CN117801988B - Lactobacillus mucilaginosus DP116 and application thereof in preparing products with functions of improving and protecting basement membrane - Google Patents
Lactobacillus mucilaginosus DP116 and application thereof in preparing products with functions of improving and protecting basement membrane Download PDFInfo
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A lactobacillus mucilaginosus DP116 and application thereof in preparing products with the functions of improving and protecting basement membrane belong to the technical field of microorganisms. The lactobacillus mucilaginosus DP116 of the invention is preserved in China center for type culture Collection, with the preservation number of: cctccc NO: m20231972. The use of the lactobacillus mucilaginosus DP116 of the present invention for preparing a product having the function of improving and protecting a basement membrane. The post-element suspension of the fermented lactobacillus mucilaginosus DP116 is taken daily or the post-element gel of the fermented lactobacillus mucilaginosus DP116 is smeared, so that the damage of ultraviolet radiation to the basal membrane of the skin can be effectively prevented and relieved, the skin elasticity is improved, the HYP level in the basal membrane is improved, the pathological damage of the ultraviolet radiation to the basal membrane is relieved, and the expression level of laminin protein and EGF protein in the basal membrane is improved.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 and application thereof in preparing products with the functions of improving and protecting basement membrane.
Background
Repeated exposure to UVB (ultraviolet radiation b) radiation can damage the skin, initially causing premature wrinkling, dryness, thinning and increased pigmentation of the skin, resulting in photoaging of the skin. Increased ROS (reactive oxygen species) generated by uv irradiation not only induces cell death, but also increases the expression level of matrix metalloproteinases, thereby inducing skin to appear as erythema, edema, hyperplasia, inflammatory cell infiltration, immunosuppression, and severe skin malignancy. Therefore, researchers are working to find important methods for preventing, delaying and treating skin damage and skin diseases caused by ultraviolet rays.
The basal membrane is positioned at the lower protruding part of the epidermis and is connected with the papilla of the dermis layer in a wavy shape. The basement membrane is an important component of the skin and provides a structural and functional basis for the skin. The protective substrate film helps to maintain the integrity and health of the skin, preventing skin from drying out, sagging, and aging. The permeable barrier of the basement membrane prevents the ingress of harmful substances into the skin, moisture loss and the ingress of external irritants. The protective substrate film can reduce the chance of skin damage caused by ultraviolet rays, avoid excessive or insufficient pigmentation, and keep the skin natural and uniform in color. Meanwhile, the basement membrane is an important link for repairing damaged skin, and the basement membrane is protected, so that the self-repairing capability of the skin can be promoted, and the damaged skin is helped to recover the normal structure and function. Thus, the function of the base film is critical.
The probiotics have good protective effect on skin. For example, probiotics can generate antioxidant substances, can effectively remove free radicals in vivo, can help to maintain balance of intestinal flora, reduce damage to skin caused by harmful substances and toxins entering blood, can generate various anti-inflammatory substances, and can relieve discomfort symptoms such as skin inflammation, red swelling and the like. The prebiotics are produced by culturing, fermenting, inactivating and lysing specific probiotics, are a kind of 'inanimate symbiotic bacteria, cell-free supernatant and key components beneficial to host health', and are bioactive microorganism metabolites or other effective components. The metaplasia has wide application in the fields of agriculture, industry, environmental protection, food processing and the like. The use of prebiotics made from probiotics to protect the basal lamina in the skin would therefore be a new strategy to prevent and treat skin damage caused by UVB irradiation.
Disclosure of Invention
The invention aims to provide lactobacillus fermentum DP116 and application thereof in preparing products with the function of improving and protecting a substrate film, which are used for improving and protecting the substrate film.
The technical scheme adopted by the invention for solving the technical problems is as follows:
The lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 of the invention is preserved in China center for type culture Collection, with the preservation number of: cctccc NO: m20231972.
The use of a strain of lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 according to the present invention for the preparation of a product having the function of improving and protecting a basement membrane.
As a preferred embodiment, the product is a pharmaceutical or cosmetic product.
As a preferred embodiment, the lactobacillus fermentum DP116 is prepared into lactobacillus fermentum DP116 metazoan freeze-dried powder, and then the lactobacillus fermentum DP116 metazoan freeze-dried powder is dissolved in sterile physiological saline to prepare lactobacillus fermentum DP116 metazoan suspension.
As a preferred embodiment, the lactobacillus fermentum DP116 is prepared into lactobacillus fermentum DP116 metazoan freeze-dried powder, and then the lactobacillus fermentum DP116 metazoan freeze-dried powder is dissolved in propylene glycol to prepare lactobacillus fermentum DP116 metazoan gel.
The beneficial effects of the invention are as follows:
The invention provides a lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 which is preserved in China Center for Type Culture Collection (CCTCC) for short in the year 2023, 10 and 20, and has the following addresses: in the Jiuqiu No. 299 university of Wuhan in Wuchang district of Wuhan, hubei province (Wuhan university collection), the preservation number is: cctccc NO: m20231972.
Experiments prove that the daily intake of the post-metasuspension of the fermentation lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 or the application of the post-metagel of the fermentation lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 can effectively prevent and relieve the damage of ultraviolet radiation to the basal lamina of the skin, improve the skin elasticity, improve the level of Hydroxyproline (HYP) in the basal lamina, relieve the pathological damage of ultraviolet radiation to the basal lamina, and improve the expression level of laminin protein and EGF protein in the basal lamina. The invention provides a new strategy for preventing and treating skin damage repair caused by UVB irradiation.
Drawings
FIG. 1 shows the results of skin elasticity measurement of mice.
Fig. 2 shows the results of Hydroxyproline (HYP) content measurement in the basal membrane of mice.
Fig. 3 is a change in the thickness of the mouse basal membrane.
FIG. 4 shows the expression of laminin protein in basement membrane.
FIG. 5 shows the positive area ratio of laminin protein expression in basement membrane.
FIG. 6 shows the effect of the post-natal of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 on EGF protein expression in the basement membrane.
Detailed Description
The invention provides a lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 which is preserved in China Center for Type Culture Collection (CCTCC) for short in the year 2023, 10 and 20, and has the following addresses: in the Jiuqiu No. 299 university of Wuhan in Wuchang district of Wuhan, hubei province (Wuhan university collection), the preservation number is: cctccc NO: m20231972.
The use of a strain of lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 according to the present invention for the preparation of a product having the function of improving and protecting a basement membrane.
Preferably, the product is a pharmaceutical or cosmetic product, but is not limited thereto.
Preferably, the lactobacillus fermentum DP116 is prepared into lactobacillus fermentum DP116 metagen freeze-dried powder, and then the lactobacillus fermentum DP116 metagen freeze-dried powder is dissolved in sterile physiological saline to prepare lactobacillus fermentum DP116 metagen suspension, but is not limited thereto.
Preferably, the lactobacillus fermentum DP116 is prepared into lactobacillus fermentum DP116 metagen freeze-dried powder, and then the lactobacillus fermentum DP116 metagen freeze-dried powder is dissolved in propylene glycol to prepare lactobacillus fermentum DP116 metagen gel, but is not limited thereto.
The lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 provided by the invention has the following functions:
(a1) Improving skin elasticity;
(a2) Increasing the level of Hydroxyproline (HYP) in the basement membrane;
(a3) Relieving pathological damage of ultraviolet radiation to the basement membrane;
(a4) Increasing the expression level of laminin protein in the basement membrane;
(a5) The expression level of EGF protein in the basement membrane is increased.
The technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The test materials used in the examples below, unless otherwise specified, were purchased from conventional biochemicals. The quantitative tests in the following examples were all set up in triplicate and the results averaged.
Example 1 isolation and identification and preservation of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116
1. Isolation of strains
At the end of 10 months 2022, fresh pickled Chinese cabbage samples were collected from the general market of Jilin province and placed in transport medium, quickly placed in ice boxes and sent to the laboratory for strain isolation. Homogenizing the pickled Chinese cabbage sample with a homogenizer under aseptic condition, subjecting the homogenate to gradient dilution under aseptic condition, selecting homogenate with proper dilution, uniformly coating the homogenate in a solid MRS culture medium, culturing at 37 ℃ for 48-72 h, picking typical single colony (round, neat in edge, milky white, glossy and opaque) and streaking on the solid MRS culture medium for continuous culture, and performing streaking culture and purification for multiple times to obtain pure colonies. The pure cultured strain is inoculated into a liquid MRS culture medium for culture, then prepared into a glycerol pipe, and then placed in a-80 refrigerator for preservation. The obtained strain was designated as DP116.
The preparation method of the adopted solid MRS culture medium comprises the following steps:
The solvent is tertiary water, peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, KH 2PO4 g/L, sodium acetate 5g/L, sodium citrate 5g/L, mgSO 4·7H2O 0.2g/L、MnSO4·4H2 O0.05 g/L, tween-80 1mL/L, glucose 20g/L, agar 15g/L, and pH=6.6.
2. Identification of strains
1. Physiological and biochemical identification results:
The strain DP116 is dyed by a gram-dyeing method and observed, and the strain DP116 is found to be gram-positive and is arranged in short rods and short chains, does not move and has no spores. 50uL of the culture solution of the strain DP116 was directly added dropwise to 3% hydrogen peroxide, and the contact enzyme reaction of the strain was immediately observed, and no air bubbles were generated, so that it was judged that the strain DP116 was negative for contact enzyme.
According to the growth condition of the strain in the solid culture medium and the colony morphology, and combining gram staining and a contact enzyme reaction test result, the strain DP116 is primarily judged to be lactobacillus.
2. 16S rDNA sequence homology analysis:
Bacterial strain DP116 was inoculated into liquid MRS medium (the difference between liquid MRS medium and solid MRS medium is only that agar was not added), after culturing at 37℃for 16h, the genome was extracted using bacterial genome extraction kit, PCR amplification was performed using bacterial 16S primer (27F: AGAGAGTTTGATCCTGGCTCAG; 1492R: TACGGCTACCTTGTTACGACTT), and the amplified product was sent to Beijing Liuhua macrogene technologies Co., ltd. For sequencing, and the obtained 16S rDNA sequence was shown as SEQ ID NO: 1. The 16s rDNA sequence was subjected to homology alignment analysis in GenBank database by BLAST program, and it was found that the strain DP116 had 99.3% homology with Limosilactobacillus fermentum strain MG4263 (GenBank: OP 102570.1) and Limosilactobacillus fermentum strain B152 (GenBank: OM 948682.1).
Based on the above identification, strain DP116 was identified as Lactobacillus fermentum (Limosilactobacillus fermentum).
3. Preservation of strains
The invention discloses a lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 strain which is preserved in China Center for Type Culture Collection (CCTCC) for short, and has the following addresses: in the Jiuqiu No. 299 university of Wuhan in Wuchang district of Wuhan, hubei province (Wuhan university collection), the preservation number is: cctccc NO: m20231972.
Example 2 preparation of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoan
Lactobacillus fermentum (Limosilactobacillus fermentum) DP116 was activated and then activated lactobacillus fermentum (Limosilactobacillus fermentum) DP116 was inoculated into 500mL liquid MRS medium and incubated at 37 ℃ for 16-18 h. The cultured bacterial liquid is centrifuged at 6000 r/min and 4 ℃ for 10: 10 min, the supernatant is discarded, bacterial mud is dissolved in sterile normal saline, and the supernatant is discarded after centrifugation. Collecting bacterial mud, adding 50mL sterile normal saline, repeatedly freezing and thawing for 3 times in a water bath at 37 ℃ and liquid nitrogen, and performing wall breaking treatment under ice bath condition by using ultrasonic waves (power 800 w and ultrasonic waves 15 min). Lyophilizing with vacuum lyophilizing machine (pre-freezing temperature-80deg.C, pre-freezing time 4h, material thickness 1 cm, cold trap temperature-55deg.C, drying chamber vacuum degree 5 Pa, and lyophilizing time 36 h) to obtain lyophilized powder of fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP 116.
Test example 1 protection of the post-metazoan of Lactobacillus mucilaginous (Limosilactobacillus fermentum) DP116 against damage to the basal lamina induced by ultraviolet radiation
1. Preparation of a suspension of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoan
Taking 40 ug fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoan freeze-dried powder, dissolving in1 mL sterile normal saline, and preparing the fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoan suspension.
2. Preparation of fermented Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metagel
40 Ug fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metaplasia freeze-dried powder is taken and dissolved in1 mL propylene glycol, and the mixture is fully and uniformly mixed to prepare the fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metaplasia gel.
3. Packet processing
SPF-grade healthy male C57bl/6 mice purchased from vinca Yisi laboratory animal technologies Co., ltd., production license number: SCXK (Ji) -2011-0004. The animals are fed adaptively for one week, the temperature of the animal house is 23+/-2 ℃, the relative humidity is (40+/-10)%, and feeding and drinking are carried out freely.
40 Male SPF-grade healthy male C57bl/6 mice were randomly divided into 4 groups of 10. The treatment is as follows:
all test mice were depilated at the same back selected and left with exposed skin in a 3cm area. The specific method is that every 2 days, the mice of each experimental group select the same parts on the back to remove surface hair by using an animal hair shaver, the shaving area is about 3cm multiplied by 3cm, and then animal hair removal cream is used to remove surface fluff.
Control group: basal feed (purchased from vinci laboratory animal technologies) was administered for 8 consecutive weeks, and physiological saline (single administration amount of 0.2 mL) was infused once daily, and propylene glycol was applied to the back skin.
Model group: basal feed (purchased from vinci laboratory animal technologies limited) was administered for 8 consecutive weeks and irradiated every other day. Before irradiation, a UVB lamp tube is preheated by 10 min, the ultraviolet radiation intensity of a light source is measured by an ultraviolet administration dosimeter at intervals of 1-2 min, after the light source is stable, an experimental mouse is placed into a self-made mouse cage and a box irradiation box, and the minimum erythema dose increasing method is selected for irradiation: 1-4 times 100 mj/cm 2, 5-8 times 200 mj/cm 2, 9-12 times 300 mj/cm 2, 13-16 times 400 mj/cm 2, and maintaining the dose of 400 mj/cm 2 until the end of the experiment, the total dose of radiation reaches 7.2 j/cm 2. Physiological saline (single administration amount of 0.2. 0.2 mL) was infused once per day 2h before UVB irradiation, and 0.2. 0.2 mL propylene glycol was applied to the back skin. Mice were sacrificed at the end of the trial.
Smearing group: basal feed (purchased from vinci laboratory animal technologies limited) was administered for 8 consecutive weeks and irradiated every other day, and the molding method was the same as that of the model group. The back skin was smeared with fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metagel (single administration amount of 0.2 mL, equivalent to 2.0×10 7 CFU fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP 116) before UVB irradiation of 2h per day.
Gastric lavage group: basal feed (purchased from vinci laboratory animal technologies limited) was administered for 8 consecutive weeks and irradiated every other day, and the molding method was the same as that of the model group. A post-natal suspension of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 was fermented once per day 2h lavage before UVB irradiation (single administration amount was 0.2 mL, equivalent to that containing 2.0X10 7 CFU of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP 116).
After the end of the test, all mice were sacrificed by cervical dislocation. Collecting skin at back irradiation place, and storing at-80deg.C for biochemical analysis, pathological staining and Western-blot detection.
4. Effect of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoans on skin elasticity in mice
Skin elasticity is closely related to the basement membrane. The skin elasticity of mice was evaluated according to the skin elasticity test method (skin lifting test) established by Tsukahara et al, and the measurement results of the skin elasticity of each group of mice are shown in fig. 1. After 8 weeks of ultraviolet irradiation, the back skin of each test group was gently lifted by thumb and forefinger along the backward position of the median line (in the sense that the limbs of the mice were not suspended), and the time for the skin to recover to the initial state was recorded, and as a result, the recovery time of the model group was found to be the longest, 5.46±0.45 min, and the difference was extremely remarkable (P < 0.01) compared with the control group, indicating that the elasticity was remarkably reduced due to the long-time ultraviolet irradiation, and the skin of the mice was relaxed. After the post-primordial intervention of DP116 of lactobacillus fermentum (Limosilactobacillus fermentum), the skin recovery time is obviously shortened, namely 37.2 percent and 37.92 percent respectively compared with a model group, and the difference is extremely obvious (P < 0.01) compared with the model group, which indicates that the post-primordial intervention of DP116 of lactobacillus fermentum (Limosilactobacillus fermentum) relieves the skin relaxation problem induced by ultraviolet irradiation and also laterally indicates that the damage of ultraviolet irradiation to a basement membrane is relieved after the post-primordial intervention of DP116 of lactobacillus fermentum (Limosilactobacillus fermentum).
5. Effect of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metants on HYP concentration in basement membrane
The Hydroxyproline (HYP) content in the basal lamina of the skin of the mice is measured by using a Nanjing-made Hydroxyproline (HYP) measuring kit (alkaline hydrolysis method) (product number: A030-2-1). Specifically, according to the method provided by the kit for measuring Hydroxyproline (HYP) built in Nanjing, skin at the ultraviolet irradiation position is treated, and the content of Hydroxyproline (HYP) in the basement membrane is detected, and the result is shown in FIG. 2. Hydroxyproline (HYP) is a constituent of collagen, which is one of the main components of the basement membrane, and thus plays an important role in the basement membrane, such as maintaining the structural integrity of the basement membrane, promoting cell interactions with the basement membrane, and participating in repair and regeneration of the basement membrane, etc. After long-term uv irradiation of the skin, the Hydroxyproline (HYP) content in the basement membrane was significantly reduced, with a very significant difference (P < 0.01) compared to the control group. And after the fermentation lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 is given, the content of Hydroxyproline (HYP) is obviously improved, compared with a model group, the difference is obvious (P < 0.05/P < 0.01), and the effect of the smear group is better than that of the gastric lavage group. Indicating that post-natal application or gastric lavage intervention of lactobacillus fermentum (Limosilactobacillus fermentum) DP116 increases Hydroxyproline (HYP) content in the basement membrane.
6. Effect of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metazoans on pathological changes in the basement membrane
Accumulation of uv radiation can cause gradual degradation of the reticular fibers in the basement membrane, rendering the skin fragile and vulnerable. The thickness of the basal lamina was significantly changed after the mice were irradiated with ultraviolet light for a long period of time, and the results are shown in fig. 3. The basal membrane thickness of the mice in the model group was significantly thinned to 0.055±0.001 mm, and the difference was extremely significant (P < 0.01) compared to the control group. Whereas, after the post-natal application and gastric lavage of lactobacillus fermentum (Limosilactobacillus fermentum) DP116, the thickness of the basal lamina became thicker, 0.014±0.001 mm and 0.108±0.001 mm, respectively, and the difference was very significant (P < 0.01) compared to the model group. Indicating that the post-natal application or gastric lavage of lactobacillus fermentum (Limosilactobacillus fermentum) DP116 resulted in relief of the damage to the basement membrane caused by uv irradiation.
7. Effect of fermentation of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metants on immunohistochemistry of laminin protein in basement membrane
Laminin is an important extracellular matrix protein, and excessive ultraviolet irradiation may cause crosslinking and degradation of the laminin protein, so that the laminin protein expression is negatively affected to reduce the function and stability of the laminin protein in a basement membrane. In addition, ultraviolet radiation may also induce conformational changes in the laminin protein, affecting its interaction with skin cells and signal transduction. The immune expression of laminin protein in the basement membrane of mice after long-term ultraviolet irradiation is shown in fig. 4. As can be seen from fig. 4, the laminin protein is mainly expressed in cytoplasm, and the expression level of the laminin protein is remarkably reduced after a certain period of ultraviolet irradiation. As can be seen by analyzing the Positive Area ratio (Positive Area,%) of laminin protein expression (fig. 5), the Positive Area of laminin protein expression of the model group only occupies 22.11±2.18% of the tissue Area, and the difference is very significant (P < 0.01) compared with the control group, which indicates that the laminin protein in the basement membrane is degraded due to ultraviolet irradiation, and the content is reduced. And after the fermentation lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metaplasia and the stomach-filling stem prognosis are given, the expression quantity of the laminin protein is obviously increased, namely 45.23 +/-1.85% and 31.66 +/-1.42%, the difference is extremely obvious compared with a model group (P < 0.01), and the effect of the smear group is better than that of the stomach-filling group. Indicating that the post-primordial smearing or gastric lavage of the fermented lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 resulted in relief of damage to laminin protein in the basement membrane from ultraviolet irradiation.
8. Effect of Lactobacillus mucilaginosus (Limosilactobacillus fermentum) DP116 metants on EGF protein expression in basement membrane
Excessive uv irradiation may cause excessive apoptosis of epidermal cells, thereby reducing synthesis and release of Epidermal Growth Factor (EGF). As a result, as shown in fig. 6, the expression amount of EGF protein was significantly reduced in the basal membrane of the mice of the model group, and the difference was extremely significant (P < 0.01) compared with the control group. Whereas the expression level of EGF protein was significantly increased after post-metaplasia and gastric lavage stem prognosis given to Lactobacillus fermentum (Limosilactobacillus fermentum) DP116, the differences were very significant compared to the model group (P < 0.01). Indicating that administration of lactobacillus fermentum (Limosilactobacillus fermentum) DP116 post-natal smear or lavage intervention increased expression of EGF protein in the basement membrane.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (5)
1. A lactobacillus fermentum (Limosilactobacillus fermentum) DP116, which is deposited with the chinese collection for typical cultures at 10 and 20 of 2023 under the accession number: cctccc NO: m20231972.
2. Use of a strain of lactobacillus fermentum (Limosilactobacillus fermentum) DP116 as claimed in claim 1 in the manufacture of a product for alleviating damage to the basal lamina of skin caused by uv irradiation.
3. Use according to claim 2, characterized in that the product is a pharmaceutical or cosmetic product.
4. The use according to claim 2, wherein the lactobacillus fermentum DP116 is prepared as a post-cursor freeze-dried powder of lactobacillus fermentum DP116, and the post-cursor freeze-dried powder of lactobacillus fermentum DP116 is dissolved in sterile physiological saline to prepare a post-cursor suspension of lactobacillus fermentum DP 116.
5. The use according to claim 2, wherein the lactobacillus fermentum DP116 is prepared as a post-cursor freeze-dried powder of lactobacillus fermentum DP116, and the post-cursor freeze-dried powder of lactobacillus fermentum DP116 is dissolved in propylene glycol to prepare a post-cursor gel of lactobacillus fermentum DP 116.
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