CN117945562A - Biological degradation method of HPAM in slime water based on computer software - Google Patents
Biological degradation method of HPAM in slime water based on computer software Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 41
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- 230000015556 catabolic process Effects 0.000 title claims abstract description 27
- 239000001963 growth medium Substances 0.000 claims abstract description 58
- 238000006065 biodegradation reaction Methods 0.000 claims abstract description 21
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- 241000190932 Rhodopseudomonas Species 0.000 claims abstract description 14
- 238000013461 design Methods 0.000 claims abstract description 14
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- 241001052560 Thallis Species 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 238000002474 experimental method Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
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- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
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- 229920001817 Agar Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
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- 230000000593 degrading effect Effects 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
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- 125000000129 anionic group Chemical group 0.000 abstract description 2
- 229920002401 polyacrylamide Polymers 0.000 abstract description 2
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- 238000002955 isolation Methods 0.000 abstract 1
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- 241000222393 Phanerochaete chrysosporium Species 0.000 description 1
- 244000062594 Pithecellobium globosum Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a biodegradation method of HPAM in slime water based on computer software, in particular to the technical field of HPAM degradation by rhodopseudomonas globosa under practical simulated coal preparation environment domestication, which comprises the following steps: s1: seed preparation, S2: isolation of single colonies, S3: preparing bacterial suspension, S4: degradation test, S5: simulation calculations explore the degradation mechanism from the perspective of biological enzymes. The method adopts a traditional single-factor test to explore the influence of the composition of a culture medium and environmental factors (pH, temperature, stirring speed, degradation time and the like) on the degradation effect, and simultaneously adopts a machine learning test design to help to quickly obtain the optimal process formula so as to achieve the ideal degradation effect, lays a foundation for efficiently degrading the residual anionic polyacrylamide (HPAM) in the coal dressing circulating water, and biodegrades the HPAM in a green and safe way without causing secondary pollution to the environment.
Description
Technical Field
The invention relates to the technical field of coal dressing wastewater treatment, in particular to a biodegradation method of HPAM in slime water based on computer software.
Background
The use amount of the flocculant anionic polyacrylamide (HPAM) commonly used for coal dressing is increased year by year, and the large-scale use of the HPAM can cause the continuous accumulation of the HPAM in the coal dressing cycle, reduce the adsorption capacity of a flotation reagent on coal slime, and have adverse effects on the subsequent coal flotation. Excessive addition of HPAM can cause turbidity of water body, and influence transparency and clarity of water; the addition of excess HPAM may result in excessive flocculation, forming large flocs, increasing the difficulty of agitation and separation for subsequent processing. HPAM may cause drug residues to exceed environmental emission standards, causing environmental pollution.
The sewage treatment capacity of China is large and is influenced by regional differences, and sewage treatment schemes are difficult to unify. Therefore, the sewage to be treated can be monitored by utilizing a computer technology in all places, and the condition optimization is carried out according to the components, the content and the influence factors of pollutants in the sewage. The machine learning experimental design method introduces machine learning into experimental design to quickly find the optimal technological condition, generates a machine learning experimental design model based on a small amount of real experimental data mining to automatically generate an orthogonal experimental scheme to perform specific experimental operation, and obtains ideal/scientific experimental results through the least experimental process. Meanwhile, a machine learning degradation rate prediction model is adopted to verify the accuracy of the test scheme. And finally, adding the experimental result into a Max-flow model, and iteratively optimizing and predicting an experimental point with the optimal effect by an algorithm in the model based on the existing experimental data. The whole process does not require a large amount of data collection, but only the basic development framework data is provided at first. Because the machine learning algorithm is adopted, the optimal process formula is found at the fastest speed, and the optimization efficiency is far greater than that of the traditional test design method. Under the support of computer simulation technology, the actual operation efficiency of the sewage treatment scheme is greatly improved, and the sewage treatment quality is higher.
Although the chemical and physical treatment methods can achieve good treatment effect, the improper operation can easily bring secondary pollution; the biodegradation method can be an efficient HPAM treatment method because of mature technology, no secondary pollution and low operation cost, and can adapt to various environments. In the related research, sulfate reducing bacteria, bacillus subtilis, bacillus brown and Phanerochaete chrysosporium are found to have a degradation effect on HPAM, but rhodopseudomonas globosa is not yet found to be applied to the degradation of HPAM as a purple non-sulfur facultative aerobe gram negative capable of carrying out photosynthesis. The thallus is spherical and has flagella, and the bacterial liquid is red and suitable for growing in water and soil at room temperature. Previous studies have shown that P.globosum can degrade with HPAM as the sole nitrogen source, and does not cause secondary pollution to the environment. It has been known that rhodococcus can secrete extracellular amidase, which is involved in the HPAM degradation process. Therefore, the mechanism of enzyme degradation HPAM is explored from the viewpoint of biological enzyme conversion by adopting the technical means of degradation test and computer simulation calculation.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a biodegradation method of HPAM in slime water based on computer software, which solves the problems in the background art.
In order to achieve the above purpose, the invention is realized by the following technical scheme: a biodegradation method of HPAM in slime water based on computer software comprises the following steps:
S1: preparing seeds, namely picking a ring from a pseudomonas globosa test tube stored in a refrigerator for activation and expansion culture, inoculating the ring into an enrichment culture medium according to the volume percentage of 2%, obtaining the seeds, and domesticating the seeds;
S2: separating single colony, coating the strain culture solution acclimatized in the step S1 on a basic solid culture medium containing 100mg/lHPAM, culturing at 30 ℃ for 72 hours, and streaking and purifying the single colony on the solid culture medium;
S3: preparing bacterial suspension, inoculating the bacterial colony purified in the step S2 into an enrichment culture medium for culture, centrifuging the culture solution at 3000 r for 10min, pouring out supernatant, flushing bacteria at the bottom with sterile physiological saline, and preserving in a refrigerator at 4 ℃ for later use;
S4: the degradation test, the bacterial suspension preserved in the S3 is inoculated into a basic culture medium according to the volume percentage of 1-5%, and when the temperature is 25-40 ℃, the initial pH=5-7 and the stirring speed is 100-140 r/min, the culture is carried out for 5-8 d to carry out process optimization, a molecular simulation and artificial intelligent Max-flow platform is adopted to quickly build an orthogonal design workflow, and in addition, the DOE & ML component of the platform machine learning experiment design is adopted to carry out iterative optimization prediction on the degradation effect;
S5: the mechanism of enzymatic degradation is explored by simulation calculation, the structural model of HPAM is designed and drawn by analyzing the structural characteristics of the HPAM macromolecule by adopting computer software CHEMIDRAW, meanwhile, the three-dimensional structure of the rhodococcus amidase is displayed by adopting Pymol software, and finally, the interaction of the amidase and a substrate is explored by adopting a CDOCKER module in molecular simulation software (Discovery Studio 2020) for molecular docking.
Preferably, the strain in S1 is cultured in the medium at a temperature of 30 ℃ and an initial ph=6 at a stirring speed of 130r/min for 36 hours.
Preferably, in the step S2, the single colony growing on the solid medium is streaked and purified twice, and the inoculating needle is burnt by open fire before the second streaking, so that microorganisms on the inoculating needle are completely killed.
Preferably, the culture conditions of the culture medium in the step S3 are that the culture is performed for 48 hours at the temperature of 30 ℃ and the initial pH=6 and the stirring speed of 130 r/min.
Preferably, the strain activation method in S1 is to suck 0.3-0.4mL of strain of the rhodopseudomonas globosa test tube by a sterile straw, transplant the strain onto a slant culture medium, place the slant culture medium into a biochemical incubator, perform constant-temperature rotary shaking culture, and recover the strain to normal growth after several times of seed transfer.
Preferably, the strain expansion culture method in S1 is to add an enrichment medium into a 250mL Erlenmeyer flask, sterilize at 120-130 ℃ and then inoculate a certain amount of pure rhodopseudomonas globosa, shake-culture the medium on a gyratory shaker at 28 ℃ and 120r/min, and culture for 24h in an aerobic state.
Preferably, the strain in S1 is placed into an incubator during the day when being spread and cultivated, and the oscillator is adopted to oscillate for a period of time under the condition of insufficient illumination at night, so that oxygen required by the growth of rhodopseudomonas globosa is supplemented, the culture solution is directly observed without dyeing after 24 hours of cultivation, a large amount of thalli are active in the culture solution, the thalli are spherical, the thalli are overlapped together to form a strip shape or a sphere shape, and single thalli move in a spiral state, and are intense and irregular.
Preferably, the domestication method of the seeds comprises the following steps:
A: taking seeds prepared by the steps, inoculating 1-5% of the seeds into a domestication culture medium containing 40-80 ml of enrichment culture medium and 10-30 ml of HPAM-containing slime water according to the volume percentage, culturing for 54-72 h at the temperature of 25-40 ℃ under the conditions that the initial pH=5-7 and the stirring speed is 100-140 r/min, pouring out 10-30 ml of supernatant, adding 10-30 ml of HPAM-containing slime water, and continuously culturing for 4-7 d;
B: inoculating 1-5% of the bacterial liquid acclimated in the first stage into an acclimating culture medium containing 100-150 ml of HPAM-containing slime water and 30-50 ml of enrichment culture medium according to the volume percentage, culturing for 54-72 h at the temperature of 25-40 ℃ under the conditions that the initial pH=5-7 and the stirring speed is 100-140 r/min, pouring out 30-50 ml of supernatant, adding 30-50 ml of HPAM-containing slime water, and continuously culturing for 7-10 d;
Preferably, the enrichment medium in S1 is formed by mixing raw materials of NH 4Cl 1g、K2HPO4 0.2g、Na Cl 0.5g、NaHCO3 1g、CH3 COONa 2g, yeast extract 1g, mnCl 2·4H2 O0.3 g, trace elements 5ml, agar 20g, 1000ml deionized water and the like.
The invention provides a biodegradation method of HPAM in slime water based on computer software. The beneficial effects are as follows:
The biodegradation method of HPAM in slime water based on computer software is environment-friendly and low in production cost, and particularly relates to a biodegradation method of HPAM by utilizing rhodopseudomonas globosa domesticated under simulated actual slime water environment, which adopts a single factor test to perform preliminary condition optimization exploration on the composition of a culture medium and environmental factors (pH, temperature, stirring speed, degradation time and the like), adopts a Max-flow platform orthogonal design assembly and a machine learning experiment design (DOE & ML) assembly to perform degradation effect iterative process optimization prediction so as to obtain better degradation effect, lays a foundation for efficiently degrading residual HPAM in the slime water, and is a green and safe method for degrading the HPAM without causing secondary pollution to the environment.
Drawings
FIG. 1 is a schematic flow chart of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1:
a biodegradation method of HPAM in slime water based on computer software comprises the following steps:
S1: preparing seeds, namely picking a ring from a pseudomonas globosa test tube stored in a refrigerator for activation and expansion culture, inoculating the ring into an enrichment culture medium according to the volume percentage of 2%, obtaining the seeds, and domesticating the seeds;
S2: separating single colony, coating the strain culture solution acclimatized in the step S1 on a basic solid culture medium containing 100mg/lHPAM, culturing at 30 ℃ for 72 hours, and streaking and purifying the single colony on the solid culture medium;
S3: preparing bacterial suspension, inoculating the bacterial colony purified in the step S2 into an enrichment culture medium for culture, centrifuging the culture solution at 3000 r for 10min, pouring out supernatant, flushing bacteria at the bottom with sterile physiological saline, and preserving in a refrigerator at 4 ℃ for later use;
S4: the degradation test, the bacterial suspension preserved in the S3 is inoculated into a basic culture medium according to the volume percentage of 1-5%, molecular simulation and an artificial intelligent Max-flow platform are adopted to quickly build an orthogonal design workflow when the culture is carried out for 5 days for process optimization at the temperature of 25 ℃ under the conditions of initial pH=5 and the stirring speed of 100r/min, and in addition, the DOE & ML component of the platform machine learning experiment design is adopted to carry out iterative optimization prediction on the degradation effect;
S5: the mechanism of enzymatic degradation is explored by simulation calculation, the structural model of HPAM is designed and drawn by analyzing the structural characteristics of the HPAM macromolecule by adopting computer software CHEMIDRAW, meanwhile, the three-dimensional structure of the rhodococcus amidase is displayed by adopting Pymol software, and finally, the interaction of the amidase and a substrate is explored by adopting a CDOCKER module in molecular simulation software (Discovery Studio 2020) for molecular docking.
The strain in S1 is cultured in a culture medium for 36 hours under the conditions that the temperature is 30 ℃, the initial pH=6 and the stirring speed is 130 r/min; in the step S2, the single colony growing is streaked and purified on a solid culture medium for two times, and before streaking for the second time, the inoculating needle is burnt by open fire, so that microorganisms on the inoculating needle are killed completely; the culture condition of the culture medium in the step S3 is that the culture is carried out for 48 hours at the temperature of 30 ℃ and the initial pH=6 and the stirring speed of 130 r/min; the strain activation method in the step S1 is that a sterile straw is used for sucking the strain of a 0.3mL rhodopseudomonas globosa test tube, the strain is transplanted onto a slant culture medium and is placed in a biochemical incubator for constant-temperature rotary shaking culture, and the strain is recovered to normally grow after a plurality of times of seed transfer; the strain expansion culture method in the S1 is that an enrichment culture medium is added into a 250ml Erlenmeyer flask, a certain amount of pure rhodopseudomonas globosa is inoculated after sterilization at 120 ℃, the culture medium is subjected to shaking culture on a gyratory shaker at the temperature of 28 ℃ and 120r/min, and the culture is carried out for 24 hours in an aerobic state.
Example 2:
a biodegradation method of HPAM in slime water based on computer software comprises the following steps:
S1: preparing seeds, namely picking a ring from a pseudomonas globosa test tube stored in a refrigerator for activation and expansion culture, inoculating the ring into an enrichment culture medium according to the volume percentage of 2%, obtaining the seeds, and domesticating the seeds;
S2: separating single colony, coating the strain culture solution acclimatized in the step S1 on a basic solid culture medium containing 100mg/lHPAM, culturing at 30 ℃ for 72 hours, and streaking and purifying the single colony on the solid culture medium;
S3: preparing bacterial suspension, inoculating the bacterial colony purified in the step S2 into an enrichment culture medium for culture, centrifuging the culture solution at 3000 r for 10min, pouring out supernatant, flushing bacteria at the bottom with sterile physiological saline, and preserving in a refrigerator at 4 ℃ for later use;
S4: the degradation test, the bacterial suspension preserved in the S3 is inoculated into a basic culture medium according to the volume percentage of 1-5%, molecular simulation and an artificial intelligent Max-flow platform are adopted to quickly build an orthogonal design workflow when the culture is carried out for 8d for process optimization at the temperature of 40 ℃ under the conditions of initial pH=7 and the stirring speed of 140r/min, and in addition, the DOE & ML component of the platform machine learning experiment design is adopted to carry out iterative optimization prediction on the degradation effect;
S5: the mechanism of enzymatic degradation is explored by simulation calculation, the structural model of HPAM is designed and drawn by analyzing the structural characteristics of the HPAM macromolecule by adopting computer software CHEMIDRAW, meanwhile, the three-dimensional structure of the rhodococcus amidase is displayed by adopting Pymol software, and finally, the interaction of the amidase and a substrate is explored by adopting a CDOCKER module in molecular simulation software (Discovery Studio 2020) for molecular docking.
The strain in S1 is cultured in a culture medium for 36 hours under the conditions that the temperature is 30 ℃, the initial pH=6 and the stirring speed is 130 r/min; in the step S2, the single colony growing is streaked and purified on a solid culture medium for two times, and before streaking for the second time, the inoculating needle is burnt by open fire, so that microorganisms on the inoculating needle are killed completely; the culture condition of the culture medium in the step S3 is that the culture is carried out for 48 hours at the temperature of 30 ℃ and the initial pH=6 and the stirring speed of 130 r/min; the strain activation method in the step S1 is that a sterile straw is used for sucking the strain of a 0.4mL rhodopseudomonas globosa test tube, the strain is transplanted onto a slant culture medium and is placed in a biochemical incubator for constant-temperature rotary shaking culture, and the strain is recovered to normally grow after a plurality of times of seed transfer; the strain expansion culture method in the S1 is that an enrichment culture medium is added into a 250ml Erlenmeyer flask, a certain amount of pure rhodopseudomonas globosa is inoculated after sterilization at 130 ℃, the culture medium is subjected to shaking culture on a gyratory shaker at the temperature of 28 ℃ and 120r/min, and the culture is carried out for 24 hours in an aerobic state.
Example 3:
The domestication method of the seeds comprises the following steps:
A: taking seeds prepared in the steps, inoculating 5% of the seeds into a domestication culture medium containing 80ml of enrichment culture medium and 30ml of HPAM-containing slime water according to the volume percentage, culturing for 72 hours at the temperature of 40 ℃ under the conditions that the initial pH=7 and the stirring speed is 140r/min, pouring out 30ml of supernatant, adding 30ml of HPAM-containing slime water, and continuously culturing for 7d;
B: inoculating 5% of the bacterial liquid acclimated in the first stage into an acclimating culture medium containing 150ml of HPAM-containing slime water and 50ml of enrichment culture medium according to the volume percentage, culturing for 72h at the temperature of 40 ℃ under the conditions that the initial pH=7 and the stirring speed is 140r/min, pouring out 50ml of supernatant, adding 50ml of HPAM-containing slime water, and continuously culturing for 10d;
The enrichment culture medium in the S1 is formed by mixing raw materials of NH 4Cl 1g、K2HPO4 0.2g、Na Cl 0.5g、NaHCO3 1g、CH3 COONa 2g, yeast extract 1g, mnCl 2·4H2 O0.3 g, trace elements 5ml, agar 20g, 1000ml deionized water and the like.
The biodegradation method of HPAM in slime water based on computer software is environment-friendly and low in production cost, and particularly relates to a biodegradation method of HPAM by utilizing rhodopseudomonas globosa domesticated under simulated actual slime water environment, which adopts a single factor test to perform preliminary condition optimization exploration on the composition of a culture medium and environmental factors (pH, temperature, stirring speed, degradation time and the like), adopts a Max-flow platform orthogonal design assembly and a machine learning experiment design (DOE & ML) assembly to perform degradation effect iterative process optimization prediction so as to obtain better degradation effect, lays a foundation for efficiently degrading residual HPAM in the slime water, and is a green and safe method for degrading the HPAM without causing secondary pollution to the environment.
While the fundamental and principal features of the invention and advantages of the invention have been shown and described, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (9)
1. A biodegradation method of HPAM in slime water based on computer software is characterized in that: the method comprises the following steps:
S1: preparing seeds, namely picking a ring from a pseudomonas globosa test tube stored in a refrigerator for activation and expansion culture, inoculating the ring into an enrichment culture medium according to the volume percentage of 2%, obtaining the seeds, and domesticating the seeds;
S2: separating single colony, coating the strain culture solution acclimatized in the step S1 on a basic solid culture medium containing 100mg/l HPAM, culturing at 30 ℃ for 72 hours, and streaking and purifying the single colony on the solid culture medium;
S3: preparing bacterial suspension, inoculating the bacterial colony purified in the step S2 into an enrichment culture medium for culture, centrifuging the culture solution at 3000 r for 10min, pouring out supernatant, flushing bacteria at the bottom with sterile physiological saline, and preserving in a refrigerator at 4 ℃ for later use;
S4: the degradation test, the bacterial suspension preserved in the S3 is inoculated into a basic culture medium according to the volume percentage of 1-5%, and when the temperature is 25-40 ℃, the initial pH=5-7 and the stirring speed is 100-140 r/min, the culture is carried out for 5-8 d to carry out process optimization, a molecular simulation and artificial intelligent Max-flow platform is adopted to quickly build an orthogonal design workflow, and in addition, the DOE & ML component of the platform machine learning experiment design is adopted to carry out iterative optimization prediction on the degradation effect;
S5: the mechanism of enzymatic degradation is explored by simulation calculation, the structural model of HPAM is designed and drawn by analyzing the structural characteristics of the HPAM macromolecule by adopting computer software CHEMIDRAW, meanwhile, the three-dimensional structure of rhodococcus amidase is displayed by adopting Pymol software, and finally, the interaction between amidase and a substrate is explored by adopting CDOCKER modules in molecular simulation software (Discovery Studio 2020) for complex model construction.
2. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the strain in S1 is cultured in a culture medium for 36h under the conditions that the temperature is 30 ℃, the initial pH=6 and the stirring speed is 130 r/min.
3. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: in the step S2, the single colony growing on the solid culture medium is streaked and purified twice, and before streaking for the second time, the inoculating needle is burnt by open fire, so that microorganisms on the inoculating needle are killed completely.
4. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the culture conditions of the culture medium in the step S3 are that the culture is carried out for 48 hours at the temperature of 30 ℃ and the initial pH=6 and the stirring speed of 130 r/min.
5. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the strain activation method in the S1 is to firstly suck the strain of the 0.3-0.4mL rhodopseudomonas globosa test tube by using a sterile straw, transplant the strain onto a slant culture medium, and put the strain into a biochemical incubator for constant-temperature rotary shaking culture, and recover the normal growth of the strain after a plurality of times of seed transfer.
6. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the strain expansion culture method in the S1 is that an enrichment culture medium is added into a 250ml Erlenmeyer flask, a certain amount of pure rhodopseudomonas globosa is inoculated after sterilization at the temperature of 120-130 ℃, the culture medium is subjected to shake culture on a gyratory shaker at the temperature of 28 ℃ and 120r/min, and the culture is carried out for 24 hours in an aerobic state.
7. The method for biodegradation of HPAM in slime water based on computer software of claim 6, wherein: the strain in the S1 is placed into an incubator in the daytime during the expansion culture, the oscillator is adopted to oscillate for a period of time under the condition of insufficient illumination at night, oxygen required by the growth of rhodopseudomonas globosa is supplemented, the culture solution is directly observed after 24 hours of culture, a large amount of thalli are active in the culture solution, the thalli are spherical, the thalli are overlapped together to form a strip shape or a sphere shape, and single thalli move in a spiral state, and are severe and irregular.
8. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the domestication method of the seeds comprises the following steps:
A: taking seeds prepared by the steps, inoculating 1-5% of the seeds into a domestication culture medium containing 40-80 ml of enrichment culture medium and 10-30 ml of HPAM-containing slime water according to the volume percentage, culturing for 54-72 h at the temperature of 25-40 ℃ under the conditions that the initial pH=5-7 and the stirring speed is 100-140 r/min, pouring out 10-30 ml of supernatant, adding 10-30 ml of HPAM-containing slime water, and continuously culturing for 4-7 d;
b: taking bacterial liquid acclimated in the first stage, inoculating 1-5% by volume of bacterial liquid into an acclimating culture medium containing 100-150 ml of HPAM-containing slime water and 30-50 ml of enrichment culture medium, culturing for 54-72 h at the temperature of 25-40 ℃ under the condition that initial pH=5-7 and stirring speed is 100-140 r/min, pouring out 30-50 ml of supernatant, adding 30-50 ml of HPAM-containing slime water, and continuously culturing for 7-10 d.
9. The method for biodegradation of HPAM in slime water based on computer software of claim 1, wherein: the enrichment culture medium in the S1 is formed by mixing raw materials of NH 4Cl 1g、K2HPO4 0.2g、Na Cl0.5g、NaHCO3 1g、CH3 COONa 2g, yeast extract 1g, mnCl 2·4H2 O0.3 g, trace elements 5ml, agar 20g, 1000ml deionized water and the like.
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