CN117796870A - 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis - Google Patents
3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis Download PDFInfo
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- 238000010146 3D printing Methods 0.000 title claims abstract description 64
- 239000000017 hydrogel Substances 0.000 title claims abstract description 55
- 210000004185 liver Anatomy 0.000 title claims abstract description 38
- 230000002792 vascular Effects 0.000 title claims abstract description 35
- 238000002054 transplantation Methods 0.000 title claims abstract description 31
- 230000003872 anastomosis Effects 0.000 title claims abstract description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 43
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 34
- 239000003999 initiator Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 32
- 239000006096 absorbing agent Substances 0.000 claims description 29
- KEROTHRUZYBWCY-UHFFFAOYSA-N tridecyl 2-methylprop-2-enoate Chemical compound CCCCCCCCCCCCCOC(=O)C(C)=C KEROTHRUZYBWCY-UHFFFAOYSA-N 0.000 claims description 27
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 23
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 23
- 238000007639 printing Methods 0.000 claims description 15
- 238000000016 photochemical curing Methods 0.000 claims description 13
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims description 12
- -1 trimethylolpropane ethoxyl ester Chemical class 0.000 claims description 11
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 claims description 10
- 238000002844 melting Methods 0.000 claims description 9
- 230000008018 melting Effects 0.000 claims description 9
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 8
- ZJCCRDAZUWHFQH-UHFFFAOYSA-N Trimethylolpropane Chemical compound CCC(CO)(CO)CO ZJCCRDAZUWHFQH-UHFFFAOYSA-N 0.000 claims description 8
- 238000007605 air drying Methods 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000001723 curing Methods 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 229940032296 ferric chloride Drugs 0.000 claims description 8
- 229940044631 ferric chloride hexahydrate Drugs 0.000 claims description 8
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 8
- NQXWGWZJXJUMQB-UHFFFAOYSA-K iron trichloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].Cl[Fe+]Cl NQXWGWZJXJUMQB-UHFFFAOYSA-K 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
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- 235000005979 Citrus limon Nutrition 0.000 claims description 6
- 244000248349 Citrus limon Species 0.000 claims description 6
- 229940109262 curcumin Drugs 0.000 claims description 6
- 235000012754 curcumin Nutrition 0.000 claims description 6
- 239000004148 curcumin Substances 0.000 claims description 6
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims description 6
- 238000009826 distribution Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 claims description 3
- JUYQFRXNMVWASF-UHFFFAOYSA-M lithium;phenyl-(2,4,6-trimethylbenzoyl)phosphinate Chemical compound [Li+].CC1=CC(C)=CC(C)=C1C(=O)P([O-])(=O)C1=CC=CC=C1 JUYQFRXNMVWASF-UHFFFAOYSA-M 0.000 claims description 3
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- 206010057573 Chronic hepatic failure Diseases 0.000 description 1
- 208000010334 End Stage Liver Disease Diseases 0.000 description 1
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- ANCHPZQGZBCDBK-UHFFFAOYSA-N [PH2](O)=O.C1(=CC=CC=C1)C=1C(=C(C(=O)[Li])C(=CC1C)C)C Chemical compound [PH2](O)=O.C1(=CC=CC=C1)C=1C(=C(C(=O)[Li])C(=CC1C)C)C ANCHPZQGZBCDBK-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
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Abstract
The invention discloses a 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis, which is characterized by comprising a oversleeve body (1), a plurality of tooth strips (2) uniformly distributed on the frame wall of the oversleeve body (1), rotary threads (3) encircling the tooth strips (2) and a positioning clamping arm (4) vertically fixed on the shaft mouth of the oversleeve body (1). According to the invention, the plurality of tooth strips arranged on the oversleeve body are matched with the rotary threads encircling the tooth strips, so that the friction force between the vascular wall and the oversleeve support can be effectively increased, the blood vessel is not easy to fall off from the oversleeve support when being sutured, the blood vessel can be sutured rapidly and effectively, and the occurrence of unsmooth blood flow and even ischemic necrosis of the blood vessel due to eversion for a long time can be well prevented.
Description
Technical Field
The invention relates to the technical field of oversleeve supports, in particular to a 3D printing hydrogel oversleeve support for liver transplantation vascular anastomosis.
Background
Currently, liver transplantation surgery is considered an effective means of treating end-stage liver disease and is widely performed worldwide. However, development of liver transplantation still faces various problems, and thus, establishment of a stable liver transplantation animal model for relevant research has extremely important clinical significance. In the liver transplantation animal model for the related study, since the arterial mouse liver transplantation model has better liver function recovery after operation and is closer to the physiological index of human body, the mouse liver transplantation model is used as the main liver transplantation animal model for the related study. In the liver transplantation model operation of mice, a liver transplantation model combining hepatic artery reconstruction by a oversleeve method is mostly adopted.
However, the existing oversleeve bracket is mainly made of a polymer hollow catheter, and the surface is smooth, so that the vascular epidermis is easy to slip, and the suturing is failed, so that the vascular is everted for a long time, and further, the situations of unsmooth blood flow and even ischemic necrosis of the vascular occur. Meanwhile, the existing polymer oversleeve bracket is poor in manufacturing accuracy, strength and toughness. Therefore, there is a need to develop a oversleeve brace that not only prevents slippage during suturing, but also has high accuracy, strength, and toughness.
Disclosure of Invention
The invention aims to solve the problems and provide the 3D printing hydrogel oversleeve bracket which not only can prevent slipping during suturing, but also has high precision, high strength and high toughness and is used for liver transplantation vascular anastomosis.
The aim of the invention is achieved by the following technical scheme:
the 3D printing hydrogel oversleeve bracket comprises a oversleeve body, a plurality of tooth strips uniformly distributed on the wall of a bracket body of the oversleeve body, rotating threads encircling the tooth strips and positioning clamping arms vertically fixed on the shaft openings of the oversleeve body; the tooth surface of the tooth strip is arc-shaped.
The 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis consists of the following substances in parts by weight: 0.05 to 4 parts of tridecyl methacrylate, 1 to 4 parts of acrylic acid, 1 to 4 parts of acrylamide, 6 to 12 parts of absolute ethyl alcohol, 0.05 to 1 part of trimethylolpropane ethoxyester triacrylate, 0.05 to 1 part of initiator and 0.05 to 1 part of light absorber.
As a preferred embodiment of the present invention, the density of the tridecyl methacrylate is 0.85g/cm 3 ～1g/cm 3 。
As a preferable scheme of the invention, the mass ratio of the acrylic acid to the acrylamide is 1:1; wherein the octanol/water distribution coefficient of the acrylic acid is 0.3-0.05, the viscosity (mPas, 25 ℃) is 1.256 to (mPas, 25 ℃) is 1.5, the relative density (25 ℃ and 4 ℃) is 1.024 to (25 ℃ and 4 ℃) is 1.4.
As a preferred embodiment of the present invention, the acrylamide has a density of 1.322g/cm 3 ～1.4g/cm 3 The melting point was 87 ℃.
Furthermore, the initiator is a cell-compatible I-type photoinitiator of lithium phenyl (2, 4, 6-trimethylbenzoyl) phosphinate, and the purity of the initiator is more than or equal to 98.5 percent.
Further, the light absorber is one or a mixture of more of curcumin, fast green, lemon yellow and sudan.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
s1, adding 0.05-4 parts by weight of tridecyl methacrylate, 1-4 parts by weight of acrylic acid, 1-4 parts by weight of acrylamide and 6-12 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
S2, adding 0.05-1 part by weight of trimethylolpropane ethoxyl ester triacrylate into the obtained clear solution, stirring for 5min, standing until no bubble exists, adding 0.05-1 part by weight of initiator and 0.05-1 part by weight of light absorber, and stirring for 5-15 min again to obtain the 3D printing hydrogel ink.
And S3, adding the obtained 3D printing hydrogel ink into a photo-curing 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
S4, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Compared with the prior art, the invention has the following advantages:
(1) According to the invention, the plurality of tooth strips arranged on the oversleeve body are matched with the rotary threads encircling the tooth strips, so that the friction force between the vascular wall and the oversleeve support can be effectively increased, the blood vessel is not easy to fall off from the oversleeve support when being sutured, the blood vessel can be sutured rapidly and effectively, and the occurrence of unsmooth blood flow and even ischemic necrosis of the blood vessel due to eversion for a long time can be well prevented.
(2) According to the 3D printing hydrogel composed of the tridecyl methacrylate, the acrylic acid, the acrylamide, the absolute ethyl alcohol, the trimethylol propane ethoxy triacrylate and the initiator, the polymerization speed of the acrylic acid is high, the acrylic acid and the acrylamide can be polymerized to generate a homopolymer or a copolymer, the printing precision of the 3D printing oversleeve support can be effectively improved, and meanwhile, the tridecyl methacrylate of a hydrophobic monomer and the trifunctional cross-linking agent trimethylol propane ethoxy triacrylate are introduced for cross-linking, so that the precision and toughness of the oversleeve support are higher, and the size of the printed oversleeve support is more stable.
(3) According to the invention, through the regulation and control of the parts of the acrylic acid and the acrylamide and the addition of the light absorber, the penetration depth of ultraviolet light can be effectively reduced, so that the accuracy of the 3D printing oversleeve support is improved well.
(4) According to the invention, by setting the compatible I-type photoinitiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphinate as an initiator, and adjusting the purity of the initiator, the mechanical property of the oversleeve bracket for 3D printing is effectively ensured to be improved.
Drawings
Fig. 1 is a schematic diagram of the overall structure of the present invention.
The reference numerals in the drawings are: 1-sleeve body, 2-tooth strip, 3-rotation screw thread and 4-positioning clamping arm.
Detailed Description
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
Example 1
The 3D printing hydrogel oversleeve support for liver transplantation vascular anastomosis of the embodiment is shown in fig. 1, and the 3D printing hydrogel oversleeve support comprises a oversleeve body 1, tooth strips 2, rotary threads 3 and a positioning clamp arm 4. Specifically, the sleeve body 1 is a hollow body in this embodiment, and has two ends open, and is formed by photo-curing 3D printing. The number of the tooth strips 2 is a plurality of, the tooth strips 2 are uniformly distributed on the frame wall of the oversleeve body 1, the tooth strips 2 and the oversleeve body 1 are fixedly integrated, and the tooth strips are formed by photo-curing 3D printing. The tooth surface of the tooth strip 2 is arc-shaped so as to prevent the damage of the tooth surface of the tooth strip 2 to the vascular wall, and the safety and the reliability of the oversleeve bracket are well ensured. The rotary thread 3 surrounds the tooth bar 2, and the rotary thread 3 and the tooth bar 2 are fixed into a whole and are formed by photo-curing 3D printing. The locating clamp arm 4 is vertically fixed on the shaft mouth of the oversleeve body 1, and the locating clamp arm 4 and the oversleeve body 1 are fixed into a whole and are formed by photo-curing 3D printing. When the blood vessel suturing device is used, the toothed bar 2 and the rotary thread 3 are fully in close contact with the inner wall of the blood vessel, wherein friction force is generated between the toothed bar 2 and the inner wall of the blood vessel, the rotary thread 3 can better fix the blood vessel on the toothed bar 2, the blood vessel is not easy to fall off from the oversleeve support when suturing, the blood vessel can be sutured rapidly and effectively, and the blood vessel is prevented from blood flow unsmooth even ischemic necrosis of the blood vessel due to eversion for a long time. Simultaneously, when in use, the hemostatic forceps are clamped on the positioning clamping arms 4, so that the fixation of the oversleeve support can be realized, the oversleeve support is prevented from being shifted, and the blood vessel can be sutured better.
In addition, the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis consists of the following substances in parts by weight: 0.05 to 4 parts of tridecyl methacrylate, 1 to 4 parts of acrylic acid, 1 to 4 parts of acrylamide, 6 to 12 parts of absolute ethyl alcohol, 0.05 to 1 part of trimethylolpropane ethoxyester triacrylate, 0.05 to 1 part of initiator and 0.05 to 1 part of light absorber.
Wherein the density of the tridecyl methacrylate is 0.85g/cm 3 ～1g/cm 3 . The mass ratio of the acrylic acid to the acrylamide is 1:1; wherein the octanol/water distribution coefficient of the acrylic acid is 0.3-0.05, the viscosity (mPas, 25 ℃) is 1.256 to (mPas, 25 ℃) is 1.5, the relative density (25 ℃ and 4 ℃) is 1.024 to (25 ℃ and 4 ℃) is 1.4. The density of the acrylamide is 1.322g/cm 3 ～1.4g/cm 3 The melting point was 87 ℃. The light absorber is one or more of curcumin, fast green, lemon yellow and sudan.
In this example, 2 parts by weight of tridecyl methacrylate, 1 part by weight of acrylic acid, 1 part by weight of acrylamide, 6 parts by weight of absolute ethyl alcohol, 0.05 part by weight of trimethylolpropane ethoxyester triacrylate, 0.05 part by weight of initiator, and 0.05 part by weight of light absorber. Wherein the density of the tridecyl methacrylate is 0.85g/cm 3 . The mass ratio of the acrylic acid to the acrylamide is 1:1; wherein the octanol/water partition coefficient of the acrylic acid is 0.3, the viscosity (mPas, 25 ℃ C.) is 1.256, and the relative density (25 ℃ C., 4 ℃ C.) is 1.024. The initiator is a cell compatible I-type photoinitiator of lithium phenyl (2, 4, 6-trimethyl benzoyl) phosphinate, and the purity of the initiator is 99 percent. Acrylamide density of 1.322g/cm 3 The melting point was 87 ℃. The light absorbent is a mixture of curcumin, lemon yellow and Sudan.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 2 parts by weight of tridecyl methacrylate, 1 part by weight of acrylic acid, 1 part by weight of acrylamide and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, then 0.05 part by weight of initiator and 0.05 part by weight of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Example 2
This embodiment is substantially the same as embodiment 1, except that:
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis in the embodiment comprises the following substances in parts by weight: 1 part of tridecyl methacrylate, 3 parts of acrylamide, 6 parts of absolute ethyl alcohol, 0.05 part of trimethylolpropane ethoxyester triacrylate, 0.05 part of initiator and 0.05 part of light absorber.
Wherein the density of the tridecyl methacrylate is 1g/cm 3 . Acrylamide density of 1.4g/cm 3 The melting point was 87 ℃. The purity of the initiator was 99%. The light absorber is curcumin.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 1 part by weight of tridecyl methacrylate, 3 parts by weight of acrylamide and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, and then 0.05 part by weight of initiator and 0.05 part of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Example 3
This embodiment is substantially the same as embodiment 1, except that:
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis in the embodiment comprises the following substances in parts by weight: the weight part of the tridecyl methacrylate is 0.05 part, the weight part of the acrylamide is 3.5 parts, the weight part of the absolute ethyl alcohol is 6 parts, the weight part of the trimethylolpropane ethoxyester triacrylate is 0.05 part, the initiator is 0.05 part, and the light absorber is 0.05 part. Wherein the density of the tridecyl methacrylate is 1g/cm 3 . The purity of the initiator was 98.5%. Acrylamide density of 1.4g/cm 3 The melting point was 87 ℃. The light absorber is Sudan.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 0.05 part by weight of tridecyl methacrylate, 3.5 parts by weight of acrylamide and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, then 0.05 part by weight of initiator and 0.05 part by weight of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Example 4
This embodiment is substantially the same as embodiment 1, except that:
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis in the embodiment comprises the following substances in parts by weight: 3.5 parts of tridecyl methacrylate, 1 part of acrylamide, 6 parts of absolute ethyl alcohol, 0.05 part of trimethylolpropane ethoxyester triacrylate, 1 part of initiator and 1 part of light absorber. Wherein the density of the tridecyl methacrylate is 1g/cm 3 . Acrylamide density of 1.4g/cm 3 The melting point was 87 ℃. The purity of the initiator was 99%. The light absorber is a mixture of lemon yellow and sudan.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 3.5 parts by weight of tridecyl methacrylate, 1 part by weight of acrylamide and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, 1 part by weight of initiator and 1 part by weight of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Example 5
This embodiment is substantially the same as embodiment 1, except that:
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis in the embodiment comprises the following substances in parts by weight: the weight part of the tridecyl methacrylate is 4, the weight part of the absolute ethyl alcohol is 6, the weight part of the trimethylolpropane ethoxyester triacrylate is 0.05, the initiator is 0.05 and the light absorber is 0.05. Wherein the density of the tridecyl methacrylate is 1g/cm 3 . The purity of the initiator was 99%. The light absorber is lemon yellow.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 4 parts by weight of tridecyl methacrylate and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, then 0.05 part by weight of initiator and 0.05 part by weight of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
Example 6
This embodiment is substantially the same as embodiment 1, except that:
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis in the embodiment comprises the following substances in parts by weight: the weight parts of acrylamide, absolute ethyl alcohol, trimethylol propane ethoxy ester triacrylate, initiator, light absorber and light absorber are respectively 4 parts, 6 parts and 0.05 part, 1 part and 1 part respectively. Wherein the density of the acrylamide is 1.4g/cm 3 The melting point was 87 ℃. The purity of the initiator was 99%. The light absorber is a mixture of curcumin and sudan.
In addition, the preparation steps of the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis are as follows:
firstly, adding 4 parts by weight of acrylamide and 6 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution.
Then, 0.05 part by weight of trimethylolpropane ethoxyl ester triacrylate is added into the obtained clear solution, after stirring for 5min, the solution is kept stand until no bubble state, 1 part by weight of initiator and 1 part by weight of light absorber are added, and after stirring for 5-15 min again, the 3D printing hydrogel ink is obtained.
Secondly, adding the obtained 3D printing hydrogel ink into a photocuring 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours.
And finally, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
In order to illustrate the difference in accuracy and tensile strength between the oversleeve brackets prepared in examples 1 to 6 of the present invention and the existing oversleeve brackets, the oversleeve brackets prepared in examples 1 to 6 were respectively tested for tensile strength and printing accuracy. In the test, the length, the pipe diameter and the pipe wall thickness of the selected oversleeve bracket are consistent, and the test data are shown in the following table 1:
| test object | Tensile Strength (kPa) | Printing precision (mum) |
| Prior Art | 80 | 100 |
| Example 1 | 320 | 5 |
| Example 2 | 30 | 10 |
| Example 3 | 130 | 100 |
| Example 4 | 70 | 5 |
| Example 5 | 50 | 100 |
| Example 6 | 65 | 100 |
TABLE 1
From the above table 1, it can be well obtained that the tensile strength of the 3D printing hydrogel cuff support of each of examples 1 and 3 of the present application is better than that of the prior art cuff support, and the printing accuracy of the 3D printing hydrogel cuff supports of examples 1, 2 and 5 is better than that of the prior art cuff support. The tensile strength and the printing precision of the 3D printing hydrogel oversleeve bracket prepared in the embodiment 1 of the present invention are better than those of the 3D printing hydrogel oversleeve bracket prepared in other embodiments and the existing oversleeve bracket, and the components and the weight parts of the 3D printing hydrogel oversleeve bracket in the embodiment 1 of the present invention are optimal. In addition, the test result shows that the purity of the initiator can be adjusted by controlling the octanol/water distribution coefficient, viscosity and relative density of the acrylic acid and the components, density and melting point of the acrylamide, so that the tensile strength and printing precision of the 3D printing hydrogel oversleeve bracket can be changed.
Therefore, the 3D printing hydrogel composed of the tridecyl methacrylate, the acrylic acid, the acrylamide, the absolute ethyl alcohol, the trimethylol propane ethoxyl ester triacrylate, the initiator and the light absorber is described, the space distribution of light is regulated by taking the acrylic acid and the acrylamide as main components in a phase separation behavior in a photocuring process, meanwhile, the printing precision of the 3D printing oversleeve support can be effectively improved under the combined action of the acrylic acid, the acrylamide and the light absorber, and meanwhile, the precision and the toughness of the oversleeve support can be higher and the size of the printed oversleeve support is more stable through the cross-linking of the tridecyl methacrylate and the trifunctional cross-linking agent trimethylol propane ethoxyl ester triacrylate which are introduced with the hydrophobic monomer.
As described above, the present invention can be well implemented.
Claims (7)
1. The 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis is characterized by comprising a oversleeve body (1), a plurality of tooth strips (2) uniformly distributed on the frame wall of the oversleeve body (1), rotating threads (3) encircling the tooth strips (2) and positioning clamping arms (4) vertically fixed on the shaft mouth of the oversleeve body (1); the tooth surface of the tooth strip (2) is arc-shaped;
the 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis consists of the following substances in parts by weight: 0.05 to 4 parts of tridecyl methacrylate, 1 to 4 parts of acrylic acid, 1 to 4 parts of acrylamide, 6 to 12 parts of absolute ethyl alcohol, 0.05 to 1 part of trimethylolpropane ethoxyester triacrylate, 0.05 to 1 part of initiator and 0.05 to 1 part of light absorber.
2. The 3D printed hydrogel sleeve stent for liver graft vascular anastomosis of claim 1 wherein the density of tridecyl methacrylate is 0.85g/cm 3 ～1g/cm 3 。
3. The 3D printed hydrogel cuff stent for liver graft vascular anastomosis of claim 2, wherein the mass ratio of acrylic acid to acrylamide is 1:1; wherein the octanol/water distribution coefficient of the acrylic acid is 0.3-0.05, the viscosity (mPas, 25 ℃) is 1.256 to (mPas, 25 ℃) is 1.5, the relative density (25 ℃ and 4 ℃) is 1.024 to (25 ℃ and 4 ℃) is 1.4.
4. The 3D printed hydrogel cuff stent for liver graft vascular anastomosis of claim 3, wherein the acrylamide has a density of 1.322g/cm 3 ～1.4g/cm 3 The melting point was 87 ℃.
5. The 3D printed hydrogel cuff scaffold for liver graft vascular anastomosis according to claim 4, wherein the initiator is a cell compatible type I photoinitiator lithium phenyl (2, 4, 6-trimethylbenzoyl) phosphinate with a purity of not less than 98.5%.
6. The 3D printed hydrogel cuff stent for liver graft vascular anastomosis of claim 5, wherein the light absorber is one or a mixture of more of curcumin, fast green, lemon yellow and sudan.
7. The 3D printed hydrogel cuff stent for liver graft vascular anastomosis according to any one of claims 1 to 6, wherein the preparation steps of the 3D printed hydrogel cuff stent for liver graft vascular anastomosis are as follows:
s1, adding 0.05-4 parts by weight of tridecyl methacrylate, 1-4 parts by weight of acrylic acid, 1-4 parts by weight of acrylamide and 6-12 parts by weight of absolute ethyl alcohol into a vessel, stirring clockwise for 5-15 min, standing for 5min, and stirring anticlockwise for 5-15 min to obtain a clear solution;
s2, adding 0.05-1 part by weight of trimethylolpropane ethoxyl ester triacrylate into the obtained clear solution, stirring for 5min, standing until no bubble exists, adding 0.05-1 part by weight of initiator and 0.05-1 part by weight of light absorber, and stirring for 5-15 min again to obtain the 3D printing hydrogel ink;
s3, adding the obtained 3D printing hydrogel ink into a photo-curing 3D printing curing pool for printing to obtain a primary oversleeve bracket, and soaking the obtained primary oversleeve bracket in 5wt% ferric chloride hexahydrate ethanol solution at room temperature for reaction for 4 hours;
s4, taking out the soaked primary oversleeve support, repeatedly washing excessive ferric chloride with absolute ethyl alcohol, and naturally air-drying to obtain the final oversleeve support.
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| CN202310831432.6A CN117796870A (en) | 2023-07-07 | 2023-07-07 | 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310831432.6A CN117796870A (en) | 2023-07-07 | 2023-07-07 | 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis |
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| CN202310831432.6A Pending CN117796870A (en) | 2023-07-07 | 2023-07-07 | 3D printing hydrogel oversleeve bracket for liver transplantation vascular anastomosis |
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| CN (1) | CN117796870A (en) |
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