CN117778194A - Coriolus strain and application thereof - Google Patents
Coriolus strain and application thereof Download PDFInfo
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- CN117778194A CN117778194A CN202310671797.7A CN202310671797A CN117778194A CN 117778194 A CN117778194 A CN 117778194A CN 202310671797 A CN202310671797 A CN 202310671797A CN 117778194 A CN117778194 A CN 117778194A
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- trametes versicolor
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- versicolor
- coriolus
- trametes
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- 241000222356 Coriolus Species 0.000 title abstract description 9
- 241000222355 Trametes versicolor Species 0.000 claims abstract description 81
- 238000004321 preservation Methods 0.000 claims abstract description 11
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 7
- 241000233866 Fungi Species 0.000 claims description 5
- 108700022487 rRNA Genes Proteins 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000002246 antineoplastic agent Substances 0.000 claims description 2
- 229940041181 antineoplastic drug Drugs 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 abstract description 19
- 229920001282 polysaccharide Polymers 0.000 abstract description 19
- 239000005017 polysaccharide Substances 0.000 abstract description 19
- 239000000835 fiber Substances 0.000 abstract description 6
- 235000019750 Crude protein Nutrition 0.000 abstract description 4
- 239000002023 wood Substances 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000004753 textile Substances 0.000 description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 2
- 229940107698 malachite green Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- CQPFMGBJSMSXLP-UHFFFAOYSA-M acid orange 7 Chemical compound [Na+].OC1=CC=C2C=CC=CC2=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 CQPFMGBJSMSXLP-UHFFFAOYSA-M 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
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- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000010919 dye waste Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical class O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
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- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
Abstract
The invention provides a Coriolus strain and application thereof, the Coriolus strain is named as Coriolus strain (Trametes versicolor) Yun Cheng No.1, the preservation number is CGMCC No.40382, the preservation date is 2023 years 1 month 16 days, and the preservation unit is China general microbiological culture Collection center. The trametes versicolor (Trametes versicolor) Yun Cheng number 1 is separated from southeast tannin-rotting wood, and the strain has strong environmental adaptability and is rich in crude polysaccharide, crude protein, crude fiber and other nutritional ingredients.
Description
Technical Field
The invention relates to the technical field of edible fungus preparation, in particular to a trametes versicolor strain and application thereof.
Background
Coriolus versicolor [ Coriolus uersicolor (L.: fr.) Quel. ] recorded in Ben Cao gang mu (volume 28), has the effects of strengthening body resistance and consolidating constitution and invigorating essence and qi. The Chinese pharmacopoeia records that corious versicolor has the effects of strengthening spleen, promoting diuresis, clearing heat and detoxicating, and is mainly used for treating jaundice due to damp-heat, hypochondriac pain, anorexia, fatigue, hypodynamia and the like. Modern medicine shows that coriolus versicolor has various effects of regulating immunity, resisting tumor, resisting atherosclerosis, etc.
The wild coriolus versicolor grows on broad-leaf sapwood or dried branches, is widely distributed in China, and is distributed in 21 provinces and 4 autonomous areas of Heilongjiang, hebei, shanxi, shandong, jiangsu, fujian, sichuan, yunnan and the like. The coriolus versicolor medicinal materials in China mainly come from wild collection, but the wild collection coriolus versicolor quality is unstable and high in price, the ecological environment is greatly destroyed, the yield is gradually reduced, and the yield and the quality can not meet the market demand. The polysaccharide content of most wild coriolus versicolor is obviously lower than that of coriolus versicolor defined in pharmacopoeia standards (pharmacopoeia of the people's republic of China).
At present, there have been many studies on trametes versicolor, such as: the invention patent with publication number of CN111961595A discloses a coriolus versicolor strain for efficiently decoloring various textile dyes, which is inoculated into a culture medium respectively containing 100mg/L Congo red, acid orange G, malachite green, coomassie brilliant blue R250, 10B, RB brilliant blue amino black and 50mg/L crystal violet, and the decoloring rate can respectively reach 99.34%, 58.24%, 99.54%, 99.53%, 82.46%, 89.08% and 83.55%. The strain coriolus versicolor has better decolorization effect on various textile dyes including congo red, malachite green and coomassie brilliant blue R250 than that of most strains at home and abroad, and the decolorization dye in the strain is easy to operate and is suitable for industrial treatment of textile dye waste liquid.
The invention patent with the publication number of CN109845573B discloses a new Coriolus strain which is deposited by China general microbiological culture Collection center, with the accession number: coriolus versicolor trametes versicolor Trametes Versicolor with CGMCC No. 15880. The invention also discloses a method for cultivating Coriolus versicolor. Compared with the data obtained after the cultivation and planting of the wild Coriolus versicolor strain, the production period of Coriolus versicolor No.1 is shortened by 13 days, the yield reaches 75.32g/kg of bag material, the yield is increased by more than 15.37 percent compared with the control strain, the extract and polysaccharide contents are respectively increased by 5.85 percent and 10.43 percent compared with the control strain, and the industrial production application prospect is good.
The polysaccharide content of the extracts of the strains involved in the above studies is still to be further increased. It is necessary to find new polysaccharide-enriched trametes versicolor, further increasing the polysaccharide content of the extract.
Disclosure of Invention
The invention provides a coriolus versicolor strain and application thereof, wherein the coriolus versicolor strain is rich in polysaccharide, and can effectively improve the content of polysaccharide in fruiting body extracts, thereby improving the drug property of drugs; can also improve the protein and fiber content of the product and reduce the fat content of the product.
The specific technical scheme is as follows:
the invention provides a Coriolus strain named Coriolus strain (Trametes versicolor) Yun Cheng No.1, the preservation number is CGMCC No.40382, the preservation date is 2023, 1 month and 16 days, and the preservation unit is China general microbiological culture Collection center.
Further, the sequence of the rRNA gene ITS1-5.8S-ITS2 region of the coriolus versicolor (Trametes versicolor) Yun Cheng No.1 is shown as SEQ ID NO. 1.
The invention also provides a solid fermentation product which is obtained by solid fermentation of the trametes versicolor strain; the trametes versicolor strain is described above.
The invention also provides solid fermentation fungus powder, which is characterized by being obtained by drying after solid fermentation of trametes versicolor strains; the trametes versicolor strain is described above.
The invention also provides application of the coriolus versicolor trametes versicolor strain in preparing antitumor drugs or health products.
Compared with the prior art, the invention has the following beneficial effects:
the trametes versicolor (Trametes versicolor) Yun Cheng number 1 is separated from southeast tannin-rotting wood, and the strain has strong environmental adaptability and is rich in crude polysaccharide, crude protein, crude fiber and other nutritional ingredients.
Drawings
FIG. 1 is a diagram showing the observation of mycelium of trametes versicolor (Trametes versicolor) Yun Cheng in a test tube.
FIG. 2 is a diagram showing the observation of mycelia of trametes versicolor (Trametes versicolor) Yun Cheng No.1 during bag cultivation.
Detailed Description
The invention will be further described with reference to the following examples, which are given by way of illustration only, but the scope of the invention is not limited thereto.
EXAMPLE 1 isolation and identification of trametes versicolor (Trametes versicolor) Yun Cheng No.1
The trametes versicolor (Trametes versicolor) Yun Cheng is obtained from southeast tannin sapwood by tissue separation, domestication and cultivation. The domestication cultivation and cultivation experiments show that the Yun Cheng No.1 genetic character is stable, and the fruiting body of the fruiting body meets the standard of the pharmacopoeia of the people's republic of China.
The tissue separation method comprises the following steps: on an ultra-clean workbench, taking tissue blocks of wild coriolus versicolor fruiting bodies on southeast tannin-wood, connecting the tissue blocks to a PDA inclined plane, placing the tissue blocks in a constant-temperature incubator at 25 ℃ for light-shielding cultivation for 5d, after hyphae germinate, picking the front ends of the hyphae, inoculating the front ends to a new PDA flat plate for purification cultivation (at 25 ℃ for 5 d) until pure hyphae are obtained, and then respectively transferring the tissue blocks to the PDA inclined plane and the flat plate for cultivation, and then preserving the tissue blocks at 4 ℃ for later use.
The identification by culture, microscopic morphological observation and ITS sequencing is as follows: coriolus versicolor Trametes versicolor (l.) Lloyd, which has the culture characteristics and morphology as follows: the growth is rapid on PDA culture medium, the colony diameter reaches 5.5 cm after culturing for 5 days at 25 ℃; culturing for 7 days, wherein the colony is round, the edge is irregular, white, velvet-shaped, compact in hypha, sparse in hypha at the edge, radial outwards and colorless at the back; culturing for 10 days, flatly attaching the colony, compacting hypha, white, flocculent and colorless at the back. The mycelium was colorless, smooth, branched, with septum, 2-4.5 μm wide, as shown in FIG. 1.
The sequence of the rRNA gene ITS1-5.8S-ITS2 region of the trametes versicolor (Trametes versicolor) Yun Cheng is shown in SEQ ID NO. 1.
EXAMPLE 2 preservation of trametes versicolor (Trametes versicolor) No. Yun Cheng 1
The rRNA gene ITS1-5.8S-ITS2 region sequence was subjected to blast comparison in NCBI database, and the result shows that the strain has a common origin with Trametes versicolor, so that the strain is identified as trametes versicolor (Trametes versicolor) and named as trametes versicolor (Trametes versicolor) Yun Cheng No. 1.
The strain is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.40382 in 2023 and 1-16 days.
EXAMPLE 3 solid fermentation and fruiting body cultivation of trametes versicolor (Trametes versicolor) Yun Cheng No.1
Preparing a fungus bag in 12 months, wherein the culture medium comprises 78% of wood dust, 20% of bran, 2% of gypsum and 55% of water in percentage by mass; bagging and sleeving the culture medium, sterilizing in a sterilizing pot at 128 ℃ for 1.5 hours, cooling to normal temperature (below 25 ℃), inoculating coriolus versicolor (Trametes versicolor) Yun Cheng # 1 to the bagged and sterilized culture medium, sterilizing at 25-27 ℃ at 15 g/bag (as shown in figure 2); and (3) after 35 days of fermentation, obtaining a solid fermentation product, drying, and grinding into powder to obtain the solid fermentation bacteria powder.
After 35 days of spawn running, hyphae are thoroughly grown, and the normal temperature culture can be continued for 20 days; and in the last ten days of 5 months, the fungus bags are moved under a shadow mask with the canopy density of 70 percent to be cultivated until fruiting, and fruiting bodies of trametes versicolor (Trametes versicolor) are obtained.
EXAMPLE 4 determination of polysaccharide content of trametes versicolor (Trametes versicolor) Yun Cheng No.1 solid fermentation powder
The crude polysaccharide content in the solid fermentation powder prepared in example 3 of coriolus versicolor (Trametes versicolor) Yun Cheng No.1 was determined by phenol sulfuric acid method, the polysaccharide was first hydrolyzed to monosaccharides by sulfuric acid, and rapidly dehydrated to give a furfural derivative, which was then reacted with phenol to give an orange-yellow compound, which was measured at 490nm wavelength using an ultraviolet spectrophotometer.
Using glucose as a standard, a standard curve between absorbance and polysaccharide concentration (y=8.905x+0.0471, y being absorbance, x being polysaccharide concentration) is established, R 2 =0.991, the conversion relationship between the two can be fitted well. Because the sample is powdery solid, the ultrasonic water bath method is adopted for preliminary wall breaking to obtain crude polysaccharide solution.
The specific parameters are as follows: ultrasonic power 150w, ultrasonic time 1h, ultrasonic temperature 50 ℃, and feed liquid ratio 1:100, after wall breaking is completed, placing the mixture in a centrifugal machine for centrifugation at 8000rpm for 20min, and discarding filter residues.
Results: polysaccharide concentration of the solid fermentation powder of trametes versicolor (Trametes versicolor) Yun Cheng is 105.985 mg/g+/-0.013 mg/g.
EXAMPLE 5 determination of nutrient content of fruiting body of trametes versicolor (Trametes versicolor) Yun Cheng 1
The fruiting body of trametes versicolor (Trametes versicolor) obtained in the bag cultivation of example 3 was subjected to determination of main nutrition components; wherein crude protein is measured according to Dummissik azotometer combustion method in national standard GB 5009.5-2010, crude fiber is measured according to national standard GB/T5009.10-2003, crude fat is measured according to Soxhlet extraction method in national standard GB 5009.5-2016, and polysaccharide is measured by the same method as described in example 4.
Results: the content of crude polysaccharide in fruiting body of Coriolus versicolor (Trametes versicolor) is (8.57+ -0.27)%; the crude protein content is (18.75+/-0.12)%; the crude fiber content is (25.75+/-0.52)%; the crude fat content was (1.37.+ -. 0.14)%. Therefore, the coriolus versicolor trametes versicolor (Trametes versicolor) Yun Cheng No.1 provided by the invention has the characteristics of high crude polysaccharide, high protein, high fiber and low fat.
Claims (5)
1. A strain of trametes versicolor is characterized in that the strain is named as trametes versicolor (Trametes versicolor) Yun Cheng, the preservation number is CGMCC No.40382, the preservation date is 2023, 1 month and 16 days, and the preservation unit is China general microbiological culture Collection center.
2. The trametes versicolor strain of claim 1, wherein the sequence of regions ITS1-5.8S-ITS2 of rRNA gene No. Yun Cheng of trametes versicolor (Trametes versicolor) is shown in SEQ ID NO. 1.
3. A solid fermentation product is characterized in that the product is obtained by solid fermentation of a trametes versicolor strain; the trametes versicolor strain is as claimed in claim 1.
4. The solid fermentation fungus powder is characterized by being obtained by solid fermentation of a trametes versicolor strain and drying; the trametes versicolor strain is as claimed in claim 1.
5. The use of the trametes versicolor strain according to claim 1 for the preparation of an antitumor drug or a health product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310671797.7A CN117778194A (en) | 2023-06-08 | 2023-06-08 | Coriolus strain and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310671797.7A CN117778194A (en) | 2023-06-08 | 2023-06-08 | Coriolus strain and application thereof |
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| Publication Number | Publication Date |
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| CN117778194A true CN117778194A (en) | 2024-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310671797.7A Pending CN117778194A (en) | 2023-06-08 | 2023-06-08 | Coriolus strain and application thereof |
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| CN (1) | CN117778194A (en) |
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2023
- 2023-06-08 CN CN202310671797.7A patent/CN117778194A/en active Pending
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