CN117805217A - Human blood essential trace element detection method based on scientific research application - Google Patents
Human blood essential trace element detection method based on scientific research application Download PDFInfo
- Publication number
- CN117805217A CN117805217A CN202311855213.8A CN202311855213A CN117805217A CN 117805217 A CN117805217 A CN 117805217A CN 202311855213 A CN202311855213 A CN 202311855213A CN 117805217 A CN117805217 A CN 117805217A
- Authority
- CN
- China
- Prior art keywords
- sample
- essential trace
- standard
- human blood
- scientific research
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 54
- 239000008280 blood Substances 0.000 title claims abstract description 54
- 239000011573 trace mineral Substances 0.000 title claims abstract description 41
- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 41
- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000011160 research Methods 0.000 title claims abstract description 24
- 239000012086 standard solution Substances 0.000 claims abstract description 35
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims abstract description 31
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000003085 diluting agent Substances 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 26
- 239000011651 chromium Substances 0.000 claims abstract description 17
- 239000011669 selenium Substances 0.000 claims abstract description 15
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052804 chromium Inorganic materials 0.000 claims abstract description 14
- 229910017052 cobalt Inorganic materials 0.000 claims abstract description 14
- 239000010941 cobalt Substances 0.000 claims abstract description 14
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052759 nickel Inorganic materials 0.000 claims abstract description 14
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 claims abstract description 14
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000010949 copper Substances 0.000 claims abstract description 13
- 229910052742 iron Inorganic materials 0.000 claims abstract description 13
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 13
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000011701 zinc Substances 0.000 claims abstract description 12
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 12
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims abstract description 11
- 229910052720 vanadium Inorganic materials 0.000 claims abstract description 11
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 10
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052802 copper Inorganic materials 0.000 claims abstract description 10
- 238000005259 measurement Methods 0.000 claims abstract description 10
- 229910052750 molybdenum Inorganic materials 0.000 claims abstract description 10
- 239000011733 molybdenum Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 238000007865 diluting Methods 0.000 claims abstract description 8
- WUUZKBJEUBFVMV-UHFFFAOYSA-N copper molybdenum Chemical compound [Cu].[Mo] WUUZKBJEUBFVMV-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000010257 thawing Methods 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 53
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 21
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000013504 Triton X-100 Substances 0.000 claims description 10
- 229920004890 Triton X-100 Polymers 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 239000012496 blank sample Substances 0.000 claims description 6
- 239000012490 blank solution Substances 0.000 claims description 6
- 238000003908 quality control method Methods 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 238000012544 monitoring process Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 230000035945 sensitivity Effects 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 238000010884 ion-beam technique Methods 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 230000005856 abnormality Effects 0.000 claims 2
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000011572 manganese Substances 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 239000012488 sample solution Substances 0.000 description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 3
- 238000009616 inductively coupled plasma Methods 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- JBQYATWDVHIOAR-UHFFFAOYSA-N tellanylidenegermanium Chemical compound [Te]=[Ge] JBQYATWDVHIOAR-UHFFFAOYSA-N 0.000 description 3
- 238000009210 therapy by ultrasound Methods 0.000 description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000463291 Elga Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 231100000317 environmental toxin Toxicity 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000010437 erythropoiesis Effects 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000010438 iron metabolism Effects 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000020802 micronutrient deficiency Nutrition 0.000 description 1
- 238000000120 microwave digestion Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical class [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a method for detecting essential trace elements of human blood based on scientific research application, which comprises the following steps: selecting standard solutions of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium; diluting standard solutions of vanadium, chromium, manganese, cobalt, nickel and copper molybdenum into 0.0,0.1, 0.5, 1.0, 2.5, 5.0, 10, 20, 50 and 75ng/mL through a diluent; diluting standard solutions of iron, zinc and selenium into 0.0,1, 5, 10, 25, 50, 100, 200, 500 and 750ng/mL by using a diluent; drawing a standard curve; placing the diluted standard solution in an instrument to generate a blood essential trace element detection and measurement formula; taking out the blood sample from the refrigerator at-80 ℃, thawing in the refrigerator at 4 ℃, and fully vibrating and uniformly mixing; after the blood sample is uniformly mixed, 200 microliters of the sample is sucked, 3.8 milliliters of diluent is added to be mixed to form a sample to be detected; leading the sample to be detected into an instrument, and detecting and calculating essential trace elements of human blood by using a blood essential trace element detection and calculation formula; and generating a result.
Description
Technical Field
The invention relates to the technical field of blood essential trace elements, in particular to a method for detecting human blood essential trace elements based on scientific research application.
Background
It is currently widely accepted that exposure to certain factors (such as nutrition, stress and environmental toxins) during critical windows of ontogenesis (sensitive stages of fetal development, infancy and childhood) can have profound effects on organ and tissue development; similarly, human and animal studies have well proven that the pregnancy period is a critical window during which external environmental exposure (e.g., metal exposure) adversely affects the long-term risk of endocrinological metabolism, cardiovascular, immune and neurological disorders in the offspring.
However, current research is still lacking in related mechanisms for specific time windows (e.g., childhood exposure at different stages), exposure concentrations, mixed exposure or exposure and genes, exposure and outcome.
World health organization (World Health Organization, WHO) estimated that more than 20 hundred million people were deficient in key vitamins and minerals, especially iron and zinc. These micronutrient deficiencies are also associated with increased morbidity and severity of infectious diseases such as diarrhea, measles, malaria and pneumonia, and mortality.
Too much or too little essential trace elements affect the balance of the elements in the human body and increase the risk of chronic diseases.
Wherein microelements essential for human blood comprise manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), selenium (Se), molybdenum (Mo), vanadium (V) and chromium (Cr);
iron (Fe) is an essential component in erythrocytes for transporting oxygen; zinc (Zn) contributes to the normal functioning of the immune system and cell division; copper (Cu) is involved in many biological reactions, including iron metabolism and energy production; cobalt (Co) is part of vitamin B12, necessary for erythropoiesis; manganese (Mn) exerts an antioxidant effect and participates in bone formation; molybdenum (Mo) plays a key role in the activity of certain enzymes; nickel (Ni) may contribute to iron absorption, participating in hormone, fat and membrane functions; vanadium (V) may contribute to cellular metabolism and inhibit cholesterol synthesis; chromium (Cr) helps to enhance the action of insulin, promoting the uptake and utilization of glucose by cells.
The existing Atomic Absorption Spectrometry (AAS) has high detection limit and low sensitivity, and can only measure one heavy metal at a time; the microwave digestion method has complicated steps, low efficiency and multiple steps, is easy to pollute the sample treatment process, and is not suitable for pretreatment operation of a large number of samples.
Currently, studies on heavy metal levels in the blood urine of children across the country are very lacking and no routine monitoring is performed, thus further screening for endogenous heavy metal exposure levels in children is required. The research result lays a foundation for further providing preventive measures and reducing the harm of environmental pollutants to early childhood development.
The foregoing description is provided for general background information and does not necessarily constitute prior art.
Disclosure of Invention
The invention aims to provide a human blood essential trace element detection method based on scientific research application, which evaluates the current situation of essential trace element exposure in heavy metal elements of children and analyzes high-risk factors of the heavy metal element exposure of the children, thereby laying a foundation for further controlling pollution and reducing the harm of environmental pollution to the growth and development of the children.
The invention provides a method for detecting essential trace elements of human blood based on scientific research application, which comprises the following steps:
s1: selecting standard solutions of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium;
s2: diluting standard solutions of vanadium, chromium, manganese, cobalt, nickel and copper molybdenum into 0.0,0.1, 0.5, 1.0, 2.5, 5.0, 10, 20, 50 and 75ng/mL through a diluent; diluting standard solutions of iron, zinc and selenium into 0.0,1, 5, 10, 25, 50, 100, 200, 500 and 750ng/mL by using a diluent;
s3: drawing a standard curve according to the concentration value in the step S2;
s4: placing the diluted standard solution in the step S2 into an instrument for detection, and generating a blood essential trace element detection and measurement formula;
s5: taking out the blood sample from the refrigerator at-80 ℃ and thawing the blood sample in the refrigerator at 4 ℃;
s6: after the sample is thawed, mixing the blood sample uniformly, sucking 200 microliters, adding 3.8 milliliters of diluent, and mixing uniformly to form a sample to be detected;
s7: leading the sample to be detected in the step S6 into an instrument, and detecting and calculating essential trace elements of human blood by using a blood essential trace element detection and measurement formula in the step S4;
s8: after the measurement is completed, a count value of the sample is obtained, and a result is generated.
Further, the diluents in the step S2 and the step S6 are water and NH 4 OH, isopropanol, EDTA, triton X-100.
Further, the ratio of each component of the diluent is 94.98 percent of water and 1 percent of NH respectively 4 OH, 4% isopropanol, 0.01% EDTA, 0.01% Triton X-100.
Further, the step S8 further includes selecting a standard substance as an internal standard element, adding the standard substance, a blank sample and a parallel sample into each batch of samples for quality control, performing instrument inspection on the prepared samples, blank samples and standard substances, testing each sample for 3 times, and taking an average value.
Further, the step S8 further includes monitoring and examining the results, including maintaining examination of the results of the internal standard, standard quality control and human mixed whole blood sample during the detection process, and retesting and analyzing the anomalies in the results and anomalies in the sample detection results.
Further, the concentration of the sample to be detected in the step S6 is within the standard curve range.
Further, the standard curve gamma>0.995, a quantitative detection limit of 10σ/S A Sigma is 11 times reagentStandard deviation of blank solution, S A For sensitivity, calculating the formula (S-B)/C; and S is a response value of a certain standard solution element measured in three times in parallel, B is a response value of a blank solution element, C is the concentration of a certain standard solution, and the detection limit is calculated and finally needs to be multiplied by the dilution multiple.
Further, the water, NH 4 OH, isopropanol, EDTA and Triton X-100 are mixed in a PFA bottle to form a diluent.
Further, the step S7 further includes ionizing the sample plasma to be detected, and then implementing detection analysis of the ion beams of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium elements through an ion lens and a four-level rod mass detector.
The invention provides a method for detecting essential trace elements in human blood based on scientific research application, which is used for detecting the essential trace elements in various whole blood in batches, and carrying out investigation on the pollution level of the essential trace elements in children blood by adopting an inductively coupled plasma mass spectrometer (ICP-MS), so as to rapidly screen the lack or excess of the trace elements, and early find early intervention and early treatment; the method can quickly and accurately know the exposure condition of the essential microelements of children in each region, compares with foreign researches, evaluates the current exposure situation of the essential microelements of children in China, analyzes the high-risk factors of lack or excess essential microelements of children, and has important significance for further regulating the balance of the microelements in the children and promoting the long-term development of the growth and development of the children.
Drawings
Fig. 1 is a schematic flow chart of a method for detecting essential trace elements in human blood based on scientific research application according to an embodiment of the invention.
Detailed Description
The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
The terms first, second, third, fourth and the like in the description and in the claims, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order.
Example 1
Fig. 1 is a schematic flow chart of a method for detecting essential trace elements in human blood based on scientific research application according to an embodiment of the invention. Referring to fig. 1, the method for detecting essential trace elements in human blood based on scientific research application provided by the embodiment of the invention is characterized by comprising the following steps:
s1: selecting standard solutions of manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), selenium (Se), molybdenum (Mo), vanadium (V) and chromium (Cr);
s2: diluting standard solutions of vanadium, chromium, manganese, cobalt, nickel and copper molybdenum into 0.0,0.1, 0.5, 1.0, 2.5, 5.0, 10, 20, 50 and 75ng/mL through a diluent; diluting standard solutions of iron, zinc and selenium into 0.0,1, 5, 10, 25, 50, 100, 200, 500 and 750ng/mL by using a diluent;
s3: drawing a standard curve according to the concentration value in the step S2;
s4: placing the diluted standard solution in the step S2 into an instrument for detection, and generating a blood essential trace element detection and measurement formula;
the instrument may be an inductively coupled plasma mass spectrometer (ICP-MS/MS, agilent, 8900);
s5: taking out the blood sample from the refrigerator at-80 ℃ and thawing the blood sample in the refrigerator at 4 ℃;
s6: after the sample is thawed, mixing the blood sample uniformly, sucking 200 microliters, adding 3.8 milliliters of diluent, and mixing uniformly to form a sample to be detected;
specifically, sample preparation to be tested was prepared by taking 10-fold dilution of the sample by an Agilent I-AS autosampler equipped with 89 sample trays (6 ml sample bottles):
s61: the diluent in the step S2 is used when preparing a sample to be detected or a standard solution; the standard solution is mostly an acidic medium, and can generate precipitation under the alkaline condition with high pH value, and in order to avoid the generation of the precipitation, the standard solution with middle concentration is prepared in advance; the prepared intermediate concentration standard solution is 10 times of the final use concentration (for example, 5ppb standard solution, the intermediate concentration is 50 ppb), and no internal standard element is added into the standard solution;
s62: adding 1/2 bottle volume of diluent to the washed 250ml bottle, and then adding internal standard element solution (the concentration of the internal standard element is 2 times of the final use concentration, and 1000ppm of internal standard element is used for preparation in order to reduce the introduction of acid solution); the solution will be diluted 2-fold for use and the final volume will reach 500ml (100 samples or standard solutions can be processed); diluent is used to fix volume to corresponding scale.
S63: a disposable plastic pipette was used to aspirate 0.5ml of sample or standard solution into the I-AS sample tube and 0.5ml of diluent was aspirated AS blank (no internal standard solution was added).
S64: 2.5ml of the internal standard solution (configured in step S62) was aspirated with a 5ml pipette and added to sample bottles of the sample and standard solution, respectively; this step adds internal standard elements and diluents to the sample or standard solution.
S65: 2ml of water was aspirated into each sample bottle and the final volume in the sample tube was 5ml, and 10-fold dilutions of both sample and standard solution were made.
The upper part of the sample is added with a diluting agent and water with increased volumes, and the mixture is required. Ultrasonic mixing can be used if necessary, and the sample that is prone to coagulation requires ultrasonic treatment.
S7: leading the sample to be detected in the step S6 into an instrument, and detecting and calculating essential trace elements of human blood by using a blood essential trace element detection and measurement formula in the step S4;
it should be noted that, the step S7 further includes that after ionization, the ion beam detection and analysis of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium (m/z) are realized through an ion lens and a four-level rod mass detector in the sample plasma to be detected; the count value/internal standard count value is respectively proportional to the concentration of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium, and is compared and quantified with a standard series;
s8: after the measurement is completed, a count value of the sample is obtained, and a result is generated.
Further, the diluent in the step S2 and the step S6 is a mixed solution of water, NH4OH, isopropanol, EDTA and Triton X-100; specifically, the water, NH4OH, isopropanol, EDTA and Triton X-100 are mixed in a PFA bottle to form a diluent (the high density polyester bottle cannot be used because it contains a filler material that may cause contamination).
Further, the diluent is uniformly mixed by ultrasonic treatment; the ultrasonic treatment not only accelerates the mixing process, but also reduces the possibility of bubbles generated by dissolved gas during sample processing.
The ratio of each component of the diluent is 94.98% of water and 1% of NH 4 OH, 4% isopropanol, 0.01% EDTA, 0.01% Triton X-100.
For example, the components are mixed in the following table, calculated by weight of each component to a final volume of 1L:
specifically, water: the main solvent, base diluent, ultrapure grade, prepared using Milli-Q or Elga ultra-pure water systems (conductivity > 18mΩ);
NH 4 OH: an alkaline reagent to lyse cells and prevent protein precipitation; high purity or ultra high purity grade;
isopropyl alcohol: as a standard solution and a carbon matrix matching solution of blood samples, the accuracy of the measured value of the high ionization energy element is ensured, and the sensitivity of the high ionization energy element can be improved; high purity or ultra high purity grade;
EDTA: complexing agents, which make the metal more stable under alkaline conditions, are used to select high-purity ethylenediamine tetraacetic acid (EDTA), and EDTA sodium salt is not used;
triton X-100: the nonionic surfactant improves the viscosity and the solubility of the sample, and reduces the precipitation and the blockage of the sample to the atomizing chamber and the rectangular tube central tube; promote cell lysis and ensure the stability of protein and fat in the solution.
Further, the step S8 further includes selecting a standard substance as an internal standard element, adding the standard substance, a blank sample and a parallel sample into each batch of samples for quality control, performing instrument inspection on the prepared samples, blank samples and standard substances, testing each sample for 3 times, and taking an average value.
The step S8 further includes monitoring and examining the results, including maintaining examination of the results of the internal standard, standard quality control and human mixed whole blood sample during the detection process, and retesting and analyzing the results and sample detection results when anomalies occur.
Further, the concentration of the sample to be detected in the step S6 is within the standard curve range.
The standard curve gamma>0.995, a quantitative detection limit of 10σ/S A Sigma is the standard deviation of 11 reagent blank solutions, S A For sensitivity, calculating the formula (S-B)/C; and S is a response value of a certain standard solution element measured in three times in parallel, B is a response value of a blank solution element, C is the concentration of a certain standard solution, and the detection limit is calculated and finally needs to be multiplied by the dilution multiple.
Based on the above description, the invention has the advantages that:
according to the method for detecting the essential trace elements in the human blood based on scientific research application, which is provided by the invention, the essential trace elements in the whole blood are detected in batches, and the pollution level of the essential trace elements in the blood of children is investigated by adopting an inductively coupled plasma mass spectrometer (ICP-MS), so that the lack or excess of the trace elements is rapidly screened, and early intervention and early treatment are found; the method can quickly and accurately know the exposure condition of the essential microelements of children in each region, compares with foreign researches, evaluates the current exposure situation of the essential microelements of children in China, analyzes the high-risk factors of lack or excess essential microelements of children, and has important significance for further regulating the balance of the microelements in the children and promoting the long-term development of the growth and development of the children.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (9)
1. The method for detecting the essential trace elements of the human blood based on scientific research application is characterized by comprising the following steps of:
s1: selecting standard solutions of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium;
s2: diluting standard solutions of vanadium, chromium, manganese, cobalt, nickel and copper molybdenum into 0.0,0.1, 0.5, 1.0, 2.5, 5.0, 10, 20, 50 and 75ng/mL through a diluent; diluting standard solutions of iron, zinc and selenium into 0.0,1, 5, 10, 25, 50, 100, 200, 500 and 750ng/mL by using a diluent;
s3: drawing a standard curve according to the concentration value in the step S2;
s4: placing the diluted standard solution in the step S2 into an instrument for detection, and generating a blood essential trace element detection and measurement formula;
s5: taking out the blood sample from the refrigerator at-80 ℃ and thawing the blood sample in the refrigerator at 4 ℃;
s6: after the sample is thawed, mixing the blood sample uniformly, sucking 200 microliters, adding 3.8 milliliters of diluent, and mixing uniformly to form a sample to be detected;
s7: leading the sample to be detected in the step S6 into an instrument, and detecting and calculating essential trace elements of human blood by using a blood essential trace element detection and measurement formula in the step S4;
s8: after the measurement is completed, a count value of the sample is obtained, and a result is generated.
2. The method for detecting essential trace elements in human blood based on scientific research application according to claim 1, wherein the diluents in the step S2 and the step S6 are water and NH 4 OH, isopropanol, EDTA,A mixture of Triton X-100.
3. The method for detecting essential trace elements in human blood based on scientific research application according to claim 2, wherein the components of the diluent respectively comprise 94.98% of water and 1% of NH 4 OH, 4% isopropanol, 0.01% EDTA, 0.01% Triton X-100.
4. The method for detecting essential trace elements in human blood based on scientific research application according to claim 1, wherein the step S8 further comprises selecting standard substances as internal standard elements, adding standard substances, blank samples and parallel samples into each batch of samples for quality control, performing instrument detection on the prepared samples, blank samples and standard substances, testing each sample for 3 times, and taking an average value.
5. The method for detecting essential trace elements in human blood based on scientific research application according to claim 4, wherein the step S8 further comprises monitoring and examining the results, wherein the monitoring and examining of the results comprises maintaining examination of the results of internal standard, standard quality control and human mixed whole blood sample during the detection process, and retesting and analyzing the results abnormality and sample detection result abnormality.
6. The method for detecting essential trace elements in human blood based on scientific research application according to claim 1, wherein the concentration of the sample to be detected in step S6 is within the standard curve range.
7. The method for detecting essential trace elements in human blood based on scientific research application according to claim 6, wherein the standard curve gamma>0.995, a quantitative detection limit of 10σ/S A Sigma is the standard deviation of 11 reagent blank solutions, S A For sensitivity, calculating the formula (S-B)/C; wherein S is a response value of a certain standard solution element measured in three times in parallel, B is a response value of a blank solution element, and C is a certain standardThe concentration of quasi-solution, the detection limit, is calculated and finally multiplied by the dilution factor.
8. The method for detecting essential trace elements in human blood based on scientific research application according to claim 2, wherein the method is characterized in that the water and NH 4 OH, isopropanol, EDTA and Triton X-100 are mixed in a PFA bottle to form a diluent.
9. The method for detecting essential trace elements in human blood based on scientific research application according to claim 8, wherein the step S7 further comprises the step of carrying out detection analysis on ion beams of manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, vanadium and chromium elements in the sample plasma to be detected through an ion lens and a four-level rod mass detector after ionization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311855213.8A CN117805217A (en) | 2023-12-29 | 2023-12-29 | Human blood essential trace element detection method based on scientific research application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311855213.8A CN117805217A (en) | 2023-12-29 | 2023-12-29 | Human blood essential trace element detection method based on scientific research application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117805217A true CN117805217A (en) | 2024-04-02 |
Family
ID=90419666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311855213.8A Pending CN117805217A (en) | 2023-12-29 | 2023-12-29 | Human blood essential trace element detection method based on scientific research application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117805217A (en) |
-
2023
- 2023-12-29 CN CN202311855213.8A patent/CN117805217A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108195924B (en) | Inductively coupled plasma mass spectrometry detection kit for elements in whole blood and application | |
| Harrington et al. | Analysis of human serum and whole blood for mineral content by ICP-MS and ICP-OES: development of a mineralomics method | |
| Krachler et al. | Concentrations of selected trace elements in human milk and in infant formulas determined by magnetic sector field inductively coupled plasma-mass spectrometry | |
| CN108593758A (en) | A method of measuring trace metal content in biological tissue and body fluid | |
| CN107024526A (en) | Micro- kit in a kind of measure human urine | |
| CN113866255B (en) | Inductively coupled plasma mass spectrometry detection of 10 elements in peripheral blood | |
| CN111982789B (en) | High-throughput detection method of metal ions and metal nanoparticles based on single-cell enrichment and single-cell mass spectrometry | |
| KR20210078882A (en) | Multi-elemental analysis of blood by inductively coupled plasma/mass spectrometry | |
| CN113984872B (en) | Inductively coupled plasma mass spectrometry detection of 10 elements in peripheral blood | |
| Forrer et al. | Direct determination of selenium and other trace elements in serum samples by ICP-MS | |
| CN113960153A (en) | ICP-MS (inductively coupled plasma-mass spectrometry) detection method for 12 elements in serum | |
| CN117805217A (en) | Human blood essential trace element detection method based on scientific research application | |
| Harrington et al. | Validation of a metallomics analysis of placenta tissue by inductively-coupled plasma mass spectrometry | |
| CN117805219A (en) | Human blood eleven-element detection method based on clinical application | |
| CN113866256B (en) | Inductively coupled plasma mass spectrum detection product for 10 elements in peripheral blood and application | |
| CN117805218A (en) | Human blood harmful metal element detection method based on scientific research application | |
| CN113237942B (en) | Method for detecting multiple microelements in microelements and application thereof | |
| CN108469419A (en) | The method that IgG content is detected using microplate reader Immunity transmission turbidity high throughput | |
| CN117805216A (en) | Human blood rare earth element detection method based on scientific research application | |
| CN110987565A (en) | Method for quantitatively detecting serum elements by inductively coupled plasma mass spectrometry | |
| Wah Fong et al. | Multi-elements (aluminium, copper, magnesium, manganese, selenium and zinc) determination in serum by dynamic reaction cell-inductively coupled plasma-mass spectrometry | |
| CN116106293A (en) | Method for simultaneously detecting multiple chemical elements on single cell | |
| CN110389116A (en) | A kind of N-S/CDs-Fe3+System studies the process for selective of pyrophosphate ion (PPI) | |
| CN111175402B (en) | Qualitative and quantitative analysis method for selenite and sodium selenite in selenium-containing product | |
| Cao et al. | Detection of inorganic elements in single boar spermatozoa by inductively coupled plasma mass spectrometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |