CN117871739A - Establishment of HPLC fingerprint of Tibetan medicine incarvillea crassifolia and antioxidation activity spectral efficiency analysis method - Google Patents
Establishment of HPLC fingerprint of Tibetan medicine incarvillea crassifolia and antioxidation activity spectral efficiency analysis method Download PDFInfo
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Abstract
The invention discloses a method for establishing HPLC fingerprint of Tibetan medicine incarvillea crassifolia and analyzing antioxidant activity spectral efficiency, belonging to the technical field of HPLC analysis of Tibetan medicine incarvillea crassifolia. The HPLC fingerprint of the incarvillea crassifolia methanol extract is established, the chromatographic peak separation effect is better, the peak shape is symmetrical, and the peak tailing phenomenon is reduced. 11 HPLC fingerprints of samples of Artemisia mi from different sources were established and 20 common peaks were identified. The method comprises the steps of carrying out primary comprehensive evaluation on a incarvillea crassipes sample through cluster analysis and principal component analysis, evaluating the antioxidant activity of the sample through various tests, establishing the effective spectrum relation of incarvillea crassipes through gray correlation analysis, and finding that the correlation degree of a chromatographic peak P10 and certain antioxidant activity indexes is high, so that the incarvillea crassipes sample is possibly a key component of antioxidant effect. The invention provides scientific basis for distinguishing incarvillea crassifolia and confusion products thereof, provides methodology reference for evaluating the antioxidant activity and quality control thereof, and has important value for promoting the quality research of medicinal materials.
Description
Technical Field
The invention belongs to the technical field of HPLC analysis of Tibetan medicine incarvillea crassifolia, and particularly relates to a method for establishing an HPLC fingerprint of Tibetan medicine incarvillea crassifolia and analyzing antioxidant activity spectral efficiency.
Background
The Tibetan medicine incarvillea, incarvillea and incarvillea is dry herb of the perennial herb incarvillea of Bignoniaceae, the Tibetan transliteration names are Eurotium, wu Quma, and the like, and the Tibetan transliteration names are mostly grown in the moist places of mountain lands and mainly distributed in the places such as Tibet rassa, nardostachys, japanese, eastern and Qinghai, gansu, yunnan, and the like. "Mi Sheng Porro Hua" in Jingzhu Bencao (Jingzhu herbal medicine) for treating ear diseases, purgation and bloating, yimai Liping and treating ear diseases. The incarvillea mi has warm nature, bitter and sweet taste, has the effects of regulating menstruation and activating blood circulation, dispelling wind-damp, diminishing inflammation and benefiting ears, and tonifying pulse, and is commonly used for treating irregular menstruation, rheumatalgia, otitis media, hypertension and other symptoms. The Artemisia mi contains chemical components such as phenethyl alcohol glycoside, flavonoid, terpenoid, alkaloid and the like. Modern pharmacological researches have shown that the incarvillea crassifolia has the effects of resisting inflammation, protecting liver, resisting cancer, resisting oxidation and the like.
The incarvillea crassifolia is a recorded variety of medicine standard (Tibetan medicine) of the Ministry of health of the people's republic of China (1995 edition), the medicine name is incarvillea crassifolia, and the incarvillea crassifolia cannot effectively identify the authenticity and control the quality of the medicine only through character description and processing methods. In addition, because of different medication habits in various places and most of Tibetan medicines are locally obtained or replaced by plants of the same genus, various medicinal materials named incarvillea but different basic sources circulate in the market. The "jiao hao" group source as recorded in the "Tibetan medicine Standard in Gansu province" is "the dry root of the plant Artemisia annua of Bignoniaceae". The "jiao hao" group source recorded in chinese materia medica (Tibetan medicine roll) is "flowers, seeds and roots of the tabacco plant Misheng Porroa flower".
At present, less researches are carried out on incarvillea crassipes, the existing researches are mainly focused on the aspects of chemical component separation identification and activity evaluation, the researches on HPLC finger print of incarvillea crassipes are not seen, and the incarvillea crassipes cannot be clearly distinguished from other confusing products; the relation of the spectrum effect of the antioxidant effect is still to be further studied.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the invention establishes the HPLC fingerprint of the incarvillea crassifolia and compares the HPLC fingerprint with the confusing products on the market; combining principal component analysis and cluster analysis to study the quality differences of the incarvillea crassifolia medicinal materials in different producing areas; evaluating the oxidation resistance of 11 batches of incarvillea crassifolia samples, and performing correlation analysis on the 11 batches of incarvillea crassifolia samples and the fingerprint spectrum thereof by adopting gray correlation analysis so as to dig out characteristic peaks related to oxidation resistance of incarvillea crassifolia; provides reference for enriching the medicine identification and quality evaluation methods and effectively developing and utilizing resources.
In order to achieve the above purpose, the following technical scheme is adopted: the invention provides a method for establishing HPLC fingerprint of Tibetan medicine incarvillea crassifolia and analyzing antioxidant activity spectrum effect, wherein the establishment of the fingerprint comprises the following steps:
s1, preparing a sample solution: accurately weighing 0.5g of incarvillea crassipes powder, placing in a conical flask with a plug, adding 25mL of methanol, weighing, performing ultrasonic treatment, cooling, supplementing weight loss with methanol, filtering with a 0.45 μm microporous filter membrane, and collecting filtrate to obtain sample solution;
s2, precision investigation: injecting the sample solution into a high performance liquid chromatograph, continuously injecting the sample for 6 times, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s3, repeatability investigation: taking the same batch of incarvillea crassifolia powder, preparing 6 parts of sample solution in parallel according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s4, stability investigation: taking incarvillea mi powder, preparing a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement at 0, 2, 4, 8, 12 and 24 hours, recording the chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s5, establishing a fingerprint spectrum: preparing 11 batches of incarvillea crassifolia samples into a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording chromatographic data of each batch of samples, introducing the data in a cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking an IC-1 spectrum as a reference spectrum, generating an HPLC fingerprint and a reference spectrum of 11 batches of incarvillea crassifolia methanol extract, calibrating the common peaks and performing similarity analysis;
s6, identification of a common peak and comparison of a confusing product map: detecting 5 batches of confusing products by the same method, recording chromatographic data of each batch of samples, introducing the data in the cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking a JH-1 spectrum as a reference spectrum, generating HPLC fingerprint and a control spectrum of 5 batches of confusing products methanol extract, and comparing with the control spectrum of the incarvillea crassifolia.
Further, the chromatographic detection conditions are:
using Zhongpu Science RD-C 18 A chromatographic column, 250mm×4.6mm,5 μm; the mobile phase is methanol (A) -0.1% phosphoric acid aqueous solution (B), and the flow rate is 1.0mL/min; the detection wavelength is 254nm, the column temperature is 30 ℃, the sample injection amount is 10 mu L, and gradient elution conditions are adopted as follows:
0～5min，10～25％A；
5～23min，25％A；
23～25min，25～40％A；
25～40min，40％A；
40～50min，40～60％A；
50～65min，60～90％A；
65～70min，90～10％A；
70～75min，10％A。
further, the ultrasonic treatment conditions in the preparation of the sample solution are as follows: the power is 400W, the frequency is 40kHz, and the time is 30min.
Further, the analysis method comprises the following steps:
S1、Fe 3+ reduction ability measurement: taking 1mL of a methanol solution of incarvillea crassifolia with the mass concentration of 10mg/mL, and respectively adding 0.2mol/L phosphate buffer solution with the pH of=6.6 and 0.03mol/L K 3 Fe(CN) 6 2mL of each solution is evenly mixed, placed at 50 ℃ for reaction for 20min, and then added with 0.6mol/L CCl 3 0.2mL of COOH solution, mixing, centrifuging at 1000r/min for 10min, collecting supernatant 40 μl, ultrapure water 110 μl and FeCl 0.006mol/L 3 100 mu L of the solution is placed in the same well of a 96-well plate together, evenly mixed, methanol is used as a blank control, the absorbance of the solution at 700nm is detected, each group of samples is repeatedly inspected for 3 times, and Fe 3+ Reducing ability (a) =a 1 －A 0 Wherein A is 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group; the larger the A value is, the stronger the reducing capability of the sample is indicated;
S2、DPPH · clean-up ability assay: taking 100 mu L of incarvillea crassifolia methanol solution with the mass concentration of 5mg/mL, and 0.1mmol/L DPPH · Mixing 3.90mL of the solution, performing light-shielding treatment, shaking at room temperature for 30min, taking 200 μl of the mixed solution, placing into a 96-well plate, measuring absorbance at 517nm, repeatedly detecting each group of samples for 3 times by taking methanol as a blank control, and performing DPPH cleaning ability= (1-A) 1 /A 0 ) X 100%, where A 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group;
S3、ABTS ·+ clean-up ability assay: taking a proper amount of 7mmol/L ABTS solution and 2.45mmol/L K 2 S 2 O 8 Mixing the solutions according to the volume ratio of 1:1, and reacting for 14h under the condition of dark room temperature to prepare the ABTS ·+ Stock solution, diluting the stock solution with 95% ethanol until the absorbance is 0.7+ -0.02 (734 nm), mixing 0.1mL of vitamin E (Trolox) solution with mass concentration of 50-450 μg/mL with 3.9mL of substrate solution, reacting at room temperature for 6min, collecting 200 μl of the mixed solution, placing into 96-well plate, detecting absorbance (734 nm), and measuring absorbance with mass concentration as abscissaMaking regression curve with the degree as ordinate to obtain linear equation, taking 0.1mL of Artemisia mi methanol solution with mass concentration of 2mg/mL to replace Trolox solution for reaction, detecting, substituting the obtained result into the linear equation, and substituting ABTS ·+ The scavenging capacity is expressed as Trolox antioxidant capacity equivalent TEAC (mg/g);
s4, FRAP measurement: taking a proper amount of 0.3mol/L CH 3 COONa buffer solution, TRTZ solution (40 mmol/L HCl solution as solvent) and FeCl solution (20 mmol/L) 3 Mixing according to the volume ratio of 10:1:1 to generate FRAP working solution, preserving heat in a water bath at 37 ℃, and precisely transferring FeSO with different concentrations (1-10 mmol/L) for use after preparation 4 ·7H 2 Adding 3mL of FRAP working solution into 20 μL of O solution, uniformly mixing, reacting at 37 ℃ for 40min, taking 200 μL of mixed solution, placing into a 96-well plate, detecting absorbance (593 nm), preparing a regression curve by taking concentration as an abscissa and absorbance as an ordinate, obtaining a linear equation, taking 20 μL of incarvillea crassifolia methanol solution with mass concentration of 10mg/mL to replace FeSO 4 ·7H 2 O solution is reacted and absorbance is detected, the obtained result is substituted into a linear equation and the FRAP value of a sample is calculated, and the total antioxidant capacity is calculated by FARP (FeSO 4 mmol/g).
Further, gray correlation analysis is carried out by taking the antioxidant activity of different batches of incarvillea crassifolia samples as a parent sequence and the obtained 20 common peak-to-peak areas as subsequences to obtain incarvillea crassifolia Fe 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The scavenging ability and the association degree of FRAP and the shared peak are preliminarily determined by the grade of the association degree, and the contribution of chemical components represented by the shared peak to the antioxidant activity is determined.
Further, the ABTS ·+ The linear equation for the clean-up capacity measurement was y= 0.2102X-1.78, r=0.9982.
Further, the FRAP measurement yields a linear equation of y=0.0747x+0.0573, r= 0.9978.
The beneficial effects of the invention are as follows:
(1) According to the invention, an HPLC fingerprint of a methanol extract of incarvillea crassipes is established, a wavelength scanning result in a range of 190-400 nm shows that the number of chromatographic peaks of a sample solution at 254nm is the largest, the peak height is higher, so 254nm is selected as a detection wavelength, a mobile phase optimization result shows that most chromatographic peaks can be separated by a methanol-0.1% phosphoric acid aqueous solution gradient elution mode, the peak tailing phenomenon can be effectively improved by adding 0.1% phosphoric acid into a water phase, the peak types are more symmetrical, 11 batches of incarvillea crassipes HPLC fingerprints of different batches are established under the condition, 20 common peaks are matched, and compared with reference substances such as apigenin, luteolin and the like, related chromatographic peaks are not identified, and P6, P10 and P11 chromatographic peak areas in the established HPLC fingerprint are large and are main chemical components;
(2) The invention preliminarily classifies and comprehensively evaluates the incarvillea crassipes sample through cluster analysis and principal component analysis, the used sample comprises multiple variable factors such as different growth periods (flowering period and fruit period), different parts (whole grass and land parts), different sources (self-picking, hospital and market purchasing, different production places) and the like, the material diversity is sufficient, 11 batches of samples are gathered into 3 types, the front part, the middle part and the rear part are respectively listed in the principal component analysis comprehensive ranking, 2 analysis results are nearly consistent, wherein the sample (IC-10, IC-8) in the III type is comprehensively ranked in front, the peak area of chromatographic peaks P6, P10 and P11 is larger, but whether the sample is an efficacy component is not clear, the research of the traditional Chinese medicine spectrum effect relation combines the fingerprint spectrum with the efficacy research, and can more objectively and comprehensively reflect the internal quality and the efficacy material basis of the traditional Chinese medicine, and the invention determines the Fe of the solution to be tested 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The clearing capacity and FRAP are used for detecting the antioxidation capacity of the sample, and further the spectral efficiency relation of the incarvillea crassifolia is established through gray correlation analysis, so that the P10 chromatographic peak and the ABTS are found ·+ The correlation degree between the scavenging capacity and FRAP is high, which shows that the P10 chromatographic peak is a characteristic peak of the anti-oxidation effect (the 2 indexes);
(3) According to the method, the HPLC fingerprint spectrum of the incarvillea crassipes is established, the spectrum of the incarvillea crassipes is obviously different from the spectrum of the confusing product, the established method can be used for distinguishing incarvillea crassipes from confusing products of incarvillea crassipes, the antioxidation activity of samples from different sources is evaluated, the characteristic peak of the antioxidation activity is analyzed by establishing a spectrum efficiency relationship, and a method reference is provided for the identification and quality evaluation of incarvillea crassipes medicinal materials.
Drawings
FIG. 1 is an HPLC fingerprint overlay of 11 samples of Artemisia crassifolia;
FIG. 2 is a superimposed HPLC fingerprint of 5 batches of incarvillea confusing;
FIG. 3 is a control fingerprint of Artemisia mi (A) and its confusion (B);
FIG. 4 is a principal component analysis scattergram (B) of 11 batches of incarvillea crassifolia cluster heat map (A)
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art based on the embodiments of the present invention without making any inventive effort are within the scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the present invention. The preferred methods and materials described herein are illustrative only and should not be construed as limiting the scope of the present application.
The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are commercially available unless otherwise specified.
The instrument used in the invention comprises:
BS224S electronic balance (certolite, germany); LPCD-E3000 blast drying oven (Shanghai dragon jumping instruments Co., ltd.); e2695-2998 high performance liquid chromatograph (PAD detector, wattsu technologies, usa); UPR-II-20L pure water instrument (Sichuan Upoaching technology Co., ltd.); VGT-2013QT ultrasonic cleaner (250W power, 40kHz frequency, guangdong ultra-sound Co., ltd.).
The reagent used in the invention comprises the following components:
methanol (chromatographic purity, TEDIA, usa); phosphoric acid (chromatographic purity, TEDIA, usa); the other reagents are all analytically pure, and the water is ultrapure water.
The medicinal materials used in the invention are as follows:
11 batches of incarvillea crassipes samples were collected, all samples were identified by the institute of food and drug inspection, tibetan autonomous region, daphne, researchers, as dry whole grass or aerial parts of the plant incarvillea crassipes of the family Bignoniaceae, and other 5 batches of incarvillea crassipes samples were confounded, and specific information is shown in table 1.
TABLE 111 batch sample information
Example 1
Establishment of HPLC fingerprint
Chromatographic conditions: zhongpu Science RD-C 18 Chromatographic column (250 mm. Times.4.6 mm,5 μm); the mobile phase is methanol (A) -0.1% phosphoric acid aqueous solution (B), and gradient elution is carried out: 0-5 min, 10-25% A; 5-23 min,25% A; 23-25 min, 25-40% A; 25-40 min,40% A; 40-50 min, 40-60% A; 50-65 min, 60-90% A; 65-70 min, 90-10% A; 70-75 min,10% A; the flow rate is 1.0mL/min; the detection wavelength is 254nm, the column temperature is 30 ℃, and the sample injection amount is 10 mu L.
Preparation of test solution: accurately weighing 0.5g of incarvillea crassipes powder, placing in a conical flask with a plug, adding 25mL of methanol, weighing, performing ultrasonic treatment, cooling, supplementing weight loss with methanol, filtering with a 0.45 μm microporous filter membrane, and collecting filtrate to obtain sample solution;
precision investigation: injecting the sample solution into a high performance liquid chromatograph, continuously injecting the sample for 6 times, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
repeatability investigation: taking the same batch of incarvillea crassifolia powder, preparing 6 parts of sample solution in parallel according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
stability investigation: taking incarvillea mi powder, preparing a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic determination at 0, 2, 4, 8, 12 and 24 hours, recording the chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating the RSD value.
The inspection results of precision, repeatability and stability show that the retention time of each peak and the RSD of the peak area are less than 2%, and the analysis requirements are met.
Establishing a fingerprint spectrum: preparing 11 batches of incarvillea crassifolia samples into a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording chromatographic data of each batch of samples, introducing the data in a cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking an IC-1 spectrum as a reference spectrum, generating an HPLC fingerprint and a reference spectrum of 11 batches of incarvillea crassifolia methanol extract, calibrating the common peaks and performing similarity analysis;
identification of common peaks and comparison of confounding product patterns: detecting 5 batches of confusing products by the same method, recording chromatographic data of each batch of samples, introducing the data in the cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking a JH-1 spectrum as a reference spectrum, generating HPLC fingerprint and a control spectrum of 5 batches of confusing products methanol extract, and comparing with the control spectrum of the incarvillea crassifolia.
HPLC finger print of Artemisia crassipes (HPLC) finger print superposition and comparison of methanol extract of Artemisia crassipes (HPLC) are shown in figures 1 and 3A, respectively, and 20 common peaks are obtained. The similarity between 11 batches of samples and the control pattern was 0.978, 0.982, 0.939, 0.663, 0.733, 0.620, 0.810, 0.962, 0.833, 0.921, and 0.881, respectively, wherein the similarity between IC-4, IC-5, and IC-6 was low, indicating that the difference between these 3 batches and the other batches was large.
The fingerprint spectrum superposition diagram and the comparison diagram of the methanol extract of 5 batches of incarvillea crassifolia confusing products are respectively shown in fig. 2 and 3B, and 22 common peaks are matched. As can be seen from fig. 3, there was a significant difference between the control pattern of the confounding product and the control pattern of the pachytene, and there were differences in the peak pattern, the number of peaks, and the retention time. The fingerprint method can distinguish the incarvillea crassifolia from the confusion products.
Example 2
Cluster analysis
And (3) taking 20 common peak areas of 11 batches of incarvillea crassifolia as original data, importing the original data into a scientific drawing cloud platform (https:// closed. Metal. Cn), performing Z-score standardization processing on the original data, and drawing a cluster heat map.
The result of the cluster analysis can reflect the proximity degree between samples in different batches, and the result of the cluster analysis heat map is shown in fig. 4 (a). As can be seen from the figure, 11 batches of Artemisia crassifolia can be roughly classified into 3 types, wherein samples (IC-5, IC-7, IC-11) from Tibetan Lin Zhi Hospital are collected into type I, and 3 batches are all whole plants (fruit period); samples from Tibetan Jiali county (IC-1, IC-2, IC-3), yala Xiangshan (IC-4) and Qinghai province (IC-9) were collected as class II, with 5 batches all being whole grass, 2 batches being flowering and 3 batches being fruit-bearing; 3 samples from Tibetan Natqu City (IC-6), chang City (IC-8) and mountain nan City (IC-10) were aggregated into class III, which were fruit phase samples, with 1 as the aerial part and 2 as the whole herb. It can be seen that the characteristic chromatographic peak area in incarvillea crassifolia is multiply affected by the place of production, growth phase and harvest site.
Example 3
Principal component analysis
Taking the peak areas of 20 common peaks in 11 batches of incarvillea fingerprint as variables, importing SPSS26.0 software for main component analysis, extracting 3 main components by taking characteristic values and accumulated contribution rate as judgment basis, and according to the following stepsAnd respectively calculating scores PCA1, PCA2 and PCA3 of the main components 1, 2 and 3, wherein alpha is a factor score, lambda is a characteristic value, PCA is a main component score, and then, calculating the comprehensive score F of 11 batches of samples according to F=0.501PCA1+0.279 PCA2+0.220PCA3, wherein the higher the F value, the higher the ranking, the higher the content of the common components in the samples.
Extracting 3 principal components PC according to the dimension reduction result 1 、PC 2 、PC 3 The variance accumulated contribution value reaches 74.015%, has strong representativeness, and can represent common peak information of the incarvillea crassifolia. The composite scores of the different samples were ranked as IC-10 > IC-8 > IC-9 > IC-4 > IC-1=IC-3 > IC-2 > IC-6 > IC-11 > IC-7 > IC-5. The samples from the Tibetan southwest city and the Chang city have high comprehensive scores, and the common peaks P10, P15, P3, P6, P8 and P11 of the 2 batches of samples are found to have high peak area heat response values by combining with a cluster analysis heat map, so that the common peaks are the main factors contributing to the front comprehensive scores. The composite scores of IC-9, IC-4, IC-1, IC-3 and IC-2 are in the middle and are consistent with the clustering results. After the comprehensive scores of the IC-5, the IC-7 and the IC-11 are ranked, the samples are also clustered into 1 type in the cluster analysis, and the samples are of the same source, and the common peak areas of the peaks P1, P10, P13, P15, P3, P6, P12, P7, P8 and P5 of the 3 batches of samples are found to have lower heat response values by combining with the cluster analysis heat map. With PC 1 、PC 2 、PC 3 As a coordinate value, a scattergram is drawn in FIG. 4 (B), from which it is known that the distributions of IC-5, IC-7, IC-11 and IC-6 are also at different extreme edges.
Example 4
In vitro antioxidant Activity assessment
Fe 3+ Reduction ability measurement: taking 1mL of a methanol solution of incarvillea crassifolia with the mass concentration of 10mg/mL, and respectively adding 0.2mol/L phosphate buffer solution with the pH of=6.6 and 0.03mol/L K 3 Fe(CN) 6 2mL of each solution is evenly mixed, placed at 50 ℃ for reaction for 20min, and then added with 0.6mol/L CCl 3 0.2mL of COOH solution, mixing, centrifuging at 1000r/min for 10min, collecting supernatant 40 μl, ultrapure water 110 μl and FeCl 0.006mol/L 3 100 mu L of the solution is placed in the same well of a 96-well plate together, evenly mixed, methanol is used as a blank control, and the mixture is inspectedThe absorbance at 700nm was measured and each set of samples was repeatedly examined 3 times, fe 3+ Reducing ability (a) =a 1 －A 0 Wherein A is 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group; the larger the A value is, the stronger the reducing capability of the sample is indicated;
DPPH · clean-up ability assay: taking 100 mu L of incarvillea crassifolia methanol solution with the mass concentration of 5mg/mL, and 0.1mmol/L DPPH · Mixing 3.90mL of the solution, performing light-shielding treatment, shaking at room temperature for 30min, taking 200 μl of the mixed solution, placing into a 96-well plate, measuring absorbance at 517nm, repeatedly detecting each group of samples for 3 times by taking methanol as a blank control, and performing DPPH cleaning ability= (1-A) 1 /A 0 ) X 100%, where A 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group;
ABTS ·+ clean-up ability assay: taking a proper amount of 7mmol/L ABTS solution and 2.45mmol/L K 2 S 2 O 8 Mixing the solutions according to the volume ratio of 1:1, and reacting for 14h under the condition of dark room temperature to prepare the ABTS ·+ The stock solution is diluted to have absorbance of 0.7+/-0.02 (734 nm) by using 95% ethanol before use, 0.1mL of vitamin E (Trolox) solution with mass concentration of 50-450 mug/mL is respectively taken and mixed with 3.9mL of substrate solution, reaction is carried out for 6min at room temperature, 200 mug of mixed solution is taken and placed in a 96-well plate, absorbance (734 nm) is detected, a regression curve is produced by taking mass concentration as an abscissa and absorbance as an ordinate, and a linear equation Y= 0.2102X-1.78 and r=0.9982 is obtained. Taking 0.1mL of artemisia mi methanol solution with the mass concentration of 2mg/mL to replace Trolox solution for reaction and detection, substituting the obtained result into the linear equation, and substituting ABTS ·+ The scavenging capacity is expressed as Trolox antioxidant capacity equivalent TEAC (mg/g);
FRAP assay: taking a proper amount of 0.3mol/L CH 3 COONa buffer solution, TRTZ solution (40 mmol/L HCl solution as solvent) and FeCl solution (20 mmol/L) 3 Mixing according to the volume ratio of 10:1:1 to generate FRAP working solution, and preserving heat in a water bath at 37 ℃ for preparation. Precisely removing FeSO with different concentrations (1-10 mmol/L) 4 ·7H 2 O solution 20. Mu.L, FRAP was added3mL of working solution is uniformly mixed, the mixture is reacted for 40min at 37 ℃, 200 mu L of the mixed solution is placed in a 96-well plate, the absorbance (593 nm) of the mixed solution is detected, a regression curve is prepared by taking the concentration as an abscissa and the absorbance as an ordinate, a linear equation Y=0.0747X+0.0573 and r= 0.9978 is obtained, and 20 mu L of a solution of incarvillea crassifolia methanol with the mass concentration of 10mg/mL is taken to replace FeSO 4 ·7H 2 O solution is reacted and absorbance is detected, the obtained result is substituted into a linear equation and the FRAP value of a sample is calculated, and the total antioxidant capacity is calculated by FARP (FeSO 4 mmol/g).
Performing gray correlation analysis by taking the antioxidant activity of different batches of incarvillea crassifolia samples as a parent sequence and the obtained 20 common peak areas as subsequences to obtain incarvillea crassifolia Fe 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The scavenging ability and the association degree of FRAP and the shared peak are preliminarily determined by the grade of the association degree, and the contribution of chemical components represented by the shared peak to the antioxidant activity is determined.
Fe of 11 batches of incarvillea crassifolia samples 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The scavenging capacity and the FARP are shown in table 2. The results show Fe of IC-1, IC-2 and IC-3 3+ Reducing power, DPPH · Scavenging capability and ABTS ·+ The cleaning capacity is the strongest; the FARP of IC-10 is obviously superior to that of other groups * P < 0.05). The 4 antioxidant activity indexes of IC-5, IC-6, IC-7 and IC-11 are the weakest, and are consistent with the ranking result of the principal component analysis comprehensive score, which shows that 20 common peaks serving as principal component analysis variables are related to the antioxidant capacity of the principal component analysis variables to some extent. Notably, the FARP of IC-10 is the strongest, but DPPH · The cleaning ability is weaker; IC-1 has extremely strong Fe 3+ Reduction ability and ABTS ·+ The clearance, but the FARP was slightly insufficient, and the 2 batches were clustered into different categories, indicating that the contribution of the different common peak components to the 4 antioxidant activity indicators was different.
Table 2 antioxidant results of each efficacy index
Example 5
Gray correlation analysis of fingerprint spectrum and antioxidant activity
By Fe 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The clearing capacity and FRAP are used as reference sequences, and peak areas of 20 common peaks are used as comparison sequences to perform gray correlation analysis. The correlation between 20 HPLC common peaks and 4 antioxidant activity indexes of Artemisia mi was calculated, and the results are shown in Table 3. The magnitude of the degree of association may reflect the magnitude of the association between the chemical component represented by each common peak and the antioxidant activity, with the closer the degree of association to 1, the greater the influence of the component represented by the peak on the antioxidant activity, indicating a stronger association of the chemical component with the antioxidant ability.
TABLE 3 correlation of common peaks with antioxidant Activity
| Peak number | Fe 3+ Reducing ability | DPPH cleaning ability | ABTS ·+ Scavenging ability | FRAP |
| P1 | 0.6708 | 0.5988 | 0.6766 | 0.7243 |
| P2 | 0.6730 | 0.5999 | 0.6805 | 0.6861 |
| P3 | 0.5681 | 0.6471 | 0.6085 | 0.6878 |
| P4 | 0.6225 | 0.6291 | 0.6267 | 0.6359 |
| P5 | 0.6590 | 0.6113 | 0.6728 | 0.6859 |
| P6 | 0.5786 | 0.6169 | 0.6457 | 0.7385 |
| P7 | 0.6658 | 0.6514 | 0.6386 | 0.6349 |
| P8 | 0.6232 | 0.6759 | 0.6046 | 0.6955 |
| P9 | 0.6480 | 0.7021 | 0.4923 | 0.5326 |
| P10 | 0.6212 | 0.5277 | 0.8490 | 0.8512 |
| P11 | 0.6250 | 0.5854 | 0.6070 | 0.6091 |
| P12 | 0.6832 | 0.6375 | 0.7183 | 0.7474 |
| P13 | 0.6136 | 0.5814 | 0.7775 | 0.7393 |
| P14 | 0.5480 | 0.7027 | 0.5307 | 0.5563 |
| P15 | 0.5576 | 0.6964 | 0.6820 | 0.7280 |
| P16 | 0.6678 | 0.6518 | 0.5179 | 0.5921 |
| P17 | 0.6028 | 0.6843 | 0.5003 | 0.5522 |
| P18 | 0.6197 | 0.6312 | 0.5961 | 0.6045 |
| P19 | 0.7029 | 0.5738 | 0.5613 | 0.5647 |
| P20 | 0.6984 | 0.5569 | 0.6024 | 0.5970 |
As is clear from the table, fe 3+ The association degree of the reduction capability is 0.5480-0.7029, DPPH · The relevance of the scavenging ability is 0.5277-0.7027, and the relevance value of the scavenging ability and all the common peaks is less than 0.8, which shows that the substance basis of the incarvillea crassifolia for exerting the 2 antioxidant abilities does not have the prominent contribution of a certain common peak, and the result of the multi-component comprehensive effect is possible. ABTS ·+ The relevance of the clearing capacity is 0.4923-0.8490, the relevance of FRAP is 0.5522-0.8512, wherein the relevance of the peak number P10 and the peak number 2 is more than 0.8, indicating that the peak number P10 is opposite to ABTS ·+ The contribution of scavenging capacity and FRAP is greatest; the correlation between peak number 9 and peak number 2 was minimal.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The invention and its embodiments have been described above with no limitation, and the invention is illustrated in the figures of the accompanying drawings as one of its embodiments, without limitation in practice. In summary, those skilled in the art, having benefit of this disclosure, will appreciate that the invention can be practiced without the specific details disclosed herein.
Claims (7)
1. The establishment of the HPLC fingerprint of the Tibetan medicine incarvillea is characterized in that: the establishment of the fingerprint comprises the following steps:
s1, preparing a sample solution: accurately weighing 0.5g of incarvillea crassipes powder, placing in a conical flask with a plug, adding 25mL of methanol, weighing, performing ultrasonic treatment, cooling, supplementing weight loss with methanol, filtering with a 0.45 μm microporous filter membrane, and collecting filtrate to obtain sample solution;
s2, precision investigation: injecting the sample solution into a high performance liquid chromatograph, continuously injecting the sample for 6 times, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s3, repeatability investigation: taking the same batch of incarvillea crassifolia powder, preparing 6 parts of sample solution in parallel according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording a chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s4, stability investigation: taking incarvillea mi powder, preparing a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement at 0, 2, 4, 8, 12 and 24 hours, recording the chromatogram, taking a No. 6 common peak as a reference peak, calculating the relative retention time and the relative peak area of the common peak, and calculating an RSD value;
s5, establishing a fingerprint spectrum: preparing 11 batches of incarvillea crassifolia samples into a sample solution according to the method in the step S1, injecting the sample solution into a high performance liquid chromatograph for chromatographic measurement, recording chromatographic data of each batch of samples, introducing the data in a cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking an IC-1 spectrum as a reference spectrum, generating an HPLC fingerprint and a reference spectrum of 11 batches of incarvillea crassifolia methanol extract, calibrating the common peaks and performing similarity analysis;
s6, identification of a common peak and comparison of a confusing product map: detecting 5 batches of confusing products by the same method, recording chromatographic data of each batch of samples, introducing the data in the cdf format into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, performing common peak matching by taking a JH-1 spectrum as a reference spectrum, generating HPLC fingerprint and a control spectrum of 5 batches of confusing products methanol extract, and comparing with the control spectrum of the incarvillea crassifolia.
2. The establishment of the Tibetan medicine incarvillea crassifolia HPLC fingerprint according to claim 1, which is characterized in that: the chromatographic detection conditions are as follows:
using Zhongpu Science RD-C 18 A chromatographic column, 250mm×4.6mm,5 μm; the mobile phase is methanol (A) -0.1% phosphoric acid aqueous solution (B), and the flow rate is 1.0mL/min; the detection wavelength is 254nm, the column temperature is 30 ℃, the sample injection amount is 10 mu L, and gradient elution conditions are adopted as follows:
0～5min，10～25％A；
5～23min，25％A；
23～25min，25～40％A；
25～40min，40％A；
40～50min，40～60％A；
50～65min，60～90％A；
65～70min，90～10％A；
70～75min，10％A。
3. the establishment of the Tibetan medicine incarvillea crassifolia HPLC fingerprint according to claim 2, which is characterized in that: the ultrasonic treatment conditions in the preparation of the sample solution are as follows: the power is 400W, the frequency is 40kHz, and the time is 30min.
4. A method for analyzing the antioxidant activity spectrum effect of Tibetan medicine incarvillea crassifolia according to claim 3, which is characterized in that: the analysis method comprises the following steps:
S1、Fe 3+ reduction ability measurement: taking 1mL of a methanol solution of incarvillea crassifolia with the mass concentration of 10mg/mL, and respectively adding 0.2mol/L phosphate buffer solution with the pH of=6.6 and 0.03mol/L K 3 Fe(CN) 6 2mL of each solution is evenly mixed, placed at 50 ℃ for reaction for 20min, and then added with 0.6mol/L CCl 3 0.2mL of COOH solution, mixing, centrifuging at 1000r/min for 10min, collecting supernatant 40 μl, ultrapure water 110 μl and FeCl 0.006mol/L 3 100 mu L of the solution is placed in the same well of a 96-well plate together, evenly mixed, methanol is used as a blank control, the absorbance of the solution at 700nm is detected, each group of samples is repeatedly inspected for 3 times, and Fe 3+ Reducing ability (a) =a 1 －A 0 Wherein A is 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group; the larger the A value is, the stronger the reducing capability of the sample is indicated;
S2、DPPH · clean-up ability assay: taking 100 mu L of incarvillea crassifolia methanol solution with the mass concentration of 5mg/mL, and 0.1mmol/L DPPH · Mixing 3.90mL of the solution, performing light-shielding treatment, shaking at room temperature for 30min, taking 200 μl of the mixed solution, placing into a 96-well plate, measuring absorbance at 517nm, repeatedly detecting each group of samples for 3 times by taking methanol as a blank control, and performing DPPH cleaning ability= (1-A) 1 /A 0 ) X 100%, where A 1 For the absorbance of the test sample group, A 0 Absorbance for the blank control group;
S3、ABTS ·+ clean-up ability assay: taking a proper amount of 7mmol/L ABTS solution and 2.45mmol/L K 2 S 2 O 8 Mixing the solutions according to the volume ratio of 1:1, and reacting for 14h under the condition of dark room temperature to prepare the ABTS ·+ Diluting the stock solution with 95% ethanol to absorbance of 0.7+ -0.02 (734 nm), respectively mixing 0.1mL of vitamin E (Trolox) solution with mass concentration of 50-450 μg/mL with 3.9mL of substrate solution, reacting at room temperature for 6min, placing 200 μl of the mixed solution in a 96-well plate, detecting absorbance (734 nm), preparing regression curve with mass concentration as horizontal coordinate and absorbance as vertical coordinate to obtain linear equation, taking 0.1mL of Artemisia crassifolia methanol solution with mass concentration of 2mg/mL to replace Trolox solution for reaction and detection, substituting the obtained result into the linear equation, and obtaining ABTS ·+ The scavenging capacity is expressed as Trolox antioxidant capacity equivalent TEAC (mg/g);
s4, FRAP measurement: taking a proper amount of 0.3mol/L CH 3 COONa buffer solution, TRTZ solution (40 mmol/L HCl solution as solvent) and FeCl solution (20 mmol/L) 3 Mixing according to the volume ratio of 10:1:1 to generate FRAP working solution, preserving heat in a water bath at 37 ℃, and precisely transferring FeSO with different concentrations (1-10 mmol/L) for use after preparation 4 ·7H 2 Adding 3mL of FRAP working solution into 20 μL of O solution, uniformly mixing, reacting at 37 ℃ for 40min, taking 200 μL of mixed solution, placing into a 96-well plate, detecting absorbance (593 nm), preparing a regression curve by taking concentration as an abscissa and absorbance as an ordinate, obtaining a linear equation, taking 20 μL of incarvillea crassifolia methanol solution with mass concentration of 10mg/mL to replace FeSO 4 ·7H 2 O solution reacts and detects absorbanceDegree, substituting the obtained result into a linear equation and calculating the FRAP value of the sample, and using FARP (FeSO 4 mmol/g).
5. The method for analyzing the antioxidant activity spectrum effect of Tibetan medicine incarvillea crassifolia according to claim 4, which is characterized in that: performing gray correlation analysis by taking the antioxidant activity of different batches of incarvillea crassifolia samples as a parent sequence and the obtained 20 common peak areas as subsequences to obtain incarvillea crassifolia Fe 3+ Reducing power, DPPH · Capacity of cleaning, ABTS ·+ The scavenging ability and the association degree of FRAP and the shared peak are preliminarily determined by the grade of the association degree, and the contribution of chemical components represented by the shared peak to the antioxidant activity is determined.
6. The method for analyzing the antioxidant activity spectrum effect of Tibetan medicine incarvillea crassifolia according to claim 5, which is characterized in that: the ABTS ·+ The linear equation for the clean-up capacity measurement was y= 0.2102X-1.78, r=0.9982.
7. The method for analyzing the antioxidant activity spectrum effect of Tibetan medicine incarvillea crassifolia according to claim 6, which is characterized in that: the linear equation obtained by the FRAP assay was y=0.0747x+0.0573, r= 0.9978.
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