Cys Leu Ser Asn His Asn Cys Ala Asn Ile Cys Arg Gly Glu Arg Phe

20 25 30

Ser Gly Gly Arg Cys His Gly Leu His Arg Lys Cys Tyr Cys Thr Arg

35 40 45

Leu Cys

50

<210> 206

<211> 72

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 206

Gln Gln Met Cys Glu Ala Lys Ser Leu Asn Phe Lys Gly Met Cys Leu

1 5 10 15

Lys Trp Lys Asn Cys Lys His Val Phe Phe Thr Arg Lys Gln Leu Ile

20 25 30

Lys Leu Ser Lys Lys Asp Val Ala Ala Ala Glu Glu Glu Lys Ile Leu

35 40 45

Lys Glu Thr Cys Met Lys Leu Val Gln Gly Gln Gln Met Cys Glu Ala

50 55 60

Lys Ser Ile Asn Phe Lys Gly Met

65 70

<210> 207

<211> 47

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 207

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ser Thr Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 208

<211> 47

<212> PRT

<213> Cabbage (Brassica oleracea)

<400> 208

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Leu Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 209

<211> 49

<212> PRT

<213> Turnip (Brassica rapa)

<400> 209

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys Lys Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 210

<211> 47

<212> PRT

<213> Turnip (Brassica rapa)

<400> 210

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 211

<211> 47

<212> PRT

<213> Turnip (Brassica rapa)

<400> 211

Arg Met Cys Gln Ser Arg Ser His His Phe Arg Ser Ile Cys Leu Ser

1 5 10 15

Asn His Asn Cys Ala Asn Ile Cys Arg Gly Glu Arg Phe Ser Gly Gly

20 25 30

Arg Cys His Gly Leu His Arg Lys Cys Tyr Cys Thr Arg Leu Cys

35 40 45

<210> 212

<211> 47

<212> PRT

<213> Turnip (Brassica rapa)

<400> 212

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ser Thr Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 213

<211> 47

<212> PRT

<213> Turnip (Brassica rapa)

<400> 213

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Pro Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 214

<211> 49

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 214

Lys Ala Arg Met Cys Glu Thr Ser Ser Gln Leu Phe Asn Gly Pro Cys

1 5 10 15

Leu Ser Thr Thr Asn Cys Ala Asn Ile Cys Gln Asn Glu Gly Phe Pro

20 25 30

Asp Gly Asp Cys Lys Gly Phe Arg Leu Arg Cys Ile Cys Asn Arg Pro

35 40 45

Cys

<210> 215

<211> 47

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 215

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Asn Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 216

<211> 50

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 216

Ile Glu Gly Arg Met Cys Gln Ser Arg Ser His His Phe Arg Ser Ile

1 5 10 15

Cys Leu Ser Asn His Asn Cys Ala Asn Ile Cys Arg Gly Glu Arg Phe

20 25 30

Ser Gly Gly Arg Cys His Gly Leu His Arg Lys Cys Tyr Cys Thr Arg

35 40 45

Leu Cys

50

<210> 217

<211> 72

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 217

Gln Gln Met Cys Glu Ala Lys Ser Ile Asn Phe Lys Gly Met Ser Leu

1 5 10 15

Lys Trp Lys Asn Cys Lys His Phe Ile His Glu Asn Glu Leu Ile Lys

20 25 30

Leu Ser Arg Lys Glu Val Ala Ala Ala Glu Glu Glu Lys Phe Leu Lys

35 40 45

Glu Thr Cys Met Lys Leu Val Gln Gly Gln Gln Met Cys Glu Ala Lys

50 55 60

Ser Ile Asn Phe Lys Gly Ile Cys

65 70

<210> 218

<211> 47

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 218

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ser Thr Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 219

<211> 45

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 219

Arg Thr Cys Glu Ser Pro Ser Thr Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ala Lys Asn Cys Ala Gly Val Cys Thr Gly Glu Gly Phe Thr Gly Gly

20 25 30

Gln Cys Ser Ser Leu Lys Cys Tyr Cys Thr Lys Thr Cys

35 40 45

<210> 220

<211> 47

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 220

Arg Thr Cys Glu Ser Lys Ser His Lys Phe Arg Gly Leu Cys Val Ser

1 5 10 15

Arg His Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe His Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 221

<211> 48

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 221

Arg Ser Cys Glu Ser Ala Ser Gln Arg Phe Asn Gly Tyr Cys Val Arg

1 5 10 15

Ser Ser Asn Cys Ala Ser Ile Cys Ser Thr Glu Gly Phe Thr Asp Gly

20 25 30

Lys Cys Lys Gly Thr Ile Arg Arg His Cys Thr Cys Ile Arg Asn Cys

35 40 45

<210> 222

<211> 48

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 222

Arg Val Cys Ile Ser Gln Ser His Gly Phe Lys Gly Pro Cys Gly His

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Ile Gly Val Phe Ile Arg Lys Cys Tyr Cys Thr Lys Asn Cys

35 40 45

<210> 223

<211> 48

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 223

Lys Ile Cys Glu Ala Leu Ser Gly Asn Phe Lys Gly Leu Cys Leu Ser

1 5 10 15

Ser Arg Asp Cys Gly Asn Val Cys Arg Arg Glu Gly Phe Thr Asp Gly

20 25 30

Ser Cys Ile Gly Phe Arg Leu Gln Cys Phe Cys Thr Lys Pro Cys Ala

35 40 45

<210> 224

<211> 47

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 224

Arg Lys Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 225

<211> 47

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 225

Arg His Cys Glu Ser Lys Ser Gln Arg Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Gln Lys Asn Cys Ala Ala Val Cys Glu Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 226

<211> 47

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 226

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Val Cys Ala Ser

1 5 10 15

Glu Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 227

<211> 51

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 227

Lys Thr Cys Asp Thr Leu Ser Glu Asn Phe Lys Gly Ile Cys Phe Ser

1 5 10 15

Ser Thr Asn Cys Ala Thr Thr Cys Gln Asn Glu Asp Glu Glu Tyr Thr

20 25 30

Gly Gly His Cys Arg Gly Leu His Arg Arg Cys Phe Cys Thr Thr Glu

35 40 45

Cys Glu Leu

50

<210> 228

<211> 49

<212> PRT

<213> Capsici fructus (Capsicum annuum)

<400> 228

Lys Thr Cys Glu Arg Leu Ser Gly Thr Phe Lys Gly Ile Cys Phe Ser

1 5 10 15

Ser Lys Asn Cys Ala Ser Val Cys Glu Asn Glu Asp Glu Glu Phe Thr

20 25 30

Gly Gly His Cys Ser Gly Leu Arg Arg Arg Cys Tyr Cys Thr Ala Asp

35 40 45

Cys

<210> 229

<211> 47

<212> PRT

<213> Hemp (Cannabis sativa)

<400> 229

Arg Val Cys Glu Ser Lys Ser His His Phe Lys Gly Leu Cys Val Ile

1 5 10 15

Asp His Asn Cys Ala Thr Ile Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Gln Gly Phe Arg Gln Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 230

<211> 47

<212> PRT

<213> Hemp (Cannabis sativa)

<400> 230

Arg Thr Cys Glu Ala Gln Ser His His Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Asn Val Cys Arg Thr Glu Gly Phe Pro Ser Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 231

<211> 47

<212> PRT

<213> Hemp (Cannabis sativa)

<400> 231

Lys Thr Cys Glu Ala Arg Ser Gln Ser His Arg Gly Ile Cys Val Thr

1 5 10 15

Asn His Thr Cys Asp Asn Val Cys Lys Ile Glu Arg Phe Ser Ser Gly

20 25 30

Tyr Cys Ser Gly Ile Asp Arg Arg Cys Leu Cys Thr Lys His Cys

35 40 45

<210> 232

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 232

Arg Thr Cys Gln Ser Ser Ser Gln Leu Phe Ser Gly Pro Arg Leu Ser

1 5 10 15

Thr Ser Asn Cys Ala Asn Val Cys Gln Asn Glu Gly Phe Thr Asp Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Asn Lys Gln Cys

35 40 45

<210> 233

<211> 54

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 233

Gln Gln Leu Cys Glu Thr Lys Ser Thr Asn Tyr Arg Gly Leu Cys Leu

1 5 10 15

Arg Trp Arg Asn Cys Lys Arg Val Cys Met Ser Glu Gly Phe Pro Asp

20 25 30

Gly Arg Cys Arg Gly Ile Phe Asp Asn Lys Cys Val Cys Arg Lys Pro

35 40 45

Cys Ala Leu Ser Thr Ala

50

<210> 234

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 234

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Ala Ser Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Asn Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 235

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 235

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Thr Asn

1 5 10 15

Glu Gln Asn Cys Arg Asn Ile Cys His Asn Glu Gly Phe Phe Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 236

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 236

Arg Met Cys Glu Ser Lys Ser Gln Gln Phe Arg Gly Leu Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Thr Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Glu Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 237

<211> 100

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 237

Lys Pro Arg Arg Cys Glu Ser Gln Ser Asn Lys Phe Lys Gly Lys Cys

1 5 10 15

Val Ser Glu Thr Asn Cys Gly Asn Val Cys Arg Thr Glu Gly Phe Thr

20 25 30

Gly Gly Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn

35 40 45

Cys Cys Ala Arg His Arg Met Lys Leu Ser Met Arg Leu Ile Ser Ala

50 55 60

Ala Leu Leu Leu Leu Met Leu Phe Val Ala Thr Gly Met Gly Pro Val

65 70 75 80

Met Val Asp Ala Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly

85 90 95

Pro Cys Val Ser

100

<210> 238

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 238

Arg Val Cys Glu Thr Arg Ser Gln Lys Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Asn Asn Cys Ala Asn Val Cys Gln Thr Glu Gly Phe Pro Asp Gly

20 25 30

Asp Cys Asn Gly Ile Leu Arg Arg Cys Tyr Cys Asn Ser Pro Cys

35 40 45

<210> 239

<211> 45

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 239

Lys Thr Cys Glu Thr Pro Ser Thr Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ser Gln Asn Cys Ala Lys Val Cys Pro Thr Glu Gly Ala Ser Gly Gly

20 25 30

Arg Cys Asn Ser Leu Arg Cys Val Cys Ser Lys Thr Cys

35 40 45

<210> 240

<211> 47

<212> PRT

<213> Cardamine (CARDAMINE HIRSUTA)

<400> 240

Arg Ile Cys Gln Ser Lys Ser His His Tyr Lys Tyr Met Cys Thr Ser

1 5 10 15

Asn His Asn Cys Ala Met Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Gln Cys His Gly Phe His Arg Arg Cys Tyr Cys Thr Arg Leu Cys

35 40 45

<210> 241

<211> 47

<212> PRT

<213> Light red camelina sativa (Capsella rubela)

<400> 241

Arg Thr Cys Glu Ser Ser Ser Asn Leu Phe Ser Gly Pro Cys Leu Ser

1 5 10 15

Glu Ser Asn Cys Ala Asn Val Cys Asn Thr Glu Gly Phe Pro Asp Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Met Cys Thr Arg Pro Cys

35 40 45

<210> 242

<211> 47

<212> PRT

<213> Light red camelina sativa (Capsella rubela)

<400> 242

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Gly Asn Asn Cys Lys Asn Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 243

<211> 45

<212> PRT

<213> Light red camelina sativa (Capsella rubela)

<400> 243

Arg Thr Cys Glu Ser Pro Ser Ser Lys Phe Ser Gly Val Cys Leu Asn

1 5 10 15

Ser Gln Thr Cys Ala Lys Ala Cys Pro Ser Glu Gly Phe Thr Gly Gly

20 25 30

Arg Cys Asn Ser Leu Arg Cys Tyr Cys Ser Lys Pro Cys

35 40 45

<210> 244

<211> 57

<212> PRT

<213> Light red camelina sativa (Capsella rubela)

<400> 244

Glu Met Gly Leu Ala Ala Asp Lys Val Cys Lys Thr Arg Ser Asp Arg

1 5 10 15

Phe Ser Gly Val Cys Leu Ser Asn Asn Asn Cys Ala Ile Ile Cys Gln

20 25 30

Gln Phe Glu Asn Phe Asp Ser Gly His Cys Glu Phe Asp Gly Ala Phe

35 40 45

Arg Arg Cys Leu Cys Thr Lys Asn Cys

50 55

<210> 245

<211> 47

<212> PRT

<213> Light red camelina sativa (Capsella rubela)

<400> 245

Arg Met Cys Gln Ser Lys Ser His His Phe Arg Arg Met Cys Trp Ser

1 5 10 15

Asp His Asn Cys Ala Met Ile Cys Arg Ser Glu Arg Phe Ser Gly Gly

20 25 30

His Cys His Gly Phe His Arg Arg Cys Tyr Cys Thr Arg Leu Cys

35 40 45

<210> 246

<211> 47

<212> PRT

<213> Chickpea (Cicer arientinum)

<400> 246

Arg Val Cys Ala Ser Gln Ser His Ala Phe Lys Gly Pro Cys Val Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Gln Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 247

<211> 47

<212> PRT

<213> Clay citrus (Citrus clementina)

<400> 247

Arg Thr Cys Glu Ser Leu Ser His Arg Phe Lys Gly Thr Cys Val Arg

1 5 10 15

Gly Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Gln Cys Ser Gly Leu Arg Arg Arg Cys Phe Cys Ser Lys Pro Cys

35 40 45

<210> 248

<211> 77

<212> PRT

<213> Clay citrus (Citrus clementina)

<400> 248

His Asp Thr Glu Met Gly Pro Val Leu Cys Gly Asp Thr Gly Asn Glu

1 5 10 15

Lys Gly Pro Ser Arg Arg Lys Ala Ala Ala Val Ala Ala Ile Glu Arg

20 25 30

Thr Cys Glu Thr Pro Ser Lys Arg Phe Lys Gly Pro Cys Val Lys Glu

35 40 45

Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Gln Gly Gly Lys

50 55 60

Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Pro

65 70 75

<210> 249

<211> 47

<212> PRT

<213> Clay citrus (Citrus clementina)

<400> 249

Arg Ile Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Ile Arg

1 5 10 15

Lys Asp Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 250

<211> 47

<212> PRT

<213> Goldfish algae (Ceratophyllum demensis)

<400> 250

Arg Met Cys Glu Ser Pro Ser Arg His Tyr Lys Gly Leu Cys Phe Arg

1 5 10 15

Ser Ser Asn Cys Ala Ser Ile Cys His Ile Glu His Phe Ser His Gly

20 25 30

His Cys His Gly Phe Arg Arg Arg Cys Ile Cys Ser Arg Pro Cys

35 40 45

<210> 251

<211> 47

<212> PRT

<213> Goldfish algae (Ceratophyllum demensis)

<400> 251

Arg Met Cys Glu Ser Pro Ser Arg His Tyr Lys Gly Leu Cys Phe Arg

1 5 10 15

Ser Ser Asn Cys Ala Ser Ile Cys His Ile Glu His Phe Ser His Gly

20 25 30

Tyr Cys His Gly Phe Arg Arg Arg Cys Ile Cys Ser Arg Pro Cys

35 40 45

<210> 252

<211> 47

<212> PRT

<213> Qian Chuan Qian elm (Carpinus fangiana)

<400> 252

Arg Val Cys Glu Ser Gln Ser His Lys Phe His Gly Pro Cys Leu Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Lys Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 253

<211> 50

<212> PRT

<213> Watermelon (Citrullus lanatus)

<400> 253

Ala Glu Ala Arg Ile Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro

1 5 10 15

Cys Val Ser Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe

20 25 30

His Gly Gly His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys

35 40 45

Arg Cys

50

<210> 254

<211> 47

<212> PRT

<213> Watermelon (Citrullus lanatus)

<400> 254

Arg Val Cys Gln Ser Gln Ser His His Phe His Gly Ala Cys Phe Ser

1 5 10 15

His His Asn Cys Ala Phe Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Ala Arg Arg Arg Cys Phe Cys Ser Lys Leu Cys

35 40 45

<210> 255

<211> 47

<212> PRT

<213> Illinois hickory nut (Carya illinoinensis)

<400> 255

Arg Val Cys Glu Ser Lys Ser His Gly Phe Gln Gly Met Cys Thr Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 256

<211> 47

<212> PRT

<213> Illinois hickory nut (Carya illinoinensis)

<400> 256

Arg Val Cys Glu Ser Lys Ser His Lys Phe Gln Gly Pro Cys Val Arg

1 5 10 15

Asp Asn Asn Cys Gly Leu Val Cys Lys Asn Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Ile Cys

35 40 45

<210> 257

<211> 58

<212> PRT

<213> Watermelon (Citrullus lanatus)

<400> 257

Glu Glu Met Met Gly Gly Gly Arg Ala Glu Gly Arg Val Cys Lys Ser

1 5 10 15

Gln Ser His Ser Phe His Gly Pro Cys Met Arg Asp His Asn Cys Ala

20 25 30

Val Val Cys Arg Thr Glu Gly Phe Ser Gly Gly Asp Cys Val Gly Phe

35 40 45

Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

50 55

<210> 258

<211> 47

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 258

Arg Val Cys Glu Ser Lys Ser His Trp Phe Lys Gly Pro Cys Trp Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 259

<211> 47

<212> PRT

<213> Corchorus olitorius (Corchorus olitorius)

<400> 259

Arg Leu Cys Gln Ser Gln Ser His Gly Phe Lys Gly Gly Cys Leu Ser

1 5 10 15

Asn His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Gln Cys Arg Gly Leu Arg His Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 260

<211> 32

<212> PRT

<213> Papaya (CARICA PAPAYA)

<220>

<221> Feature not yet classified

<222> (32)..(32)

<223> Xaa can be any naturally occurring amino acid

<400> 260

Arg Thr Cys Gln Ser Gln Ser His Gly Phe His Gly Ala Cys Leu Ser

1 5 10 15

His His Asn Cys Ala Val Val Cys Gln Lys Glu Gly Phe Ser Gly Xaa

20 25 30

<210> 261

<211> 47

<212> PRT

<213> Papaya (CARICA PAPAYA)

<400> 261

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Ala Cys Gly Ser

1 5 10 15

Arg Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 262

<211> 50

<212> PRT

<213> Cucumber (culumis sativus)

<400> 262

Val Glu Gly Arg Ile Cys Glu Ser Lys Ser His Gly Phe Lys Gly Pro

1 5 10 15

Cys Met Thr Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe

20 25 30

Ser Gly Gly Lys Cys Arg Gly Val Arg His Arg Cys Phe Cys Thr Lys

35 40 45

Leu Cys

50

<210> 263

<211> 50

<212> PRT

<213> Cucumber (culumis sativus)

<400> 263

Val Glu Gly Arg Ile Cys Glu Ser Lys Ser His Gly Phe Lys Gly Pro

1 5 10 15

Cys Met Thr Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe

20 25 30

Ser Gly Gly Lys Cys Arg Gly Val Arg His Arg Cys Phe Cys Thr Lys

35 40 45

Leu Cys

50

<210> 264

<211> 73

<212> PRT

<213> Carrot (Daucus carota)

<400> 264

Thr Asp Thr Cys Glu Val Arg Ser Ala Tyr Phe Val Ser Leu Cys Leu

1 5 10 15

Ile Asp His His Cys Glu Met Val Cys Lys Val Glu Gly Phe Pro Thr

20 25 30

Gly Lys Cys Leu Gly Leu Val Pro His Cys Ile Cys Leu Gly Thr Cys

35 40 45

Lys Gln Pro Ser Pro Pro Ser Pro Pro Pro Ala Asn Pro Pro Glu Ser

50 55 60

Pro Pro Pro Val Pro Pro Pro Thr Pro

65 70

<210> 265

<211> 47

<212> PRT

<213> Carrot (Daucus carota)

<400> 265

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Leu Ser

1 5 10 15

Glu Thr Asn Cys Gly Asn Val Cys Lys Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 266

<211> 47

<212> PRT

<213> Carrot (Daucus carota)

<400> 266

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Arg Ser Asn Cys Ala Asn Val Cys Lys Asn Glu Gly Phe Pro Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Ile Lys His Cys

35 40 45

<210> 267

<211> 47

<212> PRT

<213> Davidia involucrata (Davidia involucrata)

<400> 267

Arg Val Cys Glu Ala Gln Ser His Gly Phe Lys Gly Ala Cys Met Ser

1 5 10 15

Asn His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Lys Gln Cys

35 40 45

<210> 268

<211> 47

<212> PRT

<213> Durian (Durio zibethin)

<400> 268

Arg Val Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Leu Ser

1 5 10 15

His His Asn Cys Gly Met Val Cys Lys Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Val Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 269

<211> 52

<212> PRT

<213> Durian (Durio zibethin)

<400> 269

Ile Ser Val Glu Gly Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys

1 5 10 15

Gly Ala Cys Val Ser Glu Asn Asn Cys Ala Ala Val Cys Gln Thr Glu

20 25 30

Gly Phe Leu Gly Gly Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys

35 40 45

Thr Lys Gln Cys

50

<210> 270

<211> 52

<212> PRT

<213> Durian (Durio zibethin)

<400> 270

Ile Pro Leu Glu Gly Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys

1 5 10 15

Gly Met Cys Val Arg Glu Ser Asn Cys Ala Thr Val Cys Gln Thr Glu

20 25 30

Gly Phe Gln Gly Gly His Cys Arg Gly Phe His Arg Arg Cys Phe Cys

35 40 45

Thr Lys His Cys

50

<210> 271

<211> 47

<212> PRT

<213> Durian (Durio zibethin)

<400> 271

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Leu Arg

1 5 10 15

Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 272

<211> 50

<212> PRT

<213> Durian (Durio zibethin)

<400> 272

Gly Glu Ala Arg Val Cys Glu Ser Lys Ser His Lys Tyr Lys Gly Ala

1 5 10 15

Cys Phe His Asp His Asn Cys Ala Tyr Val Cys Arg Asn Glu Gly Phe

20 25 30

Ser Gly Gly Lys Cys His Gly Leu His His His Cys Phe Cys Thr Lys

35 40 45

Ile Cys

50

<210> 273

<211> 47

<212> PRT

<213> Durian (Durio zibethin)

<400> 273

Arg Val Cys Glu Ser Lys Ser His Lys Phe Lys Gly Pro Cys Met Gly

1 5 10 15

Asp His Asn Cys Ala Tyr Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg His Arg Cys Phe Cys Thr Lys Ile Cys

35 40 45

<210> 274

<211> 47

<212> PRT

<213> Suaeda salsa (Erigeron canadensis)

<400> 274

Arg Met Cys Gln Ser Gln Ser His Met Tyr His Gly Ala Cys Leu Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 275

<211> 71

<212> PRT

<213> Suaeda salsa (Erigeron canadensis)

<400> 275

Asn Glu Met Gly Gly Pro Met Val Val Glu Gly Arg Thr Leu Ala Val

1 5 10 15

Val Thr Val Ile Gly Ala Gly Gly Val Gln Arg Trp Trp Arg Trp Trp

20 25 30

Gln Cys Asn Gly Asp Ser Gly Gly Asp Gly Gly Thr Arg Ala Met Val

35 40 45

Ala Val Thr Asn Ser Gly Gly Ser Ser Ser Asn Gly Gly Gly Gly Asp

50 55 60

Asp Trp Trp Trp Trp Trp Gln

65 70

<210> 276

<211> 48

<212> PRT

<213> Salt mustard (Eutrema salsugenium)

<400> 276

Arg Met Cys Thr Asn Pro Ser Lys Thr Tyr Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Glu Ser Val Cys Gln Ser Glu Gly Phe Pro Gln Gly

20 25 30

Asn Cys His Gly Phe Arg Arg Arg Cys Ile Cys Gly Lys Pro Cys Ala

35 40 45

<210> 277

<211> 47

<212> PRT

<213> Salt mustard (Eutrema salsugenium)

<400> 277

Arg Thr Cys Glu Thr Ala Ser His Lys Phe Lys Gly Pro Cys Leu Gly

1 5 10 15

Asp Arg Asn Cys Ala Asn Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asp Cys Gln Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 278

<211> 48

<212> PRT

<213> Salt mustard (Eutrema salsugenium)

<400> 278

Arg Thr Cys Asp Ala Pro Ser His Ser Phe Lys Gly Ile Cys Thr Arg

1 5 10 15

Lys Arg Asn Cys Glu Ser Val Cys Asn Ser Glu Gly Phe Ala Asp Gly

20 25 30

Asp Cys Lys Gly Phe Arg Arg Arg Cys Ile Cys Asn Arg Pro Cys Ala

35 40 45

<210> 279

<211> 49

<212> PRT

<213> Salt mustard (Eutrema salsugenium)

<400> 279

Arg Thr Cys Val Thr Pro Ser His Arg Phe Arg Gly Ile Cys Val Ser

1 5 10 15

Lys Arg Asn Cys Glu Ser Ala Cys Asn Ser Glu Gly Phe Pro Asp Gly

20 25 30

Asn Cys Lys Gly Phe Arg Arg Arg Cys Ile Cys Asn Arg Pro Cys Ala

35 40 45

Lys

<210> 280

<211> 51

<212> PRT

<213> Salt mustard (Eutrema salsugenium)

<400> 280

Leu Phe Gly Arg Cys Lys Lys Val Pro Ser Arg Thr Tyr Lys Gly Pro

1 5 10 15

Cys Ala Arg Asp Ala Asn Cys Arg Ser Val Cys Ile Ser Glu Gly Tyr

20 25 30

Asn Gly Gly Asp Cys His Gly Phe Arg Arg Arg Cys Met Cys Thr Asp

35 40 45

Lys Cys Ser

50

<210> 281

<211> 47

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 281

Arg Thr Cys Glu Ser Gln Ser Gln Arg Phe Lys Gly Ala Cys Val Ser

1 5 10 15

Lys Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 282

<211> 50

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 282

Ile Glu Gly Arg Met Cys Glu Ser Gln Ser His Lys Phe His Gly Leu

1 5 10 15

Cys Met Ser Asp Arg Thr Cys Val Val Val Cys Lys Glu Glu Asp Phe

20 25 30

Asp Asp Gly Lys Cys Arg Gly Phe Arg His Arg Cys Phe Cys Arg Lys

35 40 45

Pro Cys

50

<210> 283

<211> 47

<212> PRT

<213> Eucalyptus robusta (Eucalyptus grandis)

<400> 283

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Ser Cys Val Ser

1 5 10 15

Gly His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 284

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 284

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Thr Cys Ile Arg

1 5 10 15

Ala His Asn Cys Ala Leu Val Cys Arg His Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 285

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 285

Arg Thr Cys Glu Thr Leu Ser His Lys Phe Lys Gly Thr Cys Leu Arg

1 5 10 15

Glu Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 286

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 286

Arg Val Cys Glu Ser Gln Ser His Gly Phe Tyr Gly Thr Cys Ile His

1 5 10 15

Ala His Asn Cys Ala Leu Val Cys Arg His Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 287

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 287

Ala Ile Cys Lys Val Pro Ser Lys Thr Phe Lys Gly Met Cys Phe Ser

1 5 10 15

Gln Lys Asn Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Trp Gly

20 25 30

Glu Cys Ser His Leu Arg Arg Arg Cys Met Cys Tyr Lys Pro Cys

35 40 45

<210> 288

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 288

Arg Val Cys Glu Val Pro Ser Lys Thr Phe Thr Gly Leu Cys Phe Arg

1 5 10 15

Asp Thr His Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Tyr Gly

20 25 30

Lys Cys Ser His Val Leu Arg Arg Cys Arg Cys Leu Lys Ala Cys

35 40 45

<210> 289

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 289

Ala Ile Cys Lys Val Pro Ser Lys Thr Phe Lys Gly Met Cys Phe Ser

1 5 10 15

Gln Lys Asn Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Trp Gly

20 25 30

Glu Cys Ser His Leu Arg Arg Arg Cys Met Cys Tyr Lys Pro Cys

35 40 45

<210> 290

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 290

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Thr Cys Ile Arg

1 5 10 15

Ala His Asn Cys Ala Leu Val Cys Arg His Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 291

<211> 53

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 291

Gly Arg Pro Asp Pro Lys Thr Cys Glu Ser Pro Ser Ala Lys Phe Glu

1 5 10 15

Gly Pro Cys Ile Met Gly Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu

20 25 30

Gly His Asp Gly Gly Phe Cys Arg Asp Phe Trp Leu Pro Lys Cys Phe

35 40 45

Cys Thr Lys Pro Cys

50

<210> 292

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 292

Arg Thr Cys Glu Ser Leu Ser His Lys Phe Gln Gly Thr Cys Leu Arg

1 5 10 15

Glu Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 293

<211> 53

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 293

Gly Arg Pro Asp Pro Lys Thr Cys Glu Pro Pro Ser Ala Lys Phe Glu

1 5 10 15

Glu Pro Cys Ile Met Gly Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu

20 25 30

Gly His Asp Gly Gly Phe Cys His Asp Phe Trp Leu Pro Lys Cys Phe

35 40 45

Cys Thr Lys Pro Cys

50

<210> 294

<211> 75

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 294

Leu Gly Pro Lys Val Met Val Ala His Glu Ala Ile Thr Cys Glu Thr

1 5 10 15

Gln Ser Ile Lys Tyr Glu Gly Met Cys Ser Arg Glu Lys Pro Cys Ala

20 25 30

Ser Leu Cys Gln Thr Lys Gly Tyr Ser Arg Gly Glu Cys His Glu Val

35 40 45

Ile Thr Leu Arg Leu Leu Tyr Arg Pro Gly Lys Thr Asp Pro Ile Gly

50 55 60

Gln Ser Lys Arg Arg Thr Tyr Pro Leu Thr Asn

65 70 75

<210> 295

<211> 47

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 295

Arg Val Cys Glu Val Pro Ser Lys Thr Phe Thr Gly Leu Cys Phe Arg

1 5 10 15

Asp Thr His Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Tyr Gly

20 25 30

Lys Cys Ser His Val Leu Arg Arg Cys Arg Cys Leu Lys Ala Cys

35 40 45

<210> 296

<211> 53

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 296

Gly Arg Pro Asp Pro Lys Thr Cys Glu Ser Pro Ser Ala Lys Phe Glu

1 5 10 15

Gly Pro Cys Ile Met Gly Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu

20 25 30

Gly His Asn Gly Gly Phe Cys Arg Asp Phe Trp Leu Pro Lys Cys Phe

35 40 45

Cys Thr Lys Pro Cys

50

<210> 297

<211> 53

<212> PRT

<213> Modern strawberry cultivation (FRAGARAIA X ANANASSA)

<400> 297

Gly Arg Pro Asp Pro Lys Thr Cys Glu Ser Pro Ser Gly Lys Phe Glu

1 5 10 15

Gly Pro Cys Ile Met Gly Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu

20 25 30

Gly His Asp Gly Gly Phe Cys Arg Asp Phe Trp Leu Pro Lys Cys Phe

35 40 45

Cys Thr Lys Pro Cys

50

<210> 298

<211> 53

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 298

Gly Arg Pro Asp Pro Lys Thr Cys Glu Ser Pro Ser Ala Lys Phe Glu

1 5 10 15

Gly Pro Cys Ile Met Gly Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu

20 25 30

Gly His Asp Gly Gly Phe Cys Arg Asp Phe Trp Leu Pro Lys Cys Phe

35 40 45

Cys Thr Lys Pro Cys

50

<210> 299

<211> 58

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 299

Leu Gly Pro Lys Val Met Val Ala His Glu Ala Ile Thr Cys Glu Thr

1 5 10 15

Gln Ser Ile Lys Tyr Glu Gly Met Cys Ser Arg Glu Lys Pro Cys Ala

20 25 30

Ser Leu Cys Gln Thr Lys Gly Tyr Ser Arg Gly Glu Cys His Gly Val

35 40 45

Tyr Lys Ile Cys Val Cys Ala Thr Gln Cys

50 55

<210> 300

<211> 47

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 300

Ala Ile Cys Lys Val Pro Ser Lys Thr Phe Lys Gly Met Cys Phe Ser

1 5 10 15

Gln Lys Asn Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Trp Gly

20 25 30

Glu Cys Ser His Leu Arg Arg Arg Cys Met Cys Tyr Lys Pro Cys

35 40 45

<210> 301

<211> 48

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 301

Ser Ile Cys Glu Ile Pro Ser Lys Lys Phe Lys Gly Leu Cys Ile Arg

1 5 10 15

Asp Arg Lys Cys Ile Val Ile Cys His Ser Glu Gly Tyr Gly Tyr Gly

20 25 30

Lys Cys Ser His Ile Gly Arg Arg Cys Met Cys Leu Lys Thr Cys Asp

35 40 45

<210> 302

<211> 54

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 302

Met Val Ala Ala Asp Asp Ala Arg Lys Cys Glu Ser Leu Ser His Asn

1 5 10 15

Phe Asn Gly Leu Cys Leu Ser Glu Ser Asn Cys Ala Ser Val Cys Ala

20 25 30

Thr Glu Gly Phe Thr Gly Gly Asp Cys Arg Gly Leu Arg Arg Arg Cys

35 40 45

Phe Cys Thr Lys Gln Cys

50

<210> 303

<211> 47

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 303

Arg Thr Cys Glu Thr Leu Ser His Lys Phe Lys Gly Thr Cys Leu Arg

1 5 10 15

Glu Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 304

<211> 47

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 304

Arg Thr Cys Glu Ser Leu Ser His Lys Phe Lys Gly Thr Cys Leu Ser

1 5 10 15

Glu Thr Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 305

<211> 47

<212> PRT

<213> Wild strawberry (FRAGARAIA VESCA)

<400> 305

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Thr Cys Ile Arg

1 5 10 15

Ala His Asn Cys Ala Leu Val Cys Arg His Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 306

<211> 45

<212> PRT

<213> Soybean (Glycine max)

<400> 306

Met His Leu Arg Ser His Arg Phe Lys Gly Met Cys Leu Ser Glu His

1 5 10 15

Asn Cys Ala Ser Val Ser His Leu Glu Gly Phe Thr Gly Gly Lys Cys

20 25 30

Trp Gly Phe Arg Arg Arg Cys Phe Cys Ser Lys His Cys

35 40 45

<210> 307

<211> 47

<212> PRT

<213> Soybean (Glycine max)

<400> 307

Lys Asp Cys Leu Thr Arg Arg His Gly Phe Gln Gly Arg Cys Leu Phe

1 5 10 15

Asp Arg Gln Cys Ala His Val Cys Arg Ser Asp Gly Phe Ile Gly Gly

20 25 30

Gln Cys Arg Gly Pro Leu Arg Lys Cys Phe Cys Ser Arg Pro Cys

35 40 45

<210> 308

<211> 47

<212> PRT

<213> Soybean (Glycine max)

<400> 308

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Leu Cys Asn Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Ile Cys

35 40 45

<210> 309

<211> 47

<212> PRT

<213> Soybean (Glycine max)

<400> 309

Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Met Cys Leu Ser

1 5 10 15

Lys His Asn Cys Ala Ser Val Cys His Leu Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 310

<211> 63

<212> PRT

<213> Soybean (Glycine max)

<400> 310

Arg Tyr Ile Tyr Ile Ile Pro Tyr Val Ala Val Lys Arg Ala Glu Gly

1 5 10 15

Arg Asp Cys Ser Ser Lys Ser His Gly Phe Lys Gly Leu Cys Phe Ser

20 25 30

Asp Thr Asn Cys Ala His Val Cys Arg Thr Glu Gly Phe Thr Gly Gly

35 40 45

Asp Cys Gly Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Ile Cys

50 55 60

<210> 311

<211> 69

<212> PRT

<213> Soybean (Glycine max)

<400> 311

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Gly Ser Val Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Ser Arg Arg Cys Phe Cys Thr Lys Glu Tyr Asp

35 40 45

Asn Tyr Tyr Lys Ser Ser Asp Val Ile Arg Cys Lys Asp Gly Ser Gly

50 55 60

Lys Phe Thr Lys Ala

65

<210> 312

<211> 47

<212> PRT

<213> Soybean (Glycine max)

<400> 312

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Gly Ser Val Cys Arg Thr Glu Arg Phe Thr Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 313

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 313

Gly Pro Ile Cys Lys Ala Thr Ser Asn Val Tyr Lys Gly Leu Cys Leu

1 5 10 15

Ile Asp Asp Ser Cys Asp Ile Val Cys Lys Arg Glu Gly Phe His Lys

20 25 30

Gly Asn Cys Lys Gly Phe Leu Arg Lys Cys Val Cys Thr Asn Pro Cys

35 40 45

<210> 314

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 314

Lys Thr Cys Glu Thr Lys Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys Arg Gly Phe Arg Arg Gln Cys Val Cys Thr Lys Pro Cys

35 40 45

<210> 315

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 315

Thr Thr Cys Glu Thr Gln Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly Tyr Cys Arg Gly Phe Arg Arg Gln Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 316

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 316

Arg Thr Cys Glu Thr Lys Ser Ser Glu Tyr Lys Gly Ile Cys Leu Phe

1 5 10 15

Asp Ala Asn Cys Asp Ser Ile Cys Lys Val Glu Pro Ser Phe Asp Gly

20 25 30

Gly His Cys His Gly Phe Phe Arg Arg Cys Tyr Cys Thr Lys Pro Cys

35 40 45

<210> 317

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 317

Arg Thr Cys Glu Thr Lys Ser Ser Glu Tyr Lys Gly Ile Cys Leu Phe

1 5 10 15

Asp Ala Asn Cys Asp Ser Val Cys Lys Val Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys His Gly Phe Phe Arg Arg Cys Tyr Cys Thr Lys Pro Ser

35 40 45

<210> 318

<211> 51

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 318

Met Glu Asp Arg Ile Cys Gln Ser Arg Ser Thr Glu Tyr Arg Gly Met

1 5 10 15

Cys Leu Phe Asp Val Asn Cys Asp Asn Ile Cys Lys Thr Glu Pro Gly

20 25 30

Phe Thr Gly Gly His Cys His Ser Phe Phe Arg Ser Cys Tyr Cys Thr

35 40 45

Lys Pro Cys

50

<210> 319

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 319

Arg Lys Cys Glu Ser Pro Ser His Lys Phe Lys Gly Met Cys Met Asn

1 5 10 15

Arg Asp Asn Cys Ala Ile Val Cys Gln Thr Glu Gly Tyr Glu Asp Gly

20 25 30

Lys Cys Glu Gly Phe Arg Arg Arg Cys Ile Cys Ile Lys Pro Cys Phe

35 40 45

<210> 320

<211> 55

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 320

Gly Pro Thr Ile Val Gly Gly Arg Lys Cys Asp Ser Pro Ser Lys Lys

1 5 10 15

Phe Asn Gly Met Cys Met Asn His Asp Asn Cys Ala Thr Val Cys Gln

20 25 30

Thr Glu Ser His Glu Asp Gly Lys Cys Glu Gly Phe Tyr Arg Arg Cys

35 40 45

Ile Cys Ile Lys Pro Cys Phe

50 55

<210> 321

<211> 52

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 321

Gly Val Val Glu Gly Arg Ile Cys Glu Ser Lys Ser His Arg Phe Lys

1 5 10 15

Gly Val Cys Leu Ser Asp His Asn Cys Gly Leu Val Cys Arg Asn Glu

20 25 30

Gly Phe Leu Asp Gly Trp Cys Arg Gly Phe Arg His Arg Cys Phe Cys

35 40 45

Thr Arg Asn Cys

50

<210> 322

<211> 47

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 322

Arg Val Cys Gln Ser Gln Ser His Lys Phe His Gly Ala Cys Val Ser

1 5 10 15

Ser His Asn Cys Ala Met Val Cys Arg Asp Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 323

<211> 50

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 323

Asp Gly Asp Lys Ile Cys Glu Ser Pro Ser Asn Ala Phe Lys Gly Leu

1 5 10 15

Cys Leu Arg Asp Asp Asn Cys Asp Ile Val Cys Lys Thr Glu Gly Phe

20 25 30

Pro Asn Gly Asp Cys Lys Gly Phe Leu Arg Lys Cys Val Cys Thr Lys

35 40 45

Pro Cys

50

<210> 324

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 324

Lys Thr Cys Glu Thr Gln Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys Gln Gly Phe Arg Arg Gln Cys Val Cys Thr Lys Pro Cys

35 40 45

<210> 325

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 325

Lys Thr Cys Val Ala Glu Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys Gln Gly Phe Arg Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

<210> 326

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 326

Arg Thr Cys Glu Thr Lys Ser Ser Glu Tyr Lys Gly Ile Cys Leu Phe

1 5 10 15

Asp Ala Asn Cys Asp Ser Ile Cys Lys Val Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys His Gly Phe Phe Arg Arg Cys Tyr Cys Thr Lys Pro Cys

35 40 45

<210> 327

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 327

Lys Thr Cys Val Ala Glu Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Asp Phe Asp Gly

20 25 30

Gly His Cys Gln Gly Phe Arg Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

<210> 328

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 328

Arg Thr Cys Glu Thr Lys Ser Ser Glu Tyr Lys Gly Ile Cys Leu Phe

1 5 10 15

Asp Ala Asn Cys Asp Ser Ile Cys Lys Ile Glu Ser Gly Phe Asp Gly

20 25 30

Gly His Cys His Gly Phe Phe Arg Arg Cys Tyr Cys Thr Lys Pro Cys

35 40 45

<210> 329

<211> 65

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 329

Asp Glu Glu His Lys Glu Ile Glu Ile Gly Thr Met Ala Glu Glu Glu

1 5 10 15

His Lys Ile Cys Glu Ser Lys Ser Gly Lys Phe Lys Gly Met Cys Phe

20 25 30

Leu Asp Ala Asn Cys Asp Ser Ile Cys Lys Ala Glu Pro Gly Ser Thr

35 40 45

Gly Gly His Cys His Gly Ile Phe Arg Ile Cys Tyr Cys Thr Lys Pro

50 55 60

Cys

65

<210> 330

<211> 48

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 330

Arg Lys Cys Glu Ser Pro Ser His Lys Phe Lys Gly Met Cys Met Asn

1 5 10 15

Arg Asp Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Tyr Glu Asp Gly

20 25 30

Lys Cys Glu Gly Phe Arg Arg Arg Cys Ile Cys Ile Lys Pro Cys Phe

35 40 45

<210> 331

<211> 52

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 331

Gly Met Val Glu Gly Arg Ile Cys Glu Ser Lys Ser His Arg Phe Lys

1 5 10 15

Gly Val Cys Leu Ser Asp His Asn Cys Gly Leu Val Cys Arg Asn Glu

20 25 30

Gly Phe Leu Asp Gly Trp Cys Arg Gly Phe Arg His Arg Cys Phe Cys

35 40 45

Thr Arg Asn Cys

50

<210> 332

<211> 47

<212> PRT

<213> Upland cotton (Gossypium hirsutum)

<400> 332

Arg Val Cys Gln Ser Gln Ser His Lys Phe His Gly Ala Cys Leu Ser

1 5 10 15

Ser His Asn Cys Ala Met Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 333

<211> 50

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 333

Asp Gly Asp Lys Ile Cys Glu Ser Arg Ser Asn Ala Phe Lys Gly Leu

1 5 10 15

Cys Leu Arg Asp Asn Asn Cys Asp Ile Val Cys Lys Thr Glu Gly Phe

20 25 30

Pro Asn Gly Gly Cys Lys Gly Phe Ile Arg Lys Cys Val Cys Thr Lys

35 40 45

Pro Cys

50

<210> 334

<211> 48

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 334

Gly Thr Thr Cys Lys Ala Thr Ser Asn Ile Tyr Lys Gly Leu Cys Leu

1 5 10 15

Leu Asp Asp Ser Cys Asp Ile Val Cys Lys Arg Glu Gly Phe His Ser

20 25 30

Gly Asn Cys Lys Gly Phe Leu Arg Lys Cys Val Cys Thr Asn Pro Cys

35 40 45

<210> 335

<211> 50

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 335

Asp Gly Asp Lys Ile Cys Glu Ser Pro Ser Asn Ala Phe Lys Gly Leu

1 5 10 15

Cys Leu Arg Asp Asp Asn Cys Asp Ile Val Cys Lys Thr Glu Gly Phe

20 25 30

Pro Asn Gly Asp Cys Lys Gly Phe Leu Arg Lys Cys Val Cys Thr Lys

35 40 45

Pro Cys

50

<210> 336

<211> 48

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 336

Lys Thr Cys Glu Val Glu Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys Gln Gly Phe Arg Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

<210> 337

<211> 48

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 337

Lys Thr Cys Glu Val Glu Ser Gly Lys Phe Lys Gly Met Cys Met Ser

1 5 10 15

Ser Thr Asn Cys Ala Ser Val Cys Lys Ser Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys Gln Gly Phe Arg Arg Arg Cys Leu Cys Thr Lys Pro Cys

35 40 45

<210> 338

<211> 48

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 338

Arg Thr Cys Glu Thr Lys Ser Ser Glu Tyr Lys Gly Ile Cys Leu Phe

1 5 10 15

Asp Ala Asn Cys Asp Ser Ile Cys Lys Ile Glu Pro Gly Phe Asp Gly

20 25 30

Gly His Cys His Gly Phe Phe Arg Arg Cys Tyr Cys Thr Lys Pro Cys

35 40 45

<210> 339

<211> 48

<212> PRT

<213> Lei Mengshi Cotton (Gossypium raimondii)

<400> 339

Arg Lys Cys Glu Ser Pro Ser His Lys Phe Lys Gly Met Cys Met Asn

1 5 10 15

Arg Asp Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Tyr Glu Asp Gly

20 25 30

Lys Cys Glu Gly Phe Arg Arg Arg Cys Ile Cys Ile Lys Pro Cys Phe

35 40 45

<210> 340

<211> 47

<212> PRT

<213> European grape (VITIS VINIFERA)

<400> 340

Arg Val Cys Glu Ser Gln Ser His Lys Phe Glu Gly Ala Cys Met Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Lys Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 341

<211> 47

<212> PRT

<213> European grape (VITIS VINIFERA)

<400> 341

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Thr Cys Val Arg

1 5 10 15

Gln Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 342

<211> 47

<212> PRT

<213> Sunflower (Helianthus annuus)

<400> 342

Arg Ile Cys Glu Ser Pro Ser His Lys Phe Lys Gly Ile Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ser Ser Val Cys Gln Ser Glu Gly Phe Pro Trp Gly

20 25 30

Lys Cys Ile Gly Phe Arg Arg Arg Cys Phe Cys Leu Arg Glu Cys

35 40 45

<210> 343

<211> 47

<212> PRT

<213> Sunflower (Helianthus annuus)

<400> 343

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Lys Gly Thr Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ala Asn Val Cys His Ser Glu Arg Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys

35 40 45

<210> 344

<211> 47

<212> PRT

<213> Sunflower (Helianthus annuus)

<400> 344

Arg Met Cys Gln Ser Gln Ser His Met Phe His Gly Thr Cys Met Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 345

<211> 47

<212> PRT

<213> Sunflower (Helianthus annuus)

<400> 345

Arg Ile Cys Glu Ser Gln Ser Gln Glu Phe His Gly Ser Cys Met Ser

1 5 10 15

Asn His Asn Cys Gly Leu Val Cys Arg Thr Glu Gly Phe Ser Ala Gly

20 25 30

Ile Cys Arg Gly Val Arg Gly Arg Cys Phe Cys Leu Lys Thr Cys

35 40 45

<210> 346

<211> 47

<212> PRT

<213> Hydrangea (HYDRANGEA MACROPHYLLA)

<400> 346

Arg Thr Cys Glu Ser Lys Ser Asn Arg Phe Lys Gly Pro Cys Ala Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Tyr Gly Gly

20 25 30

Asn Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Asn Cys

35 40 45

<210> 347

<211> 47

<212> PRT

<213> Hydrangea (HYDRANGEA MACROPHYLLA)

<400> 347

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Ala Thr Asn Cys Gly Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 348

<211> 71

<212> PRT

<213> Vanilla (Vanilla planifolia)

<400> 348

Arg Arg Cys Arg Ala Gln Ser Phe Thr Phe Lys Gly Ile Cys Tyr Ser

1 5 10 15

Ser His Ser Cys Ala Leu Val Cys Lys Lys Glu Gly Tyr Glu Thr Gly

20 25 30

His Cys Ser Arg Gly Phe Cys Thr Leu Leu Lys Pro Ala Asn Ala Glu

35 40 45

Gln Arg Ala Gln Ser Leu Gln Val Phe Ala Ser Val Pro Thr Pro Ala

50 55 60

Leu Leu Cys Val Ser Lys Lys

65 70

<210> 349

<211> 58

<212> PRT

<213> Chickpea (Cicer arietinum)

<400> 349

Ser Glu Val Thr Ile Arg Val Ala Glu Gly Arg Glu Cys Arg Asp Leu

1 5 10 15

Ser His Lys Phe Lys Gly Phe Cys Val Arg Asp Arg Asn Cys Ala Asn

20 25 30

Val Cys Ser Thr Glu Gly Phe Thr Asn Gly His Cys Asp Gly Phe Arg

35 40 45

Arg Arg Cys Phe Cys Ser Arg Pro Cys Ala

50 55

<210> 350

<211> 49

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 350

Arg Val Cys Glu Thr Pro Ser His Lys Phe Gln Gly Ile Cys Ile Ser

1 5 10 15

Ser Arg Asn Cys Ala Ser Thr Cys Ala Ser Glu His Phe Pro Gly Gly

20 25 30

Tyr Cys Arg Gly Leu Arg Arg Arg Cys Met Cys Thr Arg Pro Cys Val

35 40 45

Asn

<210> 351

<211> 47

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 351

Arg Thr Cys Glu Thr Pro Ser Arg Lys Phe Arg Gly Ile Cys Val Ser

1 5 10 15

Arg Thr Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Leu Glu His Cys

35 40 45

<210> 352

<211> 47

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 352

Arg Ile Cys Glu Ser Pro Ser Tyr Ser Phe Lys Gly Ile Cys Val Ser

1 5 10 15

Arg Thr Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Leu Lys His Cys

35 40 45

<210> 353

<211> 47

<212> PRT

<213> Spinach (Spinacea oleracea)

<400> 353

Arg Thr Cys Glu Ser Arg Ser Tyr Arg Phe Lys Gly Ile Cys Val Ser

1 5 10 15

Arg Thr Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Val Arg Arg Arg Cys Phe Cys Leu Lys His Cys

35 40 45

<210> 354

<211> 47

<212> PRT

<213> Beet subspecies vulgaris (Beta vulgaris subsp. Vulgaris)

<400> 354

Arg Thr Cys Glu Thr Pro Ser His Lys Phe Lys Gly Ile Cys Val Ser

1 5 10 15

Arg Thr Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Pro Ala Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Leu Thr His Cys

35 40 45

<210> 355

<211> 47

<212> PRT

<213> Sanzhitoea triloba (Ipomoea triloba)

<400> 355

Arg Ile Cys Glu Ser Lys Ser His His Tyr Lys Gly Met Cys Leu Arg

1 5 10 15

Asp His Asn Cys Ala Met Val Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

His Cys Ile Gly Ala Arg Arg Arg Cys Phe Cys Thr Arg Gly Cys

35 40 45

<210> 356

<211> 47

<212> PRT

<213> White lupin (Lupinus albus)

<400> 356

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Ala Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Gly Ser Val Cys His Asn Glu Gly Phe Asn Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 357

<211> 52

<212> PRT

<213> White lupin (Lupinus albus)

<400> 357

Phe Leu Gly Ser His Arg Cys Gly Lys Val Leu Ser Ser Arg Phe Lys

1 5 10 15

Gly Lys Cys Leu Thr Asp His Asn Cys Asp Ser Val Cys Gln Leu Glu

20 25 30

Gly Tyr Val Gly Gly Asp Cys His Gly Val Arg Arg Arg Cys Phe Cys

35 40 45

Asn Lys Lys Cys

50

<210> 358

<211> 47

<212> PRT

<213> White lupin (Lupinus albus)

<400> 358

Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Leu Cys His Ser

1 5 10 15

Asp His Asn Cys Ala Ser Ile Cys Leu Val Glu Gly Phe Thr Ser Gly

20 25 30

Lys Cys His Gly Phe Arg Gln Arg Cys Phe Cys Thr Lys Val Cys

35 40 45

<210> 359

<211> 49

<212> PRT

<213> White lupin (Lupinus albus)

<400> 359

Arg Thr Arg Val Cys Glu Ser Gln Ser His Leu Phe Lys Gly Pro Cys

1 5 10 15

Ser Arg Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Ala Phe Ser

20 25 30

Gly Gly Arg Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu

35 40 45

Cys

<210> 360

<211> 59

<212> PRT

<213> White lupin (Lupinus albus)

<400> 360

Met Val Ile Glu Thr Glu Gly Val Leu Gly Arg Asn Arg Cys Asp Pro

1 5 10 15

Val Leu Ser Ser Arg Phe Lys Gly Lys Cys Phe Ser Asp His Asn Cys

20 25 30

Asn Ser Val Cys Gln Leu Glu Gly Tyr Thr Asp Gly Asn Cys His Gly

35 40 45

Leu Arg Gln Arg Cys Phe Cys Asn Arg Lys Cys

50 55

<210> 361

<211> 59

<212> PRT

<213> White lupin (Lupinus albus)

<400> 361

Ser Asp Asn Asn Val Gly Leu Lys Arg Ala Glu Gly Arg Val Cys Gln

1 5 10 15

Ser Lys Ser His Arg Phe Lys Gly Pro Cys Leu Ser Tyr Ser Asn Cys

20 25 30

Gly His Val Cys Arg Asn Glu Gly Phe Ser Gly Gly Arg Cys Arg Gly

35 40 45

Leu Arg Arg Arg Cys Phe Cys Thr Lys Asn Cys

50 55

<210> 362

<211> 59

<212> PRT

<213> White lupin (Lupinus albus)

<400> 362

Glu Met Gly Pro Thr Met Met Glu Glu Ala Arg Thr Cys Lys Ser Lys

1 5 10 15

Ser Gln Arg Tyr Lys Gly Ser Cys Tyr Ser Asp Asn Asn Cys Ala Ile

20 25 30

Ile Cys Glu Gly Glu Gly Phe Thr Gly Gly Lys Cys His Gly Phe Phe

35 40 45

Cys Lys Cys Trp Cys Lys Arg Pro Cys Leu Asp

50 55

<210> 363

<211> 69

<212> PRT

<213> White lupin (Lupinus albus)

<400> 363

Lys Glu Cys Ser Thr Lys Ser Asp Arg Phe Arg Gly Thr Cys Phe Arg

1 5 10 15

Ser Ser Asn Cys Ala Ala Val Cys Asn Ser Glu Asn Phe Pro Ser Gly

20 25 30

Thr Cys Arg Gly Phe Pro Gly Thr Cys Tyr Cys Thr Ser Asn Cys Ala

35 40 45

Ile Asn Ala Pro Ile Thr Asn Pro Pro Pro Glu Val Val Glu Pro Pro

50 55 60

Pro Glu Val Val Glu

65

<210> 364

<211> 48

<212> PRT

<213> White lupin (Lupinus albus)

<400> 364

Lys Thr Cys Glu Ser Arg Ser His Lys Leu Lys Gly Phe Cys Tyr Ser

1 5 10 15

Ser Lys Arg Cys Ala His Val Cys Thr Ser His His Asn Phe Ile Gly

20 25 30

Gly His Cys Arg Gly Phe Ile Arg Arg Cys His Cys Thr Lys Pro Cys

35 40 45

<210> 365

<211> 48

<212> PRT

<213> White lupin (Lupinus albus)

<400> 365

Asn Asn Cys Lys Tyr Glu Ser Asn Lys Phe His Gly Val Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Glu Ser Val Cys Gln Thr Glu Gly Phe Pro Phe Gly

20 25 30

Lys Cys Asp Gly Val Leu Pro Arg Thr Cys Val Cys Lys Lys Pro Cys

35 40 45

<210> 366

<211> 47

<212> PRT

<213> Light She Baimai root (Lotus japonica)

<400> 366

Arg His Cys Glu Ser Lys Ser His Arg Phe His Gly Leu Cys Leu Ser

1 5 10 15

His His Asn Cys Ala Ser Val Cys His Leu Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Lys Arg Cys Phe Cys Lys Lys Arg Cys

35 40 45

<210> 367

<211> 48

<212> PRT

<213> Light She Baimai root (Lotus japonica)

<400> 367

Arg Val Cys Gln Ser Gln Ser His Arg Phe Arg Gly Gly Cys Trp Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys Asn

35 40 45

<210> 368

<211> 47

<212> PRT

<213> Light She Baimai root (Lotus japonica)

<400> 368

Arg Val Cys Glu Thr Lys Ser His Thr Phe His His Ser Cys Val Ser

1 5 10 15

Gly His Asn Cys Ala Gln Val Cys Lys Thr Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Arg Cys

35 40 45

<210> 369

<211> 47

<212> PRT

<213> Light She Baimai root (Lotus japonica)

<400> 369

Arg Asp Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys His Gly Glu Lys Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 370

<211> 55

<212> PRT

<213> Lettuce (Lactuca sativa)

<400> 370

Met Gly Val Glu Thr Lys Ser Cys Arg Tyr Ser Ser Lys Thr Tyr Lys

1 5 10 15

Gly Leu Cys Phe Tyr Ser Arg Asn Cys Asp Ile Ile Cys Lys Ala Glu

20 25 30

Gly Ala Pro Gly Gly Gly His Cys Gln Ile Leu Thr Leu Arg Cys Phe

35 40 45

Cys Asn Val Cys Asp Pro Asp

50 55

<210> 371

<211> 47

<212> PRT

<213> Cassava (Manihot esculenta)

<400> 371

Arg Ile Cys Glu Ser Gln Ser His Arg Phe Lys Gly Val Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Ala Ile Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 372

<211> 47

<212> PRT

<213> Cassava (Manihot esculenta)

<400> 372

Arg Val Cys Glu Ser Lys Ser His His Phe Arg Gly Leu Cys Thr Ile

1 5 10 15

Ala His Asn Cys Ala Met Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 373

<211> 47

<212> PRT

<213> Cassava (Manihot esculenta)

<400> 373

Arg Val Cys Glu Ser Lys Ser His His Phe Arg Gly Val Cys Thr Ile

1 5 10 15

Ala His Asn Cys Ala Ile Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 374

<211> 47

<212> PRT

<213> Cassava (Manihot esculenta)

<400> 374

Arg Lys Cys Glu Ser Gln Ser His Lys Phe Lys Gly Ile Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Lys Gly Ala Arg Arg Arg Cys Phe Cys Thr Arg Gln Cys

35 40 45

<210> 375

<211> 47

<212> PRT

<213> Cassava (Manihot esculenta)

<400> 375

Arg Lys Cys Glu Ser Gln Ser His Arg Phe Lys Gly Met Cys Thr Arg

1 5 10 15

His Ser Asn Cys Ala Ala Ile Cys Gln Thr Glu Gly Phe His Ala Gly

20 25 30

His Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 376

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 376

Arg Val Cys Gln Ser Gln Ser His Gly Phe His Gly Thr Cys Leu Arg

1 5 10 15

His His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 377

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 377

Arg Thr Glu Ser Ser Lys Ala Val Glu Gly Lys Ile Cys Glu Phe Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Leu Leu Thr

35 40 45

Arg Thr Cys Met Cys Thr Lys Lys Cys

50 55

<210> 378

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 378

Arg Thr Cys Glu Ser Gln Ser Asn Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 379

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 379

Arg Thr Cys Glu Ser His Ser Arg Lys Tyr Lys Gly Thr Cys Leu Gly

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 380

<211> 63

<212> PRT

<213> Apple (Malus domestica)

<400> 380

Arg Val Cys Glu Val Pro Ser Lys Thr Phe Arg Gly Leu Cys Phe Ser

1 5 10 15

Asp Thr His Cys Ile Val Lys Cys His Ser Glu Gly Phe Gly Tyr Gly

20 25 30

Lys Cys Ser His Val Leu Arg Gln Cys Arg Cys Leu Lys Ala Cys Gly

35 40 45

Arg Leu Ala Glu Ser Glu Thr Thr His Gly Thr Glu Thr Ser Pro

50 55 60

<210> 381

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 381

Arg Thr Glu Pro Ser Lys Ala Val Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Ile Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly His Cys Ser Val Phe Thr

35 40 45

Arg Ala Cys Val Cys Thr Lys Lys Cys

50 55

<210> 382

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 382

Arg Val Glu Ser Ser Lys Ala Val Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Cys Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly His Cys Ser Val Phe Thr

35 40 45

Arg Ala Cys Val Cys Thr Lys Lys Cys

50 55

<210> 383

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 383

Arg Thr Glu Ser Asn Lys Ala Val Glu Gly Lys Ile Cys Glu Tyr Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly His Cys Ser Val Phe Thr

35 40 45

Arg Ala Cys Val Cys Thr Lys Lys Cys

50 55

<210> 384

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 384

Lys Thr Asn Glu Asn Ser Ser Lys Asn Glu Lys Ser Ser Lys Ile Cys

1 5 10 15

Glu Ser Pro Ser His Lys Tyr Arg Gly Ile Cys Phe Arg Ser Ser Asn

20 25 30

Cys Ala Thr Thr Cys Lys Lys Glu Gly Phe Met Gly Gly Lys Cys Ala

35 40 45

Gly Phe Arg Met Arg Cys Val Cys Thr Lys Lys Cys

50 55 60

<210> 385

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 385

Lys Thr Lys Glu Glu Ser Ser Lys Asn Glu Lys Ser Ser Lys Ile Cys

1 5 10 15

Glu Ser Pro Ser His Lys Tyr Arg Gly Ile Cys Phe Arg Ser Ser Asn

20 25 30

Cys Ala Thr Thr Cys Lys Lys Glu Gly Phe Met Gly Gly Lys Cys Ala

35 40 45

Gly Phe Arg Met Arg Cys Val Cys Thr Lys Lys Cys

50 55 60

<210> 386

<211> 59

<212> PRT

<213> Apple (Malus domestica)

<400> 386

Lys Thr Glu Ser Ser Lys Ala Val Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Ile Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ile Gly Asp Gly

35 40 45

Phe Thr Arg Lys Cys Val Cys Thr Lys Lys Cys

50 55

<210> 387

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 387

Arg Ser Lys Ser Ser Lys Glu Val Gln Glu Arg Thr Cys Glu Ala Ala

1 5 10 15

Ser Gly Lys Phe Lys Gly Leu Cys Phe Ser Ser Thr Asn Cys Lys Asn

20 25 30

Thr Cys Lys Arg Glu Gln Phe Thr Gly Gly Lys Cys Arg Gly Phe Arg

35 40 45

Arg Arg Cys Met Cys Asn Lys Lys Cys

50 55

<210> 388

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 388

Arg Ser Lys Ser Asp Lys Val Ala Lys Glu Arg Thr Cys Glu Ala Ala

1 5 10 15

Ser Gly Lys Phe Lys Gly Leu Cys Phe Ser Ser Thr Asn Cys Lys Asn

20 25 30

Thr Cys Lys Val Glu Lys Phe Thr Gly Gly Gln Cys Gln Gly Phe Arg

35 40 45

Arg Arg Cys Met Cys Asn Lys Lys Cys

50 55

<210> 389

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 389

Arg Ser Lys Ser Gly Lys Ala Val Lys Glu Arg Thr Cys Glu Ala Thr

1 5 10 15

Ser Gly Lys Phe Lys Gly Met Cys Phe Ser Ser Thr Asn Cys Lys Asn

20 25 30

Thr Cys Lys Thr Glu Lys Phe Ile Gly Gly Glu Cys His Gly Phe Arg

35 40 45

Arg Arg Cys Ile Cys Asn Lys Lys Cys

50 55

<210> 390

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 390

Arg Thr Glu Ser Ser Lys Ala Ile Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Gly Leu Thr

35 40 45

Arg Thr Cys Val Cys Thr Lys Lys Cys

50 55

<210> 391

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 391

Arg Thr Glu Ser Ser Lys Ala Val Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Gly Leu Thr

35 40 45

Arg Thr Cys Met Cys Thr Lys Lys Cys

50 55

<210> 392

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 392

Met Thr Glu Ser Asn Lys Ala Val Glu Gly Lys Ile Cys Glu Val Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Gly Leu Thr

35 40 45

Arg Thr Cys Met Cys Thr Lys Lys Cys

50 55

<210> 393

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 393

Val Glu Ala Arg Ser Lys Ser Ser Lys Val Val Glu Glu Arg Thr Cys

1 5 10 15

Glu Ala Ala Ser Gly Lys Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn

20 25 30

Cys Lys His Thr Cys Lys Arg Glu Gln Phe Thr Gly Gly Lys Cys Arg

35 40 45

Gly Phe Arg Arg Arg Cys Met Cys Asn Lys Lys Cys

50 55 60

<210> 394

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 394

Arg Thr Cys Asp Ser Gln Ser His Arg Phe Lys Gly Ser Cys Val Ser

1 5 10 15

Lys Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Arg Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 395

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 395

Lys Thr Lys Glu Lys Ser Ser Lys Asn Glu Lys Ser Ser Lys Thr Cys

1 5 10 15

Glu Ser Pro Ser His Lys Tyr His Gly Ile Cys Phe Arg Ser Ser Asn

20 25 30

Cys Ala Thr Thr Cys Lys Lys Glu Gly Phe Met Gly Gly Lys Cys Thr

35 40 45

Gly Phe Trp Met Arg Cys Val Cys Thr Lys Lys Cys

50 55 60

<210> 396

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 396

Lys Thr Lys Glu Lys Ser Ser Lys Asn Glu Lys Ser Ser Lys Ile Cys

1 5 10 15

Glu Ser Pro Ser His Lys Phe His Gly Ile Cys Phe Arg Ser Ser Asn

20 25 30

Cys Ala Thr Thr Cys Lys Lys Glu Gly Phe Met Gly Gly Lys Cys Thr

35 40 45

Gly Phe Arg Met Arg Cys Val Cys Thr Lys Lys Cys

50 55 60

<210> 397

<211> 61

<212> PRT

<213> Apple (Malus domestica)

<400> 397

Lys Ile Arg His Met Ser Lys Asp Glu Asn Ser Leu Thr Cys Glu Ser

1 5 10 15

Arg Ser His Lys Tyr His Gly Pro Cys Phe Arg Phe Ile Phe Ser Ser

20 25 30

Asn Cys Ala Val Thr Cys Lys Gly Glu Gly Phe Thr Gly Gly Lys Cys

35 40 45

Lys Gly Phe His Met Arg Cys Ile Cys Thr Lys Gln Cys

50 55 60

<210> 398

<211> 59

<212> PRT

<213> Apple (Malus domestica)

<400> 398

Lys Thr Lys Gly Lys Pro Ser Lys Asn Val Lys Leu Ser Lys Thr Cys

1 5 10 15

Glu Ser Pro Ser Gln Lys Tyr Pro Gly Ile Cys Arg Ser Ser Tyr Cys

20 25 30

Ala Ala Ile Cys Lys Gln Gln Gly Leu Met Gly Gly Lys Cys Thr Gly

35 40 45

Phe Arg Arg Lys Cys Val Cys Thr Lys Lys Cys

50 55

<210> 399

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 399

Arg Thr Glu Ser Ser Lys Ala Ile Glu Gly Lys Ile Cys Glu Phe Pro

1 5 10 15

Ser Thr Leu Phe Lys Gly Leu Cys Phe Ser Ser Asn Asn Cys Lys His

20 25 30

Thr Cys Arg Lys Glu Gln Phe Thr Arg Gly Lys Cys Ser Leu Leu Thr

35 40 45

Arg Thr Cys Met Cys Thr Lys Lys Cys

50 55

<210> 400

<211> 60

<212> PRT

<213> Apple (Malus domestica)

<400> 400

Arg Ser Gly Met Thr Ser Lys Gly Ala Ala Glu Lys Lys Lys Cys Asp

1 5 10 15

Ser His Pro Ser Ser Lys Phe Lys Gly Val Cys Phe Phe Thr Asn Asn

20 25 30

Cys Val Asp Thr Cys Lys Leu Glu Gly Ser Ser Gly Gly Gln Cys Arg

35 40 45

Gly Phe Arg Arg Arg Cys Ile Cys Thr Lys Gln Cys

50 55 60

<210> 401

<211> 57

<212> PRT

<213> Apple (Malus domestica)

<400> 401

Lys Ser Lys Ser Ser Lys Glu Val Glu Lys Arg Thr Cys Glu Ala Ala

1 5 10 15

Ser Gly Lys Phe Lys Gly Met Cys Phe Ser Ser Asn Asn Cys Ala Asn

20 25 30

Thr Cys Ala Arg Glu Lys Phe Asp Gly Gly Lys Cys Lys Gly Phe Arg

35 40 45

Arg Arg Cys Met Cys Thr Lys Lys Cys

50 55

<210> 402

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 402

Arg Thr Cys Glu Ser Gln Ser Asn Arg Phe Lys Gly Ser Cys Val Ser

1 5 10 15

Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 403

<211> 47

<212> PRT

<213> Apple (Malus domestica)

<400> 403

Arg Thr Cys Glu Ser Gln Ser Arg Lys Phe Lys Gly Thr Cys Leu Ser

1 5 10 15

Lys Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 404

<211> 57

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 404

Gln Pro Thr His Val Glu Glu Pro Glu Ala Arg Thr Cys Asp Ser Gln

1 5 10 15

Ser His Ser Phe Lys Gly Val Cys Trp Ile Lys His Asn Cys Ala Asn

20 25 30

Val Cys Lys Thr Glu Gly Phe Thr Gly Gly His Cys His Gly Phe Arg

35 40 45

Arg Arg Cys Phe Cys Ser Lys Pro Cys

50 55

<210> 405

<211> 76

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 405

Glu Glu Phe Asn Phe Ala Pro Phe Thr Pro Leu His Phe Arg Val Glu

1 5 10 15

Ser Cys Phe Thr Arg Ser Arg Arg Tyr Asn Gly Ile Cys Leu Ser Asp

20 25 30

Glu Ser Cys Phe Lys Ala Cys Ser Leu Glu Gly Tyr Ala Cys Gly Arg

35 40 45

Cys Glu Lys Arg Arg Pro Tyr Leu Gly Asp His Cys Cys Cys Lys Lys

50 55 60

Ser Cys Gln Leu Lys Leu Val Leu Ala Val Leu Cys

65 70 75

<210> 406

<211> 50

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 406

Arg Lys His Cys Arg Glu Lys Ser Arg Leu Phe Glu Glu Leu Cys Phe

1 5 10 15

Asn Ser Glu Asp Cys Ala Asn Thr Cys Arg Tyr Glu Gly Phe His Leu

20 25 30

Gly Gly Lys Cys Trp Gly Leu Phe Arg Thr Cys Tyr Cys Lys Lys Lys

35 40 45

Cys Arg

50

<210> 407

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 407

Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Met Cys Met Ser

1 5 10 15

Asp His Asn Cys Ala Ser Val Cys His Val Glu Gly Phe Pro Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Lys Lys Arg Cys

35 40 45

<210> 408

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 408

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Lys Gly Pro Cys Ala Ser

1 5 10 15

Asp His Asn Cys Ala Ser Val Cys Gln Thr Glu Arg Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys

35 40 45

<210> 409

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 409

Arg Thr Cys Glu Thr Pro Ser Asn Ser Phe Lys Gly Ala Cys Phe Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

His Cys Lys Gly Phe Arg Gln Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 410

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 410

Arg Thr Cys Glu Thr Pro Ser Asn Asn Phe Lys Gly Leu Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

His Cys Glu Gly Phe Arg Gln Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 411

<211> 50

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 411

Arg Lys Cys Leu Ser Gln Ser His Ser Phe Lys Gly Leu Cys Leu Ser

1 5 10 15

Asp Gln Asn Cys Ala Thr Val Cys Leu Thr Glu Gly Phe Thr Asp Gly

20 25 30

Arg Cys Arg Gly Phe Arg Gln Arg Cys Phe Cys Ser Lys Pro Cys Leu

35 40 45

Lys Val

50

<210> 412

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 412

Arg Arg Cys Glu Ser Lys Ser His Lys Phe Lys Gly Pro Cys Ser Arg

1 5 10 15

Asp Ser Asn Cys Ala Ser Val Cys Arg Gly Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 413

<211> 47

<212> PRT

<213> Tribulus alfalfa (Medicago truncatula)

<400> 413

Arg Arg Cys Glu Ser Gln Ser His Lys Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Ser Asn Cys Gly Ser Val Cys Arg Gly Glu Gly Phe Ile Gly Gly

20 25 30

Asp Cys Arg Gly Val Arg His Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 414

<211> 48

<212> PRT

<213> Melon (Cucumis melo)

<400> 414

Arg Val Cys Glu Ser Pro Ser His Asn Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Asp Thr Asn Cys Gly Asn Ile Cys Lys Thr Glu Gly Phe Ser Gly Gly

20 25 30

Leu Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys Val

35 40 45

<210> 415

<211> 47

<212> PRT

<213> Melon (Cucumis melo)

<400> 415

Arg Met Cys Gln Ser Gln Ser His His Phe His Gly Thr Cys Ile Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

Glu Cys Val Gly Phe Arg Arg Arg Cys Phe Cys Thr His Arg Cys

35 40 45

<210> 416

<211> 58

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 416

Val Lys Glu Val Gly Thr Val Glu Asp Glu Lys Lys Val Cys Lys Tyr

1 5 10 15

Lys Ser Lys Lys Phe Trp Gly Val Cys Leu Ser Asp Ile Arg Cys Thr

20 25 30

Asp Val Cys Lys Arg Glu Gly Phe Gln Gly Gly Asp Cys Gln Gly Ile

35 40 45

Arg Arg Arg Cys Leu Cys Tyr Asn Arg Cys

50 55

<210> 417

<211> 48

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 417

Thr Arg Thr Cys Glu Ala Lys Ser His Arg Phe Lys Gly Ile Cys Ala

1 5 10 15

Arg Lys Ala Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly

20 25 30

Gly His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 418

<211> 47

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 418

Arg Val Cys Glu Ser Lys Ser Ala Ser Tyr Lys Gly Val Cys Val Leu

1 5 10 15

Asp Val Thr Cys Lys Leu Ala Cys Leu Asn Glu Gly Phe Ala Asp Gly

20 25 30

Asp Cys Glu Gly Val Arg Arg Arg Cys Met Cys Arg Arg Pro Cys

35 40 45

<210> 419

<211> 39

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 419

Phe Lys Gly Ile Cys Val Leu Asp Glu Thr Cys Arg Leu Ala Cys Leu

1 5 10 15

Asn Glu Gly Phe Ser Asp Gly Asp Cys Glu Gly Leu Arg Arg Arg Cys

20 25 30

Leu Cys Arg Lys Pro Cys Lys

35

<210> 420

<211> 72

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 420

Ala Leu Cys Glu Thr Lys Ser Ser Ile Phe Thr Gly Ala Cys Phe Ser

1 5 10 15

Asp Arg Asn Cys Glu Thr Ile Cys Glu Lys Glu Gly Phe Leu Asn Gly

20 25 30

Arg Cys Lys Leu Trp Lys Cys Ile Cys Ser Lys Asp Cys Gly Ile Gly

35 40 45

Gly Gly Gly Gly Val Val Val Pro Pro Gly Gly Gly Gly Asp Val Pro

50 55 60

Pro Gly Gly Gly Asp Asp Val Pro

65 70

<210> 421

<211> 48

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 421

Arg Met Cys Glu Thr Gln Ser His Gly Phe Lys Gly Gln Cys Trp Ser

1 5 10 15

Glu His Asn Cys Arg Leu Val Cys Ala His Glu Gly Phe Arg Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Lys Arg Cys Phe Cys Thr Arg Pro Cys His

35 40 45

<210> 422

<211> 47

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 422

Arg Thr Cys Leu Ser Gln Ser Gln Arg Phe Arg Gly Pro Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Arg Leu Val Cys His Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Gln Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 423

<211> 64

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 423

Gln Ser Asn Val Asp Gly Glu Ile Ala Asp Tyr Leu Lys Ile Glu Glu

1 5 10 15

Lys Lys Arg Cys Asn Tyr Glu Ser Lys Lys Phe Trp Gly Ile Cys Leu

20 25 30

Ser Asp Ser Lys Cys Gly Glu Val Cys Thr Lys Glu Gly Phe Glu Gly

35 40 45

Gly Asp Cys Lys Gly Leu Arg Arg Arg Cys Tyr Cys Phe Asn Asn Cys

50 55 60

<210> 424

<211> 71

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 424

Ser Leu Cys Glu Thr Pro Ser Arg Tyr Phe Asn Gly Ile Cys Phe Ser

1 5 10 15

Arg Arg Asn Cys Gly Ala Ile Cys Glu Lys Glu Gly Phe Ser Ser Gly

20 25 30

Arg Cys Lys Ala Phe Lys Cys Val Cys Gly Lys Asp Cys Asn Ala Ala

35 40 45

Gly Gly Gly Gly Gly Gly Gly Gly Gly Asp Pro Gly Leu Gly Pro Pro

50 55 60

Val Glu Gly Pro Pro Asp Gln

65 70

<210> 425

<211> 47

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 425

Arg Thr Cys Glu Ser Gln Ser His Gly Phe Lys Gly Arg Cys Leu Ser

1 5 10 15

Asp His Asn Cys Gly Met Val Cys Lys Asn Glu Gly Phe Ser Asn Gly

20 25 30

Trp Cys Arg Gly Phe Arg Gln Arg Cys Phe Cys Thr Arg Ala Cys

35 40 45

<210> 426

<211> 47

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 426

Arg Thr Cys Thr Ser Lys Ser Ser Asn Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Ser His Asn Cys Lys Ala Val Cys Asn Gly Glu Asn Phe Pro Asp Gly

20 25 30

Lys Cys His Gly Phe Lys Arg Arg Cys Phe Cys Gln Lys Pro Cys

35 40 45

<210> 427

<211> 47

<212> PRT

<213> Day lily flower (Mimulus guttatus)

<400> 427

Arg Thr Cys Glu Ser Gln Ser Phe Asn Phe Arg Gly Pro Cys Val Ile

1 5 10 15

Asp His Asn Cys Lys Ala Val Cys Glu Ser Glu Gly Phe Thr Asp Gly

20 25 30

Asp Cys Gln Gly Phe Arg Arg Arg Cys Phe Cys Arg Lys Pro Cys

35 40 45

<210> 428

<211> 62

<212> PRT

<213> Aeolian bells wood (Handroanthus impetiginosus)

<400> 428

Ala Leu Cys Glu Lys Pro Ser Arg Leu Tyr Asn Gly Ser Cys Thr Glu

1 5 10 15

Glu Arg Leu Cys Ile Glu Ile Cys Gln Lys Glu Ala Tyr Pro Ser Gly

20 25 30

Arg Cys Asp Leu Leu Lys Cys Met Cys Ile Asn Lys Cys Pro Pro Val

35 40 45

Gly Ala Gly Gln Gly Ala Gly Ser Asp Gly Gln Arg Gly Ile

50 55 60

<210> 429

<211> 47

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 429

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Ser Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Leu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 430

<211> 47

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 430

Arg His Cys Glu Ser Gln Ser Gln Arg Phe Arg Gly Leu Cys Val Arg

1 5 10 15

Glu Lys Asn Cys Ala Ala Val Cys Glu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 431

<211> 47

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 431

Arg Val Cys Met Ser Pro Ser His Gly Tyr His Gly Pro Cys Trp His

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Val Gly Ile Gln Arg Lys Cys Tyr Cys Thr Lys Leu Cys

35 40 45

<210> 432

<211> 47

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 432

Arg Thr Cys Glu Ser Gln Ser His Arg Phe His Gly Thr Cys Val Arg

1 5 10 15

Gly Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 433

<211> 58

<212> PRT

<213> Tobacco (Nicotiana tabacum)

<400> 433

Thr Glu Met Gly Pro Met Thr Ile Ala Glu Ala Arg Arg Cys Glu Ser

1 5 10 15

Lys Ser Gln Arg Phe Arg Gly Pro Cys Val Arg Glu Lys Asn Cys Ala

20 25 30

Ala Val Cys Glu Thr Glu Gly Phe Ser Gly Gly Asp Cys Arg Gly Leu

35 40 45

Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

50 55

<210> 434

<211> 48

<212> PRT

<213> Lotus (Nelumbo nucifera)

<400> 434

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Ile Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys Ala

35 40 45

<210> 435

<211> 13

<212> PRT

<213> Lotus (Nelumbo nucifera)

<400> 435

Cys Arg Asp Gly Ala Lys Ser Ser Arg Gly Lys Asp Leu

1 5 10

<210> 436

<211> 49

<212> PRT

<213> Lotus (Nelumbo nucifera)

<400> 436

Lys Met Cys Lys Thr Pro Ser Arg Gln Phe Arg Gly Leu Cys Leu Ile

1 5 10 15

Gly Arg Asn Cys Asp Asn Val Cys Arg Gly Glu Gly Phe Pro Asp Gly

20 25 30

Asp Cys Asp Gly Phe Arg Arg Arg Cys Ile Cys Ser Arg Pro Cys Arg

35 40 45

Pro

<210> 437

<211> 100

<212> PRT

<213> Lotus (Nelumbo nucifera)

<400> 437

Arg Met Cys Asp Thr Pro Ser Gln Lys Phe Val Gly Leu Cys Leu Ile

1 5 10 15

Asp Arg Asn Cys Glu Asn Val Cys Lys Thr Glu Gly Phe Pro Ala Gly

20 25 30

His Cys Glu Gly Leu Arg Arg Arg Cys Phe Cys Ser Glu Pro Cys Pro

35 40 45

Pro Asn Asn Gly Met Glu Arg Lys Phe Leu Cys Gly Val Leu Leu Phe

50 55 60

Leu Thr Leu Leu Tyr Ala Ser Leu Gly Glu Glu Met Val Val Asn Glu

65 70 75 80

Gly Pro Ala Asp Gly Gly Arg Val Cys Ser Thr Pro Ser Leu Asn Tyr

85 90 95

Arg Gly Pro Cys

100

<210> 438

<211> 49

<212> PRT

<213> Lotus (Nelumbo nucifera)

<400> 438

Arg Met Cys Asp Thr Pro Ser Arg Leu Phe Arg Gly Leu Cys Leu Ile

1 5 10 15

Asn Arg Asn Cys Asp Asn Thr Cys Arg Val Glu Gly Phe Pro Asp Gly

20 25 30

His Cys Lys Gly Leu Arg Arg Arg Cys Phe Cys Ser Arg Pro Cys Arg

35 40 45

Pro

<210> 439

<211> 49

<212> PRT

<213> Olive (Olea europaea)

<400> 439

Arg Ile Cys Glu Ser Leu Ser His Arg Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Asn Ala Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Pro Cys Ser

35 40 45

Asp

<210> 440

<211> 47

<212> PRT

<213> Olive (Olea europaea)

<400> 440

Arg Ile Cys Glu Ser Leu Ser His Arg Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Arg Gly Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Leu Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 441

<211> 70

<212> PRT

<213> Rice (Oryza sativa)

<400> 441

Ile Ile Cys Lys Ala Arg Ser Lys Met Tyr Arg Gly Lys Cys Arg Gly

1 5 10 15

Asn Arg Asn Cys Ala Met Ile Cys Val His Glu Glu Tyr Thr Gly Gly

20 25 30

Tyr Cys Ser Lys Gly Val Phe Ser Lys Cys Met Cys Thr Lys Arg Cys

35 40 45

Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Asp

50 55 60

Glu Pro Pro Leu Arg Glu

65 70

<210> 442

<211> 70

<212> PRT

<213> Rice (Oryza sativa)

<400> 442

Ile Cys His Asp His Ser Gln Thr Phe Lys Gly Met Cys Phe His Thr

1 5 10 15

Ser Asn Cys Ile Ala Cys Cys Thr Asn Glu Gly Tyr Thr Gly Gly Tyr

20 25 30

Cys Lys Pro Phe Thr Tyr Arg Cys Met Cys Thr Lys Asp Cys Gly Gly

35 40 45

Asp Ser Pro Pro Asp Asp Pro Pro Pro Ala Met Pro Thr Ser Pro Ala

50 55 60

Ala Thr Thr Thr Val Ala

65 70

<210> 443

<211> 59

<212> PRT

<213> Rice (Oryza sativa)

<400> 443

Arg Leu Cys Glu Lys Pro Ser Ser His Phe Lys Gly Leu Cys Leu Arg

1 5 10 15

Ser Gln Asn Cys Asp Asn Glu Cys Met Leu Glu Gly Tyr Met Asp Gly

20 25 30

Lys Cys Lys Tyr Leu Thr Arg Arg Cys Ile Cys Ser Met Glu Cys Ala

35 40 45

Thr Met Ser Asn Val Gly Leu Thr Pro Thr Lys

50 55

<210> 444

<211> 49

<212> PRT

<213> Rice (Oryza sativa)

<400> 444

Arg His Cys Leu Ser Gln Ser His Arg Phe Lys Gly Met Cys Val Ser

1 5 10 15

Ser Asn Asn Cys Ala Asn Val Cys Arg Thr Glu Ser Phe Pro Asp Gly

20 25 30

Glu Cys Lys Ser His Gly Leu Glu Arg Lys Cys Phe Cys Lys Lys Val

35 40 45

Cys

<210> 445

<211> 48

<212> PRT

<213> Rice (Oryza sativa)

<400> 445

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Ala Arg

1 5 10 15

Lys Ala Asn Cys Ala Ser Val Cys Asn Thr Glu Gly Phe Pro Asp Gly

20 25 30

Tyr Cys His Gly Val Arg Arg Arg Cys Met Cys Thr Lys Pro Cys Pro

35 40 45

<210> 446

<211> 49

<212> PRT

<213> Rice (Oryza sativa)

<400> 446

Arg His Cys Val Ser Gln Ser His Arg Phe Val Gly Ala Cys Met Arg

1 5 10 15

Lys Ser Asn Cys Glu His Val Cys Met Thr Glu Gly Phe Pro Trp Gly

20 25 30

Glu Cys Arg Phe His Gly Ile Glu Arg Lys Cys Phe Cys Lys Lys Arg

35 40 45

Cys

<210> 447

<211> 47

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 447

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 448

<211> 49

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 448

Arg Tyr Arg Val Cys Leu Ser Pro Ser His Arg Tyr Lys Gly Met Cys

1 5 10 15

Leu Arg Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser

20 25 30

Gly Gly Asp Cys Ile Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu

35 40 45

Cys

<210> 449

<211> 49

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 449

Glu Ala Arg Ile Cys Glu Ser Gln Ser His Arg Phe His Gly Thr Cys

1 5 10 15

Val Arg Glu Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Ile

20 25 30

Gly Gly Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn

35 40 45

Cys

<210> 450

<211> 47

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 450

Arg Thr Cys Glu Thr Pro Ser Gln Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Ala Asn Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Asp Cys Gln Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 451

<211> 47

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 451

Arg Thr Cys Glu Thr Leu Ser His Arg Phe Arg Gly Pro Cys Val Arg

1 5 10 15

Glu Lys Asn Cys Ala Ala Val Cys Glu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 452

<211> 47

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 452

Arg Val Cys Met Ser Pro Ser His Gly Tyr Arg Gly Pro Cys Ile Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asp Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Val Gly Ile Gln Arg Lys Cys Tyr Cys Thr Lys Leu Cys

35 40 45

<210> 453

<211> 50

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 453

Arg Thr Cys Glu His Arg Ser Gln His Tyr Tyr Gly Ala Cys Phe Ala

1 5 10 15

Asn Asn Ser Asn Met Asn Asn Asn Cys Asp Ser Met Cys Lys Cys Asp

20 25 30

Gly Phe Asp Gly Gly Phe Cys Arg Leu Thr Asn Cys Phe Cys His Val

35 40 45

Lys Cys

50

<210> 454

<211> 47

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 454

Lys Leu Cys Gln Tyr Lys Ser Lys Thr Phe His Gly Val Cys Val Thr

1 5 10 15

Gly Asn Thr Cys Asn Gln Lys Cys Gln Ser Glu Ala Phe Asp Gly Gly

20 25 30

Arg Cys Gln Gly Val Arg His Arg Cys Tyr Cys Tyr Arg Thr Cys

35 40 45

<210> 455

<211> 51

<212> PRT

<213> Horn flower (Petunia axillaris)

<400> 455

Arg Val Cys Arg Thr Asn Ser Lys Arg Tyr Ser Gly Pro Cys Phe Asn

1 5 10 15

Asn Asn Asn Asn Arg Asn Gln Asn Cys Asp Phe Ile Cys Arg Arg Cys

20 25 30

Glu Gly Phe Glu Gly Gly His Cys Ser Ser Arg Thr Cys Val Cys Thr

35 40 45

Lys Ser Cys

50

<210> 456

<211> 47

<212> PRT

<213> Granati (Punica granatum)

<400> 456

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Arg Gly Thr Cys Val Ser

1 5 10 15

Asn Thr Asn Cys Gly Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Gln Cys

35 40 45

<210> 457

<211> 47

<212> PRT

<213> Granati (Punica granatum)

<400> 457

Arg Ile Cys Glu Ser Lys Ser His Arg Phe Arg Gly Val Cys Met Ser

1 5 10 15

Asn Ser Asn Cys Ala Ser Val Cys His Asn Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Val Arg Lys Arg Cys Phe Cys Thr Lys Val Cys

35 40 45

<210> 458

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 458

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Lys Asn Cys Ala Ser Val Cys Glu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 459

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 459

Arg Asn Cys Glu Ser Leu Ser His Arg Phe Lys Gly Pro Cys Thr Arg

1 5 10 15

Asp Ser Asn Cys Ala Ser Val Cys Glu Thr Glu Arg Phe Ser Gly Gly

20 25 30

Asn Cys His Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 460

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 460

Arg Thr Cys Glu Ser Gln Ser Arg Arg Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Lys Ser Asn Cys Gly Ser Val Cys His Thr Glu Gly Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 461

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 461

Arg Thr Cys Glu Ser Gln Ser His Lys Tyr Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Gly Ala Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

His Cys Arg Gly Val Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 462

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 462

Arg Thr Cys Glu Ser Gln Ser His Ser Phe Lys Gly Pro Cys Val Gly

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Gln Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 463

<211> 47

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 463

Arg Thr Cys Glu Ser Gln Ser Asn Arg Phe Lys Gly Thr Cys Val Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 464

<211> 49

<212> PRT

<213> Potato (Solanum tuberosum)

<400> 464

Arg Val Cys Ile Ser Gln Ser His Arg Tyr Lys Gly Pro Cys Leu Arg

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Tyr Phe Ser Gly Gly

20 25 30

Asp Cys Ile Gly Phe Gly Phe His Arg Lys Cys Tyr Cys Thr Lys Phe

35 40 45

Cys

<210> 465

<211> 46

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 465

Lys Thr Cys Phe Thr Lys Lys Glu Gly Trp Gly Arg Cys Ile Ala Asp

1 5 10 15

Phe Thr Cys Ala Arg Ser Cys Arg Asn Val Gly Tyr Ile Gly Gly Lys

20 25 30

Cys Gln Gly Leu Thr Arg Arg Cys Phe Cys Val Leu Asn Cys

35 40 45

<210> 466

<211> 50

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 466

Ala Glu Gly Lys Ile Cys Arg Thr Lys Ser Arg Lys Tyr Thr Gly Ala

1 5 10 15

Cys Tyr Ser Asn Asp Ile Cys Ala Asn Tyr Cys Lys Gly Asp Gly Phe

20 25 30

Asp Asp Gly Asp Cys Gly Gly Leu Ser His Ile Cys Phe Cys Thr Lys

35 40 45

Asn Cys

50

<210> 467

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 467

Arg Leu Cys Glu Ser Pro Ser His Ser Phe Lys Gly Leu Cys Phe Asn

1 5 10 15

Asp Arg Asn Cys Ala Asn Val Cys Leu Thr Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Glu Gly Ala Arg His Arg Cys Phe Cys Thr Ile Ile Cys

35 40 45

<210> 468

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 468

Arg Leu Cys Trp Ser Glu Ser Gln Ala Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Asn Asp Asn Cys Ala Thr Val Cys Leu Thr Glu Gly Phe Thr Gly Gly

20 25 30

Lys Cys Glu Gly Leu Arg Arg Arg Cys Phe Cys Ser Lys Asn Cys

35 40 45

<210> 469

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 469

Arg Gly Cys Glu Ser Arg Ser Arg His Leu Arg Gly Lys Cys Glu Ser

1 5 10 15

Asp Glu Asn Cys Ala Ser Ala Cys Arg Thr Glu Arg Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Cys Arg Cys Phe Cys Asp Lys Arg Ser

35 40 45

<210> 470

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 470

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Arg Thr Glu Arg Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 471

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 471

Arg Val Cys Glu Ser Gln Ser His Gly Phe Lys Gly Ala Cys Thr Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Ile Cys

35 40 45

<210> 472

<211> 47

<212> PRT

<213> Beans (Phaseolus vulgaris)

<400> 472

Arg His Cys Glu Ser Lys Ser His Arg Phe Lys Gly Met Cys Leu Ser

1 5 10 15

Asp Arg Asn Cys Ala Ser Val Cys His Leu Glu Gly Phe Thr Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 473

<211> 47

<212> PRT

<213> Populus delphinii (Populus trichocarpus)

<400> 473

Arg Val Cys Leu Ser Gln Ser His His Tyr Lys Gly Pro Cys Leu Arg

1 5 10 15

Gly His Asn Cys Ala Met Val Cys Lys Thr Glu Gly Phe Ala Gly Gly

20 25 30

Glu Cys Lys Gly Phe Ile Ser Arg Cys Phe Cys Ala Lys Leu Cys

35 40 45

<210> 474

<211> 47

<212> PRT

<213> Populus delphinii (Populus trichocarpus)

<400> 474

Arg Val Cys Leu Ser Gln Ser His Ser Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Gly His Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Pro Gly Gly

20 25 30

Glu Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Ala Lys Pro Cys

35 40 45

<210> 475

<211> 47

<212> PRT

<213> Populus delphinii (Populus trichocarpus)

<400> 475

Arg Val Cys Leu Ser Gln Ser His Ser Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Gly His Asn Cys Ala Ser Val Cys Lys Thr Glu Gly Phe Pro Gly Gly

20 25 30

Glu Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Ala Lys Pro Cys

35 40 45

<210> 476

<211> 47

<212> PRT

<213> Populus delphinii (Populus trichocarpus)

<400> 476

Arg Val Cys Gln Ser Gln Ser His Tyr Phe Lys Gly Pro Cys Ala Arg

1 5 10 15

Asp His Asn Cys Ala Trp Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 477

<211> 47

<212> PRT

<213> Populus delphinii (Populus trichocarpus)

<400> 477

Arg Val Cys Gln Ser Gln Ser His Tyr Phe Lys Gly Pro Cys Ala Arg

1 5 10 15

Asp His Asn Cys Ala Trp Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Lys Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 478

<211> 47

<212> PRT

<213> Peach (Prunus persica)

<400> 478

Arg Val Cys Gln Ser Gln Ser His Gly Phe Arg Gly Pro Cys Ile Arg

1 5 10 15

His His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Leu Cys

35 40 45

<210> 479

<211> 47

<212> PRT

<213> Peach (Prunus persica)

<400> 479

Arg Thr Cys Glu Ser Gln Ser Asn Arg Phe Lys Gly Thr Cys Val Ser

1 5 10 15

Thr Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 480

<211> 57

<212> PRT

<213> Peach (Prunus persica)

<400> 480

Arg Asp Ala Lys Lys Val Glu Pro Lys Lys Lys Thr Cys Asp Ser Gln

1 5 10 15

Ser Gln Lys Phe Lys Gly Met Cys Phe Leu Thr Ser Ser Cys Val Gln

20 25 30

Ser Cys Lys Ser Glu Gly Phe Thr Gly Gly Gln Cys Arg Gly Phe Arg

35 40 45

Arg Arg Cys Phe Cys Ser Lys Asn Cys

50 55

<210> 481

<211> 57

<212> PRT

<213> Peach (Prunus persica)

<400> 481

Arg Ser Ala Lys Lys Val Glu Pro Lys Lys Arg Thr Cys Glu Ser Gln

1 5 10 15

Ser Gln Lys Phe Lys Gly Ile Cys Phe Leu Ser Ser Asn Cys Ala Thr

20 25 30

Ser Cys Thr Thr Glu Gly Phe Asn Gly Gly Gln Cys Arg Gly Phe Arg

35 40 45

Arg Arg Cys Phe Cys Ser Lys Ala Cys

50 55

<210> 482

<211> 57

<212> PRT

<213> Peach (Prunus persica)

<400> 482

Lys Ile Cys Glu Val Thr Ser Lys Thr Phe Ile Gly Leu Cys Ile Lys

1 5 10 15

Asp Arg Asn Cys Met Val Arg Cys His Ser Glu Gly Tyr Gly Tyr Gly

20 25 30

Lys Cys Ser His Ile Leu Arg Lys Cys Arg Cys Leu Lys Pro Cys Val

35 40 45

Ala Glu Glu Thr Lys Glu Asn Leu Pro

50 55

<210> 483

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 483

Lys Val Ala Thr Lys Glu Glu Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Phe Cys Leu Arg Ser Ser Arg Cys Ala Glu Ala Cys

20 25 30

Gln Lys Glu Gly Phe Met Gly Gly Lys Cys Pro Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Lys Cys

50 55

<210> 484

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 484

Lys Val Ala Thr Lys Glu Glu Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Phe Cys Leu Arg Ser Ser Arg Cys Ala Glu Ala Cys

20 25 30

Gln Lys Glu Gly Phe Met Gly Gly Lys Cys Pro Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Lys Cys

50 55

<210> 485

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 485

Lys Val Glu Thr Lys Glu Thr Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Pro Cys Ile Arg Ser Ser Asn Cys Ala Asn Ile Cys

20 25 30

Glu Glu Glu Gly Phe Lys Gly Gly Lys Cys Val Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Asn Cys

50 55

<210> 486

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 486

Lys Val Glu Thr Lys Glu Thr Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Pro Cys Ile Arg Ser Ser Asn Cys Ala Asn Ile Cys

20 25 30

Glu Glu Glu Gly Phe Lys Gly Gly Lys Cys Val Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Asn Cys

50 55

<210> 487

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 487

Lys Val Glu Thr Lys Glu Thr Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Pro Cys Ile Arg Ser Ser Asn Cys Ala Asn Ile Cys

20 25 30

Glu Glu Glu Gly Phe Lys Gly Gly Lys Cys Val Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Asn Cys

50 55

<210> 488

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 488

Lys Val Glu Thr Lys Glu Thr Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Pro Cys Ile Arg Ser Ser Asn Cys Ala Asn Ile Cys

20 25 30

Glu Glu Glu Gly Phe Lys Gly Gly Lys Cys Val Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Asn Cys

50 55

<210> 489

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 489

Lys Val Glu Thr Lys Glu Thr Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Pro Cys Phe Arg Ser Ser Asn Cys Ala Asn Ile Cys

20 25 30

Glu Lys Glu Gly Phe Lys Gly Gly Lys Cys Val Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Asn Cys

50 55

<210> 490

<211> 55

<212> PRT

<213> Peach (Prunus persica)

<400> 490

Lys Ala Ala Thr Lys Glu Asn Ser Arg Thr Cys Glu Ser Leu Ser Thr

1 5 10 15

Lys Phe Lys Gly Leu Cys Phe Arg Ser Ser Asn Cys Ala Asp Ile Cys

20 25 30

Lys Lys Glu Gly Phe Leu Glu Gly Lys Cys Ala Gly Phe Arg Leu Arg

35 40 45

Cys Thr Cys Thr Lys Lys Cys

50 55

<210> 491

<211> 47

<212> PRT

<213> Pea (Pisum sativum)

<400> 491

Arg Asp Cys Glu Ser Lys Ser His Lys Phe Lys Gly Thr Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Arg Phe Thr Gly Gly

20 25 30

His Cys Arg Gly Phe Arg His Arg Cys Tyr Cys Thr Thr His Cys

35 40 45

<210> 492

<211> 47

<212> PRT

<213> Pea (Pisum sativum)

<400> 492

Arg Asp Cys Glu Ser Pro Ser Gln Lys Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Pro Gly Gly

20 25 30

Lys Cys Glu Gly Leu Arg His Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 493

<211> 47

<212> PRT

<213> Pea (Pisum sativum)

<400> 493

Arg Asp Cys Glu Ser Pro Ser Gln Lys Phe Lys Gly Val Cys Leu Ser

1 5 10 15

Asp Arg Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys Lys Gly Leu Arg Gln Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 494

<211> 47

<212> PRT

<213> Pea (Pisum sativum)

<400> 494

Arg Asp Cys Glu Ser Pro Ser Gln Ile Phe Lys Gly Leu Cys Phe Thr

1 5 10 15

Asp Thr Asn Cys Ala Ser Val Cys His Thr Glu Gly Phe Pro Gly Gly

20 25 30

Lys Cys Lys Gly Leu Pro Lys Arg Cys Ile Cys Thr Lys Pro Cys

35 40 45

<210> 495

<211> 46

<212> PRT

<213> Pea (Pisum sativum)

<400> 495

Ala Gly Thr Cys Pro Ser Pro Ser Thr Lys Phe Lys Gly Val Cys Val

1 5 10 15

Ser Asp Phe Asp Cys Met Ile Thr Cys Gln Met Glu Gly Leu Ser Glu

20 25 30

Gly Lys Cys Gln Ser Leu Lys Cys Ile Cys Ala Lys Pro Cys

35 40 45

<210> 496

<211> 39

<212> PRT

<213> Pea (Pisum sativum)

<400> 496

Ser Glu Cys Asn Asp Glu Glu Cys Glu Trp Ser Cys Ile Asp Lys Gly

1 5 10 15

Phe Leu Asp Gly Val Cys Ser Pro Ser Ser Asn Gln Cys Phe Cys Val

20 25 30

Ile His Arg Val Ile Ile Arg

35

<210> 497

<211> 50

<212> PRT

<213> Pea (Pisum sativum)

<400> 497

Asn Ser Cys Gln Ser Lys Ser Ser Gln Phe Lys Gly Ile Cys Leu Ser

1 5 10 15

Asn Lys Lys Cys Ala Arg Val Cys His Leu Glu Gly Phe Leu Glu Gly

20 25 30

Gly Lys Cys Lys Gly Lys Leu Leu Lys His Cys Ser Cys Lys Lys Ser

35 40 45

Ile Cys

50

<210> 498

<211> 45

<212> PRT

<213> Pea (Pisum sativum)

<400> 498

Arg Gly Cys Lys Val Lys Ser Thr Lys Phe Arg Gly Pro Cys Ser Phe

1 5 10 15

Asp Lys Asn Cys Ala Thr Val Cys Arg Gly Glu Gly Tyr Arg Asp Gly

20 25 30

Asp Cys His Gly Leu Arg Arg Arg Cys Met Cys Leu Cys

35 40 45

<210> 499

<211> 47

<212> PRT

<213> Pea (Pisum sativum)

<400> 499

Arg Lys Cys Val Ser Gln Ser His Arg Phe His Gly Pro Cys Leu Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Leu Gly Glu Gly Phe Thr Gly Gly

20 25 30

Asp Cys His Gly Phe Arg Arg Arg Cys Phe Cys Ser Arg Phe Cys

35 40 45

<210> 500

<211> 56

<212> PRT

<213> Pea (Pisum sativum)

<400> 500

Arg His Cys Glu Ser Pro Ser His Arg Phe Lys Gly Ile Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Ala Ile Cys Gln Thr Glu Gly Phe Ser Ser Gly

20 25 30

Lys Cys Arg Gly Phe Arg Ser Arg Cys Val Cys Ile Lys His Cys His

35 40 45

His His Glu Glu Glu Val Gln Asn

50 55

<210> 501

<211> 57

<212> PRT

<213> Pea (Pisum sativum)

<400> 501

Arg Leu Cys Glu Ser Ala Ser His Arg Phe Lys Gly Ile Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Gly Ala Val Cys Gln Thr Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Ala Arg Cys Met Cys Thr Lys His Cys His

35 40 45

Gln Glu Glu Glu Ala Glu Val Lys Asn

50 55

<210> 502

<211> 57

<212> PRT

<213> Pea (Pisum sativum)

<400> 502

Met Arg Val Asp Gly Ala Arg Thr Asn Arg Met Cys Asp Ser Lys Ser

1 5 10 15

His Lys Phe Arg Gly Leu Cys Val Ser Asp Thr Asn Cys Arg Asn Val

20 25 30

Cys Gln Thr Glu Arg Phe Ser Asp Gly His Cys Arg Gly Ala Arg Arg

35 40 45

Arg Cys Tyr Cys Gln Asn Pro Cys Ile

50 55

<210> 503

<211> 57

<212> PRT

<213> Pea (Pisum sativum)

<400> 503

Arg Leu Cys Glu Ser Ala Ser His Arg Phe Lys Gly Ile Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Gly Ala Val Cys Gln Thr Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Arg Gly Leu Arg Ala Arg Cys Met Cys Thr Lys His Cys His

35 40 45

Gln Glu Glu Glu Ala Glu Val Lys Asn

50 55

<210> 504

<211> 47

<212> PRT

<213> Oak (Quercus lobata)

<400> 504

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe His Gly Gly

20 25 30

Gln Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 505

<211> 47

<212> PRT

<213> Oak (Quercus lobata)

<400> 505

Arg Val Cys Glu Ser Gln Ser His Lys Phe Lys Gly Ala Cys Met Ser

1 5 10 15

Asn His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Arg Ile Cys

35 40 45

<210> 506

<211> 50

<212> PRT

<213> China rose (Rosa chinensis)

<400> 506

Glu Asn Leu Arg Thr Cys Asp Ser Pro Ser Gly Lys Phe Lys Gly Pro

1 5 10 15

Cys Ile Ile Ser Ser Asn Cys Ala Leu Thr Cys Gln Lys Glu Gly Phe

20 25 30

Ala Gly Gly His Cys Arg Asp Phe Leu Arg Lys Cys Tyr Cys Ala Lys

35 40 45

Pro Cys

50

<210> 507

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 507

Arg Thr Cys Glu Thr Leu Ser His Lys Phe Lys Gly Thr Cys Leu Arg

1 5 10 15

Glu Ser Asn Cys Ala Ser Val Cys Gln Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 508

<211> 59

<212> PRT

<213> China rose (Rosa chinensis)

<400> 508

Gly Ile Gly Pro Asn Val Met Val Ala Glu Ala Arg Thr Cys Glu Ser

1 5 10 15

Gln Ser Leu Lys Tyr Glu Gly Met Cys Leu Arg Glu Ser His Cys Ala

20 25 30

Ser Val Cys Gln Thr Glu Gly Tyr Ser Gly Gly Asp Cys His Gly Leu

35 40 45

Tyr Ser Ile Cys Val Cys Thr Lys Asp Cys Gln

50 55

<210> 509

<211> 58

<212> PRT

<213> China rose (Rosa chinensis)

<400> 509

Gly Ile Gly Pro Asn Val Thr Val Ala Glu Ala Arg Lys Cys Glu Ser

1 5 10 15

Gln Ser His Lys Phe Ala Gly Ile Cys Leu Ile Glu Ser Asn Cys Ala

20 25 30

Ser Ile Cys Lys Thr Glu Gly Tyr Ser Gly Gly Asn Cys Gly Gly Phe

35 40 45

Arg Arg Arg Cys Phe Cys Thr Lys Asp Cys

50 55

<210> 510

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 510

Arg Lys Cys Glu Ser Gln Ser His Lys Phe Glu Gly Ile Cys Leu Lys

1 5 10 15

Gln Ser Asn Cys Ala Ser Ile Cys Lys Thr Glu Gly Tyr Ser Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Asp Cys

35 40 45

<210> 511

<211> 58

<212> PRT

<213> China rose (Rosa chinensis)

<400> 511

Gly Ile Gly Pro Asn Val Met Val Ala Glu Ala Arg Lys Cys Glu Ser

1 5 10 15

Gln Ser His Lys Phe Ala Gly Ile Cys Leu Ile Glu Ser Asn Cys Ala

20 25 30

Ser Ile Cys Lys Thr Glu Gly Tyr Ser Gly Gly Asn Cys Gly Gly Phe

35 40 45

Arg Arg Arg Cys Phe Cys Thr Lys Asp Cys

50 55

<210> 512

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 512

Arg Val Cys Glu Val Pro Ser Lys Thr Phe Ser Gly Leu Cys Phe Ser

1 5 10 15

Asp Thr His Cys Ile Val Arg Cys His Ser Glu Gly Tyr Gly Tyr Gly

20 25 30

Lys Cys Ser His Ile Leu Arg Arg Cys Arg Cys Leu Lys Ala Cys

35 40 45

<210> 513

<211> 48

<212> PRT

<213> China rose (Rosa chinensis)

<400> 513

Lys Ile Cys Glu Val Pro Ser Lys Thr Phe Lys Gly Leu Cys Val Lys

1 5 10 15

Asp Arg Asn Cys Val Val Arg Cys His Ser Glu Gly Tyr Gly Asn Gly

20 25 30

Lys Cys Ser His Val Arg Arg Gln Cys Met Cys Leu Lys Ile Cys Asp

35 40 45

<210> 514

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 514

Lys Ile Cys Glu Tyr Pro Ser Arg Thr Phe Lys Gly Leu Cys Val Lys

1 5 10 15

Asp Lys Asn Cys Val Val Arg Cys Arg Ser Glu Gly Tyr Asn Tyr Gly

20 25 30

Lys Cys Ser His Val Arg Arg Arg Cys Arg Cys Phe Lys Pro Cys

35 40 45

<210> 515

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 515

Arg Val Cys Glu Ser Gln Ser His Gly Phe His Gly Thr Cys Ile Arg

1 5 10 15

Ala His Asn Cys Ala Leu Val Cys Arg His Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 516

<211> 47

<212> PRT

<213> China rose (Rosa chinensis)

<400> 516

Arg Val Cys Glu Ser Arg Ser His Gly Phe His Gly Thr Cys Ile His

1 5 10 15

His Asn Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 517

<211> 47

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 517

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Ile Cys Val Arg

1 5 10 15

Lys Ala Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Ser Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Tyr Cys Thr Arg His Cys

35 40 45

<210> 518

<211> 42

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 518

Arg Thr Cys Glu Ser Glu Ser Ser Ser Phe Lys Gly Leu Cys Leu Arg

1 5 10 15

Asn Ala Asp Cys Tyr Ile Val Cys Gln Thr Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Ser Cys Phe Cys Thr Lys Pro Cys

35 40

<210> 519

<211> 47

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 519

Arg Thr Cys Glu Ser Glu Ser Ser Thr Phe Lys Gly Leu Cys Leu Lys

1 5 10 15

Asn Ala Asp Cys Tyr Ile Val Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys Ser Ser Phe Leu Arg Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 520

<211> 74

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 520

Val Val Cys Ser Ala Ala Ser Arg Leu Phe Ser Gly Leu Cys Phe Ser

1 5 10 15

Asp Ser Asn Cys Ser Ser Ile Cys Glu Lys Glu Gly Phe Leu Thr Gly

20 25 30

Val Cys Lys Gly Leu Gln Cys Ile Cys Gln Lys Asp Cys Gly Gly Gly

35 40 45

Asp Gly Gly Asp Pro Gly Gln Gly Gly Ala Asp Gln Ser Ala Leu Ile

50 55 60

Asn Arg Lys Leu Leu Asp Gln Leu Leu Val

65 70

<210> 521

<211> 56

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 521

Ala Ala Ala Ser Val Val Glu Glu Lys Arg Ser Cys Gln Ser Gln Ser

1 5 10 15

His Lys Phe Lys Gly Lys Cys Phe Ser Asp Lys Asn Cys Gly Ser Val

20 25 30

Cys Gln Thr Glu Gly Phe Pro Ser Gly Lys Cys His Gly Leu Arg Gln

35 40 45

Arg Cys Phe Cys Thr Arg Pro Cys

50 55

<210> 522

<211> 56

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 522

Ala Met Cys Ser Ala Pro Ser Lys Leu Phe Arg Gly Val Cys Leu Leu

1 5 10 15

Arg Ser Asn Cys Ser Ser Ile Cys Ala Thr Glu Gly Phe Leu Tyr Gly

20 25 30

Leu Cys Lys Asp Leu Lys Cys Val Cys Tyr Lys Asp Cys Asn Val Val

35 40 45

Gly Ser Gly Gly Asp Ala Gln His

50 55

<210> 523

<211> 74

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 523

Val Met Cys Ser Ala Ala Ser Arg Leu Phe Ser Gly Leu Cys Phe Ser

1 5 10 15

Asp Asn Asn Cys Ser Ala Ile Cys Glu Lys Glu Gly Phe Leu Ser Gly

20 25 30

Val Cys Lys Gly Leu Lys Cys Val Cys Gln Lys Asp Cys Asn Gly Gly

35 40 45

Gly Gly Gly Gly Gly Asn Gly Gly Gly Asp Gly Gly Asp Gln Asp Gln

50 55 60

Gly Pro Pro Ala Gln Gly Gly Gly Asp Gln

65 70

<210> 524

<211> 74

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 524

Val Met Cys Ser Ala Ala Ser Arg Leu Phe Ser Gly Leu Cys Phe Ser

1 5 10 15

Asp Asn Asn Cys Ser Ala Ile Cys Glu Lys Glu Gly Phe Leu Ser Gly

20 25 30

Val Cys Lys Gly Leu Lys Cys Val Cys Gln Lys Asp Cys Asn Gly Gly

35 40 45

Gly Gly Gly Asn Gly Gly Gly Asp Gly Gly Asp Gln Asp Gln Gly Pro

50 55 60

Pro Ala Gln Gly Gly Gly Asp Gln Ser Pro

65 70

<210> 525

<211> 73

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 525

Ala Met Cys Ser Ala Pro Ser Arg Leu Phe Arg Gly Leu Cys Phe Arg

1 5 10 15

Arg Ser Asn Cys Thr Ser Ile Cys Glu Lys Glu Gly Phe Leu Tyr Gly

20 25 30

Leu Cys Lys Gly Leu Lys Cys Val Cys Tyr Lys Asp Cys Asn Ala Ala

35 40 45

Gly Gly Gly Gly Asn Gly Gly Asp Pro Gly Gln Gly Pro Pro Asp Gln

50 55 60

Gly Gly Asp Asp Gln Pro Pro Pro Leu

65 70

<210> 526

<211> 56

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 526

Ala Ala Ala Ser Val Val Glu Glu Lys Arg Ser Cys Gln Ser Gln Ser

1 5 10 15

His Lys Phe Lys Gly Lys Cys Phe Ser Asp Lys Asn Cys Gly Ser Val

20 25 30

Cys Gln Thr Glu Gly Phe Pro Ser Gly Lys Cys His Gly Leu Arg Gln

35 40 45

Arg Cys Phe Cys Thr Arg Pro Cys

50 55

<210> 527

<211> 61

<212> PRT

<213> Nandan Ginseng (Salvia bowleyana)

<400> 527

Ala Met Cys Ser Ala Pro Ser Lys Leu Phe Arg Gly Val Cys Leu Leu

1 5 10 15

Arg Ser Asn Cys Ser Ser Ile Cys Ala Thr Glu Gly Phe Leu Tyr Gly

20 25 30

Leu Cys Lys Asp Leu Lys Cys Val Cys Tyr Lys Asp Cys Asn Val Val

35 40 45

Gly Ser Gly Ser Gly Gly Ser Gly Gly Asp Ala Gln His

50 55 60

<210> 528

<211> 48

<212> PRT

<213> Jojoba (Simmondsia chinensis)

<400> 528

Arg Ile Cys Gln Ser Gln Ser His Leu Phe Arg Gly Pro Cys Val Arg

1 5 10 15

Asn His Asn Cys Ala Ser Val Cys Gln Asn Glu Gly Phe Ser Asp Gly

20 25 30

Ser Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Ala Lys Phe Cys Pro

35 40 45

<210> 529

<211> 42

<212> PRT

<213> Chayote (Sechium edule)

<400> 529

Gln Ser His Gly Phe His Gly Ala Cys Leu Ser Asp His Asn Cys Ala

1 5 10 15

Val Val Cys Arg Asn Glu Gly Phe Ser Gly Gly Asp Cys Lys Gly Phe

20 25 30

Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40

<210> 530

<211> 48

<212> PRT

<213> Chayote (Sechium edule)

<400> 530

Arg Thr Cys Glu Ser Pro Ser Arg His Phe Lys Gly Val Cys Phe Ser

1 5 10 15

Arg Asn Asn Cys Gly His Val Cys Lys Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys Leu

35 40 45

<210> 531

<211> 48

<212> PRT

<213> Chayote (Sechium edule)

<400> 531

Arg Thr Cys Glu Ser Gln Ser His His Phe Lys Gly Leu Cys Phe Ser

1 5 10 15

Lys Asn Asn Cys Gly His Val Cys Lys Thr Glu Gly Phe His Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys His Cys Ala

35 40 45

<210> 532

<211> 50

<212> PRT

<213> Giant fir (Sequoiadendron giganteum)

<400> 532

Arg Met Cys Lys Ser Gln Ser His Thr Phe Lys Gly Tyr Cys Val Ser

1 5 10 15

Asp Ser Asn Cys Lys Asn Val Cys Arg Thr Glu Lys Phe Pro Thr Gly

20 25 30

Gly Cys Asp Phe His Val Ala Ser Arg Lys Cys Tyr Cys Tyr Lys Pro

35 40 45

Cys Ala

50

<210> 533

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 533

Arg Thr Cys Glu Ser Gln Ser His Arg Tyr Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Asn Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Ser Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Ala Lys His Cys

35 40 45

<210> 534

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 534

Arg Thr Cys Glu Ser Gln Ser His His Phe Lys Gly Asn Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Gly Ser Val Cys Arg Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 535

<211> 50

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 535

Ala Glu Ala Arg Thr Cys Glu Ser Gln Ser Asn Ser Phe Lys Gly Thr

1 5 10 15

Cys Val Arg Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe

20 25 30

Ile Gly Gly Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg

35 40 45

Asn Cys

50

<210> 536

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 536

Arg Thr Cys Glu Pro Gln Ser His Ser Phe Lys Gly Pro Cys Ser Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Gln Cys Phe Cys Thr Thr Glu Cys

35 40 45

<210> 537

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 537

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Lys Asn Cys Ala Ser Val Cys Glu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 538

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 538

Arg Val Cys Met Ser Pro Ser His Ser Tyr His Gly Pro Cys Trp His

1 5 10 15

Asp His Asn Cys Ala Ile Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Val Gly Ile Gln Leu Lys Cys Tyr Cys Thr Lys Leu Cys

35 40 45

<210> 539

<211> 47

<212> PRT

<213> Tomato (Solanum lycopersicum)

<400> 539

Arg Val Cys Ile Ser Gln Ser His Arg Tyr Arg Gly Pro Cys Val Leu

1 5 10 15

Asp His Asn Cys Ala Ile Ile Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Ile Gly Trp Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 540

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 540

Arg Thr Cys Glu Thr Gln Ser His Lys Tyr Lys Gly Pro Cys Val Arg

1 5 10 15

Lys Ser Asn Cys Ala Asn Val Cys Lys Thr Glu Gly Phe Arg Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Asn Cys

35 40 45

<210> 541

<211> 55

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 541

Gly Ile Arg Lys Thr Asp Ala Lys Leu Cys Gln Tyr His Ser Glu Thr

1 5 10 15

Phe His Gly Val Cys Val Thr Gly Asn Asn Cys Asn Arg His Cys Gln

20 25 30

Arg Glu Asn Phe Glu Gly Gly Arg Cys His Gly Ile Arg His Tyr Arg

35 40 45

Cys Val Cys Tyr Arg Thr Cys

50 55

<210> 542

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 542

Arg His Cys Glu Ser Leu Ser His Arg Phe Lys Gly Pro Cys Ala Ser

1 5 10 15

Asp Lys Asn Cys Ala Ser Ile Cys Glu Thr Glu Arg Phe Ser Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Lys Pro Cys

35 40 45

<210> 543

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 543

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Pro Cys Val Ser

1 5 10 15

Glu Lys Asn Cys Ala Ser Val Cys Glu Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Pro Cys

35 40 45

<210> 544

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 544

Arg Thr Cys Glu Ser Gln Ser His Ser Phe Lys Gly Pro Cys Ser Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asp Cys Arg Gly Leu Arg Arg Gln Cys Phe Cys Thr Thr Asp Cys

35 40 45

<210> 545

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 545

Arg Thr Cys Glu Ser Gln Ser Asn Ser Phe Lys Gly Thr Cys Val Arg

1 5 10 15

Asp Ser Asn Cys Ala Thr Val Cys Gln Thr Glu Gly Phe Ile Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 546

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 546

Arg Thr Cys Glu Ser Gln Ser His His Phe Lys Gly Asn Cys Leu Ser

1 5 10 15

Asp Thr Asn Cys Gly Ser Val Cys Arg Thr Glu Gly Phe Thr Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 547

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 547

Arg Val Cys Met Ser Pro Ser His Ser Tyr His Gly Pro Cys Trp His

1 5 10 15

Asp His Asn Cys Ala Ile Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asn Cys Val Gly Ile Gln Leu Lys Cys Tyr Cys Thr Lys Leu Cys

35 40 45

<210> 548

<211> 47

<212> PRT

<213> Pengjia (Solanum pennellii)

<400> 548

Arg Val Cys Ile Ser Gln Ser His Arg Tyr Arg Gly Pro Cys Val Leu

1 5 10 15

Asp His Asn Cys Ala Ile Ile Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys Ile Gly Trp Arg Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 549

<211> 47

<212> PRT

<213> Schrenkiella parvula

<400> 549

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Arg Gly Val Cys Val Ser

1 5 10 15

Asp Gly Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg His Cys

35 40 45

<210> 550

<211> 47

<212> PRT

<213> Schrenkiella parvula

<400> 550

Arg Thr Cys Glu Ser Gln Ser His Arg Phe Lys Gly Lys Cys Leu Ser

1 5 10 15

Asp Gly Asn Cys Gly Asn Val Cys His Asn Glu Gly Phe Gly Gly Gly

20 25 30

Lys Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 551

<211> 45

<212> PRT

<213> Schrenkiella parvula

<400> 551

Lys Thr Cys Glu Ser Pro Ser Gly Lys Tyr His Gly Val Cys Leu Asn

1 5 10 15

Ala Lys Asn Cys Ala Ser Val Cys Pro Ser Glu Gly Phe Thr Gly Gly

20 25 30

His Cys Lys Ser Leu Arg Cys Tyr Cys Ile Lys Thr Cys

35 40 45

<210> 552

<211> 63

<212> PRT

<213> Schrenkiella parvula

<400> 552

Thr Glu Met Gly Pro Ala Asp Val Glu Pro Arg Ile Cys Glu Thr Leu

1 5 10 15

Ser Lys Val Phe Lys Gly Met Cys Trp Asn Gln Asn Asn Cys Ala Ile

20 25 30

Met Cys Arg Glu Phe Glu His Phe His Ser Gly His Cys Arg Gly Phe

35 40 45

Leu Arg Arg Cys Tyr Cys Thr Lys Arg Cys Asp Gly Gly Pro Ser

50 55 60

<210> 553

<211> 52

<212> PRT

<213> Drunk butterfly flower (TARENAYA HASSLERIANA)

<400> 553

Glu Arg Gly Glu Gly Arg Met Cys Gln Ser Lys Ser His His Phe His

1 5 10 15

Arg Thr Cys Val Ser Asn His Asn Cys Ala Met Val Cys Arg Thr Glu

20 25 30

Gly Phe Ser Gly Gly His Cys Val Gly Phe Arg Arg Arg Cys Phe Cys

35 40 45

Thr Arg Leu Cys

50

<210> 554

<211> 47

<212> PRT

<213> Drunk butterfly flower (TARENAYA HASSLERIANA)

<400> 554

Arg Thr Cys Glu Ser Pro Ser His Arg Phe Lys Gly Ala Cys Leu Ser

1 5 10 15

Glu Thr Asn Cys Ala Asn Val Cys His Asn Glu Gly Phe Ile Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 555

<211> 47

<212> PRT

<213> Cocoa (Theobroma caccao)

<400> 555

Arg Thr Cys Gln Ser Gln Ser His Arg Phe Lys Gly Met Cys Met Arg

1 5 10 15

Lys Ser Asn Cys Ala Ala Val Cys Gln Thr Glu Gly Phe His Ser Gly

20 25 30

His Cys Arg Gly Phe His Arg Arg Cys Phe Cys Thr Lys His Cys

35 40 45

<210> 556

<211> 47

<212> PRT

<213> Cocoa (Theobroma caccao)

<400> 556

Arg Thr Cys Glu Ser Lys Ser His Ala Phe Lys Gly Pro Cys Leu Ser

1 5 10 15

Asp His Asn Cys Gly Leu Val Cys Arg Asn Glu Gly Phe Pro Asp Gly

20 25 30

Trp Cys Arg Gly Ile Arg Arg Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 557

<211> 47

<212> PRT

<213> Cocoa (Theobroma caccao)

<400> 557

Arg Leu Cys Glu Ser Lys Ser His Gly Phe Lys Gly Ala Cys Leu Ser

1 5 10 15

Asp His Asn Cys Gly Met Val Cys Ser Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Arg Arg Cys

35 40 45

<210> 558

<211> 47

<212> PRT

<213> Cocoa (Theobroma caccao)

<400> 558

Arg Val Cys Glu Ser Lys Ser His Lys Phe Lys Gly Pro Cys Leu Gly

1 5 10 15

Asp His Asn Cys Ala Leu Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg His Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 559

<211> 47

<212> PRT

<213> Thlaspi arvensis (THLASPI ARVENSE)

<400> 559

Arg Thr Cys Glu Ser Lys Ser His Arg Phe Lys Gly Ala Cys Leu Ser

1 5 10 15

Glu Thr Asn Cys Lys Asn Val Cys His Asn Glu Gly Phe Arg Gly Gly

20 25 30

Asn Cys Arg Gly Leu Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 560

<211> 47

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 560

Arg Lys Cys Glu Ser Lys Ser His Arg Phe Lys Gly Lys Cys Leu Ser

1 5 10 15

Glu Thr Asn Cys Lys Asn Val Cys His Asn Glu Gly Phe Thr Gly Gly

20 25 30

Asn Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Arg Asn Cys

35 40 45

<210> 561

<211> 46

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 561

Arg Thr Cys Glu Ser Ala Ser Gly Lys Phe Gln Gly Val Cys Leu Asn

1 5 10 15

Ser Lys Ser Cys Ala Ser Val Cys Gln Ser Asn Glu Gly Phe Ser Gly

20 25 30

Gly His Cys Asn Ser Leu Arg Cys Tyr Cys Thr Lys Thr Cys

35 40 45

<210> 562

<211> 47

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 562

Arg Met Cys Gln Ser Lys Ser His His Phe Lys His Met Cys Trp Ser

1 5 10 15

Asp His Asn Cys Ala Ile Ile Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Arg Cys Arg Gly Phe His Arg Arg Cys Tyr Cys Thr Arg Leu Cys

35 40 45

<210> 563

<211> 50

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 563

Gln Gln Ile Cys Lys Ala Arg Ser Ile Asn Phe Thr Ala Leu Cys Leu

1 5 10 15

Arg Trp Ser Lys Cys Lys Gln Ala Cys Leu Thr Glu Asn Phe Pro Asp

20 25 30

Gly Arg Cys Ile Ala Arg Arg Cys Phe Cys Met Thr Pro Cys His Phe

35 40 45

Leu Thr

50

<210> 564

<211> 52

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 564

Gln Gln Ile Cys Glu Ala Arg Ser Ile Asn Phe Arg Gly Met Cys Met

1 5 10 15

Lys Trp Arg Asn Cys Gln Arg Val Cys Met Ser Glu Gly Phe Pro Asp

20 25 30

Gly Arg Cys Ala Gly Phe Ile Arg Arg Cys Ile Cys Arg Lys Pro Cys

35 40 45

Pro Ile Leu Ser

50

<210> 565

<211> 100

<212> PRT

<213> Salt mustard (Eutrema salsugineum)

<400> 565

Arg Met Cys Glu Ser Ser Ser Gln Leu Phe Asn Gly Pro Cys Leu Ser

1 5 10 15

Thr Thr Asn Cys Ala Asn Ile Cys His Asn Glu Gly Phe Thr Asp Gly

20 25 30

Asp Cys Gln Gly Phe Arg Arg Arg Cys Ile Cys Thr Arg Pro Cys Thr

35 40 45

Pro Arg Gly Met Glu Arg Lys Thr Leu Gly Leu Phe Phe Met Leu Phe

50 55 60

Leu Leu Leu Ser Ala Asp Lys Tyr Tyr Phe Met Asp Ala Asp Val Ala

65 70 75 80

Leu Lys Thr Ala Glu Ala Arg Arg Cys Glu Ser Lys Ser His Lys Phe

85 90 95

Lys Gly Pro Cys

100

<210> 566

<211> 48

<212> PRT

<213> Red clover (Trifolium pratense)

<400> 566

Arg Thr Cys Glu Ser Gln Ser His Lys Phe Lys Gly Pro Cys Ala Ser

1 5 10 15

Asp His Asn Cys Ala Ser Val Cys Gln Thr Glu Arg Phe Pro Gly Gly

20 25 30

His Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys Ser

35 40 45

<210> 567

<211> 55

<212> PRT

<213> Red clover (Trifolium pratense)

<400> 567

Glu Met Val Val Glu Thr Glu Gly Arg His Cys Glu Ser Lys Ser His

1 5 10 15

Arg Phe Lys Gly Met Cys Leu Ser Asp His Asn Cys Ala Ser Val Cys

20 25 30

His Val Glu Gly Phe Pro Gly Gly Asn Cys Arg Gly Phe Arg Arg Arg

35 40 45

Cys Phe Cys Lys Lys Arg Cys

50 55

<210> 568

<211> 48

<212> PRT

<213> Red clover (Trifolium pratense)

<400> 568

Arg Lys Cys Glu Ser Lys Arg Arg Trp Ile Tyr Gly Asn Cys Leu Ser

1 5 10 15

Asp Glu Lys Cys Ala Ser Asp Cys Leu Val Lys Gly Phe Leu Phe Gly

20 25 30

Lys Cys Arg Gly Tyr Asp Asn Arg Cys Tyr Cys Lys Lys Pro Arg Cys

35 40 45

<210> 569

<211> 47

<212> PRT

<213> Tripterygium wilfordii (Tripterygium wilfordii)

<400> 569

Arg Val Cys Ala Ser Lys Ser His Tyr Tyr Lys Gly Pro Cys Leu His

1 5 10 15

Asn His Asn Cys Ala Met Val Cys Arg Asn Glu Gly Phe Ser Gly Gly

20 25 30

Lys Cys His Gly Leu Gly Arg Arg Cys Phe Cys Thr Lys Leu Cys

35 40 45

<210> 570

<211> 47

<212> PRT

<213> Tripterygium wilfordii (Tripterygium wilfordii)

<400> 570

Arg Val Cys Leu Ser Pro Ser His Lys Phe His Gly Thr Cys Val Arg

1 5 10 15

Gly His Asn Cys Ala Glu Ile Cys Arg Thr Glu Gly Phe Ser Gly Gly

20 25 30

Asp Cys His Gly Phe Arg Arg Arg Cys Met Cys Ser Arg Arg Cys

35 40 45

<210> 571

<211> 57

<212> PRT

<213> Songhao (Phtheirospermum japonicum)

<400> 571

Ala Gly Glu Glu Ala Val Gly Glu Glu Lys Lys Ala Cys Lys Tyr Lys

1 5 10 15

Ser Glu Lys Phe Trp Gly Ile Cys Ile Thr Asp Ser Lys Cys Ile Glu

20 25 30

Val Cys Arg Arg Glu Gly Phe Gln Thr Gly Asp Cys Lys Gly Leu Arg

35 40 45

Arg Arg Cys Tyr Cys Val Asn Asn Cys

50 55

<210> 572

<211> 82

<212> PRT

<213> Black beans (Vigna mungo)

<400> 572

Val Ala Ser Gly Gly Lys Asp Lys Thr Lys Lys Pro Gly Arg Tyr Ile

1 5 10 15

Ala Thr Glu Leu Glu Asn Ile Glu Thr Asn Tyr Phe Ser Asp Tyr Thr

20 25 30

Gly Phe Glu Ile Gln Ile Asp Ser Thr Asp Val Asp Asp Ala Ile Asp

35 40 45

Thr Leu Ser Glu Val Ala Ser Lys Lys Leu Val Gln Ile Leu Leu Arg

50 55 60

Phe Val Ala Leu Phe Leu Pro Val Ala Pro Ile Pro Pro Leu Pro Ile

65 70 75 80

Pro Ser

<210> 573

<211> 48

<212> PRT

<213> Maize (Zea mays)

<400> 573

Arg Thr Cys Gln Ser Gln Ser His Arg Phe Arg Gly Pro Cys Leu Arg

1 5 10 15

Arg Ser Asn Cys Ala Asn Val Cys Arg Thr Glu Gly Phe Pro Gly Gly

20 25 30

Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys His

35 40 45

<210> 574

<211> 55

<212> PRT

<213> Maize (Zea mays)

<400> 574

Arg Val Val Val Glu Glu Thr Leu Cys Leu Ser Gln Ser His Ala Phe

1 5 10 15

Lys Gly Val Cys Leu Ser Asn Thr Asn Cys Asp Asn Val Cys Lys Thr

20 25 30

Glu Lys Phe Thr Gly Gly Glu Cys Lys Met Asp Gly Val Met Arg Lys

35 40 45

Cys Tyr Cys Lys Lys Val Cys

50 55

<210> 575

<211> 49

<212> PRT

<213> Maize (Zea mays)

<400> 575

Arg His Cys Leu Ser Gln Ser His Arg Phe Lys Gly Leu Cys Met Ser

1 5 10 15

Ser Asn Asn Cys Ala Asn Val Cys Gln Thr Glu Asn Phe Pro Gly Gly

20 25 30

Glu Cys Lys Ala Glu Gly Ala Thr Arg Lys Cys Phe Cys Lys Lys Ile

35 40 45

Cys

<210> 576

<211> 55

<212> PRT

<213> Maize (Zea mays)

<400> 576

Gly Pro Val Thr Met Val Ser Ala Arg Lys Cys Glu Ser Gln Ser Phe

1 5 10 15

Arg Phe Lys Gly Pro Cys Ser Arg Asp Ala Asn Cys Ala Asn Val Cys

20 25 30

Leu Thr Glu Gly Phe Thr Gly Gly Val Cys Lys Gly Leu Arg His Arg

35 40 45

Cys Phe Cys Thr Arg Asp Cys

50 55

<210> 577

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (22)..(22)

<223> Amidation

<400> 577

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Cys Tyr Cys

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 578

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (22)..(22)

<223> Amidation

<400> 578

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Trp Phe Trp

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 579

<211> 22

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (22)..(22)

<223> Amidation

<400> 579

Gly Ala Cys Leu Lys Asn Arg His Ser Lys His Tyr Gly Phe Phe Trp

1 5 10 15

Tyr Tyr Arg His Cys Tyr

20

<210> 580

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (16)..(16)

<223> Amidation

<400> 580

Gly Arg Cys Arg Gly Phe Arg Arg Arg Cys Phe Cys Thr Thr His Cys

1 5 10 15

<210> 581

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 581

Gly Gly Arg Cys Lys Gly Phe Leu Arg Arg Phe Trp Phe Thr Arg Ile

1 5 10 15

Cys

<210> 582

<211> 17

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (17)..(17)

<223> Amidation

<400> 582

Gly Gly Arg Cys Lys Gly Phe Arg Arg Arg Trp Tyr Trp Thr Arg Ile

1 5 10 15

Cys

<210> 583

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (16)..(16)

<223> Amidation

<400> 583

Ser Gly Arg Cys Arg Ile Leu Phe Arg Cys Phe Cys Thr Lys Asn Cys

1 5 10 15

<210> 584

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (16)..(16)

<223> Amidation

<400> 584

Ser Gly Arg Cys Arg Ile Leu Phe Arg Trp Phe Trp Thr Lys Asn Cys

1 5 10 15

<210> 585

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (16)..(16)

<223> Amidation

<400> 585

Ser Gly Arg Cys Arg Ile Leu Phe Arg Trp Tyr Phe Thr Lys Asn Cys

1 5 10 15

<210> 586

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> MOD_RES

<222> (18)..(18)

<223> Amidation

<400> 586

Gly Ala Cys His Arg Gln Gly Phe Gly Phe Ala Cys Phe Cys Tyr Lys

1 5 10 15

Lys Cys

<210> 587

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(13)

<223> Xaa is any amino acid, xaa7 to Xaa13 are optionally present

<220>

<221> Variant

<222> (14)..(16)

<223> Xaa is F, W or Y

<400> 587

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

<210> 588

<211> 18

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(15)

<223> Xaa is any amino acid, xaa7 to Xaa15 are optionally present

<220>

<221> Variant

<222> (16)..(18)

<223> Xaa is F, W or Y

<400> 588

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Xaa Xaa

<210> 589

<211> 21

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(18)

<223> Xaa is any amino acid, xaa7 to Xaa18 are optionally present

<220>

<221> Variant

<222> (19)..(21)

<223> Xaa is F, W or Y

<400> 589

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Xaa Xaa Xaa Xaa Xaa

20

<210> 590

<211> 16

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(15)

<223> Xaa is any amino acid, xaa7 to Xaa15 are optionally present

<400> 590

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys

1 5 10 15

<210> 591

<211> 19

<212> PRT

<213> Artificial

<220>

<223> Synthetic

<220>

<221> Variant

<222> (2)..(2)

<223> Xaa is any amino acid

<220>

<221> Variant

<222> (4)..(18)

<223> Xaa is any amino acid, xaa7 to Xaa18 are optionally present

<400> 591

Gly Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

1 5 10 15

Xaa Xaa Cys

Claims (29)

1. A peptide comprising the amino acid sequence:

(a) A modified gamma core consensus sequence GXCX3-10(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:50)、GXCX3-10(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:587)、GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、GXCX3-9(F/W/Y)(SEQ ID NO:44)、GXCX3-8(F/W/Y/L/V/I/M)(SEQ ID NO:45)、GXCX3-10(F/W/Y/L/V/I/M)(SEQ ID NO:46)、GXCX3-8(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:47)、GXCX3-10(L/V/I/M)(F/W/Y)(L/V/I/M)(SEQ ID NO:48)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y)(F/W/Y/L/V/I/M)(SEQ ID NO:49)、GXCX3-8(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(F/W/Y/L/V/I/M)(SEQ ID NO:51)、 or GXCX3-10 (F/W/Y/L/V/I/M) (SEQ ID NO: 52), wherein the peptide further comprises a second C-terminal cysteine residue located C-terminal to the cysteine residue in the modified gamma core consensus sequence, and wherein the peptide has a net positive charge of at least 3 and a hydrophobic amino acid content of at least 18%; or (b)

(B) 1, wherein the peptide does not comprise the corresponding full-length sequence of the defensin peptide of SEQ ID NO 8, 16, 20, 23, 26, 29, 37, 38, 39, 40 or 41; optionally wherein the peptide comprises a modified gamma core consensus sequence GXCX3-8(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:33)、GXCX3-9(F/W/Y)(F/W/Y)(F/W/Y)(SEQ ID NO:34)、GXCX3-8(F/W/Y)(SEQ ID NO:43)、 or GXCX3-9 (F/W/Y) (SEQ ID NO: 44); and/or optionally wherein the C-terminal cysteine residue or C-terminal amino acid residue is amidated.

2. The peptide of claim 1, wherein the peptide comprises (i) conserved C1 and C4 cysteine residues corresponding to the N-and C-terminal cysteines of a reference defensin C-terminal peptide, wherein amino acid residues 16 and 18 of the conserved C2 and C3 cysteine residues corresponding to the reference defensin C-terminal peptide are independently substituted with tryptophan, tyrosine, phenylalanine, leucine, valine, isoleucine or methionine; and optionally wherein the defensin peptide variant has a net positive charge of at least 3 and a hydrophobic amino acid content of at least 18%; or (ii) an amino acid sequence having at least 50%, 55%, 60%, 68%, 75%, 82% or 94% sequence identity over the full length of SEQ ID NO:7,SEQ ID NO:12,SEQ ID NO:13,SEQ ID NO:14、15、17-20、22、23、25、26、28、29、31、32、42、578、579、581、582、584 or 585, wherein the peptide does not comprise the corresponding full length sequence of the defensin peptide of SEQ ID NO 8,16, 20, 23, 26, 29, 37, 38, 39, 40 or 41; and optionally wherein the peptide of (i) or (ii) comprises a disulfide bond between two cysteine residues.

3. The peptide of claim 1 or 2, wherein the peptide comprises, consists essentially of, or consists of: (i) 30 amino acid residues or less; or (ii) 15, 16 or 17 to 30 amino acid residues.

4. A composition comprising the peptide of claim 1 or 2 and an agriculturally, pharmaceutically or veterinarily practically acceptable carrier, diluent or excipient.

5. The composition of claim 4, wherein the peptide is provided at a concentration of about 0.1, 0.5, 1.0, or 5pg/ml to about 1,5, 20, 50, or 100mg/ml or at a concentration of about 0.1, 0.5, 1.0, or 5 pg/g to about 1,5, 20, 50, or 100 mg/g, and optionally wherein the composition comprises a sodium salt at a concentration of at least 100mM and/or a calcium salt at a concentration of at least 2 mM.

6. A method for preventing or reducing damage to crops by a plant pathogenic microorganism, the method comprising the step of contacting a plant, plant seed, or other part of the plant with an effective amount of the composition of claim 4.

7. The method of claim 6, wherein the plant pathogenic microorganism is fusarium species, alternaria species, verticillium species, phytophthora species, colletotrichum species, botrytis species, cercospora species, phaeobasidium species, rhizoctonia species, sclerotinia species, pythium species, phoma species, sphaerella species, top cyst species, puccinia species, septoria species, penicillium species, dichotoma species, phomopsis species, sphaeria species, powdery mildew species, white rust species, sphaeria species, achlong or stench species.

8. A medical device comprising a device and the composition of claim 4, wherein the device comprises at least one surface that is topically coated and/or impregnated with the composition.

9. The medical device of claim 8, wherein the device is a stent, catheter, contact lens, condom, patch, or septum.

10. A method for treating, preventing or inhibiting a microbial infection in a subject in need thereof, the method comprising administering to the subject an effective amount of the composition of claim 4.

11. The method of claim 10, wherein said administering comprises topical, enteral, parenteral, and/or intravenous introduction of the composition.

12. The method of claim 10, wherein the subject is a human, livestock, poultry, fish, or companion animal.

13. The method of claim 10, wherein the microbial infection is an infection of a mucosa, eye, skin and/or nail and the composition is applied to the mucosa, eye, skin and/or nail.

14. The method of claim 10, wherein the microbial infection is caused by a dermatophyte, and wherein the dermatophyte is optionally selected from the group consisting of: trichophyton rubrum, trichophyton digitatum, trichophyton purple, trichophyton aphanidermatum, trichophyton sudan, trichophyton mentagrophytes, microsporum flavum, epidermophyton floccosum and Microsporum gypseum.

15. The method of claim 10, wherein the microbial infection is caused by aspergillus, cryptococcus, penicillium, rhizopus, lepidomyces, candidiasis, lepidomyces, rhizomucor, comycete, colpitis, actinomucor, pythium, fusarium, histoplasma, or blastomycosis species.

16. The method of claim 10, wherein the microbial infection is caused by Candida species, and wherein the Candida species is Candida albicans (Candida albicans/c.albicans), candida otophylla, candida glabrata, candida parapsilosis, candida tropicalis, or Candida krusei.

17. The composition of claim 4 for use in a method of treating, preventing or inhibiting a microbial infection in a subject in need thereof.

18. The composition of claim 17, wherein the subject is a human, livestock, poultry, fish, or companion animal.

19. A plant part at least partially coated with the composition of claim 4.

20. The plant part of claim 17, wherein the part is a seed and the seed is optionally corn, soybean, wheat, rice, cotton, brassica species or tomato seed.

21. The plant part of claim, wherein the plant part is a fruit, vegetable or flower.

22. A recombinant polynucleotide comprising a polynucleotide encoding a peptide comprising the peptide of claim 1 or 2, wherein the polynucleotide encoding a first antimicrobial peptide is operably linked to a polynucleotide comprising a promoter heterologous to the polynucleotide encoding the first antimicrobial peptide, optionally wherein any amino acid substitution in the sequence increases or maintains the net positive charge of the peptide and/or increases or maintains the hydrophobicity of the peptide.

23. The recombinant polynucleotide of claim 22, wherein the recombinant polynucleotide further comprises a polynucleotide encoding: (i) A transit peptide, a vacuole targeting peptide and/or an endoplasmic reticulum targeting peptide; (ii) a plastid targeting peptide; and/or (iii) a polyadenylation or transcription termination signal, wherein the polynucleotides of (i), (ii) and/or (iii) are operably linked to the polynucleotide encoding the antimicrobial peptide.

24. The recombinant polynucleotide of claim 22, wherein the polynucleotide encoding the first antimicrobial peptide is inserted into a heterologous nucleus or plastid genome of a cell and operably linked to an endogenous promoter located in the heterologous nucleus or plastid genome.

25. A plant nuclear or plastid genome comprising a polynucleotide encoding a peptide comprising the peptide of claim 1 or 2, wherein the polynucleotide is heterologous to the nuclear or plastid genome, and wherein the polynucleotide is operably linked to an endogenous promoter of the nuclear or plastid genome.

26. A cell comprising the recombinant polynucleotide of claim 22, wherein the cell is optionally a bacterial, yeast or plant cell.

27. A plant comprising the recombinant polynucleotide of claim 22.

28. A plant part that is a plant part of the plant of claim 27, wherein the plant part comprises the recombinant polynucleotide, optionally wherein the plant part is a seed, stem, leaf, root, tuber, flower or fruit.

29. A method for producing a plant seed providing a plant resistant to infection by a plant pathogenic microorganism, the method comprising the steps of: (i) selfing or crossing the plant of claim 27; and (ii) harvesting seed comprising the recombinant polynucleotide of the plant from the selfing or crossing, thereby producing plant seed that provides a plant that is resistant to infection by a plant pathogenic microorganism.