CN117755635A - Kit for detecting lung cancer related gene mutation and application thereof - Google Patents

Abstract

The invention relates to the field of biochemistry, and provides a kit for detecting lung cancer related gene mutation and application thereof, wherein the kit comprises: the cylinder body is connected with the bottom end of the cylinder body, a circular groove is formed in the bottom end of the cylinder body, a plurality of first arc grooves are formed in the cylinder body, a second arc groove is formed in the bottom end of the cylinder body, a plurality of L-shaped pipes are arranged in the cylinder body, one ends of the L-shaped pipes are communicated with one side of the arc grooves, a long hole is formed in the cylinder body, and a bouncing component is arranged in the long hole and positioned in the cylinder body; according to the invention, through reasonable collocation of the L-shaped tube, the pricking pin, the cover and the arc groove I, the solution in the L-shaped tube flows into the arc groove I, so that the situation that the length of the reagent needle needs to be correspondingly increased when the reagent groove is deeper, the insertion stroke of the reagent needle is larger and the like can be caused, and the taking of a disinfection paper towel by a staff is facilitated through reasonable collocation of the bouncing component.

Description

Kit for detecting lung cancer related gene mutation and application thereof

Technical Field

The invention relates to the field of biochemistry, in particular to a kit for detecting lung cancer related gene mutation and application thereof.

Background

The tumor is a new organism formed by abnormal proliferation of cells in a certain or some parts of tissues in the human body under the action of various adverse factors, and the abnormal proliferation of cells is tumor cells.

Primary lung cancer is the first of various malignant tumors with high incidence rate and death rate in China, and seriously threatens human health; about 80% -85% of lung cancer patients with global lung cancer incidence and mortality are non-small cell lung cancer (NSCL), about 50% of patients have advanced diagnosis and poor prognosis; the biological information of the tumor is accurately known, so that the personalized treatment under the genotyping guidance is important for guiding clinical medication.

In the field of biochemistry, it is often necessary to use a plurality of different reagents for detecting a biological sample, and an operator needs to prepare various reagents, reagent tubes and other auxiliary tools in advance before detecting the biological sample. The kit is provided with a plurality of independent reagent tanks, so that a plurality of required reagents can be placed in a concentrated mode, and an operator can absorb the required reagents only by inserting reagent needles into the corresponding reagent tanks, thereby facilitating detection.

However, the reagent needle needs to be inserted into the bottom of the reagent tank to maximize the use of the reagent in the reagent tank, so that when the reagent tank is deep, the length of the reagent needle needs to be increased correspondingly, and the insertion stroke of the reagent needle is large; and after the reagent needle is inserted into the bottom of the reagent tank, the vast area of the outer wall of the reagent needle can be soaked in the reagent, and before the next different type of reagent is required to be sucked, all the reagents attached to the outside of the reagent needle are required to be cleaned, so that the use amount of cleaning liquid and the cleaning difficulty are increased to a certain extent.

In addition, the reagent needle can extend into the next reagent tank after extending into the next reagent tank and the surface water is required to be wiped off, however, in the process, the sterilized paper towel can be placed on a table top, so that the sterilized paper towel is polluted by bacteria, and the detection result is affected; or more sterilized paper towels can be taken out, so that the sterilized paper towels are wasted; the taking of the sterilized paper towel is less, and the normal detection is interfered.

In summary, the invention provides a kit for detecting lung cancer related gene mutation and application thereof.

Disclosure of Invention

In order to solve the technical problems, the invention provides a kit for detecting the mutation of a lung cancer related gene and application thereof, and aims to solve the problems that the length of a reagent needle needs to be correspondingly increased when a reagent groove is deeper in the prior art.

The prior art scheme of the invention is as follows:

a kit for detecting a lung cancer-associated gene mutation, comprising: the cylinder, the cylinder bottom is connected with the cylinder, the cylinder bottom is provided with the circular slot, be equipped with a plurality of arc wall first in the cylinder, the bottom is provided with arc wall second under the cylinder, be equipped with a plurality of L type pipe in the cylinder, L type pipe one end and arc wall one side intercommunication, be equipped with rectangular hole on the cylinder, just be located rectangular hole in the cylinder and be equipped with the spring subassembly.

Further, one end of the L-shaped tube far away from the first arc-shaped groove is provided with a sealing film, one end of the L-shaped tube provided with the sealing film is in threaded connection with a cover, one end of the cover far away from the L-shaped tube is provided with a puncture needle, and the puncture needle is provided with a protective sleeve.

Further, the bouncing component comprises a fixing piece I and a panel; the bottom of the first fixing piece is fixedly connected to the circular groove, the top end of the first fixing piece is fixedly connected with a first poking piece, a rotating block is arranged between the two first poking pieces, and one end of the rotating block is connected with a ratchet wheel;

the panel bottom fixedly connected with spliced pole, the cover is equipped with the mounting three on the spliced pole, the spliced pole bottom is connected with the connecting rod, the cover is equipped with mounting four and mounting five on the connecting rod, be located and be provided with the spring between mounting four and the mounting five, connecting rod bottom fixedly connected with mounting two, mounting two rotates and is connected with plectrum two.

Further, a round hole is formed in the panel, a movable column is arranged in the round hole, and a limiting block is arranged at one end, close to the panel, of the movable column.

Further, a first long groove is formed in the periphery of the cylinder, and a second long groove is formed in the upper end face of the cylinder.

Further, the number of the arc-shaped grooves I, the arc-shaped grooves II, the L-shaped pipes and the long grooves I is the same, and the positions of the arc-shaped grooves I, the arc-shaped grooves II and the long grooves I are correspondingly on the same straight line.

Further, the cylinder body is a round table, and the radius of the round groove is consistent with the radius of the lower end surface of the cylinder body.

Compared with the prior art, the invention has the following beneficial effects:

1. according to the invention, through reasonable collocation of the L-shaped tube, the puncture needle, the cover and the arc groove I, the solution in the L-shaped tube flows into the arc groove I, so that the situation that the length of the reagent needle needs to be correspondingly increased when the reagent groove is deeper, the insertion stroke of the reagent needle is larger and the like can be avoided.

2. According to the invention, through reasonable collocation of the bouncing component, namely when the paper towel is pressed downwards, the panel drives the connecting column to downwards move to compress the spring, and simultaneously drives the connecting rod and the pulling piece to downwards move, and the pulling piece downwards moves to simultaneously drive the ratchet wheel to overturn, in the process of overturning the ratchet wheel, the protruding position on the ratchet wheel is contacted with the movable column, so that the movable column upwards moves and simultaneously drives the sterilized paper towel on the panel to upwards move, and the taking of the sterilized paper towel by a worker is facilitated.

Drawings

FIG. 1 is a schematic view of the overall front perspective structure of the present invention;

FIG. 2 is a schematic perspective view of the overall structure of the present invention;

FIG. 3 is a schematic top perspective view of the overall structure of the present invention;

FIG. 4 is a schematic perspective view of a bouncing assembly according to the present invention;

FIG. 5 is a schematic view showing the internal perspective structure of the L-shaped tube of the present invention.

In the figure:

1. a cylinder; 2. a cylinder; 3. an arc-shaped groove I; 4. an L-shaped tube; 5. an elongated hole; 6. a bouncing component; 60. a first fixing piece; 61. a first poking piece; 62. a rotating block; 63. a ratchet wheel; 64. a panel; 65. a connecting column; 66. a connecting rod; 67. a second fixing piece; 68. a second poking piece; 69. a third fixing piece; 610. a fifth fixing member; 611. a fifth fixing member; 612. a spring; 613. a round hole; 614. a movable column; 65. a limiting block; 7. an arc-shaped groove II; 8. a circular groove; 9. a first long groove; 10. a second long groove; 11. a cover; 12. a needle; 13. a cover plate II; 14. and (3) sealing the film.

Detailed Description

Embodiments of the present invention are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the invention but are not intended to limit the scope of the invention.

The invention provides a kit for detecting lung cancer related gene mutation, which comprises the following components: the utility model provides a paper sterilizing device, including barrel 1, barrel 1 bottom is connected with cylinder 2, cylinder 2 bottom is provided with circular slot 8, be equipped with a plurality of arc wall first 3 in the cylinder 2, the bottom is provided with arc wall second 7 under the barrel 1, place the test paper with L type pipe 4 corresponding solution in the arc wall second 7, and arc wall second 7 surface hinge connection has apron first (not shown in the picture), avoid the test paper to be polluted in the non-use, thereby extravagant material or make experimental data inaccurate, be equipped with a plurality of L type pipe 4 in the barrel 1, L type pipe 4 internal storage detects solution, can be EGFR solution, HER2 solution, DNA polymerase solution etc. detects solution, L type pipe 4 one end and arc wall first 3 side intercommunication, seal film on L type pipe 4 is pricked, detect solution can be left to in the arc wall first 3 from L type pipe 4, and the degree of depth capacity of arc wall first 3 is greater than L type pipe 4 internal storage detects solution's capacity, and the degree of depth of arc wall first 3 is shallower, prevent to influence the experiment result because of probe length is inaccurate, be equipped with rectangular 5 on the barrel 1, be equipped with on the barrel 5, be equipped with paper towel 5, it is connected with paper towel 5 to have 5 to be located in the long hole, 5 to take shape, prevent the experiment result is wasted by 5 paper towel, be located in the barrel 5 is located in the disinfection hole is located 5 at the side 6 at the time.

As one embodiment of the invention, the end of the L-shaped tube 4 far away from the arc groove I3 is provided with the sealing film 14, the diameter of the L-shaped water tube 4 is small, the diameter of the suction tube is small, the liquid level can ensure a certain line shape under the conditions of surface tension, air pressure, liquid dead weight and force generated by external vibration, so that the liquid can keep a certain shape like a solid, just like a plug, and can not flow out under the action of atmospheric pressure, when the sealing film 14 seals one end of the L-shaped tube 4, the detection solution in the L-shaped tube 4 can not flow out from the other end, the sealing film 14 can be made of paper material and can be pricked by the needle 12, one end of the L-shaped tube 4 provided with the sealing film 14 is in threaded connection with the cover 11, the end of the cover 11 far away from the L-shaped tube 4 is provided with the needle 12 for breaking the sealing film 14, the needle 12 is provided with a protective sleeve for preventing bacteria from being stained on the needle 12 and the bacteria from entering the detection solution in the process of pricking the sealing film 14, and causing false detection results.

The bouncing component 6 comprises a first fixing piece 60 and a panel 64; the bottom of the first fixing piece 60 is fixedly connected to the circular groove 8, the top end of the first fixing piece 60 is fixedly connected with a first poking piece 61, a rotating block 62 is arranged between the two first poking pieces 61, and one end of the rotating block 62 is connected with a ratchet wheel 63;

the panel 64 bottom fixedly connected with spliced pole 65, the cover is equipped with mounting three 69 on the spliced pole 65, and the spliced pole 65 bottom is connected with connecting rod 66, and the cover is equipped with mounting four 610 and mounting five 611 on the connecting rod 66, is located between mounting four 610 and the mounting five 611 and is provided with spring 612, and connecting rod 66 bottom fixedly connected with mounting two 67, and mounting two 67 rotates and is connected with plectrum two 68.

The panel 64 is provided with a round hole 613, a movable column 614 is arranged in the round hole 613, a limiting block 615 is arranged at one end of the movable column 614, which is close to the panel 64, and the position of the movable column 614 on the panel 64 is limited, so that the movable column 614 is prevented from falling off through the round hole 613, and the implementation effect of the device is affected.

As one embodiment of the present invention, because the probe is required to be washed between different test solutions, a sterilized paper towel is placed on the panel 64 for washing the probe after it has been extended from the test solution.

Because the sterilized paper towel is placed on the panel 64, when the paper towel is pressed downwards after the surface paper towel is used, the panel 64 drives the compression spring 612 which moves downwards along with the connecting post 65, and simultaneously drives the connecting rod 66 and the second shifting piece 68 to move downwards, and simultaneously drives the ratchet 63 to turn over along with the second shifting piece 68 to move downwards, in the process of turning over the ratchet 63, the protruding position on the ratchet 63 is contacted with the movable post 614, so that the movable post 614 moves upwards and simultaneously drives the sterilized paper towel on the panel 64 to move upwards, and the staff can take the sterilized paper towel conveniently.

As one implementation mode of the invention, the cylinder 2 is provided with the first long groove 9 at the periphery, the first long groove 9 is connected with a transparent plate (not shown in the figure), and the first long groove 9 is internally provided with the names of all detection solutions, thereby being beneficial to the identification of the detection solutions by workers, further being beneficial to the smooth performance of experiments, the upper end surface of the cylinder 1 is provided with the second long groove 10, and the second long groove 10 is internally provided with a sterilized probe.

As one implementation mode of the invention, the number of the arc-shaped grooves I3, the arc-shaped grooves II 7, the L-shaped pipes 4 and the long grooves I9 is the same, the number of the arc-shaped grooves I3 and the like can be set by detecting the number of items, and the positions of the arc-shaped grooves I3, the arc-shaped grooves II 7 and the long grooves I10 are correspondingly on the same straight line, so that identification of detection solutions is facilitated, and identification and acquisition of staff are further facilitated.

As one embodiment of the invention, the cylinder 1 is a round table, that is, the radius of the upper end surface is smaller than that of the lower end surface, the radius of the round groove 8 is consistent with that of the lower end surface of the cylinder 1, that is, the round groove 8 is in sealing connection with the cylinder 1, so that bacteria are prevented from falling into the round groove.

The specific working principle is as follows:

preparation: taking a sample of a patient's gene by blood or saliva in advance, and placing the sample into a sampling tube; and then, different detection solutions and the long groove I9 are inserted with the names of the detection solutions and correspond to the test paper of the solution corresponding to the L-shaped pipe 4, so that the detection solutions can be identified by staff, and the experiment can be further smoothly carried out.

The cover plate I (not shown) on the surface of the arc-shaped groove II 7 and the cover plate II 13 on the rectangular hole 5 are opened, then the cover 11 is taken down, the protective sleeve on the puncture needle 12 is taken down, then the puncture needle 12 is used for puncturing the sealing film 14, the protective sleeve is used for preventing bacteria from being stained on the puncture needle 12, and in the process of puncturing the sealing film 14 by the puncture needle 12, the bacteria enter the detection solution, so that the detection result is wrong.

Because the L-shaped tube 4 has small diameter, the liquid level can ensure a certain line shape under the conditions of surface tension, air pressure, liquid dead weight and force generated by external vibration, so that the liquid can keep a certain shape like a solid, just like a plug, and can not flow out under the action of atmospheric pressure, and when the sealing film 14 is punctured by the puncture needle 12, the solution in the L-shaped tube 4 flows into the arc-shaped groove I3 under the action of gravity.

When the solution in the L-shaped pipe 4 flows into the arc groove I3, the probe is taken out from the long groove II 10, the probe is inserted into the arc groove I3, the lower end of the probe is dipped with the solution, and then test paper in the arc groove II 7 is used for testing.

When the detection is completed once, other solutions need to be detected, the solution on the probe needs to be wiped out by using the sterilized paper towel, and when the paper towel at the uppermost end of the panel 64 is used up, the panel 64 can drive the compression spring 612 which moves downwards through downwards pressing the paper towel, and simultaneously drive the connecting rod 66 and the second shifting piece 68 to move downwards, and simultaneously drive the ratchet 63 to turn over when the second shifting piece 68 moves downwards, in the process of turning over the ratchet 63, the protruding position on the ratchet 63 is contacted with the movable column 614, so that the sterilized paper towel on the panel 64 is driven to move upwards when the movable column 614 moves upwards, and the staff can take the sterilized paper towel conveniently.

The uppermost towel is then thrown into the trash, where a new towel is moved to the uppermost end, which is then used to clean the probe surface solution.

While embodiments of the present invention have been shown and described above for purposes of illustration and description, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives, and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.

Claims (8)

1. A kit for detecting a lung cancer-associated gene mutation, comprising: barrel (1), its characterized in that: the bottom end of the cylinder body (1) is connected with a cylinder body (2), the bottom end of the cylinder body (2) is provided with a circular groove (8), a plurality of arc grooves I (3) are arranged in the cylinder body (2), the bottom end of the cylinder body (1) is provided with an arc groove II (7), be equipped with a plurality of L type pipe (4) in barrel (1), L type pipe (4) one end and arc groove (3) one side intercommunication, be equipped with microscler hole (5) on barrel (1), just be equipped with spring subassembly (6) in being located microscler hole (5) in barrel (1).

2. The kit for detecting a mutation in a gene associated with lung cancer as set forth in claim 1, wherein: the one end that arc wall one (3) was kept away from to L type pipe (4) is provided with sealing film (14), the one end threaded connection that is equipped with sealing film (14) on L type pipe (4) has lid (11), the one end that L type pipe (4) was kept away from to lid (11) is provided with pjncture needle (12), be equipped with the protective sheath on pjncture needle (12).

3. The kit for detecting a mutation in a gene associated with lung cancer as set forth in claim 1, wherein: the bouncing component (6) comprises a first fixing piece (60) and a panel (64); the bottom of the first fixing piece (60) is fixedly connected to the circular groove (8), the top end of the first fixing piece (60) is fixedly connected with a first poking piece (61), a rotating block (62) is arranged between the two first poking pieces (61), and one end of the rotating block (62) is connected with a ratchet wheel (63);

the panel (64) bottom fixedly connected with spliced pole (65), the cover is equipped with mounting three (69) on spliced pole (65), spliced pole (65) bottom is connected with connecting rod (66), the cover is equipped with mounting four (610) and mounting five (611) on connecting rod (66), be located between mounting four (610) and mounting five (611) and be provided with spring (612), connecting rod (66) bottom fixedly connected with mounting two (67), mounting two (67) rotate and are connected with plectrum two (68).

4. A kit for detecting a mutation in a gene associated with lung cancer as claimed in claim 3, wherein: a round hole (613) is formed in the panel (64), a movable column (614) is arranged in the round hole (613), and a limiting block (615) is arranged at one end, close to the panel (64), of the movable column (614).

5. The kit for detecting a mutation in a gene associated with lung cancer as set forth in claim 1, wherein: the cylinder (2) is provided with a first long groove (9) around, and the upper end surface of the cylinder body (1) is provided with a second long groove (10).

6. The kit for detecting a mutation in a gene associated with lung cancer as set forth in claim 1, wherein: the number of the arc-shaped grooves I (3), the arc-shaped grooves II (7), the L-shaped pipes (4) and the long grooves I (9) are the same, and the positions of the arc-shaped grooves I (3), the arc-shaped grooves II (7) and the long grooves I (9) are correspondingly on the same straight line.

7. The kit for detecting a mutation in a gene associated with lung cancer as set forth in claim 1, wherein: the cylinder body (1) is a round table, and the radius of the round groove (8) is consistent with the radius of the lower end surface of the cylinder body (1).

8. Use of a kit according to any one of claims 1-7 for detecting a mutation in a lung cancer gene.