CN117925440A - Bacillus paratungensis CQ1 and application thereof - Google Patents

Abstract

The invention belongs to the technical field of microorganisms, and particularly provides a pair of bacillus licheniformis CQ1, wherein the pair of bacillus licheniformis CQ1 is preserved in China Center for Type Culture Collection (CCTCC) NO: M2023491, and a 16S rDNA sequence is shown as SEQ ID NO. 1. The strain has salt tolerance, can secrete protease, ferrite and auxin, and has growth promoting effect on tomato seeds and tomato plants. The bacillus paratungensis CQ1 and the secondary metabolite thereof have broad-spectrum antibacterial property and can inhibit the growth of pathogenic bacteria.

Description

Bacillus paratungensis CQ1 and application thereof

Technical Field

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus vice CQ1 and application thereof.

Background

In agricultural production, some fungi have a disease effect on crops, which causes great damage to agricultural production. At present, the plant diseases are controlled in production mainly by spraying chemical bactericides besides selecting fine varieties and improving cultivation measures. Most of the used chemical bactericides have different degrees of toxic action on human bodies and animals, harmful ingredients remained on edible parts of plants can cause potential threat to human health, and moreover, some chemical pesticides are difficult to decompose and can accumulate in an ecological system for a long time, so that the environment is polluted, and the sustainable development of society and economy is not facilitated. Moreover, existing chemical pesticides are not completely effective against certain plant diseases. Therefore, while efforts are made to develop new generation chemical pesticides, there is a need for intensive research and development of biological source pesticides that are efficient, safe, economical, and have good environmental compatibility.

Microorganisms and their metabolites play a very important role in biological control of plant diseases, and therefore, how to develop and correctly use biological bactericides is a first choice for developing organic and green agriculture.

Disclosure of Invention

The invention aims to provide a plant disease biocontrol strain.

Therefore, the invention provides the bacillus licheniformis CQ1, wherein the bacillus licheniformis CQ1 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2023491.

Specifically, the 16S rDNA sequence of the Bacillus paratyphenius CQ1 is shown in SEQ ID NO. 1.

Specifically, the Bacillus paratlicheniformis CQ1 can secrete auxin, protease and ferrite.

Specifically, the Bacillus paratungensis CQ1 can inhibit pathogenic bacteria.

Specifically, the pathogenic bacteria include Alternaria solani, alternaria brassicae, alternaria zeae, phytophthora cucurbitae, sclerotinia lettuce, fusarium solani, curvularia lunata, botrytis cinerea, pitaya canker, and Citrus anthracis.

In particular, the Bacillus paratungensis CQ1 can promote plant growth.

Specifically, the plant is tomato.

The invention also provides a Bacillus paratungensis CQ1 metabolite, which is prepared by the following steps: inoculating the bacillus paralicheniformis CQ1 into an LB liquid culture medium for culture, centrifuging the cultured bacterial liquid, and filtering bacterial bodies to obtain a sterile fermentation liquid, namely the metabolite.

Specifically, the above metabolites are capable of inhibiting pathogenic bacteria.

Specifically, the pathogenic bacteria include Botrytis cinerea, fusarium solani, fusarium oxysporum, alternaria solani, alternaria alternata, pitaya canker and strawberry anthracnose.

Compared with the prior art, the invention has the following advantages and beneficial effects:

The salt tolerance concentration of the Bacillus paratyphilis CQ1 is less than or equal to 14%, the protease, the ferrite and the auxin IAA can be secreted, and the Bacillus paratyphilis CQ1 has growth promotion effect on tomato seeds and tomato plants. The bacillus paratungensis CQ1 and the secondary metabolite thereof have broad-spectrum antibacterial property and antibacterial effect on various plant pathogenic bacteria.

The present invention will be described in further detail with reference to the accompanying drawings.

Drawings

FIG. 1 shows the bacteriostatic effect of Bacillus paratlicheniformis CQ1 in example 1 of the invention on Fusarium solani.

FIG. 2 is a schematic diagram showing morphological characteristics of Bacillus paratlicheniformis CQ1 in example 2 of the present invention.

FIG. 3 is a schematic representation of the gram stain results of Bacillus paralicheniformis CQ1 in example 2 of the present invention.

FIG. 4 is a graph showing the results of experiments on salt tolerance concentration of Bacillus paratlicheniformis CQ1 in example 3 of the present invention.

FIG. 5 is a graph showing the results of detection of IAA (A), protease (B) and sideBacillus licheniformis CQ1 in example 3 of the present invention.

FIG. 6 is a graph showing the results of a broad-spectrum bacteriostasis experiment of Bacillus paratlicheniformis CQ1 in example 4 of the present invention; A. alternaria solani; B. alternaria brassicae; C. the corn leaf spot germ; D. phytophthora cucurbitae; E. sclerotinia sclerotiorum; F. fusarium solani (L.) of Lycopersicon esculentum; G. curvularia lunata; H. botrytis cinerea; I. dragon fruit canker pathogen; J. citrus anthracnose germ.

FIG. 7 is a graph showing the results of bacteriostasis experiments on the metabolites of Bacillus paratungstate CQ1 in example 5 of the present invention; A. the strawberry gray mold germ; B. fusarium solani of Jiujiujiu shed; C. watermelon fusarium wilt bacteria; D. tea leaf spot germ; E. alternaria solani; F. citrus anthracnose bacteria; G. dragon fruit canker pathogen; H. strawberry anthracnose pathogen; I. pepper southern blight germ.

FIG. 8 is a schematic representation of tomato seed radicle length in example 6 of the present invention.

FIG. 9 is a schematic representation of tomato plant growth in example 7 of the present invention.

FIG. 10 is a schematic representation of the root system of each tomato plant in example 7 of the present invention.

Detailed Description

The technical solutions of the present invention will be clearly and completely described in the following examples, and it is obvious that the described examples are only some examples of the present invention, but not all examples. Although representative embodiments of the present invention have been described in detail, those skilled in the art to which the invention pertains will appreciate that various modifications and changes can be made without departing from the scope of the invention. Accordingly, the scope of the invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.

The Bacillus paratlicheniformis CQ1 of the present invention and its effects were investigated by the following specific examples.

Example 1:

The embodiment provides a separation and screening method of bacillus vice CQ1, which comprises the following steps:

The method comprises the steps of collecting about 10cm of soil of rhizosphere of crops such as tomatoes, peppers, rice, soybeans, pear trees, oranges and tangerines, strawberries, corns, young plants and the like collected in a Cai Dian area of Wuhan City in 10 months 2022, collecting the soil by a 3-point sampling method, drying, grinding and sieving the soil.

5G of the previously crushed soil was weighed, poured into a sterile conical flask, 45mL of sterile water was added, shaken for 30min by a shaker, and allowed to stand until delamination. 1mL of the supernatant was taken, 9mL of sterile water was added at a concentration of 10 ^-1, and the mixture was serially diluted to 10 ^-3,10^-4,10^-5. 0.1mL of the solution with the concentration of 10 ^-3,10^-4,10^-5 is uniformly coated on a flat plate and repeated for 3 times.

The coated plate is inverted and incubated for 48 hours at a constant temperature of 28 ℃. Colonies grow on the plate, colonies with different forms are randomly selected, purified and cultured, and then the colonies are subjected to plate confrontation with pathogenic bacteria. The culture medium is LB solid culture medium (peptone 10g/L, yeast extract powder 5g/L, sodium chloride 10g/L, agar powder 15-20g/L, and sterilizing at 121deg.C for 30 min).

Fusarium solani is taken as indicator bacteria, pathogenic bacteria hypha is selected from a test tube, inoculated in PDA solid culture medium, and cultured in a constant temperature incubator at 25 ℃ for 7d. Sterilizing with a puncher with diameter of 5mm, making into round fungus blocks (phi 5 mm), and storing in a low-temperature refrigerator with temperature of 4deg.C for use. Fusarium solani (phi 5 mm) is placed on one side of a PDA solid culture medium, the other side is inoculated with the bacteria after screening and purification, and the bacteria are cultured for 4-7d in a constant temperature cabinet at 25 ℃ to observe whether the bacteria inhibition effect is generated. Each group was repeated 3 times.

One strain of the strains separated from the soil has a good antibacterial effect on Fusarium solani, and is named as CQ1, the width of a primary opposite antibacterial zone is 3.05mm, and the antibacterial effect is shown in figure 1.

Example 2:

This example identifies the strains screened in example 1.

1. Morphological observation

The bacterial suspension cultured for 48 hours is taken, coated on LB solid medium with proper concentration, placed in a constant temperature incubator at 28 ℃ for culturing for 48 hours, and observed in color and morphology according to Berger's bacteria identification manual (eighth edition).

Gram staining step: a proper amount of bacterial liquid is taken from a liquid transferring gun and uniformly smeared on one surface of a glass slide, and the glass slide is baked and fixed by using an alcohol lamp outer flame; naturally cooling, dropwise adding crystal violet dye solution at the fixed position, standing for 1min, and cleaning with sterile water; dripping iodine solution into the counterstain, standing for 1min, and washing with water; dripping 95% ethanol decolorized onto glass slide, shaking the glass slide slightly, repeating for several times until decolorizing is clean, and washing with water; the tomato O dye liquor is dyed, kept stand for 1min, washed by water, and the filter paper absorbs water.

The slide glass is placed under an oil lens, the shape, the color and the like of bacteria are observed, blue-violet is gram-positive bacteria, and red is gram-negative bacteria.

On LB medium, strain CQ1 was milky white, opaque, dry on the surface, and irregularly serrated on the edge, as shown in FIG. 2. The strain CQ1 was gram-stained bluish violet and gram-positive as shown in FIG. 3.

2. Physiological and biochemical characterization

Methyl red M.R test: one test tube is inoculated with bacteria, the other test tube is not inoculated with the control, the test tube is cultured for 48 hours at the temperature of 28 ℃, and a methyl red indicator is dripped, if the color of the culture medium is red, the test tube is positive, and if the culture medium is yellow, the test tube is negative. The test was repeated 3 times.

V.p. test: one test tube is inoculated with bacteria, the other test tube is not inoculated with control, the test tube is cultured for 48 hours at the temperature of 28 ℃, then 40% NaOH with equal amount is added, and a small amount of creatine raw powder is added, and the test tube waits for 10 minutes or longer until the test tube turns red to be positive. The test was repeated 3 times.

Contact enzyme test: picking and culturing thalli for 48 hours, smearing the thalli on a glass slide, then dripping 10% H2O2, and observing whether small bubbles are generated; the positive air bubbles were present and the negative air bubbles were absent. The test was repeated 3 times.

Aerobic or anaerobic test: drying under sterile air until semi-solid still flows, adding 0.1mL of fresh bacterial liquid at about 45 ℃, fully mixing, airing, sealing, and culturing for 3d at 28 ℃; the growth conditions are three, and the aerobic growth is only on the surface; anaerobic only grows at the bottom; facultative anaerobism can grow. The test was repeated 3 times.

Gelatin liquefaction test: pouring gelatin liquefaction culture medium into test tube, tilting to solidify, inoculating strain on one branch, culturing at 28deg.C for 3-14d without inoculating strain on the other branch, and observing gelatin liquefaction condition. If the gelatin in the test tube is in a flowing liquid, the test tube is positive; if gelatin remains solid in the tube, it is negative. The test was repeated 3 times.

Starch hydrolysis test: selecting strains, inoculating the strains to a starch hydrolysis culture medium, culturing at 28 ℃ for 48 hours, and obtaining culture medium colonies; and (3) dripping iodine solution, waiting until the blue black color around the bacterial colony fades, and if the bacterial colony is still blue black, the starch is hydrolyzed positively, and if the bacterial colony is still blue black, the starch is not hydrolyzed, and the bacterial colony is negative. The test was repeated 3 times.

Lipase: after Tween801% was added to LB medium, the strain was streaked on the medium and cultured at 28℃to see whether or not there was a positive halo production and a negative halo production every day. The test was repeated 3 times.

Citrate utilization: the strain is streaked on a citrate culture medium, cultured for 48 hours at the temperature of 28 ℃, and then whether the culture medium changes color or not is observed, the culture medium does not change color to be negative, and the culture medium changes color to be positive. The test was repeated 3 times.

The physiological and biochemical identification of CQ1 is carried out, and the identification results are shown in Table 1.

TABLE 1CQ1 physiological and Biochemical identification

The "-" indicates negative, and "+" indicates positive

3. 16S rDNA sequence analysis

DNA extraction A TSINGKE plant DNA extraction kit (general type) (cat# TSP 101) was used.

The PCR amplification is carried out by adopting the universal primers for identifying strains in the table 2, and the amplified products are analyzed by agarose gel electrophoresis to determine whether the PCR product bands are consistent with the target size, single or have dragging bands.

TABLE 2 primers for identifying strains

After the PCR product is detected to be qualified, the target band is cut for purification and recovery, and Sanger sequencing is carried out by using the recovered product.

The sequencing result comparison analysis adopts the following method:

(1) Sanger sequencing results were spliced using software Contigexpress and the two-terminal inaccurate portions were removed.

(2) The splice sequences were blastn (latest version v 2.13) aligned in batches to the nucleic acid database. Wherein the nucleic acid database selects the newest version of the nt library by blastn with the nt library

And (3) comparing to obtain AccessionNumber numbers of homologous sequences, and identifying and annotating species.

The 16S rDNA sequence of the strain CQ1 is shown in SEQ ID NO. 1.

SEQ ID NO.1：

GTCTTACGCTGTGGCGCGAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCG CACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAGGGTAAGCCGTAAAATTAAGATGTTTATT

TABLE 3 identification results

The strain CQ1 is identified as Bacillus paratlicheniformis Bacillus paralicheniformis through morphological observation, physiological and biochemical index identification and 16S rDNA sequence analysis of the CQ1; the obtained product is sent to China center for type culture Collection (China, type culture Collection, and the like) at 2023, 4 and 9 days, and is named after classification: bacillus paralicheniformis CQ1; the preservation number is: CCTCC NO: M2023491; location: chinese, university of martial arts, martial arts.

Example 3:

This example investigated the salt tolerance and auxin IAA, protease and ferrite secretion assays of Bacillus paralicheniformis CQ 1.

1. Salt resistance: preparing an LB culture medium, inoculating the strain, and culturing at 30 ℃ for 24 hours at 200r/min to obtain seed liquid; respectively adding 2%, 4%, 6%, 8%, 10%, 12% and 14% sodium chloride into LB culture medium, respectively inoculating 1mL seed solution with LB culture medium as control; culturing at 30deg.C for 48 hr/min at 200r, and observing turbidity degree of the culture medium to confirm growth of sterile strain.

As shown in FIG. 4, the strain CQ1 is resistant to salt by less than or equal to 14%.

2. IAA secretion assay: 50mL of LB liquid medium is respectively prepared, 1% of L-tryptophan is added, and then biocontrol bacteria Bs wy-1 and Bs-9 are inoculated, and the temperature is kept at 28 ℃, and the culture is carried out at 200rpm/min for 48 hours. And respectively taking the bacterial solutions in a centrifuge tube, and centrifuging at 10000rpm for 20min. 1mL of the supernatant was taken, 2mL Salkowaski reagent was added, followed by two drops of orthophosphoric acid. And (3) carrying out water bath on the mixed solution at 28 ℃ for 2 hours, observing whether the solution turns red, and if so, indicating that the biocontrol bacteria can secrete IAA. The test was repeated 3 times.

As shown in FIG. 5A, the medium turned red after the water bath, indicating that CQ1 was capable of producing IAA.

3. Protease detection: peptone 0.1%; beef extract 0.5%; sodium chloride 0.5%; agar 2%, distilled water 1L; pH 7.00-7.2; skimmed milk powder 0.3% (112 ℃ C. Sterilized alone). The medium was then inoculated with a strain which, if a transparent loop was produced, demonstrated the ability to secrete proteases.

And (3) detecting the siderophores: CAS medium; sterilizing at 116 deg.C for 20min. The medium was then inoculated with a strain which, if a transparent loop was produced, demonstrated the ability to secrete ferrites.

As shown in FIGS. 5B-5C, both the protease culture medium and the siderophore culture medium had transparent circles, indicating that CQ1 was capable of producing proteases and siderophores.

Example 4:

This example investigated the broad-spectrum bacteriostasis of Bacillus paralicheniformis CQ 1.

As shown in FIG. 6 and Table 4, CQ1 has antibacterial effect on the following pathogenic bacteria, the lettuce sclerotinia with the best antibacterial effect, and the Botrytis cinerea and the dragon fruit canker, wherein the difference between the three is not obvious, and the antibacterial effect is more than or equal to 40%. The CQ1 has the biocontrol potential, has different inhibition effects on different pathogenic bacteria, and has broad-spectrum antibacterial effect.

Table 4 broad-spectrum antibacterial Properties of CQ1

Note that: "±" means mean ± standard deviation, and different lowercase letters after the numbers means that the Duncan new complex polar difference method is used for the difference display at the level of 0.05; CK is a blank.

Example 5:

This example investigated the bacteriostatic effect of the secondary Bacillus licheniformis CQ1 metabolite.

Preparing a sterile fermentation liquid: dipping a small amount of CQ1 strain which is streaked on a flat plate for 48 hours into LB liquid culture medium by using an inoculating loop, and culturing for 48 hours at the temperature of 30 ℃ by using a shaking table of 180 r/min; centrifuging the cultured bacterial liquid 9000r/min for 10min, passing through a 0.22 μm sterile filter membrane, and placing the sterile fermentation liquid in a refrigerator at 4 ℃ for standby.

And (3) punching holes on the PDA solid culture medium equidistantly by using a sterile gun head, adding 100 mu L of sterile fermentation filtrate, inoculating pathogenic bacteria cakes in the middle of a flat plate, culturing for 4-7d, and measuring the width of a bacteriostatic zone by using a vernier caliper.

As shown in Table 5 and FIG. 7, the sterile fermentation filtrate of CQ1 has different degrees of antibacterial effect on the gray mold of strawberry, the fusarium of tomato, the fusarium wilt of watermelon, the leaf spot of tea, alternaria solani, citrus anthracnose, dragon fruit canker and strawberry anthracnose, has the best antibacterial effect on Alternaria solani, has the antibacterial band width of 10.19mm, has obvious difference compared with other, and has the second that the difference between the gray mold of strawberry and the fusarium wilt of tomato of 6.51mm and 5.57mm is not obvious, and the antibacterial effect on the pepper southern blight is not ideal.

TABLE 5 bacteriostatic Effect of CQ1 Metabolic products

Note that: "±" means mean ± standard deviation, and different lowercase letters after the numbers means that the Duncan new complex polar error method is annotated at a level of 0.05.

Example 6:

this example investigated the effect of Bacillus paralicheniformis CQ1 on tomato seed growth promotion.

CQ1 fermentation broth preparation: preparing LB liquid culture medium, picking a small amount of CQ1 single colony in the LB liquid culture medium by an inoculating loop, and culturing at 30 ℃ for 48h by a shaking table of 180 r/min. The bacterial content of the fermentation broth (bacterial content is more than or equal to 10 hundred million CFU/mL) is measured by adopting a flat plate dilution coating method.

The fermentation broth is diluted by 0, 10, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 times according to the gradient for standby. Treating tomato seeds at 55deg.C for 20min, sterilizing with 75% ethanol for 1min, cleaning with sterile water for 3 times, sterilizing with sodium hypochlorite for 30s, and cleaning with sterile water for 3 times; soaking the sterilized tomato seeds in fermentation liquid with different dilution gradients, and soaking for 3 hours by taking the seeds soaked in clear water as a reference; and then the seeds are orderly placed in a culture dish paved with a layer of moist sterile filter paper, 1mL of fermentation liquor diluted in different gradients is added into the culture dish, 1mL of clear water is added in contrast, the culture dish is sealed by a sealing film, the culture is carried out in a culture box at 30 ℃ for 48 hours, and the germination rates of the seeds are recorded for 24 hours and 48 hours. After the germination rate is recorded, the seeds are continuously cultivated, 1mL of fermentation liquor and clear water which are diluted in different gradients are supplemented for proper times, and after the seeds are cultivated for 7 days, the length of radicle of the seeds is measured by a ruler. CK group was a control group without fermentation broth treatment, 30 seeds per treatment.

As shown in the following Table 6, the highest germination rate of 24h seeds is CQ1800 times diluent and 1000 times diluent, the germination rate is 83.33%, and the germination rate is higher than that of the control 73.33% in both treatments; the maximum germination rate of the seeds is 800 times of diluent, the germination rate is 100 percent, the next is 700 times of diluent and 1000 times of diluent, the germination rate of the seeds is 90.00 percent, and the germination rate of the control is 76.67 percent. CQ1 fermentation broth has a tendency of high concentration inhibition and low concentration promotion on seed germination rate. Among them, the germination accelerating effect is preferably 800-fold dilution.

Tables 624h and 48h seed germination

As shown in Table 7 and FIG. 8 below, the seed radicle length was higher than the control (2.38 cm) at a dilution factor of 600 or higher, with the radicle length preferably being 800-fold (3.62 cm) followed by 900-fold (3.33 cm), 1000-fold (2.82 cm), 600-fold (2.80 cm) and 700-fold (2.46 cm) dilutions, respectively.

TABLE 7 seed radicle length

In summary, tomato seeds are taken as an example, the germination rate of the seeds and the growth promoting effect of the radicle length of the seeds are preferably CQ1800 times of diluent, and the dilution of CQ1 (the bacterial content is more than or equal to 10 hundred million CFU/mL) fermentation liquor is recommended to be 600-1000 times when the seeds are germinated by CQ 1.

Example 7:

this example investigated the growth promoting effect of Bacillus paralicheniformis CQ1 on tomato plants.

Preparing CQ1 (1X 10 ⁹ CFU/mL) fermentation liquor, and according to the seed-promoting effect, respectively diluting the fermentation liquor by 800 times and 1000 times to perform plant root irrigation treatment, and simultaneously diluting the fermentation liquor by 800 times (1X 10 ¹⁰ CFU/mL) of Bacillus belicus CY30 fermentation liquor and irrigating roots (CK) with clear water as a control. 3d after transplanting, 20mL of the transplanting liquid is irrigated every week for the first 4 weeks, other treatments are consistent, and irrigation is not performed after the transplanting liquid. Seed germination was measured for 2 months on plant data. The plant height and root length are measured by a ruler, the stem thickness is measured by a vernier caliper, and the fresh weight and dry weight of the overground part of the plant are measured by an electronic balance.

As shown in the following tables 8 and 9, the plant height, root length, stem thickness, fresh weight at the upper part of the root and dry weight of the plant are preferably CQ 1-800X, and the difference is remarkable compared with the control, and then CQ 1-1000X and CY 30-800X, and the difference is not remarkable. The plant data are combined, and the promoting effect is preferably CQ1-800×.

TABLE 8 plant height, root length and stem thickness of each treated plant

Note that: "±" means mean ± standard deviation, different lowercase letters after the numbers means that the Duncan new complex polar difference method differs significantly at a level of 0.05. The following is the same.

TABLE 9 fresh and Dry weight of the parts above ground

In conclusion, the bacillus paratyphenius CQ1 has broad-spectrum antibacterial property, has antibacterial effect on various plant pathogenic bacteria, and the secondary metabolite can inhibit the growth of the pathogenic bacteria; CQ1 salt tolerance concentration is less than or equal to 14%, and protease, ferrite and auxin IAA can be produced; has growth promoting effect on tomato plants after tomato seeds.

The foregoing examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention, and all designs that are the same or similar to the present invention are within the scope of the present invention.

Claims (10)

1. A bacillus vice CQ1, characterized in that: the Bacillus paratlicheniformis CQ1 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2023491.

2. The bacillus vice versa CQ1 according to claim 1, characterized in that: the 16S rDNA sequence of the Bacillus paratlicheniformis CQ1 is shown in SEQ ID NO. 1.

3. Use of the Bacillus paratlicheniformis CQ1 according to any of the claims 1-2 for secretion of auxins, proteases and siderophores.

4. Use of the bacillus paralicheniformis CQ1 according to any of the claims 1-2 for inhibiting pathogenic bacteria.

5. The use according to claim 4, wherein: the pathogenic bacteria include Alternaria solani, alternaria brassicae, alternaria zeae, phytophthora cucurbitae, sclerotinia lettuce, fusarium Lycopersici, curvularia lunata, botrytis cinerea, pitaya canker, and citrus anthracis.

6. Use of bacillus paralicheniformis CQ1 according to any of the claims 1-2 for promoting plant growth.

7. The use according to claim 6, wherein: the plant is tomato.

8. A bacillus paralicheniformis CQ1 metabolite, characterized in that the metabolite is prepared by the steps of: inoculating the bacillus paralicheniformis CQ1 according to any of claims 1-2 to an LB liquid culture medium for culture, centrifuging the cultured bacterial liquid, and filtering the bacterial body to obtain a sterile fermentation liquid, namely the metabolite.

9. Use of the metabolite according to claim 8 for inhibiting pathogenic bacteria.

10. The use according to claim 9, wherein: the pathogenic bacteria comprise Botrytis cinerea, fusarium solani, fusarium oxysporum, alternaria alternata, and strawberry anthracnose.