CN117778270A - Efficient DMF degrading strain and application of microbial inoculum thereof in process - Google Patents
Efficient DMF degrading strain and application of microbial inoculum thereof in process Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a strain for efficiently degrading DMF, which is Paracoccus ammoniaphaeovorusParacoccus aminovorans) The strain is named ZC2305-JX and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 12/11 of 2023, wherein the strain is provided with the preservation number: CGMCC No.29297. The invention provides a high-efficiency degradation strain capable of tolerating high-concentration DMF, and simultaneously provides a fermentation culture process of high-efficiency degradation bacteria of DMF, which realizes rapid enrichment and expansion culture of the strain in a short time, and develops a process for treating high-concentration DMF wastewater by taking the strain as a core so as to solve the problems of poor biodegradability of DMF, slow growth of degradation bacteria, difficult wastewater treatment and the like.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a strain for efficiently degrading DMF, a microbial inoculum thereof and application thereof in a process.
Background
N, N-Dimethylformamide (DMF) of the formula (CH) 3 ) 2 NHCHO is a colorless organic solvent with stable chemical property and excellent performance, and is widely used in pesticide, medicine, chemical industry and other industries because of being mutually soluble with water and most organic solvents except halogenated hydrocarbon, which is called as a universal solvent. DMF can enter human body through respiratory tract, alimentary canal and skin, has certain toxicity, and has higher liver function abnormality rate than common people according to investigation and people who are in long-term contact with DMF, and is accompanied with symptoms such as anorexia, abdominal distention and the like. The DMF is determined to be grade II (moderate hazard) by grading the hazard degree of occupational contact poison in China, and is an experimental animal carcinogen, and the recommended value of the highest allowable mass concentration in ground water in China is 25mg.L -1 . Since DMF is only used as an organic solvent and does not undergo chemical reaction, little loss is produced in quantity, and the DMF is completely introduced into production wastewater, if the DMF is not treated, the environment is greatly polluted.
At present, common treatment methods for DMF wastewater in industrial production include a physical chemical method, a chemical method and a biological method. But the cost of DMF wastewater treatment by a physical method and a chemical method is higher, the cost of DMF wastewater treatment by a biological method is lower, the operation management is simple, no secondary pollution is caused, and the microorganism has strong variability and domestication adaptability, is suitable for wastewater of various types and conditions, and is easy to be applied to actual wastewater treatment engineering on a large scale. Biological processes have therefore become an important study for DMF wastewater treatment. But DMF has stable chemical property, two methyl groups are arranged at the molecular end, and the B/C is 0.065, so that the DMF is difficult to biodegrade, and has an inhibition effect on the biological treatment process of wastewater, and the treatment effect is affected. Therefore, screening a strain which can endure high-concentration DMF and is highly effective is a key for improving the biological treatment of DMF wastewater, and the technology for treating high-concentration DMF wastewater is developed by taking the strain as a core, which has important significance.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a strain capable of tolerating high-concentration DMF and a fermentation culture process of DMF high-efficiency degradation bacteria, so that the strain is rapidly enriched and expanded in a short time, and a process for treating high-concentration DMF wastewater is developed by taking the strain as a core, so that the problems of poor biodegradability of DMF, slow growth of degradation bacteria, difficult wastewater treatment and the like are solved.
In order to achieve the above object, the present invention adopts the following technical scheme:
a strain capable of efficiently degrading DMF (dimethyl formamide) is Paracoccus aminophaeovoransParacoccus aminovorans) The strain is named ZC2305-JX and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 12/11 of 2023, wherein the strain is provided with the preservation number: CGMCC No.29297.
The application of the degradation strain in treating high-concentration DMF wastewater is that the concentration of DMF in the wastewater is 1-3 g/L, the temperature is 25-35 ℃, and the pH is 6-8.
A microbial inoculum prepared from degradation strain.
The preparation method of the microbial inoculum comprises the following specific steps:
s1, activating: single colony is selected from the solid plate and transferred into a liquid culture medium containing DMF pollutants, and the culture is carried out until the logarithmic phase;
s2, switching: transferring the bacterial liquid in the logarithmic phase into a seed tank for culture;
s3, expanding culture: transferring the seed liquid cultured in the seed tank into a fermentation tank for expansion culture to obtain the DMF high-efficiency degradation microbial inoculum.
Preferably, in the aforementioned step S1, the liquid medium comprises the following components: DMF 0.5g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, water balance; the culture temperature is 33-37 ℃ and the culture time is 8-16 h.
Preferably, in the aforementioned step S2, the medium composition of the seed tank is: DMF 2 g/L,NaCl 10g/L、KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, the balance being water; the inoculation amount of the bacterial liquid is 2-5% by volume.
Preferably, in the aforementioned step S3, the fermenter medium composition is the same as the seed tank medium composition; the inoculation amount of the seed liquid is 2-5% by volume; after fermentation, the effective viable count of the tank bacterial liquid is more than 10 9 cfu/ml。
The application of the microbial inoculum in the treatment of high-concentration DMF wastewater, wherein the concentration of DMF in the wastewater is 1-3 g/L, the temperature is 25-35 ℃, and the pH is 6-8.
A method for treating high-concentration DMF wastewater by using a microbial inoculum comprises the following specific steps: the microbial inoculum is put into the wastewater according to the inoculation amount of 3% -5% (V/V), and the wastewater is uniformly stirred for degradation.
The invention has the advantages that: the degradation strain of the invention can degrade DMF to convert dimethylamine and formate, and further degrade DMF to NH 3 And CO 2 The efficient degradation of DMF is realized, the degradation capacity is very strong, 99% biochemical degradation of DMF with initial concentration of 3g/L can be realized within 48h, and the treatment effect is very ideal; the strain can be continuously cultured and applied in DMF wastewater, has good DMF tolerance, can tolerate DMF about 5g/L at most, and has certain advantage effect when being impacted by the outside.
Drawings
FIG. 1 is a colony morphology of strain ZC 2305-JX;
FIG. 2 is a graph showing COD degradation curves of DMF at different concentrations;
FIG. 3 is a graph showing ammonia nitrogen release profiles for DMF at various concentrations;
FIG. 4 is a diagram showing the gas phase detection of the system before and after the treatment of the strain ZC 2305-JX;
FIG. 5 is a graph showing the effect of different temperatures on DMF degradation by strain ZC 2305-JX;
FIG. 6 is a graph showing the effect of different pH on DMF degradation by strain ZC 2305-JX;
FIG. 7 is a degradation curve diagram of the strain ZC2305-JX on the actual chemical wastewater;
FIG. 8 is a process flow diagram of the strain ZC2305-JX for treating high concentration DMF wastewater;
FIG. 9 is a graph showing the variation of COD and ammonia nitrogen concentration in effluent of each unit in example 7.
Description of the embodiments
The invention is described in detail below with reference to the drawings and the specific embodiments.
Example 1
Isolation and screening and identification of strain ZC 2305-JX:
(1) Domestication enrichment
50ml of activated sludge is taken and added into a 1L domestication device containing basic culture solution with DMF as the only carbon source and nitrogen source, the activated sludge is from a secondary sedimentation tank of a sewage plant for long-term treatment of DMF-containing wastewater in a chemical industry in the south of China, and the basic culture solution comprises the following formula: DMF 0.5g/L, naCl g/L, KH 2 PO4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L, the balance being water, pH 7.2-7.8. Culture temperature: and introducing oxygen to perform domestication culture for about 15-20 days at the temperature of 33-37 ℃, and periodically monitoring the concentration of COD and ammonia nitrogen and the growth condition of sludge in the screening process. And after detecting that DMF degradation is complete, changing water in time for supplementing, and gradually increasing the concentration of DMF to 5g/L to obtain enriched liquid of DMF pollutant degrading bacteria.
(2) Separation
Under aseptic conditions, transferring the domesticated enriched bacterial solution into an aseptic fresh basic culture solution which takes DMF as the sole carbon source and nitrogen source according to the inoculation amount of 2 percent, and carrying out shake culture at 35 ℃ until the basic culture solution reaches the logarithmic phase, wherein the formula of the basic culture solution is the same as that of the step (1). Strain isolation using the dilution-coated plate method: sucking 1mL of suspension containing DMF high-efficiency degradation strain, and diluting to 10 with sterile distilled water -1 ～10 -7 Gradient of 10 respectively -3 ～10 -7 The diluted bacterial liquid of (2) is coated on a DMF-containing inorganic salt solid culture medium, and the culture medium comprises the following components: DMF 1g/L, naCl 10g/L, agar powder 15 g/L, pH 7.2-7.8, and culturing in 35 deg.C incubator for 48 hr.
(3) Purification
In an ultra-clean workbench, picking single colonies growing in the DMF separation solid medium by using an inoculating needle, and further streaking, purifying and culturing the DMF degradation bacteria by using a plate streaking method until the single colonies grow. Repeating the operation for more than 3 times until the colony form growing on the plate is single, thus obtaining a strain of purified DMF pollutant high-efficiency degrading bacteria. A photograph of the plate streaked culture dish is shown in FIG. 1.
Sequencing the purified DMF pollutant degrading bacteria with the sequence shown as SEQ.NO.1 as the sequence of P.aminovoransParacoccus aminovorans) Named ZC2305-JX. The main physiological characteristics of the DMF degrading bacteria are as follows: the colony is round, is milky white, has the diameter of about 1-4 mm, is smooth and convex in surface, neat in edge, opaque, gram-negative in dyeing, and can grow aerobically by taking DMF as a sole carbon source and energy source.
The strain is preserved in China general microbiological culture Collection center (China Committee) for culture Collection of microorganisms at the year of 2023 and 12 and 11, and is classified and named as Paracoccus ammoniaphaeoidesParacoccus aminovorans) The laboratory was named ZC2305-JX with the deposit address: the Beijing city, the Chaoyang district, north Chenxi lu 1, 3, the strain preservation number is: CGMCC No.29297.
Example 2
Preparation of a microbial inoculum:
s1, activation: single colony of DMF pollutant high-efficiency degrading bacteria is selected from a solid plate and is transferred into a liquid culture medium containing DMF pollutant, wherein the culture medium comprises the following components: DMF 0.5g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, the balance of water, pH 7.2-7.8, and sterilizing at 121 ℃ under high pressure for 20 min; the culture system is 10mL, the rotation speed of a shaking table is 130~150rpm,33~37 ℃, and the shaking table is used for culturing for 8-16 hours until the logarithmic phase.
S2 switching: transferring the efficient degradation bacterial liquid of the DMF pollutants with S1 activation in the logarithmic phase into a 1L seed tank according to the access amount of 2% -5% for culturing, controlling the temperature of the seed tank to be maintained at 35+/-1 ℃ and the rotating speed to be maintained130-150 rpm, controlling dissolved oxygen DO at 4-6 mg/L, and culturing for 18-30 h, so that the strain can tolerate higher DMF concentration, and can obviously improve the complete degradation rate of DMF, and the culture medium components of the seed tank are as follows (mass ratio): DMF 2 g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, and the balance of water, and the pH is maintained between 7.5 and 8.0 by implementing a pH self-control adjustment technology.
S3, expanding culture: transferring the DMF pollutant high-efficiency degradation bacterial liquid cultured in the S2 seed tank into a 100L fermentation tank for expansion culture according to the inoculation amount of 2% -5%, wherein the culture medium components of the fermentation tank are the same as those of the seed tank, and the physical and chemical parameter indexes are as follows: the temperature is 33-37 ℃, the rotating speed is 130-150 rpm, the dissolved oxygen is 4-6 mg/L, and the fermentation time is 48-72 h; after fermentation, the effective viable count of the tank bacterial liquid can reach 10 9 And (3) after cfu/ml is exceeded, taking out the fermentation culture solution from the tank, and packaging the fermentation culture solution by a plastic barrel to obtain the DMF high-efficiency degradation microbial inoculum.
Example 3
Effect of DMF of different initial concentrations on degradation of COD and ammonia nitrogen by strain ZC 2305-JX:
the microbial inoculum obtained in example 2 was transferred to a shaking flask containing DMF at an inoculum size of 5% and an inorganic salt medium formulation comprising: naCl 10g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, water balance, pH 7.2-7.8. The added concentration of DMF in the inorganic salt culture medium is 1g/L, 3g/L, 5g/L and 5.5g/L respectively, 3 times of the culture are repeated, the rotation speed of a shaking table after inoculation is 180 rpm, the temperature is 35 ℃, and the culture is carried out in a shaking table. And sampling and measuring COD and ammonia nitrogen concentration in 0h, 12h, 24h, 36h and 48h respectively, wherein the COD is measured by a potassium dichromate method, the ammonia nitrogen concentration is measured by a Nashi reagent spectrophotometry method, and the measurement results are shown in figures 2 and 3.
As can be seen from FIG. 2, under the condition of DMF with different initial concentrations, the strain ZC2305-JX grows slowly in the hysteresis period, so that the strain degradation is also slower, and the degradation speed is obviously improved along with the increase of the concentration of thalli. The higher the initial concentration of DMF is, the longer the hysteresis period is, the supernatant is taken to pass through a 0.2um filter membrane to detect COD and ammonia nitrogen concentration, when the concentration of DMF is 1-2 g/L, the degradation rate of COD can reach 99% in 24 hours, when the concentration is 3g/L, the degradation rate of DMF can reach more than 99% in 48 hours, when the concentration is 5g/L, the degradation rate of DMF in 24 hours is 37%, the degradation rate of DMF can reach more than 85% in 48 hours, and in addition, the ammonia nitrogen concentration in the system gradually rises along with the degradation of DMF. As shown in FIG. 3, the ammonia nitrogen content at 48h reaches more than 99% of the theoretical ammonia nitrogen release value when the DMF concentration is 1-3 g/L, and the ammonia nitrogen content at 48h is only about 85% of the theoretical ammonia nitrogen release value when the DMF is 5-g/L, so that the strain ZC2305-JX has the best effect of degrading DMF when the initial DMF concentration is 1-3 g/L, and the strain is inhibited and the degradation capability of DMF is reduced when the concentration is more than 5 g/L.
And detecting the experimental group with the DMF addition amount of 3g/L by adopting gas chromatography, sampling and centrifuging a system before and after the DMF degradation bacteria are treated for 48 hours, and taking supernatant for detection, wherein the detection result is shown in figure 4.
As can be seen from FIG. 4, DMF is metabolized and degraded to NH 3 And CO 2 Degradation and removal of DMF are realized. The data show that the strain ZC2305-JX has good tolerance and degradation effect on DMF.
Example 4
Effect of different temperatures on DMF degradation effect of strain ZC 2305-JX:
the microbial inoculum obtained in example 2 was transferred to a shaking flask containing DMF at an inoculum size of 5% and an inorganic salt medium formulation comprising: DMF 3g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, balance water, pH 7.5. The shaking table rotation speed after inoculation is 180 rpm, the temperature is 20 ℃, 25 ℃, 30 ℃,35 ℃, 40 ℃ and 45 ℃ respectively, the continuous shake culture is carried out for 48 hours, and then the DMF concentration is sampled and measured, and the measurement result is shown in figure 5.
As shown in FIG. 5, the temperature has a significant effect on DMF degradation effect of the strain ZC2305-JX, the DMF removal rate is gradually increased when the temperature is increased from 20 ℃ to 35 ℃, the DMF removal effect is optimal when the temperature is 35 ℃, the DMF removal rate is more than 99%, the optimal degradation temperature is 25-35 ℃, and the DMF removal rate is obviously reduced when the temperature is continuously increased, so that the strain is inactivated by the high temperature, and the degradation effect is affected.
Example 5
Effect of different pH on DMF degradation effect of strain ZC 2305-JX:
the microbial inoculum obtained in example 2 was transferred to a shaking flask containing DMF at an inoculum size of 5% and an inorganic salt medium formulation comprising: DMF 3g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, water balance, temperature 35 ℃. The shaking table rotational speed after inoculation was 180 rpm, and the pH was 5, 6, 7, 8, 9, 10, respectively, and after 48 hours of continuous shake culture, the DMF concentration was measured by sampling, and the measurement results are shown in FIG. 6.
As shown in FIG. 6, when the pH is 6-8, the strain ZC2305-JX has better degradation effect on DMF, when the pH is 8, the degradation effect is optimal, the removal rate is more than 99%, and when the pH is lower than 6 or higher than 8, the strain ZC2305-JX has obviously reduced degradation effect on DMF, because the normal physiological activities of microorganisms are inhibited when the pH is too low, the thalli are damaged, and the degradation effect is reduced; when the pH is too high, free ammonia in the system increases, resulting in inhibition of biological activity and affecting degradation effect.
Example 6
Application of strain ZC2305-JX in actual wastewater:
in order to verify the application effect of DMF pollutant high-efficiency degrading bacteria ZC2305-JX in actual DMF-containing wastewater, DMF-containing production wastewater is obtained from a sewage station regulating tank of a certain chemical enterprise. The measurement shows that the DMF concentration in the wastewater is about 1.5g/L, the pH of the raw sewage is 6.0-8.0, the COD is about 3.1 g/L, the ammonia nitrogen is about 0.13 g/L, and the total phosphorus is 0.01g/L. The DMF pollutant degrading bacteria ZC2305-JX fermentation liquor is directly added into the production wastewater of DMF according to the inoculum size of 5% (V/V), and the pH value of the system is regulated to be about 7.5 so as to meet the requirement of the normal growth of microorganisms. Under the condition of room temperature aeration, the change of DMF concentration in the system is monitored by sampling, and DMF degradation data is shown in figure 7.
As shown in FIG. 7, DMF in the wastewater is degraded within 12h, the concentration of DMF is reduced to 14 mg/L at 24h, and the degradation rate is as high as 99%.
Example 7
The high-concentration DMF wastewater treatment process with the strain ZC2305-JX as a core is practically applied to:
the wastewater containing high-concentration DMF in an actual sewage plant is introduced into a degradation pool containing DMF high-efficiency degradation bacteria (3% -5% of inoculum size), and the high-concentration DMF can be rapidly degraded into NH 3 CO and CO 2 The DMF high-efficiency degrading bacteria discharged along with the effluent can be refluxed to the DMF degrading tank through the cyclone separator, the reaction tank is maintained to contain high-concentration DMF high-efficiency degrading bacteria, the upper liquid after cyclone enters the subsequent short-cut nitrification tank, and NH is obtained 3 Conversion to NO 2 - -N, finally NO by denitrification 2 - Conversion of-N to N 2 The process can realize the rapid degradation and removal of the high-concentration DMF, can be used as a main flow or side flow process to be integrated into a sewage plant system, and the specific process flow is shown in figure 8.
In order to further verify the application effect of degrading bacteria ZC2305-JX in actual DMF-containing wastewater, the industrial wastewater containing high concentration DMF is obtained from a sewage station regulating tank of a certain chemical enterprise. The measurement shows that the concentration of DMF in the wastewater is 2.31g/L, the concentration is higher, the pH of the raw sewage is 7.0-8.0, the COD is about 3.8 g/L, and the ammonia nitrogen is about 0.2 g/L. The DMF pollutant degrading bacteria ZC2305-JX fermentation liquor is directly added into a DMF degradation tank according to the inoculation amount of 10% (V/V), and the pH value of the system is regulated to be stabilized at about 7.5 so as to meet the requirement of the normal growth of microorganisms. The effluent of the degradation tank is separated by a hydrocyclone and then enters a short-range nitrification tank, ammonia nitrogen can be converted into nitrite nitrogen under the condition of room temperature aeration, and then the nitrite nitrogen is removed by the denitrification tank. The hydraulic retention time is as follows: the DMF degradation tank 24h, the short-cut nitrification tank 12h and the denitrification tank 6h are used for sampling effluent samples of each unit at regular time, monitoring the change of COD and ammonia nitrogen concentration in effluent of each unit, and the data are shown in figure 9.
As shown in FIG. 9, the COD concentration of the inlet water is about 3.8 g/L, the ammonia nitrogen concentration is about 0.2 g/L, the COD of the effluent of the DMF degradation tank is about 482 mg/L, the COD removal rate is more than 87%, the COD of the effluent after passing through the denitrification tank is only 46 mg/L, the effluent meets the first-stage A emission standard, and the ammonia nitrogen concentration is less than 10mg/L at the moment, which indicates that the DMF is completely degraded. The high-concentration DMF wastewater treatment process taking the strain ZC2305-JX as a core has good treatment effect in actual DMF-containing wastewater and has wide application prospect.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be appreciated by persons skilled in the art that the above embodiments are not intended to limit the invention in any way, and that all technical solutions obtained by means of equivalent substitutions or equivalent transformations fall within the scope of the invention.
Claims (9)
1. A strain for efficiently degrading DMF is characterized in that the strain is Paracoccus aminophaeovoransParacoccus aminovorans) The strain is named ZC2305-JX and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 12/11 of 2023, wherein the strain is provided with the preservation number: CGMCC No.29297.
2. Use of the strain according to claim 1 for treating high concentration DMF waste water, wherein the concentration of DMF in the waste water is 1-3 g/L, the temperature is 25-35 ℃, and the pH is 6-8.
3. A microbial agent prepared using the degradation strain of claim 1.
4. A method for preparing the microbial inoculum of claim 3, comprising the specific steps of:
s1, activating: single colony is selected from the solid plate and transferred into a liquid culture medium containing DMF pollutants, and the culture is carried out until the logarithmic phase;
s2, switching: transferring the bacterial liquid in the logarithmic phase into a seed tank for culture;
s3, expanding culture: transferring the seed liquid cultured in the seed tank into a fermentation tank for expansion culture to obtain the DMF high-efficiency degradation microbial inoculum.
5. The method according to claim 4, wherein in the step S1, the liquid medium comprises the following components: DMF 0.5g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, and the balance of water.
6. The method according to claim 4, wherein in the step S2, the medium composition of the seed tank is: DMF 2 g/L, naCl g/L, KH 2 PO 4 0.1g/L、Na 2 HPO 4 0.1g/L、MgCl 2 0.02g/L、CaCl 2 0.03g/L、NaHCO 3 0.5g/L、Na 2 CO 3 0.5g/L, and the balance of water.
7. The method according to claim 7, wherein in the step S3, the composition of the medium in the fermenter is the same as that in the seed tank; after fermentation, the effective viable count of the tank bacterial liquid is more than 10 9 cfu/ml。
8. The use of the microbial inoculum of claim 3 in the treatment of high-concentration DMF wastewater, wherein the concentration of DMF in the wastewater is 1-3 g/L, the temperature is 25-35 ℃, and the pH is 6-8.
9. A method for treating high-concentration DMF waste water by using the microbial inoculum of claim 3, which is characterized by comprising the following specific steps: the microbial inoculum is put into the wastewater according to the inoculation amount of 3% -5% (V/V), and the wastewater is uniformly stirred for degradation.
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