CN117797316B - Electrically-induced medical nerve repair material and manufacturing method thereof - Google Patents
Electrically-induced medical nerve repair material and manufacturing method thereof Download PDFInfo
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Abstract
The invention discloses an electrically-induced medical nerve repair material and a manufacturing method thereof, and relates to the technical field of medical appliances, wherein the electrically-induced medical nerve repair material comprises the following components: the main body structure is used for providing space for nerve repair and is made of one or more of chitosan, chitin, sodium alginate and gelatin; the conductive structure is used for adsorbing nerves and is fixedly arranged on the main structure and comprises first conductive fibers and second conductive fibers, wherein the first conductive fibers and the second conductive fibers are different in conductivity, the conductive structure is made of a mixture of reduced graphene oxide, polycaprolactone and polyvinylpyrrolidone, and the sensory nerves and the motor nerves are respectively climbed on the corresponding conductive fibers under the action of electric induction, so that the defect that the nerve functions of the motor nerves and the sensory nerves cannot be completely repaired due to misconnection is avoided, and the repairing effect is improved; the nerve signals can be conducted while the nerve climbs, the nerve function is recovered, and the repair efficiency is improved.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to an electrically-induced medical nerve repair material and a manufacturing method thereof.
Background
At present, the repairing modes of the nerve defect mainly comprise direct suturing, adhesive bonding, sleeve suturing and the like. In the repairing process, the nerve function is unavailable, the nerve function cannot be recovered to be normal until the nerve function is completely repaired, the repairing efficiency is low, and the motor nerve and the sensory nerve can possibly generate the defect that the nerve function cannot be completely repaired due to misconnection, so that the repairing effect is poor.
Disclosure of Invention
In order to overcome the defects of the prior art, the embodiment of the invention provides an electrically-induced medical nerve repair material and a manufacturing method thereof. The embodiment of the invention provides an electrically-induced medical nerve repair material and a manufacturing method thereof, and adopts the following technical scheme:
in a first aspect, an embodiment of the present invention provides an electrically-induced medical nerve repair material comprising:
The main body structure is used for providing space for nerve repair and is made of one or more of chitosan, chitin, sodium alginate and gelatin.
The conductive structure is used for adsorbing nerves and is fixedly arranged on the main structure and comprises first conductive fibers and second conductive fibers, wherein the first conductive fibers and the second conductive fibers are different in conductivity, and the conductive structure is made of a mixture of reduced graphene oxide, polycaprolactone and polyvinylpyrrolidone.
In some examples, the body structure is tubular.
In some examples, the first conductive fibers and the second conductive fibers are sequentially and alternately inlaid in parallel on the inner wall of the main body structure.
In some examples, the body structure is sheet-like.
In some examples, the first conductive fibers and the second conductive fibers are sequentially and alternately inlaid in parallel on one side of the main body structure.
In some examples, the first conductive fiber is used to adsorb sensory nerves, and the second conductive fiber is used to adsorb motor nerves, and the sensory nerves and the motor nerves are respectively climbed onto the corresponding conductive fibers through the action of electric induction.
In a second aspect, a method for manufacturing the electrically-induced medical nerve repair material disclosed in the first aspect provided by the embodiment of the invention comprises the following steps:
Step one, preparing spinning solution
And adding a certain mass of reduced graphene oxide powder into a mixed solution of dichloromethane and dimethylformamide to generate reduced graphene oxide suspension.
After sonicating the reduced graphene oxide suspension at a first ambient temperature, polycaprolactone particles and polyvinylpyrrolidone powder are mixed and added to the reduced graphene oxide suspension.
And uniformly stirring to reduce the graphene oxide suspension to generate a spinning solution.
Step two, preparing a first conductive fiber
And injecting the spinning solution into a prefabricated first die at a set flow rate under a first ambient humidity to generate first conductive fibers.
And thirdly, changing the addition amount of polyvinylpyrrolidone under the second environment temperature and the second environment humidity, and repeating the first to the second steps to generate the second conductive fibers.
Step four, preparing injection molding liquid
One or more of chitosan, chitin, sodium alginate and gelatin are added into purified water to generate injection molding liquid.
Step five, preparing the electrically-induced medical nerve repair material
The first conductive fibers and the second conductive fibers are sequentially and alternately fixed in the clamping groove of the prefabricated second die.
Injecting the injection molding liquid into the second mold and performing freeze drying treatment on the injection molding liquid.
And (3) washing the injection molding liquid with the purified water and carrying out freeze drying treatment on the injection molding liquid again to generate the electrically-induced medical nerve repair material.
In some examples, the reduced graphene oxide powder is added in an amount of 0.1% -5% W/W.
In some examples, the volume ratio of the dichloromethane to the dimethylformamide is 1:1-5:1.
In some examples, the polycaprolactone particles are added in an amount of 3% -15% w/V.
In some examples, the polyvinylpyrrolidone is added in an amount of 5% to 10% w/V.
Compared with the prior art, the electrically-induced medical nerve repair material and the manufacturing method thereof provided by the embodiment of the invention have the following beneficial effects:
(1) According to the conduction rate difference of the sensory nerve and the motor nerve, two conductive fibers with different conductivities are provided, so that the sensory nerve and the motor nerve respectively climb to the different conductive fibers to carry out growth repair in an electric induction mode, the defect that the nerve functions of the motor nerve and the sensory nerve cannot be completely repaired due to misconnection is avoided, and the repair effect is improved;
(2) The nerve can be used for conducting nerve signals and recovering nerve functions while climbing, and the nerve repair catheter or the nerve repair membrane can be gradually degraded in the body along with the completion of nerve repair, so that secondary operation is not required to be taken out, and the repair efficiency is improved.
Drawings
FIG. 1 is a schematic perspective view of an electrically-induced medical nerve repair material with a tubular main body structure according to an embodiment of the present invention;
fig. 2 is a schematic perspective view of an electrically-induced medical nerve repair material with a sheet-shaped main body structure according to an embodiment of the present invention.
FIG. 3 is a schematic plan view of an electrically-induced medical nerve repair material with a tubular main body structure according to an embodiment of the present invention;
Fig. 4 is a schematic plan view of an electrically-induced medical nerve repair material with a sheet-shaped main body structure according to an embodiment of the present invention.
Detailed Description
The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1
The electrically-induced medical nerve repair material provided by the embodiment of the invention comprises a main body structure 1 and a conductive structure (not shown in the figure). Wherein:
The main structure 1 is used for providing space for nerve repair, and is made of one or more of chitosan, chitin, sodium alginate and gelatin.
In particular, the body structure 1 prevents invasion of surrounding scar tissue and provides nutrient exchange for nerve growth.
In some examples, as shown in fig. 1, the body structure 1 is tubular. In this case, the conductive structure is fixedly provided on the inner wall of the main body structure 1.
In some examples, as shown in fig. 2, the body structure 1 is sheet-like. In this case, the conductive structure is fixedly provided on one side of the body structure 1.
The conductive structure is used for adsorbing nerves and is fixedly arranged on the main structure 1 and comprises a first conductive fiber 2 and a second conductive fiber 3, wherein the first conductive fiber 2 and the second conductive fiber 3 are different in conductivity, and the conductive structure is made of a mixture of reduced graphene oxide, polycaprolactone and polyvinylpyrrolidone.
In some examples, the first conductive fiber 2 is used to adsorb sensory nerves and the second conductive fiber 3 is used to adsorb motor nerves. The number of the first conductive fibers 2 and the second conductive fibers 3 may be the same, and may be one or a plurality. In fig. 3 and 4, the number of the first conductive fibers 2 and the second conductive fibers 3 is 4. The conductive structure can adsorb different types of nerves, can quickly recover the nerve function, has higher efficiency, avoids the possibility of misconnection of the motor nerve and the sensory nerve, and has better repairing effect.
Specifically, the diameter of the first conductive fiber 2 and the diameter of the second conductive fiber 3 are about 30 micrometers, wherein, as shown in fig. 3 and 4, the diameter of the first conductive fiber 2 is slightly smaller than the diameter of the second conductive fiber 3, the fiber bundles can be formed by a braiding method, and the electric conduction characteristics of the fiber bundles can be controlled to be matched with the conduction speed of the nerve of 50-70 m/s. According to the difference of conduction speeds of the motor nerve and the sensory nerve, it is necessary to prepare 2 kinds of conductive fibers having conductivity to match the motor nerve and the sensory nerve, respectively.
Specifically, the conductivity of the conductive fiber has a great relationship with the doping level of the solid semiconductor (polyvinylpyrrolidone), the ambient temperature and the ambient humidity. The conductive fibers with different conductivities can be obtained by adjusting the addition amount of the solid semiconductor (polyvinylpyrrolidone), the ambient temperature and the ambient humidity.
In some examples, as shown in fig. 3, the first conductive fibers 2 and the second conductive fibers 3 are sequentially and alternately embedded in parallel on the inner wall of the main body structure 1. In this case, a nerve repair catheter is formed.
In some examples, as shown in fig. 4, the first conductive fibers 2 and the second conductive fibers 3 are sequentially and alternately embedded in parallel on one side surface of the main body structure 1. In this case, a nerve repair film is formed.
Particularly, the types of the conductive fibers in the conductive structure disclosed in the embodiment of the invention are not limited to two types of the first conductive fibers 2 and the second conductive fibers 3, but may be three or more types to repair other types of nerves (such as autonomic nerves and the like).
Example 2
The method for manufacturing the electrically-induced medical nerve repair material provided by the embodiment of the invention comprises the following steps:
Step one, preparing spinning solution
And adding a certain mass of reduced graphene oxide powder into a mixed solution of dichloromethane and dimethylformamide to generate reduced graphene oxide suspension.
In some examples, the reduced graphene oxide powder is added in an amount of 0.1% -5% W/W, with a volume ratio of dichloromethane to dimethylformamide of 1:1-5:1.
After sonicating the reduced graphene oxide suspension at a first ambient temperature, polycaprolactone particles and polyvinylpyrrolidone powder were mixed and added to the reduced graphene oxide suspension.
In some examples, the polycaprolactone particles are added in an amount of 3% -15% w/V and the polyvinylpyrrolidone is added in an amount of 5% -10% w/V.
Specifically, the reduced graphene oxide suspension is sonicated for 30-60 minutes at an ambient temperature of 15 ℃.
And uniformly stirring to reduce the graphene oxide suspension to generate a spinning solution.
Specifically, the reduced graphene oxide suspension was stirred uniformly for 12 hours to prepare a spinning solution.
Step two, preparing a first conductive fiber
Under a first ambient humidity, the spinning solution is injected into a prefabricated first die at a set flow rate to generate first conductive fibers.
Specifically, the diameter of the syringe was set at a position 15cm from the first die at an ambient humidity of 70%, and the spinning solution was sprayed from the syringe at a flow rate of 2.5mL/h to obtain a conductive fiber.
And thirdly, changing the addition amount of polyvinylpyrrolidone under the second environment temperature and the second environment humidity, and repeating the first to the second steps to generate the second conductive fibers.
Step four, preparing injection molding liquid
One or more of chitosan, chitin, sodium alginate and gelatin are added into purified water to generate injection molding liquid.
Step five, preparing the electrically-induced medical nerve repair material
Sequentially and alternately fixing the first conductive fibers and the second conductive fibers in a clamping groove of a prefabricated second die;
Injecting the injection molding liquid into a second mold and performing freeze drying treatment on the injection molding liquid;
and (3) cleaning the injection molding liquid with purified water, and performing freeze drying treatment on the injection molding liquid again to obtain the electrically-induced medical nerve repair material.
Specifically, the application method of the electrically-induced medical nerve repair material provided by the embodiment of the invention comprises the following steps:
depending on the nerve defect site, a nerve repair catheter or a nerve repair membrane is selected. Generally, a single nerve defect site is selected using a nerve repair catheter, and a complex nerve defect site is selected using a nerve repair membrane;
cutting the nerve defect part, treating the nerve broken end, sleeving the treated nerve into a nerve repair catheter or wrapping the nerve repair catheter or the nerve repair membrane, and suturing the nerve repair catheter or the nerve repair membrane;
suturing the outer skin;
Through the electric stimulation mode, the growth of the sensory nerve and the motor nerve is activated, the sensory nerve and the motor nerve can automatically climb on the corresponding conductive fibers, and the conduction function of the nerve signal is recovered after the climbing is successful, so that the nerve function is available in the repairing process, and the efficiency is higher;
the defective nerve grows along the conductive fiber until it is completely repaired;
after the nerve repair is finished, the nerve repair catheter or the nerve repair membrane can be degraded gradually in the body, and is taken out without secondary operation, so that the efficiency is high.
The basic principles of the present disclosure have been described above in connection with specific embodiments, but it should be noted that the advantages, benefits, effects, etc. mentioned in the present disclosure are merely examples and are not to be considered as necessarily possessed by the various embodiments of the present disclosure. Furthermore, the specific details disclosed herein are for purposes of illustration and understanding only, and are not intended to be limiting, since the invention is not necessarily disclosed as being practiced with the specific details.
In this specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different manner from other embodiments, so that the same or similar parts between the embodiments are mutually referred to. For system embodiments, the description is relatively simple as it essentially corresponds to method embodiments, and reference should be made to the description of method embodiments for relevant points.
The block diagrams of the devices, apparatuses, devices, systems referred to in this disclosure are only illustrative examples and are not intended to require or imply that the connections, arrangements, configurations must be made in the manner shown in the block diagrams. As will be appreciated by one of skill in the art, the devices, apparatuses, devices, systems may be connected, arranged, configured in any manner. Words such as "including," "comprising," "having," and the like are words of openness and mean "including but not limited to," and are used interchangeably therewith. The terms "or" and "as used herein refer to and are used interchangeably with the term" and/or "unless the context clearly indicates otherwise. The term "such as" as used herein refers to, and is used interchangeably with, the phrase "such as, but not limited to.
The disclosed methods and apparatus may be implemented in many ways. For example, the methods and apparatus of the present disclosure may be implemented by software, hardware, firmware, or any combination of software, hardware, firmware. The above-described sequence of steps for the method is for illustration only and the steps of the method disclosed herein are not limited to the sequence specifically described above unless specifically stated otherwise. Furthermore, in some embodiments, the present disclosure may also be implemented as programs recorded in a recording medium, the programs including machine-readable instructions for implementing the methods according to the present disclosure. Thus, the present disclosure also covers a recording medium storing a program for executing the method according to the present disclosure.
It is also noted that in the apparatus, devices and methods disclosed herein, components or steps may be separated and/or recombined. Such decomposition and/or recombination should be considered equivalents of the present disclosure. The previous description of the disclosed aspects is provided to enable any person skilled in the art to make or use the present disclosure. Various modifications to these aspects will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other aspects without departing from the scope of the disclosure. Thus, the present disclosure is not intended to be limited to the aspects shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
The foregoing description has been presented for purposes of illustration and description. Furthermore, this description is not intended to limit the disclosed embodiments to the form disclosed herein. Although a number of example aspects and embodiments have been discussed above, a person of ordinary skill in the art will recognize certain variations, modifications, alterations, additions, and subcombinations thereof.
It will be appreciated that the relevant features of the methods and apparatus described above may be referenced to one another. In addition, the "first", "second", and the like in the above embodiments are for distinguishing the embodiments, and do not represent the merits and merits of the embodiments.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and variations of the present application will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the application are to be included in the scope of the claims of the present application.
It should be noted that, the above embodiments are not intended to limit the present invention in any way, and all the technical solutions obtained by adopting equivalent substitution or equivalent transformation fall within the protection scope of the present invention.
Claims (10)
1. An electrically-induced medical nerve repair material, comprising:
the main body structure is used for providing space for nerve repair and is made of one or more of chitosan, chitin, sodium alginate and gelatin;
The conductive structure is used for adsorbing nerves and is fixedly arranged on the main structure and comprises first conductive fibers and second conductive fibers, wherein the first conductive fibers and the second conductive fibers are different in conductivity, and the conductive structure is made of a mixture of reduced graphene oxide, polycaprolactone and polyvinylpyrrolidone.
2. The electrically-induced medical nerve repair material of claim 1, wherein the body structure is tubular.
3. The electrically-induced medical nerve repair material of claim 2, wherein the first conductive fibers and the second conductive fibers are sequentially and alternately inlaid in parallel on the inner wall of the main body structure.
4. The electrically-induced medical nerve repair material of claim 1, wherein the body structure is sheet-like.
5. The electrically-induced medical nerve repair material of claim 4, wherein the first conductive fibers and the second conductive fibers are sequentially embedded in parallel and alternately on one side of the main structure.
6. The electrically-induced medical nerve repair material of any one of claims 1-5, wherein the first conductive fiber is used to adsorb sensory nerves and the second conductive fiber is used to adsorb motor nerves.
7. A method of making the electrically-induced medical nerve repair material of any one of claims 1-6, comprising the steps of:
Step one, preparing spinning solution
Adding a certain mass of reduced graphene oxide powder into a mixed solution of dichloromethane and dimethylformamide to generate reduced graphene oxide suspension;
After performing ultrasonic treatment on the reduced graphene oxide suspension at a first ambient temperature, mixing polycaprolactone particles and polyvinylpyrrolidone powder and adding the mixture into the reduced graphene oxide suspension;
Uniformly stirring and reducing graphene oxide suspension to generate spinning solution;
Step two, preparing a first conductive fiber
Injecting the spinning solution into a prefabricated first die at a set flow rate under a first ambient humidity to generate first conductive fibers;
step three, changing the addition amount of polyvinylpyrrolidone under the second environment temperature and the second environment humidity, and repeating the step one to the step two to generate second conductive fibers;
Step four, preparing injection molding liquid
Adding one or more of chitosan, chitin, sodium alginate and gelatin into purified water to generate injection molding liquid;
step five, preparing the electrically-induced medical nerve repair material
Sequentially and alternately fixing the first conductive fibers and the second conductive fibers in a clamping groove of a prefabricated second die;
injecting the injection molding liquid into the second mold and performing freeze drying treatment on the injection molding liquid;
and (3) washing the injection molding liquid with the purified water and carrying out freeze drying treatment on the injection molding liquid again to generate the electrically-induced medical nerve repair material.
8. The method according to claim 7, wherein the reduced graphene oxide powder is added in an amount of 0.1% -5% W/W.
9. The method according to claim 7, wherein the volume ratio of the dichloromethane to the dimethylformamide is 1:1-5:1.
10. The method of claim 7, wherein the polycaprolactone particles are added in an amount of 3% -15% w/V.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102764479A (en) * | 2012-07-13 | 2012-11-07 | 中国科学院深圳先进技术研究院 | Flexible nerve tract electrode and preparation method thereof |
| CN211634499U (en) * | 2019-12-12 | 2020-10-09 | 天新福（北京）医疗器材股份有限公司 | Patterned graphene nerve conduit |
| CN115975928A (en) * | 2023-02-17 | 2023-04-18 | 西安交通大学医学院第一附属医院 | Electric field force-regulated nerve cell ordered arrangement and nerve channel forming method |
| CN116271246A (en) * | 2023-04-06 | 2023-06-23 | 河北医科大学第三医院 | Reduced graphene oxide nerve conduit and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2006096791A2 (en) * | 2005-03-07 | 2006-09-14 | Georgia Tech Research Corporation | Nanofilament scaffold for tissue regeneration |
| JP6733890B2 (en) * | 2015-04-15 | 2020-08-05 | ラトガース，ザ ステート ユニバーシティ オブ ニュージャージー | Biocompatible implants for nerve regeneration and methods of use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102764479A (en) * | 2012-07-13 | 2012-11-07 | 中国科学院深圳先进技术研究院 | Flexible nerve tract electrode and preparation method thereof |
| CN211634499U (en) * | 2019-12-12 | 2020-10-09 | 天新福（北京）医疗器材股份有限公司 | Patterned graphene nerve conduit |
| CN115975928A (en) * | 2023-02-17 | 2023-04-18 | 西安交通大学医学院第一附属医院 | Electric field force-regulated nerve cell ordered arrangement and nerve channel forming method |
| CN116271246A (en) * | 2023-04-06 | 2023-06-23 | 河北医科大学第三医院 | Reduced graphene oxide nerve conduit and preparation method thereof |
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