CN117860926A - Disinfection cabinet, disinfection method and verification method of disinfection capacity - Google Patents

Disinfection cabinet, disinfection method and verification method of disinfection capacity Download PDF

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Publication number
CN117860926A
CN117860926A CN202311747058.8A CN202311747058A CN117860926A CN 117860926 A CN117860926 A CN 117860926A CN 202311747058 A CN202311747058 A CN 202311747058A CN 117860926 A CN117860926 A CN 117860926A
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China
Prior art keywords
disinfection
cabinet
cabinet body
sampling
result
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Pending
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CN202311747058.8A
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Chinese (zh)
Inventor
刘佳宇
曾南方
胡志坚
程陈凯
宁茂
高杰
胡慧
周俊亮
戴浩然
李晏
吴文斯
刘芬芬
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Giant Star Agriculture And Animal Husbandry Co ltd
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Giant Star Agriculture And Animal Husbandry Co ltd
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Priority to CN202311747058.8A priority Critical patent/CN117860926A/en
Publication of CN117860926A publication Critical patent/CN117860926A/en
Pending legal-status Critical Current

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Abstract

The invention provides a disinfection cabinet, a disinfection method and a verification method of disinfection capacity, and aims to solve the technical problem that pathogens on part of electronic equipment cannot be inactivated. The sterilizer comprises: the cabinet body is internally provided with a disinfection zone, and one side of the cabinet body is provided with a cabinet door; the glass partition plate is transversely arranged in the cabinet body, and spaces are reserved between the glass partition plate and the top surface and the bottom surface of the inner side of the cabinet body; and the disinfection lamp is arranged in the cabinet body. The disinfection lamp is arranged in the cabinet body, and the object to be disinfected is placed on the glass partition board, and the light rays of the disinfection lamp penetrate through the glass partition board to disinfect the bottom surface of the object to be disinfected. The disinfection method comprises the following steps: removing organic matters on the surface of the object to be disinfected; placing the object to be disinfected on a glass partition board in a disinfection cabinet, and starting a disinfection lamp for irradiation. The surface of the article to be disinfected is treated by removing organic matters. Then the material is placed on a glass partition board in a disinfection cabinet to carry out omnibearing disinfection treatment on the articles to be disinfected.

Description

Disinfection cabinet, disinfection method and verification method of disinfection capacity
Technical Field
The invention relates to the technical field of sterilization of animal husbandry, in particular to a sterilizing cabinet, a sterilizing method and a sterilizing capacity verification method.
Background
In livestock breeding, staff enters a breeding area to be disinfected, electronic equipment carried by staff is disinfected, such as a mobile phone, a watch and the like, the electronic equipment cannot be disinfected through disinfectant water, the traditional mode is to disinfect through ultraviolet light irradiation, and in actual operation, pathogens on part of the electronic equipment cannot be inactivated by an ultraviolet lamp.
Disclosure of Invention
Aiming at the technical problem that pathogens on part of electronic equipment cannot be inactivated in the prior art, the invention provides a test method for testing disinfection effect, which has the advantage that the reason that pathogens on part of electronic equipment cannot be inactivated can be explored. The invention also provides a disinfection cabinet and a disinfection method, which have the advantage of thoroughly inactivating pathogens on electronic equipment.
The technical scheme of the invention is as follows:
a sterilizer, comprising:
the cabinet body is internally provided with a disinfection zone, and one side of the cabinet body is provided with a cabinet door;
the glass partition plate is transversely arranged in the cabinet body, and spaces are reserved between the glass partition plate and the top surface and the bottom surface of the inner side of the cabinet body;
the disinfection lamp is arranged in the cabinet body;
wherein the light transmission wavelength range of the glass separator is 220nm-2500nm.
Optionally, the glass separator has a transmittance of greater than 90%;
at least one disinfection lamp is arranged on each inner wall surface of the cabinet body, and the luminous sides of all the disinfection lamps are opposite to the glass partition plate.
A method of sterilizing a sterilizer, comprising:
removing organic matters on the surface of the object to be disinfected;
placing the object to be disinfected on a glass partition board in a disinfection cabinet, starting a disinfection lamp, and irradiating for 5-40 min.
Optionally, the irradiation time of the sterilizing lamp is 8min-10 min.
Alternatively, the surface of the article to be sterilized is wiped with a 75% strength wine wiper or potassium hydrogen persulfate compound and wiped at least twice.
Optionally, when the potassium hydrogen persulfate compound is adopted to wipe the surface of an article to be disinfected, the ratio of the potassium hydrogen persulfate compound powder to the water is 1:80-1:120.
A method for verifying the disinfection capacity of a disinfection cabinet, comprising:
s10, selecting a plurality of articles to be detected to be divided into n parts, and respectively marking the n parts as P1, P2, P3 and P4;
s20, smearing pathogens on the P1, and sampling after the P1 is irradiated by a sterilizing lamp to obtain a result U28;
s30, mixing pathogens and organic matters, smearing the mixture on P2, and directly sampling to obtain a comparison result U10; wiping P2 with alcohol and sampling to obtain a result U11; irradiating P2 under a sterilizing lamp, and sampling to obtain a result U12; sampling after the sterilizing lamp irradiates P3 to obtain a result U14;
s40, smearing pathogens and organic matters on the P4, wiping the P4 by using a potassium hydrogen persulfate compound, and sampling to obtain a comparison result U312; and then P4 is placed under a sterilizing lamp for irradiation and then sampling is carried out, and a result U38 is obtained.
Optionally, in S20, the pathogen is added into physiological saline and mixed uniformly;
in S30, the pathogen and the organic substance are mixed and diluted with physiological saline to form a thick liquid.
Optionally, in S30, at least two alcohol wipes are performed on P2;
in S40, P4 is wiped with the potassium hydrogen persulfate complex at least twice.
Alternatively, the pathogen and organics are allowed to air for at least 10 minutes after the pathogen is applied to P1 and after the pathogens and organics are applied to P2 and P4.
Compared with the prior art, the invention has the beneficial effects that:
the disinfection lamp is arranged in the cabinet body, and the object to be disinfected is placed on the glass partition board, and the light rays of the disinfection lamp penetrate through the glass partition board to disinfect the bottom surface of the object to be disinfected.
In addition, according to the verification method of the disinfection capability of the disinfection cabinet, the reason that the pathogen cannot be inactivated is known to be that the surface of the object to be disinfected is shielded by the organic matters, so that the disinfection light cannot penetrate, and the bottom surface of the object to be disinfected is not irradiated with the disinfection light.
Therefore, the disinfection method in the invention is to remove organic matters on the surface of the object to be disinfected before the object to be disinfected is placed in the disinfection cabinet. Then placing the object to be sterilized in the sterilizing cabinet disclosed by the invention, and positioning the object to be sterilized on the glass partition board to perform omnibearing sterilization treatment on the object to be sterilized.
By the invention, pathogens on the articles to be disinfected can be effectively inactivated.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a table of test data of step S30 of the present invention;
FIG. 2 is a table of test data of step S20 of the present invention;
FIG. 3 is a table of test data of step S40 of the present invention;
fig. 4 is a schematic perspective view of a sterilizing cabinet;
FIG. 5 is a table of data for a test set of glass separators.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
The following disclosure provides many different embodiments, or examples, for implementing different features of the invention. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1:
referring to fig. 4, a sterilizing cabinet comprises a cabinet body 1, a glass partition plate 2 and a plurality of sterilizing lamps 4, wherein the cabinet body 1 is of a square structure, the inside is a sterilizing zone of a hollow structure, a cabinet door 3 is arranged on the front side of the cabinet body 1, a plurality of sterilizing lamps 4 are arranged in the cabinet body 1, at least one sterilizing lamp 4 is arranged on each wall surface in the cabinet body 1, at the same time, at least one sterilizing lamp 4 is also arranged on the cabinet door 3, and the luminous surfaces of all the sterilizing lamps 4 can irradiate on the glass partition plate 2.
The glass partition plate 2 is arranged in the middle of the cabinet body 1, and in this embodiment, the glass partition plate 2 is selected to be a glass partition plate, and the light transmission wavelength range of the glass partition plate 2 is 220nm-2500nm, and the light transmission is more than 90%. In this embodiment, by providing a plurality of sterilizing lamps 4 on the outer periphery of the glass partition plate 2, respectively, each face of the article to be sterilized can be irradiated.
The ultraviolet light can penetrate the glass partition board 2 within the wavelength range of 185nm-400nm, and the peak value of the light wavelength of the sterilizing lamp 4 is 245nm, so that the glass partition board 2 can be used for passing the ultraviolet light with the wavelength of 220nm-400nm, and the peak value of the ultraviolet light can also pass.
When the glass separator 2 was selected, the following two sets of tests were performed:
a first group: and placing the object to be tested, on which pathogens are not smeared, in a sterilizing cabinet, and irradiating the object to be tested under ultraviolet light for 40min in batches for sampling, wherein the materials of the glass partition plates used in different batches of tests are quartz glass, 5mm toughened glass and 10mm toughened glass respectively.
Second group: the articles to be measured coated with pathogens and organic matters are divided into a plurality of groups, the articles to be measured are sampled after being irradiated for 40min in batches, and the glass partition plate materials used in each batch are quartz glass, 5mm toughened glass and 10mm toughened glass respectively during irradiation.
The data in fig. 5 were obtained according to the two above-described sets of experiments. According to the data in fig. 5, both the control group pathogen and bacteria were positive, and the control was established. Under the condition that the organic matters exist on the object to be detected, the pathogen inactivation effect of ultraviolet light passing through the quartz glass is superior to that of 10mm toughened glass and 5mm toughened glass. In the state of no organic matter on the object to be detected, the ultraviolet light passes through the quartz glass to inactivate pathogens, while the ultraviolet light passes through the 10mm toughened glass and the 5mm toughened glass to inactivate pathogens.
In addition, under the condition that organic matters exist on the object to be detected, the inactivation effect of ultraviolet light passing through 10mm toughened glass and 5mm toughened glass on bacteria is obviously weaker than the inactivation effect of ultraviolet light passing through quartz glass on bacteria.
Example 2:
a disinfection method of a disinfection cabinet mainly aims at disinfecting electronic equipment entering an animal pasture area. Comprising the following steps:
the first step, the organic matters on the surface of the electronic equipment are cleaned in a wiping mode, and the cleaning method mainly comprises various organic matters stuck on the electronic equipment in the working process of staff and small amounts of organic matters secreted by sweat glands of human bodies.
And secondly, placing the object to be disinfected in a disinfection cabinet, and irradiating for 5-40 min by using a disinfection lamp.
Preferably, in the second step, the irradiation time of the sterilizing lamp is 8min-10 min. And the surface of the article to be sterilized is wiped with a 75% strength wine wiper or potassium hydrogen persulfate compound and wiped at least twice. When the potassium hydrogen persulfate compound is used for wiping the surface of an article to be disinfected, the ratio of the potassium hydrogen persulfate compound powder to water is 1:80-1:120 (the ratio is usually selected to be 1:100).
Example 3:
a method for verifying the disinfection capacity of a disinfection cabinet comprises the following steps:
s10, selecting a plurality of to-be-detected articles to be divided into 4 parts, wherein the number of the to-be-detected articles in each part is equal, and marking the 4 to-be-detected articles as P1, P2, P3 and P4 respectively.
S20, smearing pathogens on the P1, placing the P1 on a glass partition plate in a cabinet body, irradiating for a certain time through a sterilizing lamp, taking out and sampling to obtain a result U28.
S30, mixing pathogens and organic matters, smearing the mixture on P2, directly sampling the P2, and obtaining a comparison result U10; then wiping P2 with alcohol and sampling to obtain a result U11; p2 after being wiped by alcohol is placed into a cabinet body, and is sampled after being irradiated for a certain time under a sterilizing lamp, so that a result U12 is obtained; directly placing the P3 into a cabinet body without any treatment, irradiating for the same time by using a sterilizing lamp, and then sampling to obtain a result U14.
S40, smearing pathogens and organic matters on the P4, wiping the P4 by using a potassium hydrogen persulfate compound, and sampling to obtain a comparison result U312; and then placing the P4 on a glass partition plate in the cabinet body, and sampling after a certain time of irradiation by a sterilizing lamp to obtain a result U38.
Specific:
in step S20, an organic-free test group is added in a mode of simulating pathogen (pseudorabies vaccine/escherichia coli), and the method comprises the following steps:
step S21, adding physiological saline into pathogens, uniformly mixing, smearing on P1, and sampling at P1 to obtain a result U20; the pathogen is mixed with the organic matter and diluted with physiological saline to form a thick liquid to form the control of step S20.
In step S22, the part P1 is placed under a sterilizing lamp to be irradiated for 20min, and then sampled, so as to obtain a result U28.
In step S23, another part P1 is placed under a sterilizing lamp to be irradiated for 40min, and then sampled, thereby obtaining a result U29.
Step S24, according to the mode of steps S12-S15, the following data are obtained: sampling result U21 of P1 coated with pathogens and organic matters by alcohol wiping twice; the P1 coated with pathogens and organic matters is wiped twice by alcohol and then irradiated for 20min under ultraviolet light, and then a result U22 is sampled; the P1 coated with pathogens and organic matters is wiped twice by alcohol and is irradiated for 40min under ultraviolet light, and then a result U23 is sampled; p1 coated with pathogens and organic matters is directly irradiated under ultraviolet light for 20min, and then a result U26 is sampled; the results U27 were sampled after the pathogen and organic coated P1 was directly irradiated under UV light for 40min.
S25, after the P1 coated with pathogens and organic matters is wiped by clear water, the sample is taken after 20 minutes of irradiation under ultraviolet light; the P1 coated with pathogens and organic matters is wiped by clean water and then irradiated for 20min under ultraviolet light, and then the result U25 is sampled.
In step S20, a pathogen test group without organic matters is added, and the pathogen positive control without organic matters is not established. From the data shown in fig. 2, it can be seen that the bacterial results were negative for all the experimental treatment groups. In the organic pathogen group, direct ultraviolet irradiation is performed for 20min without wiping and ultraviolet irradiation is performed for 40min after clear water wiping, and the detected part P1 is positive and suspected to be shielding of organic substances, so that pathogens caused by the fact that ultraviolet light cannot penetrate the organic substances are not inactivated.
Step S30 includes the steps of:
step S31, mixing the pathogen and the organic matters, and diluting with normal saline to form thick liquid;
and S32, coating the thick liquid on the P2, airing the P2 for 10min, and sampling the P2 to obtain a comparison result U10.
In step S33, the alcohol wipes P2 in S32 twice, and samples are taken, thereby obtaining a result U11.
And step S34, placing the P2 in the step S33 in a disinfection cabinet, and sampling after irradiation by a disinfection lamp to obtain a result U12.
And step S35, placing the P3 in a disinfection cabinet, and sampling after irradiation by using a disinfection lamp to obtain a result U14.
The step S34 includes: step S341, irradiating the part P2 sterilizing lamp passing through the step S33 for 20min, and then sampling to obtain a result U12; and S342, irradiating the other part of the P2 sterilizing lamp which passes through the step S33 for 40min, and sampling to obtain a result U13.
The step S35 includes: step S351, directly irradiating the part P3 by a sterilizing lamp for 20min, and sampling to obtain a result U14; step S351, another part P3 is irradiated by a sterilizing lamp for 40min, and the result U15 is obtained by sampling.
Referring to the test data of step S30 shown in fig. 1, it can be derived that: 1. the bacterial positive control was established, and the positive result was found in the test treatment group, so that the pathogen was judged positive. 2. The method comprises the steps of wiping alcohol treatment and irradiation by a disinfection lamp after wiping alcohol, sampling viruses, wherein the ct value of 48h is larger than the ct value of 0h, the bacterial result is negative, and the objects positioned below the electronic equipment in an experimental group which is not directly irradiated by alcohol wiping are positive, so that the effects of irradiation after wiping with alcohol and single alcohol wiping are shown.
In step S40, the simulated pathogen arrangement without and with the presence of organic matter is the same as that of step S30 and step S20, and in step S40, the arrangement ratio of the potassium hydrogen persulfate complex solution is 1:100.
Step S40 includes the steps of:
in step S41, the pathogen and the organic matter are mixed and diluted by normal saline to form thick liquid, the thick liquid is smeared on P4, a control group is arranged, and sampling is carried out to obtain a result U35. Referring to step S21 in step S20, physiological saline is added to the pathogen, mixed uniformly, smeared onto P4, sampled to obtain a control group, and the sampling result is U31.
Step S42, wiping the potassium hydrogen persulfate compound solution twice with P4 in the step S41, and sampling to obtain a result U312;
in step S43, the P4 after wiping the potassium hydrogen persulfate compound solution in step S42 is divided into three groups, and the obtained groups are irradiated under ultraviolet light for 5min, 10min and 20min, and the sampling results U36, U37 and U38 are recorded.
In step S44, the P4 without organic matter is placed under ultraviolet light to irradiate for 5min, 10min and 20min, and the sampling results U32, U33 and U34 are recorded respectively.
And S45, wiping the P4 smeared with pathogens and organic matters twice by alcohol, sampling and recording the result as U313, dividing the objects to be detected after wiping the alcohol into three groups, respectively placing the three groups under ultraviolet light for irradiation for 5min, 10min and 20min, and respectively recording the sampling results as U39, U310 and U311.
From the data shown in fig. 3, it is possible to obtain, in step S40, a further reduction in the irradiation time of the test group and wiping with a potassium hydrogen persulfate complex solution conventionally used in actual production. Both pathogens and bacteria were positive, and the control group was established. The bacteria test groups are negative, the ct value of the pathogen test groups is increased compared with 0h for 48h, and the result shows that organic matters are important influencing factors of ultraviolet sterilization, so that before ultraviolet light is irradiated, organic matter residues on the surface of the electronic social security are necessary to be removed.
In this example, a virus sample infects PK-15 cells, and a result of determination of Ct value is detected in pathogenic nucleic acid in cell supernatants at an initial stage and after 48-h infection. Bacterial samples were plated for blue and white differential culture.
In this example, the alcohol wiper used was alcohol cotton with a concentration of 75%. All are placed in a disinfection cabinet for ultraviolet irradiation.
In conclusion, after the organic matters are removed, pathogenic microorganisms on the surface of the object to be detected can be killed by ultraviolet irradiation for 5-40 min. The ultraviolet irradiation sterilization can greatly shorten the sterilization time of the to-be-tested object and reduce the damage of the to-be-tested object caused by fumigation sterilization. According to the test result and in combination with the actual use scene, the object to be tested is sterilized mostly when personnel enter the isolation center or the pig farm is bathed, the irradiation time is recommended to be 8-10 min, the loss of the ultraviolet lamp tube can be increased due to the long-time irradiation, and the replacement cost of the ultraviolet lamp tube is increased.
The foregoing examples merely illustrate specific embodiments of the invention, which are described in greater detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims (10)

1. A sterilizer, comprising:
the cabinet body is internally provided with a disinfection zone, and one side of the cabinet body is provided with a cabinet door;
the glass partition plate is transversely arranged in the cabinet body, and spaces are reserved between the glass partition plate and the top surface and the bottom surface of the inner side of the cabinet body;
the disinfection lamp is arranged in the cabinet body;
wherein the light transmission wavelength range of the glass separator is 220nm-2500nm.
2. A disinfection cabinet as claimed in claim 1, wherein,
the transmittance of the glass separator is greater than 90%;
at least one disinfection lamp is arranged on each inner wall surface of the cabinet body, and the luminous sides of all the disinfection lamps are opposite to the glass partition plate.
3. A method of sterilizing a sterilizer as claimed in any one of claims 1 or 2, comprising:
removing organic matters on the surface of the object to be disinfected;
placing the object to be disinfected on a glass partition board in a disinfection cabinet, starting a disinfection lamp, and irradiating for 5-40 min.
4. A disinfection method as claimed in claim 3, wherein,
the irradiation time of the sterilizing lamp is 8min-10 min.
5. A disinfection method as claimed in claim 3, wherein,
the surface of the article to be sterilized is wiped with a 75% strength wine wiper or potassium hydrogen persulfate compound and wiped at least twice.
6. A disinfection method as claimed in claim 5, wherein,
when the potassium hydrogen persulfate compound is adopted to wipe the surface of an article to be disinfected, the proportion of the potassium hydrogen persulfate compound powder to the water is 1:80-1:120.
7. A method of verifying the disinfection capability of a disinfection cabinet as defined in claim 1, comprising:
s10, selecting a plurality of articles to be detected to be divided into n parts, and respectively marking the n parts as P1, P2, P3 and P4;
s20, smearing pathogens on the P1, and sampling after the P1 is irradiated by a sterilizing lamp to obtain a result U28;
s30, mixing pathogens and organic matters, smearing the mixture on P2, and directly sampling to obtain a comparison result U10; wiping P2 with alcohol and sampling to obtain a result U11; irradiating P2 under a sterilizing lamp, and sampling to obtain a result U12; sampling after the sterilizing lamp irradiates P3 to obtain a result U14;
s40, smearing pathogens and organic matters on the P4, wiping the P4 by using a potassium hydrogen persulfate compound, and sampling to obtain a comparison result U312; and then P4 is placed under a sterilizing lamp for irradiation and then sampling is carried out, and a result U38 is obtained.
8. The method of authentication of claim 7,
in the step S20, the pathogen is added into physiological saline to be uniformly mixed;
in S30, the pathogen and the organic substance are mixed and diluted with physiological saline to form a thick liquid.
9. The method of authentication of claim 7,
in the step S30, at least two alcohol wipes are performed on the P2;
in S40, P4 is wiped with the potassium hydrogen persulfate complex at least twice.
10. The test method for disinfection effects according to claim 7, wherein,
and after the pathogen is smeared on the P1 and the pathogen and the organic matters are smeared on the P2 and the P4, the air drying is carried out for at least 10min.
CN202311747058.8A 2023-12-19 2023-12-19 Disinfection cabinet, disinfection method and verification method of disinfection capacity Pending CN117860926A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311747058.8A CN117860926A (en) 2023-12-19 2023-12-19 Disinfection cabinet, disinfection method and verification method of disinfection capacity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311747058.8A CN117860926A (en) 2023-12-19 2023-12-19 Disinfection cabinet, disinfection method and verification method of disinfection capacity

Publications (1)

Publication Number Publication Date
CN117860926A true CN117860926A (en) 2024-04-12

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Application Number Title Priority Date Filing Date
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